JOURNAL OF CHEMICAL THERMODYNAMICS
·
2017
·
IF 2.726
Hui Ding,Wenjing Ke,Dan Zhao,Lulu Wang,Yujie Gao,Shejiang Liu
In this work, we measured the isobaric vapor-liquid equilibrium (VLE) data for the binary system of methyl caprate (1) + methyl laurate (2) at 2,4 and 6 kPa with a modified Othmer still. The point consistency test of Van Ness test method was applied to check the thermodynamic consistency of all the experimental values, which guaranteed the reliability of our measurements. Afterwards, the experimental data were regressed by two activity coefficient models (Wilson, NRTL). Furthermore, we made the data prediction with the group contribution method (UNIFAC) and the COSMO-SAC model. The results of all the regressions and predictions are satisfying and reasonable, while the results provided by the NRTL model are relatively better than the other three models, with the root mean square deviations of temperature and vapor-phase mole fraction less than 0.33 K and 0.0069, respectively.
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高影响因子
RSC Advances
·
2016
·Vol. 6
·期 107
·105723-105728
·
IF 3.289
Cheng Chen,Yan Li,Lijian Liu
Oligomers as fluorescent macromolecules are of significant importance and are fairly attractive for high quantum yield two-way conversion luminescence. We report a high conversion rate (ΦF = 0.5184) for a fluorescent oligomer, 1,4-butanediol-bis(5-carbonyl-3-carbethoxy-2-pyrazoline). It was prepared by the novel cyclopolycondensation of 1,4-butanediol-bis(5-carbonyl-3-carbethoxy-2-pyrazoline), a chromophore with good fluorescence (ΦF = 0.0573). After polymerization, a green fluorescent protein (GFP)-mimetic structure was formed, in which a methylene chain (–CH2–CH2–CH2–CH2–) as a flexible group is beneficial due to its folding, and an azo group sits in the rigid pyrazoline ring because of a resonance effect. These factors collectively contribute to the excellent efficiency of the fluorescence performance of the oligomer. Additionally, the oligomer exhibited a weak red shift and a significant fluorescence enhancement compared with the monomer, and we achieved good quality live-cell imaging. The oligomer also allowed for the enhancement of fluorescence intensity through the two-wavelength excitation of a cell rather than increasing the dosage of the dye or the power of the laser.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2024
·
IF 4.2
Maoshan Wang,Yingxue Liu,Houda Gui,Gaoqiang Ma,Binyang Li,Zhanwei Zhang,Gyeonghwi Yu,Ailin Wu,Xin Xu,Dongjiao Zhang
Eldecalcitol (ED-71), a novel active form of vitamin D, shows potential in treating osteoporosis. However, its underlying mechanisms of action remain to be determined. This study aimed to investigate the effect of ED-71 on bone regeneration and to illustrate its mode of action. The in-vitro model was developed using rat primary osteoblasts cultured under high-glucose conditions, and these cells were treated with ED-71. Additionally, an in vivo model of cranial bone defects was established in type 2 diabetic rats, and ED-71 was administered by gavage. The results demonstrated that ED-71 prevented osteoblast cell death, enhanced rat primary osteoblasts' osteogenic capacity, and attenuated the overexpression of hypoxia-inducible factor 1α (HIF1α) induced by high glucose levels. Furthermore, ED-71 increased glutathione peroxidase 4 (GPX4) levels and inhibited ferroptosis in response to hyperglycemic stimulation. Notably, interference with the HIF1α activator and ferroptosis activator Erastin significantly reduced the therapeutic effects of edetate osteolysis. These findings were further tested in vivo experiments. These results suggest that ED-71 activates the HIF1α pathway in vivo and in vitro, effectively relieving the ferroptosis induced by high glucose. Significantly, ED-71 may improve osteogenic disorders caused by diabetes.
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PubMed
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CytoJournal
·
2024
·
IF 2.5
Yan Li,Zhenwei Jia,Xiaoyang Kong,Hongbo Zhao,Xiaoyan Liu,Guirong Cui,Jianmin Luo
Objective:
We currently face a sharp increase of T-cell acute lymphoblastic leukemia (T-ALL) incidence and a challenge of unmasking its complex etiology. The deoxycytidine analog 5-Aza-2’-deoxycytidine (5-Aza-dC) is currently the most common nucleoside methyltransferase inhibitor. The objective of this study was to clarify the role of 5-Aza-dC in T-ALL cell biological behaviors and phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression.
