SCIENCE OF THE TOTAL ENVIRONMENT
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2024
·
IF 8.2
Shulin Liu,Muhammad Junaid,Chong Wang,Jun Wang
Nanoplastics (NPs, < 1000 nm) interact with chemicals and biomolecules to produce chemical−/eco-corona, altering the environmental destiny, bioavailability, and toxicity of plastic particles and co-occurring chemicals. This study employs exogenous (humic acid, HA) and endogenous (bovine serum albumin, BSA) natural organic matter (NOM) to investigate the eco-corona formation on NPs and explore the interfacial effects of eco-corona and 6:2 chlorinated polyfluorinated ether sulfonate (Cl-PFESA, commonly named as F-53B) on zebrafish ( Danio rerio ) after 7 days of exposure. Our results indicated significant changes in growth and developmental indices of zebrafish embryos among all eco-corona groups ( p < 0.05). Additionally, NFB (BSA-corona, 1 mg/L NPs + 200 μg/L F-53B + 10 mg/L BSA), NFH (HA-corona, 1 mg/L NPs + 200 μg/L F-53B + 10 mg/L HA) and NFHB (BSA-HA-corona, 1 mg/L NPs + 200 μg/L F-53B + 10 mg/L BSA + 10 mg/L HA) showed elevated bioaccumulation of NPs, ROS generation and induction of apoptosis. Transcriptomic analysis showed the number of differentially expressed genes (DEGs) in the following order: BSA-HA-corona (NFHB (2953) > HA-corona (NFH (2797) > NH (2721) > F-53B (2292) > NF (2033) > BSA-corona (NFB (687) > NB (450)), and no DEGs were detected in the single NP compared to the control. Further, the PI3K-AKT, immune system, endocrine system, digestive system, infectious diseases, and neurovegetative disease pathways showed sensitive responses in the NFH/NFHB groups compared to those in the NFB group. Therefore, the interactive effects of NPs and F-53B on zebrafish embryos were lower in the presence of BSA-corona than those in HA- or HA-BSA-coronas, indicating a relationship between the formation of diverse eco-coronas on NPs by multiple NOM and an associated increase in the interfacial toxicological effects of plastic particles and F-35B in freshwater organisms.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2024
·
IF 4.2
Maoshan Wang,Yingxue Liu,Houda Gui,Gaoqiang Ma,Binyang Li,Zhanwei Zhang,Gyeonghwi Yu,Ailin Wu,Xin Xu,Dongjiao Zhang
Eldecalcitol (ED-71), a novel active form of vitamin D, shows potential in treating osteoporosis. However, its underlying mechanisms of action remain to be determined. This study aimed to investigate the effect of ED-71 on bone regeneration and to illustrate its mode of action. The in-vitro model was developed using rat primary osteoblasts cultured under high-glucose conditions, and these cells were treated with ED-71. Additionally, an in vivo model of cranial bone defects was established in type 2 diabetic rats, and ED-71 was administered by gavage. The results demonstrated that ED-71 prevented osteoblast cell death, enhanced rat primary osteoblasts' osteogenic capacity, and attenuated the overexpression of hypoxia-inducible factor 1α (HIF1α) induced by high glucose levels. Furthermore, ED-71 increased glutathione peroxidase 4 (GPX4) levels and inhibited ferroptosis in response to hyperglycemic stimulation. Notably, interference with the HIF1α activator and ferroptosis activator Erastin significantly reduced the therapeutic effects of edetate osteolysis. These findings were further tested in vivo experiments. These results suggest that ED-71 activates the HIF1α pathway in vivo and in vitro, effectively relieving the ferroptosis induced by high glucose. Significantly, ED-71 may improve osteogenic disorders caused by diabetes.
