CHROMATOGRAPHIA
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2022
·Vol. 85
·期 3
·221-231
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IF 2.213
Sun Zhiping,He Lingli,Yang Qingqing,Zhang Haizhi,Xu Weiren,Qin Xinguang,Liu Gang,Hu Zhongze,Zhang Luyong,Liu Changxiao
The influence of genetic polymorphism of metabolic enzymes on drug–drug interactions (DDI) should be thoroughly investigated owing to its remarkable effect on therapeutic treatments that are futile. Here, different from earlier articles focused on various CYP subfamilies and their isoforms, the effect of genetic polymorphs of a particular isoform, CYP2C9, was comprehensively studied. Using diclofenac as probe substrate, the influence of three CYP2C9 variants (CYP2C9*1, *2 and *3) on DDI was conducted from the measurement of inhibitory ability of six drugs towards three variants. A modified LC–MS/MS method according to former report was constructed for the determination of 4ʹ-hydroxydiclofenac in CYP2C9 enzyme incubation system and validated for accuracy, precision, linearity range within the acceptance criteria for regulatory guidelines. After selecting appropriate incubation time and enzyme concentration, the kinetic parameters of three CYP2C9 variants towards diclofenac were examined and half inhibitory concentrations (IC 50 ) of six drugs towards three CYP2C9 variants were determined. IC 50 of paroxetine and losartan towards CYP2C9*2 were almost threefold higher and 2.5-fold lower than that towards CYP2C9*1, respectively. IC 50 of glibenclamide towards CYP2C9*3 was twofold more than that towards CYP2C9*1. Our results could offer effective guidelines for co-administration of these six drugs with various CYP2C9 substrates in individuals carrying variant CYP2C9 alleles, also broaden the application area of LC–MS/MS.
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CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Dan Xu,Jianfeng Li,Boyang Li,Huacheng Zhao,Hanghang Zhu,Jinfang Kou,Fengwei Zhang,Zhengping Dong,Jiantai Ma
The sustainable catalytic transformation of alcohols to high value-added fine chemicals is a significant and challenging research topic. Herein, a set of nitrogen-doped carbon encapsulated Co-based catalysts (Co-Co 3 O 4 @NC-T) were prepared by using low-cost dicyandiamide, glyoxal and cobalt nitrate as precursors. The obtained catalysts were utilized for the selective oxidation of alcohols to high value-added esters and carboxylic acids with air as the oxygen source and displayed wide applicability for the oxidation of both aromatic and aliphatic alcohols. Based on the controlled experiments, the protective effect of N-doped carbon structure and the synergistic effect between Co core and Co 3 O 4 species guaranteed the high reaction conversion and selectivity. Benefitting from heterogeneity and magnetism of the catalyst, it can be easily recycled and reused for long-term stability. Reasonable mechanisms of selective oxidation reaction were proposed through EPR analysis and controlled experiments. The present work provides a facile strategy for potential large-scale preparation of heterogeneous catalyst for sustainable and green catalytic transformations.
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FUEL
·
2022
·
IF 8.035
Xing Gao,Yingying Ding,Lilin Peng,Dan Yang,Xiaoyue Wan,Chunmei Zhou,Wen Liu,Yihu Dai,Yanhui Yang
Aqueous phase hydrogenative rearrangement of furfural (FAL) to cyclopentanone (CPO) via furfuryl alcohol (FOL) has been studied over Pd catalysts supported on H–ZSM–5 zeolites. Pd states, zeolite properties and reaction condition parameters were optimized, affording 98% selectivity and 120 h −1 specific reaction rate over 2% Pd/H–ZSM–5(25) catalyst at 160 °C and 3 MPa H 2 . For hydrogenation of FAL–to–FOL, the activity was related to the Si/Al ratio and acid property of zeolite supports. For hydrogenative rearrangement of FOL–to–CPO, pure H–ZSM–5 zeolite could catalyze single–step conversion with relatively low reaction rate, whereas the presence of Pd sites could achieve multi–step transformation with remarkably increased rate, highlighting Pd–zeolite acid sites synergy. Proton and furanylmethoxy–relevant intermediates were captured with spin trapping electron paramagnetic resonance experiments, and 4–hydroxy–2–cyclopentenone and 2–cyclopentenone were also identified as key intermediates in tandem reaction pathway to produce CPO.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 1
·1-9
·
IF 6.408
Ma Junlin,Du Yuhang,Jiang Yu,Shen Liuxue,Ma Hongting,Lv Fengjuan,Cui Zewei,Pan Yuzhen,Shi Lei,Zhu Nan
