BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Yunlei Zhou,Huanshun Yin,Xue Li,Zhi Li,Shiyun Ai,Hai Lin
A sensitive and selective electrochemical biosensor was fabricated for protein kinase A (PKA) activity assay. Multiple signal amplification techniques were employed including the nanocomposite of gold nanoparticles and carbon nanospheres ( [email protected] ), the biocomposite of SiO 2 and streptavidin (SiO 2 -SA), the composite of AuNPs and biotinylated β -galactosidase (AuNPs-B-Gal) and in situ enzymatic generation of electrochemical activity molecule of p -aminophenol. After peptides were assembled on [email protected] modified electrode surface , they were phosphorylated by PKA in the presence of ATP . Then, biotinylated Phos-tag was modified on electrode surface through the specific interaction between Phos-tag and phosphate group . Finally, SiO 2 -SA and AuNPs-B-Gal were captured through the specific interaction between biotin and streptavidin. Because the electrochemical response of p -aminophenol was directly related to PKA concentration, an innovative electrochemical assay could be realized for PKA detection. The detection limit was 0.014 unit/mL. The developed method showed high detection sensitivity and selectivity . In addition, the fabricated biosensor can be also applied to detect PKA in human normal gastricepithelial cell line and human gastric carcinoma cell line with satisfactory results.
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NEW JOURNAL OF CHEMISTRY
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2016
·Vol. 40
·期 8
·6955-6961
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IF 3.277
Juanjuan Xu,Quanhong Ying,Qiao Xia,Ni Wang,Xia Lin,Yingzi Fu
The HS-β-cyclodextrin/gold nanoparticles/hollow carbon microspheres (HS-β-CD/AuNPs/HCMS) hybrids were successfully synthesized and characterized via scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A simple and reliable chiral sensing platform constructed from the prepared hybrids was used for enantioselective recognition of ascorbic acid (AA) and isoascorbic acid (IAA). Moreover, the stereoselectivity of HS-β-CD/AuNPs/HCMS to AA or IAA was investigated via differential pulse voltammetry (DPV). The results showed obvious differences in the peak currents of AA and IAA, demonstrating that this strategy could be employed to enantioselectively recognize AA and IAA. Under the optimum conditions, the chiral sensor exhibited an acceptable linear response to AA or IAA in the linear range of 1.0 × 10−4 to 5.0 × 10−3 M with a limit of detection of 1.7 × 10−5 M (S/N = 3). This approach provided a new available sensing interface to recognize and determine AA or IAA by electrochemical technology.
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JOURNAL OF CHROMATOGRAPHY A
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2016
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IF 3.926
Jialiang Guo,Yuan Xiao,Yuanjing Lin,Jacques Crommen,Zhengjin Jiang
Low column efficiency for enantioseparations in capillary liquid chromatography (CLC) is a major problem commonly encountered with β -cyclodextrin ( β -CD) functionalized polymer-based monoliths. In order to investigate the effect of the crosslinker type on enantioseparation performance, three commonly used crosslinkers, i.e. 1,4-bis(acryloyl)piperazine (PDA), ethylene dimethacrylate (EDMA) and N , N -methylenebisacrylamide (MBA), were copolymerized using the one-pot approach with glycidyl methacrylate-mono-6-amino-6-deoxy- β -CD (GMA-NH 2 - β -CD) as functional monomer. The three monolithic columns, including poly(GMA-NH 2 - β -CD- co -PDA) and poly(GMA-NH 2 - β -CD- co -MBA), as well as the previously reported column poly(GMA-NH 2 - β -CD- co -EDMA) were systematically compared with respect to morphology, permeability, β -CD density, retention mechanism and efficiency. The enantioseparation ability of each column was evaluated using 14 chiral compounds, including mandelic acid derivatives, profens, N -derivatized amino acids, and herbicides, as test substances. The β -CD-functionalized monolith with MBA as crosslinker was found to exhibit higher polarity, higher column efficiency and better enantioseparation performance than those with PDA or EDMA as crosslinker.
