Acta Neuropathologica Communications
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2026
·Vol. 14
·期 1
·122
·
IF 6.5
Zhang Xiangyi,Zhang Jingjing,Zhang Yan,Wang Dan,Liu Gaixiu,Wang Mengfei,Li Chaoyang,Shi Qi,Dong Xiaoping,Yuan Chonggang,Li Wenlong,Ma Jiyan
Prion protein (PrP) is essential for the pathogenicity of prion diseases, a group of fatal neurodegenerative disorders affecting both humans and animals. The octapeptide repeats (OR) of PrP is a highly conserved structural feature; however, conflicting observations—that OR expansion causes inherited prion diseases while being dispensable for prion transmission—obscure its role in these disorders. We developed ΔOR mice by deleting the OR from the endogenous PrP-encoding gene, PRNP , which resulted in significantly prolonged survival times for mice inoculated with any of the five prion strains. Although disease characteristics were similar between terminally ill ΔOR and wild-type mice, the aggregation and conversion of PrP to the misfolded PrP Sc were substantially delayed in ΔOR mice. This delay is attributable to reduced OR-OR self-association and diminished interactions between OR and the positively charged N-terminus of PrP. Additionally, the neurotoxic phase of the disease was both delayed and significantly prolonged in ΔOR mice. Our findings demonstrate the critical roles of OR in both PrP misfolding and neurotoxicity in prion diseases, highlighting OR as a promising therapeutic target that can mitigate both essential pathogenic processes in prion diseases.
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Journal of Bionic Engineering
·
2026
·
IF 4.4
Liu Qiang
Friction electric nanogenerators based on natural biomaterials have become a research hotspot in sports tactile monitoring since their self-powering ability, biocompatibility, and flexibility. To design a green, flexible, and high-performance tactile sensor device, this study innovatively used a lithium chloride/N-methylpyrrolidone solvent system to extract β-chitin from discarded squid cartilage. A composite nanofiber membrane was constructed using electrospinning method, and a cuttlefish cartilage-based β-chitin triboelectric array was successfully prepared. The experiment demonstrated that the peak output voltage of the frictional electric array at 3 Hz frequency was 117.0 V. After 5,000 cycles, the peak voltage remained at about 116.8 V, and the waveform shape was basically the same as the initial stage, with a peak decrease of less than 1.0%. Further placement of the frictional electric array at the heel of the football insole successfully monitored changes in foot pressure under different motion states. The output currents under walking, running, and sprinting conditions were 3.2 µA, 5.4 µA, and 8.6 µA, with stable signals and high discrimination. This study demonstrates the potential application of natural β-chitin materials in flexible self-powered sensors and provides a high-sensitivity, low-cost tactile monitoring solution for intelligent training in football.
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Nature Communications
·
2026
·Vol. 17
·期 1
·4225
·
IF 18.1
Wang Yu-Meng,Jia Yanyan,Wu Yu,Zhao Qiaohang,Guo Yumeng,Zhang Li,Jin Min,Zheng Yufang
Elevated prenatal androgen levels have been associated with an increased incidence of several neurodevelopmental conditions in offspring. However, the underlying mechanisms remain poorly understood. Here we showed that elevated prenatal androgens, modeled via aromatase inhibition in mice, disrupt cortical development and behavior in male offspring by activating androgen receptors (AR). AR interacts with MeCP2 to upregulate the transcription factor MEF2C, bypassing standard androgen response elements. Knocking down Mecp2 or Mef2c , or blocking AR, reversed these effects. Mecp2 duplication alone also upregulated MEF2C, driving similar neurogenesis and behavioral changes that were reversed by AR blockade. This reveals a MeCP2-mediated, non-canonical pathway by which androgens may contribute to male-biased neurodevelopmental conditions.
