JOURNAL OF CONTROLLED RELEASE
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2019
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IF 7.901
Wendi Teng,Liyun Zhao,Songtao Yang,Chengying Zhang,Meiyu Liu,Jie Luo,Junhua Jin,Ming Zhang,Chen Bao,Dan Li,Wei Xiong,Yuan Li,Fazheng Ren
Nonalcoholic fatty liver disease (NAFLD) is the early stage of many metabolic syndromes . The intervention of NAFLD can prevent its further development into severe metabolic syndromes. Given the inefficiency and side effects of chemical drugs for treating NAFLD, the hepatic-targeted nanocarriers loaded with bioactive compounds may offer a more effective and acceptable strategy for eliminating NAFLD. Here we developed hepatic-targeted oxidized starch-lysozyme (OSL) nanocarriers to specifically deliver resveratrol (Res) to liver tissue in order to maximize its therapeutic efficiency. The hepatic targeting was achieved using covalently conjugated galactose (Gal), which is recognized by the asialoglycoprotein receptors specifically expressed in hepatocytes. In steatotic HepG2 cell model, treatment with hepatic-targeted Gal-OSL/Res nanocarriers enhanced the cellular Res uptake and anti-lipogenesis capabilities, and effectively decreased triglyceride accumulation by modulating AMP-activated protein kinase (AMPK)/silent information regulation 2 homolog 1(SIRT1)/fatty acid synthase (FAS)/sterol regulatory element-binding protein-1c (SREBP1c) signaling pathway. In mice, Gal-OSL increased Res delivery into liver tissues and increased their hepatic effective concentration in liver. Most importantly, Gal-OSL/Res nanocarriers effectively reversed NAFLD and recovered hepatic insulin sensitivity of NAFLD mice to the healthy state. Furthermore, Gal-OSL/Res efficiently ameliorated lipid deposition and insulin resistance by modulating AMPK/SIRT1/FAS/SREBP1c signaling pathway and downregulated insulin receptor substrate-1 (IRS-1) phosphorylation at serine 307 in liver. These findings suggested that the hepatic-targeted Gal-OSL nanocarriers delivering Res could potentially serve as a safe and promising platform for NAFLD and other liver related diseases.
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FOOD RESEARCH INTERNATIONAL
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2017
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IF 3.086
Wusigale,Zheng Fang,Lyulin Hu,Yahui Gao,Juan Li,Li Liang
Folic acid is a synthetic form of the B-group vitamin known as folate and essential for a variety of physiological processes. However, it decomposes under UV irradiation, causing indirect oxidation and structural change of protein. In this study, the protective effect of resveratrol against the photodecomposition of folic acid and its caused protein structural change was investigated by using fluorescence and absorbance spectroscopy, high performance liquid chromatography and ABTS assay . It was found that resveratrol could inhibit the folic acid decomposition and control the decomposition process, depending on the polyphenol concentration and addition time. Transformation of resveratrol was accelerated by photodecomposition of folic acid. Antioxidant activity of resveratrol was important for the protective effect. Moreover, resveratrol could also inhibit the unfolding of beta-lactoglobulin caused by the folic acid decomposition by using circular dichroism .
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BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2024
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IF 3.2
Jianmin Chen,Juan Chen,Baozhu Feng,Meilian Ning,Wanhui Wu,Shiqi Zou
Gout predominantly stems from hyperuricemia, precipitating the accumulation of urate crystals and consequent joint inflammation, swelling, and pain, thereby compromising the quality of life and presenting a formidable medical dilemma. Although conventional treatments like allopurinol and febuxostat target uric acid reduction via xanthine oxidase (XO) inhibition, they often entail adverse effects, prompting the exploration of safer alternatives. Resveratrol, a polyphenolic compound abundant in fruits and vegetables, has emerged as a potential XO inhibitor. However, its precise inhibitory mechanisms remain poorly understood. This study aims to comprehensively investigate resveratrol's XO inhibition through mechanistic insights, molecular docking simulations, animal model experiments, and biochemical analysis, contributing valuable insights to the development of novel therapeutics for hyperuricemia and gout.
