ACS Central Science
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2024
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IF 12.7
Yujie Wang,Zhengao Di,Minglang Qin,Shenming Qu,Wenbo Zhong,Lingfeng Yuan,Jing Zhang,Julian M. Hibberd,Ziyi Yu
In this study, an innovative approach is presented in the field of engineered plant living materials (EPLMs), leveraging a sophisticated interplay between synthetic biology and engineering. We detail a 3D bioprinting technique for the precise spatial patterning and genetic transformation of the tobacco BY-2 cell line within custom-engineered granular hydrogel scaffolds. Our methodology involves the integration of biocompatible hydrogel microparticles (HMPs) primed for 3D bioprinting with Agrobacterium tumefaciens capable of plant cell transfection, serving as the backbone for the simultaneous growth and transformation of tobacco BY-2 cells. This system facilitates the concurrent growth and genetic modification of tobacco BY-2 cells within our specially designed scaffolds. These scaffolds enable the cells to develop into predefined patterns while remaining conducive to the uptake of exogenous DNA. We showcase the versatility of this technology by fabricating EPLMs with unique structural and functional properties, exemplified by EPLMs exhibiting distinct pigmentation patterns. These patterns are achieved through the integration of the betalain biosynthetic pathway into tobacco BY-2 cells. Overall, our study represents a groundbreaking shift in the convergence of materials science and plant synthetic biology, offering promising avenues for the evolution of sustainable, adaptive, and responsive living material systems.
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高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Wang Mengting,Mao Haiguang,Ke Zhijian,Wang Jinbo
Background: The hypoglycemic effects of Chinese bayberry leaves proanthocyanidins (BLPs) have been demonstrated. It is unclear, nevertheless, whether BLPs reduced postprandial blood glucose levels by regulating glucose uptake and glucose transport.
Method: This study investigated the effect of BLPs (25, 50 and 100 μg/mL) on glucose uptake and glucose transport in human intestinal epithelial cells (Caco-2 cells). The uptake of 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2-NBDG) and disaccharidases activity in Caco-2 cells were measured. The glucose transport ability across the cell membrane was determined using the established Caco-2 monolayer model. The transcript and protein levels of key glucose transporters were analyzed using real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting, respectively.
Results: The results showed that BLPs significantly decreased glucose uptake and disaccharidases activity (p<0.05). Otherwise, BLPs treatment obviously inhibited glucose transport across the Caco-2 monolayer in both simulated-fast (5 mM glucose) and simulated-fed (25 mM glucose) conditions. It was attributed to the suppression of glucose transporter2 (GLUT2) and sodium-dependent glucose cotransporter 1 (SGLT1) by BLPs. BLPs were found to significantly downregulated the transcript level and protein expression of glucose transporters (p<0.05). Meanwhile, the mRNA expression of phospholipase C (PLC) and protein kinase C (PKC) involved in the signaling pathway associated with glucose transport were decreased by BLPs.
Conclusion: These results suggested that BLPs inhibited intestinal glucose transport via inhibiting the expression of glucose transporters. It indicated that BLPs could be potentially used as a functional food in the diet to modulate postprandial hyperglycemia.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
·
IF 6.2
Yujie Sha,Duo Zhang,Jiazichao Tu,Ruyue Zhang,Yijia Shao,Jimei Chen,Shaoyou Lu,Xiang Liu
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is a widely used organophosphate ester that can adversely affect animal or human health. The intestinal microbiota is critical to human health. High-dose exposure to TDCIPP can markedly affect the intestinal ecosystem of mice, but the effects of long-term exposure to lower concentrations of TDCIPP on the intestinal flora and body metabolism remain unclear. In this study, TDCIPP was administered to Sprague–Dawley rats by gavage at a dose of 13.3 mg/kg bw/day for 90 days. TDCIPP increased the relative weight of the kidneys ( P = 0.017), but had no effect on the relative weight of the heart, liver, spleen, lungs, testes, and ovaries ( P > 0.05). 16 S rRNA gene sequencing revealed that long-term TDCIPP exposure affected the diversity, relative abundance, and functions of rat gut microbes. The serum metabolomics of the rats showed that TDCIPP can disrupt the serum metabolic profiles, result in the up-regulation of 26 metabolites and down-regulation of 3 metabolites, and affect multiple metabolic pathways in rat sera. In addition, the disturbed genera and metabolites were correlated. The functions of some disturbed gut microbes were consistent with the affected metabolic pathways in the sera, and these metabolic pathways were all associated with kidney disease, suggesting that TDCIPP may cause kidney injury in rats by affecting the intestinal flora and serum metabolism.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Boliang Li,Binfei Li,Liping Du,Teng Lu,Guolin Shao,Zhaomin Li,Mengyuan Zhang,Junhao Zhang
