JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
·
2016
·Vol. 21
·期 8
·945-956
·
IF 2.495
Sun Dongdong,Mou Zhipeng,Li Nuan,Zhang Weiwei,Wang Yazhe,Yang Endong,Wang Weiyun
Two new ruthenium (II) polypyridyl complexes [Ru(MeIm) 4 (pip)] 2+ ( 1 ) and [Ru(MeIm) 4 (4-npip)] 2+ ( 2 ) were synthesized under the guidance of computational studies (DFT). Their binding property to human telomeric G-quadruplex studied by UV–Vis absorption spectroscopy, the fluorescent resonance energy transfer (FRET) melting assay and circular dichroism (CD) spectroscopy for validating the theoretical prediction. Both of them were evaluated for their potential anti-proliferative activity against four human tumor cell lines. Complex 2 shows growth inhibition against all the cell lines tested, especially the human lung tumor cell (A549). The RTCA analysis not only validated the inhibition activity but also showed the ability of reducing A549 cells’ migration. DNA-flow cytometric analysis, mitochondrial membrane potential (ΔΨm) and the scavenger measurements of reactive oxygen species (ROS) analysis carried out to investigate the mechanism of cell growth inhibition and apoptosis-inducing effect of complex 2 . The results demonstrated that complex 2 induces tumor cells apoptosis by acting on both mitochondrial homeostasis destruction and death receptor signaling pathways. And those suggested that complex 2 could be a candidate for further evaluation as a chemotherapeutic agent against human tumor.
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高影响因子
CELLULOSE
·
2019
·Vol. 26
·期 13
·8087-8099
·
IF 3.917
Zhang Su-Feng,Liu Li-Na,Tang Rui-Hua,Liu Zhi,He Xiao-Cong,Qu Zhi-Guo,Li Fei
Abstract Lateral flow assays (LFAs) have been extensively used as point-of-care testing platforms because they are inexpensive, portable, simple, and rapid, which particularly improve their availability in resource-poor settings. However, the poor sensitivity of LFAs restricts their further applications. Herein, we proposed a novel and simple method to enhance the detection sensitivity of LFAs by embedding cotton thread-based barriers into paper and further integrating them into strips to decrease the flow rate of sample and extend the reaction time. The number and hydrophilicity of the embedded cotton threads were sequentially optimized. The flow rate of liquid in cotton thread-embedded LFAs was mathematically simulated using a circuit-like model and the simulation results are consistent with the experimental results. With using human immunodeficiency virus nucleic acid as a model target, the cotton thread-embedded LFAs presented a fourfold enhancement in detection sensitivity compared to that of the unmodified LFAs. The strategy of embedding cotton threads into paper possesses great potential for fabricating other paper-based microfluidic devices in the future. Graphic abstract
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-6
·
IF 5.705
Liu Renjie,Wu Hua,Lv Lei,Kang Xiaojiao,Cui Chengbi,Feng Jin,Guo Zhijun
This study describes an aptamer based assay for the mycotoxin ochratoxin A (OTA). The method is based on the use of an OTA-specific aptamer, exonuclease (Exo) III, SYBR Gold as a fluorescent probe, and a complementary strand that specifically combines with the aptamer. In the presence of OTA, the aptamer and OTA hybridize, thereby resulting in the formation of ssDNA, which is not digested by Exo III. Intense fluorescence is observed after addition of SYBR Gold (best measured at excitation/emission wavelengths of 495/540 nm). Fluorescence increases linearly with the log of the OTA concentration in the range from 8 to 1000 ng · mL −1 . The detection limit is 4.7 ng·mL −1 . The assay was applied to the determination of OTA in diluted [2%( v /v)] red wine, and recoveries and RSDs ranged between 93.5% and 113.8%, and between 3.2% and 5.7%, respectively.
In the presence of ochratoxin A (OTA), specific combinations of aptamer and OTA may occur and result in DNA double strands being untied, which avoids being digested by Exo III. Intense fluorescence is observed after SYBR Gold addition.
