ELECTROANALYSIS
·
2016
·Vol. 28
·期 9
·2044-2049
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IF 2.471
Juanjuan Zuo,Xiaoyu Zhao,Xiaocui Ju,Shue Qiu,Wenshuai Hu,Ting Fan,Juankun Zhang
A novel molecularly imprinted polymer (MIP) sensor for rapid determination of cardiac troponin (cTnI) was established. Since it can bind to the template molecule cTnI specifically, it can be used to detect concentration of cTnI in serum without much sample pretreatment. What's more, the electrochemical signals depend on the concentration of template molecules. The synthetic sensor possesses advantages including simplicity, high specificity, low cost of preparation, good chemical and mechanical properties, sensitive and label-free determination. The synthetic sensor shows good dynamic linearity at concentration range from 0.05 to 5.00 nM. The limit of detection (LOD) was found to be 0.027 nM. The detection time of whole process was within 5 minutes.
研究领域:
生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
INORGANIC CHEMISTRY
·
2013
·
IF 4.593
Yu-Peng Zhou,En-Bao Liu,Jin Wang,Hsiu-Yi Chao
Newly prepared gold(I) acetylide-based luminescent cation sensors 1c and 1d, which bear a novel three–armed flexible conformation, together with control molecule 2c, were synthesized in four steps. 1c and 1d exhibit the π → π* absorption of the acetylide ligands in fluid solutions and produce the 3(ππ*) emission of the acetylide ligands in the solid state and degassed THF solution. The 3(ππ*) emission of 1c in DMSO can be turned on upon addition of Ag+. The binding ratio between 1c and Ag+ as well as the binding constant log K were determined as 1:1 and 4.35 ± 0.12 respectively by UV–vis titration experiments. The formation of [1c·Ag]+ adduct, which leads to the appearance of new up-field peaks, was confirmed by 1H NMR spectroscopic titrations. The control 1H NMR titration experiments for the acetylide-free analogue 1a and single-armed analogue 2c indicate the acetylide groups and tripodal structures are responsible for the binding of Ag+. The control experiment for tripodal gold(I) acetylide analogue 1d suggests the change of PPh3 with P(2-Py)Ph2 induces similar binding affinity toward Ag+ but less selectivity toward Ag+. Free 1c also exhibits the anion binding affinity toward F–, but the Ag+ adduct [1c·Ag]+ shows less affinity toward F–.
研究领域:
分析化学
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材料科学
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配位化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BRITISH JOURNAL OF PHARMACOLOGY
·
2024
·
IF 6.8
Yunfu Shen,You Yang,Zi Wang,Wanjun Lin,Na Feng,Meina Shi,Jiachen Liu,Wenzhe Ma
Background and Purpose Triple-negative breast cancer (TNBC) has a poor prognosis due to limited therapeutic options. Recent studies have shown that TNBC is highly dependent on mitochondrial oxidative phosphorylation. The aim of this study was to investigate the potential of coptisine, a novel compound that inhibits the complex I of the mitochondrial electron transport chain (ETC), as a treatment for TNBC. Experimental Approach In this study, mitochondrial metabolism in TNBC was analysed by bioinformatics. In vitro and in vivo experiments (in mice) were conducted to evaluate the potential of coptisine as an ETC complex I-targeting therapeutic agent and to investigate the molecular mechanisms underlying coptisine-induced mitochondrial dysfunction. The therapeutic effect of coptisine was assessed in TNBC cells and xenograft mouse model. Key Results We demonstrated that mitochondrial ETC I was responsible for this metabolic vulnerability in TNBC. Furthermore, a naturally occurring compound, coptisine, exhibited specific inhibitory activity against this complex I. Treatment with coptisine significantly inhibited mitochondrial functions, reprogrammed cellular metabolism, induced apoptosis and ultimately inhibited the proliferation of TNBC cells. Additionally, coptisine administration induced prominent growth inhibition that was dependent on the presence of a functional complex I in xenograft mouse models. Conclusion and Implications Altogether, these findings suggest the promising potential of coptisine as a potent ETC complex I inhibitor to target the metabolic vulnerability of TNBC. Graphical
