INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Baojie Zhu,Wenxia Zhang,Shaoping Li,Jing Zhao
The polysaccharides from L. barbarum L. fruits (LBPs) are a kind of complex polysaccharides, which mainly contribute to the bioactivity of L. barbarum L. fruits and riches in acidic polysaccharides fractions. And the pharmacological research showed that the immunomodulatory effect of LBPs was closely related to α -1,4-D-galactosiduronic linkages. Therefore, as one of the main active polysaccharide fractions in LBPs, acidic polysaccharides fraction should also be used for the quality control of L. barbarum L. fruits. In this study, a novel quantitative evaluation strategy of polysaccharides from L. barbarum L. fruits based on simultaneous quantification of total polysaccharides and polysaccharides with active structure characteristics was proposed to improve their performance in the medicinal and functional food area more comprehensively and effectively, and beneficial to improve the quality control of L. barbarum L. fruits. The content of total polysaccharides in L. barbarum L. fruits was determined by HPSEC-RID method combined with universal dn / dc (0.15 mL·g −1 ). Additionally, HPAEC-PAD method combined with pectinase hydrolysis and m-hydroxybiphenyl colorimetric method were developed for the determination of acidic polysaccharide components related to active structural characteristics in L. barbarum fruits. The results revealed that the trends of total polysaccharides and acidic polysaccharides in L. barbarum L. fruits did not entirely coincide, further emphasizing the necessity of simultaneously quantifying both fractions for quality control. Furthermore, the m-hydroxybiphenyl method can be used as an alternative method to determine the content of acidic polysaccharides by HPAEC-PAD combined with pectinase hydrolysis.
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Plants-Basel
·
2025
·Vol. 14
·期 7
·1126
·
IF 4.1
Haonan Li,Michael Ackah,Frank Kwarteng Amoako,Aaron Tettey Asare,Jianbin Li,Zhenjiang Wang,Qiang Lin,Changyu Qiu,Mengdi Zhao,Weiguo Zhao
Vegetative propagation of mulberry (Morus albaL.) via sapling methods, due to the ability to exponentially multiply lateral buds on stem cuttings to enhance rapid shoot formation, is crucial for sericulture industries. The sprouting of mulberry using stubbles is an emerging method for rapid and mass production of mulberry leaves, but the growth mechanisms associated with its use remain obscure. This study is the first to report how the differential stubble lengths from mulberry plants alter and modulate phytohormones and the associated mechanisms. This study seeks to evaluate the growth mechanisms by elucidating the phytohormone signature modulation in response to differential stubble lengths of 0 cm, 5 cm, 10 cm, 20 cm, and a control via targeted metabolomics analysis in mulberry leaves. The results consistently show that the use of differential stubble lengths of mulberry promoted growth, the number of buds, aboveground biomass, and branch and leaf weights by improving the net photosynthesis, transpiration rate, stomatal conductance, and intercellular CO2relative to the control. The differential stubble lengths not only caused contrasting responses in the contents of plant hormones, including salicylic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), jasmonic acid (JA), and gibberellin (GA), but also modulated higher elemental contents relative to the control. The results further reveal significant and positive correlations between the phytohormones and all growth, biomass, and photosynthetic parameters, highlighting the role of phytohormones in the sprouting and rejuvenation of mulberry stubbles. Meanwhile, the targeted metabolomics analysis identified a total of 11 differentially accumulated phytohormones in response to the differential stubble lengths, which were significantly implicated and enriched in three major pathways, including the biosynthesis of plant hormones (ko01070), metabolic pathways (ko01100), and the plant hormone signal transduction pathway (ko04575). The use of stubbles for rapid leaf production in mulberry plants is of great importance to improve early sprouting and cutting survival, as well as shortening growth and rooting time, and is highly recommended for the sericulture industries.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Shuo Wang,Hui Han,Xinying Zhang,Xiaoyun Mao,Yali Li,Zhou Si,Niuniu Song,Yamei Ren
