JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 12
·8786-8794
·
IF 2.993
Wang Xiaoqiang,Xu Baomei,Liu Zhenzhen
Supramolecular anchoring of metalloporphyrins in a protein is an attractive approach to the generation of artificial enzymes. Here, we employ the hydrophobic nanocage of single-ring mutant of bacterial GroEL protein for this purpose. We found that multiple monomeric hemin cofactors can be efficiently loaded into the protein nanocage. The as-prepared biohybrid possessed an oxidase-like catalytic activity and followed the typical Michaelis–Menten kinetics and a ping-pong mechanism in the H 2 O 2 -mediated oxidation of model substrates. In comparison with natural peroxidase, the artificial enzyme exhibited higher affinity for the model substrate. A simple and sensitive colorimetric method for the quantitative detection of H 2 O 2 and glucose was also developed based on the artificial enzyme, with the detection limits determined to be 3.0 μM for H 2 O 2 and 5.0 μM for glucose, respectively. The protein nanocage-based artificial enzyme is very flexible and is envisioned to be adapted readily for binding other metal complexes and catalysis of other reactions.
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CELL STRESS & CHAPERONES
·
2023
·Vol. 28
·期 4
·395-407
·
IF 3.8
Wu Yi,Min Luyao,Zhang Peng,Zhang Lin,Xu Yuting,Li Danhua,Zheng Maojin,Pei Dongsheng,Wang Qingling
ORP5 is a transmembrane protein anchored to the endoplasmic reticulum, which mainly functions as a lipid transporter and has reportedly been linked to cancer. However, the specific mechanism of ORP5 action in cervical cancer (CC) is unclear. In this study, we found that ORP5 promotes the migration and invasive ability of CC cells in vitro and in vivo. In addition, ORP5 expression was linked to endoplasmic reticulum stress, and ORP5 encouraged CC metastasis by inhibiting endoplasmic reticulum stress. Mechanistically, ORP5 inhibited endoplasmic reticulum stress in CC cells by stimulating ubiquitination and proteasomal degradation of SREBP1 to reduce its expression. In conclusion, ORP5 promotes the malignant progression of CC by inhibiting endoplasmic reticulum stress, providing a therapeutic target and strategy for CC treatment.
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ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 6
·1453-1460
·
IF 6.15
Wang Yu-zhe,Yang De-hua,Wang Ming-wei
Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are regarded as ‘incretins’ working closely to regulate glucose homeostasis. Unimolecular dual and triple agonists of GLP-1R and GIPR have shown remarkable clinical benefits in treating type 2 diabetes. However, their pharmacological characterization is usually carried out in a single receptor-expressing system. In the present study we constructed a co-expression system of both GLP-1R and GIPR to study the signaling profiles elicited by mono, dual and triple agonists. We show that when the two receptors were co-expressed in HEK 293T cells with comparable receptor ratio to pancreatic cancer cells, GIP predominately induced cAMP accumulation while GLP-1 was biased towards β-arrestin 2 recruitment. The presence of GIPR negatively impacted GLP-1R-mediated cAMP and β-arrestin 2 responses. While sharing some common modulating features, dual agonists (peptide 19 and LY3298176) and a triple agonist displayed differentiated signaling profiles as well as negative impact on the heteromerization that may help interpret their superior clinical efficacies.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2019
·Vol. 37
·期 12
·1224-1233
·
IF 2.804
Gong Heng-Ye,Chen Yan-Gui,Yu Xing-Su,Xiao Hong,Xiao Jin-Peng,Wang Yong,Shuai Xin-Tao