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Na Lin,Xin Wang,Hong-Yan Zhang,Kai-Qi Sun,Li Xiao,Xin-Yue Zhang,Cheng-Yang Yue,Li Han,Zhi-Wei Chen,Xiao-Wu Lei
Commercially available rare-earth-doped inorganic oxide materials have been widely applied as X-ray scintillators, but the fragile characteristics, high detection limit, and harsh preparation condition seriously restrict their wide applications. Furthermore, it remains a huge challenge to realize X-ray flexible imaging technology for real-time monitoring of the curving interface of complex devices. To address these issues, we herein report two isostructural cuprous halides of zero-dimensional (0D) [AEPipz]CuX3·X·H2O (AEPipz = N-aminoethylpiperazine, X = Br and I) with controllable size to nanosize crystal as highly efficient scintillators toward flexible X-ray imaging. These cuprous halides exhibit highly efficient cyan photoluminescence and radioluminescence emissions with the highest quantum yield of 92.1% and light yield of 62,400 photons MeV–1, respectively, surpassing most of the commercially available inorganic scintillators. Meanwhile, the ultralow detection limit of 95.7 nGyair s–1 was far below the X-ray dose required for diagnosis (5.5 μGyair s–1). More significantly, the flexible film is facilely assembled with excellent foldability and high crack resistance, which further acts as a scintillation screen achieving a high spatial resolution of 17.4 lp mm–1 in X-ray imaging, demonstrating the potential application in wearable radiation radiography. The combined advantages of high light yield, low detection limit, and excellent flexibility promote these 0D cuprous halides as the most promising X-ray scintillators.
研究领域:
材料科学
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放射学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 9
·3352-3357
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IF 3.269
Wenjuan Guo,Na Sun,Yankai Du,Luyan Wang,Meishan Pei
A poly(glycidyl methacrylate) (PGMA) grafted bentonite (Bent) was prepared by surface-initiated atom transfer radical polymerization (SI-ATRP), followed by modification with tetraethylenepentamine (TEPA) through a ring-opening reaction of the epoxy groups in PGMA to form polyamine grafted bentonite (Bent–PGMA–TEPA). The as-prepared novel material was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and thermal gravimetric analysis (TGA). The ability of Bent–PGMA–TEPA to adsorb Orange I (OI), a negatively charged dye, was tested. The effects of pH, initial concentration, contact time and temperature were investigated. The equilibrium adsorption isotherm and adsorption kinetics fitted well with the Langmuir model and the pseudo-second-order kinetic model respectively.
研究领域:
材料科学
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环境科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiaolei Zhao,Ya Chen,Lu Yu,Zhixiang Xu,Zhonghui Han,Jinxing He
Due to the globalized supply of food and rapid development of the food industry, food contamination has become a leading cause of food safety problems. In this study, a fluorescent sensing system for the fast and sensitive analysis of Sudan I was developed based on hollow molecularly imprinted polymers and a fluorescent encoding technique by using CsPbX 3 perovskite quantum dots as the fluorescent elements. The introduction of hollow structure provided a high loading capacity, faster encoding efficiency, and shorter detection time. After loading Sudan I, the fluorescent intensity was obviously quenched. Under the optimized detection conditions, this analysis method achieved good linearity in the range of 0.1–300 μg L −1 , a limit detection of 0.05 μg L −1 , and spiked recoveries of 94.91 %–105.36 % in food samples. The whole detection process can be completed within 10 min. Importantly, this work also provided a smartphone-assisted intelligent detection platform for the visual and real-time analysis of Sudan I.