This study investigated the effects of acid extraction, electron beam irradiation (EBI) extraction, ultrasound extraction, and combined EBI and ultrasound extraction on the yield, structure, and functional properties of pectin from spaghetti squash ( Cucurbita pepo L. subsp. pepo ) peel. The established extraction kinetics model indicated that the combined EBI and ultrasound extraction exhibited a synergistic effect, resulting in a pectin yield (13.50 %) higher than that obtained from acid extraction (6.55 %), EBI extraction (10.91 %), and ultrasound extraction (7.56 %). The pectin produced through the combined method (EUSP) demonstrated a low degree of esterification (DE) (34.77 %) and molecular weight (M w ) of 83 kDa. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses revealed that EBI and ultrasound significantly disrupted the internal structure of the pectin. Rheological analysis indicated that the combined treatment reduced the viscosity characteristics of the pectin solution. EUSP exhibited good thermodynamic properties, emulsifying characteristics, antioxidant activities, and prebiotic activity. These findings provide a theoretical basis for the combined EBI and ultrasound extraction of pectin and offer insights for the efficient utilization of spaghetti squash peel and the application of pectin in the food industry.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Huikang Yang,Yingling Miao,Lipeng Chen,Zhuoran Li,Ruimeng Yang,Xiangdong Xu,Zhaosong Liu,Li-Ming Zhang,Xinqing Jiang
Redox-responsive theranostic nanoparticles based on poly-( N -ε-carbobenzyloxy- l -lysine) (PZLL) grafted hyaluronan (HA) (HA-g-SS-PZLL) copolymers were constructed for hepatocellular carcinoma diagnosis and therapy. These hyaluronan derivatives formed nanoparticles via a self-assembly process in aqueous solution at low concentration. Theranostic nanoparticles were obtained after loading hydrophobic doxorubicin (DOX) and superparamagnetic iron oxide (SPIO) into the core of the nanoparticles via a dialysis method. Theranostic nanoparticles exhibited redox triggered DOX release behavior, and faster DOX released from theranostic nanoparticles was detected under a reducing environment compared with slow DOX release under a normal physiological environment. Confocal laser scanning microscopy (CLSM), flow cytometry and Prussian blue staining against HepG2 cells demonstrated that HA-g-SS-PZLL theranostic nanoparticles were capable of delivering DOX and SPIO into the cells. The analysis of the anticancer effect revealed that the HA-g-SS-PZLL theranostic nanoparticles shown higher cytotoxicity against HepG2 cells than DOX-loaded HA-g-PZLL nanoparticles. In vitro T 2 magnetic resonance imaging (MRI) results exhibited that theranostic nanoparticles showed a good contrast enhancement effect, and the r 2 relaxivity value was approximately 231 Fe mM − 1 s − 1 . Finally, the theranostic nanoparticles acted as nanoprobes for HepG2 tumor-bearing BALB/c mice for in vivo MRI. Therefore, HA-g-SS-PZLL copolymers have great potential as theranostic nanoparticles for tumor-targeted diagnosis and treatment.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2013
·
IF 2.454
Jianwen Bu,Yucong Yu,Guzhanuer Aisikaer,Tiejin Ying
Mature green cherry tomato fruit were harvested and treated with ultraviolet-C (UV-C) irradiation at a predetermined dose of 4.2 kJ m −2 and stored at 18 °C for 35 days. The effects of UV-C treatment on firmness, cell wall compositions (pectin and cellulose), cell wall ultra-structure, levels of ethylene production, and activities of cell wall degrading enzymes in the fruit were investigated during storage. Major genes involved in cell wall degradation ( PME 2.1, Cel 1, PG cat and Exp 1) were also checked. Fruit firmness was better maintained in UV-C treated fruit corresponding with higher contents of cellulose and acid-soluble pectin. Transmission electron microscopy indicated that UV-C irradiation retarded cell wall disassembly in the pericarp. Ethylene production was significantly inhibited by UV-C treatment. The UV-C treatment also suppressed the transcriptional expression of PME2.1 , Cel1 , PGcat and Exp1 genes, and inhibited pectin methylesterase (PME; EC 3.1.1.11), polygalacturonase (PG; EC3.2.1.15) and cellulase (EC3.2.1.4) activities during storage. These results suggested that the inhibition of ethylene production, which in turn down-regulated expression of genes encoding cell wall degrading enzymes, might be one of the possible mechanisms of UV-C delaying tomato fruit softening.
