NATURE BIOTECHNOLOGY
·
2022
·Vol. 40
·期 11
·1601-1609
·
IF 68.164
Chen Ji,Chen Mengyin,Zhu Ting F.
Mirror-image aptamers made from chirally inverted nucleic acids are nuclease-resistant and exceptionally biostable, opening up opportunities for unique applications. However, the directed evolution and selection of mirror-image aptamers directly from large randomized l -DNA libraries has, to our knowledge, not been demonstrated previously. Here, we developed a ‘mirror-image selection’ scheme for the directed evolution and selection of biostable l -DNA aptamers with a mirror-image DNA polymerase. We performed iterative rounds of enrichment and mirror-image polymerase chain reaction (PCR) amplification of l -DNA sequences that bind native human thrombin, in conjunction with denaturing gradient gel electrophoresis (DGGE) to isolate individual aptamers and l -DNA sequencing-by-synthesis to determine their sequences. Based on the selected l -DNA aptamers, we designed biostable thrombin sensors and inhibitors, which remained functional in physiologically relevant nuclease-rich environments, even in the presence of human serum that rapidly degraded d -DNA aptamers. Mirror-image selection of biostable l -DNA aptamers directly from large randomized l -DNA libraries greatly expands the range of biomolecules that can be targeted, broadening their applications as biostable sensors, therapeutics and basic research tools.
研究领域:
分子生物学
·
生物技术
·
纳米技术
·
治疗学
·
传感器
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DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 52
·32786-32794
·
IF 3.108
Jingqian Fan,Yuangang Liu,Shibin Wang,Yulu Liu,Siming Li,Ruimin Long,Ran Zhang,Ranjith Kumar Kankala
Layer-by-Layer (LbL) self-assembly of nanocarriers has garnered the interest of researchers for a wide variety of biomedical applications. In this study, we demonstrated the preparation of poly(lactide-co-glycolide) (PLGA)-(poly-L-ornithine (PLO)/fucoidan)4 core–shell nanoparticles (LbL NPs) by a LbL-based self-assembly process, which possessed a mean size of 170 nm. In LbL NPs, a drug carrying PLGA nano-core is coated with alternating PLO and sulfated polysaccharide fucoidan composite films as a shell on the surface. The anti-tumor drug doxorubicin (DOX) loaded into the PLGA core, resulted in better encapsulation efficiency and its in vitro release from LbL NPs demonstrates that this core–shell strategy takes an advantage of its ability to hold the drug cargo and exhibit controlled release. Further, in vitro cell uptake studies by confocal laser scanning microscopy (CLSM) examination in breast tumor cells (MCF-7 cell line) have confirmed that the nanocarriers are successfully internalized and outlined their presence in the cytoplasm after 4 h of incubation. These intracellularly delivered DOX-loaded LbL NPs exhibited significant anti-tumor activity against breast tumor cells. This innovative chemotherapeutic design taking above advantages of successful internalization along with controlled release property signifies as a promising interventional therapeutic delivery system.
研究领域:
药剂学
·
生物材料
·
肿瘤学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 43
·9031-9040
·
IF 6.331
Jilin Zheng,Peng Zhao,Shiying Zhou,Sha Chen,Yi Liang,Fengchun Tian,Jun Zhou,Danqun Huo,Changjun Hou
Integrating metal–organic frameworks (MOFs) of different components or structures together and exploiting them as electrochemical sensors for electrochemical sensing has aroused great interest. Furthermore, the incorporation of noble metals with MOFs is conducive to the improvement of catalytic performance. In this work, Pd@UiO-66-on-ZIF-L nanomaterials were successfully synthesised onto a self-supported flexible carbon cloth (Pd@UiO-66-on-ZIF-L/CC) through a novel strategy called MOF-on-MOF. Then, Au nanoparticles were electrodeposited onto Pd@UiO-66-on-ZIF-L/CC to obtain Au–Pd@UiO-66-on-ZIF-L/CC, which can serve as an excellent electrocatalyst for the reduction of hydrogen peroxide (H2O2). The obtained flower-like Pd@UiO-66-on-ZIF-L/CC hybrid MOF changes the structure of the monomeric MOF alone and adds more attachment sites. The synergy of the bimetals greatly improved the catalytic performance of the as-developed sensor. Electrochemical experiment results show that the proposed sensor based on Au–Pd@UiO-66-on-ZIF-L/CC has an extended linear range from 1 μM to 19.6 mM with a sensitivity of 390 μA mM−1 cm−2, and a low limit of detection (LOD) of 21.2 nM (S/N = 3). Moreover, it has good anti-interference, reproducibility, repeatability and excellent stability. Furthermore, the real-time in situ detection of H2O2 secreted from human adenocarcinomic alveolar basal epithelial cells (A549 cells) was achieved by culturing cells on Au–Pd@UiO-66-on-ZIF-L/CC, which indicates the potential of the sensor for applications in cancer pathology. Both the synthesis strategy and the sensor design provide new methods and ideas for the production of ultrasensitive H2O2 electrochemical sensors.