Drug-resistance and drastic side effects are two major issues of traditional chemotherapy which may result in trail failure even death. Nanoparticle-mediated multidrug combination treatment has been proven to be a feasible strategy to overcome these challenges. In the present study, amphipathic block polymer of methoxyl poly(ethylene glycol)-poly(aspartyl(dibutylethylenediamine)- co -phenylalanine) (mPEG-P(Asp(DBA)- co -Phe)) was synthesized and self-assembled into pH-responsive polymeric vesicle. The vesicle was utilized to co-deliver cancer-associated epidermal growth factor (EGFR) inhibitor of afatinib and DNA-damaging chemotherapeutic doxorubicin hydrochloride (DOX) for enhanced non-small-cell lung cancer (NSCLC) therapy. As evaluated in vitro , the pH-responsive design of nanovesicle resulted in a rapid release of encapsulated drugs into tumor cells and caused enhanced cell apoptosis. In addition, in vivo therapeutic studies were conducted and the results evidenced that the co-delevery of DOX and afatinib using pH-sensitive nanovector was a promising strategy for NSCLC treatment.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 20
·5161-5168
·
IF 4.157
Zhang Xiao-Ping,Lin Bo,Shu Yang,Wang Jian-Hua
Zinc ion (Zn 2+ ) is a necessary transition metal ion in the human body and plays a vital role in biological systems. Therefore, it is meaningful for rapid, sensitive, and specific detection of Zn 2+ . Herein, a “switch-on” fluorescence sensing platform for the detection of Zn 2+ was successfully established. In this work, we report an optical nanoprobe with amino functional groups based on porphyrin metal-organic frameworks (PCN-NH 2 ) through a simple treatment method. The modification of the salicylaldehyde (SA) through the C=N acts as a quencher of PCN-NH 2 and the ligand of Zn 2+ can effectively inhibit the fluorescence emission of PCN-NH 2 . The complex of porphyrin-based metal-organic framework and salicylaldehyde (PCN/SA) acts as the fluorescence sensing probe. The presence of Zn 2+ results in the new emission peak at 448 nm due to the formation of a complex between Zn 2+ and SA. The corresponding fluorescence signal changes were used to accurately detect the concentration of Zn 2+ . The fluorescence sensing platform shows advantages, including rapid response, high sensitivity, and excellent specificity with a linear detection range of Zn 2+ between 0.1 and 250 μM, and a low detection limit (LOD) of 0.07 μM. And the feasibility of the method was verified for the quantification of Zn 2+ in cell lysate, environmental water, and plasma. Graphical abstract
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Nature Communications
·
2026
·
IF 18.1
Lu Junna,Xie Xiaochun,Yang Chao,Zhang Yidan,Zhao Xin,Lu Huanfen,Sun Madi,Chen Fangman,Wang Yingshuai,Shao Dan,Leong Kam W.,Shen He
Given the broad therapeutic benefits of molecular hydrogen, gastrointestinal delivery of this gasotransmitter is attractive for treating localized inflammatory diseases, yet conventional H 2 -releasing formulations suffer from limited solubility and poor tunability. Here, we develop gas-releasing frameworks (GRFs) for effective H 2 delivery to the gastrointestinal tract. GRFs provide 20,000-fold higher hydrogen storage than hydrogen-rich water (HRW), prevent burst leakage in acidic gastric fluid, and enable sustained, inflammation-targeted H 2 release in the intestine. After H 2 release, the dynamic framework sequesters toxic boron within a reconstituted Si-O-B architecture, promoting fecal rather than systemic boron accumulation and improving long-term biosafety. Consequently, GRFs outperform HRW in mitigating oxidative damage and inflammation in dextran sulfate sodium-induced colitis, radiation-induced colitis, and high-fat diet-induced non-alcoholic fatty liver disease. Collectively, these gas-releasing frameworks provide a feasible, safe, and translatable strategy for gastrointestinal delivery of therapeutic gases and treatment of inflammatory disorders.
