Cell Chemical Biology
·
2020
·
IF 7.739
Giorgia Croppi,Yueyang Zhou,Rong Yang,Yunfei Bian,Mingtao Zhao,Youtian Hu,Benfang Helen Ruan,Jing Yu,Fang Wu
Summary H 2 S-producing enzymes in bacteria have been shown to be closely engaged in the process of microbial survival and antibiotic resistance. However, no inhibitors have been discovered for these enzymes, e.g., 3-mercaptopyruvate sulfurtransferase (MST). In the present study, we identified several classes of inhibitors for Escherichia coli MST (eMST) through high-throughput screening of ∼26,000 compounds. The thiazolidinedione-type inhibitors were found to selectively bind to Arg178 and Ser239 residues of eMST but hardly affected human MST. Moreover, the pioglitazone of this class concentration dependently accumulates the 3-mercaptopyruvate substrate and suppresses the H 2 S and reactive sulfane sulfur products in bacteria. Importantly, pioglitazone could potentiate the level of reactive oxygen species in cellulo and consequently enhance the antimicrobial effects of gentamicin and macrophages in culture. This study has identified the bioactive inhibitor of eMST, paving the way for the pharmacological targeting of eMST to synergistically control the survival of E. coli .
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高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Min Mu,Yuelong Wang,Shasha Zhao,Xiaoling Li,Rangrang Fan,Lan Mei,Min Wu,Bingwen Zou,Na Zhao,Bo Han,Gang Guo
Chemotherapy works against tumors by inducing cell apoptosis; however, evasion of apoptosis is recognized to result in resistance to anticancer therapy. Ferroptosis is an iron-dependent cell death pathway that differs from apoptosis in morphological, biochemical, and genetic levels. Combined ferroptosis and apoptosis may shed light on strategies for cancer treatment. Therefore, we have designed a nanoparticle (NP) that can simultaneously cause tumor cell apoptosis and ferroptosis. This NP is composed of epigallocatechin gallate (EGCG) and Fe3+ through a simple and green process and can be used to deliver doxorubicin hydrochloride (DOX) and iron ions to the tumor site at the same time. DOX/Fe3+/EGCG (DF) NPs display a great resolubility and long-term storage stability, and efficient DOX and Fe3+ release is realized after cellular internalization under the high level of glutathione and acidic nature in tumor. EGCG is likely to chemically reduce the released Fe3+ to Fe2+. The generated Fe3+/Fe2+ converts intracellular H2O2 to hydroxyl radicals (•OH) via the Fenton reaction. In addition, the generated •OH subsequently induces lethal ferroptosis to improve DOX-induced apoptosis. In vitro and in vivo investigations indicate that a great therapeutic effect was achieved, suggesting that the formation of the DF NP delivery system is a promising strategy to fight against tumors by an apoptosis and ferroptosis combination modality.
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MOLECULES
·
2023
·Vol. 28
·期 17
·6321
·
IF 4.6
Jing Yang,Liyang Wu,Tao Wang,Yiqing Zhao,Xiaoqian Zheng,Yongping Liu
To improve the utilization value of raspberry leaves, the extraction and purification conditions of phenolic compounds from raspberry leaves were optimized, and the contents of phenolic compounds and the biological activities of extracts were studied. After steam explosion pretreatment at 115 °C for 15 min, raspberry leaf extract with a total phenolic content (TPC) of 136.30~140.51 mg GAE/g was obtained via homogenization and ultrasound-assisted extraction. In addition, the adsorption relationship between raspberry leaf polyphenols and middle polar XDA-6 macroporous resin was best described by the Langmuir model, and tended to be monolayer adsorption. Its adsorption kinetics best resembled the pseudo second-order kinetic model, and it was speculated that this was influenced by multiple factors. According to the optimal integrated extraction–purification process, the TPC of the extracts increased to 738.98 mg GAE/g after one application of purification and 905.27 mg GAE/g after two applications of purification. Moreover, the latter case showed the highest antioxidant activity and α-glucosidase inhibition activity, and the content of the most typical compound, quercetin-3-glucuronide, reached 199.69 mg/g. SE has a double-edged effect, and is more conducive to the release of active substances as a pre-treatment method. This study provides a theoretical basis for the efficient use of raspberry leaves, further improving their medicinal and economic value.Keywords:steam explosion;XDA-6;Rubus ideaus;quercetin-3-glucuronide
