Italian Journal of Pediatrics
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2025
·
IF 3.1
Feiyu Yan,Shengnan Wang,Yilin Wang,Yan Sun,Jing Yang,Lirong Sun,Yekaterina Y Zaytseva,Pan Deng,Lingzhen Wang
Background Acute lymphoblastic leukemia (ALL) is a prevalent hematologic malignancy that primarily affects children. The diagnosis and treatment of pediatric ALL remain challenging. This study aimed to identify differential lipids and metabolites that may hold potential for improving ALL treatment. Methods In this retrospective case-control study, serum samples obtained from children with ALL and healthy controls were analyzed. Serum lipidome and metabolome alterations of ALL were analyzed by comparing pediatric patients with ALL with healthy controls based on liquid chromatography high-resolution mass spectrometry analysis of serum lipidomic and metabolomic signatures. Results We identified 2,298 lipid features in the serum. Among them, 72 (3.13%) differed significantly in pediatric patients with ALL compared to healthy controls. Notably, sphingolipids (ceramide and sphingomyelin) and phospholipids exhibited the most pronounced changes. Targeted analysis of ceramides revealed significantly elevated levels of Cer 18:0 and Cer 20:0 in the serum of pediatric patients with ALL. Additionally, gut microbial-related lipids (such as sulfonolipids and fatty acid esters of hydroxy fatty acids) showed significant alterations. Metabolomic analysis identified 15 differential metabolites, indicating disrupted nucleotide and amino acid metabolism. Furthermore, the dysregulated lipids and metabolites correlated with various blood indicators, with ceramide and nucleosides positively associated with white blood cell count but negatively correlated with hemoglobin and platelet. Conclusion These findings shed light on abnormal molecular signatures contributing to pediatric ALL and may serve as potential biomarker panel for therapy of ALL.
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高影响因子
IONICS
·
2025
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IF 2.6
Chen Peng,Wang Yan,Cai Wangfeng
In this study, we successfully synthesized a novel flower-like composite V 2 O 5 @CoV-LDH, which was directly anchored onto nickel foam through a two-step hydrothermal method to enhance surface area and stability. The synthesized flower-like composite V 2 O 5 @CoV-LDH features V 2 O 5 as the first layer and CoV-LDH as the second layer, effectively merging the high capacitance and energy density of V 2 O 5 with the large surface area and multivalent states of CoV-LDH. Moreover, this unique bilayer composite structure, directly anchored onto nickel foam, significantly enhances the overall stability of the electrode material. This design demonstrates outstanding performancedue to a strong synergistic effect, which provides the composite with more reactive sites, shorter ion transport pathways, and enhanced cycling stability. The test results show that the V 2 O 5 @CoV-LDH electrode achieves a high specific capacitance of 1822 F g −1 at 1 A g −1 and retains 85.99% of its capacitance after 5000 charge–discharge cycles, demonstrating excellent stability. The asymmetric supercapacitor assembled with the V 2 O 5 @CoV-LDH electrode and activated carbon electrode delivers an energy density of 59.85 Wh kg −1 at a power density of 900 W kg −1 . After 10,000 cycles at 5 A g −1 , the specific capacitance remains at 88%, indicating promising potential for practical applications.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 6
·1-10
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IF 5.3
Ren Dongxia,Wang Lin,Wei Hua,Mu Shijie,Yang Longfei
Bacterial infection is one of the leading causes of human death globally and is considered to be an urgent public health challenge. It is important to accurately and rapidly detect and kill pathogenic microorganisms. Therefore, a multifunctional platform for sensitive detection and efficient elimination of pathogenic bacteria should be developed to reduce the threat of bacteria. In this study, an integrated strategy based on Fe 3 O 4 -quaternary ammonium chitosan-polyetherimide-Cu-aptamer (Fe 3 O 4 -QCS-PEI-Cu-aptamer) and ladder-branch hybridization chain reaction (HCR) was proposed for the detection and inactivation of Staphylococcus aureus ( S. aureus ). The aptamer-complementary chain (aptamer-CP) conjugated to the surface of Fe 3 O 4 -QCS-PEI-Cu induces CP release in the presence of S. aureus , further initiating the ladder-branch HCR reaction and achieving signal amplification. Under optimal conditions, the developed sensor demonstrated a limit of detection (LOD) of 3 CFU/mL for S. aureus and exhibited high selectivity against several other pathogenic bacteria. Moreover, the Fe 3 O 4 -QCS-PEI-Cu-aptasensor showed potent antibacterial properties under near infrared (NIR) irradiation, achieving an antimicrobial efficiency of 99.946%. This strategy synergistically combined the high-sensitivity molecular detection with the antibacterial activity of novel nanomaterials activated by near infrared light, providing a new insight into the prevention of bacterial infections. Graphical
