Nano Today
·
2023
·
IF 17.4
Honglei Zhu,Huayan Li,Kunjie Gao,Yi Huang,Yuru Liao,Weichu Hu,Feng Chen,Haoyuan Jiang,Xiaomei Liang,Haonan Song,Yuxin Wang,Hanrong Huang,Yifan Wang,Fan Liu,Hao Huang,Qian Wang,Xiao Yi
Intrauterine adhesion (IUA) is one of the main causes of female infertility. Extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSCs), are consisted of a complete lipid bilayer structure, rich in multiple factors and miRNAs related to tissue regeneration , which are the main effective paracrine components of MSCs to treat IUA. However, the poor secretion and bioactivity of EVs leading to the unexpected therapeutic efficacy of MSC-EVs, have been obstacles to their widespread use in clinical practice. Here, we developed human chorionic villi (CV)-derived nanofibers that mimic the tissue-specific ECM microenvironment of CV-derived MSCs, maintain the stemness of MSCs for a long time, and are rich in apolipoproteins , HSP90 , ANXA1-A6, etc., which can enhance EV secretion and bioactivity. Then, the CV-nanofiber bionic culture system for mesenchymal stem cells was constructed to achieve large-scale cultivation of MSCs for continuous collection of high-concentration EVs enrichment supernatant. Additionally, EVs obtained by this system were encapsulated with CV-nanofibers to form EVs-ECM aggregate, which can effectively promote endometrium regeneration toward supporting fetal development and live births. Our study provides a new idea for enhancing EV secretion and bioactivity, and a cell-free therapy for treating IUA by EVs, thus promoting the potential of clinical application.
研究领域:
生殖医学
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干细胞生物学
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组织工程
·
再生医学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 34
·7133-7139
·
IF 4.543
Yike Fu,Tong Chen,Gang Wang,Tongxu Gu,Congkun Xie,Jie Huang,Xiang Li,Serena Best,Gaorong Han
MicroRNAs (miRNAs) play a key role in regulating gene expression but can be associated with abnormalities linked to carcinogenesis and tumor progression. Hence there is increasing interest in developing methods to detect these non-coding RNA molecules in the human circulation system. Here, a novel FRET miRNA-195 targeting biosensor, based on silica nanofibers incorporated with rare earth-doped calcium fluoride particles (CaF2:Yb,Ho@SiO2) and gold nanoparticles (AuNPs), is reported. The formation of a sandwich structure, as a result of co-hybridization of the target miRNA which is captured by oligonucleotides conjugated at the surface of CaF2:Yb,Ho@SiO2 fibers and AuNPs, brings the nanofibers and AuNPs in close proximity and triggers the FRET effect. The intensity ratio of green to red emission, I541/I650, was found to decrease linearly upon increasing the concentration of the target miRNA and this can be utilized as a standard curve for quantitative determination of miRNA concentration. This assay offers a simple and convenient method for miRNA quantification, with the potential for rapid and early clinical diagnosis of diseases such as breast cancer.
研究领域:
生物传感器
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纳米技术
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分子生物学
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癌症研究
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
Small
·
2019
·Vol. 15
·期 41
·1903016
·
IF 10.856
Nan Yan,Lin Lin,Caina Xu,Huayu Tian,Xuesi Chen
Developing tumor-responsive diagnosis and therapy strategies for tumor theranostics is still a challenge owing to their high accuracy and specificity. Herein, an AND logic gated–DNA nanodevice, based on the fluorescence nucleic acid probe and polymer-modified MnO 2 nanosheets, for glutathione (GSH)-gated miRNA-21 signal amplification and GSH-activated magnetic resonance (MR) imaging–guided chemodynamic therapy (CDT) is reported. In the presence of overexpressed miRNA and GSH (tumor cells), the nanodevice can be in situ activated and release significantly amplified fluorescence signals and MR signals. Conversely, the fluorescence signal is quenched and MR signal remains at the background level with low miRNA and GSH (normal cells), efficiently reducing the false-positive signals by more than 50%. Under the guide of miRNA profiling and MR imaging, the tumor-responsive hydroxyl radical ( · OH) can effectively kill tumor cells. Furthermore, the nanodevice shows catalase-like activity and glucose oxidase–like activity with the performance of O 2 production and glucose consumption. This is the first time to fabricate a tumor-responsive theranostic DNA nanodevice with tumor-specific signal amplification of microRNA and GSH-activated MR imaging for CDT, potential hypoxia relief and starvation therapy, which provides a new insight for designing smart theranostic strategies.