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高影响因子
ARCHIVES OF VIROLOGY
·
2016
·Vol. 161
·期 8
·2197-2205
·
IF 2.255
Shi Xiaodan,Chen Zijian,Tang Shengjie,Wu Fei,Xiong Sidong,Dong Chunsheng
Autophagy is an intrinsic cellular process that can degrade cytoplasmic components. It has been reported that several pathogens hijack this process to facilitate their replication. Coxsackievirus B3 (CVB3), a member of the family Picornaviridae , induces autophagy upon infection. However, the details of CVB3-induced autophagy remain a subject of debate. This study applied a combination of multiple assays for the measurement of autophagy and demonstrated that CVB3 induces a complete autophagic flux. Experiments with infected HEK293A cells revealed that autophagosomes were induced upon CVB3 infection. Most of these autophagosomes were mCherry positive in mCherry-GFP-LC3 cells. Conversely, mCherry-positive autophagosomes were rescued to green positive when treated with the acidification inhibitors chloroquine (CQ) and bafilomycin A1 (BAF), suggesting that autophagosomes fused with late endosomes or lysosomes. The co-localization of LC3-positive puncta with lysosome-associated membrane protein 1 (LAMP1) or LysoTracker confirmed that the autophagosomes fused primarily with lysosomes. Interestingly, the disruption of autophagosome formation by 3-methyladenine (3-MA) or ATG5 siRNA treatment during viral infection significantly decreased CVB3 replication. However, inhibitors of lysosomal acidification, fusion, or degradation did not affect viral replication. Therefore, autolysosomes may not be critical for viral replication in vitro .
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高影响因子
NEUROTOXICITY RESEARCH
·
2022
·Vol. 40
·期 6
·1664-1672
·
IF 3.978
Zhou Changlong,Wang Jinping,Shao Gaohai,Xia Xiaohui,Wu Lirong,Yu Anyong,Yang Zhao
Background Mild hypothermia has been identified to reduce brain injury following intracerebral hemorrhage (ICH) by protecting neuron cells through several pathways. However, the role of hypothermia in brain function following ICH and the related mechanisms have not been well identified. Ubiquitination-mediated inflammation plays important roles in the pathogenesis of immune diseases. The experiment analyzed anti-inflammatory effects of mild hypothermia following ICH. Methods The model of ICH was induced by injecting autologous blood. Neuregulin receptor degradation protein-1 (Nrdp1) and downstream molecule were analyzed. In addition, brain inflammatory response, brain edema, and neurological functions of ICH mice were also assessed. Results We found that mild hypothermia attenuated proinflammatory factors production after ICH. Mild hypothermia significantly inhibited BBB injury, water content, and neurological damage following ICH in vivo. Moreover, mild hypothermia also increased Nrdp1/MyD88 levels and thus affect neuronal apoptosis and inflammation. Conclusions Taken together, these results suggest that mild hypothermia can attenuate the neuroinflammatory response and neuronal apoptosis after ICH through the regulation of the Nrdp1 levels.
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Journal of Neuroinflammation
·
2021
·Vol. 18
·期 1
·1-13
·
IF 8.322
Huang Xuelin,Deng Jie,Xu Ting,Xin Wenjun,Zhang Yuehong,Ruan Xiangcai
Background We previously reported a correlation between small doses of oxaliplatin penetrating onto the spinal cord and acute pain after chemotherapy. Here, we propose that MT2 within the spinal dorsal horns participates in the development of oxaliplatin-induced neuropathic pain and may be a pharmacological target for the prevention and treatment of chemotherapy-induced peripheral neuropathy (CIPN).Methods The rat model of CIPN was established by 5 consecutive injections of oxaliplatin (0.4 mg/100 g/day). Genetic restoration of neuron-specific metallothionein-2 was implemented 21 days before oxaliplatin treatment, and also, genetic inhibition by metallothionein-2 siRNA was performed. Mechanical allodynia and locomotor activity were assayed. Cell-specific expression of metallothionein-2, the mRNA levels of pro-inflammatory cytokines, nuclear translocation of NF-κB, the protein levels of expression of IκB-α, and interaction between IκB-α and P65 were evaluated in the spinal dorsal horns. Also, in vitro interaction of sequentially deleted IκB-α promoter with metallothionein-2 was used to assess the signal transduction mechanism.Results We found that oxaliplatin induced downregulation of metallothionein-2 in rat spinal cord neurons. By contrast, genetic restoration of metallothionein-2 in the spinal dorsal horn neuron blocked and reversed neuropathic pain in oxaliplatin-treated rats of both sexes, whereas genetic inhibition of metallothionein-2 triggered neuropathic pain in normal rats. Overall locomotor activity was not impaired after the genetic alterations of metallothionein-2. At the molecular level, metallothionein-2 modulated oxaliplatin-induced neuroinflammation, activation of NF-κB, and inactive transcriptional expression of IκB-α promoter, and these processes could be blocked by genetic restoration of metallothionein-2 in the spinal dorsal horn neurons.Conclusion sMetallothionein-2 is a potential target for the prevention and treatment of CIPN. A reduction of NF-κB activation and inflammatory responses by enhancing the transcription of IκB-α promoter is proposed in the mechanism.
