MICROCHIMICA ACTA
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2026
·Vol. 193
·期 3
·146
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IF 5.4
Li Ruiwen,Shen Hao,Shi Dandan,Yu Shaoning,Qian Guoqing
An aptamer-gated metal-organic framework-based biosensing platform for highly sensitive detection of multidrug-resistant bacteria (MDRB) has been developed. This biosensing platform utilizes aptamers as specific “gated” switches, which are integrated with the UiO-66-NH₂ MOFs to construct a “gated” sensing system loaded with 3,3′,5,5′-tetramethylbenzidine (TMB). The entry of target bacteria induces specific dissociation of sDNA/Apt, forming an aptamer-bacteria complex on the UiO-66-NH₂ surface while releasing TMB into the solution. Subsequently, the PCN-224-Mn nanozyme catalyzes the oxidation of TMB, generating a colorimetric signal. This biosensing platform demonstrates a broad linear range for detecting New Delhi metallo-β-lactamase-1 Klebsiella pneumoniae (NDM-1 KP) and multidrug-resistant Acinetobacter baumannii (MDR-AB), achieving ultra-high sensitivity with detection limits of 5 CFU/mL and 7 CFU/mL, respectively. The practical performance of this biosensing platform was evaluated through spiked recovery experiments. Its excellent recovery demonstrates that the method maintains high reliability and stability even when applied to complex real-world samples, providing an effective technical solution for addressing challenges in microbial detection in practical scenarios. Graphical
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Nature Communications
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2026
·Vol. 17
·期 1
·1663
·
IF 18.1
Zhang Yan,Liu Zhiqiang,Kong Wenyu,Ao Yanxiao,Zhang Rui,Du Yanan
CAR-macrophage (CAR-M) therapy holds promise for the treatment of tumor and fibrotic diseases, yet genetic engineering remains the main efficacy-enhancing approach. Here we develop a non-genetic strategy using viscoelastic hydrogel to mechanically prime CAR-Ms. CAR-Ms exhibit potent phagocytosis of activated fibroblasts and collagen-degradation capacity, and hydrogel priming further enhances their cytotoxicity. Mechanistically, viscoelastic hydrogel priming reduces CAR-M membrane tension, which triggers membrane CAR to disassemble from clusters into dispersed monomers and dimers, leading to enhanced downstream signaling. In pulmonary fibrosis models, hydrogel-primed CAR-Ms demonstrate superior therapeutic outcomes, showing fibrosis reduction and microenvironment improvement. This study highlights CAR-M therapy’s potential for pulmonary fibrosis treatment and offers a distinct approach to improve CAR-M efficacy by physical stimuli.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 3
·134
·
IF 5.4
Wu Jiayi,Li Chengwei,Zhao Jingwen,Liu Jiyang
A Mn-doped Fe 3 O 4 (Mn-Fe 3 O 4 ) nanozyme was synthesized using a one-step solvothermal method. Compared to pristine Fe 3 O 4 , Mn-Fe 3 O 4 exhibits unique oxidase (OXD)-like activity and enhanced peroxidase (POD)-like activity. The superior catalytic performance arises from the incorporated Mn species and the associated oxygen vacancies facilitate oxygen adsorption and activation, establishing an efficient self-cascade catalytic system. In this system, hydrogen peroxide (H 2 O 2 ) generated in-situ through OXD-like catalysis can trigger mimic-POD reactions. A dual-mode antioxidant detection platform was subsequently constructed using melatonin (MT) as a proof of concept, with 3,3’,5,5’-tetramethylbenzidine (TMB) serving as the substrate for the Mn-Fe 3 O 4 nanozyme. The colorimetric mode enables rapid visual screening by monitoring the oxidation of TMB to a blue-colored product, achieving a detection limit of 0.13 µM. The electrochemical mode features the novel utilization of mesoporous silica nanochannels for the selective enrichment of oxidized TMB (oxTMB), enabling highly sensitive quantification of MT with a detection limit as low as 0.6 nM. The assay applied to commercial tablets shows excellent recovery and reproducibility. This work combines engineered nanozymes with nanofilm-modified electrodes, offering an innovative approach for constructing advanced multimodal sensing systems. Graphical
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Nature Communications
·
2026
·
IF 18.1
Guo Jiaxin,Wang Ruguang,Yang Yuting,Zhang Qinhao,Cao Fahe,Zhao Jiong,Pan Caofeng,Ling Tao
Anion exchange membrane water electrolyser is a highly promising electrolyser technology, but its performance in pure water is severely limited by the unsatisfactory OH − conductivity of the membrane. To overcome this critical challenge, we develop a local alkalinity engineering strategy that employs TiO 2 nanoparticles in catalyst layers. These nanoparticles enrich OH − in the electric double layer at both electrodes, creating self-sustaining alkaline microenvironments (pH ~ 14), as confirmed by a scanning electrochemical microscopy technique integrating pH microelectrodes. As a result, the engineered electrolyser achieves a high current density of 3.0 A cm −2 at 2.08 V, approaching that of the precious-metal-based proton exchange membrane water electrolyser under identical conditions. In addition, the local alkalinity alleviates the degradation of non-noble metal catalysts and membrane, thus enabling the electrolyser to realise long-term stability of ~ 1400 h at 1.0 A cm −2 . We also demonstrate that this local alkalinity strategy can be readily extended to different types of membranes and scaled up, providing a universal tactic to boost the performance of anion exchange membrane water electrolysers.
