Forests
·
2025
·Vol. 16
·期 12
·1854
·
IF 2.5
Lijie Wang,Honggang Sun,Jianfeng Zhang,Linshui Dong
Chinese fir (Cunninghamia lanceolata) is a cornerstone timber species in southern China. However, yet its plantation productivity frequently declines under successive rotations, threatening long-term sustainability. While belowground processes are suspected drivers, the mechanisms—particularly plant–soil–microbe interactions—remain poorly resolved. To address this, we examined a chronosequence ofC. lanceolataplantations (5, 15, 20, and 30 years) in Jingdezhen, Jiangxi Province, integrating soil physicochemical assays, high-throughput sequencing, and extracellular enzyme activity profiling. We found that near-mature stands (20 years) exhibited a 60.7% decline in mean annual volume increment relative to mid-aged stands (15 years), despite continued increases in individual tree volume—suggesting a strategic shift from resource-acquisitive to nutrient-conservative growth. Peak values of soil organic carbon (32.87 g·kg−1), total nitrogen (2.51 g·kg−1), microbial biomass carbon (487.33 mg·kg−1), and phosphorus (25.65 mg·kg−1) coincided with this stage, reflecting accelerated nutrient turnover and intensified plant–microbe competition. Microbial communities shifted markedly over time: Basidiomycota and Acidobacteria became dominant in mature stands, replacing earlier Ascomycota and Proteobacteria. Random Forest and Partial Least Squares Path Modeling (PLS-SEM) identified total nitrogen, ammonium nitrogen, and total phosphorus as key predictors of productivity. PLS-SEM further revealed that stand age directly enhanced productivity (β = 0.869) via improved soil properties, but also indirectly suppressed it by stimulating microbial biomass (β = 0.845)—a “dual-effect” that intensified nutrient competition. Fungal and bacterial functional profiles were complementary: under phosphorus limitation, fungi upregulated acid phosphatase to enhance P acquisition, while bacteria predominately mediated nitrogen mineralization. Our results demonstrate a coordinated “soil–microbe–enzyme” feedback mechanism regulating productivity dynamics inC. lanceolataplantations. These insights advance a mechanistic understanding of rotation-associated decline and underscore the potential for targeted nutrient and microbial management to sustain long-term plantation yields.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-13
·
IF 10.435
Niu Yuqing,Galluzzi Massimiliano,Fu Ming,Hu Jinhua,Xia Huimin
One of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia. The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model. Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method. The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood. Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency. At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery. This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction. Graphical Abstract
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 21
·13506
·
IF 6.208
Guobin Li,Lan Zhang,Zhe Lu,Baoqiang Yang,Hui Yang,Peng Shang,Jean X. Jiang,Dong’en Wang,Huiyun Xu
Osteoporosis and sarcopenia (termed “Osteosarcopenia”), the twin-aging diseases, are major contributors to reduced bone mass and muscle weakness in the elderly population. Connexin 43 (Cx43) in osteocytes has been previously reported to play vital roles in bone homeostasis and muscle function in mature mice. The Cx43-formed gap junctions (GJs) and hemichannels (HCs) in osteocytes are important portals for the exchange of small molecules in cell-to-cell and cell-to-extracellular matrix, respectively. However, the roles of Cx43-based GJs and HCs in both bone and muscle aging are still unclear. Here, we used two transgenic mouse models with overexpression of the dominant negative Cx43 mutants primarily in osteocytes driven by the 10-kb Dmp1 promoter, R76W mice (inhibited gap junctions but enhanced hemichannels) and Δ130–136 mice (both gap junction and hemichannels are inhibited), to determine the actions of Cx43-based hemichannels (HCs) and gap junctions (GJs) in the regulation of bone and skeletal muscle from aged mice (18 months) as compared with those from adult mice (10 months). We demonstrated