Science of Advanced Materials
·
2018
·Vol. 10
·期 12
·1729-1736
·
IF 1.318
Fan Youhua,Wu Zhenming,Sun Tiantian,Zhan Haijuan,Liu Wanyi,Bi Shuxian
Horseradish peroxidase (HRP) was successfully immobilized on a novel enzyme carrier, hydride composite of graphene oxide grafted sodium styrene sulfonate (GO-g-PSStNa), and used for removal of phenol from water. Hydride composite (GO-g-PSStNa) was synthesized using graphene
oxide (GO) and sodium styrene sulfonate (SStNa) by atom transfer radical polymerization (ATRP). The morphologies and chemical composition of the resulted samples were investigated by SEM, FTIR and XPS. The results showed that the hydrophilic monomer SStNa was grafted onto the surface of the
GO. HRP was immobilized on the surface of hydride material and its loading reached 8.0 mg/g under the immobilization conditions of pH 7 at 0 °C. The removal efficiency of phenol for immobilized HRP had an optimum pH 6, which was shifted to 8 for free enzyme. Both the free and immobilized
enzyme shared the same optimal temperature at 40 °C. Immobilized HRP exhibited higher removal efficiency for phenol compared with free HRP. The best immobilization effect could be reused at least eight times. The high phenol removal can be resulted by improvement of catalytic activity
of immobilized HPR with adsorption on GO-g-PSStNa support.
研究领域:
生物化学
·
环境科学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Hongbo Suo,Lili Xu,Yu Xue,Xiang Qiu,He Huang,Yi Hu
In this work, ionic liquids-modified magnetic carboxymethyl cellulose nanoparticles (IL-MCMC) were prepared and used as supports for enzyme immobilization. The specific activity of immobilized lipase PPL-IL-MCMC was 1.43 and 2.81 folds higher than that of free PPL and PPL-MCMC, respectively. Water contact angle analysis indicated that the introduction of ionic liquids increased the hydrophobicity of supports, which in tune induced the lid-opening of lipase, allowing its active sites to become more accessible. In addition, the affinity between lipase and substrate immobilized on the prepared supports was enhanced. The same method was also applied to analyze immobilize penicillin G acylase (PGA) to further investigate the general applicability of the method. The results showed that the immobilized PGA exhibited higher stability than many other reported PGAs. The developed composites may be utilized as excellent supports for enzyme immobilization in industrial application.
研究领域:
生物化学
·
材料科学
·
酶工程
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 11
·8391-8399
·
IF 3.201
Yajie Wang,Qiang Wang,Xiaoping Song,Jingjing Cai
Dextranase is a unique biocatalyst for the enzymatic hydrolysis of dextran in carbohydrate chemistry, but the instability and poor recycling ability of dextranase significantly limit its long-term applications. As an alternative method, stabilization of dextranase on a solid carrier is a promising strategy. In this study, a kind of polyethylenimine (PEI) polymer modified magnetic particle was fabricated and employed as the substrate for covalent immobilization of dextranase (Fe3O4@SiO2-PEI-dextranase) to improve the stability and reusability performance for the first time. The grafted PEI polymer on the surface enhanced the amount of primary amine groups, and improved the loading capacity of dextranase on Fe3O4@SiO2-PEI-dextranase to 217.2 mg g−1, which was 3.84 times that measured on Fe3O4@SiO2-NH2-dextranase particles. Under the optimized conditions, high immobilization efficiency (72.38%) and enzymatic activity (82.84%) of Fe3O4@SiO2-PEI-dextranase were achieved. Thermal and pH tolerance, and long-term storage tests revealed that the Fe3O4@SiO2-PEI-dextranase exhibited much better stabilities. More importantly, Fe3O4@SiO2-PEI-dextranase retained 83.26% of its initial activity after reuse 15 times, suggesting its excellent reusability. It is believed that the immobilized dextranase could have potential applications in biological and biocatalytic fields.