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JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 4.4
Ullah Najiy,Sun Jiaxiao,Duan Mengqi,Deng Haoyu,Ning Jinli,Zheng Haotian,Yan Yongjie
Flexible carbon nanotube fiber-based neural electrodes offer significant advantages for neural interface applications; however, their practical implementation is limited by relatively low electrical conductivity, insufficient mechanical strength, and challenges in implantation. In this study, we present a synergistic strategy to address these inherent challenges through structural densification combined with metal nanoparticle integration. Continuous CNTF ribbons were fabricated by the floating chemical vapor deposition method and subsequently transformed into twisted fibers (T-CNTF) to reduce inter-tube voids and examine the mechanical response. Gold nanoparticles (Au NPs) were then electrochemically deposited onto the surfaces of T-CNTF, yielding metal-enhanced fibers (T-CNTF-Au) with improved electrical conductivity, achieving a maximum value of 2.90 × 10 5 S/m at a nominal Au deposition thickness of 100 nm. A systematic investigation demonstrated a strong structure–property relationship, where a sample prepared at a nominal Au deposition thickness of 50 nm increased fracture elongation to approximately 28%. The fabricated electrode maintained electrical continuity during 5000 bending cycles with relative resistance change below 11%. Electrochemical impedance spectroscopy and cyclic voltammetry revealed mixed capacitive-resistance interfacial behavior and repeatable voltammetric response across cycles 22–25, during which cathodic charge-storage capacity declined from approximately 95.5 to 81 mC cm⁻ 2 . The optimized fibers were integrated into multi-channel neural electrode arrays and implanted into the hippocampal region of mice using stereotaxic techniques. Electrophysiological recordings were acquired over a 2-h acute session, from which a continuous 41.7-min epoch was quantitatively analyzed using four validated contacts. The resulting median impedance magnitude at 1 kHz, spike-free RMS noise, negative-event amplitude, and signal-to-noise ratio were 13.13 kΩ, 6.45 μV, 45.97 μV, and 6.86 (16.73 dB), respectively. Overall, this study demonstrates that metal-enhanced CNTF electrodes, combining high electrical conductivity with mechanical flexibility, constitute a promising platform for next-generation neural interfaces. The proposed approach provides valuable insights into the rational design of high-performance, flexible neural electrodes for advanced neuroscience applications.
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BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2015
·Vol. 19
·期 6
·965-972
·
IF 1.113
Li Naiqiang,Chou Howard,Yu Lijun,Xu Yan
Cadaverine, as a biogenic amine, is an important platform chemical for the production of industrial polymers, such as polyamides, polyurethanes, and nylon. Previous efforts focused on the bio-based production of cadaverine using two lysine decarboxylases of Escherichia coli CadA and LdcC. In this study, we report the biotransformation of cadaverine using a lysine decarboxylase from Klebsiella oxytoca . Codon optimization of the gene encoding this enzyme was carried on for the heterologous expression in E. coli , which led to a system that converted more than 24% lysine-HCl to cadaverine compared to the same system expressing CadA. The system was further optimized by using three different inducible promoters to control the expression of lysine decarboxylase gene of K. oxytoca in E. coli . The final optimized system converted lysine-HCl to cadaverine at a conversion rate of 0.133%/min/g. When the optimized system described above is coupled to an industrial process, the combined process has the potential to produce cadaverine with high conversion efficiency (46%) from sugar.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2014
·Vol. 16
·期 4
·1-8
·
IF 2.278
Wu Yonghui,Chang Guanxiao,Zhao Yanbao,Zhang Yu
Hierarchical Fe 3 O 4 @Ni
x
SiO
y
microspheres (MSs) are synthesized via a facile hydrothermal method, using Fe 3 O 4 @SiO 2 MSs as template. The prepared Fe 3 O 4 cores present spherical shape and have a mean diameter of 500 nm, and the Ni
x
SiO
y
shell with thickness of 50 nm was composed of a large number of sheets. The Fe 3 O 4 @Ni
x
SiO
y
MSs are easily manipulated by an external magnetic force. By taking advantages of the high affinity of Ni 2+ on the shell surface toward histidine-tagged (His-tagged) proteins, the Fe 3 O 4 @Ni
x
SiO
y
MSs can be applied to enrich and magnetically separate His-tagged proteins directly from the mixture of lysed cells. Research results indicated that the Fe 3 O 4 @Ni
x
SiO
y
MSs presented negligible nonspecific protein adsorption and high protein binding ability. These MSs with a high binding capacity of 14.6 mmol g −1 are much higher than that of commercial microbeads at only 10–12 mg g −1 . Their specificity and affinity toward His-tagged proteins can be remained after four times recycling. The obtained Fe 3 O 4 @Ni
x
SiO
y
MSs are especially suitable for rapid purification of His-tagged proteins.