研究领域:
材料科学
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电化学
·
生物传感器
·
癌症病理学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2017
·
IF 4.747
Ruichang Gao,Yanmin Wang,Jianlou Mu,Tong Shi,Li Yuan
In this study, the effects of l -histidine (L-His) on the aggregation of bighead carp ( Aristichthys nobilis ) myosin induced by heating in low (0.1) or high (0.5) ionic strength solutions were studied. The solubility of natural myosin was significantly enhanced by L-His both in low and high ionic strength solutions. More small particles are suspended in the heated solution containing L-His, inducing a higher solubility than that solution without L-His. The surface hydrophobicity of myosin in a low ionic strength solution containing L-His is higher than that of myosin without L-His. The results of morphology show that L-His induced myosin to form finer aggregates and a honeycomb-like network in a low/high ionic strength solution during heat treatment. These results demonstrate that L-His might disrupt the electrostatic property of unfolded myosin heat-induced through electrostatic binding with the exposed negatively charged amino acid residues, which might disarrange hydrophobic surfaces, resulting in weakening hydrophobic interactions, which ultimately suppresses the fierce aggregation of carp myosin.
研究领域:
食品科学
·
蛋白质化学
·
水产养殖
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DOI
高影响因子
Water
·
2025
·Vol. 17
·期 22
·3234
·
IF 3
Zeyu Xiong,Peng Jing
Road-marking operations generate alkaline wash water with intense color and soluble cationic additives. A new biomass adsorption material (LML) was developed to address dye pollution in road-marking wash water effectively. Enzymatically hydrolyzed lignin was used as the raw material for the first time. L-lysine was modified to the structure of the lignin benzene ring using a simple one-step synthesis method, which endowed lignin with a large number of active carboxyl and amino functional groups to improve its adsorption capacity. The adsorption performance of LML for methylene blue in water was also investigated. The experimental results show that the LML has a high dye removal rate under alkaline conditions. The fitted adsorption model shows that the saturated adsorption capacity of LML for methylene blue (MB) is 129.4 mg g−1and malachite green (MG) is 244.9 mg g−1, which is in line with the Langmuir isotherm adsorption model. The adsorption process is endothermic, which means that the adsorption capacity increases with increasing temperature. Kinetic studies showed that the adsorption process reached equilibrium within 120 min following a pseudo-second-order kinetic model. The cycle experiment shows that the removal efficiency of the adsorbent for dyes can still reach 90% after five cycles, indicating a good practical application value for the polishing of road-marking wash water.
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产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2024
·
IF 9.3
Lin Fan,Shangshang Sun,Zhidan Zhang,Yanmei Qin,Peter Ruhdal Jensen,Chun You
In conjunction with the pressing global issue of climate change and the associated concern over global warming, increasing interest has emerged in the exploration of carbon dioxide (CO2) as a resource for the generation of a diverse array of products intended to serve societal needs. This study presents the development of an ATP-free and NAD-balanced in vitro synthetic enzymatic biosystem (ivSEB), which comprises only five cascade thermophilic enzymes, designed for the synthesis of L-malate through CO2 fixation powered by the utilization of D-glucose as a substrate. This designed ivSEB yields two moles of L-malate from one mole of D-glucose and two moles of CO2. Through meticulous refinement of reaction conditions and enzyme loading amounts, this ivSEB has demonstrated its capability to produce 6.85 mM of L-malate via CO2 fixation from an initial 5 mM of D-glucose with a molar product yield of 68.5%, and 2.45 mM of L-lactate as a byproduct. In the pursuit of assessing the industrial feasibility of this ivSEB, the study further subjected the system to the utilization of a high concentration (45.70 mM) of D-glucose. Although this endeavor necessitates additional optimization for enhanced efficiency, the present findings herald the emergence of an alternative avenue for the sustainable production of L-malate through CO2 fixation, thus bearing substantial promise for addressing ecological and industrial imperatives.