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高影响因子
Soft Science
·
2026
·Vol. 6
·期 4
·N
·
IF 15.9
Xinlin Du\u003Csup\u003E1\u003C\u002Fsup\u003E,Chenyi Shi\u003Csup\u003E2\u003C\u002Fsup\u003E,Qianwang Ye\u003Csup\u003E1\u003C\u002Fsup\u003E,Liwei Wang\u003Csup\u003E1\u003C\u002Fsup\u003E,Ruiyang Xiao\u003Csup\u003E1\u003C\u002Fsup\u003E,Weijin Zhang\u003Csup\u003E1\u003C\u002Fsup\u003E,Le Wang\u003Csup\u003E1,\u003C\u002Fsup\u003E,Ruiqian Guo\u003Csup\u003E1,\u003C\u002Fsup\u003E,Hanqing Dai\u003Csup\u003E1,\u003C\u002Fsup\u003E
Endogenous electric field (EF) plays a crucial role in the wound healing process, yet conventional electrical stimulation strategies remain constrained by their dependence on bulky external power supplies. Herein, we report a bilayer electrogenerative smart dressing (B-ESD) that generates a self-sustained EF through a robust streaming potential induced by unidirectional pumping of wound exudate. This biofluid-driven mechanism successfully amplified the attenuated wound potential from 343.8 to 921.0 mV without requiring any external power source. Comprehensive in vitro and in vivo evaluations demonstrated that this self-sustained EF mitigated acute inflammation, accelerated fibroblast migration, and promoted highly organized collagen deposition. Immunohistochemical analysis further revealed that the B-ESD regulated the expression of Type I collagen, CD31, and transforming growth factor-beta 1 at the wound site, improving the quality of wound repair. Consequently, the B-ESD achieves a 98.72% wound closure rate within 14 days, with reduced scar formation and the regrowth of mature skin appendages. Moreover, the B-ESD serves as a high-fidelity sensor to monitor physiological motions, offering a promising integrated platform that combines bioelectric wound therapy with real-time biomechanical monitoring for advanced wound management in the future.
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Nanoscale Advances
·
2022
·Vol. 4
·期 19
·4169-4174
·
IF 5.598
Yi Gao,Shaofeng Liang,Shuangliang Zhao,Wei Gao,Zequan Li,Muqun Wang,Hong Li,Xiangning He,Riyao Cong,Hailin Diao,Chuwang Su,Xiaoying Xie
The emergence of cooling technology has brought about huge social benefits to society, but it is also accompanied by the serious problem of energy consumption. In countries close to the equator, intense solar radiation is accompanied by unbearable high temperatures and strong ultraviolet radiation. Therefore, we prepared a simple hydrogel with good evaporative cooling, which can work continuously and has good UV absorption, to solve the indoor cooling and UV radiation problems. Polyacrylamide (PAM) in the hydrogel provides a mechanically strong backbone, and polyethylene glycol (PEG) slows water loss and provides the hydrogel with the ability to reflect infrared light. Lithium bromide (LiBr) is a highly efficient water vapor absorbent, which can provide the hydrogel with water regeneration capability. Carbon dots (CDs) can provide excellent UV absorption for hydrogels, and CDs (4.28 kJ kg−1 K−1) have a higher specific heat capacity than water (4.20 kJ kg−1 K−1), which can store more heat for a better indoor cooling effect. The composite hydrogel has a good prospect of application in the windows of residential and high-rise buildings.
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高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2017
·
IF 1.392
Liqiang Liu,Steven Suryoprabowo,Qiankun Zheng,Shanshan Song,Hua Kuang
An immunochromatographic strip was developed for detection of acetamiprid (AC) and cross-reactivity with thiacloprid (TC) in cucumber and apple samples. This detection system was developed based on a sensitive and specific monoclonal antibody produced in our laboratory. Under optimized conditions, the cut-off limits of the test strip for AC were found to be 1 and 0.25 ng/mL for TC in phosphate-buffered saline. Meanwhile, with simple preparation for detection cucumber and apple samples, the cut-off limits for AC were found to be 5 and 2.5 ng/mL for TC in cucumber samples. The immunochromatographic assay also revealed AC cut-off values of 30 and 15 ng/mL in apple samples. Each test can be evaluated within 5 min. The data indicate that the method is sensitive, rapid, specific, and has the advantages of simplicity; therefore, this immunochromatographic assay is a useful screening method for detection of AC and TC residues in cucumber and apple samples.
