Molecular Catalysis
·
2024
·
IF 3.9
Pushuang Xing,Cong Li,Yixin Chen,Rong-Lan Zhang
PMoV 2 was encapsulated in four MOFs (MIL-101, HKUST-1, UiO-67, ZIF-8) with different cavity sizes and window sizes using a hydrothermal synthesis method. Then, a layer of mesoporous silica was coated on the surface of the MOFs to obtain four designed three-layer encapsulated catalysts 30%PMoV 2 @MOF@mSiO 2 . The physicochemical properties of the catalysts were characterized through various characterization methods, and the influence of different MOF window sizes of the four catalysts on the removal rate of four thiophene sulfides was investigated. The optimal reaction conditions were also explored for the optimal catalyst. The results indicate that under simulated fuel of 2400 ppm, 30%PMoV 2 @UiO-67@mSiO 2 owns the highest catalytic activity. Under optimal conditions, the total removal rate of four thiophene compounds was 91.52 %, and after ten cycles, the efficiency could still reach 86.24 %. This is attributed to the sufficient cavity size of UiO-67, which provides assurance for the loading of PMoV 2 and the large window size, making it possible for the smooth reaction between sulfides and PMoV 2 .
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2024
·
IF 4.4
Lei Huang,Jiayi Wang,Jieqin Yu,Mengxuan Bian,Xingdong Xiang,Guanjie Han,Weisin Chen,Ning Wang,Jun Ge,Shunyi Lu,Jian Zhang
Osteoporosis (OP) can result in slower bone regeneration than the normal condition due to abnormal oxidative stress and high levels of reactive oxygen species (ROS), a condition detrimental for bone formation, making the OP-related bone healing a significant clinical challenge. As the osteogenic differentiation ability of bone marrow mesenchymal stem cells (BMSCs) is closely related to bone regeneration; currently, this study assessed the effects of Picein on BMSCs in vitro and bone regeneration in osteoporotic bone defect in vivo. Cell viability was determined by CCK-8 assay. The production of (ROS), malonaldehyde, superoxide dismutase activities, and glutathione was evaluated by using commercially available kits, and a flow cytometry analysis was adopted to detect macrophage polarization. Osteogenic capacity of BMSCs was evaluated by alkaline phosphatase (ALP) activity, ALP staining, and Alizarin red S staining. The expression of osteogenic-related proteins (OPN, Runx-2, OCN) and osteogenic-related genes (ALP, BMP-4, COL-1, and Osterix) were evaluated by Western blotting and real-time PCR (RT-PCR). In addition, proliferation, migration ability, and angiogenic capacity of human umbilical vein endothelial cells (HUVECs) were evaluated by EdU staining, scratch test, transwell assay, and tube formation assay, respectively. Angiogenic-related genes (VEGF, vWF, CD31) were also evaluated by RT-PCR. Results showed that Picein alleviated erastin-induced oxidative stress, enhanced osteogenic differentiation capacity of BMSCs, angiogenesis of HUVECs, and protects cells against ferroptosis through Nrf2/HO-1/GPX4 axis. Moreover, Picein regulate immune microenvironment by promoting the polarization of M2 macrophages in vitro. In addition, Picein also reduce the inflammation levels and promotes bone regeneration in osteoporotic bone defect in OP rat models in vivo. Altogether, these results suggested that Picein can promote bone regeneration and alleviate oxidative stress via Nrf2/HO-1/GPX4 pathway, offering Picein as a novel antioxidant agent for treating osteoporotic bone defect.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xiu Gong,Shuhan Fan,Qu Yang,Jing-Liang Yang,Yanli chen,Xiaosi Qi,Hui Shen,Dan Ren,Mingkui Wang
Using photoelectrochemical technology to construct efficient photoelectric materials has aroused extensive research interest. In this study, we propose a two-dimensional porous CuSe@BiOI isotype heterojunction photocathode for CO 2 reduction to formic acid. The heterojunction was constructed by the epitaxial growth of high-quality oriented BiOI nanosheets onto the porous CuSe framework. The CuSe framework as a template to ensure the formation of the (1 0 2) facets of BiOI and the isotropic charge transport characteristics of the (1 0 2) surface greatly improve the efficiency of charge separation and transfer. This CuSe@BiOI isotype heterojunction electrode can efficiently reduce CO 2 to formic acid with a selectivity of up to 80.5 % in 0.5 M potassium bicarbonate aqueous solution. Meanwhile, the performance of the photodetector based on CuSe@BiOI heterostructure is 6.97 × 10 11 Jones and a response rate of 87.13 mA W −1 . This finding provides a new approach to designing stable heterojunction photoelectrocatalysts with isotropy.
