BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-19
·
IF 4.8
Zhang Xiaoxiao,Zhu Lili,Xu Zihan,Wang Zhimin,Dai Liping
Background DNA methylation plays a key role in regulating plant growth and development, secondary metabolism, and environmental adaptability and is affected by DNA methyltransferase (C5-MTase) and demethylase (dMTase). However, the response of C5-MTase and dMTase genes to abiotic stress in Isodon rubescens remains largely unknown. Results In this study, eight IrC5-MTase genes and five IrdMTase genes were identified within the genome of I. rubescens . The IrC5-MTases were phylogenetically divided into four subfamilies (CMT, DRM, MET and DNMT2), and the IrdMTase were divided into two subgroups (ROS and DML) on the basis sequence similarity, conserved motif and domain architecture. The IrC5-MTase and IrdMTase genes were unevenly distributed on 5 and 4 chromosomes, respectively. Cis‑acting element analysis revealed that the IrC5-MTase and IrdMTase genes were enriched with multiple light-, phytohormone- and stress-responsive elements. Importantly, the level of DNA methylation in I. rubescens was influenced by different 5-azacytidine (5-azaC) concentrations or light intensities but unaffected by different NaCl concentrations. Accordingly, the expression of IrC5-MTase and IrdMTase genes were significantly upregulated or downregulated in response to different concentrations of 5-azaC or amounts of light stress, however, no conspicuous differences were observed under different degrees of NaCl stress. Expression correlation analysis revealed that the expression of IrCMT2a , IrMET1 , and IrDML4 was most closely related to the DNA methylation level in I. rubescens . Conclusions Our results reveal the characteristics and differential expression of the IrC5-MTase and IrdMTase genes in response to 5-azaC, light or NaCl stresses and will facilitate future epigenetic studies in I. rubescens .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 11
·5249
·
IF 4.9
Lijun Ding,Xiao Luo,Weijia Wen
Obesity and associated metabolic disorders pose significant health challenges. Fucoxanthin, a lipophilic compound, has shown promising anti-obesity potential, but its poor solubility and bioavailability limit therapeutic efficacy. The successful formulation of solid lipid nanoparticles (SLNs) amplified fucoxanthin’s efficacy in mitigating obesity and the associated metabolic dysregulation. High-fat diet (HFD)-induced obese mice were treated with free fucoxanthin, lyophilized SLNs (L-SLN), and dispersed SLNs (D-SLN) loaded with fucoxanthin. The intervention with D-SLN demonstrated the most significant reduction in body weight gain (29.94%) and fat mass gain (61.80%) compared to the HFD group (p< 0.05), alongside notable improvements in metabolic indicators including fasting blood glucose, liver enzymes, lipid profile, and inflammatory markers such as leptin and monocyte chemoattractant protein 1 (MCP-1) levels. Histopathological evaluation corroborated these findings, showing highly reduced hepatic lipid droplet accumulation and improved adipocyte and testicular morphology. This advancement paved the way for translating fucoxanthin into a clinically effective anti-obesity agent.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xu Zhang,Nanxin Zhang,Mingjin Li,Wencong Zhang,Ning Ji,Jiqing Lei,Haiyan Sun,Bangdi Liu,Cunkun Chen,Pufan Zheng,Rui Wang
Diseases caused by tuber fungi severely disrupt the storage life and quality of post-harvest Gastrodia elata ( G. elata ). Herein, it was found that the decay of G. elata tubers is primarily attributed to Fusarium solani and Penicillium crustosum . To inhibit postharvest rotting in G. elata tubers, microwave-treatment (MT) was employed as a disinfection method. However, they could not be completely inhibited by a single MT in vivo test, which were at various power levels (300, 500, 700 W) and durations (20, 30, 40 s). Hence, MT (700w, 30s) combined with modified atmosphere packaging (MAP) was employed, which were polyethylene films of varying thickness (20, 30, 40 μm), and its efficiency was validated. Results indicate that the combined treatment improved the quality of infected G. elata tubers. In addition, it decreased 79.81% in decay volume of G. elata tubers compared with the control group. Further results indicate that the reactive oxygen species and energy metabolism pathways of G. elata tubers were activated by the combined treatment, activating the enzyme activity and relative expression of superoxide dismutase, catalase and peroxidase and maintaining the levels of ATP, ADP and energy charge. Taken together, these results indicate that MT combined with MAP is a new and effective method for the post-harvest preservation of tubers and root crops.