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
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2024
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IF 6.9
Linquan Dai,Bo Feng,Mengyan Tang,Yuangan Chen
It is challenging to separate between molybdenite and chalcopyrite due to their comparable hydrophobic characteristics. Inorganic depressants were previously used to separate two minerals, but the results were unsatisfactory and polluted the environment. In this study, the molybdenite and chalcopyrite were separated using a highly effective and ecologically safe depressant, 2,4-Diamino-6-hydroxypyrimidine (DAHP). According to the single mineral experiment, chalcopyrite is totally depressed while molybdenite has superior floatability. The efficacy of DAHP as the depressant was validated by further artificially mixed mineral flotation experiments, indicating the possibility of separating chalcopyrite and molybdenite. The adsorption capacity experiment results showed that the adsorption capacity of DAHP on the surface of chalcopyrite was greater than that on the surface of molybdenite. Zeta potential experiment results show that DAHP is adsorbed on the surface of chalcopyrite and molybdenite through static electrostatic interactions . X-ray photoelectron spectroscopy (XPS) experiment results show that DAHP is mainly adsorbed on chalcopyrite surface through –NH 3 + group, which hinders the adsorption of kerosene. The electrostatic contact between DAHP and molybdenite has the least effect on kerosene adsorption. This study shows that DAHP has the potential to separate chalcopyrite and molybdenite as an efficient and environmentally friendly depressant.
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CytoJournal
·
2024
·
IF 2.5
Yan Li,Zhenwei Jia,Xiaoyang Kong,Hongbo Zhao,Xiaoyan Liu,Guirong Cui,Jianmin Luo
Objective:
We currently face a sharp increase of T-cell acute lymphoblastic leukemia (T-ALL) incidence and a challenge of unmasking its complex etiology. The deoxycytidine analog 5-Aza-2’-deoxycytidine (5-Aza-dC) is currently the most common nucleoside methyltransferase inhibitor. The objective of this study was to clarify the role of 5-Aza-dC in T-ALL cell biological behaviors and phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression.
Material and Methods:
T-ALL cell lines were divided into the experimental group with 5-Aza-dC solution treatment, and the control group without treatment. PTEN methylation was detected using methylation-specific polymerase chain reaction (MS-PCR). Following the measurement of cell proliferation, viability, apoptosis, invasion, migration, etc., quantitative reverse transcription-polymerase chain reaction (PCR) was conducted to detect PTEN, DNA methyl-transferases (DNMT1), DNMT3a, MBD2, and MeCP2 expressions; Western blot to detect PTEN, PI3K, AKT, and mTOR protein expressions. In addition, rescue experiments to inhibit and restore the expression of PTEN in different groups were performed for further identification of the results in the former parts.
Results:
MS-PCR results showed that in Jurkat cells, the target band was amplified using methylated primers for the PTEN gene promoter region; moreover, at 10 μmol/L of 5-Aza-dC for 24 h, PTEN methylation was completely removed without any un-methylated band observed. The experimental group had significantly lower cell proliferation and viability rates, higher apoptosis rates, decreased cell proportion in S phase, reduced invasion and migration; increased PTEN expression, decreased DNMT1, DNMT3a, MBD2, and MeCP2 mRNA expressions; and decreased PI3K, AKT, and mTOR protein expressions than those in the control group (all P < 0.05). Furthermore, according to the rescue experiment, silenced PTEN expression weakened the beneficial roles of 5-Aza-dC treatment, and resulted in significantly higher cell proliferation and viability rates, lower apoptosis rates, increased cell proportion in S phase, increased cell invasion and migration; decreased PTEN expression, elevated DNMT1, DNMT3a, MBD2, and MeCP2 mRNA expressions, and higher PI3K, AKT, and mTOR protein expressions (all P < 0.05). While restored PTEN expression enhanced functions of 5-Aza-dC treatment, leading to obviously lower cell proliferation and viability rates, higher apoptosis rates, increased cell proportion in G1 phase, and reduced cell invasion and migration; as well as increased PTEN expression, decreased DNMT1, DNMT3a, MBD2, and MeCP2 mRNA expressions, and lower PI3K, AKT, and mTOR protein expressions (all P < 0.05).
