CYTOTECHNOLOGY
·
2021
·Vol. 73
·期 3
·483-496
·
IF 2.058
Lu Hengxiao,Wang Hao,Sun Peidao,Wang Jiang,Li Shuhai,Xu Tongzhen
We investigated the role of miR-522-3p in thymoma-associated myasthenia gravis (TAMG), and the mechanism of action in T cells. The miR-522-3p expression in normal serum, non-thymoma MG patient serum and TAMG patient serum and tissues was detected by quantitative real-time PCR (qRT-PCR), respectively. We assessed miR-522-3p expression in Jurkat cells and human CD4 + T cells after activation by anti-CD3 and anti-CD28 using qRT-PCR. The viability, proliferation, cycle distribution and the levels of CD25, CD69, interleukin-2 (IL-2) and IL-10 in transfected Jurkat cells were detected by Cell counting kit-8, 5-ethynyl-2′-deoxyuridine (EdU), flow cytometry, qRT-PCR, respectively. Targeting relationships of miR-522-3p and SLC31A1 were predicted and validated by bioinformatics analysis and dual-luciferase reporter. The viability, proliferation, cycle distribution and the levels of SLC31A1, CD25, CD69, IL-2 and IL-10 in transfected Jurkat cells were detected by above methods and western blot. The miR-522-3p expression was declined in TAMG and activated T cells. MiR-522-3p inhibitor promoted cell viability, EdU positive cells, cycle progression, and the level of CD25, CD69, IL-2 and IL-10 in Jurkat cells, while the effect of miR-522-3p mimic was the opposite. SLC31A1 was targeted by miR-522-3p, and miR-522-3p inhibited SLC31A1 expression. Overexpressed SLC31A1 reversed the inhibitory effects of miR-522-3p mimic on cell viability, EdU positive cell, cycle progression, and the levels of IL-2 and IL-10 in transfected Jurkat cells. MiR-522-3p expression was down-regulated in TAMG, and miR-522-3p inhibited proliferation and activation by regulating SLC31A1 expression in T cells.
研究领域:
分子生物学
·
免疫学
·
医学遗传学
✓ 已验证
DOI
PubMed
高影响因子
Cell Reports
·
2026
·Vol. 45
·期 2
·116933
·
IF 6.9
Chunyu Feng,Su-Ran Li,Xiaoshuai Xu,Zihan Dang,Zihan Jiang,Erhui Jiang,Zhengjun Shang
Summary The role of neurotransmitters in suppressing anti-tumor immunity has garnered increasing attention. While glutamate has been extensively studied in neurological diseases, its potential role in regulating anti-tumor immunity, particularly in the context of neoadjuvant immunochemotherapy, remains underexplored. In this study, we find that glutamate levels are elevated in the plasma of head and neck squamous cell carcinoma (HNSCC) patients. The METTL3/m6A/CD98 axis in cancer-associated fibroblasts (CAFs) is a key driver of glutamate secretion. Glutamate induces CD8 + T cell exhaustion through SLC1A3 and impairs the formation of immune memory in secondary lymphoid structures. Additionally, glutamate promotes ferroptosis resistance in HNSCC. Notably, glutamate depletion enhances the efficacy of neoadjuvant immunochemotherapy. Our findings provide insights into how the METTL3/m6A/CD98 axis-mediated regulation of glutamate efflux may sensitize HNSCC to neoadjuvant immunochemotherapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemical Engineering and Processing-Process Intensification
·
2022
·
IF 4.264
Kai Zhu,Wenjie Ye,Meng Hu,Yiping Huang,Jingjing Huang,Hanfei Liu,Hao Zhu,Zhenhao Wei,Feng Zhou
4-nitroaniline has been widely used in the production of antioxidants, dyes and pharmaceuticals. A continuous-flow strategy was exploited for the efficient synthesis of 4-nitroaniline through the noncatalytic amination of 4-chloronitrobenzene with aqueous ammonia in a highly controlled and safe manner, in which N-methyl-2-pyrrolidone was selected as the appropriate solvent. Various conditions (reaction temperature, residence time, 4-chloronitrobenzene concentration, molar ratio of ammonia to 4-chloronitrobenzene, presence of base catalyst) were investigated to improve the synthesis of 4-nitroaniline. The Box-Behnken design in response surface methodology was employed for the optimization of reaction conditions, and over 91% yield of 4-nitroaniline could be achieved. Furthermore, a kinetic model was proposed to guide the design and optimization of the synthesis of 4-nitroaniline.