Material and Methods:
T-ALL cell lines were divided into the experimental group with 5-Aza-dC solution treatment, and the control group without treatment. PTEN methylation was detected using methylation-specific polymerase chain reaction (MS-PCR). Following the measurement of cell proliferation, viability, apoptosis, invasion, migration, etc., quantitative reverse transcription-polymerase chain reaction (PCR) was conducted to detect PTEN, DNA methyl-transferases (DNMT1), DNMT3a, MBD2, and MeCP2 expressions; Western blot to detect PTEN, PI3K, AKT, and mTOR protein expressions. In addition, rescue experiments to inhibit and restore the expression of PTEN in different groups were performed for further identification of the results in the former parts.
Results:
MS-PCR results showed that in Jurkat cells, the target band was amplified using methylated primers for the PTEN gene promoter region; moreover, at 10 μmol/L of 5-Aza-dC for 24 h, PTEN methylation was completely removed without any un-methylated band observed. The experimental group had significantly lower cell proliferation and viability rates, higher apoptosis rates, decreased cell proportion in S phase, reduced invasion and migration; increased PTEN expression, decreased DNMT1, DNMT3a, MBD2, and MeCP2 mRNA expressions; and decreased PI3K, AKT, and mTOR protein expressions than those in the control group (all P < 0.05). Furthermore, according to the rescue experiment, silenced PTEN expression weakened the beneficial roles of 5-Aza-dC treatment, and resulted in significantly higher cell proliferation and viability rates, lower apoptosis rates, increased cell proportion in S phase, increased cell invasion and migration; decreased PTEN expression, elevated DNMT1, DNMT3a, MBD2, and MeCP2 mRNA expressions, and higher PI3K, AKT, and mTOR protein expressions (all P < 0.05). While restored PTEN expression enhanced functions of 5-Aza-dC treatment, leading to obviously lower cell proliferation and viability rates, higher apoptosis rates, increased cell proportion in G1 phase, and reduced cell invasion and migration; as well as increased PTEN expression, decreased DNMT1, DNMT3a, MBD2, and MeCP2 mRNA expressions, and lower PI3K, AKT, and mTOR protein expressions (all P < 0.05).
Conclusion:
Demethylation treatment with 5-Aza-dC can inhibit T-ALL cell malignant biological behaviors and enhance the sensitivity to chemotherapy agents possibly, which may be related to the inhibited expressions of DNMT1, DNMT3a, MBD2, and MeCP2, and restored expression activity of PTEN to negatively regulate the PI3K/AKT signal transduction. Our silencing and restoration of PTEN expressions further support our findings, highlighting that demethylation with 5-Aza-dC to restore the anti-tumor activity of the tumor suppressor gene PTEN may be a promising therapeutic option for treating T-ALL.
Keywords: 5-Aza-2’-deoxycytidine, T-cell acute lymphoblastic leukemia, PTEN, Tumor cell biological behaviors, Demethylation
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2024
·
IF 6.9
Linquan Dai,Bo Feng,Mengyan Tang,Yuangan Chen
It is challenging to separate between molybdenite and chalcopyrite due to their comparable hydrophobic characteristics. Inorganic depressants were previously used to separate two minerals, but the results were unsatisfactory and polluted the environment. In this study, the molybdenite and chalcopyrite were separated using a highly effective and ecologically safe depressant, 2,4-Diamino-6-hydroxypyrimidine (DAHP). According to the single mineral experiment, chalcopyrite is totally depressed while molybdenite has superior floatability. The efficacy of DAHP as the depressant was validated by further artificially mixed mineral flotation experiments, indicating the possibility of separating chalcopyrite and molybdenite. The adsorption capacity experiment results showed that the adsorption capacity of DAHP on the surface of chalcopyrite was greater than that on the surface of molybdenite. Zeta potential experiment results show that DAHP is adsorbed on the surface of chalcopyrite and molybdenite through static electrostatic interactions . X-ray photoelectron spectroscopy (XPS) experiment results show that DAHP is mainly adsorbed on chalcopyrite surface through –NH 3 + group, which hinders the adsorption of kerosene. The electrostatic contact between DAHP and molybdenite has the least effect on kerosene adsorption. This study shows that DAHP has the potential to separate chalcopyrite and molybdenite as an efficient and environmentally friendly depressant.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Shulin Liu,Muhammad Junaid,Chong Wang,Jun Wang
Nanoplastics (NPs, < 1000 nm) interact with chemicals and biomolecules to produce chemical−/eco-corona, altering the environmental destiny, bioavailability, and toxicity of plastic particles and co-occurring chemicals. This study employs exogenous (humic acid, HA) and endogenous (bovine serum albumin, BSA) natural organic matter (NOM) to investigate the eco-corona formation on NPs and explore the interfacial effects of eco-corona and 6:2 chlorinated polyfluorinated ether sulfonate (Cl-PFESA, commonly named as F-53B) on zebrafish ( Danio rerio ) after 7 days of exposure. Our results indicated significant changes in growth and developmental indices of zebrafish embryos among all eco-corona groups ( p < 0.05). Additionally, NFB (BSA-corona, 1 mg/L NPs + 200 μg/L F-53B + 10 mg/L BSA), NFH (HA-corona, 1 mg/L NPs + 200 μg/L F-53B + 10 mg/L HA) and NFHB (BSA-HA-corona, 1 mg/L NPs + 200 μg/L