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Frontiers of Materials Science
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2017
·Vol. 11
·期 3
·223-232
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IF 1.471
Wei Lulu,Lu Beibei,Li Lei,Wu Jianning,Liu Zhiyong,Guo Xuhong
A novel β-cyclodextrin-poly(2-(2-methoxyethoxy)ethyl methacrylate)- co -poly(ethylene glycol) methacrylate (abbreviated as: β-CD-(P(MEO 2 MA- co -PEGMA)) 21 ) was prepared by using the one-step strategy, and then the star-shaped copolymers were used in the atom transfer radical polymerization (ATRP). The structure of star-shaped β-CD-(P(MEO 2 MA- co -PEGMA)) 21 copolymers were studied by FTIR, 1 H NMR and gel permeation chromatography (GPC). The star-shaped copolymers could self-assembled into micelles in aqueous solution owing to the outer amphiphilic β-CD as a core and the hydrophilic P(MEO 2 MA- co -PEGMA) segments as a shell. These thermo-responsive starshaped copolymers micelles exhibited lower critical solution temperature (LCST) in water, which could be finely tuned by changing the feed ratio of MEO 2 MA to PEGMA. The LCST of star-shaped β-CD-(P(MEO 2 MA- co -PEGMA)) 21 copolymer micelles were increased from 35°C to 58°C with the increasing content of PEGMA. The results were investigated by DLS and TEM. When the temperature was higher than corresponding LCSTs, the micelles started to associate and form spherical nanoparticles. Therefore, β-CD-(P(MEO 2 MA- co -PEGMA)) 21 star-shaped copolymer micelles could be potentially applied in nano-carrier, nano-reactor, smart materials and biomedical fields.
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JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
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2017
·Vol. 56
·期 2
·203-212
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IF 2.952
Hai-Chao Huang,Zong-Jun Li,Bin Wang,Xiaolu Chen,Yue-Sheng Li
A series of novel aluminum complexes containing bulky aryl-β - ketiminato ligands [ArNCH C 10 H 7 C 6 H 5 O]Al(CH 3 ) 2 ( 3a , Ar = C 6 F 5 ; 3b , Ar = C 6 H 5 ; 3c , Ar = 2,6- i Pr 2 C 6 H 3 ) have been synthesized in high yields. These complexes were identified by 1 H and 13 C NMR spectroscopy, elemental analysis, and X - ray structural analysis. All the aluminum complexes could efficiently catalyze the ROP of ɛ- caprolactone ( ɛ -CL) and Lactide (LA) in a controlled manner. It was found that the steric effect of the ligand has less effect on the ROP of CL, while the polymerization rate of L -LA was suppressed significantly. More interestingly, this kind of catalysts can promote the random copolymerization of ɛ -CL and L -LA. The transesterification side reaction and the polymer composition could be adjusted by modulating the electronic and steric effects of the ligand. In paticular, compound 3c could produce quasi-random copolymers without transesterification side reactions, as indicated by both the values of the reactivity ratios of the two monomers ( r LA = 1.31; r CL = 0.99) and the similar average lengths of the caproyl and lactidyl sequences ( L CL = 2.34; L LA = 2.44). Finally, a drug-random copolymer conjugates could be easily prepared by using 3c , indicating a potential application of 3c in pharmacutical and biomedical field. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2018 , 56 , 203–212
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RSC Advances
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2017
·Vol. 7
·期 4
·1947-1955
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IF 3.108
Xin Ran,Long Yang,Qing Qu,Shunling Li,Ying Chen,Limei Zuo,Lei Li
In this work, ultrasmall Pd–Pt bimetallic nanoclusters with a uniform size of 2.0 nm monodispersed on β-cyclodextrin functionalized reduced graphene oxide (β-CD-RGO) were successfully prepared in aqueous solution at room temperature within 30 min without the need for any organic solvent or high temperature. The integration of β-CD and RGO was responsible for the formation of the monodispersed 2.0 nm Pd–Pt bimetallic nanoclusters. Inspired by the monodisperse, ultrasmall, and pristine properties of the Pd–Pt clusters, the Pd–Pt@β-CD-RGO nanohybrid displayed enhanced activity for methanol and ethanol oxidation in alkaline media in comparison with the commercial Pd/C catalysts. This facile and simple method is of significance for the preparation of bimetallic nanocatalysts with high catalytic activity on suitable supporting materials.