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高影响因子
PLANT CELL TISSUE AND ORGAN CULTURE
·
2025
·Vol. 161
·期 2
·1-16
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IF 2.4
Fu Fangfang,Li Haihua,Ling Li,Li Jiahe,Chen Yifei,Hu Weijie,Li Jiayu,Ou Lin,Chen Zhaoxi,Li Jun,Zhou Pengfei
Compared with callus, Catharanthus roseus (CAT) cambial meristematic cells (CMCs) exhibit significantly higher production of pharmacologically active terpenoid indole alkaloids (TIA), such as the anticancer drug vincristine and the antihypertensive drug ajmaline. Elicitors can significantly promote the biosynthesis of secondary metabolites in plant cells. However, the regulation of alkaloid biosynthesis in C. roseus CMCs by elicitors 2-Hydroxypropyl-β-cyclodextrin (H-β-CD) and 28-Homobrassinolide (HBL) is still unknown. Therefore, we established a new cambial meristematic cell line, named C. roseus CMCs-2, and investigated the effects of H-β-CD and HBL individually or in combination on the TIA content of CMCs in Erlenmeyer flasks (100, 250 and 500 mL) or in a 5-L airlift bioreactor. CMCs cultured in the bioreactor and treated with 10.0 g/L H-β-CD and 150 μg/L HBL gave the highest yields of vindoline (4.89 mg/L), catharanthine (2.49 mg/L) vinblastine (3.96 mg/L) and ajmalicine (3.87 mg/L), concentrations that were 241, 277, 165 and 110% higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. These results indicated that CMCs cultured in the bioreactor exhibited enhanced production of vindoline, catharanthine, vinblastine and ajmalicine than those cultured in flasks. Quantitative RT-PCR showed that H-β-CD and HBL up-regulated transcription levels of genes related to the biosynthesis of TIAs. These results imply that H-β-CD and HBL can boost TIA production in CMCs, thereby indicating that C. roseus CMCs have considerable potential as an industrial platform for the production of bioactive TIAs.
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高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2025
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IF 3.3
Zhao Bingshan,Micklefield Jason,Wang Yonghua,Wang Fanghua
N-trans -feruloyltyramine (FLA) is one kind of phenylpropanoid compound found in various plants. Numerous studies have confirmed that it exhibits a wide range of physiological functions, such as antioxidant, ɑ-glucosidase inhibition, and anti-inflammatory activity. However, the low content of FLA in plants greatly limits its potential use in food and pharmaceutical industries. It is, therefore, very important to establish an effective synthesis of FLA. In this study, a green and efficient method to synthesize FLA was sought using an amide bond synthetase (ABS) biocatalyst. Ten kinds of ABS enzymes, including AlCfaL from Azospirillum lipoferum , were screened as the potential biocatalysts for the production of FLA. To obtain optimum reaction conditions, the effects of various parameters on conversion of FLA were firstly evaluated. Under the optimum conditions using 1 mM N-trans -ferulic acid, 50 mM tyramine (substrate ratio of 1:50), 10 mM MgCl₂, 8 mM ATP, and 35 µM AlCfaL enzyme at 30 °C with a shaking speed of 500 r/min for 48 h, maximum conversion rate of 74% was reached. Given that the amidation reaction is mediated by relative expensive ATP, we further optimized reaction systems to incorporate an ATP recycling system consisting of a polyphosphate kinase enzyme (CHU) and an inexpensive polyphosphate (PolyP) as the phosphate donor. Response surface methodology (RSM) based on five-level, five-variable central composite design (CCD) was used to evaluate the optimal parameters for the production of FLA. The effects of AMP, PolyP, AlCfaL, CHU concentrations, and reaction time on the conversion rate of FLA were analyzed. The optimum conditions derived via RSM were 7.12 mM AMP, 5.96 mg/mL PolyP, 39.72 μM AlCfaL, 27.68 μM CHU, and a reaction time of 36 h. Validation experiments conducted under these optimized conditions yielded an actual conversion rate of 63.5%, which compared well to the maximum predicted value of 64.2%.