High carbon emissions and low efficiency in thermal utilization are pivotal challenges in heavy oil development. Addressing the urgent need to enhance heavy oil production, this paper introduces an innovative approach in which aerogel and flue gas serve as supplementary additives in steam thermal recovery. To investigate the mechanisms behind the improved heat transfer and oil recovery facilitated by the combination of aerogel and flue gas, condensation heat transfer experiments and 2-D visual oil displacement experiments were carried out. The condensation heat transfer coefficient, oil displacement performance, and heat utilization efficiency were analyzed and compared. The results showed the porous structure of the aerogel enhances its thermal insulation properties by adsorbing flue gas, thereby reducing the steam-to-cold object condensation heat transfer coefficient by 60.6%. The cementation between aerogel nanoparticles (NPs) retained in the formation and rock particles formed an insulating layer that impedes the upward propagation of steam heat. Combined with efficacy of flue gas foam in improving fluid flow, this promoted the horizontal expansion of the steam chamber, resulting in an 8.2% and 3.4% increase in the steam chamber’s extent compared to using steam and a combination of steam with aerogels, respectively. Moreover, the profile control and thermal insulation impacted following the NPs, lowering the water cut in the produced fluid and enhancing the oil recovery by 7.9%. Upon the application of flue gas, this enhancement in recovery extended to 9.3%.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Kegang Wu,Tong Zhang,Xianghua Chai,Pingping Wang,Xuejuan Duan,Huiying Hu
This study investigated the antibacterial activity and mechanism of action of 2-methoxycinnamaldehyde (MCA) against Salmonella enteritidis and evaluated the efficacy of MCA as a food disinfectant. The minimum inhibitory concentration (MIC) of MCA against S. enteritidis was 0.16 mg/mL. The acquired antimicrobial resistance toward MCA was weak, with low hemolytic activity and good in vitro stability. S. enteritidis exposed to MCA exhibited the formation of both holes and grooves on the cell surface, alterations in the structure and conformation of membrane proteins, leakage of nucleic acids, production of reactive oxygen species, membrane lipid peroxidation, binding to the groove of DNA, and destruction of protein secondary structures. After treatments with 1 MIC, 2 MIC, and 4 MIC of MCA for 20 min, S. enteritidis strain in pork slices was significantly inhibited ( p < 0.05), and the reduction amounts ranged from 1.31 to 4.98 log CFU/g. Additionally, the treated pork contained low levels of residual disinfectants and demonstrated high sensory acceptability. This study provides new insights into the antibacterial mechanism of MCA, which has broad application potential as a plant-derived antibacterial washing disinfectant to prevent foodborne pathogens.
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DALTON TRANSACTIONS
·
2024
·
IF 3.5
Cengceng Du,Zhenyu Wang,Yiming Wang,Wenjuan Xu,Yuqiu Huo,Hongbin Sun,Guangwen Xu
Constructing an environmentally friendly and efficient electrocatalyst holds important and profound significance for energy-efficient hydrogen production. Replacing the oxygen evolution reaction with a lower potential urea oxidation reaction (UOR) may save energy in water electrolysis to produce hydrogen. The UOR is characterized by its high energy barrier, which results in slow reaction kinetics. In this study, we introduced Ba(OH)2 into Ni(OH)2 to form uniform nanosheets. Due to the introduction of Ba2+, the lattice expansion of Ni(OH)2 was triggered, leading to significant improvement in UOR activity. The catalyst achieved a current density of 100 mA cm−2 at only 1.316 V and exhibited remarkable stability over time. Density functional theory (DFT) calculations demonstrate that the Ba–Ni(OH)2 site significantly reduces the energy barrier for urea adsorption, intermediate steps, and desorption. This work provides a novel and environmentally friendly strategy for constructing energy-efficient and highly efficient catalysts through the doping of alkaline earth metals.