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Nature Microbiology
·
2026
·
IF 18.7
Xu Yuanpeng,Kong Xiaoying,Qiao Qian,Liu Changhao,Zhu Jinyi,Wang Lei,Hu Yanhao,Sun Yujing,Qiu Min,Yan Wei,Dong Suomeng,Ma Wenbo,Wang Yan,Wang Yuanchao
Extracellular vesicles (EVs) transport biologically active molecules and are known to mediate host defence against microbial pathogens, including plant fungal pathogens. However, the mechanism by which pathogens disrupt EV-dependent defence remains unclear. Here we show that Phytophthora capsici , a global crop pathogen, counteracts EV-mediated plant defence through targeted lipase activity. We show that Arabidopsis releases EVs containing tetraspanin (TET), specifically TET8- and TET9-EVs, which damage germinated spores of Phytophthora , reducing infection. As a counter-defence, Phytophthora secretes an infection-induced apoplastic lipase, Plant Extracellular Vesicle Destroyer 1 (PED1), which targets TET8- and TET9-EVs. This occurs via interaction with the EV membrane-localized protein Defective Glycosylation 1 (DGL1), which directly interacts and co-localizes with TET8 and TET9 on the EV membrane. PED1 damages TET8- and TET9-EVs through its lipase activity towards campesteryl esters, suppressing EV-mediated plant defence. Our study reveals a mechanism used by Phytophthora to counteract EV-mediated host defence.
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PubMed
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npj Heritage Science
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2026
·
IF 0
Li Zequn,Yuan Fang,Li Yuqi,Ma Tao,Pan Zhijun,Zhou Jianzhong,Li Weiqiang,Zhang Liangren
The production and trade of the pre-Qin prestigious goods of proto-porcelain wares has been riveting the attention of scholarly research for decades. In much of previous provenance studies, however, it has been commonly assumed that products of the major production center of Dongtiaoxi and Wuyishan are geochemically homogeneous. Our previous studies, however, have upset this assumption by identifying geochemical differences within the two centers. This study continues to explore the geochemical complexity by analyzing samples from outlying sites of the Longshan Cluster of the Dongtiaoxi Center. As a result, we find two additional complexities: a spatial dichotomy in body composition between the two banks of the Dongtiaoxi River and a diachronic evolution in glaze recipe. In the meantime, by comparing with the Wuyishan Center, we find a few fingerprint elements. Altogether, we build up a more rigorous framework for future proto-porcelain provenance studies.
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Food and Bioprocess Technology
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2026
·Vol. 19
·期 7
·359
·
IF 5.6
Li Yanjiao,Qi Yaoyao,Deng Zeyuan,Li Guangyan,Zhang Jinwu,Li Hongyan
Ganoderma lucidum ( G. lucidum ) spore oil is a premium functional lipid, but its safety is frequently compromised by phthalate plasticizers (PAEs). Although supercritical fluid extraction (SFE) is widely used to obtain spore oil, the co-extraction of these hazardous PAEs remains a critical bottleneck. This study introduces an integrated in situ adsorption strategy within the SFE process, coupling one-step micro-interfacial purification with an AHP-CRITIC multi-criteria decision model to mathematically optimize the inherent yield-quality trade-off. The application of activated carbon (AC) and activated clay (ACLT) revealed distinct physicochemical trade-offs. For AC, its non-selective microporous network caused massive competitive physical entrapment, depleting above 70% of bioactive ergosterol despite achieving a high PAEs removal rate (77.18%). Conversely, while ACLT excellently captured PAEs via specific surface chemisorption, excessive dosages introduced abundant acidic active sites that catalyzed detrimental triglyceride hydrolysis, increasing the acid value to 22.43 mg/g. Pearson correlation analysis further corroborated these inherent conflicts, revealing a powerful co-extraction dynamic between lipophilic PAEs and ergosterol ( r = 0.879**) alongside profound negative correlations between elevated AV and bioactive retention. The AHP-CRITIC model identified 0.50 kg of ACLT as the optimal adsorbent dosage, achieving the highest comprehensive score of 80.42. Under this condition, PAEs removal reached 65.70%, while above 60% of thermosensitive ergosterol was retained and oxidative stability remained excellent. Ultimately, this integrated solvent-free method offers a practical industrial alternative for refining premium functional lipids.