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高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2025
·
IF 3.2
Li Dan,Wu Xue,Li Yujia,Duan Cuicui,Ma Fumin,Li Xiaolei
To develop a ginseng vinegar in a short production time, the liquid fermentation of 9 days was carried out with the bacteria Acetobacter pasteurianus and Acetobacter ghanensis and the carbon source ethanol. The ginseng vinegar fermented with A. pasteurianus (AAB-hn) exhibited higher total flavonoid and polyphenol contents than ginseng vinegar fermented by A. ghanensis (AAB-jc), which reached 265.51 mg/L and 26.21 mg/L, respectively. 8 organic acids including oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, citric acid, succinic acid and fumaric acid were quantified by HPLC. Acetic acid was found to be the predominant organic acid in both samples. 108 volatile organic compounds (VOCs) were identified by GC-MS and GC-IMS. 28 differentially metabolites(VIP>1)including esters and acids were screened by OPLS-DA analysis. 10 key-VOCs were further confirmed by GC-O and odor activity values (OAVs ≥ 1). Nonanal, 2-methoxy-4-vinylphenol, and 2(3H)-furanone, dihydro-5-pentyl- were aroma factors unique to the two ginseng vinegars. Linalool and 1-octen-3-ol with floral and mushroom aromas were only detected in the AAB-hn, while α-terpineol and β-damascenone with anise and rose-like aromas were only discovered in the AAB-jc. E-nose and principal component analysis (PCA) can effectively differentiate different samples. AAB-jc had the highest overall score of 82.83 in the sensory evaluation. This study provided key insights into optimizing ginseng vinegar production by exploiting strain-specific metabolic capabilities for the development of functional vinegars with tailored health benefits and sensory profiles. Graphical abstract
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高影响因子
CELLULOSE
·
2025
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IF 4.8
Huang Xin,Ganie Showkat Ali,Rather Luqman Jameel,Mir Shazia Shaheen,Assiri Mohammed A.,Mady Mohamed F.,Li Qing
The incorporation of antimicrobial functionalities into cellulose represents a significant advancement in biopolymer modification, enhancing its potential applications in biomedical and food sectors. In this study, we synthesized and characterized quaternary ammonium-functionalized cellulose (Q-Cell) and its intermediates, employing a three-step modification strategy (tosylation, amination, and quaternization). Structural and functional attributes of the derivatives were elucidated using SEM, TGA, FT-IR, and NMR ( 1 H and 13 C) spectroscopy. The optimized synthesis under an aqueous alkaline medium (H₂O/NaOH/triethylamine) at 5 °C for 5 h enabled selective functionalization while preserving the cellulose backbone integrity. A thorough assessment of the structural characterization and stability of the components revealed that the modified cellulose demonstrated superior antibacterial and antioxidant properties accompanied with enhanced thermal properties. The antimicrobial assessment revealed 100% inhibition of E. coli and S. aureus at 250 µg/L with MIC values of 4 and 8 µg/mL, respectively. Additionally, Q-Cell exhibited superior free radical-scavenging ability in superoxide, hydroxyl, and DPPH assays, attributed to electrostatic stabilization of radicals and charge-assisted hydrogen transfer mechanisms. These findings suggest that Q-Cell is a promising candidate for antimicrobial and antioxidant applications in biomedical, pharmaceutical, and food packaging industries.