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TETRAHEDRON-ASYMMETRY
·
2014
·
IF 2.165
Rui-Jie Chen,Gao-Wei Zheng,Yan Ni,Bu-Bing Zeng,Jian-He Xu
A new NADH-dependent carbonyl reductase Rh CR capable of efficiently reducing the ε-ketoester ethyl 8-chloro-6-oxooctanoate (ECOO) to give ethyl ( S )-8-chloro-6-hydroxyoctanoate [( S )-ECHO], an important chiral precursor for the synthesis of ( R )-α-lipoic acid, was identified from Rhodococcus sp. ECU1014. Using recombinant Escherichia coli cells expressing Rh CR and glucose dehydrogenase used for the regeneration of cofactor, 440 g L −1 (2 M) of ECOO were stoichiometrically converted to ( S )-ECHO in a space–time yield of 1580 g L −1 d −1 without the external addition of any expensive cofactor.
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Journal of Essential Oil Bearing Plants
·
2024
·
IF 2.1
Runhu Xin,Miao Liang,Rui Wang,Yajian Wu,Wei Guan,Yuping Liu
To investigate the volatiles and odor-active compounds (OACs) in essential oils (EOs) from leaves, fresh fruits and seed shells of Platycladus orientalis (L.) Franco, three EOs were extracted and analyzed by hydrodistillation combined with gas chromatography-mass spectrometry (GC-MS), gas chromatography-mass spectrometry-olfactometry (GC-MS-O) and GC. The yields of three EOs were 0.22%, 0.48% and 1.94%, respectively; a total of 77 compounds were identified by comparing their mass spectra and retention index with those of the authentic standards or the related data reported in the references, and 29 odorants were determined as OACs. Among them, 58 volatile components were identified in the leaf EO, accounting for 98.19% of the total volatiles, of which 18 compounds were screened as OACs; 47 volatile components were determined in the fruit EO, representing 98.98% of the entire volatile oil, of which 12 compounds were sniffed as OACs; 49 volatiles were found in seed shell EO, covering 97.26% of the whole volatiles, of which 19 compounds were detected as OACs. OACs in three EOs were significantly different, and the odorants with higher concentrations included α-pinene, sabinene, δ-3-carene, myrcene, β-caryophyllene, α-terpinyl acetate, cedrol, etc., which could be used as indexes for optimizing extraction process parameters of POF EOs and evaluating EO quality. The in vitro antioxidant activity of three EOs was tested by DPPH assay, ABTS assay and FRAP assay.
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Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 2
·2064-2077
·
IF 2.431
Zhang Dong,Duan Xiaoliang,Wang Yuanyuan,Shang Bo,Liu Hui,Sun Hui,Wang Yuehua
The main aim of this study is to evaluate and compare the physicochemical properties, chemical composition, and in vitro antioxidant activities of rice bran oil (RBO) from five most popular japonica rice ( Oryza sativa L.) varieties planted in China. It was found that the AV and PV of RBOs were 0.45–1.18 mg KOH/g and 0.72–1.73 mmol/kg, individually. The L *, a *, and b * values of RBOs were 24.18–26.70, 1.84–2.54, and 6.35–9.01, respectively. RBOs were rich in linoleic acid (38.63–43.50%), oleic acid (34.23–41.20%), and palmitic acid (16.26–18.93%). POL + SLL (17.44–19.29%), OLL (16.06–17.67%), OOL (13.79–17.79%), and PLL (11.70–14.79%) were the predominant triacylglycerols of RBOs. RBO from different rice varieties contained abundant bioactive constituents such as tocopherols and tocotrienols (948.59–1461.90 mg/kg), squalene (2055.65–4456.79 mg/kg), γ-oryzanol (19391.15–30024.09 mg/kg), phytosterols (10632.74–13948.17 mg/kg), and polyphenols (127.55–358.84 mg/kg). Pigments such as carotenoids and chlorophylls varied from 6.47 to 14.29 mg/kg and 0.79 to 12.96 mg/kg. The DPPH, ABTS, FRAP, and ORAC value were 837.41–1055.64, 1592.38–2106.47, 244.27–557.13, and 170.16–1776.41 µmol TE/100 g, respectively. This work could provide useful information to consumers for choosing suitable vegetable oils based on their health needs.