Wearable film-based smart biosensors have been developed for real-time biomolecules detection. Particularly, interfacial co-assembly of reduced graphene oxide-prussian blue (PB-RGO) film through electrostatic interaction has been systematically studied by controllable pH values, achieving optimal PB-RGO nanofilms at oil/water (O/W) phase interface driven by minimization of interfacial free energy for wearable biosensors. As a result, as-prepared wearable biosensors of PB-RGO film could be easily woven into fabrics, exhibiting excellent glucose sensing performance in amperometric detection with a sensitivity of 27.78 µA mM −1 cm −2 and a detection limit of 7.94 μM, as well as impressive mechanical robustness of continuously undergoing thousands of bending or twist. Moreover, integrated wearable smartsensing system could realize remotely real-time detection of biomarkers in actual samples of beverages or human sweat via cellphones. Prospectively, interfacial co-assembly engineering driven by pH-induced electrostatic interaction would provide a simple and efficient approach for acquiring functional graphene composites films, and further fabricate wearable smartsensing devices in health monitoring fields. Graphical abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Dandan Cui,Jin Yang,Bosi Lu,Lansheng Deng,Hong Shen
Mantis shrimp waste (Oratosquilla oratoria) is a good source of chitin. The applicability of microwave-assisted organic acids and proteases for extracting chitin from mantis shrimp shell waste was evaluated, and the extracted-chitin was characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). Moreover, the effects of nanochitin on the growth of pak choi ( Brassica campestris L.ssp.) were also investigated. The results indicated that alkaline protease (4000 U/g, microwave heating at 60 °C, 10 min) and malic acid (5%, 320 W, 5 min) exhibited excellent potential for deproteinizing and demineralizing shells. The deproteinization and demineralization yields were 92.78% and 94.11%, respectively, and the chitin yield was 15.6%. The extracted-chitin had a highly porous structure and exhibited excellent crystallinity and thermostability compared with chitin prepared by traditional chemical methods. Furthermore, 0.003% nanochitin significantly enhanced photosynthesis, which improved the pak choi fresh weight by 22.94%, and improved the accumulation of isothiocyanates in its leaves. This study provides an alternative approach for the high-value utilization of mantis shrimp waste, and reveals the potential of chitin for application in agricultural production.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Yi Han,Mingjie Huang,Wei Xiang,Chen Wang,Yongjie Li,Xiaohui Wu,Juan Mao,Tao Zhou,Hong Li,Deming Wu
Fe-based metal–organic frameworks (MOFs) are popular in activating oxidants e.g·H 2 O 2 and persulfate (PS) for degrading recalcitrant organic substances, generally regarded with a simple interfacial efficient iron cycle. This study has revealed that a novel two-steps single-electron transfer mechanism would occur initially to activate the rapid degradation 4-chlorophenol (4-CP) in the MIL-88B(Fe)/H 2 O 2 system (88B/H 2 O 2 system). The degradation of 4-CP was acidic favorable (pH 0 ≤ 6.0) and could be divided into two reaction stages, i.e., initial lag phase (0–10 min) and following rapid decay phase (10–50 min). In the first stage, H 2 O 2 was slowly activated on the surface of 88B to form superoxide radical (O 2 •– ), which would lead to the accumulation of the key intermediate hydroquinone (HQ) through reducing 4-CP. Afterwards, HQ could reductively dissolve iron ions which would lead to the subsequent efficient homogeneous Fenton reaction for the rapid oxidation of 4-CP by hydroxyl radical (•OH) in the following rapid decay phase. It was interesting to note that specific aromatic compounds, which would form reductive benzenediols intermediates by O 2 •– , could be efficiently degraded in the 88B/H 2 O 2 system. The result of this study is expected to provide a new insight for understanding the catalytic oxidation process based on MOFs, and its application if possible.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2022
·
IF 24.319
Dongli Guo,Yuan Yao,Shijie You,Limin Jin,Ping Lu,Yanbiao Liu
Herein, we developed and evaluated an electrochemical periodate (PI) activation system for the ultrafast degradation of aqueous micropollutants (τ < 3 s). Filters constructed from carbon nanotubes (CNT) coated with Fe 2 O 3 nanoparticles on the outer (Fe 2 O 3 -out-CNT) and inner surfaces (Fe 2 O 3 -in-CNT) were prepared to regulate the generation of reactive oxygen species (ROS) during PI activation. The activation function of the electroactive nanohybrid filters lay in their ability to facilitate the redox cycling of Fe(III)/Fe(II) assisted by an electric field. The results showed that a non-radical ( i.e ., 1 O 2 ) pathway dominated the degradation process in the electro/Fe 2 O 3 -in-CNT/PI system, while a contrastive radical pathway ( i.e ., HO • and IO 3 •) was identified in the electro/Fe 2 O 3 -out-CNT/PI system. The electro/Fe 2 O 3 -in-CNT/PI system exhibited enhanced catalytic activity towards the micropollutants degradation relative to its electro/Fe 2 O 3 -out-CNT/PI counterpart. Density functional theory calculations suggested that PI could be directly decomposed under the nanoconfined environment, rather than forming a stable adsorption complex in the unconfined system.
