TALANTA
·
2024
·
IF 5.6
Chen Cui,Naxin Liu,Qiurui Nian,Yuntao Xia,Liuchuang Zhao,Leqian Hu,Hongjuan Bai,Dechen Jiang
Improvement in the sensitivity and accuracy of ECL-based biosensors, to a great extent, depends on the fabrication of high-performance electrochemiluminescence (ECL) sensors. Herein, polyethyleneimine (PEI)-grafted bimetallic ZnNi metal-organic framework (ZnNi-MOF/PEI) nanospheres were synthesized to prepare a L012-based ECL immunosensor for detecting carbohydrate antigen 15-3 (CA15-3). The introduced PEI was utilized not only as a bridging element for L012 luminophore and detection antibody (Ab 2 ) immobilization but also as a co-reactant for generating the self-enhanced ZnNi-MOF/PEI-L012@Ab 2 ECL signal probe. Furthermore, Pt nanoparticle-functionalized CeO 2 nanorods (CeO 2 -Pt) featuring high-efficiency electrocatalytic properties, as a co-reaction accelerator, could catalyze H 2 O 2 to generate abundant reactive oxygen species (ROS), further augmenting the ECL signal of ZnNi-MOF/PEI-L012@Ab 2 . In the presence of CA15-3 antigen, the capture antibody (Ab 1 )-conjugated CeO 2 -Pt complex (CeO 2 -Pt@Ab 1 ) and ZnNi-MOF/PEI-L012@Ab 2 would get closer to each other, with the formation of a "signal-on" sandwich-type ECL immunosensor. The ZnNi-MOF-assisted ECL biosensor achieved a detection limit of down to 5.75✕10 -5 U mL -1 . This work shows distinguished potential for measuring low levels of biomarkers that may help in the clinical diagnosis of breast cancer.
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Haolong Tan,Tongwei Gao,Wensheng Yang,Yue Gao,Quan Xie,Yunjun Ruan,Qian Chen,Tinghong Gao
The rapid development of microelectronic devices has increased the demand for miniature energy storage systems that are highly safe and exhibit long cycling life and excellent energy density. However, the lack of low-cost and high-performance fabrication methods presents a significant challenge to thin-film electrode fabrication. This study reports a strategy combining controllable pre-curing treatment with rapid thermal annealing (RTA), which enables in situ fabrication of high-quality sodium vanadium phosphate [Na 3 V 2 (PO 4 ) 3 ] thin films. RTA facilitates the cleavage of weak metal–O bonds in the precursor film, whereas optimisation of the pre-curing duration (0–100 s) effectively eliminates organic residues and strengthens the carbon framework, suppressing particle agglomeration. This synergistic control substantially improves crystallization uniformity and film densification, resulting in a refined grain structure and highly oriented crystal facets. Under optimal conditions, the thin film demonstrates a high specific capacity of 85.6 mAh g −1 at a current density of 1 A g −1 , retains 72.5 mAh g −1 even at 20 A g −1 , and demonstrates 90.3 % capacity retention after 200 cycles. Moreover, the assembled full sodium-ion cell exhibits an average operating voltage of 1.3 V while maintaining 94.2 % capacity retention after 500 cycles. This study elucidates for the first time the synergistic regulation pathway of the pre-curing–RTA coupling mechanism in Na 3 V 2 (PO 4 ) 3 thin-film fabrication and proposes an advanced preparation strategy for cost-effective, highly integrated, high-power sodium-ion thin-film devices, providing critical theoretical foundations and robust technical support for next-generation all–solid state sodium microbattery design and development.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Haina Yuan,Zixiang Zhu,Gongshuai Song,Gongnian Xiao