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FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Qiyong Jiang,Meng Liu,Zhaosheng Wang,Rentang Zhang,Yuxiao Wang,Fengjun Guo,Qing Sun
Radical mediated reaction method was chosen to prepare ferulic acid grafted pectin (FA-P) to enhance the properties of the native pectin. HPLC and FTIR results presented that ferulic acid was successfully grafted onto the pectin molecule. Among them, FA-P conjugation with a mass ratio of 0.75:1 was the most effective with the highest grafting efficiency, emulsifying activity index and emulsifying stability index. Subsequently, FA-P (0.75:1) was used to emulsify resveratrol to prepare FA-P-R emulsion. Compared to the resveratrol emulsion emulsified with native pectin (P-R), FA-P-R had a lower ζ-potential and a 57.4% reduction in mean particle diameter. Scanning electron microscopy demonstrated that the particles of FA-P-R emulsion were more rounded, with flatter and smoother surfaces, suggesting the formation of a better emulsified morphology. In addition, the encapsulation efficiency, load capacity and light stability of FA-P-R increased by 31.6%, 31.5% and 28.0%, respectively, compared to P-R. The FA-P-R samples showed the best antioxidant properties among the four emulsions of FA-P-R, P-R, FA-P and pectin for the functional coatings prepared, with the highest DPPH and ABTS + radical scavenging activity. Finally, the FA-P-R functional coating for wrapping frozen limes was able to significantly inhibit the yellowing of frozen limes during freezing storage. The results highlight that the prepared FA-P-R functional coating can be used to inhibit the quality deterioration of frozen fruits and extend the freezing period.
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Molecular Medicine Reports
·
2024
·Vol. 29
·期 5
·1-14
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IF 3.4
Xinya Yu,Xiaoxi Li,Yunchun Xu,Yuwei Li,Ye Zhou,Junhua Zhang,Le Guo
Ulcerative colitis (UC) is a chronic idiopathic inflammatory condition affecting the rectum and colon. Inflammation and compromisation of the intestinal mucosal barrier are key in UC pathogenesis. Resveratrol (Res) is a naturally occurring polyphenol that exhibits anti‑inflammatory and antioxidant properties. Nuclear factor erythroid‑2‑related factor 2/heme oxygenase 1 (Nrf2/HO‑1) pathway regulates occurrence and development of numerous types of diseases through anti‑inflammatory and antioxidant activity. However, it is not clear whether Nrf2/HO‑1 pathway is involved in the treatment of Res in UC. Therefore, the present study aimed to investigate whether Res modulates the Nrf2/HO‑1 signaling pathway to attenuate UC in mice. Dextran sulfate sodium (DSS) was used to induce experimental UC in male C57BL/6J mice. Disease activity index (DAI) and hematoxylin eosin (H&E) staning was used to assessed the magnitude of colonic lesions in UC mice. ELISA) was utilized to quantify inflammatory cytokines (IL‑6, IL‑1β, TNF‑α and IL‑10) in serum and colon tissues. Immunohistochemistry and Western blot were used to evaluate the expression levels of tight junction (TJ) proteins [zonula occludens (ZO)‑1 and Occludin] in colon tissues. Pharmacokinetic (PK) parameters of Res were derived from TCMSP database. Networkpharmacology was employed to identify the biological function and pharmacological mechanism of Res in the process of relieving UC, and the key target was screened. The binding ability of Res and key target was verified by molecular docking. Finally, the effectiveness of key target was substantiated by Western blot. Res decreased DAI, ameliorated histopathological changes such as crypt loss, disappeatance of the mucosal epithelium, and inflammatory infiltration in mice. Additionally, Res decreased expression of pro‑inflammatory cytokines IL‑6, IL‑1β and TNF‑α and increased anti‑inflammatory factor IL‑10 expression. Res also restored the decreased protein expression of ZO‑1 and occludin after DSS treatment, increasing the integrity of the intestinal mucosal barrier. The PK properties of Res suggested that Res possesses the therapeutic potential for oral administration. Network pharmacology revealed that Res alleviated UC through anti‑inflammatory and antioxidant pathways, and confirmed that Nrf2 has a high binding affinity with Res and is a key target of Res against UC. Western blotting demonstrated that Res treatment increased the protein levels of Nrf2 and HO‑1. In conclusion, Res treatment activated the Nrf2/HO‑1 pathway to decrease clinical symptoms, inflammatory responses, and intestinal mucosal barrier damage in experimental UC mice.