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Shuxing Zhang,Xiaoshu Ding,Yanji Wang
5-Amino-1-pentanol is an important pharmaceutical intermediate used in the synthesis of anti-inflammatory and anticancer drugs. The direct hydrogenolysis of furfurylamine to 5-amino-1-pentanol has the advantages of a simple process route and high atom utilization. In this paper, for Pt/ZrO2 catalysts, the effects of different Pt loading and calcination temperatures on catalyst activity, selectivity, and surface properties such as Pt nanoparticle size, acidity and alkalinity, and the ratio of Pt0/(Pt0+Pt2+) were investigated. Through the study of the relationship between catalyst preparation conditions, furfurylamine hydrogenolysis reaction performance, and surface properties, it was found that moderate Pt nanoparticle size, higher Pt0/(Pt0+Pt2+) ratio, and more acidic sites favored furfurylamine hydrogenolysis. The higher number of basic sites favors the dispersion of Pt metal. The results of in situ FTIR show that the amino group of furfurylamine is adsorbed on the acidic site of Pt/ZrO2, and the furan ring is adsorbed on Pt nanoparticles, which is beneficial to the hydrogenolysis of furfurylamine.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Suqi Liu,Xiaowei Wu,Shihao Chen,Cong Liu,Wanqi Zhang,Mingyi Yang,Jiyu Cheng,Guoquan Lu,Zhenhe Wang,Wei Chen,Yuge Guan,Linjiang Pang,Xinghua Lu
Linalool, a terpenoid alcohol with bacteriostatic properties, was detected in sweetpotato suffering from black spot disease. In this research, a composite material based on HKUST-1/MWCNT-gel@MIP was employed to detect the linalool content in these infected sweetpotatoes. The micro-morphology and crystal structure of the composite were characterized using scanning electron microscopy (SEM), fourier transform infrared (FTIR), and x-ray diffraction (XRD). Experimental results demonstrated that the quartz crystal microbalance (QCM) gas sensor of HKUST-1/MWCNT-gel@MIP composite exhibited high sensitivity (0.873 Hz/ppm), a low detection limit (0.237 ppm), a small standard deviation (0.83 %), good reproducibility, and outstanding selectivity. The QCM gas sensor data from actual samples showed a strong correlation with gas chromatography-mass spectrometry (GC–MS) results (r = −0.976, p < 0.01), demonstrating its exceptional ability to identify linalool. Thus, it can be used for early detection of sweetpotato black spot disease.