that enhancement of Cx43 HCs reduces bone mass due to increased osteoclast surfaces while the impairment of Cx43 HCs increases osteocyte apoptosis in aged mice caused by reduced PGE2levels. Furthermore, altered mitochondrial homeostasis with reduced expression of Sirt-1, OPA-1, and Drp-1 resulted in excessive ROS level in muscle soleus (SL) of aged transgenic mice. In vitro, the impairment of Cx43 HCs in osteocytes from aged mice also promoted muscle collagen synthesis through activation of TGFβ/smad2/3 signaling because of reduced PGE2levels in the PO CM. These findings indicate that the enhancement of Cx43 HCs while GJs are inhibited reduces bone mass, and the impairment of Cx43 HCs inhibits PGE2level in osteocytes and this reduction promotes muscle collagen synthesis in skeletal muscle through activation of TGFβ/smad2/3 signaling, which together with increased ROS level contributes to reduced muscle force in aged mice.Keywords:osteocytes;hemichannels;gap junctions;bone-muscle crosstalk;aging
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Journal of Materials Chemistry B
·
2012
·Vol. 1
·期 3
·284-292
·
IF 0
Pengfei Gou,Wenwen Liu,Weiwei Mao,Jianbin Tang,Youqing Shen,Meihua Sui
The clinical utility of doxorubicin (DOX) is restricted by its severe side effects. Continuous efforts are aimed at developing efficacious DOX-delivery systems that may overcome the drawbacks of existing ones. Herein, we report a self-assembling prodrug forming high drug loading nanoparticles for DOX delivery. A low molecular weight polyethylene glycol (PEG) chain as the hydrophilic part was anchored to hydrophobic DOX via an acid-cleavable hydrazone bond to form the amphiphilic prodrug PEG–DOX. In aqueous solution, PEG–DOX formed nanoparticles with a diameter of ∼125 nm and extremely high drug loading (∼46 wt%). These nanoparticles were stable in PBS but released DOX in an acidic pH-triggered manner. Interestingly, taken up by cells via endocytosis, PEG–DOX bypassed the P-glycoprotein (P-gp)-mediated efflux of DOX, leading to drug accumulation in DOX-resistant human breast cancer cells (MCF-7/ADR). More importantly, PEG–DOX exhibited potent antitumor activity in vitro and in vivo, and showed significantly increased in vivo safety than free DOX. These encouraging data merit further preclinical and clinical development on PEG–DOX.
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Oncology Letters
·
2018
·Vol. 16
·期 2
·2289-2298
·
IF 1.664
Chunyu Li,Qi Wang,Shen Shen,Xiaolu Wei,Guoxia Li
Metastasis is the primary cause of mortality in patients with breast cancer and lacks effective therapeutic agents. Oridonin, an active diterpenoid compound isolated from Rabdosia rubescens, was identified to be the most potent anti‑tumor ingredient. However, the molecular mechanisms responsible for its anti‑metastatic effects remain unclear. In the present study, oridonin significantly suppressed the migration, invasion and adhesion of MDA‑MB‑231 and 4T1 breast cancer cells, and inhibited tube formation of human umbilical vein endothelial cells in a dose‑dependent manner. The expression levels of epithelial‑mesenchymal transition (EMT)‑associated marker and the hypoxia inducible factor 1α (HIF‑1α)/vascular endothelium growth factor (VEGF) signaling pathway mRNA and proteins were determined by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively in vitro. The results demonstrated that oridonin effectively inhibited EMT as demonstrated by the significant increases in the expression levels of E‑cadherin, and decreased expression of N‑cadherin, Vimentin and Snail. In addition, oridonin exerted its anti‑angiogenesis activity through significantly decreasing HIF‑1α, VEGF‑A and VEGF receptor‑2 protein expression. Furthermore, oridonin was demonstrated to decrease the micro‑vessel density as evidenced by the decreased expression of cluster of differentiation 31, a marker for neovasculature. In brief, oridonin inhibits tumor cell migration, invasion and adhesion, as well as tumor angiogenesis, which are mediated by suppressing EMT and the HIF‑1α/VEGF signaling pathway. The results of the present study suggest that oridonin may be a promising anti‑metastatic agent in breast cancer treatment.