This study investigated the formation and mitigation of 3-monochloro-1,2-propanediol (3-MCPD) ester in vegetable oils under storage, thermal processing, and chloride supplementation. Prolonged storage (>6 months) markedly accelerated formation (80.8–190 μg/kg·month -1 ), a rate substantially higher than in the initial 6 months (15.2–71.9 μg/kg·month -1 ), underscoring a previously underestimated risk in long-term stored oils even if levels remain below EU limits. Thermal treatment revealed a matrix-dependent dynamic equilibrium, with soybean oil peaking at 220°C (1250 μg/kg) and rapeseed oil at 180°C (997 μg/kg). Among chloride salts, CaCl 2 induced the highest 3-MCPD ester level (2189 μg/kg), revealing the critical, previously detailed role of metal cation properties (coordination capacity, charge density) beyond the mere presence of Cl - . Antioxidants provided dose-dependent mitigation (up to 49% suppression), though their efficacy was limited by thermal stability. Most significantly, mesoporous silica nanoparticles (MSNs) demonstrated exceptional efficacy, achieving a 98% adsorption removal rate of 3-MCPD esters from oil, with a high fitted maximum capacity (Q max = 412.5 μg/g). The combined strategy of antioxidant application followed by MSN treatment offers a novel, practical, and industrially viable solution for significantly reducing 3-MCPD ester exposure in oils and lipid-rich foods.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiang-Yun Lin,Dong-Yang Tian,Zeng-Qiang Wu,Ning Bao
Electrochemical sensors based on molecularly imprinted polymer (MIP) provide an exceptional platform for sensitive and selective analysis of analytes in complex matrix through incubation while in vivo analysis is critical for achieving quantitative information for the biological study in real time. However, the deployment of MIP electrochemical sensors for in vivo analysis highly depends on their forms and fabrication. Here blade-style MIP electrochemical sensors were designed and fabricated in batches based on the technique of lamination, focusing on their applications for in vivo analysis of trace indole-3-acetic acid (IAA) in plants. The blade-style electrochemical sensors were fabricated using stainless steel sheets as supporting and conductive substrates for the working electrodes. The MIP sensors were prepared in batches using cyclic voltammetry (CV) based electropolymerization of pyrrole (the functional monomer) and IAA (the template) followed by elution under a constant potential at a multichannel electrochemical station with improved throughput. After optimization, the sensors could stably and reproducibly quantify IAA with the detection limit of 0.1 μmol·L −1 . The blade-style MIP sensors could be either plugged in tomato fruits for in vivo analysis of IAA or coupled with a piece of hydrogel for non-invasive analysis of IAA in tomato leaves. The blade-style MIP electrochemical sensors may provide a platform for in vivo analysis by not only obviating the time-consuming sample treatment but also simplifying the analytical procedure. This study may pave the way for extensive applications of MIP electrochemical sensors for real-time, spatially resolved analysis of endogenous biomolecules in living organisms in the near future.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Lihua Wu,Wu Lei,Zhen Han,Yuehua Zhang,Mingzhu Xia,Qingli Hao
Poly(3-methylthiophene)/nitrogen doped graphene modified glassy carbon electrode (P3MT/NGE/GCE) was prepared through simple drop-casting of NGE, followed by the electrodeposition of P3MT film for sensitive electrochemical determination of trace amounts of pesticide phoxim. The morphology and structure of P3MT/NGE/GCE were characterized by scanning electron microscopy; the electrochemical behavior of P3MT/NGE/GCE was investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and chronocoulometry. The preparation conditions of modified electrode, the dropping amount of NGE and polymerization laps of P3MT, were optimized. Under the optimized conditions, the reduction peak current of cyclic voltammetric curve varied linearly with the concentration of phoxim over the two linear ranges of 0.02–0.2 μM and 0.2–2.0 μM, and the low detection limit was 6.4 nM according to lower linear range (S/N = 3). The sensor also exhibited excellent selectivity to phoxim over a wide range of possible interferents including ions, biomolecules, common environment pollutants and widely-used organophosphate pesticides. Moreover, the satisfactory practical feasibility of the sensor was evaluated by the environmental phoxim measurements.