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高影响因子
Journal of Food Measurement and Characterization
·
2025
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IF 3.3
Ren Shanshan,Yu Zhenni,Wu Xue,Chen Xianggui,Wang Qin,Wang Lijun,Yang Xiao
Whey protein isolate (WPI), as a common emulsifier, is sensitive to environment conditions, reducing the multilayer emulsion stability. The covalent binding between WPI and polyphenols is commonly used to improve the physicochemical properties of WPI, and further improve the performance of multilayer emulsions. However, minimal studies are available that systematically examine the effect of different polyphenol-WPI complexes on the performance of multilayer emulsions. This study used three common polyphenols (i.e. luteolin, epigallocatechin gallate (EGCG), and rutin) for covalent coupling to WPI. The degree of covalent coupling of WPI and polyphenols was determined, suggesting the degree of covalent binding was EGCG > luteolin > rutin. Fourier transform infrared spectroscopy confirmed the potential coupling sites of WPI-polyphenol complexes. Fluorescence spectrum and circular dichroism spectrum determined changes in WPI structures. Among three WPI-polyphenol complexes, the WPI-EGCG (WPI-E) complexes displayed the best physicochemical characteristics, including emulsification property, antioxidant property, and thermal property. Then, the WPI-polyphenols complexes were used to fabricate multilayer emulsions for caffeic acid phenethyl ester (CAPE) encapsulation. The WPI-E complexes stabilized multilayer emulsion demonstrated the highest stability on pH, ionic strength, and temperature. The WPI-luteolin (WPI-L) stabilized multilayer emulsion presented the highest encapsulation rate, while the non-covalent complex WPI stabilized multilayer emulsion demonstrated the highest release rate during simulated in vitro digestion. This study helped us to better understand the structure-function relationships associated with the fabrication of multilayer emulsions from polyphenols.
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高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2025
·
IF 3.5
Hu Lan,Zhao Qiuyue,Zhang Xinling,Li Huipeng,Bian Cheng,Zeng Yiqing
Redox sites and acidic sites are the main active sites of bimetallic oxide catalysts used for selective catalytic oxidation of NH 3 (NH 3 -SCO). In this work, three MMoO x (M = Cr, Fe, and Mn) bimetallic oxides catalysts were prepared by simple grinding and calcination to investigate the effect of redox sites (M sites) on the NH 3 -SCO performance. The NH 3 -SCO test results showed that the activity of MMoO x is in the order of CrMoO x > MnMoO x > FeMoO x . The characterization results of H 2 -TPR, O 2 -TPD, NH 3 -TPD, and in situ DRIFTS proposed that the NH 3 -SCO reaction on MMoO x catalysts is proceed via i-SCR mechanism, and the formation of the intermediates of NH 3 -SCR reaction is the decisive reaction step. Owing to the excellent ability in promoting the formation of nitrate/nitrite species, CrMoO x and MnMoO x samples show much higher NH 3 -SCO activity than FeMoO x sample . CrMoO x can facilitate the formation of a large amount of –NH 2 species, which together with nitrate/nitrite species enhance the NH 3 -SCO reaction via i-SCR reaction route. Therefore, CrMoO x shows the highest NH 3 -SCO activity among the MMoO x catalysts. Through this work, the redox sites of bimetallic oxide catalysts are demonstrated as a vital role in inducing the formation of reaction-active intermediates, which highlight the importance of choosing the redox sites in designing NH 3 -SCO catalyst.