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Journal of Translational Medicine
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2026
·Vol. 24
·期 1
·703
·
IF 9.7
Wang Fang,Ma Shuai,Liu Zhihui,Feng Mengzhao,Song Dengpan,Li Mengyuan,An Yuan,Zhang Kaiyuan,Du Yan,Hu Shaoshan,Guo Xinbin,Guo Fuyou,Liu Han
Background Glioma is a highly aggressive brain tumor with a poor prognosis. Photodynamic therapy (PDT) induces antitumor immunity, a key mechanism of its efficacy. Dendritic cell-derived exosomes (Dexs) are crucial for tumor antigen presentation and T-cell activation. Dexs represent a promising cell-free strategy for cancer immunotherapy. Understanding the role of Dexs in PDT-induced immunity may enhance PDT efficacy. Methods GL261 glioma cells were subjected to PDT, followed by co-culture with dendritic cells (DC) to obtain PDT-Dexs. Flow cytometry and ELISA were used to assess the effects of PDT-Dexs on DC maturation and T-cell activation. MicroRNA sequencing was performed on PDT-Dexs. The effect of PDT-Dexs on tumor growth and survival was evaluated in immune-competent and immune-deficient glioma model mice. Results Co-incubation with PDT-Dexs significantly increased DC maturation and T-cell activation. Sequencing revealed 156 up-regulated microRNAs, miR-152-3p was validated as a key functional miRNA that promotes DC maturation by targeting DNMT1 to upregulate MyD88 expression. PDT-Dexs entered the systemic circulation, increasing the serum IL-2, IFN-γ, and TNF-α levels. In immunocompetent mice, PDT-Dexs increased CD3 + CD4+ and CD3 + CD8+ T-cell percentages, slowed tumor growth, prolonged survival, and were associated with enhanced CD8 + T-cell infiltration and tumor cell apoptosis. Crucially, PDT-Dexs failed to inhibit tumor growth or improve survival in immunodeficient nude mice. Conclusions PDT-Dexs promote DC maturation and T-cell activation, improving antitumor immunity and PDT efficacy. Combining of PDT with PDT-Dexs further improves antitumor immunity and PDT efficacy. PDT-Dexs warrants further investigation as a potential strategy for enhancing antitumor immunity in glioma. Graphical The alternative text for this image may have been generated using AI.
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Rice
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2026
·
IF 5.8
Jianhui Liu,Zongqiong Zhang,Baoxuan Nong,Yu Zeng,Xiuzhong Xia,Yongcheng Li,Yunjie Zheng,Yaqian Wu,Shuangshuang Luo,Zuyu Liao,Hui Guo,Xinghai Yang,Danting Li
WRKY transcription factors are important plant regulators that participate in diverse biotic and abiotic stress responses. However, their roles in resistance to Southern rice black-streaked dwarf virus (SRBSDV) remain unknown. Previous work demonstrated that the histone deacetylase gene OsHDA706 (LOC_Os06g37420) enhances SRBSDV resistance by promoting jasmonic acid (JA) biosynthesis. In this study, we first confirmed that the rice line GR216 exhibits strong resistance to SRBSDV through artificial inoculation. Knockout of OsHDA706 in GR216 led to typical SRBSDV symptoms, including dwarfing, dark-green wrinkled leaves, and inverted fibrous roots, confirming its role in antiviral defense. Using the RiceTFtarget database and yeast one-hybrid experiments, we identified OsWRKY30 (LOC_Os08g38990) as a potential upstream regulator of OsHDA706. Co-expression analysis based on the CARFI-Rice database revealed that the expression pattern of OsWRKY30 closely resembles that of several disease-resistance-related WRKY genes under biotic stress and hormone treatments. CRISPR-Cas9 knockout of OsWRKY30 in GR216 resulted in strong symptoms after inoculation with SRBSDV, supporting its involvement in antiviral immunity. RNA sequence (RNA-seq) analysis further showed that OsWRKY30 expression was significantly down-regulated in mutants after SRBSDV infection, and that defense response, stress response, and salicylic acid signaling pathways were significantly enriched in both wild-type and mutant plants. Hormone quantification revealed that JA levels were markedly higher in wild-type than in oswrky30 mutants. Collectively, these results demonstrate that OsWRKY30 positively regulates rice resistance to SRBSDV, likely by modulating JA biosynthesis. This study provides new insights into the molecular mechanisms of rice antiviral defense and offers valuable genetic resources for breeding SRBSDV-resistant cultivars.