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高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2013
·
IF 1.801
Zhong-Ming Yang,Hui Zhu,Juan Sun,Shao-Song Qian,Meng-Na Cai,Hai-Liang Zhu
Five new Cu(II), Zn(II), and Ni(II) 3-(4-nitrophenyl)acrylic acid complexes were synthesized and evaluated for inhibitory activity on jack bean urease. All five complexes were structurally determined by single crystal X-ray analysis. Compared with the positive reference acetohydroxamic acid (IC50 = 13.25 μM), Cu(II) complexes 3 and 4 showed the strongest inhibitory activity against jack bean urease (IC50 = 1.23 and 1.17 μM). Ni(II) and Zn(II) complexes also exhibited inhibitory activities (IC50 = 10.09–13.10 μM).
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 102
·期 13
·5695-5705
·
IF 3.34
Qiao Ying,Cai Hao-Liang,Yang Xi,Zang Yuan-Yuan,Chen Zhi-Gang
In this study, a new method of cryopreservation of lactic acid bacteria (LAB) using natural deep eutectic solvents (NADESs) was developed. Survival rates of LAB during 24-h short-term and 180-day long-term cryostorage at − 20 °C were investigated. The results revealed that survival of Streptococcus thermophilus ( S. thermophilus ) in NADESs after 24 h of cryostorage was superior to survival of two other tested LAB. Moreover, survival was higher at a ratio of NADESs to S. thermophilus of 1:1 ( v / v ) than observed using a 4:1 ( v / v ) ratio. Representative freezing characteristics of five NADESs were elucidated, including thermodynamic properties and hydrogen bonding interactions after addition of water. In order to identify the protective mechanism of NADESs on cell structure and vital metabolic enzymes of S. thermophilus during cryostorage, transmission electron microscopy (TEM) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were employed and enzyme activities of both lactate dehydrogenase (LDH) and β -galactosidase were determined. Subsequently, NADES GlyP prepared from glycerol and L-proline was demonstrated to maintain cell membrane structural integrity and significantly ( p < 0.05) maintain activities of both intracellular enzymes of S. thermophilus . Moreover, NADESs could efficiently penetrate S. thermophilus cells and intracellular β -galactosidase activity could be used to demonstrate NADESs effectiveness in maintaining S. thermophilus survival after long-term cryostorage. These results demonstrate that NADESs can be used as green cryoprotective chemical agents (CPAs) that can efficiently increase LAB viability during cryostorage. These results should have great value in the food production and probiotics industries.
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高影响因子
CELLULOSE
·
2019
·Vol. 26
·期 11
·6755-6768
·
IF 3.917
Zhou Ying,Fu Jing-Jing,Wang Lin-Xiang,Lu Zhisong,Wang Feng,Xia Qingyou,Kang Yuejun,Li Chang Ming,Yu Ling
Abstract A novel free-standing gelatin-impregnated polypropylene nonwoven (gelatin @PP nonwoven hybrid sheet) was prepared by loading gelatin hydrogel on nonwoven fabric, followed by a freeze-drying procedure. Formation of gelatin in the gaps between the PP fibers significantly increased the pore ratio of the scaffolds. In addition, the freeze-drying prepared gelatin enabled fast liquid absorption and rapid swelling. The cell adhesion and cell proliferation assays showed that the gelatin @PP nonwoven hybrid is a ready-to-use substrate for cell-based assays. Cell immunoassay was conducted to measure the protein expression on cells which were cultured on gelatin @PP nonwoven hybrid sheet. In addition, the freeze-drying prepared gelatin @PP nonwoven hybrid sheet was applied for assembling multilayer stacking for 3D cell culture. The ready-to-use convenience of the gelatin @PP nonwoven hybrid was ensured, as the physicochemical properties (chemical group, pore ratio, and swelling rate) and cell compatibility were well preserved after 90 days of storage. Graphic abstract A novel free-standing gelatin-impregnated polypropylene nonwoven (gelatin @PP nonwoven hybrid) was prepared as a ready-to-use substrate for cell culture.