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Regenerative Biomaterials
·
2025
·
IF 8.1
Huang Can,Li Jie,Aou Xueyimu,He Hongwei,Chu Yun,Gao Jianping,Le Hao,Wang Xinhui,Sun Huilin,Wang Huimin,Zhu Ziqing,Fan Yujiang,Zhang Guifeng,Fan Xiaoju,Yuan Tun
Protein-based biomaterials, particularly collagen-derived materials, have been widely applied in medical devices and regenerative medicine due to their excellent biocompatibility and tissue repair-promoting functions. However, the degradation of these materials in vivo may trigger immune responses and other physiological reactions, especially through interactions between degradation products and the immune system. To better evaluate their safety and efficacy, particularly regarding the potential immunotoxicological risks posed by degradation products, this study proposes a comprehensive evaluation framework combining degradation product simulation, immunotoxicological assessment, and chronic toxicity testing, aiming to more fully identify potential risks during the degradation process. This study first simulated the degradation process in vitro, analyzing the compatibility of the resulting degradation products with human proteins to reveal potential molecular interaction risks. Based on this, a systematic immunotoxicological evaluation was conducted from multiple dimensions, including complement activation, humoral immunity, cellular immunity, and inflammatory responses, to thoroughly assess the immunological safety of the material. Furthermore, chronic toxicity experiments confirmed the long-term biosafety and stability of the material. The results demonstrate that the established comprehensive evaluation framework provides new methodological support and reference for the in vivo long-term biological risk assessment. Using this framework, recombinant type III collagen (RC) was found to exhibit favorable biosafety and immunological compatibility at the molecular, immune, and systemic toxicity levels.
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Applied Materials Today
·
2020
·
IF 8.352
Zhichao Hu,Qian Tang,Deyi Yan,Gang Zheng,Mingbao Gu,Zucheng Luo,Cong Mao,Zhiyong Qian,Wenfei Ni,Liyan Shen
Among metabolic and systemic bone diseases, osteoporosis is known as one of the most progressive disease characterized by bone microstructural deterioration and bone mass reduction. Calcitriol (Cal) is widely accepted as a fat-soluble small molecule drug with reported anti-osteoporotic activity. However, delivery systems capable of local sustained release of small molecule drugs remain a significant challenge. Herein, based on poly(D, L-lactide)-poly(ethylene glycol)-poly(D, L-lactide) (PDLLA-PEG-PDLLA) hydrogels, we developed a thermosensitive and multifunctional sustainable delivery system by doubly integrating with polydopamine modified nano-hydroxyapatite (HA-D) and calcitriol-loaded amino-terminated poly(ethylene glycol)-block-poly(epsilon-caprolactone) (PCL-PEG-NH 2 ) micelles to realize the long-term delivery of small molecule drugs. The sustained release of Cal from the system was associate with triple controlled release effects including the protective screen of the hydrogels over micelles, the reservoir effect of the HA nanoparticles for Cal, and the encapsulation of Cal in micelles. In vitro , results from the present study demonstrated that sustainable delivery system effectively activated osteogenesis, proliferation and migration in these bone mesenchymal stromal cells of ovariectomized rats (OVX-rBMSCs). In vivo , the composite Cal-loaded system was verified to promote bone regeneration in the critical-size femoral epicondyle bone defect model. Meanwhile, little cytotoxicity and appropriate degradation rate of the composite Cal delivery system were revealed. Thus, incorporating Cal into a multifunctional delivery system to perform a long-acting drug delivery in an injectable hydrogel form is a promising therapeutic to improve anti-osteoporotic ability and promote bone regeneration of osteoporosis.