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Materials Today Communications
·
2024
·
IF 3.7
Lingli Xu,Jingbo Chen,Jing Zhu,Tianxiang Li,Chaoyun Shi
Recently, SiO 2 has attracted increasing attention as an anode material for lithium-ion batteries (LIBs) owing to its low cost and high theoretical specific capacity. However, its inherent poor conductivity limits its practical application. To address this issue, nanostructured material design is considered an effective solution. Herein, based on the traditional ammonium tartrate template-directed sol-gel method, we use low-cost and easily available L-tartaric acid in place of D, L-tartaric acid to prepare high-yield silica nanotubes (SNTs) with a thin wall (ca. 50 nm), small diameter (ca. 150 nm), rough surface and open ends, as well as tiny spongy-like silica nanoparticles (SNPs) by adjusting the feeding amount and feeding mode of NH 4 OH solution. The electrochemical characterization results suggest that the SNTs show better cyclability and rate performance than the SNPs, which maintain a reversible capacity of 741.3 mAh g −1 after 500 cycles at a current density of 0.4 A g −1 and deliver a high rate capacity of 819.4 mAh g −1 at a high current rate of 2 A g −1 . The excellent electrochemical performance suggests that hollow one-dimensional hollow nanotubes are more efficient in improving the electrochemical performance of SiO 2 than zero-dimensional nanoparticles , and SNTs with thin tube walls and rough surfaces are demonstrated to be fascinating and promising anode materials for LIBs.
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COMPOSITES COMMUNICATIONS
·
2026
·
IF 8.6
Xiuwen Gao,Jinhui Tan,Chong Lin,Gao Pan,Huixing Li,Fangwei Qi,Xin Li,Shuping Peng,Cijun Shuai
Photo-thermoelectric-driven vanadium valence cycling enhanced ROS generation. • NIR-triggered electrical stimulation activated calcium signaling for osteogenesis. • Synergistic antitumor and antibacterial efficacy was achieved through self-enhanced chemodynamic and thermoelectric catalysis therapy. • A multifunctional scaffold achieved tumor/bacteria elimination and bone repair. The peroxidase-like activity of vanadium carbide MXene (V 2 C) nanozyme drove the valence transition from V 3+ to V 4+ , and catalyzed hydrogen peroxide to generate reactive oxygen species (ROS) for eliminating tumor and bacteria, exhibiting potential in post-resection osteosarcoma therapy. However, V 4+ could not spontaneously reduce to V 3+ , interrupting the generation of ROS. To address this, a scaffold strategy based on photo-thermoelectric driven valence cycle was proposed. Specifically, Bi 2 Te 3 @V 2 C heterojunction was constructed via electrostatic self-assembly and then incorporated into poly(L-lactic acid) scaffold using additive manufacturing technology. X-ray photoelectron spectroscopy results demonstrated that under near-infrared light irradiation, photo-thermoelectric bismuth telluride (Bi 2 Te 3 ) generated electrons via the Seebeck effect, which efficiently reduced V 4+ to V 3+ , thereby achieving vanadium ion valence cycling and enhancing ROS production. Simultaneously, the photothermal effect of Bi 2 Te 3 enhanced peroxidase-like reaction efficiency. Furthermore, Bi 2 Te 3 triggered redox reactions through thermoelectric catalysis properties, thereby elevating ROS production levels and further enhancing antitumor and antibacterial efficacy. The scaffold-generated ROS induced mitochondrial dysfunction and apoptosis in tumor cells, while also causing the leakage of bacterial intracellular substances to reduce implant-related infection risks. The experimental results showed that both its antitumor and antibacterial rates exceeded 95%. Moreover, the scaffold delivered non-invasive electrical stimulation through photo-thermoelectric conversion, which activated calcium signaling pathways to promote bone repair. This study provided a multifunctional scaffold with antitumor, antibacterial and osteogenic capabilities.