研究领域:
生物技术
·
环境科学
·
化学工程
·
可持续化学
·
酶学
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hong Wang,Yan Li,Wenli Zhao,Yu Wang,Jinqian Pang,Li Wan,Qipeng Zhang,Wenjuan Liang
Hazardous substances such as acrylamide (AA) are easily produced by the Maillard reaction during coffee roasting. By adding l -cysteine ( l -Cys), the production of AA was inhibited whereas the profiles of the newly formed compounds remain unclear. To elucidate the influence of l -Cys addition on the main characteristic compounds of Yunnan Coffea arabica , investigations using LC-MSⁿ-IT-TOF, FTIR and assays of in vitro antioxidant and xanthine oxidase (XO) inhibitory activities were carried out. The result revealed that the most characteristic compounds represented by the chlorogenic acid analogs (CGAs) were critical to hazards formation during the Maillard reaction. Adding l -Cys before roasting enhanced coffee quality by reducing harmful intermediates such as 5-hydroxymethylfurfural (5-HMF) while increasing bio-potentials. New compound N6 which was formed by the addition of l -Cys and CGAs, together with the flavonoids generated through polymerization and simple phenols derived from thermal cleavage, were mainly contributed to the above potential.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2016
·Vol. 105
·期 6
·1369-1378
·
IF 2.881
Bing Wang,Jun Dong,Lijing Niu,Wenyan Chen,Dongliang Chen,Chengyi Shen,Jiang Zhu,Xiaoming Zhang
Electrospun membranes of poly(p-dioxanone-co- l -phenylalanine) (PDPA) hold potential as an anti-adhesion material. Since adjustable degradation properties are important for anti-adhesion materials, in this study, the in vitro and in vivo degradation processes of PDPA electrospun membranes were investigated in detail. The morphological analysis of these membranes revealed the main degradation conditions of PDPA membranes. The weight remaining and molecular weight variation showed that the overall degradation rate of the membranes could be adjusted by modulating the molecular structure of the PDPAs. Especially, α-chymotrypsin could catalyze the degradation process of PDPAs. Based on these results, the in vitro degradation mechanism was demonstrated, and confirmed by 1 H NMR of the hydrolysis products. Finally, the in vivo degradation and biocompatibility of different PDPAs were investigated. The kinetic study showed that the in vitro and in vivo molecular weight loss of PDPAs have the first-order characteristics. The in vivo degradation rate of the most Phe-containing PDPA-3 is the slowest, and this result relates to the biocompatibilities of PDPAs. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1369–1378, 2017.
研究领域:
生物材料学
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组织工程
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高分子科学
·
生物医学工程
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药物递送
·
再生医学
·
生物降解
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Ying Luo,Jie Xu,Shuo Lv,De-Yang Li,Yuan-Yuan Hu,Xiao-Yang Liu,Fa-Wen Yin,Da-Yong Zhou
Ready-to-eat (RTE) oysters experience rapid and pronounced color deterioration, mainly attributable to lipid oxidation, phenolic oxidation, and Maillard/Maillard-like reactions. RTE oysters were treated with a combination of different color protection agents and packaging strategies to delay browning during storage. The color and associated color-change reactions of RTE oysters were evaluated to identify the optimal color-protection combination and elucidate its regulatory mechanisms. Results indicated that L-cysteine combined with light-proof nitrogen-filled packaging constituted the optimal color-protection strategy. This combination mitigated browning during storage by directly suppressing oxidation and the Maillard reaction. Furthermore, it indirectly inhibited both the Maillard reaction and lipid-mediated Maillard-like reactions by reducing the formation of free radicals and reactive carbonyl compounds. In summary, this study demonstrated that the integration of chemical color-protective agents with cost-effective physical methods effectively delayed the decline in color quality of RTE oysters throughout storage via multiple protective mechanisms.