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BMC PLANT BIOLOGY
·
2022
·Vol. 22
·期 1
·1-11
·
IF 5.26
Han Xueyuan,Wei Xiaopeng,Lu Wenjing,Wu Qiong,Mao Linchun,Luo Zisheng
Background Our previous study has demonstrated that the transcription of AchnKCS involved in suberin biosynthesis was up-regulated by exogenous abscisic acid (ABA) during the wound suberization of kiwifruit, but the regulatory mechanism has not been fully elucidated. Results Through subcellular localization analysis in this work, AchnbZIP29 and AchnMYB70 transcription factors were observed to be localized in the nucleus. Yeast one-hybrid and dual-luciferase assay proved the transcriptional activation of AchnMYB70 and transcriptional suppression of AchnbZIP29 on AchnKCS promoter. Furthermore, the transcription level of AchnMYB70 was enhanced by ABA during wound suberization of kiwifruit, but AchnbZIP29 transcription was reduced by ABA. Conclusions Therefore, it was believed that ABA enhanced the transcriptional activation of AchnMYB70 on AchnKCS by increasing AchnMYB70 expression. On the contrary, ABA relieved the inhibitory effect of AchnbZIP29 on transcription of AchnKCS by inhibiting AchnbZIP29 expression. These results gave further insight into the molecular regulatory network of ABA in wound suberization of kiwifruit.
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INTERNATIONAL ORTHOPAEDICS
·
2010
·Vol. 35
·期 10
·1561-1567
·
IF 1.825
Li Hong,Ge Yunsheng,Wu Yang,Jiang Jia,Gao Kai,Zhang Pengyun,Wu Lingxiang,Chen Shiyi
The purpose of this study was to investigate whether hydroxyapatite (HAp) coating could induce polyethylene terephthalate (PET) artificial ligament graft osseointegration in the bone tunnel. Twenty-four New Zealand white rabbits underwent artificial ligament graft transplantation in bilateral proximal tibia tunnels. One limb was implanted with HAp-coated PET graft, and the contralateral limb was implanted with non-HAp-coated PET graft as control. The rabbits were randomly sacrificed at four and eight weeks after surgery. The loads to failure of the experimental group at eight weeks were significantly higher than those of the control group ( p = 0.0057). Histologically, application of HAp coating induced new bone formation between graft and bone at eight weeks compared with the controls. Real-time polymerase chain reaction examination revealed significantly elevated messenger ribonucleic acid expression levels of osteopontin and collagen I in the grafts of the HAp group compared with the controls at four weeks ( p < 0.05). The study has shown that HAp coating on the PET artificial ligament surface has a positive effect in the induction of artificial ligament osseointegration within the bone tunnel.
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BMC BIOTECHNOLOGY
·
2019
·Vol. 19
·期 1
·1-11
·
IF 2.303
Zhang Ge,Li Shugui,Xu Yingbo,Wang Juan,Wang Fan,Xin Yuhua,Shen Zhong,Zhang Haibo,Ma Ming,Liu Haobao
Background Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environment. Results In this study, Bacillus tequilensis CAS-MEI-2-33 was isolated from cigar wrappers to produce alkaline pectinase using TS. Subsequently, the medium and fermentation conditions for the production of pectinase by B . tequilensis CAS-MEI-2-33 were optimized. The optimal fermentation period, pH of the initial fermentation medium, concentration of TS, and inoculum amount for B . tequilensis CAS-MEI-2-33 were 40 h, 40 g/L, 7.0, and 3%, respectively. Under optimal conditions, the pectinase activity was 1370 U/mL. Then, the enzymatic properties, such as the optimum pH, reaction temperature, temperature stability, and effects of metal ions, were studied. The optimal pH was determined to be 10.0, indicating that the enzyme was an alkaline pectinase. The optimal temperature was 40 °C, and pectinase activity was stable at 40 °C. The Ag + metal ions were shown to remarkably promote enzyme activity. The pectinase was partly purified by ammonium sulfate precipitation, ion exchange chromatography, and Sephacryl S-100 chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and LC-MS/MS analyses were utilized to analyze the pectinase. Conclusions This study provided a new alkaline pectinase candidate and a new strategy for the use of TS.
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