✓ 已验证
产品关联
DOI
高影响因子
Fermentation-Basel
·
2024
·Vol. 10
·期 2
·87
·
IF 3.3
Wenjiao Xue,Chen Liu,Yao Liu,Hao Ding,Chao An,Shizhe Zhang,Saijian Ma,Qiwen Zhang
To assess the probiotic potential of strain 68-1 with rutin conversion capabilities, isolated from Chinese traditional Jiangshui, a complete genomic analysis and in vitro tests were conducted. The Oxford Nanopore Technologies (ONT, Oxford, UK)–Illumina (San Diego, CA, USA) hybrid sequencing platform was used for whole genome sequencing and the results showed that strain 68-1 had a chromosome sequence of 3,482,151 bp, with 46.53% GC content and five plasmids with a sequence length ranging from 2009 bp to 48,711 bp. Strain 68-1 was identified asLactiplantibacillus pentosusbased on the whole genome sequence. A total of 133 genes encoding for carbohydrate-active enzymes (CAZymes) were identified and genes that may be involved in rutin conversion were found in theL. pentosus68-1 genome.L. pentosus68-1 showed excellent tolerance to gastrointestinal juice and adhesion properties, and corresponding genes were identified. In addition,L. pentosus68-1 exhibited strong antibacterial and antifungal activity, where organic acids may play a crucial role in its antagonistic ability. Moreover, the gene cluster for plantaricin_EF production was detected. No high virulence factor was found in theL. pentosus68-1 genome and no hemolytic effect was observed. In addition,L. pentosus68-1 showed resistance to ampicillin, gentamycin, and kanamycin, and the genomic analysis indicated that horizontal ARG transfer should not be possible. The results show thatL. pentosus68-1 could be developed as a novel probiotic candidate to improve rutin bioavailability in the food and feed industry.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2024
·
IF 4.5
Yu-Ru Ren,Wen-Ting Cui,Kai-Li Jiang,Kai-Qi He,Yong-Ming Lu,Yan Chen,Wen-Juan Pan
Age-related cognitive decline is primarily attributed to the progressive weakening of synaptic function and loss of synapses, while age-related gut microbial dysbiosis is known to impair synaptic plasticity and cognitive behavior by metabolic alterations. To improve the health of the elderly, the protective mechanisms of Oudemansiella raphanipes polysaccharide (ORP-1) against age-related cognitive decline are investigated. The results demonstrate that ORP-1 and its gut microbiota-derived metabolites SCFAs restore a healthy gut microbial population to handle age-related gut microbiota dysbiosis mainly by increasing the abundance of beneficial bacteria Dubosiella , Clostridiales , and Prevotellaceae and reducing the abundance of harmful bacteria Desulfovibrio , strengthen intestinal barrier integrity by abolishing age-related alterations of tight junction (TJ) and mucin 2 (MUC2) proteins expression, diminish age-dependent increase in circulating inflammatory factors, ameliorate cognitive decline by reversing memory- and synaptic plasticity-related proteins levels, and restrain hyperactivation of microglia-mediated synapse engulfment and neuroinflammation. These findings expand the understanding of prebiotic–microbiota–host interactions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceuticals
·
2024
·Vol. 17
·期 3
·291
·
IF 4.3
Nannan Kang,Jun Ma,Yuling Hu,Rongrong Di,Lei Wang,Xuanling Zhang,Yisheng Lai,Yu Liu
Background: Breast cancer is a commonly diagnosed cancer worldwide. Human MutT homolog 1 (MTH1) is found to be elevated in breast tumors and cancer cells need MTH1 for survival. Pharmacological inhibition of MTH1 may be potentially beneficial in the treatment of breast cancer. Methods: MA−24 was screened by malachite green colorimetric assay for MTH1 inhibitors and the kinetic characteristics of MA−24 were assessed. The features of MA−24’s binding with MTH1 were ascertained through molecular docking, and the cytotoxic activity of MA−24 was validated in vitro and in vivo. Target engagement assays, comet assay, and Western blot confirmed the intracellular target and mechanism of MA−24. Results: MA−24 shows potent antitumor bioactivity both in vitro and in vivo. MA−24 competitively inhibited the MTH1 and further induced DNA strand breaks, leading to increased apoptosis of cancer cells depending on the upregulation of the cleaved-caspase 3–cleaved-PARP axis. In particular, MA−24 exhibited a powerful efficacy and safety in vivo (tumor growth inhibition rate: 61.8%). Conclusions: MA−24 possesses a broad spectrum of breast cancer cytotoxicity and offered valuable insights for overcoming the challenges of chemotherapy-related toxicity, which holds great potential for the further development MA−24 as an anti-cancer drug.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Exploration
·
2025
·
IF 22.5
Fangfang Liu,Zhong-Da Li,Yanqiao Zeng,Xiaofeng Wang,Yingnan Liu,Qi Li,Wenhe Luo,Xiaoman Suo,Yaqing Xu,Feng Yuan,Dan Zhang,Wuqiong Zhang,Shengyong Geng,Xue-Feng Yu,Guofang Zhang,Yang Li