研究领域:
生物化学
·
生物技术
·
酶学
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DOI
高影响因子
RADIOCHIMICA ACTA
·
2022
·
IF 2.083
Lei Li,Ran Ma,Xuewei Liu,Tao Wen,Bo Wu,Mingtai Sun,Zheng Jiang,Suhua Wang,Xiangke Wang
Inorganic ion-exchange materials show potential application for toxic radioactive ions due to their remarkable high efficiency and selectivity features. Here, two type of carbon-supported titanate (C@TNFs and C@TNFs(H)) nanofibers have been synthesized by a cost-effective in suit growth method. The resulting C@TNFs and C@TNFs(H) microspheres present uniform flower-like morphology and large surface area. The interlayer Na + in the titanate shell provides docking sites for ion-exchange of radioactive ions (U(VI), Ba(II), and Sr(II)). Interestingly, the exceeding theoretical cation-exchange capacities (CECs) are achieved on C@TNFs for U(VI) ∼4.76 meq g −1 and Ba(II) ∼2.65 meq g −1 and C@TNFs(H) for Ba(II) ∼2.53 meq g −1 and Sr(II) ∼2.24 meq g −1 , respectively. The impressive adsorption performance is mainly attributed to the synergistic effects of ion-exchange and surface complexation. More significantly, C@TNFs and C@TNFs(H) maintain high distribution coefficients ( K
d
U ) of >104 mL g −1 over a wider pH range (pH = 3.5–9.0) and high adsorption rate with short equilibrium time within 50 min. Competitive ion-exchange investigation shows a selectivity order of U(VI) > Ba(II) > Sr(II) at individual 10 ppm concentration, pH = 6.0 and T = 298 K. The related spectroscopic studies reveal the intercalative mechanism of radionuclides in the deformed titanate structure, as a result of target ions firmly trapped in the interlayer of C@TNFs and C@TNFs(H). These advantageous features allow the C@TNFs and C@TNFs(H) to be promising candidates for the remediation of toxic radioactive ions polluted water.
研究领域:
材料科学
·
环境科学
·
放射化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Peng Liao,Binrui Li,Lin Xie,Xiaoao Bai,Han Qiao,Qianqian Li,Biwei Yang,Chongxuan Liu
Engineered nanoparticles-mediated contaminant transport has been recognized as a significant process governing the mobility of metals and radionuclides in groundwater. Engineered silicate nanoparticles (ESNPs) are attractive materials for the sequestration or extraction of Cr(VI) and other metals and radionuclides from groundwater. While great efforts have been devoted toward the application of these materials for Cr(VI) sequestration, the underlying interface adsorption mechanism is not thoroughly elucidated. This study investigates the immobilization mechanisms of Cr(VI) on a representative ESNPs, NH 2 -MCM-41, over a range of water chemistry conditions. By combining batch adsorption experiments with an array of complementary characterizations, we provided spectroscopic and microscopic evidence that the electrostatic interactions between the positively charged NH 2 -MCM-41 surface derived from amino functionality and the negatively charged Cr(VI) species was the dominant mechanism responsible for Cr(VI) immobilization. In addition, the weak hydrogen bonding interactions may also contribute to adsorption to a degree. Furthermore, thermodynamic studies suggested a favorable, spontaneous, and exothermic adsorption process. Site energy analysis illustrated that the distribution of energy binding sites on NH 2 -MCM-41 is Cr(VI) loading dependent. The new insights provided here can advance understanding of the transport of Cr(VI) associated NH 2 -MCM-41 that benefits the application of ESNPs-based technologies for metals immobilization in groundwater.
研究领域:
环境科学
·
纳米技术
·
污染治理
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DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 12
·期 4
·2507-2514
·
IF 6.97
Yipeng Bao,Jin Wang,Qi Wang,Xiaofeng Cui,Ran Long,Zhengquan Li
Harvesting solar energy to convert carbon dioxide (CO2) into fossil fuels shows great promise to solve the current global problems of energy crisis and climate change. To achieve this goal, it is desirable to develop efficient catalysts with visible light response to cater for the solar spectrum. CdTe QDs are ideal candidates for absorbing visible light, but it is difficult to directly perform CO2 reduction due to the lack of effective catalytic sites. Herein, we report a strategy for the activation of mercaptopropionic acid (MPA)-capped CdTe QDs for visible-light-driven CO2 reduction, in which iron ions (Fe2+) are immobilized onto CdTe QDs using L-cysteine as a bridging ligand (CdTe-b-Fe). This ligand bridging strategy can immobilize Fe2+ ions on the surface of CdTe QDs as catalytic sites, and these catalytic sites can be conveniently adjusted by directly adding different types or numbers of metal ions. In addition to effectively immobilizing catalytic sites, the bridging ligands can also provide a pathway for electron transport between CdTe QDs and the catalytic sites. The CdTe-b-Fe QD system based on the ligand bridging strategy exhibits excellent catalytic properties: the yield of CH4/CO (two products together) is 126 μmol g−1 h−1, and the selectivity for carbon-based products approaches 98%. This work presents a facile strategy for immobilizing catalytic sites on QDs and provides a platform for designing efficient visible-light driven catalysts for CO2 reduction.