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Journal of Translational Medicine
·
2024
·Vol. 22
·期 1
·1-19
·
IF 6.1
Liu Nannan,Fan Xuefei,Shao Yubao,Chen Suhuan,Wang Taorong,Yao Tao,Chen Xiaoyu
Background and purposeInterstitial lung disease (ILD) represents a significant complication of rheumatoid arthritis (RA) that lacks effective treatment options. This study aimed to investigate the intrinsic mechanism by which resveratrol attenuates rheumatoid arthritis complicated with interstitial lung disease through the AKT/TMEM175 pathway.Methods We established an arthritis model by combining chicken type II collagen and complete Freund’s adjuvant. Resveratrol treatment was administered via tube feeding for 10 days. Pathological changes in both the joints and lungs were evaluated using HE and Masson staining techniques. Protein expression of TGF-β1, AKT, and TMEM175 was examined in lung tissue. MRC-5 cells were stimulated using IL-1β in combination with TGF-β1 as an in vitro model of RA-ILD, and agonists of AKT, metabolic inhibitors, and SiRNA of TMEM175 were used to explore the regulation and mechanism of action of resveratrol RA-ILD.Results Resveratrol mitigates fibrosis in rheumatoid arthritis-associated interstitial lung disease and reduces oxidative stress and inflammation in RA-ILD. Furthermore, resveratrol restored cellular autophagy. When combined with the in vitro model, it was further demonstrated that resveratrol could suppress TGF-β1 expression, and reduce AKT metamorphic activation, consequently inhibiting the opening of AKT/MEM175 ion channels. This, in turn, lowers lysosomal pH and enhances the fusion of autophagosomes with lysosomes, ultimately ameliorating the progression of RA-ILD.Conclusion In this study, we demonstrated that resveratrol restores autophagic flux through the AKT/MEM175 pathway to attenuate inflammation as well as fibrosis in RA-ILD by combining in vivo and in vitro experiments. It further provides a theoretical basis for the selection of therapeutic targets for RA-ILD.Graphical Abstract
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MACROMOLECULAR CHEMISTRY AND PHYSICS
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2024
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IF 2.5
Min Zhong,Ren Yin,Zichao Sun,Tianjia Jiang,Weichen Sheng,Kan Zhang
Resveratrol and 2-thiophenemethylamine have been employed in the synthesis of a novel tri-functional benzoxazine (RES-th) to develop the bio-benzoxazine monomer. The chemical structure of the synthesized monomer is confirmed by various characterization technics. The polymerization behavior is monitored by in situ Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). The in situ FTIR results reveal distinct reaction mechanisms for the three oxazine rings presented in RES-th, with both ether and phenolic Mannich bridge structures observed in the products. The activation energy values of RES-th are calculated to be 119.05, 120.97, and 119.44 kJ mol −1 by Kissinger, Ozawa, and Starink methods, respectively, which are all based on the heat flow curves at various heating temperatures. The thermal stability and flame retardancy of the resulting polybenzoxazine (poly(RES-th)) are investigated by thermogravimetric analysis (TGA) and microscale combustion calorimeter (MCC). The values of T d5 and T d10 of polybenzoxazine are found to be 356 °C and 399 °C, respectively, with a char yield of 66.3% at 800 °C. The prepared polybenzoxazine also demonstrates nonflammability characteristics with the values of heat release capacity (HRC) and total heat release rate (THR) of 18.65 J (g K) −1 and 2.69 kJ g −1 , respectively. These findings suggest that the thermoset, poly(RES-th), is a promising candidate for fire-resistant applications.
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JOURNAL OF MOLECULAR STRUCTURE
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2025
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IF 4.7
Lei Wang,Chaofan Sun
Exploring the impact of photoisomerization on the antioxidant activity of compounds is of great significance in fields such as medicine and daily chemical industry. Herein, the influence of photoisomerization on the antioxidant properties of resveratrol (RES) upon photoexcitation in different solvents was systematically investigated via 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging experiments and quantum chemistry computational methods. The frontier molecular orbitals and global descriptive parameters calculated using density functional theory (DFT) and time-dependent DFT (TD-DFT) methods indicate that trans-RES exhibits higher reactivity in the S 0 state, while cis-RES shows enhanced reactivity in polar solvents under the S 1 state. Natural population analysis (NPA) charges and spin density distribution further confirm that cis-RES and trans-RES exhibit more reactive sites in the S 1 state. In addition, both theoretical and experimental results demonstrate that photoexcitation and increased solvent polarity significantly enhance the antioxidant capacity of RES. These findings highlight the modulation of antioxidant capacity by molecular configuration and environmental factors, providing fresh insights for the design and optimization of antioxidant compounds.