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BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
·
2020
·
IF 4.352
Na Niu,Xu Yang,Bao-Long Zhang,Chen Liang,Di Zhu,Qiu-Shi Wang,Yong-Xu Cai,Yan-Chao Yang,Xue Ao,Ming-Ming Wu,Zhi-Ren Zhang
We previously showed that increased epithelial sodium channel (ENaC) activity in endothelial cells induced by oxidized low-density lipoprotein (ox-LDL) contributes to vasculature dysfunction. Here, we investigated whether ENaC participates in the pathological process of atherosclerosis using LDL receptor-deficient (LDLr −/− ) mice. Male C57BL/6 and LDLr −/− mice were fed a normal diet (ND) or high fat diet (HFD) for 10 weeks. Our data show that treatment of LDLr −/− mice with a specific ENaC blocker, benzamil, significantly decreased atherosclerotic lesion formation and expression of matrix metalloproteinase 2 (MMP2) and metalloproteinase 9 (MMP9) in aortic arteries. Furthermore, benzamil ameliorated HFD-induced impairment of aortic endothelium-dependent dilation by reducing expression of proinflammatory cytokines, including TNF-α, IL-1β, and IL-6 and production of adhesion molecules including VCAM-1 and ICAM-1 in both C57BL/6 and LDLr −/− mice fed with HFD. In addition, HFD significantly increased ENaC activity and the levels of serum lipids, including ox-LDL. Our in vitro data further demonstrated that exogenous ox-LDL significantly increased the production of TNF-α, IL-1β, IL-6, VCAM-1 and ICAM-1. This ox-LDL-induced increase in inflammatory cytokines and adhesion molecules was reversed by γ-ENaC silencing or by treatment with the cyclooxygenase-2 (COX-2) antagonist celecoxib. Benzamil inhibited HFD-induced increase in COX-2 expression in aortic tissue in both C57BL/6 and LDLr −/− mice, and γ-ENaC gene silencing attenuated ox-LDL-induced COX-2 expression in HUVECs. These data together suggest that HFD-induced activation of ENaC stimulates inflammatory signaling, thereby contributes to HFD-induced endothelial dysfunction and atherosclerotic lesion formation. Thus, targeting endothelial ENaC may be a promising strategy to halt atherogenesis.
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Current Drug Delivery
·
2022
·Vol. 19
·期 3
·347-356
·
IF 3.758
Yin Fangming,Meng Shu,Zhao Xin,Wang Huining,Ning Yingkai,Li Yangdulin,Chen Zaixing
Background: Oxaprozin is labeled as a Class II drug in the biopharmaceutical classification system, and its poor solubility in the entire gastrointestinal tract may be the main reason for its insufficient oral absorption capacity. Objective: The purpose of this study was to develop
an oxaprozin formulation to enhance its oral absorption. Methods: Oxaprozin-loaded microemulsions were prepared using the titration method and pseudoternary phase diagram. Characterization experiments were performed on microemulsion preparations, including pH, particle size, shape, zeta
potential, and stability (thermodynamic, dilution, and differential scanning calorimetry). Then, the in vitro release of the microemulsion and in vivo pharmacokinetics in rats were evaluated. Results: Several microemulsion formulations were prepared. The optimal formulation was 15% oleoyl
macrogolglycerides, 35% Tween 20/isopropanol (Km=2), and 50% distilled water. Its particle size met the requirements, and it had a spherical shape with a negatively charged surface. This microemulsion-loaded drug was applied to in vitro release and in vivo pharmacokinetic experiments at 7.47
mg/mL. In vitro release of the oxaprozin-loaded microemulsion best fit the firstorder model, while the microemulsion preparation had a certain sustained-release effect. In vivo pharmacokinetic experiments indicated that the microemulsion formulation significantly delayed the peak time of the
blood concentration and simultaneously prolonged the half-life of drug elimination. Conclusion: The obtained data revealed satisfactory results for this novel microemulsion of oxaprozin, which is very meaningful for clinical trials.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
·
IF 4.006
Ben Niu,Ping Shao,Simin Feng,Dan Qiu,Peilong Sun
Beta-carotene has been used as a food colorant and functional ingredient, but these applications are limited due to its poor stability. The aim of this work was to improve the stability of β-carotene by encapsulating it in electrosprayed particles. The electrohydrodynamic behavior of pullulan solution was studied and the influence of encapsulation and core-to-wall ratio on the stability of β-carotene at different temperatures and relative humidity were investigated. The specific viscosity of the polymer solution increased linearly with the concentration until 5% w/w concentration. The critical entanglement concentration of the pullulan was found to be 4.58% wt. Among the various concentrations of pullulan studied, 3% w/w pullulan solution yielded spherical, monodisperse nanoparticles. Electrosprayed particles with 1:50 core-to-wall ratio had highest encapsulation efficiency. The rate of β-carotene loss displayed first order reaction kinetic within 14 days, while displayed diffusion limited mode after 14 days storage. The retention of β-carotene from the electrosprayed particles accelerated markedly with decreasing temperatures and relative humidity. This study provides a strategy for the preparation of promising electrosprayed particles to improve the stability of β-carotene.