✓ 已验证
DOI
高影响因子
CNS Neuroscience & Therapeutics
·
2022
·Vol. 29
·期 2
·691-711
·
IF 7.035
Jinfang Xu,Jianli Wang,Mingfei Zhao,Chenguang Li,Shen Hong,Jianmin Zhang
Aims Since the inhibitory effect of KNG1 on glioma has been proved, this study further explores the regulation of the lncRNA/miRNA axis on KNG1 in glioma. Methods The miRNAs that target KNG1 and the lncRNA that targets miR-942-5p were predicted by bioinformatics analysis and verified by experiments. The correlations between miR-942-5p and the survival of patients and between KNG1 and miR-942-5p were analyzed. After transfection, cell migration, invasion, proliferation, and cell cycle were detected through wound healing, Transwell, colony formation, and flow cytometry assays. A mouse subcutaneous xenotransplanted tumor model was established. The expressions of miR-942-5p, KNG1, LINC01018, and related genes were evaluated by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), Western blot, or immunohistochemistry. Results MiR-942-5p targeted KNG1, and LINC01018 sponged miR-942-5p. The high survival rate of patients was related to low miR-942-5p level. MiR-942-5p was highly expressed, whereas KNG1 was lowly expressed in glioma. MiR-942-5p was negatively correlated with KNG1. Silent LINC01018 or KNG1 and miR-942-5p mimic enhanced the migration, invasion, and proliferation of glioma cells, and regulated the expressions of metastasis-related and proliferation-related genes. LINC01018 knockdown and miR-942-5p mimic promoted glioma tumor growth in mice. The levels of miR-942-5p and KNG1 were decreased by LINC01018 knockdown, and LINC01018 expression was suppressed by miR-942-5p mimic. MiR-942-5p inhibitor, KNG1, and LINC01018 had the opposite effect to miR-942-5p mimic. Conclusion LINC01018/miR-942-5p/KNG1 pathway regulates the development of glioma cells in vitro and in vivo.
✓ 已验证
DOI
PubMed
高影响因子
Andrology
·
2022
·Vol. 10
·期 4
·808-817
·
IF 4.456
Guotao Chen,Deshui Yu,Yunhong Wu,Jian Dong,Lei Hu,Ninghan Feng
Background The central molecular mechanisms of nonorganic erectile dysfunction remains unknown. Objective This study aimed to investigate the association of dopaminergic neurons projecting to the nucleus accumbens of male rats with nonorganic erectile dysfunction. Materials/methods Nonorganic erectile dysfunction was induced by chronic mild stress. The sucrose consumption test, sexual behavior test, and apomorphine test were carried out to select depression-like rats with erectile dysfunction. These rats were considered as nonorganic erectile dysfunction model rats. Dopamine D1/D2 receptor agonist/antagonist was infused into the nucleus accumbens to observe the effect on sexual behavior. Dopaminergic projections to the nucleus accumbens were labeled with both the retrograde tracer FluoroGold injected into the nucleus accumbens and tyrosine hydroxylase. The expression level of tyrosine hydroxylase in dopaminergic neurons projecting to the nucleus accumbens in the ventral tegmental area was measured. The expression levels of dopamine D1/D2 receptors and tyrosine hydroxylase in the nucleus accumbens were also measured. Results Nonorganic erectile dysfunction was proved by the sucrose consumption test, sexual behavior test, and apomorphine test in model rats. After central infusion of the dopamine D2 receptor agonist into the nucleus accumbens, the recovery of erectile function, sexual arousal, and motivation were indicated by increased intromission ratio and decreased mount latency. Decreased expression levels of dopamine D2 receptors and tyrosine hydroxylase in the nucleus accumbens and decreased expression level of tyrosine hydroxylase in the dopaminergic neurons projecting to the nucleus accumbens were observed in model rats. Discussion These results suggest the impairment of dopaminergic neurons projecting to the nucleus accumbens and dopamine D2 signaling in the nucleus accumbens, causing the suppression of erectile function, sexual arousal, and motivation. Conclusion These results suggest that the impaired dopamine D2 receptor pathway in the nucleus accumbens may be one of the main pathway involved in the development of nonorganic erectile dysfunction in the present model.
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Yuankun Liu,Qi Tao,Xinyu Guo,Jipeng Luo,Jinxing Li,Yongchao Liang,Tingqiang Li
Mineral nutrients play an important role in heavy metal uptake and accumulation in plant. However, the effects of calcium (Ca) supply level on apoplastic transport in roots and consequences for uptake of cadmium (Cd) in hyperaccumulators are poorly understood. Here, we investigated how Ca regulated the development of apoplastic barriers in the roots of two ecotypes of Sedum alfredii and assessed its effects on Cd uptake. Results of correlation analysis indicated that Ca content was positively correlated with the development of Casparian strips (CSs) and suberin lamellae (SL) in the absence or presence of Cd. Simultaneously, low Ca supply was proven to delay the formation of endodermis CSs and suberin accumulation by decreasing the relative expressions of genes associated with CSs localization and lignin/suberin synthesis. Moreover, Cd in apoplastic fluid and cell walls (regarding the apoplastic transport) and symplastic fractions were elevated by low Ca supply. Contrary to high Ca supply, the expression levels of genes related to Cd influx and xylem loading were increased upon low Ca addition in roots of both ecotypes. All the results above suggested that low Ca supply promotes root Cd uptake via apoplastic pathway by delaying apoplastic barriers development and also regulating Cd transport to the xylem in S. alfredii .