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Nature Communications
·
2026
·
IF 18.1
Peng Jiaxing,Wu Taizheng,Zeng Liang,Liu Zhou,Li Shiqi,Feng Guang
Amorphous porous carbons have been widely used as electrodes for energy storage. However, due to their structural heterogeneity and complex pore topology, the absence of reliable atomic carbon models hinders understanding energy storage mechanisms through molecular simulations. Here, we develop a modeling approach capturing the rich experimental information of small angle X-ray scattering, gas adsorption, and material density, integrating three-dimensional morphology construction with atomic structure generation. Using the built atomic model, constant-potential molecular simulations are performed to investigate the capacitive behavior of amorphous porous carbons, well-validated by experimental measurements of capacitance and impedance. Voronoi sphere analyses uncover the interplay between pore structure and charge storage: ultramicropores (< 0.7 nm) are found to enhance the capacitance through ion exchange, while large micropores (> 0.7 nm) exhibit lower capacitance with negligible ion number change. The charging dynamics are quantified by the proposed multi-scale impedance model, bridging microscopic simulations and macroscopic experiments. This modeling framework paves the way toward molecular understanding of energy storage in amorphous porous materials.
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Cell Death & Disease
·
2026
·
IF 12.2
Yang Dian,Liu Yichao,Hong Yueshun,Miao Enming,Wang Peng,Sun Yuming,Zhou Lina,Liu Shuyan,Zhang Yingqiu,Qin Hongqiang,Ye Mingliang,Liu Han
Stress granules (SGs) are membrane-less organelles formed through liquid-liquid phase separation of proteins and RNAs, serving as temporary repositories for biomacromolecules to protect cells under stress conditions. Impaired SG disassembly is closely implicated in neurodegenerative diseases and aging, yet the mechanisms regulating SG dynamics are incompletely investigated. The constituents of heterogenous SGs are complicated and broadly categorized as core and shell components. In contrary to the relatively stable core components, our understanding of the diverse SG shell is deficient. By combining interactomic and proximity proteomic approaches, we reveal that the deubiquitinating enzyme OTUD6B is associated with SG-related functions. Immunofluorescence assays showed that OTUD6B localized to SGs, as well as regulated their early assembly and clearance, partially dependent on its enzymatic activity. Further proximity proteomics and interactomics results uncover the ATPase VCP/p97, a key SG disassembly factor, as an OTUD6B-associated protein. OTUD6B and VCP association is governed through their disordered regions normally participated in biomolecular condensation. VCP knockdown or pharmacological inhibition phenocopied OTUD6B silencing by leading to defects in SG dynamics. Mechanistically, SG coalescence of VCP incurred by OTUD6B in a partially enzymatic activity-dependent manner functions to accelerate not only the early assembly, but also SG clearance following stress removal. Therefore, our findings establish OTUD6B as a critical modulator of SG dynamics, linking its function to stress responses and potential disease mechanisms.
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Nature Energy
·
2026
·
IF 70.1
Wang Deng,Liu Zhixin,Gao Zhi-Wen,Lei Xia,Zhu Peide,Zeng Jie,Li Qian,Wang Lida,Zhang Zhen,Gu Meng,He Siru,Bao Yuqi,Lian Qing,Li Jingbai,Song Zonglong,Xu Yintai,Lei Dangyuan,Wang Xingzhu,Jen Alex K.-Y.,Xu Baomin
Hole-selective self-assembled monolayers have advanced the performance of perovskite solar cells (PSCs), yet their excessive intermolecular interactions result in undesirable self-aggregation and weak interfacial contact. Here we devise a bicarbazole-based dimeric structure incorporating amide units as dual hydrogen-bond donors and acceptors to enable the formation of hydrogen-bonding networks within the molecules and with transparent conductive oxide. This design promotes homogeneous molecular arrangement and well-aligned energy levels, minimizing hole-transport loss and enhancing interfacial stability. We achieve an efficiency of 21.56% in a 1.77 eV PSC, with an open-circuit voltage of 1.35 V and a fill factor of 85.76%. This strategy is also applicable to 1.56 eV PSCs, affording efficiencies of 26.80% (certified 26.57% by current density–voltage ( J – V ) scan and 25.92% steady-state measured over 300 s). Most notably, the integrated all-perovskite tandem solar cell yields an efficiency of 30.19% (certified 29.38% by J – V scan and 28.40% steady state measured over 120 s).