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高影响因子
PSYCHOPHARMACOLOGY
·
2026
·
IF 3.3
Chen Yonglan,Lan Yuxin,Yi Jingyan,Qi Xiaoyi,Liu Li,Liu Sha,Xu Xinxin,Liu Huan,He Yufeng,He Bing,Ma Xiaochi,Lü Muhan,Liang Sicheng
Background Many antidepressants exert their effects by modulating the gut microbiota, but it is unknown if this mechanism extends to the tricyclic antidepressant imipramine. Objective We probed the extent to which specific gut bacterial taxa mediate the antidepressant effects of imipramine. Material and methods Using a chronic unpredictable mild stress (CUMS) mouse model, we administered imipramine, the prebiotic inulin, or their combination, and systematically evaluated depressive-like phenotypes. Gut microbial dynamics were profiled by 16S rRNA sequencing, and fecal microbiota transplantation (FMT) was performed to determine the transmissibility of microbiota-derived antidepressant effects. Immunofluorescence staining was used to quantify brain-derived neurotrophic factor (BDNF) expression and to assess intestinal barrier integrity via ZO-1 and occludin. Results Imipramine treatment significantly increased the relative abundances of putative A. muciniphila and L. reuteri , both of which showed strong correlations with behavioral improvements. Furthermore, FMT from imipramine-treated donors effectively alleviated CUMS-induced depressive-like behaviors, upregulated BDNF expression and restored gut barrier integrity in recipient mice. Co-administration of imipramine and inulin produced synergistic antidepressant effects. Conclusions Imipramine partially exerts its antidepressant activity through microbiota modulation, with putative A. muciniphila and L. reuteri emerging as important mediators. The superior efficacy of the imipramine-inulin combination highlights a promising microbiota-targeted strategy for advancing antidepressant therapy.
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高影响因子
JOURNAL OF MOLECULAR HISTOLOGY
·
2026
·Vol. 57
·期 4
·229
·
IF 2.6
Chen Pei-sheng,Xu Liu-fei,Wang Bo,Zhang Sheng-ping,Luo Yi-yu,Wang Xiang-peng
Glioblastoma (GBM) is a highly malignant brain tumor with a poor prognosis. Long noncoding RNAs (lncRNAs) significantly influence GBM pathogenesis. Although lncRNA MIR4435-2HG is known to promote tumorigenesis in various cancers, its regulatory role in GBM remains undefined. We quantified MIR4435-2HG and miR-181d-5p expression using quantitative reverse transcription polymerase chain reaction. The functional impact of the MIR4435-2HG/miR-181d-5p/MALT1/NF-κB axis was assessed in A172 and U87 MG cells through cell counting kit-8 (CCK-8), wound healing, Transwell, flow cytometry, Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), western blot, and immunofluorescence assays. Interactions between miR-181d-5p, MIR4435-2HG, and MALT1 were confirmed by dual-luciferase reporter, fluorescence in situ hybridization, RNA immunoprecipitation, and RNA pulldown experiments. Finally, a xenograft tumor model in C57BL/6 mice, combined with hematoxylin and eosin staining, immunohistochemistry, and TUNEL staining, was used to investigate the role of MIR4435-2HG in vivo. The results demonstrated that both MIR4435-2HG and MALT1 were upregulated in GBM tissues and cell lines, whereas the expression of miR-181d-5p was reduced. MIR4435-2HG promoted GBM cell proliferation and metastasis in both in vitro and in vivo experiments. Inhibition of MALT1 reversed the promotive effects of miR-181d-5p knock down on GBM cell proliferation and metastasis. Mechanistic studies revealed that MIR4435-2HG upregulates MALT1 expression and activates the NF-κB signaling pathway by sponging miR-181d-5p. In conclusion, MIR4435-2HG overexpression facilitates the malignant progression of GBM through the miR-181d-5p/MALT1/NF-κB pathway.