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Dongmei He,Xiangyu Zhang,Xiaohong Yao,Yongqiang Yang
Light-triggered antibacterial therapy has been proven to be a secure and effective way to treat bacterial infection. Nevertheless, the long-term security of the common photosensitizer remains to be seen in the body. In this work, carbon dots (CDs) with good biocompatibility are incorporated into TiO 2 nanorods to improve the photocatalytic and photothermal ability of titanium implants under the irradiation of visible light (VL) and near-infrared (NIR) light. The C-TiO 2 NR exhibit excellent in vitro and in vivo antimicrobial effect under 660 nm VL and 808 nm NIR light co-irradiation owing to the combined effect of hyperthermia, reactive oxygen species (ROS) and nanorod structure. Besides, C-TiO 2 NR can improve the adhesion and diffusion of bone marrow mesenchymal stem cells (BMSCs).
✓ 已验证
DOI
PubMed
高影响因子
RNA Biology
·
2021
·
IF 4.652
Yan Wang,Yujie Guo,Xiaolei Zhang,Hui Zhao,Bingbing Zhang,Yi Wu,Jingyu Zhang
Specific miRNA in immune thrombocytopenia (ITP) was screened to explore its intervention effects and mechanisms in ITP. MTT assay and CFSE staining were used to detect the effects of gradient concentrations of thrombopoietin (TPO) on cell proliferation. Expressions of differentially expressed miRNAs were analysed via qRT-PCR in TPO-induced megakaryocytes and ITP plasma. Effects of miR-557 on cell physiological functions were examined by MTT and flow cytometry. Expressions of miR-557, apoptosis-associated genes and Akt/ERK pathways were detected by qRT-PCR and Western blot as needed. Multinucleation of TPO-induced megakaryocytes was determined by megakaryocyte colonies. The toe skin and intestinal bleeding of the ITP rat model were observed and evaluated. Effects of miR-557 on the numbers of platelets, megakaryocytes, and peripheral blood platelets and the expressions of CD4+ T cells, Treg cells, TGF-β, IL-6 and miR-557 in the ITP rats were detected by Giemsa staining, flow cytometry, ELISA and qRT-PCR. MiR-557 was identified as an specific miRNA associated with both ITP and TPO treatment. MiR-557 inhibitor enhanced the physiological functions of TPO-induced megakaryocytes, while miR-557 mimic had the opposite effect. At the molecular level, the expressions of miR-557, cleaved Caspase-3 and Bax were further silenced by inhibitor, on the contrary, the expressions of bcl-2, p-Akt and p-ERK were upregulated. Animal experiments showed that, miR-557 inhibitor increased the numbers of platelets and megakaryocytes, and improved the symptoms of ITP model rats. Our results indicated that miR-557 inhibitor improved ITP by regulating apoptosis-related genes and cellular immunity and activating the Akt/ERK pathway.
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
·
2022
·
IF 4.271
Sen Yan,Sinuo Tian,Zhiyuan Meng,Jin Yan,Ming Jia,Wei Sun,Qiuxia Wang,Jinling Diao,Wentao Zhu,Zhiqiang Zhou
Decabromodiphenyl ethane (DBDPE) as a novel brominated flame retardant is frequently detected in environmental media due to its widespread use. Studies have shown that exposure to environmental pollutants in utero could lead to weight loss in newborns and obesity in adulthood. However, the mechanisms of how the cubs grow rapidly from low birth weight to obesity remain unclear. Although it has been reported that perinatal DBDPE exposure caused obesity in the offspring of mice in adulthood, its metabolic changes in offspring juvenile are unknown. Here, we monitored changes of body weight in cubs following exposure to DBDPE in utero. Furthermore, 1 H NMR-based metabolomics was used to investigate the effects of in utero DBDPE exposure on the metabolic profile of neonates included days 1 and 25. Additionally, the expression levels of key genes in the relevant pathways were quantified. The results showed that exposure to high levels of DBDPE in utero caused cubs to be smaller at birth and grow rapidly during lactation. Metabolomics analysis showed that cubs in the exposed group consistently accelerated glycogenolysis and lipid synthesis to promote appetite and energy absorption, and achieved rapid growth in the subsequent lactation, which was further evidenced by the expression levels of genes related to glycolipid metabolism. These results reveal the mechanism for the first time how the weight loss of newborns in utero environmental pollutant exposure transformed into obesity in adulthood, although the exposures here were much higher than would be anticipated from the environment.