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PubMed
高影响因子
NATURE
·
2026
·Vol. 654
·期 8118
·375-382
·
IF 56.1
Liu Shaocheng,Pols Mike,Zhang Zhongyang,Huang Xudan,Huang Zijian,Han Ying,Guo Zhenyu,Li Liang,Fan Rundong,Zhang De-Yi,Ping Du,Yu Wenjin,Gu Jiazhen,Men Luxuan,Dong Hao,Tao Shuxia,Wang Lifen,Chen Qi,Wang Huan,Xu Huaiyu,Sun Ling-Dong,Yan Chun-Hua,Zhou Huanping
Metal halide perovskites have emerged as promising semiconductors for light-emitting diodes (LEDs) owing to their excellent luminescence properties 1 . However, their performance remains limited, primarily owing to the inherent contradiction between ‘high crystallinity’ and ‘small size’ in the in situ synthesis of perovskite nanocrystals on substrates. Here we report efficient blue perovskite LEDs (PeLEDs) achieved via in situ polymerization-driven nanocrystal confinement to synthesize perovskite films composed of high-quality nanocrystals. The in situ-formed polymer network imposes nanoscale spatial constraints during perovskite nanocrystal growth, enabling nanocrystals with small sizes and a high photoluminescence quantum yield of 83%. Furthermore, polymerizable monomers with sufficient coordination sites allow a prolonged lattice rearrangement of perovskite clusters, promoting the crystallinity of the nanocrystals. The synthesized perovskite nanocrystals are utilized in the fabrication of PeLEDs, resulting in an external quantum efficiency of 21.8% at 491 nm, which is among the highest performances in blue PeLEDs. This work simultaneously controls the thermal dynamics of perovskite crystallization and organic ligand reactions, which helps to advance understanding of the effect of ligand engineering on nanocrystal synthesis, benefiting the development of efficient PeLEDs and other optoelectronic technologies.
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Nature Communications
·
2026
·
IF 18.1
Wu Fan,An Yanxin,Zhao Yuqing,Yu Jiazhi,Wu Gaoyi,Tay Franklin R.,Jiao Yang,Wang Jing
Reconstruction of large segmental bone defects remains challenging because current grafting strategies often fail to coordinate angiogenesis, neurogenesis, and osteogenesis. Here we developed a functional scaffold (peptide/Talin1 plasmid/PLA–HA/GelMA, PTPG) capable of simultaneously delivering peptides and Talin1 plasmids. We hypothesized that this scaffold enables neurovascularized bone regeneration through bidirectional activation of integrin β1 (ITGB1). The REDV–IKVAV (Arg-Glu-Asp-Val-Gly-Gly-Gly-Ile-Lys-Val-Ala-Val) peptide triggers “outside-in” ITGB1 signaling in endothelial and Schwann cells, while Talin1 plasmid-mediated “inside-out” activation. This PTPG scaffold synergistically enhances cell proliferation, migration and secretion, which are eliminated by ITGB1 silencing. In vivo, PTPG scaffold promotes aligned neurovascular networks guiding bone deposition. Single-cell RNA sequencing demonstrates enrichment of endothelial H-type signatures and repair-associated Schwann cell phenotypes, with activation of ITGB1–focal adhesion kinase–paxillin signaling. Collectively, this scaffold integrates structural support with peptide and genetic cues to promote coordinated angiogenesis, neurogenesis, and osteogenesis, offering a promising strategy for functional bone regeneration.