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高影响因子
MOLECULAR BIOLOGY REPORTS
·
2026
·Vol. 53
·期 1
·1291
·
IF 3.2
Zhu Mo-li,Hao Rui,Zhao Meng-yao,Liu Pei-yao,Zhang Hong-dou,Li Peng,Yin Ya-ling,Liu Hui-jun,Ren Guang-qiu,Zhang Jie,Yang Jing
Background The clinical use of doxorubicin (DOX), a widely used and effective antitumor drug, is limited by its cardiotoxicity. Currently, safe and effective strategies for preventing doxorubicin-induced cardiotoxicity (DIC) remain limited. Therefore, this study aimed to investigate the potential cardioprotective effects and possible underlying mechanisms of L-arginine (L-Arg) against DIC. Methods and Results To investigate the cardioprotective effects and underlying mechanisms of L-Arg, three complementary experimental models were used: male Sprague–Dawley rats ( n = 6 per group), male AMPKα2 knockout (AMPKα2 KO) mice ( n = 6 per group), and H9c2 cardiomyocytes. A DIC model was induced in rats by intraperitoneal injection of DOX (2.5 mg/kg/week) for 6 weeks. Serum nitric oxide (NO) and lactate dehydrogenase (LDH) levels were measured to assess oxidative stress and myocardial injury. Cardiac morphology, inflammation, and fibrosis were evaluated by histological staining and protein expression analyses, whereas miR-29b-3p expression was determined by RT-qPCR. AMPKα2-deficient models and miR-29b-3p gain- and loss-of-function models were used to investigate the underlying mechanisms. Compared with the DOX group, L-Arg significantly improved cardiac function and morphology, reduced oxidative stress, myocardial injury, inflammation, and fibrosis, and increased miR-29b-3p expression (all P < 0.05). Moreover, miR-29b-3p overexpression enhanced, whereas miR-29b-3p inhibition attenuated, the cardioprotective effects of L-Arg ( all P < 0.05 ). These protective effects were also markedly attenuated by AMPKα2 deficiency ( P < 0.05 ). Conclusions L-Arg alleviates DIC by improving cardiac function, reducing oxidative stress, inflammation, and fibrosis, and increasing miR-29b-3p expression. These cardioprotective effects are associated with AMPKα2 activation and enhanced miR-29b-3p expression.
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高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2026
·Vol. 41
·期 4
·1134-1144
·
IF 1.4
Gong Xing,Sun Mingzhi,Wan Pei,Liu Quantao,Wu Shaopeng
This study investigated the crosslinking behavior and curing properties of environmentally friendly polyurethane (PU) modified asphalt (PUMA) prepared by in-situ polymerization. The research aimed to clarify the chemical reaction sites and sequence between the PU prepolymer (PUP) and asphalt components, and to evaluate the microstructural and performance evolution of PUMA during curing. Density functional theory (DFT) calculations were employed to predict reactive sites based on electrostatic potential and frontier molecular orbitals, and to compute Gibbs free energy and transition state energy barriers. The experimental results indicate that phenolic hydroxyl and pyridinic nitrogen in asphaltene molecules and phenolic hydroxyl in resin molecules are the primary sites for reaction with PUP. PUP reacts spontaneously with chain extender (BDO), water, asphaltene, and resin at room temperature, with the reaction following the order: BDO > H 2 O > asphaltene-1 > asphaltene-2 > resin. Microstructural analysis by atomic force microscopy reveals that PU acts as a bridge connecting asphaltene and resin, forming an integrated network that became more perfect with prolonged curing time. Fourier transform infrared spectroscopy of SARA fractions confirms the formation of amide bonds between PUP and asphalt components. The high-temperature performance of PUMA improves with curing time, while the low-temperature performance slightly decreases but still meets specification requirements after 180 days of curing. The findings can provide a fundamental basis for the design and preparation of high-performance PUMA.
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高影响因子
危险品化学品经营许可证（带存储）