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FOOD CHEMISTRY
·
2024
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IF 8.5
Jingjie Hou,Guixin Tan,Afeng Wei,Shan Gao,Huajiang Zhang,Wentao Zhang,Yujia Liu,Rui Zhao,Yunze Ma
In this study, a carboxymethylcellulose (CMC) induced depletion attraction was developed to stabilize high internal phase Pickering emulsions (HIPPEs) as age-friendly 3D printing inks. The results demonstrated that depletion force induced the adsorption of yolk particles at the droplet interface and the formation of osmotic droplet clusters, thereby increasing the stability of HIPPEs. In addition, the rheological properties and nutrient delivery properties of HIPPEs could be adjusted by the mass ratio of yolk/CMC. The HIPPEs stabilized at yolk/CMC mass ratio 20:7.5 showed optimal printability, viscoelastic, structural recovery, and swallowability. HIPPEs have been applied to 3D printing, International Dysphagia Dietary Standardization Initiative (IDDSI) test, and in vitro digestive simulation in the elderly, indicating their attractive appearance, safe swallowability, and enhanced bioaccessibility of β-carotene. Our work provides new ideas for developing age-friendly foods with plasticity and nutrient delivery capacity by depletion attraction stabilizing HIPPEs.
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FOOD HYDROCOLLOIDS
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2024
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IF 11
Jiarui Guo,Hongwei Zheng,Zihao Wei,Changhu Xue
Herein, a novel polysaccharide-polysaccharide colloidal complexes was prepared through self-assembly driven by electrostatic interactions between carboxymethyl-β-cyclodextrin (CM-β-CD) and chitosan (CS). The formation process, physicochemical properties, and emulsifying capacity of the CM-β-CD/CS complexes in varying mass ratios and pH values were systematically investigated, and the stabilization mechanism of CM-β-CD/CS complexes for Pickering emulsion was discussed. The findings demonstrated that the complex prepared at mass ratio 1:1 and pH 5 exhibited excellent interfacial activity and was able to absorb at interfaces to form interfacial films. Furthermore, it was capable of forming a reticular structure, which served as a synergistic stabilizer of the emulsions. Subsequently, this complex was selected to prepare Pickering emulsions loaded with Antarctic krill oil (AKO). The stability, microstructures, and rheological behavior of Pickering emulsion with varying particle concentrations and oil fractions were investigated. The results indicated that the AKO emulsions prepared from the oil fraction of 0.20 and 0.1% concentration of the complexes had the most robust physical stability. Besides, the AKO emulsion had enhanced oxidative stability and higher bioaccessibility than AKO. This study supports the development of polysaccharide-based emulsifiers and expands the application of AKO in the food industry.
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INDUSTRIAL CROPS AND PRODUCTS
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2024
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IF 5.6
Ling Sun,Lan Ding,Xiaoke Zhao,Doudou Yang,Xiaohui Liu,Yuanlin Ren,Yuesheng Li
Natural cellulose fabrics, as one of the combustibles prone to causing indoor fires, are modified with flame retardants encouraged by the fire protection industry. Herein, a bio-based colloid named ASDPC was prepared by 3-aminopropyltriethoxysilane, diethylenetriaminepentakis(methylphosphonic acid) and β-cyclodextrin, and used to enhance the flame retardancy of flax fabrics via the pad-dry-cure method. The treated flax fabrics named ASDPC-FF showed good flame retardancy with a damaged length of only 6.4 cm in the vertical burning test and a limiting oxygen index value of up to 33.8 %. Meanwhile, ASDPC-FF exhibited thermal stability and combustion behavior distinct from untreated flax fabrics. Thermogravimetric analysis presented that the char residue produced by ASDPC-FF at 800 °C was as high as 36.2 %. Cone calorimetry test indicated that the peak heat release rate and total heat release of ASDPC-FF decreased by approximately 89.0 % and 35.7 %, respectively. Furthermore, compared to untreated flax fabrics, condensed-phase and gas-phase studies found that ASDPC-FF turned into expanded char and produced fewer gaseous volatiles containing C-H, C O and C-O-C at high temperatures. More importantly, ASDPC-FF remained self-extinguishing after 25 laundering cycles with flame retardancy surpassing untreated flax fabrics. In summary, this work provides a novel route for expanding the manufacture of flame-retardant flax fabrics.
