3 Biotech
·
2017
·Vol. 8
·期 1
·1-8
·
IF 1.361
Yuan Shengliang,Duan Zhihong,Lu Yingnian,Ma Xiaoli,Wang Sheng
Gracilaria lemaneiformis is mainly used as a raw material source for agar industry, and its extract is rich in natural pigments with antioxidant activities. In this study, a solvent reflux extraction method for decolorization of G. lemaneiformis has been developed in agar production. The extraction conditions were optimized as follows: solvent-to-material ratio, 50:1; ethanol concentration, 70%; number of extractions, 3; extraction time, 0.5 h, under which the total antioxidant yield of the extract reached 2.89 ± 0.88 mg/g dried seaweeds. Their IC 50 values of DPPH radical scavenging activity, hydroxyl radical scavenging activity and superoxide anion scavenging activity were 21.91 ± 1.8 mg/L, 40.59 ± 1.5 mg/L and 160.87 ± 2.8 mg/L, respectively. Further isolation and spectroscopic analysis of natural pigments suggested the strong antioxidant capacities were attributed to chlorophyll derivatives. The results indicate that the decolorization process was able to improve the agar quality, and the extract containing lots of natural pigments had antioxidant activities which may be used in functional food and cosmetics.
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高影响因子
AAPS PHARMSCITECH
·
2012
·Vol. 13
·期 2
·485-492
·
IF 1.432
Shi Jun,Wang Yiming,Luo Guoan
In the present study, we have investigated transdermal administration of ligustrazine phosphate (LP), as an antioxidant, for the treatment of Alzheimer’s disease (AD). The LP transdermal ethosomal system was designed and characterized. Franz-type diffusion cells and confocal laser scanning microscopy were used for the in vitro permeation studies. Furthermore, the effect of LP transdermal ethosomal system on AD was evaluated in the scopolamine-induced amnesia rats by evaluating the behavioral performance in the Morris water maze test. The activities of the antioxidant enzymes and the levels of the lipid peroxidation product malondialdehyde (MDA) in the brain of rats were also determined. The results showed that both the penetration ability and the drug deposition in skin of the LP ethosomal system were significantly higher than the aqueous one. The LP transdermal ethosomal system could recover the activities of the antioxidant enzymes and the levels of MDA in the brain of the amnesic rats to the similar status of the normal rats, which was also indirectly reflected by the improvement in the behavioral performance. In conclusion, LP might offer a potential alternative therapeutic drug in the fight against AD, and ethosomes could be vesicles of choice for transdermal delivery of LP.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 13
·9368-9381
·
IF 2.993
Liu Le,Li Tuanwei,Ruan Zheng,Yan Lifeng
Photodynamic therapy (PDT) combining with near infrared (NIR) imaging is attractive. However, the intrinsic hypoxia in tumor and consumption of oxygen during treatment will decrease PDT. Here an artificial red cell was prepared using polypeptides conjugated hemoglobin as an oxygen carrier. A NIR photosensitizer-brominated 4,4-difluoro-4-bora-3a,a-diaza-s-indacene (BODIPY-Br 2 ) possessing both high fluorescence emission and singlet oxygen generation efficiency was synthesized and also conjugated to polypeptides to achieve NIR imaging-guided PDT. In vitro studies performed on HepG2 cancer cells verified the oxygen carrier, cancer tracing and curing abilities of the as-prepared polymeric nanoparticles. Even under hypoxia condition, it also obviously increases the cell killing rate when exposed light at a low energy (25 mW/cm 2 , 10 min). Meanwhile, the fluorescence of BODIPY in NPs would light up cells for real-time imaging. These results show the potential of the biocompatible and biodegradable P-Hb-B NPs for enhancement of simultaneous tracing and treating of cancer.