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AQUACULTURE
·
2026
·
IF 4.4
Yu-Yang Liang,Ying-Man Yu,Chen-Chen Wu,Samwel M. Limbu,Meng-Ting Liu,Fang Qiao,Mei-Ling Zhang,Zhen-Yu Du,Yuan Luo
The expansion of intensive aquaculture and shifts in feed ingredient supply and demand have driven the adoption of high-energy feeds, resulting in metabolic disorders and diminished flesh quality in farmed fish. The natural compound trimethylamine oxide (TMAO) shows great promise as a functional feed additive, such as dietary TMAO supplementation significantly reduces the abdominal fat ratio in bluntnose black bream ( Megalobrama terminalis ), and decreases crude lipid content in the liver of Nile tilapia ( Oreochromis nilotica ), yet its effects and mechanisms in carnivorous fish such as the largemouth bass ( Micropterus nigricans ) remain unclear. This study investigated the effects of TMAO at different supplementation levels on the growth performance, lipid metabolism, fish flesh quality, and targeted metabolomics of largemouth bass. A 56-day feeding trial was designed with three TMAO supplementation levels (0.1%, 0.2%, and 0.4%). A total of 360 largemouth bass (8.78 ± 2.9 g) were randomly assigned to four treatment groups, each with three replicates. TMAO supplementation at all levels had no effect on growth performance. TMAO reduced hepatic lipid deposition in largemouth bass with a concentration-dependent effect, upregulating fatty acid β-oxidation gene expression. Targeted metabolomics analysis further revealed that TMAO increased carnitine content and elevated methylation levels by increasing SAM content in the liver. Furthermore, TMAO increased fish flesh hardness, promoted muscle fiber growth and development, enhanced muscle fiber density, and upregulated gene expression in the mTOR pathway. This study elucidates that dietary TMAO supplementation promotes lipid metabolism by increasing carnitine levels in the liver, and enhances muscle hardness by promoting muscle fiber growth and development.
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Advanced Science
·
2026
·
IF 14.1
Ruilin Du,Yudi Ren,Qiaoqiao Meng,Peng Wei,Ruyu Zhu,Junjie Bao,Ye Yang,Shuo Yan,Chaorong Yue,Xueying Zhu,Shuo Cao,Chunxia Hao,Wei Sun,Yongli Song,Xihe Li,Zhimin Wu,Siqin Bao,Yanglin Chen
Efficient derivation of neural stem cells (NSCs) from human pluripotent stem cells (PSCs) is crucial in regenerative medicine. Here, we report that the combined application of the retinoic acid receptor agonist TTNPB and the GSK3β inhibitor CHIR99021 in a chemically defined medium enabled the induction of a highly advanced NSCs (ANSCs) population from PSCs. ANSCs display robust neuroectodermal gene expression and a heightened capacity for neural lineage commitment. The combination of TTNPB and CHIR99021 markedly enhanced global chromatin accessibility, particularly at neuroectoderm-specific regulatory elements such as PAX6 and SOX1 , in parallel with reduced accessibility at the loci of pluripotency factors. Notably, TTNPB alone also exerts a marked effect in enhancing chromatin accessibility. Untargeted metabolomic analysis identified a distinct neural-ectoderm associated metabolic state in ANSCs, prominently characterized by elevated choline, alongside S-(5′-adenosyl)-L-homocysteine, adenosine 5′-diphosphate, and glutathione. Exogenous addition of these metabolites was sufficient to induce neuroectodermal marker expression, highlighting the instructive role of the metabolic network in neural fate induction. Moreover, functional studies showed that ANSCs enabled engraftment into depressed rat hippocampi and restored depression-like behavioral deficits. Our study presents a novel small-molecule strategy that leverages TTNPB-centered epigenetic remodeling and metabolic reprogramming as dual mechanisms driving neural differentiation.
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