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高影响因子
MOLECULES
·
2017
·Vol. 22
·期 7
·1068
·
IF 2.861
Yalu Dong,Panpan Peng,Baoping Hu,Hui Su,Shenghua Li,Siping Pang
High-energy metal–organic frameworks (MOFs) based on nitrogen-rich ligands are an emerging class of explosives, and density is one of the positive factors that can influence the performance of energetic materials. Thus, it is important to design and synthesize high-density energetic MOFs. In the present work, hydrothermal reactions of Cu(II) with the rigid polynitro heterocyclic ligands 5,5′-dinitro-2H,2′H-3,3′-bi-1,2,4-triazole (DNBT) and 5,5′-dinitro-3,3′-bis-1,2,4-triazole-1-diol (DNBTO) gave two high-density MOFs: [Cu(DNBT)(ATRZ)3]n(1) and [Cu(DNBTO)(ATRZ)2(H2O)2]n(2), where ATRZ represents 4,4′-azo-1,2,4-triazole. The structures were characterized by infrared spectroscopy, elemental analysis, ultraviolet-visible (UV) absorption spectroscopy and single-crystal X-ray diffraction. Their thermal stabilities were also determined by thermogravimetric/differential scanning calorimetry analysis (TG/DSC). The results revealed that complex1has a two-dimensional porous framework that possesses the most stable chair conformations (like cyclohexane), whereas complex2has a one-dimensional polymeric structure. Compared with previously reported MOFs based on copper ions, the complexes have higher density (ρ = 1.93 g cm−3for complex1and ρ = 1.96 g cm−3for complex2) and high thermal stability (decomposition temperatures of 323 °C for complex1and 333.3 °C for complex2), especially because of the introduction of an N–O bond in complex2. We anticipate that these two complexes would be potential high-energy density materials.Keywords:energetic materials;metal organic frameworks;densities;polynitro heterocyclic ligands
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JOURNAL OF APPLIED POLYMER SCIENCE
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2016
·Vol. 133
·期 31
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IF 1.866
Wu Kuang,Zhongnan Liu,Guodong Kang,Dandan Liu,Meiqing Zhou,Yiming Cao
The poly(2-hydroxyethyl methacrylate) grafted titanium dioxide nanoparticles were synthesized and added to the substrate of flat-sheet thin film composite forward osmosis (TFC-FO) membranes. The hydrophilicity of substrate was improved, which was advantageous to enhance the water flux of TFC-FO membranes. The membranes containing a 3 wt % TiO 2 -PHEMA in the substrate exhibited a finger-like structure combined with sponge-like structure, while those with lower or without TiO 2 -PHEMA content showed fully finger-like structures. As for FO performance, the TFC-FO membranes with 3 wt % TiO 2 -PHEMA content achieved the highest water flux of 42.8 LMH and 24.2 LMH against the DI water using 2 M NaCl as the draw solution tested under the active layer against draw solution (AL-DS) mode and active layer against feed solution (AL-FS) mode, respectively. It was proven that the hydrophilic property of membrane substrates was a strong factor influencing the water flux in FO tests. Furthermore, the structural parameter was remarkably decreased with an increase of TiO 2 -PHEMA content in membrane substrate, indicating the reducing of internal concentration polarization. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43719.
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Polymer Chemistry
·
2016
·Vol. 7
·期 20
·3423-3433
·
IF 5.687
Ke Wang,Zefeng Song,Chonggao Liu,Wangqing Zhang
The thermo- and pH/CO2-responsive poly[N-[2-(dialkylamino)ethyl]acrylamide]s containing a polyacrylamide backbone but different N-substitutes of dialkylamine were synthesized and their solution properties were comparatively checked. A controllable RAFT synthesis of poly[N-[2-(dialkylamino)ethyl]acrylamide]s was achieved when a typical trithiocarbonate containing an easily cleavable R group was employed. The RAFT polymerization rate decreases with the increasing C-number in the N-substitutes. The thermo- and pH/CO2-responsive property of poly[N-[2-(dialkylamino)ethyl]acrylamide]s is firmly correlative to the N-substitutes. With the C-number in the R-substitute increasing, the solution properties of poly(N-[2-(dialkylamino)ethyl]acrylamide)s undergo a soluble-to-thermoresponsive-to-insoluble evolution, and the critical pH of poly[N-[2-(dialkylamino)ethyl]acrylamide]s gradually decreases. The dialkylamine moieties in the poly[N-[2-(dialkylamino)ethyl]acrylamide]s lead to a characteristic CO2-response during CO2/N2 bubbling. The present study reveals the structure-dependent solution properties of the thermo- and pH/CO2-responsive poly[N-[2-(dialkylamino)ethyl]acrylamide]s, and these multistimuli-responsive polymers are believed to be very useful due to the controllable RAFT synthesis and tunable solution properties.