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高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-16
·
IF 14.919
Wang Yongchao,Gong Ningqiang,Ma Chi,Zhang Yuxuan,Tan Hong,Qing Guangchao,Zhang Jimei,Wang Yufei,Wang Jinjin,Chen Shizhu,Li Xianlei,Ni Qiankun,Yuan Yuan,Gan Yaling,Chen Junge,Li Fangzhou,Zhang Jinchao,Ou Caiwen,Zhao Yongxiang,Liu Xiaoxuan,Liang Xing-Jie
Immunological adjuvants are essential for successful cancer vaccination. However, traditional adjuvants have some limitations, such as lack of controllability and induction of systemic toxicity, which restrict their broad application. Here, we present a light-activable immunological adjuvant (LIA), which is composed of a hypoxia-responsive amphiphilic dendrimer nanoparticle loaded with chlorin e6. Under irradiation with near-infrared light, the LIA not only induces tumour cell lysis and tumour antigen release, but also promotes the structural transformation of 2-nitroimidazole containing dendrimer to 2-aminoimidazole containing dendrimer which can activate dendritic cells via the Toll-like receptor 7-mediated signaling pathway. The LIA efficiently inhibits both primary and abscopal tumour growth and induces strong antigen-specific immune memory effect to prevent tumour metastasis and recurrence in vivo. Furthermore, LIA localizes the immunological adjuvant effect at the tumour site. We demonstrate this light-activable immunological adjuvant offers a safe and potent platform for in situ cancer vaccination.
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高影响因子
JOURNAL OF FOOD QUALITY
·
2022
·
IF 3.2
Xue Zhang,Shuangbo Liu,Bijun Xie,Zhida Sun
This research aimed to investigate an approach to processing more bioavailable chickpea milk by combining enzymolysis and probiotic bacterial fermentation. The regression model of three factors was established using Box–Behnken design (BBD), and the optimum technology of enzymolysis of isoflavone in specimens was determined. Moreover, the variations in isoflavone concentrations in chickpea milk processed with different enzymolysis conditions were explored during fermentation. The isoflavone content was the highest (246.18 mg/kg) when the doses of papain, α-amylase, and β-glucosidase were 75.0 U/g protein, 69.0 U/g starch, and 11.0 U/g chickpea flour. In addition, the contents of isoflavone glucosides decreased and aglycones increased with the prolongation of fermentation. Compared with group C0 (unhydrolyzed specimens), the isoflavone aglycone contents in groups treated with enzymolysis increased to varying degree. Particularly, the isoflavone aglycone contents in group C6 (hydrolyzed with three compound enzymes) were the highest after 24 h fermentation, reaching 56.93 ± 1.61 mg/kg (genistein), 92.37 ± 3.21 mg/kg (formononetin), and 246.18 ± 2.98 mg/kg (biochanin A). The data above indicated that compound enzymolysis coupled probiotic bacterial fermentation could promote the biotransformation of chickpea isoflavone glucosides into aglycones, which might be used as an effective approach to enhance the bioactivity and nutraceutical properties of chickpea milk.
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Nanoscale
·
2017
·Vol. 9
·期 29
·10367-10374
·
IF 7.367
Shanyue Guan,Yangziwan Weng,Mengnan Li,Ruizheng Liang,Chenghua Sun,Xiaozhong Qu,Shuyun Zhou
A supramolecular nanovehicle, denoted as ICG-DOX/Gd-LDH, was synthesized by the co-intercalation of indocyanine green (ICG) and doxorubicin hydrochloride (DOX) into a gallery of Gd3+-doped-layered double hydroxide (Gd-LDH) such that to achieve a chemo-photothermal synergistic therapeutic agent. The unique structure of Gd-LDH can not only stabilize the photothermal agent ICG to enhance the photothermal efficiency, but also hamper the recombination between electron and holes, leading to the generation of more reactive oxygen species (ROS) under irradiation in the NIR range. Together with the loading capacity of DOX, ICG-DOX/Gd-LDH exhibited excellent combinatorial effects on tumor growth inhibition in both in vitro studies on HeLa cell line and in vivo tests over tumor-bearing mouse models. Moreover, it showed ideal ability for long-term tracing of the carrier distribution via either MRI or fluorescence imaging. Thus, this study indicates that Gd-LDH is a promising platform for the construction of multifunctional formulations, especially theranostic nano-systems for cancer treatment.