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 14
·6636-6645
·
IF 3.925
Huiting He,Min Zheng,Qiang Liu,Jian Liu,Juan Zhao,Yuting Zhuang,Xianxiang Liu,Qiong Xu,Steven R. Kirk,Dulin Yin
Through the amidation reaction with 2-methylimidazole, organic basic groups were introduced into the carboxyl sites of activated carbon to obtain imidazolyl activated carbon. H3+xPW12−xVxO40 (x = 0, 1, 2, or 3) was chemically bonded to the surface, which was used to catalyze the oxidation of perillyl alcohol by hydrogen peroxide. After reaction at 60 °C for 5 h, AC-COIMIH+[H4PW10V2]− could catalyze almost complete oxidation of the substrate, where the conversion reached 97.4%, the selectivity for the 1,2-epoxide was 80.0%, and the stereo configuration of the epoxide product was RR/SS = 5 : 3. In addition, the catalyst could be easily recovered via centrifugation and reused five times without any significant deactivation. By calculating the Fukui function of perillyl alcohol, we consider a proper explanation for the main product from the oxidation of the allyl alcohol. A cooperative epoxidation mechanism with electrostatic adsorption of a double bond, and hydrogen-bonded adsorption of hydroxyl was proposed.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2022
·
IF 5.062
Guan-Lan Huang,Ling Yang,Bo-Ying Ren,Xin-Yue Lv,Ling-Yi Song,Xia-Lin Dai,Jia-Mei Chen
Vemurafenib (VEM) is a first-line drug for the treatment of metastatic melanoma with BRAF V600 mutation, which was developed in the form of amorphous solid dispersion to overcome the defects of poor aqueous solubility and oral absorption. However, the oral dose of VEM is still very high; besides, the poor stability of amorphous form may increase the risks in drug effectiveness and safety. Herein, we attempted to provide a solution to these issues based on cocrystallization strategy. Three cocrystalline forms involving VEM and D / L / dl -camphorsulfonic acid with stoichiometric ratio of 1:1 were synthesized and characterized by powder X-ray diffraction analysis, 1 H nuclear magnetic resonance, thermal analyses and Fourier transform infrared spectroscopy . The in vitro evaluations show that all of the cocrystalline forms demonstrate improved hygroscopicity and stability compared to amorphous VEM; especially, the cocrystalline form with dl -camphorsulfonic acid exhibits much better tabletability and apparent solubility than all of other forms. Pharmacokinetic tests reveal that all the cocrystallized products show significantly enhanced C max and AUC 0-t values compared to the amorphous form. Therefore, the newly discovered cocrystalline forms of VEM are expected to overcome the issues of poor solubility and oral absorption and provide great potential for the development of improved preparations of the drug.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 6
·3385-3399
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IF 3.612
Yu Zhang,Kexin Li,Maobin Chen,Shangling Fang,Da Zhen,Jinghua Cao,Zhengqi Wu,Ke Zhang
Summary In this study, a novel chrysanthemum polysaccharide (CP) was obtained and investigated from Chrysanthemum morifolium tea in Hubei province, China. Molecular characterisation and emulsifying properties of the CP were elucidated. The effects of different factors, including storage time, metal ions, pH value and heat treatment, were studied on the stability of emulsion. Results showed that the mean particle diameter (MPD) ( d 32 ) varied from 5.54 to 0.49 μm with the polysaccharide concentration ranging from 0.5wt% to 3.0wt%. The MPD ( d 32 ) of emulsions stabilised by chrysanthemum tea polysaccharide (ECP) was smaller than that of GA at 2wt%, and the MPD ( d 32 ) of ECP remained stable with no stratification occurred at 25 °C for 10 days. ECP also showed good stability under acidic conditions (3.0–7.0). With increase of Na + and Ca 2+ concentration, MPD( d 32 ) and zeta potential values raised significantly, from 2.24 to 7.24 μm and from −40.52 to −35.35 mV respectively. After high-temperature heat treatment, MPD ( d 32 ) and zeta potential showed no significant change, indicating ECP presented good storage stability. In conclusion, CP helped stabilised emulsions that could be used as a natural emulsifier for future application.