Conclusion:
Demethylation treatment with 5-Aza-dC can inhibit T-ALL cell malignant biological behaviors and enhance the sensitivity to chemotherapy agents possibly, which may be related to the inhibited expressions of DNMT1, DNMT3a, MBD2, and MeCP2, and restored expression activity of PTEN to negatively regulate the PI3K/AKT signal transduction. Our silencing and restoration of PTEN expressions further support our findings, highlighting that demethylation with 5-Aza-dC to restore the anti-tumor activity of the tumor suppressor gene PTEN may be a promising therapeutic option for treating T-ALL.
Keywords: 5-Aza-2’-deoxycytidine, T-cell acute lymphoblastic leukemia, PTEN, Tumor cell biological behaviors, Demethylation
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JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Tao Wu,Shiyu Zhang,Miaomiao Zhang,Mengying Long,Guangzhe Wang,Tianrun Gu,Hao Deng,Cuiting Ding,Runnan Zhang,Zhongyi Jiang
When introducing acid groups in the diamine monomer for the fabrication of polyamide (PA) membranes through interfacial polymerization (IP), the acid groups not only reduce the diffusion flux of diamine monomer for the polymerization to prepare ultrathin membrane but also provide the negative charges on the membrane surface . However, the introduction of acid groups often weakens the reactivity of diamine monomers, resulting in the formation of thick and loose PA membranes with low rejection of ions. This study proposes a strategy of modulating the IP process of piperazine-2-carboxylic acid (CPIP) by employing an acid acceptor (NaOH) to prepare thin nanofiltration membranes . The acid acceptor can neutralize the HCl to prevent CPIP from being protonated during the IP process, thereby facilitating the cross-linking reaction rate. The thickness of the resulting membrane decreases from 180 nm to 30 nm, accompanied by the fortification of the negative charge, an increase in cross-linking density, and a reduction in pore size. The resulting PA-AA/CPIP_1.0 membrane exhibits a water permeance of 44.6 L m −2 h −1 bar −1 , with a significantly increased Na 2 SO 4 rejection of 98.4 % compared with 50.4 % of the PA-AA/CPIP_0 membrane fabricated without acid acceptor. This work may open a new avenue to fabricating high-performance PA membranes for nanofiltration .
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Science China-Chemistry
·
2010
·Vol. 53
·期 7
·1497-1501
·
IF 0
Ma Hong,Nie Xin,Cai JiaYing,Chen Chen,Gao Jin,Miao Hong,Xu Jie
The catalytic oxidation of biomass-derived polyhydroxyl compounds provides an efficient and green route for biomass resource utility. Gold nanocrystals supported on Mg(OH) 2 were synthesized by sol-immobilization method, characterized by XRD, TEM, UV-vis DRS, and tested in the selective oxidation of 1,2-propanediol to lactic acid. The gold particle size and the ratio of Au to 1,2-propanediol strongly influenced the catalytic activity. Over Au/Mg(OH) 2 with the majority of gold particles concentrated in 14–18 nm, excellent catalytic performance with 94.4% conversion and 89.3% selectivity to lactic acid has been achieved under 0.3 MPa O 2 at 60 °C for 6 h.