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IONICS
·
2020
·Vol. 26
·期 8
·3823-3830
·
IF 2.394
Zhang Jun-Hong,Yue Haoguo,Ma Yueqiang,Han Xiao,Mei Jie,Zhao Jinsheng,Li Yan-Mei,Zhu Yan-Rong
In this work, the spherical NiO-NiMoO 4 composite was synthesized by solvothermal route followed by calcination, and then spherical NiO-NiMoO 4 /PPy composite was synthesized by in situ polymerization of pyrrole monomer. The NiO-NiMoO 4 and NiO-NiMoO 4 /PPy microspheres with a particle size of around 1–2 μm consist of nanorods and nanosheets. Hence, NiO-NiMoO 4 /PPy anode shows a higher delithiation and lithiation capacities than NiO-NiMoO 4 at each rate. At 0.2 A g −1 , NiO-NiMoO 4 only shows an initial delithiation capacity of ∼775 mAh g −1 , and the capacity only keeps 207 mAh g −1 after 160 cycles. Nonetheless, NiO/NiMoO 4 /PPy shows an initial delithiation capacity of ~ 884 mAh g −1 , and the capacity also maintains ~ 846 mAh g −1 even after 160 cycles. The corresponding capacity retention of NiO/NiMoO 4 /PPy and NiO/NiMoO 4 are 95.7% and 26.7%, respectively. The PPy modification is a direct and effective strategy to improve the reversible capacity and cycling performance of NiO-NiMoO 4 composite.
研究领域:
材料科学
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储能
·
电化学
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Science China-Materials
·
2021
·Vol. 64
·期 9
·2251-2260
·
IF 8.273
Liu Guiheng,Su Jianwei,Feng Xin,Li Huiqiao,Zhai Tianyou
Manganese phosphorous selenium (MnPSe 3 ), as a representative of layered metal phosphorus trichalcogenides (MPTs), has gained significant attention due to its direct bandgap, high carrier mobility, large absorption coefficient, which indicate great potential in photoelectric application. Herein, high-quality two-dimensional (2D) MnPSe 3 flakes were mechanically exfoliated from the corresponding bulk crystals synthesized by chemical vapor transport (CVT) methods. The systematic investigation was applied to the lattice vibrations of MnPSe 3 via angle-resolved polarized Raman spectroscopy (ARPRS), and the Raman vibration modes were determined based on Raman selection rules and crystal symmetry. Impressively, the photodetectors based on 2D MnPSe 3 flakes exhibit excellent photoresponse to the ultraviolet light with a responsivity up to 22.7 A W −1 and a detectivity of 2.4 × 10 11 Jones. The high performance in the ultraviolet range signifies that 2D MnPSe 3 is expected to be a powerful candidate for future ultraviolet photodetection.
研究领域:
材料科学
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纳米技术
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光电子学
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固态物理
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JOM
·
2017
·Vol. 69
·期 10
·1963-1969
·
IF 1.86
Xie Kaiyu,Shi Zhongning,Xu Junli,Hu Xianwei,Gao Bingliang,Wang Zhaowen
Aluminothermic reduction-electrolysis using an inert anode process is proposed to extract oxygen and metals from Minnesota Lunar Simulant-1 (MLS-1). Effective aluminothermic reduction between dissolved MLS-1 and dissolved metal aluminum was achieved in cryolite salt media. The product phases obtained by aluminothermic reduction at 980°C for 4 h were Al, Si, and Al 5 FeSi, while the chemical components were 79.71 mass% aluminum, 12.03 mass% silicon, 5.91 mass% iron, and 2.35 mass% titanium. The cryolite salt containing Al 2 O 3 was subsequently electrolyzed with Fe 0.58 -Ni 0.42 inert anode at 960°C for 4 h. Oxygen was evolved at the anode with an anodic current efficiency of 78.28%. The results demonstrate that this two-step process is remarkably feasible for the extraterrestrial extraction of oxygen and metals. This process will help expand the existing in situ resource utilization methods.