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GENE
·
2016
·
IF 2.319
Jing Qian,Chunhua Ren,Jianjun Xia,Ting Chen,Zonghe Yu,Chaoqun Hu
The non-specific protease inhibitor alpha-2-macroglobulin (A 2 M) is a key macromolecular glycoprotein that involved in host immune defense against pathogens in vertebrates and invertebrates. However, no research regarding A 2 M has been developed in echinoderms to date. In this study, the full-length cDNA of A 2 M was cloned from the sea cucumber ( Holothuria atra ), which is a tropical species widely distributed along the coasts of the South China Sea and designated HaA 2 M . HaA 2 M possesses all three conserved functional domains of known A 2 M proteins, including the bait region domain, thioester domain and receptor-binding domain. Compared to fish and shrimp A 2 Ms, the histidine residue from the catalytical regions is well conserved in HaA 2 M. HaA 2 M mRNA was predominantly expressed in coelomocytes and, to a lesser extent, in the body wall, intestine and respiratory tree. A 2 M activity was detected in the coelomic fluids of H. atra . The mRNA expression and activity levels were investigated in the major immune tissues and coelomic fluids of H. atra after challenge with inactivated Vibrio alginolyticus or polyriboinosinic polyribocytidylic acid [Poly (I: C)]. RNA interference (RNAi)-mediated knockdown of HaA 2 M resulted in a significant reduction of HaA 2 M gene transcript level (86%). RNAi-mediated silencing of HaA 2 M gene significantly decreased the A 2 M activity (38%) and increased the number of viable bacteria (2.8-fold) in the coelomic fluids of H. atra infected by V. alginolyticus . Our study, as a whole, supplied the evidences for HaA 2 M as an immune-relevant molecule and it might have multiple functions in the innate immune system of H. atra .
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CHIRALITY
·
2017
·Vol. 29
·期 7
·340-347
·
IF 1.956
Hongxing Dong,Danxia Zhang,Hailong Lin,Yudan Wang,Lijia Liu,Meixia Zheng,Xiaobo Li,Chunhong Zhang,Junqing Li,Peng Zhang,Juhyok So
Acrylamide (AM) was copolymerized with ethylene glycol dimethacrylate (EGDMA) in the presence of ( R )-1,1′-binaphthalene-2-naphthol (BINOL) as the template molecules on the surface of silica gel by a free radical polymerization to produce a chiral stationary phase based on the surface molecularly imprinted polymer (SMIP-CSP). The SMIP-CSP showed a much better separation factor ( α = 4.28) than the CSP based on the molecularly imprinted polymer (MIP-CSP) without coating on the silica gel ( α = 1.96) during the chiral separation of BINOL enantiomers by high-performance liquid chromatography. The influence of the pretreatment temperature and the content of the template molecule (( R )-BINOL) of the SMIP-CSP, and the mobile phase composition on the separation of the racemic BINOL were systematically investigated.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 7
·1-11
·
IF 2.101
He Juan,Lu Xingping,Yu Jie,Wang Li,Song Yonghai
Abstract A novel Co(OH) 2 /glassy carbon electrode (GCE) has been fabricated via metal–organic framework (MOF)-directed method. In the strategy, the Co(BTC, 1,3,5-benzentricarboxylic acid) MOFs/GCE was firstly prepared by alternately immersing GCE in Co 2+ and BTC solution based on a layer-by-layer method. And then, the Co(OH) 2 with hierarchical flake nanostructure/GCE was constructed by immersing Co(BTC) MOFs/GCE into 0.1 M NaOH solution at room temperature. Such strategy improves the distribution of hierarchical Co(OH) 2 nanostructures on electrode surface greatly, enhances the stability of nanomaterials on the electrode surface, and increases the use efficiency of the Co(OH) 2 nanostructures. Scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray powder diffraction, energy dispersive spectroscopy, Fourier transform infrared spectroscopy, and Raman spectra were used to characterize the Co(BTC) MOFs/GCE and Co(OH) 2 /GCE. Based on the hierarchical Co(OH) 2 nanostructures/GCE, a novel and sensitive nonenzymatic glucose sensor was developed. The good performance of the resulted sensor toward the detection of glucose was ascribed to hierarchical flake nanostructures, good mechanical stability, excellent distribution, and large specific surface area of Co(OH) 2 nanostructures. The proposed preparation method is simple, efficient, and cheap . Graphical Abstract
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