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Nature Communications
·
2025
·
IF 15.7
Peng Cao,Qilin Li,Yuyu Li,Honglian Guo,Chunyu Wei,Rengui Xu,Chenke Ouyang,Wei Chen,Lin Wang,Zheng Wang
Probiotic-based biotherapy for colorectal cancer (CRC) faces significant challenges due to poor tumor-targeting and limited bioactivities within the complex tumor microenvironment. This study shows that Lactobacillus reuteri (L. reuteri )’s inherent bioactivities, including proliferation, metabolism, and colorectal colonization, can be enhanced by manganese, prompting the fabrication of a manganese-engineered strain (MnLR). Oral administration of MnLR with its metabolic substrate-glycerol (MnLR/Gly) promotes the enrichment of L. reuteri and its antitumor metabolites within colon tumors. Across multiple preclinical colon tumor models, MnLR/Gly alone achieves a 95.6% inhibition of orthotopic tumor growth, liver metastases reduction by 62.1%, and confers durable protection, with 75% of prophylactically treated mice remaining long-term tumor-free and 62.5% resisting tumor rechallenge. Mechanistically, MnLR/Gly induces both intratumoral and peripheral dendritic cell maturation, M1 macrophage polarization, and effector T-cell responses. In orthotopic CRC rabbit models, GlyMnLR enteric capsules demonstrate significant antitumor efficacy comparable to standard chemotherapy while exhibiting favorable safety. These findings highlight MnLR as an effective standalone probiotic therapy for CRC prevention and treatment. Probiotic therapy for colorectal cancer is limited by the poor tumor targeting and low intratumoral activity. The authors show that manganese-engineered L. reuteri with glycerol (MnLR/Gly) accumulates in colorectal tumors, produces cancer-selective metabolites, activates antitumor immunity, and achieves potent, durable tumor suppression in preclinical models.
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产品关联
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PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 12
·8786-8794
·
IF 2.993
Wang Xiaoqiang,Xu Baomei,Liu Zhenzhen
Supramolecular anchoring of metalloporphyrins in a protein is an attractive approach to the generation of artificial enzymes. Here, we employ the hydrophobic nanocage of single-ring mutant of bacterial GroEL protein for this purpose. We found that multiple monomeric hemin cofactors can be efficiently loaded into the protein nanocage. The as-prepared biohybrid possessed an oxidase-like catalytic activity and followed the typical Michaelis–Menten kinetics and a ping-pong mechanism in the H 2 O 2 -mediated oxidation of model substrates. In comparison with natural peroxidase, the artificial enzyme exhibited higher affinity for the model substrate. A simple and sensitive colorimetric method for the quantitative detection of H 2 O 2 and glucose was also developed based on the artificial enzyme, with the detection limits determined to be 3.0 μM for H 2 O 2 and 5.0 μM for glucose, respectively. The protein nanocage-based artificial enzyme is very flexible and is envisioned to be adapted readily for binding other metal complexes and catalysis of other reactions.
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高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 4
·721-728
·
IF 1.071
Shen Xiaolong,Liu Shaoying,Wang Qingyin,Zhang Hua,Wang Gongying
MgO loaded with KF was prepared by using the impregnation method and was employed as the catalyst for the direct transesterification of diphenyl carbonate(DPC) with isosorbide to synthesize high-molecular-weight poly(isosorbide carbonate)(PIC). The relationship between physical-chemical properties and catalytic performance for KF/MgO in this melt process was investigated by various characterization techniques. The basic site amount and strength were found to be responsible for this transesterification process, and the medium and strong basic sites tended to promote the polycondensation reaction. 20-KF/MgO-500 exhibited the best catalytic performance, giving PIC with M w of 84200 and glass transition temperature( T g ) of 173 °C under optimal conditions. Additionally, 20-KF/MgO-500 was found to catalyze the transerification of DPC with isosorbide and other diols to synthesize the corresponding poly(aliphatic diol-co-isosorbide carbonate)s(PAICs). This excellent activity can be ascribed to the presence of an abundance of basic sites and their specific basic strength on the surface of KF/MgO.