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PHYTOMEDICINE
·
2025
·
IF 8.3
Feng Jia,Yongqing Wang,Zhiqiang Chen,Jingxian Jin,Lei Zeng,Li Zhang,Huaijian Tang,Yanyan Wang,Pei Fan
Background 10-Hydroxydec-2-enoic acid (10-HDA), a unique and marker compound in royal jelly, has a wide range of bio-activities. However, its role in regulating inflammation of vascular smooth muscle cell (VSMC), which is essential to a set of vascular diseases, is still unknown. Purpose Our study aimed to investigate whether 10-HDA exerts effect on VSMC inflammation via interacting with toll-like receptor 4 (TLR4), a pivotal inflammatory initiator. Methods A package of proteins, which might participate in TLR4-mediated signaling, influenced by 10-HDA were analyzed in mouse VSMCs with Angiotensin Ⅱ(Ang Ⅱ) or lipopolysaccharide (LPS) stimulation. Accordingly, pro- or anti-inflammatory cytokines, reactive oxygen species (ROS), and anti-oxidants that are closely relevant to inflammatory process were determined. The possible mode for 10-HDA interacting with TLR4 was also characterized. Moreover, involvement of a key miRNA in 10-HDA regulating VSMC inflammation was identified. Results In the presence of Ang Ⅱ, 10-HDA inhibited the TLR4 expression in a dose-dependent manner. In such occasion, 10-HDA hindered the up-regulation of specificity protein 1 (SP1) and serine/threonine-protein phosphatase 6 catalytic subunit (PPP6C), the phosphorylation of extracellular signal-regulated kinase 1/2, TGF-β-activated kinase 1, and nuclear factor-κB p56, as well as the enhancement of myeloid differentiation primary response gene 88. Apart from SP1 and PPP6C, the level change of these proteins by 10-HDA was similar with LPS stimulation. The effect might be resulted from 10-HDA blocking TLR4 through multiple atomic interactions. 10-HDA mitigated the increase of pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin-2 (IL-2), and IL-6, as well as increased the anti-inflammatory cytokine IL-10, in the Ang Ⅱ- or LPS-induced VSMCs. Correspondingly, the level of ROS was attenuated and the anti-oxidants such as glutathione and superoxide dismutase were fortified. The data indicated the anti-inflammatory potential of 10-HDA in VSMCs, which was associated with 10-HDA's capability of relieving oxidative stress. Additionally, the expression of miR-17–5p was saved by 10-HDA from Ang Ⅱ- or LPS-treated VSMCs, which might be relevant to SP1 and PPP6C targeting. Conclusion The present work of 10-HDA, for the first time, revealed its ability to alleviate VSMC inflammation by targeting TLR4 and therefore modulate the downstream inflammatory participants. Our data will cast light on the utilization of 10-HDA in VSMC inflammation-related vascular disorders.
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ANALYST
·
2011
·Vol. 136
·期 22
·4770-4773
·
IF 3.913
Ling Chen,Tingting Lou,Chunwei Yu,Qi Kang,Lingxin Chen
An approach for mercury ions (Hg2+) sensing based on the Hg2+-induced aggregation of thymine (T)–SH-functionalized gold nanoparticles (AuNPs) has been reported. The T–SH ligands that we synthesized can easily be coupled to the surface of AuNPs through the Au–S bond and can recognize Hg2+ with high selectivity by forming a T–Hg–T complex with strong affinity. For the T–SH-functionalized AuNPs (T–S-AuNPs) sensor, upon addition of Hg2+, the formation of the T–Hg–T complex induces aggregation of T–S-AuNPs and results in a significant change of color and UV-Vis absorption spectra. Thus, our method can be used for the rapid, easy and reliable screening of Hg2+ in aqueous solution, with high sensitivity (2.8 nM) and selectivity over competing analytes. The developed method is successfully applied to the sensing of Hg2+ in real environmental samples.
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Food Bioscience
·
2024
·
IF 4.8
Jiajing Liao,Jin Qian,Lijuan Rao,Suqin Lin,Chen Wang,Linqian Xu,Bing Yuan,Jiayi Yuan,Yin Wan,Guiming Fu
Hyperuricemia is a metabolic disease caused by excessive production of uric acid or abnormal excretion of uric acid. Although most of the uric acid is excreted in the kidneys, the elimination of uric acid in the intestines still plays an important role. 3,5-dicaffeoylquinic acid (3,5-diCQA) is widely found in Chrysanthemum and Artemisia species, where it inhibits the activity of xanthine oxidase (XOD), a key enzyme in uric acid production. Therefore, a Caco-2 cell model was utilized to explore the mechanisms of 3,5-diCQA affecting uric acid excretion under high uric acid conditions, focusing on aspects such as intestinal uric acid transport, the intestinal barrier, and the MAPK signaling pathway. The results showed that 3,5-diCQA could reduce the oxidative stress and apoptosis induced by high uric acid, repair the intestinal barrier, upregulate the expression of ABCG2 and MRP4, promote the excretion of uric acid and inhibit the activation of MAPK signaling pathway. In addition, 3,5-diCQA could also regulate the expression of ABCG2 and MRP4 via the MAPK signaling pathway. These findings provide scientific evidence for dietary recommendations for individuals with hyperuricemia, suggesting that foods or supplements containing 3,5-diCQA may help manage uric acid levels.