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高影响因子
Journal of Science-Advanced Materials and Devices
·
2022
·
IF 7.382
Mengru Cai,Boran Ni,Xueling Hu,Kaixin Wang,Dongge Yin,Gongsen Chen,Tingting Fu,Rongyue Zhu,Xiaoxv Dong,Changhai Qu,Xingbin Yin
Metal–organic frameworks (MOFs) with tunable structures, high porosity, and abundant active sites are considered to be promising drug delivery systems (DDSs). In this study, novel MOFs-based nanoparticles ( [email protected] ) were successfully prepared using Zn-based IRMOF-16 as a carrier and curcumin (CUR) as the model drug. This nanocargo delivery platform has dual capabilities, enabling simultaneous drug delivery and fluorescence imaging. In vitro release experiments showed that nanoparticles were released more quickly under mildly acidic conditions, which proved their potential for tumor microenvironment-responsive drug release. Through in vitro cell experiments, it was found that the nano-drug platform had high antitumor cytotoxicity, which may be related to the mechanism of increasing intracellular reactive oxygen species (ROS), reducing intracellular mitochondrial membrane potential (MMP), and inducing apoptosis. In addition, confocal laser microscopy showed that [email protected] could localize to the nucleus to exert an antitumor effect. Meanwhile, [email protected] exhibited superior antitumor activity compared with pure CUR in vitro experiments. The biocompatibility test of IRMOF-16 showed reasonable biosafety, and no evidence of obvious toxicity was observed in vitro. [email protected] has unique advantages with regard to pH response, biocompatibility, and antitumor efficacy, indicating that the nano-drug platform is a promising drug delivery carrier with an antitumor effect.
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Journal of Energy Chemistry
·
2022
·
IF 13.599
Liping Duan,Jianzhi Xu,Yifan Xu,Ruiqi Tian,Yingying Sun,Chuannan Zhu,Xiangyin Mo,Xiaosi Zhou
Potassium ion batteries (PIBs) are emerging as potential next-generation energy storage systems on account of their low cost and high theoretical energy density. Nevertheless, they also face challenges of low specific capacity and suboptimal cycling stability. Herein, we synthesize a cocoon-like P3-type K 0.5 Mn 0.7 Ni 0.3 O 2 (KMNO) cathode material by a self-template method. The KMNO cocoons possess a hierarchical layered architecture composed of nanoparticle stacking, which can accelerate the transport kinetics of potassium ions, mitigate the stress caused by K + intercalation and deintercalation, and improve structural stability. In addition, Ni can not only alleviate the Jahn-Teller distortion and suppress the phase transition to stabilize the structure, but also act as an electrochemically active element, providing the capacity of two electrons from Ni 2+ to Ni 4+ . Combining the advantages of structure and nickel substitution, the P3-type KMNO cocoons are used for electrochemical performance testing of PIB cathodes, delivering an excellent rate capability of 57.1 mA h g −1 at 500 mA g −1 and a remarkable cycling stability of 77.0% over 300 cycles at 100 mA g −1 . Impressively, the KMNO cocoons//pitch-derived soft carbon assembled full battery exhibits superior electrochemical performance with a reversible capacity of 79.7 mA h g −1 at 50 mA g −1 . Moreover, ex-situ XRD also further reveals a solid solution phase reaction with a volume change of only 1.46%. This work furnishes a suitable approach to fabricating high-performance layered oxide cathodes for PIBs with outstanding cycling stability and rate capability.
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Catalysis Science & Technology
·
2022
·Vol. 12
·期 5
·1628-1636
·
IF 6.177
Peng Gao,Peng Wang,Xiaolei Liu,Zihao Cui,Yaqiang Wu,Xiaohan Zhang,Qianqian Zhang,Zeyan Wang,Zhaoke Zheng,Hefeng Cheng,Yuanyuan Liu,Ying Dai,Baibiao Huang
Dry reforming of CH4 by photothermal catalysis is considered to be a promising approach to produce syngas. It can not only store useful chemical energy, but also consume CO2 and CH4 to mitigate the greenhouse effect. Herein, we anchor Ag/AgBr on CsPbBr3 through an in situ ion exchange route to construct a composite material, which exhibits an excellent photothermal synergistic effect in the dry reforming of CH4. The CO production rate of the 7 wt% Ag/AgBr/CsPbBr3 composite through photothermal catalysis is 456.15 μmol g−1 h−1, which is approximately 79 times and 195 times higher than that through pure thermal catalysis and pure photocatalysis, respectively. Moreover, the CO production rate of 7 wt% Ag/AgBr/CsPbBr3 is 7.25 times higher than that of pristine CsPbBr3. The mutual promotion of the thermochemical and photochemical processes significantly improves the energy utilization efficiency and reaction kinetics, leading to high photothermal catalytic efficiency. This work indicates that the inorganic halide perovskite shows excellent photothermal catalytic activity, and provides an alternative way to extend the application range of perovskite materials.
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