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高影响因子
Cancer Cell International
·
2025
·Vol. 25
·期 1
·1-10
·
IF 6
Zhang Hao,Liu Guoyan
GSDMB, a key protein in pyroptosis, is closely linked to various diseases. Nanomaterials can target and regulate specific pathways. Herein, we report a simple and efficient one-step method to develop multi-enzyme nanoparticles capable of suppressing GSDMB expression. 0.5ZnO@Cu5.4O can inhibit GSDMB, a capability that neither ZnO nor Cu5.4O possesses. ZnO@Cu5.4O with different ratios also exhibits varying inhibitory effects. The 0.5ZnO@Cu5.4O can be cleared quickly in vivo, ensuring biocompatibility. Additionally, 0.5ZnO@Cu5.4O significantly inhibits tumor progression, offering promising avenues for clinical cancer therapy, and the development of gene-regulating nanoparticles.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2025
·
IF 3.9
Li Huaxin,Luo Ziqiang,Xiang Zhi,Li Yafei,Shi Chen,Lu Chuanyang,Yang Jianguo,He Yanming
The preparation of N-doped conductive SiC ceramics through conventional solid-state sintering typically requires extremely high temperatures and additional nitrogen solid solution treatments. To overcome this limitation, pre-synthesized N-doped SiC nanopowder containing 1 wt.% B 4 C were used to fabricate solid-sintered conductive SiC ceramics via spark plasma sintering (SPS) at sintering temperatures of 1850–1900 °C and holding times of 1–10 min. Localized overheating during SPS induced the formation of B-Si–O-C-N liquid phase within the ceramic matrix, a phenomenon not observed in conventional solid-state sintering processes, which significantly promoted densification. Notably, short holding times (1–5 min) preserved the liquid phase, resulting in incomplete solid-state sintering. In contrast, a prolonged holding time (10 min) led to complete decomposition and evaporation of the liquid phase, thereby inducing pores formation. The fracture toughness of the resulting ceramics exceeded 4 MPa·m 1/2 , outperforming those produced via traditional routes. Additionally, the N concentration remained below 1000 ppm, while the electrical resistivity ranged from 2.68 × 10 –1 to 4.12 × 10 –2 Ω·cm, well below the 100 Ω·cm threshold required for electrical discharge machining. Furthermore, competitive doping between N and B atoms modulated the carrier concentration and mobility, enabling effective tuning of electrical resistivity. Based on these findings, the densification mechanism and doping interaction effects were thoroughly elucidated. This work provides a foundation for optimizing the solid-state sintering process and designing high-performance conductive ceramics for advanced engineering applications.
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高影响因子
Journal of Material Cycles and Waste Management
·
2024
·
IF 2.7
Zheng Zhengqiang,Weng Changzhou,Kang Zeyu,Zhong Minhua,Yu Changyong,Lin Zhang,Liu Weizhen
In China, large amounts of industrial byproduct gypsum (IBG) end up as waste and excessive stockpiling due to its low whiteness and high impurity content. In this study, two typical IBG including phosphogypsum (PG) and flue gas desulfurization gypsum (FGDG) were the research object. The impurities removal and the whiteness improvement by the tandem pyro-hydro process were investigated. Species of impurities, mechanism of whiteness improvement and the evolution of the gypsum phase during the tandem process were revealed. The results indicate that the impurities responsible for the poor whiteness of IBG included organic matter, carbon particles, and silicates. The temperature of the pyro process is the critical factor. The IBG is calcinated at 400–500 °C to fully remove organic matter and carbon particles, while decompose silicates to silica. Following, in the hydro step, the calcined IBG was treated with dilute sulfuric acid (0.2–1.0 M) to remove ion impurities such as Fe(III), the higher temperature and higher acidity are favorable to the production of valuable CaSO 4 . After treatment, the whiteness of IBG was improved from below 40% to above 80%, and the soluble impurity content can meet the standards. Moreover, both PG and FGDG products exhibited a compressive strength exceeding 10.0 MPa of curing for 7 d. This work provides theoretical guidance to promote the resource utilization of IBG, especially as plastering materials after purification and whitening. Graphical Abstract
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危险品化学品经营许可证（带存储）