研究领域:
生物医学工程
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纳米技术
·
诊疗学
·
分子生物学
·
癌症研究
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Tingyi Huang,Li Tan,Wenyi Zhang,Ying Ma,Ziqi Lian,Benlin Hong,Jianqiang Hu
Highly sensitive and simple biomarker determination is crucial for diagnosis and treatment of early diseases. However, it remains a tremendous challenge for rapid, accurate and real time detection of biomarkers at the early stage of diseases due to its extremely low content and serious interference. Herein, we have successfully constructed an ultrasensitive electrochemiluminescence (ECL) biosensor based on 2,6-bis(2-benzimidazolyl)pyridine platinum(II) (BZIMPY-Pt) complexes with aggregation-induced electrochemiluminescence (AIECL) phenomenon, which was utilized to detect miRNA-155 at early diseases. The BZIMPY-Pt complexes with AIECL properties and had high affinity with G-quadruplex DNA compared to other square planar pyridyl Pt(II) complexes, which exhibited 13-fold enhancements in ECL intensity in the aggregated state. The constructed biosensor possessed high sensitivity, wide linear range (1 fM-10 nM), low limit of detection (∼0.22 fM), high selectivity and good stability and reproducibility for miRNA-155 detection. The ECL biosensor was successfully applied to determine miRNA-155 in the serum samples of early-stage diagnose. This AIECL-based strategy overcomes traditional limitations in low-abundance biomarker analysis through synergistic signal amplification and background suppression mechanisms, which is potential for clinical diagnosis of early diseases.
研究领域:
生物传感器
·
生物分析化学
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纳米技术
·
分子诊断学
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电化学
·
临床化学
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金属配合物药物
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Fenfang Li,Tiantian Xue,Yanteng Xu,Haixia Wang,Enguo Ju,Mingqiang Li,Yu Tao
Acute liver failure (ALF) is a life-threatening disease for which orthotopic liver transplantation remains one of the most effective treatments. Cell therapy, particularly through the transplantation of artificial bioengineered liver grafts, emerges as a promising alternative to the whole liver transplantation in ALF management. However, the limited availability of functional human hepatocytes, the constrained techniques for swiftly creating artificial bioengineered liver grafts, and the poor cellular survival post-transplantation present significant challenges for advancing clinical applications in liver regeneration. In this study, we introduce a novel approach utilizing microfluidics to create cell-laden microgels for fabricating artificial liver microtissues, in which the controlled hepatic differentiation of stem cells and the development of liver microtissues are incorporated. The cell-laden ARGH microgels (GelMA/HAMA microgels containing AuNCs-miRNA) serving as building blocks for rapid engineering tissue construction, demonstrate the capacity for the one-step promotion of hepatic differentiation of stem cells for a short period of time. Additionally, the bioengineered liver microtissues exhibit multiple biological functionalities, including scavenging ROS, modulating inflammatory environment, and promoting angiogenesis, and can maintain long-term stability after transplantation in vivo. Furthermore, our findings reveal that the in vivo transplantation of the bioengineered liver microtissues offers therapeutic benefits in two drug-induced liver failure experimental models. The transcriptome sequencing analysis further elucidates that the microtissue transplantation ameliorated liver failure by improving liver biofunctions, reducing inflammation and oxidative stress, thereby promoting liver regeneration. Overall, this study presents an effective approach leveraging functional biomaterials and microfluidic techniques to fabricate artificial liver microtissues for liver failure treatment.
研究领域:
生物医学工程
·
组织工程
·
再生医学
·
微流控技术
·
肝脏病学
·
纳米医学
·
干细胞治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Energy
·
2019
·
IF 15.548
Fu-Ting Wang,Yi-Han Wang,Jing Xu,Ke-Jing Huang,Zhen-hua Liu,Yun-fei Lu,Shu-yu Wang,Zi-wei Han
A self-powered microRNA (miRNA) biosensing device is fabricated with high-energy enzymatic biofuel cell (EBFC) and cruciform DNA (cDNA). Sulfur-selenium co-doped graphene/gold nanoparticles (S–Se-GR/AuNPs) is synthesized and used as supporting substrate of biocathode and bioanode . The glucose oxidase (GOD) molecules are bonded to carboxyl-functionalized AuNPs through the condensation reaction between the amino groups in enzyme and carboxyl groups on the AuNPs to form the bioanode. The ultra-thin porous carbon shell/AuNPs-complementary strand of cDNA (UPCS/AuNPs-cDNA) is meticulously designed. The potassium ferricyanides are then inserted in mesoporous UPCS/AuNPs as the biocathode electron acceptor , and the cruciform DNA bioconjugate is synthesized as signal amplifier. When target miRNA is added, the hybridization reaction happens between miRNA and capture probe DNA on the biocathode to open the capture probe DNA chain. Cruciform DNA bioconjugate is immobilized onto the biocathode through base pairing with the capture DNA on the biocathode, which can release electron acceptor [Fe(CN) 6 ] 3- , resulting in the dramatically increase of the open circuit voltage of the EBFCs. The self-powered biosensor responds linearly in the miRNA level range of 0.5–10000 fM with a detection limit of 1.5 × 10 −16 mol L −1 . Detection of miRNA in spiked serum samples is also realized with the self-powered biosensor, demonstrating its great potential in the clinical applications.
研究领域:
生物工程
·
生物医学工程
·
分析化学
·
分子生物学
✓ 已验证
DOI
高影响因子
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