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高影响因子
BMC PLANT BIOLOGY
·
2019
·Vol. 19
·期 1
·1-16
·
IF 3.67
Cao Yunpeng,Meng Dandan,Han Yahui,Chen Tianzhe,Jiao Chunyan,Chen Yu,Jin Qing,Cai Yongping
Background Studies have demonstrated that BBX (B-BOX) genes play crucial roles in regulatory networks controlling plant growth, developmental processes and stress response. Nevertheless, comprehensive study of BBX genes in orchids (Orchidaceae) is not well studied. The newly released genome sequences of Dendrobium officinale and Phalaenopsis equestris have allowed a systematic analysis of these important BBX genes in orchids. Results Here we identified 19 ( DoBBX01 – 19 ) and 16 ( PeBBX01–16 ) BBX genes from D. officinale and P. equestris , respectively, and clustered into five clades (I-V) according to phylogenetic analysis. Thirteen orthologous, two DoBBXs paralogous and two PeBBXs paralogous gene pairs were validated. This gene family mainly underwent purifying selection, but five domains experienced positive selection during evolution. Noteworthy, the expression patterns of root, root_tips, stem, leaf, speal, column, lip, and flower_buds revealed that they might contribution to the formation of these tissues. According to the cis-regulatory elements analysis of BBX genes, qRT-PCR experiments were carried out using D. officinale PLBs (protocorm-like bodies) and displayed that these BBX genes were differentially regulated under AgNO 3 , MeJA (Methyl Jasmonate), ABA (abscisic acid) and SA (salicylic acid) treatments. Conclusions Our analysis exposed that DoBBX genes play significant roles in plant growth and development, and response to different environmental stress conditions of D. officinale , which provide aid in the selection of appropriate candidate genes for further functional characterization of BBX genes in plants.
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高影响因子
Nano Research
·
2021
·Vol. 15
·期 2
·1183-1192
·
IF 8.897
Xu Yudi,Ma Sheng,Zhao Jiayu,Si Xinghui,Huang Zichao,Zhang Yu,Song Wantong,Tang Zhaohui,Chen Xuesi
Certain chemo drugs have been reported to potentially induce tumor-specific immune recognition by triggering immunogenic cell death (ICD), which provides a promising alternative way for cancer immunotherapy. However, the immunogenic effects of such treatments are still weak and robust systemic antitumor immune responses are rarely seen when these agents were used alone. Herein, we proposed a trinity immune enhancing nanoparticles (TIENs) for boosting antitumor immune responses of chemo agents. The TIENs was constructed with Food and Drug Administration (FDA) approved polylactic acid (PLA), canonical proton-sponging cationic polymer polyethyleneimine (PEI), and Toll-like receptor 9 (TLR9) agonist cytosine phosphate guanine oligodeoxynucleotide (CpG-ODN). In in vitro studies, the TIENs was proved to (1) promote antigen capturing, (2) antigen-presenting cells (APCs) activation, and (3) antigen cross-presentation. In in vivo studies, intratumorally injected TIENs greatly enhanced antitumor effect and robust immune responses of oxaliplatin and doxorubicin in murine CT26 and 4T1 tumor models, respectively. Furthermore, after decoration with a detachable shielding, the TIENs was proved to be effective in promoting the antitumor effects of chemo agents after intravenous injection. The combination of TIENs with clinically widely used chemo agents should be meaningful in boosting effective antitumor immune responses and cancer therapy.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-19
·
IF 9.429
Peng Xin,Xie Zhuomi,Wang Xiuhua,Zhao Yuxiang,Yang Chuyun,Zhang Zhongyi,Li Mingjie,Zheng Jianping,Wang Yuhui
Background Luminescent nanomaterials (LNMs), especially newly-exploited fluorescent carbon-dots (CDs), have demonstrated promising candidates for sunlight harvesting and enhanced photosynthesis efficiency of crops. However, most of the studies focus on the design and synthesis of LNMs and primary metabolism in biomass acceleration, secondary metabolism that closely associated with the quality ingredients of plants is rarely mentioned. Results UV-absorptive and water-soluble NIR-CDs were harvested via a facile microwave-assisted carbonization method. The effect and regulatory mechanism of NIR-CDs on the secondary metabolism and bioactive ingredients accumulation in Tetrastigma hemsleyanum were explored. A total of 191 differential secondary metabolites and 6874 differentially expressed genes were identified when the NIR-CDs were adopted for enhancing growth of T. hemsleyanum . The phenolic acids were generally improved, but the flavonoids were more likely to decrease. The pivotal differentially expressed genes were involved in biosynthesis of secondary metabolites, flavonoid biosynthesis, porphyrin and chlorophyll metabolism, etc. The gene-metabolite association was constructed and 44 hub genes highly related to quality ingredients accumulation and growth were identified, among which and the top 5 genes of the PPI network might be the key regulators. Conclusion This research provided key regulatory genes and differentially accumulating quality ingredients under NIR-CDs-treatment, which could provide a theoretical basis for expanding the applications of nanomaterial in industrial crop agriculture. Graphical Abstract