F-53B + 10 mg/L BSA + 10 mg/L HA) showed elevated bioaccumulation of NPs, ROS generation and induction of apoptosis. Transcriptomic analysis showed the number of differentially expressed genes (DEGs) in the following order: BSA-HA-corona (NFHB (2953) > HA-corona (NFH (2797) > NH (2721) > F-53B (2292) > NF (2033) > BSA-corona (NFB (687) > NB (450)), and no DEGs were detected in the single NP compared to the control. Further, the PI3K-AKT, immune system, endocrine system, digestive system, infectious diseases, and neurovegetative disease pathways showed sensitive responses in the NFH/NFHB groups compared to those in the NFB group. Therefore, the interactive effects of NPs and F-53B on zebrafish embryos were lower in the presence of BSA-corona than those in HA- or HA-BSA-coronas, indicating a relationship between the formation of diverse eco-coronas on NPs by multiple NOM and an associated increase in the interfacial toxicological effects of plastic particles and F-35B in freshwater organisms.
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PubMed
高影响因子
APPLIED THERMAL ENGINEERING
·
2024
·
IF 6.1
Lisheng Deng,Shusen lin,Linfa Xiao,Mitsuhiro Kubota,Hongyu Huang,Jun Li,Dezhen Chen
The application of MgO/Mg(OH) 2 in heat storage technology has been limited due to slow hydration rate and easy agglomeration. In this paper, nanoporous carbon particle (NCP) as a carrier was used to prepare NCP/MgO chemical heat storage composite materials by impregnation and calcination methods, respectively. The microstructure characterization, hydration reaction, and simultaneous thermogravimetric analysis experiments were carried out on the prepared composite materials. The results showed that the internal distribution of MgO in the composite material prepared by calcination method was more uniform, and the particle size of MgO was smaller. Under the conditions of 110 ℃ hydration temperature and 57.8 kPa water vapor partial pressure, the hydration conversion ratio of the composite material prepared by calcination method after 120 min of hydration conversion ratio reached 93 %, which was 17.7 % higher than that of the impregnated composite material and 50 % higher than that of pure MgO. Under the comprehensive influence of specific surface area, pore structure, and active component particle size, compared with pure materials and impregnated composite materials, the dehydration rate of the composite material prepared by calcination method at 300 ℃ was twice that of impregnated method and 6 times that of pure MgO, and the heat storage density reached 1039kJ/kg. After 10 cycles, the heat storage density still maintained an initial state of 95.8 %. Compared with pure materials and impregnated composite materials, NCP/MgO composite materials prepared by calcination method had better heat storage performance and higher cyclic stability.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2024
·
IF 2
Yong Zheng,Jiahuan Luo,Ling Li,Qian Wang,Huichao Lv,Kai Wang,Changwei Lai
In this study, six pure 1-alkyl-2-methylpyrazolium-based ionic liquids (ILs) with methyl sulfate and bis(trifluoromethylsulfonyl)imide anions ([Cnmpz][MeSO4] and [Cnmpz][NTf2], n = 2, 3, and 4) were prepared and characterized. The density, viscosity, and refractive index of these pure ILs were measured from 298.15 to 353.15 K at 101.3 kPa, which fitted well with the corresponding empirical equations. Moreover, the values of the isobaric thermal expansion coefficient were also obtained. The results of experiments and density functional theory calculations indicate that the adjacent positions of two nitrogen atoms in pyrazolium cations lead to the close packing of ions, strong cation–anion interaction, and high molar polarizability per unit volume. The effects of temperature and N-alkyl chain length on the density, viscosity, and refractive index of these ILs are similar to those for imidazolium-based salts. Compared with [Cnmpz][MeSO4], [Cnmpz][NTf2] exhibits a higher density, lower viscosity, and lower refractive index under the same conditions. Moreover, the correlation between density and refractive index was studied, and a new empirical equation was proposed to express this correlation at different temperatures. It is expected that this work will enable deeper insights into the properties, structure, and structure–property relationship of pure pyrazolium-based ILs.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Youwei Cheng,Lian Zhu,Yaqiong Gong
Metal-organic frameworks (MOFs) are widely recognized as precursors or sacrificial templates for the synthesis of target catalysts on account of their high porosity and high active surface area . Elemental doping strategy is considered as an effective method for the synthesis of efficient and stable catalysts for electrolytic water. Therefore, in this paper, Ce-doped Ni(OH) 2 nanoparticles and Ni-MOF nanosheets composites were synthesized by electrodeposition using Ni-MOF as precursor. Catalyst Ce–Ni(OH) 2 @Ni-MOF/NF displays excellent OER performance, requiring only 193 mV and 279 mV overpotentials to realize current densities of 10 mA cm −2 and 100 mA cm −2 , respectively, while the UOR performance was very remarkable, only a potential of 1.28 V was demanded to reach a current density of 10 mA cm −2 . In addition, no obvious change in the overpotential was noticed during the stability test of 16 h, indicating good stability of Ce–Ni(OH) 2 @Ni-MOF/NF. This study provides a meaningful reference for the design of an efficient and stable environmental friendly electrocatalyst .