研究领域:
化学
·
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2026
·
IF 3.7
Zhe Wang,Ziai Xing,Xiaomei Ma,Yiyuan Fu,Yucheng Jiang
In this work, hierarchical covalent organic frameworks (H-COF (PS)) were prepared via a hard template strategy using polystyrene spheres (PS) as the template. The H-COF (PS) exhibits high stability in aqueous solution without skeleton collapse. When used as a carrier for enzyme immobilization, the H-COF (PS) shows several outstanding advantages. The well-defined macropores and micropores are distributed throughout the COF. The created macrospores provide sufficient space for distribution of enzyme while the intrinsic micropores can concentrate substrates. The macropores and micropores in H-COF (PS) are adjacent, so the substrate can access to horseradish peroxidase (HRP) with lower diffusion resistance than that in solution, resulting in enhanced enzymatic catalytic activity. The porous skeleton in H-COF (PS) can protect the embedded enzyme from inactivation caused by high temperature and organic solvents, ensuring HRP stability under strict reaction conditions. The prepared HRP immobilized H-COF (PS) (HRP@H-COF(PS)) demonstrates high efficiency in dye decolorization applications. This work provides a new platform to design enzyme-nanomaterials composites with both high activity and stability. Graphical Hierarchical Covalent Organic Frameworks (H-COF (PS)) are prepared using Polystyrene Spheres (PS) template. Created macrospores provide space for enzyme while the micropores concentrate substrates. The porous skeleton protects the loaded enzyme from inactivation at high temperature and in organic solvents. The horseradish peroxidase loaded is efficient in dye decolorization and 2,4-dichlorophenol degradation.
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2026
·
IF 6.1
Tai Ye,Shuying Yue,Long Bai,Haohao Chen,Chenyang Li,Rehemutula Wubuli,Kehua Dang,Min Yuan,Hui Cao,Ziqing Ye,Fei Xu
Co-immobilization of horseradish peroxidase (HRP) with G-quadruplex/hemin complex often suffer from low efficiency, intricate procedures, and reduced catalytic performance. To address these challenges, we developed a biomineralization strategy that integrates HRP and G-quadruplex/hemin into the single nanosheet structure. By engineering a split aptamer sequence into a G-rich domain, the nanosheets synergistically combine enhanced catalytic activity with better target-specific molecular recognition. Compared with either free HRP or it biomineralized counterpart alone, the prepared nanosheets exhibited markedly improved peroxidase-like activity. In the presence of enrofloxacin, specific aptamer-target binding facilitated the formation of a ternary complex on the electrode surface, enabling the catalysis of H 2 O 2 -mediated oxidation of TMB. Under the optimized conditions, the electrochemical aptasensor displayed a wide linear detection range of 1 nM–10 μM with a low detection limit of 0.53 nM. Beyond high selectivity, the sensor demonstrated excellent practical applicability in spiked milk and egg white samples. The generality of the developed platform was further confirmed by its successful extension to other antibiotics detection through replacing the split-aptamer pairs.
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产品关联
DOI
PubMed
高影响因子
FIBERS AND POLYMERS
·
2021
·Vol. 22
·期 9
·2440-2446
·
IF 2.153
Ju Lin-Xin,Li Fan,Ning Xin,He Hong-Wei,Zhou Rong
As a natural cellulose fiber, kapok fiber is much easier to degrade due to its thin wall structure of fiber in the process of storage and use. In this work, a finishing modification was designed and developed to prevent kapok fiber from degradation. Dopamine was polymerized in situ and deposited on the surface of kapok fiber, which was operated in the mixed solvents of ethanol:water (1:1 in volume) to overcome the strong buoyancy. Polyethyleneimine (PEI) was introduced to assist dopamine polymerization and improve the load rate. An optimum dosage, 1.0 g/ l of PEI could afford the highest loading rate of 32.1% of polydopamine (PDA), and remarkably improve loading fastness. As a multi-amino polymer, PEI could reduce Ag + and chelate with metal silver (Ag). The PDA/Ag covered kapok fiber and nonwovens showed good antibacterial activity and better mechanic strength, which would be more suitable for durable oil absorption and sound absorption materials than pure kapok fiber.