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 10
·1-13
·
IF 6.408
Li Xinhao,Li Shengnan,Lv Qiuyan,Wang Chaoxian,Liang Jintao,Zhou Zhide,Li Guiyin
A Golgi protein 73 (GP73) colorimetric biosensor based on the reduced graphene oxide-carboxymethyl chitosan-hemin/platinum@palladium nanoparticles (RGO-CMCS-Hemin/Pt@Pd NPs) with peroxidase-like activity was constructed. The RGO-CMCS-Hemin/Pt@Pd NPs with high peroxidase-like activity were successfully synthesized under mild conditions. Then, the aminylated GP73 aptamer (Apt) was bound to the RGO-CMCS-Hemin/Pt@Pd NPs to form the recognition probe. Another unmodified GP73 aptamer (AptI) was served as the capture probe. In the presence of target GP73, the capture probe and the recognition probe specifically bind to GP73 and form a RGO-CMCS-Hemin/Pt@Pd NP-Apt/GP73/AptI sandwich-type structure, which can oxidase the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB in the presence of H 2 O 2 . GP73 detection was achieved by measuring the peak UV absorption at 652 nm. Under the optimum conditions, the GP73 concentration was linearly related to the absorbance intensity in the range 10.0–110.0 ng/mL, and the limit of detection (LOD) was 4.7 ng/mL. The proposed colorimetric biosensor was successfully applied to detect GP73 in spiked human serum samples with recoveries of 98.2–107.0% and RSDs of 1.90–5.44%, demonstrating the excellent potential for highly sensitive GP73 detection in clinical detection. Graphical abstract A colorimetric biosensor for visual determination of GP73 based on RGO-CMCS-Hemin/Pt@Pd NPs nanozyme with peroxidase-like activity was designed. The GP73 biosensor responses linearly from 10.0–110.0 ng/mL with LOD of 4.7 ng/mL, and shows acceptable specificity and good recovery.
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高影响因子
Science China-Chemistry
·
2021
·Vol. 64
·期 12
·2218-2229
·
IF 9.445
Ye Jiamin,Fu Qinrui,Liu Luntao,Chen Lanlan,Zhang Xuan,Li Qingqing,Li Zhi,Su Lichao,Zhu Rong,Song Jibin,Yang Huanghao
Varieties of contrast agents have been developed for photoacoustic (PA) and ultrasound (US) imaging of cancers in vivo . However, access of traditional contrast agents into the sites of tumors has been principally through passive infiltration without any external force, preventing their deep penetration into the tissues of the tumors, and hindering the use of PA and US for deep tumor imaging. The concept of micro/nanomotors has been the focus of increasing attention as active theranostic agents due to their active movement in particular fluids, thereby conducting assigned tasks. Herein, US-propelled Janus mesoporous SiO 2 partially coated gold nanorods (Au NR-mSiO 2 ) were fabricated for deep tumor NIR-II PA imaging and synergistic sonodynamic-gas therapy. Following US irradiation, 2,2-azobis[2-(2-imidazolin-2-yl) propane] dihydrochloride (AIPH) loaded in mSiO 2 (Au NR-mSiO 2 /AIPH) generated N 2 microbubbles with high efficiency to achieve nanomotor drive. Due to the deep penetration of US, the nanomotors exhibited a capability to travel deep within sites of tumors, providing enhanced PA/US imaging inside the tumors. Furthermore, the nanomotor based cancer therapy was demonstrated through synergistic N 2 gas and sonodynamic therapy. The US-propelled nanomotors demonstrated a novel strategy for the simultaneous PA/US dual imaging deep within tumor tissues and precise therapy of large tumors.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 14
·10013-10024
·
IF 2.993
Ban Qingfu,Zhuang Qiang,Su Kehe,Wu Si,Kong Jie
The existence of toxic Cu ions in the products synthesized by Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) click chemistry hinders biomedical applications of these products. Cu ions are difficult to remove because stable Cu(II)–triazole coordination complexes are formed in the CuAAC products. To address this problem, we demonstrate a novel interfacial liquid phase-driven method for the removal of Cu ions. We synthesized small molecules, linear polymers, and hyperbranched polymers by using CuAAC. To remove Cu ions in these products, Cu ions were precipitated using Na 2 S in a solvent mixture that contains water, dimethylformamide, and dichloromethane (DCM). The products and the CuS precipitates were automatically dispersed in the DCM phase and aqueous phase, respectively, resulting in separation of the products and CuS. Compared to the conventional Cu removal methods, our new method is highly efficient, fast, and generally applicable to small molecules and polymers. More than 98% Cu in the CuAAC products can be removed by using our method. Moreover, we tested the biocompatibility of hyperbranched polymers synthesized using CuAAC and purified using our method. These polymers are compatible to cells, indicating our Cu removal method shows promise in purification of biomaterials synthesized using CuAAC.