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ANALYST
·
2022
·Vol. 147
·期 8
·1598-1610
·
IF 5.227
Huizhen Deng,Jie Zhao,Shifan Zhao,Shuai Jiang,Guofeng Cui
Developing an accurate, sensitive, and efficient portable device for simultaneous detection of disease-related biomolecules is an attractive challenge for point-of-care testing (POCT). In this work, we introduced a novel graphene-based electrochemical flow analysis device (GEFAD), which combined a 3D-printed flow cell with a three-electrode system based on a gold nanoparticle-modified graphene (AuNPs@Gr) working electrode to simultaneously detect depression-related biomolecules: dopamine (DA), 5-hydroxytrypserotonin (5-HT), and melatonin (MEL). Electrochemical studies showed that the GEFAD had high selectivity and sensitivity in the determination of DA, 5-HT, and MEL with their significant separation peaks. The linear ranges of DA, 5-HT, and MEL were respectively found to be 0.1–70 μM, 0.1–100 μM, and 0.1–150 μM, with the corresponding detection limits of 0.054 μM, 0.071 μM, and 0.087 μM (S/N = 3). In addition, the GEFAD presented a good anti-interference ability and satisfactory reproducibility, and can be used in real calf serum sample analysis. The introduced in-flow electrochemical detection device showed excellent repeatability, less sample consumption, and simple operation, which increases the possibility to implement POCT. This study also proposed a promising application prospect for the development of portable biomolecules detection devices.
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ACS Biomaterials Science & Engineering
·
2022
·
IF 5.395
Wei Ma,Huanli Zhang,Shuying Li,Zhiqiang Wang,Xiaodan Wu,Rui Yan,Fang Geng,Weijie Mu,Yingxue Jin
Chemodynamic therapy (CDT) based intracellular chemical reactions to produce highly cytotoxic reactive oxygen species has received wide attention. However, low efficiency of single CDT in weakly acidic pH and glutathione (GSH) overexpressed tumor cells has limited its clinical application. For this study were prepared two-dimensional metal–organic framework (MOF) to improve CDT efficiency based on the combined action of bimetallic CDT, consumption of overexpressed glutathione (GSH) in cells, folic acid (FA) induced tumor targeting and triphenylphosphine (TPP) induced mitochondrial targeting. With the use of Cu(II) as the central ion and tetrakis(4-carboxyphenyl)porphyrin (TCPP) as the ligand, two-dimensional Cu-MOF nanosheets were prepared, which were surface modified by manganese dioxide based on the in situ redox reaction between poly(allylamine hydrochloride) (PAH) and KMnO4 to obtain Cu-MOF@MnO2. Then FA and TPP were coupled with the nanosheets to form the title nanoplatform. Comprehensive physiochemical research has suggested that Cu(II) and MnO2 constituents in the nanoplatform could consume intracellular GSH and hydrogen peroxide to generate hydroxyl radicals through a Fenton-like reaction; meanwhile Cu(II) could undergo a Russell reaction to produce cytotoxic singlet oxygen. Detailed in vitro and in vivo biological experiments have revealed a good biosafety profile and a high tumor suppression effect. Therefore, the present research has realized multiple and efficient CDT effects with the aid of the sequential targeting of FA/TPP, also providing a strategy for the development of CDT drugs based on polymetallic organic frameworks.
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