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高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2024
·Vol. 39
·期 6
·1348-1357
·
IF 1.3
Zhang Xiaoli,Li Tingxue,Wang Qinghua,Yang Yan,Zhang Chenyu,Liu Yaning,Wang Yifeng
On the basis of coordinated electrodeposition of carboxylated chitosan (CCS), we presented a green method to prepare Cu NCs and Cu NCs/CCS nanocomposite films. The method shows a range of benefits, such as the convenient and eco-friendly process, mild conditions, and simple post-treatment. The experimental results reveal that a homogeneous deposited film (Cu NCs/CCS nanocomposite film) is generated on the Cu plate (the anode) after electrodeposition, which exhibits an obvious red florescence. The results from TEM observation suggest there are nanoparticles (with the average particle size of 2.3 nm) in the deposited film. Spectral analysis results both demonstrate the existence of Cu NCs in the deposited film. Moreover, the Cu NCs/CCS film modified electrode is directly created through electrodeposition of CCS, which enables promising application in the electrochemical sensing. By means of fluorescence properties of Cu NCs, the Cu NCs/CCS film also owns the potential in fluorescence detection. Therefore, this work builds a novel method for the green synthesis of Cu NCs, meanwhile it offers a convenient and new electrodeposition strategy to prepare polysaccharide-based Cu NCs nanocomposites for uses in functional nanocomposites and bioelectronic devices.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2024
·
IF 4.1
Zhang Xin-Rao,Wang Fu-Shan,Wang Xin,Gao Yan,Zhang Hong-Xing,Liu Zhi-Qin,Feng Jia-Chun
Although Ziegler-Natta (Z-N) polyolefins have been widely used as raw materials to produce pharmaceutical or food packaging, the migration of acid scavengers, an additive usually introduced in Z-N polyolefins, from the packaging to its contents has not been reported. In this work, the migration of the two most used acid scavengers, calcium stearate (CaSt 2 ) and zinc stearate (ZnSt 2 ), from a Z-N polypropylene random copolymer (PPR) into water during autoclaving at 121 °C were comparatively investigated. It was found that, for PPR plates containing 0.1 wt% CaSt 2 or ZnSt 2 (PPR-0.1CaSt 2 , PPR-0.1ZnSt 2 , respectively), the concentration of migrated calcium ion into water increased with autoclaving time, while that of zinc ion was much lower at same treatment durations and did not show a significant increase with treatment time. Interestingly, after removing all plates and acidification treatment, a considerable amount of stearic acid was detected in sterilized water for PPR-0.1ZnSt 2 , but no such significant stearic acid was detected in sterilized water for PPR-0.1CaSt 2 . Based on the structural evolution of the two soaps upon heating, possible mechanisms for the different migration behavior of CaSt 2 and ZnSt 2 from PPR into water during autoclaving treatment were proposed. Our results suggest that the migration issue of acid scavengers is worthy of attention in pharmaceutical packaging materials produced from Z-N polyolefins.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2024
·
IF 1.5
Liu Nana,Xu Yunan,Zhan Chenghua,Li Yahe,Yuan Yijun
Uranium extraction from seawater is urgently required for the sustainable development of nuclear industry. Herein, the application potential of Thalassiosira pseudonana adsorbing U(VI) was examined. A 0.3 g L −1 adsorbent dose at an initial uranium concentration of 8 ppm, pH 5.0, and a contact time of 8 h achieved maximum U(VI) adsorption (23.75 mg g −1 ) at 298 K. The adsorption process was a chemisorption phenomenon, characterized by homogeneous monolayer adsorption. The adsorbents exhibited high hydrophilicity, with –OH and –COOH being the main functional groups for uranium adsorption. T. pseudonana had excellent regeneration performance and high sorption selectivity. The research denoted T. pseudonana is an effective and promising sorbent for extracting uranium from seawater.
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