研究领域:
食品安全
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Luke Wei,Yuxin Hu,Jiamin Wu,Jing Mao,Tianqin Yin,Jieqiong Qiu
Doxorubicin (DOX), a widely utilized chemotherapeutic antibiotic, increasingly threatens environmental and public health due to its persistence in marine ecosystems, bioaccumulation in organisms, and contamination of agricultural systems. In response to the urgent demand for effective DOX detection, we have developed a label-free fluorescent aptamer biosensor (FAB) that employs SYBR Green I (SG I)-mediated signal amplification. A key innovation in this design is the strategic fragmentation of the 41-mer complementary strand (CP) of the DOX aptamer into three 11-mer fragments. It significantly mitigates nonspecific SG I binding interference caused by the longer ssDNA CP, enhancing the fluorescence signal ratio (F dsDNA /F ssDNA ) from 4.6-fold to 12.6-fold. Upon DOX introduction, competitive binding between DOX, SG I, and DNA further suppresses background noise, yielding an exceptional fluorescence quenching ratio (F 0 /F) of 52.8. The optimized FAB demonstrates ultra-sensitive DOX detection, with a detection limit of 1.41 nM and a linear response between 1.41 nM and 300 nM. The recoveries of DOX spiked into pond water and drinking water samples ranged from 95.1% to 101.8%, with relative standard deviations (RSDs) below 3.46%, demonstrating negligible matrix effects and confirming the FAB method’s robustness for environmental monitoring applications. This strategy synergistically enhances signal transduction efficiency, and strengthens detection reliability, all while maintaining operational simplicity. As a result, this FAB for DOX detection holds transformative potential, poised to revolutionize biosensor technology in the fields of food safety, environmental monitoring, and biosecurity.
研究领域:
生物传感器
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环境科学
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分析化学
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分子生物学
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生物化学
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纳米技术
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公共卫生
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海洋生态学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Guibin Peng,Bixia Lin,Manli Guo,Yujuan Cao,Ying Yu,Yumin Wang
Deoxyribonuclease I (DNase I) is a typical nuclease that plays key roles in many physiological processes and the development of a novel biosensing strategy for DNase I detection is of fundamental significance. In this study, a fluorescence biosensing nanoplatform based on a two-dimensional (2D) titanium carbide (Ti 3 C 2 ) nanosheet for sensitive and specific detection of DNase I was reported. Fluorophore-labeled single-stranded DNA (ssDNA) can be spontaneously and selectively adsorbed on Ti 3 C 2 nanosheet through the hydrogen bond and metal chelate interaction between phosphate groups of ssDNA and titanium of Ti 3 C 2 nanosheet, resulting in effective quenching of the fluorescence emitted by fluorophore. Notably, it was found the enzyme activity of DNase I will be terminated by the Ti 3 C 2 nanosheet. Therefore, the fluorophore-labeled ssDNA was firstly digested by DNase I and the “post-mixing” strategy of Ti 3 C 2 nanosheet was chosen to evaluate the enzyme activity of DNase I, which provided the possibility of improving the accuracy of the biosensing method. Experimental results demonstrated that this method can be utilized for quantitative analysis of DNase I activity and exhibited a low detection limit of 0.16 U/ml. Additionally, the evaluation of DNase I activity in human serum samples and the screening of inhibitors with this developed biosensing strategy were successfully realized, implying that it has high potential as a promising nanoplatform for nuclease analysis in bioanalytical and biomedical fields.
研究领域:
生物化学
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纳米技术
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生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Xiao-Han Pan,Ling-Xiao Fu,Huan Wang,Yi Xue,Jian-Hua Zu