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Cells
·
2021
·Vol. 10
·期 4
·738
·
IF 6.6
Michael Edbert Suryanto,Gilbert Audira,Boontida Uapipatanakul,Akhlaq Hussain,Ferry Saputra,Petrus Siregar,Kelvin H.-C. Chen,Chung-Der Hsiao
Antidepressants are well-known drugs to treat depression and major depressive disorder for humans. However, the misuse and abuse of antidepressants keep increasing with several side effects reported. The aim of this study was to assess the potential adverse effects of 18 antidepressants by monitoring zebrafish larval locomotor activity performance based on the total distance traveled, burst movement count, and total rotation count at four dark-light intercalated phases. In general, zebrafish larvae displayed sedative effects after antidepressant exposure by showing a significant reduction in all of the locomotor activity-related endpoints. However, three antidepressants i.e., amitriptyline, amoxapine, and sertraline were able to trigger a significantly high locomotor activity in zebrafish larvae during the light cycle. These differences might be due to the pharmacologic differences among the antidepressants. In addition, since each antidepressant possesses a different dosage range from the other, overdoses of these antidepressants might also be the causes of these differences. Furthermore, based on these results, a further study was conducted to observe the effect of these three antidepressants in lower concentrations. From the results, biphasic effects in terms of zebrafish larval locomotor activity were demonstrated by these drugs. Even though further studies are still required to validate the mechanism, these findings indicate that these antidepressants might share a common mechanism responsible for their effects on zebrafish larval locomotor activity although there were some differences in potency of these effects.Keywords:zebrafish larvae;antidepressants;behavior;locomotion;phenomics;biphasic effect
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Yinfang Yan,Lin Li,Mingyuan Zheng,Bingbing Duan,Jiajing Zhang,Yashan Li,Wei Liu,Xu Liu
BACKGROUND Spine grapes are widely cultivated in southern China because of their strong adaptability to hot and humid climates. As a wild species native to China, spine grape ( Vitis davidii Foëx) was studied as a resource of proanthocyanidins (PAs). PA composition, biosynthesis, and histochemistry in different tissues (skins, seeds, and stems) during berry development were analyzed in this study. RESULTS The findings revealed that PA accumulation occurred in concurrence with flowering and was completed by veraison. High-performance liquid chromatographic results showed that the epicatechin type was the most dominant. The skins were more likely to accumulate PA polymers. Reverse transcription–quantitative polymerase chain reaction analysis revealed that the expression levels of structural genes (flavonoid-3′-hydroxylase, flavonoid-3′5′-hydroxylase, dihydroflavonol 4-reductase, leucoanthocyanidin reductase, and leucoanthocyanidin dioxygenase) were positively associated with PA dynamic changes. Histochemical results revealed that PAs in skins were mainly found in the hypodermis of the exocarp, PAs in seeds were mainly found in the middle layer of the outer integument of the testa, and PAs in stems were mainly found in the phloem. CONCLUSION This study provides a clear understanding of the spatial and temporal accumulation of PAs in spine grape, and forms a basis for the analysis of structural profiles and synthesis of PAs and their biological effects. © 2024 Society of Chemical Industry.
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