研究领域:
空间资源利用
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提取冶金
·
电化学
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月球探测
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EMBO Molecular Medicine
·
2026
·Vol. 18
·期 5
·1990
·
IF 7.9
Yini Huang,Yan Ye,Xinmei Zhu,Dengpan Liang,Ruiqin Cui,Xiaopeng Yuan,Xitao Li,Quanming Zou,Haibo Li,Wei Huang
The limitations of existing drugs and the development of drug resistance make it urgent to develop new drugs against methicillin-resistant Staphylococcus aureus (MRSA). The re-development of the antibacterial activity of drugs that have already been proven safe for human use is an effective way. In this study, we discovered that the Src homology region 2 domain-containing phosphatase-1 (SHP-1) agonist SC-43, exhibits potent activity against Gram-positive bacteria, including MRSA. The mode of action studies revealed that SC-43 inhibits the key enzyme coproporphyrin ferrochelatase (CpfC) of the coproporphyrin-dependent (CPD) heme synthesis pathway and interferes with the bacterial porphyrin metabolism. The determination of the structure of CpfC derived from S. aureus (SA CpfC ) in this study allowed us to reveal the inhibitory effect of SC-43 at the molecular level. Animal experiments showed that SC-43 has the potential to become a new anti-MRSA drug. In conclusion, this study discovered a new anti-MRSA activity of a drug currently undergoing clinical trials and simultaneously verified the feasibility of developing new anti-Gram-positive bacteria drugs by inhibiting the CPD pathway. The limitations of existing drugs and the emergence of drug resistance make the development of new therapies against methicillin-resistant Staphylococcus aureus (MRSA) increasingly urgent. Here, we demonstrate that the Src homology region 2 domain-containing phosphatase-1 (SHP-1) agonist SC-43 exhibits antibacterial activity against MRSA. The limitations of existing drugs and the emergence of drug resistance make the development of new therapies against methicillin-resistant Staphylococcus aureus (MRSA) increasingly urgent. Here, we demonstrate that the Src homology region 2 domain-containing phosphatase-1 (SHP-1) agonist SC-43 exhibits antibacterial activity against MRSA.
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Cancer Cell International
·
2026
·
IF 7
Ni Jie,Gao Aidi,Chen Ying,He Chao,Wang Xixi,Savelyeva Natalia,Dong Xin,Wang Yiqiang,Wang Mu
Background Despite significant advances in the treatment of clear cell renal cell carcinoma (ccRCC) over the past two decades, therapeutic intervention for patients at advanced or metastatic stages remains a clinical hurdle. Emerging evidence highlights aberrant CD70 expression during tumor progression, supporting its potential as a therapeutic target in advanced ccRCC. However, the molecular mechanisms underlying CD70-mediated metastatic processes remain poorly defined. Methods CD70 was knocked out or overexpressed in three ccRCC cell lines, and its role in tumor progression was assessed using in vitro migration and invasion assays, as well as in vivo tumorigenesis and metastasis models . Mass spectrometry-based proteomics was performed to identify differentially expressed proteins and potential pathway dysregulation following CD70 depletion. Co-immunoprecipitation (Co-IP) assays confirmed the CD70-NF1 interaction, and NF1 knockdown in CD70-knockout cells was conducted to reverse the effects of CD70 depletion on Ras/ERK signaling, migration, invasion, and epithelial-mesenchymal transition (EMT)-related features. Expression of EMT-related marks were evaluated by quantitative real-time PCR (qRT-PCR), Western blot, and immunohistochemistry (IHC). Results CD70 was significantly upregulated in ccRCC tissues and correlated with poor survival outcomes. CD70 knockout markedly inhibited in vitro cell migration and invasion, as well as in vivo tumor growth and metastasis. Mechanistically, CD70 depletion was associated with increased neurofibromin 1 (NF1) expression in ccRCC cells; CD70 knockout resulted in robust NF1 induction, which in turn suppressed Ras/ERK signaling and downstream EMT-related molecular alterations. Notably, NF1 knockdown largely reversed the inhibitory effects of CD70 knockout on these key molecular and functional phenotype changes. Conclusions Our findings uncover a novel CD70-NF1-Ras/ERK axis that contributes to ccRCC progression and metastasis, involving suppression of NF1 and subsequent activation of the Ras/ERK signaling pathway, which further promotes EMT and enhances metastatic capacity. These insights provide a mechanistic foundation for targeting CD70 as a potential therapeutic strategy in advanced ccRCC. Notably, we propose that CD70-targeted therapy may be more effective when combined with agents blocking adaptive escape pathways (e.g., Ras/ERK inhibitors), a hypothesis generated by mechanistic rescue and pharmacologic inhibition in vitro, which require further preclinical validation to confirm synergistic efficacy and clinical viability.
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