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Yujie Sha,Duo Zhang,Jiazichao Tu,Ruyue Zhang,Yijia Shao,Jimei Chen,Shaoyou Lu,Xiang Liu
Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is a widely used organophosphate ester that can adversely affect animal or human health. The intestinal microbiota is critical to human health. High-dose exposure to TDCIPP can markedly affect the intestinal ecosystem of mice, but the effects of long-term exposure to lower concentrations of TDCIPP on the intestinal flora and body metabolism remain unclear. In this study, TDCIPP was administered to Sprague–Dawley rats by gavage at a dose of 13.3 mg/kg bw/day for 90 days. TDCIPP increased the relative weight of the kidneys ( P = 0.017), but had no effect on the relative weight of the heart, liver, spleen, lungs, testes, and ovaries ( P > 0.05). 16 S rRNA gene sequencing revealed that long-term TDCIPP exposure affected the diversity, relative abundance, and functions of rat gut microbes. The serum metabolomics of the rats showed that TDCIPP can disrupt the serum metabolic profiles, result in the up-regulation of 26 metabolites and down-regulation of 3 metabolites, and affect multiple metabolic pathways in rat sera. In addition, the disturbed genera and metabolites were correlated. The functions of some disturbed gut microbes were consistent with the affected metabolic pathways in the sera, and these metabolic pathways were all associated with kidney disease, suggesting that TDCIPP may cause kidney injury in rats by affecting the intestinal flora and serum metabolism.
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高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 44
·8770-8779
·
IF 4.543
Jiachen Sun,Yanxia Zhang,Bin Li,Yong Gu,Liang Chen
Stimulating the adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is crucial for the scaffold materials used in bone tissue engineering. In this study, a silk fibroin (SF)/nano-hydroxyapatite (nHAp) scaffold was prepared, embedded with SF microspheres containing bone morphogenetic protein-2 (BMP-2) and surface-modified through chemical conjugation with the type I collagen-mimetic peptide GFOGER. Surface-bound GFOGER and sustained BMP-2 release promoted the adhesion and osteogenic differentiation, respectively, of BMSCs. Our results indicated that this system released low doses of BMP-2 in a sustained manner, thereby avoiding cost and safety problems. The biological responses to this scaffold were investigated both in vitro and in vivo. Our results indicated that the synergistic effect of GFOGER and BMP-2 promoted the adhesion and osteogenic differentiation of BMSCs, thus leading to complete bone bridging of rat cranial defects after 12 weeks of implantation. This system might provide a powerful platform for treating bone defects and for bone tissue engineering.
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高影响因子
CHIRALITY
·
2017
·Vol. 29
·期 7
·340-347
·
IF 1.956
Hongxing Dong,Danxia Zhang,Hailong Lin,Yudan Wang,Lijia Liu,Meixia Zheng,Xiaobo Li,Chunhong Zhang,Junqing Li,Peng Zhang,Juhyok So
Acrylamide (AM) was copolymerized with ethylene glycol dimethacrylate (EGDMA) in the presence of ( R )-1,1′-binaphthalene-2-naphthol (BINOL) as the template molecules on the surface of silica gel by a free radical polymerization to produce a chiral stationary phase based on the surface molecularly imprinted polymer (SMIP-CSP). The SMIP-CSP showed a much better separation factor ( α = 4.28) than the CSP based on the molecularly imprinted polymer (MIP-CSP) without coating on the silica gel ( α = 1.96) during the chiral separation of BINOL enantiomers by high-performance liquid chromatography. The influence of the pretreatment temperature and the content of the template molecule (( R )-BINOL) of the SMIP-CSP, and the mobile phase composition on the separation of the racemic BINOL were systematically investigated.
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