研究领域:
有机合成
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2015
·
IF 2.679
Yujuan Chen,Kelei Zhuo,Jing Chen,Guangyue Bai
Apparent molar volumes V Φ ,DIL and viscosity B -coefficients of the dicationic ionic liquids (DILs) in {glucose (Glc) + water} solutions were determined from density and viscosity measurements at T = 298.15 K. Limiting apparent molar volumes V Φ ,DIL 0 of the DILs, volumetric interaction parameters, and activation energies Δ μ DIL 0 ≠ were also obtained, together with the contributions of the solvent property B 1 and the dicationic ionic liquid – solvent interaction B 2 to the B -coefficient. A linear relation was observed between V Φ ,DIL 0 and the number of methylene groups n (CH 2 ) in the alkyl linkage chain of DILs. Volumetric interaction parameters and B 2 values are positive and increase with increasing the length of alkyl linkage chain of the dication. The DIL – glucose interactions were discussed in terms of the size and solvation of the DILs and glucose.
✓ 已验证
DOI
高影响因子
Nano Select
·
2021
·Vol. 2
·期 11
·2135-2147
·
IF 0
Puhong Wen,Xiaowen Jiang,Qian He,Dengwei Hu,Lijun Ren,Weixing Zhao
The tetragonal phase Nb 2 O 5 (T-Nb 2 O 5 ) nanobelts with preferentially exposed the (010) facet have been successfully prepared for the first time by insitu topological reaction using the KNb 3 O 8 nanobelts as the precursor. Meanwhile, the transformation mechanism of the crystal structure and morphology from the layered KNb 3 O 8 nanobelt to the T-Nb 2 O 5 nanobelt was revealed in detail by using XRD, FE-SEM, TEM, HRTEM, SAED, UV-vis DRS, EDX, XPS, and Raman spectra. In addition, Ag deposited T-Nb 2 O 5 (Ag/T-Nb 2 O 5 ) nanobelts were prepared by photo-reduction reaction. The photoelectrochemical properties of the T-Nb 2 O 5 nanobelts and Ag/T-Nb 2 O 5 nanobelts as a photocatalyst were evaluated by splitting water under simulated sunlight. The results show that the photocurrent density generated by the prepared Ag/T-Nb 2 O 5 sample is 48.1 µA∙cm −2 , which is 2.3 times that of the T-Nb 2 O 5 sample, and the prepared tetragonal phase T-Nb 2 O 5 nanobelts produce a photocurrent value 1.5 times that of the contrast orthogonal phase O-Nb 2 O 5 nanosheets. It indicates that the (010) facet of the tetragonal phase Nb 2 O 5 is one of the highest photocatalytic active surfaces, and the visible light utilization efficiency of the T-Nb 2 O 5 nanobelt with Ag deposited on the surface has been significantly improved, which implies that it has potential applications in the field of photocatalysis.
研究领域:
材料科学
·
纳米科技
·
光催化
·
能源化学
·
半导体材料
·
表面科学
✓ 已验证
DOI
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Feifan Cheng,Shihan Huang,Chunlian Zhai,Qian Li,Wei LV,Xueqin Zhao,Biao Huang
Background Time-resolved fluorescence immunoassays (TRFIA) have been extensively applied across various fields owing to their low background noise characteristics, particularly in disease diagnosis and prevention. Survival and prognosis of patients with acute myocardial infarction (AMI) are highly dependent on the rapid and accurate detection of myocardial damage. Troponin T (cTnT) is a primary diagnostic biomarker of AMI and is widely used in clinical practice. However, the current bioassays for cTnT detection are complex and time-consuming. Therefore, there is an urgent need for a sensitivity, and rapid diagnostic approach for acute myocardial infarction. Results Herein, we report a novel, sensitive, and specific cTnT detection approach using a unique nanoprobe dissolution-enhanced fluorescence amplification strategy. Eu2O3 nanocrystals, easily dissolvable in an acidic enhancer solution and transformed into many highly luminescent Eu3+ micelles, were utilized as nanoprobes. The released Eu3+ further activates the time-resolved fluorescence system, which prevents autofluorescence interference and blood scattering, allowing for the accurate determination of cTnT levels in clinical serum samples. The demonstrated sensitivity of serum cTnT was high with a sensitivity of 3.86 ng·L−1. In addition, we developed a Eu2O3-based lateral flow immunochromatographic test strip for facile cTnT detection, with the sensitivity of 14.14 ng·L−1 and 6.31 ng·L−1 using smartphone-integrated app and commercial analyzer respectively. Significance This Eu 2 O 3 -nanocrystal-based dissolution-enhanced luminescent bioassay has tremendous potential for ultrasensitive biomarker detection, enabling ultra-trace detection through lanthanide-specific dissolution. Moreover, the surface-functionalized nanocrystals could selectively recognize a variety of analytes, and have excellent photostability with minimal matrix interference, expanding the direction of lanthanide nanoprobes in multifunctional diagnostic and therapeutic areas.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Reports Medicine
·
2025
·
IF 10.6
Feixiang Wang,Rujuan Bao,Shuiyu Xu,Wenyan Li,Haiyan Huang,Runchang Li,Xinyu Ding,Yuerong Zhang,Xiaoyan Yu,Qiaoqiao Han,Xian Du,Jie Wan,Song Li,Yichuan Xiao,Ren Zhao,Xingang Cui,Youqiong Ye,Jiayuan Sun,Junke Zheng,Guo-Qiang Chen,Qiang Zou