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RSC Advances
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2025
·Vol. 15
·期 25
·19786-19801
·
IF 4.6
Zhijie Liang,Jinzhuai Li,Shuying Luo,Shaorong Li,Kun Zhao,Hongmian Jiang,Yisi Ou,Juan Zhong,Lifeng Luo,Huali Huang,Yingying Li
Resveratrol (RSV), a natural polyphenol with potent antioxidant and anti-inflammatory properties, exhibits significant therapeutic potential for non-alcoholic fatty liver disease (NAFLD) by modulating lipid metabolism, oxidative stress, and inflammatory pathways. Despite its multi-target mechanisms and enhancement of reverse cholesterol transport, clinical translation remains limited by poor bioavailability and inefficient intracellular delivery. Conventional oral administration fails to achieve therapeutic plasma concentrations owing to extensive first-pass metabolism and low solubility. To address these limitations, this study developed galactose (Gal)-modified lipid nanoparticles (Gal-LNPs) to enhance hepatic targeting via asialoglycoprotein receptor-mediated endocytosis. These Gal-LNPs demonstrated significantly improved intracellular RSV delivery (3.49-fold uptake vs. unmodified LNPs). In NAFLD mouse models, Gal-LNP-RSV reduced hepatic lipid accumulation and serum alanine aminotransferase/aspartate aminotransferase levels by 48.3% and 58.7%/49.3%, respectively, outperforming free RSV in both aspects. These findings underscore the potential of Gal-LNPs as a transformative means of overcoming RSV's pharmacokinetic barriers, enabling the precise activation of intracellular targets while restoring metabolic and redox homeostasis. This work provides a robust framework for developing targeted nanotherapeutics against NAFLD, bridging the divide between preclinical efficacy and clinical application.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yiyang Chai,Ying Zhou,Kai Zhang,Ping Shao
Resveratrol (RES) is an important functional substance with multiple active properties. However, RES is susceptible to natural environmental conditions that reduce its bioactivity. To improve the bioavailability of RES, in this study, Catechin and Fe 3+ /Ca 2+ were selected to form supramolecules, which were then coated on the surface of hydrophobic RES nanoparticles (RES NPs) to create composite RES NPs. The obtained composite RES NPs demonstrated higher antioxidant capacity and better photo-thermal stability than RES NPs. Additionally, a pectin (PE) dietary gel was designed as a delivery carrier for RES. The results showed that the incorporation of composite RES NPs not only endowed the gels with significant dietary activity but also enhanced the texture, water retention capacity and hydrophobicity. After 28 days of storage, the retention rate of RES could be maintained above 90 % in the dietary gels. Meanwhile, the controlled release of RES was achieved in in vitro simulated digestion.
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Biomed Research International
·
2022
·
IF 3.246
Jie Jiang,Zhenghua Liu,Xiuping Zhou,Fenglan Peng,Zhihui Wang,Feng Li,Ming Li
To investigate the effect and mechanism of resveratrol on the biological behavior of cervical cancer cells (HeLa cells), the apoptosis, migration, and invasion of HeLa cells were detected by flow cytometry, wound healing, and transwell assays. The expression levels of Hedgehog signal pathway proteins (smoothened (SMO), zinc finger transcription factors (Gli1), and sonic hedgehog homolog (Shh)) were detected by quantitative real-time PCR (qPCR) and western blotting. Compared with that control group, resveratrol (RES) significantly induced apoptosis, inhibited the migration and invasion of the HeLa cells. The expression of SMO, Gli1, and Shh were downregulated in the HeLa cells treated with RES. The Hedgehog agonist purmorphamine (PUR) reversed the RES-induced increase of apoptosis and reduction of migration and invasion in the HeLa cells. In conclusion, RES induced the apoptosis and suppressed the migration and invasion of HeLa cells by inhibiting Hedgehog signal pathway.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Lin Bai,Rundong Liu,Ruike Wang,Yongjuan Xin,Zuntao Wu,Yue Ba,Huizhen Zhang,Xuemin Cheng,Guoyu Zhou,Hui Huang
This study examined the neuroprotective properties of resveratrol (Res) and its target sirtuin1 (SIRT1) against lead (Pb)-mediated toxicity and discovered that both resveratrol treatment and SIRT1 overexpression restored blocked autophagic flux as well as reduced β-amyloid (Aβ) contents. Four-week-old male C57BL/6 mice were employed to consumed 0.2% Pb(Ac) 2 solution or deionized water for 3 months followed by 12 months of Res (50 mg/kg BW) or vehicle gavage. In in vitro study, SH-SY5Y cells were pretreated with the SIRT1 activator SRT1720 (2 μM) or the inhibitor EX527 (2 μM) for 2 h, then 25 μM of Pb(Ac) 2 was added and incubated for 48 h. Western blotting, RT-qPCR, enzyme-linked immunosorbent assay (ELISA), and Lyso-Tracker Red Staining were next used to estimate the potential alterations of the autophagic pathway as well as BACE1-mediated amyloid processing in response to Pb exposure, respectively. Our data revealed that Res treatment or SIRT1 activation resisted the induction of autophagy by Pb exposure through inhibition of LC3 and Beclin-1 expression and promoted the degradation of Aβ and Tau phosphorylation. Besides, the SIRT1 activator (SRT1720) downregulated the expression of BACE1, the rate-limiting enzyme for Aβ production, by inhibiting the activation of nuclear factor-κB (NF-κB) in Pb-treated SH-SY5Y cells, which resulted in reduced Aβ production. Collectively, we verified the role of Res-SIRT1-autophagy as well as the SIRT1-NF-κB-BACE1 pathway in Pb-induced neuronal cell injury by in vivo or in vitro models. Our findings further elucidate the important role of SIRT1 and Res in counteracting Pb neurotoxicity, which may provide new interventions and targets for the subsequent treatment of neurodegenerative diseases.
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