A novel sulfydryl-functionalized adsorbent PP-g-GMA@MEA for recovery of Ag(I) was synthesized by γ-ray induced simultaneous grafting of glycidyl methacrylate (GMA) onto polypropylene (PP) non-woven fabric, followed by ring-opening processes with mercaptoethylamine (MEA). The obtained adsorbents before and after adsorption of Ag(I) were fully characterized by SEM, EDS, FT-IR spectra, XRD and XPS. Batch adsorption experiments were carried out to evaluate adsorption abilities of the adsorbents under high acidity. The adsorption kinetics of Ag(I) were well fitted with pseudo-second-order kinetics model. The maximum adsorption capacity calculated by the Langmuir isotherm model was 262.51 mg/g at 25 °C. EDS, XRD and XPS analyses confirmed the mechanism of adsorption under high acidity was chelating reaction between -SH and Ag(I) and ion exchange adsorption towards [Ag(NO 3 ) 2 ] −1 . Through competitive adsorption tests, the PP-g-GMA@MEA was found remarkably selective towards Ag(I) when it coexisted with Cu(II), Co(II), Ni(II) and Cr(III) in high acidity medium. Therefore, the developed PP-g-GMA@MEA composite would be a promising adsorbent for Ag(I) recovery from industrial effluents.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 36
·36776-36785
·
IF 2.914
Ren Zhichang,Wu Wanlin,Yu Ling,Yu Yang
Thallium as a highly toxic metal element has been listed as one of priority drinking water contaminants. In this study, manganese oxide nanoparticles were synthesized through a simple hydrothermal method and applied for the removal of thallium(I). The adsorbent was composed of numerous needle-like nanorods and had an average volume diameter of 230 nm after heat-drying procedure. The crystal form of adsorbent was determined as α -MnO 2 . The adsorbent exhibited a much faster adsorption rate than most of previously reported adsorbent, achieving over 66.4% of equilibrium adsorption capacity in the first 10 min. The adsorption process was found to be highly affected by solution pH and higher than 100 mg/g of adsorption capacity could be obtained in a wide pH range of 6.0–10.0. The isotherm study indicated that the adsorption of Tl(I) on the adsorbent was favorable and governed by a chemisorption process, with the maximum adsorption capacity of 505.5 mg/g at pH 7.0. The adsorption process was confirmed to be thermodynamically spontaneous and endothermic. The presence of Na + , K + , Mg 2+ , Ca 2+ , and Cu 2+ cations had certain negative effects on the uptake of Tl(I). Based on the batch experiments and XPS analysis, the deprotonated hydroxyl groups that bonded to manganese atoms worked as the binding sites for the effective removal of Tl(I) ions and no redox reaction occurred during the adsorption process.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2023
·
IF 3.6
Haiyun Peng,Yangyi Zhang,Guisheng Deng
Silver(I)-catalyzed tandem reaction of enynones with 4-alkenyl isoxazoles provides access to 2-(furan-2-yl)-1,2-dihydropyridines. No competitive cyclopropanation of alkenes and O–H insertion via (2-furyl)carbene complexes were observed. The cascade reaction proceeds via the formation of (2-furyl)metal carbene intermediate, the N–O bond cleavage of 4-alkenyl isoxazoles/rearrangement, subsequent 6π electrocyclic reaction, and [1,5] H-shift. The successive construction of both 1,2-dihydropyridine skeleton and furan frame has been achieved in the one-pot reaction. A broad range of readily available enynones and 4-alkenyl isoxazoles are suitable to this protocol; however, when R3 is the alkyl group such as n-Bu and Me, a complicated mixture was generated without the desired products. In addition, in the case of R4 = bulky group such as R3′SiOCH2, the reaction gave an in situ oxo-product of (2-furyl)silver carbene. An atom-economic strategy for the synthesis of 2-(furan-2-yl)-1,2-dihydropyridines has been established.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 3
·543
·
IF 3.098
Zhi-Feng Li,Meng-Ying Zhou,Ting Tan,Chen-Cong Zhong,Qi Wang,Shi-Lin Yang,Yu-Lin Feng,Hui Ouyang
Ziyuglycoside I (ZGS1) is a promising drug candidate for the treatment of leucopenia. Currently, information on ZGS1 and its in vivo metabolite ziyuglycoside II (ZGS2) is limited. The objective of this study was to investigate the pharmacokinetics, tissue distribution, and excretion of ziyuglycoside I (ZGS1) and its metabolite ziyuglycoside II (ZGS2) in rats. In our study, a simple and sensitive high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method was established for simultaneous determination of ZGS1 and its metabolite for Sprague-Dawley rat pharmacokinetics studies. The method was validated following internationally-approved guidelines. The results presented in this study indicated that subcutaneous administration of ZGS1 prolonged its extension time and increased the area under the curve (AUC0–t) of ZGS2 during 0 to t minutes. In summary, in this study, the pharmacokinetic characteristics of ZGS1 and its metabolite ZGS2 were defined and its tissue distribution, and excretion in rats were described. Our finding may be beneficial for leucopenia drug that focus on ZGS1.Keywords:ziyuglycoside I (ZGS1);ziyuglycoside II (ZGS2);LC-MS/MS;pharmacokinetics;tissue distribution;excretion
研究领域:
药物代谢
·
药理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）