T cell dysfunction with age underlies an increased incidence of cancer in elderly individuals; however, how T cell aging is triggered in the tumor microenvironment is unclear. Here, we show that an age-associated reduction in adipocyte-derived leptin contributes to the accumulation of tumor-infiltrating senescent CD8 + T cells. Single-cell profiling of human and mouse cancer tissues reveals that the frequency of intratumoral senescent CD8 + T cells increases with age, leading to a weak antitumor effect. Moreover, decreased levels of adipocyte-derived leptin are an indispensable factor for CD8 + T cell aging. Leptin signaling prevents p38-dependent CD8 + T cell senescence. Furthermore, plasma leptin levels are negatively related to intratumoral CD8 + T cell senescence in cancer patients. Our findings identify an unappreciated interplay between metabolic perturbation and T cell aging and suggest that modulating adipocyte-derived leptin levels may be a promising therapeutic strategy for older cancer patients.
研究领域:
免疫学
·
癌症生物学
·
细胞生物学
·
老年学
·
代谢研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Xiaolong Yi,Yaning Tang,Yanlin Huang,Xin Wang,Jin Jiang,Weixin Deng,Kuo Jiang
Nano-nano t'-YSZ-Al 2 O 3 composites were synthesized in situ using sol-spray pyrolysis process. The composite powders were sintered into dense ceramics by spark plasma sintering at 1300 °C. The toughness of the composite ceramics was studied, and the microstructure was characterized in detail using X-ray diffraction spectroscope (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM). Microstructural analysis revealed that α-Al 2 O 3 , with a grain size of approximately 100 nm, was distributed along the grain boundaries of YSZ. Notably, the toughness of the YSZ-Al 2 O 3 composite ceramics with 10 wt% Al 2 O 3 addition (85±5 J/m 2 ) was nearly twice that of single-phase YSZ (43±3 J/m 2 ). It is suggested that the nano-toughening can be comparable to ferroelastic toughening. Materials with enhanced toughness can significantly improve the thermal cycling life of coatings, indicating that nano/nano YSZ-Al 2 O 3 composites hold considerable potential for practical applications.
✓ 已验证
产品关联
DOI
高影响因子
Translational Cancer Research
·
2025
·Vol. 14
·期 10
·6454
·
IF 1.7
Jiaxuan Zhao,Han Wu,Zhe Sun,Yeting Cui,Nan Liu,Yixuan Wang,Youyong Wang,Jiangzhou Shi,Tongcun Zhang
Background Chimeric antigen receptor T cell (CAR-T) therapy targeting cluster of differentiation 19 (CD19) has shown promise in treating B-cell acute lymphoblastic leukemia (B-ALL), but the long-term efficacy of CD19-targeted CAR-T therapy remains limited. Programmed cell death protein 1 (PD-1), an inhibitory receptor on activated T cells, engages programmed death ligand 1 (PD-L1) in the tumor microenvironment, suppressing CAR-T cell function by impairing key signaling pathways. To address this limitation, we investigated the effect of short hairpin RNA (shRNA)-mediated PD-1 knockdown on alleviating CAR-T cell exhaustion, preserving memory phenotype, and enhancing the long-term efficacy of CD19-targeted CAR-T cells. Methods To overcome this limitation, we employed shRNA to achieve PD-1 knockdown in CD19-targeting CAR-T cells. Following screening multiple shRNA candidates, we assessed the cytotoxicity, cytokine secretion, and proliferation of PD-1 knockdown CAR-T cells. Their memory phenotype, exhaustion marker, and cytolytic function were further evaluated after repeated antigen stimulation. Therapeutic efficacy and safety were validated in NOD.Cg-PrkdC scid Il2rg tm1 /Vst (NPG) mice. Results PD-1 knockdown significantly enhanced CAR-T cell cytotoxicity and persistence against PD-L1+ RAJI (human Burkitt’s lymphoma cell line) cells. After three rounds of antigen stimulation, PD-1 knockdown CAR-T cells sustained cytotoxic activity, retained a memory-like phenotype, and displayed attenuated exhaustion markers. In vivo , they prolonged survival without inducing cytokine release syndrome (CRS), indicating improved efficacy and safety. Conclusions PD-1 knockdown mitigates tumor-induced T-cell exhaustion and represents a promising strategy to enhance the durability and potency of CAR-T cell therapy in cancer treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2018
·Vol. 6
·期 3
·633-641
·
IF 5.831
Pingzhang Chen,Yunhong Liu,Jing Zhao,Xiaojuan Pang,Peifa Zhang,Xiaohu Hou,Ping Chen,Cheng-yi He,Zhiyong Wang,Zhi-ying Chen
Bispecific T-cell engagers (BiTEs) are single chain variable fragments with specific structures, which could connect the surface antigen on cancer cells and CD3 ligands on T cells, and then engage the T cells for cancer immunotherapy. In this report, a novel organic–inorganic hybrid gene delivery system composed of stearic acid modified polyethyleneimine (stPEI) and calcium phosphate (CaP) was used to deliver MC.DNA into cells to express BiTE antibodies. This gene delivery system exhibits high transfection efficiency, long-term effects and low cytotoxicity in vitro. Furthermore, the gene production, anti-IGF1R/CD3 bispecific T-cell engager, exhibited a rapid redirection activity in T cells to induce cancer cell apoptosis. In summary, the results confirmed that stPEI-CaP could be an efficient gene delivery system for BiTE encoding MC.DNA based gene immunotherapy.