✓ 已验证
DOI
PubMed
高影响因子
POLYMER COMPOSITES
·
2025
·
IF 4.7
Huanhuan Wang,Qi Qu,Jun Yang,Qingbin Tian,Jin Wang,Jiming Gao,Yufeng Liu,Yan He
The practical application of polyamide 6 (PA6) in structural engineering is hindered by its limited toughness and weak interfacial interaction with reinforcing fillers. Carbon nanotubes (CNTs) have been widely explored to overcome these deficiencies, yet their strong tendency to agglomerate and poor compatibility with PA6 restrict efficient load transfer. Herein, we propose a solvent-free one-pot in situ polymerization strategy that simultaneously functionalizes carboxylated CNTs with bis(3-aminopropyl)-terminated polydimethylsiloxane (APDMS) and integrates them into PA6 during ε-caprolactam polymerization. Compared with neat PA6, the nanocomposites exhibited increases of 16.1% in tensile strength, 21.9% in flexural strength, and 10.3% in notched impact strength. These improvements originate from a chemically bridged siloxane interphase, in which APDMS provides dual amino groups for covalent coupling and a flexible backbone for interfacial stress dissipation. The proposed approach eliminates the need for organic solvents and multi-step procedures, providing an environmentally friendly and scalable route to tailor interfacial structures. This work not only demonstrates a balanced enhancement in stiffness, strength, and toughness, but also provides mechanistic insights into interphase engineering for advanced thermoplastic nanocomposites. Highlights Solvent-free one-pot in situ polymerization for CNT/PA6 nanocomposites. Bis(3-aminopropyl)-terminated polydimethylsiloxane (APDMS) enables CNT grafting and PA6 chain growth via dual reactivity. Robust siloxane interphase improves CNT dispersion and stress transfer. Balanced strength and toughness achieved in CNT/PA6 nanocomposites. Interphase reinforcement arises from covalent bonds and stress-dissipating siloxane.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jian Zhu,Qinghao Ning,Tianxing Wang,Zengqi Liu,Long Yu,Hongsheng Liu,Wei Zou
Lignin (Lg), a major dietary phenolic polymer, remains poorly characterized as a substrate for colonic fermentation. We compared an aqueous soluble lignin (SLg) with two less soluble fractions produced by thermal (Lg-200) or alkali-thermal treatment (Lg10–200) processing using an in vitro batch fermentation model. SLg produced modestly higher total short-chain fatty acids (SCFAs) than the other lignin fractions (from ~7.80 to ~8.72 mM), with relatively greater acetate (from ~3.01 to 4.24 mM) and propionate (from ~2.71 to ~3.50 mM), and a pH decrease from 6.60 to 6.15. It also increased microbial diversity and enrichment of taxa linked to carbohydrate fermentation ( e.g. , Ruminococcus torques , Megamonas ), alongside reduced pro-inflammatory Collinsella . Moreover, SLg significantly decreased the Firmicutes/Bacteroidota (F/B) ratio relative to the blank (from ~44.02 to ~30.59, p < 0.05). These results suggest that soluble lignin may influence gut microbial composition and could be further explored as a potential functional ingredient.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL
·
2025
·
IF 7.5
Hugang Li,Hongxu Chen,Yongfei Gao,Yuhong Qin,Shuai Niu,Hui Zhou,Yuanhui Zhang
Crystallization of vivianite-phosphorus is a promising method to recover phosphate as a vital resource from sewage sludge hydrothermal carbonization (HTC) products. This study reveals the in-situ recrystallization mechanism of vivianite-phosphorus (Fe 3 (PO 4 ) 2 ·8H 2 O) regulated by FeCl 3 -assisted HTC in depth. The key finding includes the decipherment that phosphorus is mainly enriched in hydrochar (72.7 ± 2.9 %wt ∼ 84.4 ± 3.31 %wt). Further identified by HRTEM and XRD, dominant phosphorus is profiled as vivianite (Fe 3 (PO 4 ) 2 ·8H 2 O) with very few calcium phosphate crystals (Ca 3 (PO 4 ) 2 ) formation in hydrochar at near-neutral condition. Under acidic conditions, HMF oxidation triggers reductive dissolution of surface-bound Fe(III)-phosphate complexes, releasing Fe 2+ , HPO 4 2− , and PO 4 3− . The anoxic, reducing conditions at circumneutral pH created by HTC, combining with favorable Fe: P ratio (1.72) and lower calcium concentration (532 mg L −1 ), comprehensively drives the crystallization of Fe 3 (PO 4 ) 2 ·8H 2 O. This demonstration-of-practice study provides deep insights into dual organic waste resource and energy valorization.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）