研究领域:
材料科学
·
纺织工程
·
生物学
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DOI
高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Ruobing Zhang,Yimin Zhang,Tao Liu,Qian Wan,Dalong Zheng
In recent years, the use of spent fluid catalytic cracking (SFCC) catalysts to prepare geopolymers has received attention from academic and industrial circles. However, SFCC catalysts have been classified as hazardous waste in China due to their vanadium and nickel content. In this paper, the immobilization rates of vanadium and nickel in geopolymers prepared with SFCC catalysts were studied through a toxicity characteristic leaching procedure (TCLP). The immobilization mechanism of nickel and vanadium during geopolymerization was investigated by XPS, SEM, XRD , and compressive strength measurements. The nickel and vanadium TCLP concentrations of the SFCC catalyst-based geopolymer are 0.032 mg/L and 10.63 mg/L, respectively. Nickel in the SFCC catalyst is partly dissolved and adsorbed by the negatively charged tetrahedral aluminum in the geopolymer. However, because vanadates formed under alkaline conditions are negatively charged and readily soluble in water, vanadium in the SFCC catalyst is neither immobilized by the charge balance nor effectively immobilized by physical encapsulation. Therefore, the use of SFCC catalysts to prepare geopolymers will lead to vanadium contamination in the environment. In order to clarify the maximum vanadium content in SFCC catalysts for preparing geopolymers without environmental risk, SFCC catalysts with different vanadium levels were prepared by leaching. It was concluded that when the vanadium content in the SFCC catalyst is lower than 1861.4 mg/kg, the vanadium content in the leachate is lower than 2 mg/L and the geopolymer can be considered a non-hazardous material.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
Journal of Materials Chemistry A
·
2021
·Vol. 9
·期 34
·18477-18487
·
IF 12.732
Menghua Yang,Xuewei Wang,Jinfeng Wu,Yue Tian,Xingyu Huang,Ping Liu,Xianyang Li,Xinru Li,Xiaoyan Liu,Hexing Li
Lithium sulfur (Li–S) batteries have been investigated as ideal candidates for future high-density energy storage systems with the advantages of abundant reserves, high energy density and competitive cost. The key issues are the severe shuttling of polysulfides and sluggish redox kinetics. Herein, we report a novel metal–organic framework-derived Co2P–ZnS/ZnS–C nanocomposite constructed from inner Co2P–ZnS and outer ZnS–C heterostructures. Both the experimental results and theoretical calculations demonstrated that these dual electrocatalytic heterostructures enabled strong affinity with polysulfides and facilitated the reaction kinetics. Meanwhile, the hollow carbon polyhedron provided fast electron/ion transfer channels and effectively buffered volume expansion during cycling. As anticipated, a high initial capacity of 1503 mA h g−1 was achieved at 0.2C with Co–Zn/Zn–C/S as a cathode, together with excellent stability after 500 cycles at 1C. Even a high reversible capacity of 540 mA h g−1 was achieved at 1C after 200 cycles under an elevated sulfur loading of 3.65 mg cm−2. This work presents a new strategy for designing dual electrocatalytic hosts for immobilization and conversion of polysulfides, which may offer more opportunities as cathodes in stable Li–S batteries with high energy density.
研究领域:
材料科学
·
能源存储技术
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DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Xia Hua,Zhihong Gao,Qianqian Wang,Wenming Hao,Xiaoliang Yan,Ruifeng Li
The accumulation of heavy metal on catalysts during petrochemical process produces deactivated catalysts that cannot be directly disposed in nature, due to the high toxicity. The elimination of the detrimental effect of heavy metal ions can be achieved by immobilizing these ions in the form of spinel structure, yet this requires high-temperature calcination . Herein, Ni 2+ ions are stabilized and confined within the surface NiAl 2 O 4 on Al 2 O 3 through hydrothermal process and subsequent low-temperature thermal treatment. The formation of nickel aluminum layered double hydroxide (NiAl-LDH) as intermediate favors the generation of surface aluminate spinel at the temperature as low as 450 °C. The formation of surface spinel is beneficial to restricting the mobility of Ni 2+ ions. The growth and immobilization mechanisms of the surface spinel structure were proposed, which contributes to alternative and promising route for the low-temperature approach of the retention of the heavy metal ions mobility.
研究领域:
环境催化
·
材料化学
·
工业化学
·
固体化学
·
污染控制
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DOI
高影响因子
危险品化学品经营许可证（带存储）