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2019
·
IF 7.149
Jian Zhou,Zhengshuang Yang,Qing Liu,Yiman Liu,Menglin Liu,Tiecheng Wang,Lingyan Zhu
As emerging alternatives of legacy perfluoroalkyl substances, perfluorophosphinates (PFPiAs) and perfluorophosphonates (PFPAs) are widely applied in industrial and agricultural fields and are supposed to be large partitioned to soil and highly persistent. It is of particular interest to understand their transfer from roots to shoots and transformation in plants, such as wheat. The results of hydroponic experiments indicated that C6/C6 PFPiA, C8/C8 PFPiA, perfluorooctanophosphonic acid (PFOPA), and perfluorohexaphosphonic acid (PFHxPA) were quickly adsorbed on the epidermis of wheat root (Triticum aestivum L.), which was driven by their hydrophobicity. A small fraction of the accumulated PFPiAs and PFPAs in the wheat root was subjected to absorption via an active process dependent on H+-ATPase. PFHxPA, which has the smallest molecular weight and medium hydrophilicity (log Kow < 4), displayed the strongest absorption efficiency via the water and anion channels and had the highest translocation potential from roots to shoots in wheat. C6/C6 and C8/C8 PFPiAs experienced phase I metabolism in wheat, although at a low rate, to form more persistent PFHxPA and PFOPA, respectively, as well as 1H-perfluorohexane (1H-PFHx) and 1H-perfluorooctane (1H-PFO), which were regulated by cytochrome P450 in wheat root. As a result, exposure to PFPiAs in roots ultimately caused the accumulation of more persistent PFPAs in the above-ground parts of plants, raising concerns on their potential risks on human health.
研究领域:
环境科学
·
植物生理学
·
毒理学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Kai Yang,Yueshan Huang,Charles Amanze,Liyi Yao,Richmond Anaman,Bin Huang,Weimin Zeng
The access to the enantiopure noncanonical amino acid l-phosphinothricin (l-PPT) by applying biocatalysts is highly appealing in organic chemistry. In this study, a NADH-dependent glutamate dehydrogenase from Lachnospiraceae bacterium (LbGluDH) was chosen for the asymmetric synthesis of l-PPT. Three flexible loops undergoing big conformational shifts during the catalysis were identified and rationally engineered following the initial mutagenesis. The enzyme’s specific activity toward the key precursor of l-PPT, 2-oxo-4-[(hydroxy) (methyl) phosphinyl] butyric acid (PPO), was improved from negligible to 9 U/mg, and the Km value was reduced to 17 mM. The computational analysis showed that the modified loops broadened the enzyme’s narrow tunnels, allowing the substrate to access the binding pocket and get closer to the crucial residue D165, thereby enhancing the catalytic process. Utilizing the variant as the catalyst, the preparation of l-PPT achieved a 100% conversion rate within 60 min, coupled with a stereoselectivity exceeding 99.9%, demonstrating its practical capacity for industrial application. Similar enhancement in catalytic activity was obtained applying the same strategy to a typical NADH-dependent GluDH from Pyrobaculum islandicum (PisGluDH), indicating the effectiveness of our strategy for the protein engineering of GluDHs targeted to the biosynthesis of unnatural compounds.
研究领域:
生物化学
·
生物技术
·
有机化学
·
酶学
·
蛋白质工程
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DOI
PubMed
高影响因子
International Journal of Pharmaceutics-X
·
2025
·
IF 6.4
Xuekun Wang,Xinyu Zhang,Jin Xu,Shuhai Xu,Keyuan Huang,Qiaoling Ni,Xinyi Shen,Weitao Zhang,Tao Liu,Taimin Dong,Dan Mao,Chunguang Zheng,Wei Yu,Qingcheng Guo,Zhixin Li,Jiawei Cao,Fubo Han,Lusha Ji,Weizhu Qian,Sheng Hou,Jun Li,Ziqiao Sun,Jianxin Dai,Yajun Guo,Dapeng Zhang,Huaizu Guo
The development of high-concentration monoclonal antibody (mAb) formulations for subcutaneous administration is faces critical stability challenges, particularly oxidation and aggregation, which compromise efficacy and safety. While antioxidants are commonly employed, existing studies predominantly focus on low concentrations, leaving the potential of high-concentration antioxidants underexplored. Here, we present the first systematic evaluation of high-concentration L-methionine (L-Met,>20 mM) as a novel antioxidant to address these limitations. Through accelerated stability testing coupled with multi-dimensional analytical techniques, we demonstrated that L-Met at concentrations exceeding 20 mM surpasses conventional antioxidants in mitigating oxidation and aggregation. Synergy with 200 mM trehalose further enhanced stability by reducing oxidative degradation and inhibiting protein aggregation. Comprehensive biophysical analyses confirmed no adverse effects, with some aspects showing improved outcomes in structural integrity, colloidal stability, and thermal behavior. The optimized formulation (25 mM L-Met +200 mM trehalose) also exhibited robust protection against light-induced degradation and broad applicability across therapeutic antibodies. This work pioneers a high-concentration antioxidant strategy, addressing a critical gap in mAb formulation science and offering a translatable solution for stabilizing next-generation high-concentration biologics.
研究领域:
药学科学
·
生物化学
·
生物物理学
·
制剂开发
·
抗氧化治疗
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）