研究领域:
药学
·
生物技术
·
癌症免疫疗法
·
基因递送系统
✓ 已验证
DOI
PubMed
高影响因子
MOLECULAR AND CELLULAR BIOCHEMISTRY
·
2026
·
IF 4.7
Yang Di,Liu Jia,Gao Yan,Guan Xin,Tong Rui
Cervical cancer (CC) is a prevalent malignancy in women; however, the efficacy of immunotherapy in this disease is suboptimal, highlighting the need for novel therapeutic targets. We found that low RNF125 expression correlated with poor prognosis in CC patients. In vitro and in vivo, RNF125 inhibited proliferation and induced apoptosis of CC cells. In immunocompetent mice, RNF125 suppressed tumor growth, increased CD8⁺ T cell infiltration, elevated IFN-γ secretion, and reduced PD-1 expression on CD8⁺ T cells. Co-culture experiments confirmed that tumor cells overexpressing RNF125 enhanced CD8⁺ T cell proliferation and function. Further mechanistic investigation revealed that RNF125 promoted CD8⁺ T cell activity by ubiquitinating and degrading PD-L1. Additionally, RNF125 was identified as a direct target of miR-574-5p. This study demonstrates that RNF125 exhibits tumor-suppressive functions in CC by enhancing anti-tumor immunity, highlighting its therapeutic potential.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2026
·
IF 6.8
Min Cai,Congyu Wu,Muqing Wang,Qian Chen,Yilin Guo,Yu Liu,Xin Huang,Yuan Gao,Yun Qi
Ethnopharmacological relevance Elephantopus scaber L. ( E. scaber ) is a heat-clearing and detoxifying traditional Chinese medicine. Clinically, it is used for treating some immuno-inflammatory diseases associated with excessive T cell activation. However, its action and mechanism on T cells are still ambiguous. Aim of the study This study aims to investigate the molecular mechanism of the ethanol extract of E.scaber (ESE) in ConA-induced T cell activation models. Materials and methods IL-2 transcription and translation levels were determined by RT- q PCR and ELISA. CD4 + naïve T cells were sorted using the flow cytometer. Western blot assay was used for determining intracellular protein levels. Cytoplasmic and mitochondrial Ca 2+ concentrations were measured by fluorometric assays. Results ESE not only dampened the proliferation of splenic lymphocytes, but also decreased IL-2 production in ConA-activated T cells without affecting its mRNA stability. It could inhibit the activation of NFAT and NF-κB pathways, showed as impeding the nuclear translocation of NFAT and p65. Regarding the upstream signaling, ESE significantly decreased cytoplasmic Ca 2+ (Ca 2+ [c] ) level in a model-independent manner. Although it failed to affect plasma membrane Ca 2+ -ATPase, Na + /Ca 2+ exchanger, and mitochondrial Ca 2+ uptake, it affected sarco/endoplasmic Ca 2+ -ATPase (SERCA) pathway. It also hindered IκBα phosphorylation and subsequent degradation. In vivo , ESE reduced IL-2 levels in local and systemic T cell activation models. Conclusion Through inhibiting the activation of SERCA/Ca 2+ [c] /NFAT and NF-κB pathways, ESE markedly decreased ConA-induced IL-2 production in vitro and in vivo . This study provides not only a modern explanation for the traditional uses of E. scaber , but also a scientific guidance for clinical practice.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）