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Small
·
2025
·
IF 12.1
Shaojian Jiang,Yuhang Liu,Ruidong Yang,Lijun Zhang,Wenke Liu,Kai Deng,Hongjie Yu,Hongjing Wang,Liang Wang
Since formaldehyde oxidation reaction (FOR) can release H 2 , it is attractive to construct a bipolar hydrogen production system consisting of FOR and hydrogen evolution reaction (HER). Although copper-based catalysts have attracted much attention due to their low cost and high FOR activity, the performance enhancement mechanism lacks in-depth investigation. Here, an amorphous-crystalline catalyst of amorphous nickel hydroxide-coated copper dendrites on copper foam (Cu@Ni(OH) 2 /CF) is prepared. The modification of Ni(OH) 2 resulted in hydrophilic and aerophobic states on the Cu@Ni(OH) 2 /CF surface, facilitating the transport of liquid-phase species on the electrode surface and accelerating the release of H 2 . The Open circuit potential (OCP) and density functional theory (DFT) calculations indicate that this core–shell structure facilitates the adsorption of HCHO and OH − . In addition, the catalytic mechanism and reaction pathway of FOR are investigated through in situ FTIR and DFT calculations, and the results showed that the modification of Ni(OH) 2 lowered the energy barrier for C─H bond breaking and H─H bond formation. In the HER//FOR system, Pt/C//Cu@Ni(OH) 2 /CF can provide a current density of 0.5 A cm −2 at 0.36 V and achieve efficient and stable H 2 production. This work offers new ideas for designing electrocatalysts for bipolar hydrogen production system assisted with formaldehyde oxidation.
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LANGMUIR
·
2025
·
IF 3.9
Xihong Ding,Demeng Qian,Hui Wang,Qian Hu,Lin Zhu,Hui Chen,Tengda Xu,Aohan Li,Wei Hong
This study investigates the role of 2-aminoethanethiol hydroiodate (2-AET·HI) additives in enhancing the phase stability and photovoltaic performance of CsPbI3 films for inorganic perovskite solar cells. The incorporation of 2-AET·HI into the precursor solution results in a more uniform and compact film morphology. X-ray diffraction (XRD) analysis reveals that the 2-AET·HI-treated CsPbI3 films retain their black α-phase, even after 300 h at 25% RH and ∼25 °C, whereas the control samples show a rapid transition to the nonphotoactive δ-phase. This improvement in phase stability is reflected in the enhanced photovoltaic performance, with the 2-AET·HI-modified devices exhibiting a power conversion efficiency (PCE) of 16.7%, compared to 15.4% for the control devices. Furthermore, the 2-AET·HI-modified devices display superior long-term stability, retaining 83% of their initial PCE after 192 h in ambient conditions, in stark contrast to the unmodified devices, which degrade rapidly.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Youwei Cheng,Lian Zhu,Yaqiong Gong
Metal-organic frameworks (MOFs) are widely recognized as precursors or sacrificial templates for the synthesis of target catalysts on account of their high porosity and high active surface area . Elemental doping strategy is considered as an effective method for the synthesis of efficient and stable catalysts for electrolytic water. Therefore, in this paper, Ce-doped Ni(OH) 2 nanoparticles and Ni-MOF nanosheets composites were synthesized by electrodeposition using Ni-MOF as precursor. Catalyst Ce–Ni(OH) 2 @Ni-MOF/NF displays excellent OER performance, requiring only 193 mV and 279 mV overpotentials to realize current densities of 10 mA cm −2 and 100 mA cm −2 , respectively, while the UOR performance was very remarkable, only a potential of 1.28 V was demanded to reach a current density of 10 mA cm −2 . In addition, no obvious change in the overpotential was noticed during the stability test of 16 h, indicating good stability of Ce–Ni(OH) 2 @Ni-MOF/NF. This study provides a meaningful reference for the design of an efficient and stable environmental friendly electrocatalyst .
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