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高影响因子
Frontiers of Environmental Science & Engineering
·
2021
·Vol. 16
·期 8
·1-14
·
IF 4.357
Luo Jingyang,Fang Shiyu,Huang Wenxuan,Wang Feng,Zhang Le,Fang Fang,Cao Jiashun,Wu Yang,Wang Dongbo
Surfactants were expected to exhibit positive effects on the waste activated sludge (WAS) disposal. However, the systematic comparison of different categories of surfactants on the WAS fermentation and the functional mechanisms, especially microbial metabolic traits, have not yet been precisely explored. This study revealed the positive effects of different surfactants on the volatile fatty acid (VFA) production, which followed the order of alkyl polysaccharides (APG)>sodium dodecylbenzene sulfonate (SDBS)>hexadecyl trimethyl ammonium bromide (HTAB). Mechanistic exploration found that the presence of different surfactants improved solubilization and hydrolysis steps, and then contributed to the subsequent acidification with different efficiencies. The functional microorganisms associated with VFA generation were enriched in surfactant-conditioned reactors. Metagenomic analysis further indicated that the key genes involved in the particular process of VFA generation were over-expressed. The simultaneous bioavailable substrate improvement, functional bacterial enrichment, and metabolic activity upregulation induced by different surfactants jointly contributed to VFA promotion during WAS fermentation. This study could provide a comprehensive realization of surfactants’ impacts on the WAS fermentation process, and more importantly, it reminded the public to discern the distinct interplaying effects induced by different chemicals in regulating the WAS disposal and resource recovery.
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高影响因子
MOLECULAR BIOLOGY REPORTS
·
2026
·Vol. 53
·期 1
·1702
·
IF 3.2
Lan Xiaoxu,Sun Hang,Mu Yulong,Hou Xiaoqing,Ti Chenhui,Sun Weimiao,Xie Shuyang,Li Youjie,Sun Yunxiao
Background Imatinib (IM) resistance poses a major unresolved clinical challenge in chronic myeloid leukemia (CML) treatment, significantly compromising patient prognosis. This study aims to elucidate the role of cell division cycle-associated protein 4 (CDCA4) in IM resistance and the underlying molecular mechanisms in CML. Methods and Results Pan-cancer analysis revealed that high CDCA4 expression was significantly associated with shorter overall survival in multiple cancer types. Quantitative real-time PCR demonstrated upregulated CDCA4 expression in both peripheral blood samples from CML patients and IM-resistant cell lines. CCK-8 assays showed that CDCA4 overexpression markedly enhanced IM resistance and promoted cell proliferation in CML cells. Mechanistic investigations indicated that CDCA4 is associated with hyperactivation of the AKT-mTOR signaling axis and concurrent suppression of basal autophagy, a phenotype that correlates with imatinib chemoresistance in CML cells. Subsequent molecular profiling identified a circ_0029325-dependent regulatory circuit that governs CDCA4 expression levels. Conclusions Our findings clarify the critical function of CDCA4 in CML IM resistance via the AKT-mTOR/autophagy axis and reveal its upstream circ_0029325 regulatory mechanism. This work provides promising potential biomarkers and therapeutic targets for improving CML treatment outcomes.
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高影响因子
Nature Photonics
·
2026
·
IF 38.1
Qiu Jian,Huang Yuling,Wong Pui Ying,Chang Kin Ting,Xue Jie,Chen Zhongwei,Wang Zhiyu,Yao Jingwen,Yu Zhi-Gang,Chen Xihan,Lu Haipeng
The promises of halide perovskites for chiroptical and spintronic applications hinge largely on how long their spin coherence can last. Recent demonstrations of spin injection from chiral single quantum wells to achiral semiconductors have enabled a variety of spin functionalities. However, their spin polarization at the device level remains limited due to rapid interfacial and bulk spin losses. Here we demonstrate a spin cascade strategy by constructing oriented perovskite chiral multiple quantum wells to extend the spin lifetime. In chiral multiple quantum wells, quantum wells with varying well widths exhibit intrinsic and continuous chirality across all quantum-well phases with an average circular dichroism dissymmetry factor of 1.5 × 10 −2 . In this system, slowly energy-varying and spatially coupled chiral multiple quantum wells establish consecutive intermediate polarization states for spin cascade, thereby extending the spin coherence lifetime to ~5 ns at room temperature. The long-lived spin coherence yields circularly polarized luminescence with a luminescence dissymmetry factor (| g lum |) of 1.4 × 10 −2 and spin-dependent charge transport with a polarization degree of over 80%, enabling efficient X-ray-triggered dissymmetric photopolymerization and high-performance circularly polarized light photodetectors. This work opens the way to long-lived spin coherence in halide perovskites, promoting their use in chiroptical and spintronic devices.
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