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Food Bioscience
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2024
·
IF 4.8
Yao Wang,Peng Zhang,Haowen Lin,Xiaoyun Fei,Guowen Zhang,Xing Hu
The aim of this study was to investigate the effect of rosmarinic acid (RA) on the structure and functional characteristics of β -Lactoglobulin ( β -LG) under two different covalent conditions (alkaline and free radical grafting). The successful covalent grafting of RA was predicted by the increase in the molecular weight of the complex, the total phenolic content, and the change in the reactive groups. The structure of the alkaline-modified conjugates gradually tightened with increasing RA concentration, whereas the structure of the free radical-grafted conjugates gradually stretched. Overall, the structure of β -LG became more stable (the number of α-helix and β-sheet rose), effectively enhancing the thermal stability of β -LG. In addition, the introduction of RA improved the interfacial wettability, emulsification, and antioxidant capacity of β -LG. This study provided some theoretical insights into the interactions between proteins and polyphenols.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Guangliang Xing,Jia Liu,Ruyi Tang,Tianran Hui
BACKGROUND The escalating global prevalence of food allergies has intensified the need for hypoallergenic food products. Transglutaminase (TGase)-mediated crosslinking has garnered significant attention for its potential to reduce the allergenicity of food proteins. This study aimed to investigate the effects of TGase crosslinking on the potential allergenicity and conformational changes in a dual-protein system composed of β -lactoglobulin ( β -LG) and soy protein isolate (SPI) at varying mass ratios (10:0, 7:3, 5:5, 3:7 and 0:10 (w/w)). RESULTS TGase preferentially crosslinked the 7S and 11S subunits of soy protein, rather than β -LG. Crosslinking treatment reduced the allergenic potential of both soy protein and β -LG, with the degree of reduction depending on the protein ratio. The β -LG5:SPI5 and β -LG7:SPI3 mixtures showed the most significant reduction in antibody reactivity towards soy protein and β -LG, respectively. Additionally, TGase-mediated crosslinking significantly reduced the binding capacity of all dual-protein samples to serum immunoglobulin E from allergic patients, compared to the control group ( P < 0.05). The allergenicity reduction was accompanied by structural modifications, including a decrease in β -sheet content, an increase in β -turn and random coil structures, enhanced ultraviolet absorption and intrinsic fluorescence, reduced free sulfhydryl levels and altered intermolecular forces. These changes suggest that TGase-induced crosslinking may disrupt or mask allergenic epitopes, thus lowering allergenicity. CONCLUSION TGase crosslinking effectively reduced the allergenic potential of the dual-protein system by inducing structural changes. These findings underscore the potential of TGase in developing hypoallergenic dual-protein food products and provide a scientific foundation for future research and application in the food industry. © 2025 Society of Chemical Industry.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Hang Yuan,Samaila Boyi Ajeje,Yunzhe Wen,Chonlong Chio,Yun Hu,Shaohua Dou,Wensheng Qin,Ezhen Zhang,Qun Wu,Fubao Sun
Endo-1,4-β-glucanase plays a crucial role in converting cellulose from lignocellulosic biomass into fermentable sugars for biofuel production. However, its commercial utility is hindered by poor catalytic performance under extreme conditions. This study enhanced the catalytic activity of the endo-1,4-β-glucanase ( Tn CelB) from Thermotoga neapolitana through error-prone PCR directed evolution. After screening >4000 colonies, three mutants with enhanced activity were obtained. Mutants Tn CelB Y88F , Tn CelB A233T , and Tn CelB W219R displayed 52.14 U/mg, 44.90 U/mg, and 34.70 U/mg of specific activity on CMC, respectively, which is 1.9, 1.7, and 1.3 times higher than that of the wild-type (26.74 U/mg), correspondingly. Likewise, their enzyme activity on barley β-D-glucan increased by 3.5, 2.2, and 1.8 times, respectively. Notably, Tn CelB Y88F maintained over 90 % activity after 60 mins at high temperatures (80–100 °C), indicating an exceptional thermostability. Protein docking revealed that Tn CelB Y88F had higher binding affinity, aligned with kinetic studies. Tn CelB was capble of released more non-oxidized sugars from the hydrolysis of regenerated amorphous cellulose (RAC) by synergy with auxiliary action family 10 ( AA 10), which is potential in development of efficient lignocellulosic saccharification. This study can provide useful insights for the future engineering of other endoglucanases in the glycoside hydrolases family 12.
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