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Nature Communications
·
2026
·
IF 18.1
Liu Shujie,Zhao Sheng,Sun Qi,Liu Quanyi,Zhang Yihong,Li Yixuan,Jiang Lifeng,Li Tong,Cao Jianan,Wang Jiansen,Zhao Jingyuan,Cui Xiaomiao,Zhang Jingjing,Ren Xinkun,Du Yan,Zheng Liming,Jiang Qing,Wei Hui
Wear particle-induced osteolysis (WPO), primarily caused by particles generated from implants under mechanical loading, remains a major barrier to the long-term success of prosthetic implants. Traditional strategies to address WPO have largely focused on reducing wear generation, rather than mitigating the immunostimulatory effects elicited by wear particles. However, the limited scope of mechanical enhancement and the inevitable generation of wear particles shift increasing attention toward biological improvements. Herein, we propose a “proactive preventive” strategy for liner design, aiming to effectively prevent WPO by simultaneously reducing the generation of wear particles and minimizing their immunostimulatory effects. To validate the feasibility of this strategy, we synthesize a model system by incorporating ceria nanozyme into ultra-high molecular weight polyethylene (UHMWPE), referred to as CZPE. Mechanical assessments demonstrate an approximately 32% reduction in wear particle production of CZPE compared with pristine UHMWPE. Moreover, CZPE particles elicit a markedly attenuated immune response compared to PE particles in a male murine model of WPO, including reduced macrophage-mediated inflammation and diminished intramedullary plasma cell infiltration. These effects contribute to a reduction in osteoclastic bone resorption and foreign body reactions. Leveraging these mechanical and biological advantages, our approach offers a promising implant design strategy for WPO prevention.
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CELLULAR AND MOLECULAR LIFE SCIENCES
·
2026
·
IF 6.5
Siqi Nian,Lulu Zhao,Xiaokang He,Ping Xia,Yanqing Liu,Baotong Hua,Jun Li,Hongxing Zhang
Background: Patients with heart failure (HF) frequently develop atrial fibrillation, but the precise molecular mechanisms are unclear, leading to limited therapeutic strategies. Methods: Isoproterenol hydrochloride (ISO)-induced mouse HF model and ISO-treated HL-1 cardiomyocyte model were employed. A combination of qRT-PCR, Western blot, ChIP, dual-luciferase reporter assay, MeRIP-qPCR, RIP-qPCR, actinomycin D assay, and mitochondrial function assessments were used to systematically investigate the regulatory mechanism of the ALYREF/MZF1/HTRA1 axis and its role in cardiac remodeling and atrial fibrillation susceptibility. Results: HTRA1 expression was elevated in HF mice and human atrial fibrillation data. In cellular models, HTRA1 overexpression induced mitochondrial damage, cellular hypertrophy, and inflammation via the HIF-1α pathway. The transcription factor MZF1 directly bound to and activated the HTRA1 promoter. NSUN2-mediated m5C modification of MZF1 mRNA was required for its recognition and stabilization by the m5C reader protein ALYREF, thereby positively regulating the MZF1/HTRA1 axis. In the ISO-induced HF mouse model, knockdown of ALYREF effectively reduced MZF1 and HTRA1 expression, alleviated myocardial hypertrophy, fibrosis, cardiac dysfunction, and atrial fibrillation inducibility. Conclusion: This study elucidates a novel signaling axis wherein NSUN2-mediated m5C modification of MZF1 mRNA enables its stabilization by ALYREF, leading to transcriptional upregulation of HTRA1. This axis drives cardiomyocyte mitochondrial dysfunction, hypertrophy, and inflammation in the context of HF, ultimately increasing susceptibility to atrial fibrillation.
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