The intrinsic properties of black phosphorus (BP) nanomaterials (NMs) enable them for targeted binding to polo-like kinase 1 (PLK1), thus inhibiting its kinase activity. However, the mechanism and targeted binding sites underlying this interaction remain unclear. To elucidate the critical properties of PLK1 that facilitate its interaction with BPNMs, the binding ability of BPNMs was compared across PLK family proteins. Although BPNMs exhibited a weak binding affinity for PLK3, PLK1 demonstrated the most favorable physicochemical properties for its binding. Factors as surface charge, hydrophobicity, secondary and three-dimensional structures significantly affected the interaction of PLK family proteins to BPNMs. The binding affinity was primarily determined by amino acid residues at the binding interface, where arginine and proline played critical roles in mediating the interaction of BPNMs-PLK1. BPNMs inhibited PLK1 activation by binding to key residues of the kinase domain, including S49, Y203, D204, E206, and R207. In conclusion, this study elucidates the molecular basis of the specific interaction between BPNMs and PLK1, highlighting the pivotal role of the amino acid residues in NM-protein binding. This work demonstrates that NM-protein interactions are a mutual selection and driven by the physicochemical properties of both proteins and NMs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Wenjing Li,Juan Shao,Chuxin Zhang,Guodong Cao,Yitao Yan,Qiongyi Han,Huimei Yu,Yingying Wei,Wenbo Zhang,Jie Zheng,Zhongyuan Guo
Colorectal cancer has emerged as a leading cause of global cancer-related morbidity and mortality, necessitating innovative therapeutic strategies. We developed a desilication-recrystallization method to synthesize Silicalite-1-X (X = 2, 8, 16, 32), a hierarchical porous silica molecular sieve with cost-effectiveness, facile synthesis, and tunable porosity. Its programmable mesoporous structure is achieved by precisely controlling the desilication-recrystallization kinetics. Tunable mesopores with precise diameters of 4–6 nm and 20–30 nm were fabricated by controlling cavity expansion through temporal modulation (2–32 h) of a time-dependent synthesis process. Detailed analysis of drug loading and release profiles revealed that although pore structure modification did not significantly alter drug loading efficiency (DLE), it induced a slight but consistent improvement in drug release rate as pore size increased. Systematic biocompatibility evaluation was conducted through an in vitro human colonic epithelial tissue model complemented by the KM mice in vivo studies. Histopathological analysis confirmed Silicalite-1-X caused minimal cytotoxicity and oxidative stress in healthy colon tissue, preserved mucus secretion, and showed no systemic toxicity. Furthermore, Doxorubicin (DOX) loaded Silicalite-1-X platform (Silicalite-1-DOX-X) exhibited dose-dependent cytotoxic efficacy against colorectal carcinoma in vitro models, confirming its anti-tumor therapeutic potential. Our findings provide a framework for engineering molecular sieves with tunable porosity and a platform for expanding their multifunctional applications in advanced materials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Zhenguo Zhang,Xue Li,Shuo Zhang,Mingshuang Zheng,Fangping Yuan,Shushen Li,Bei Yuan,Lisheng Liu,Xin Du
Although immunotherapy has become an established approach in cancer treatment, not all patients exhibit a positive response. Current research on PD-1 primarily focuses on immune cells using conventional methods, yet PD-1 expression on tumor cells is also widespread and plays an increasingly critical role in assessing immunotherapy efficacy. To address this, we developed an electrochemical biosensor capable of specifically detecting PD-1 at both the protein and tumor cellular levels in situ, eliminating the need for sample fixation and thereby streamlining the analytical process. Additionally, our probe enables simultaneous localization analysis of PD-1 through fluorescence signaling. The biosensor integrates a bifunctional probe composed of a nanometal–organic framework (MOF, ZIF-8) conjugated with quantum dots (QDs) and an electrode modified with a carbon nanotube (CNT)–gold nanoparticle (AuNP) nanocomposite. The morphology and performance of the nanocomposite were thoroughly characterized, and the resulting PD-1 biosensor demonstrates high specificity, reproducibility, and sensitivity. To facilitate clinical translation, we successfully applied the biosensor to detect differential PD-1 expression across various tumor cell lines and cancer tissues. This study establishes that our dual-mode electrochemical and fluorescent sensing platform provides a powerful tool for in situ PD-1 detection on tumor cells, offering valuable insights for evaluating tumor immunotherapy responses.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arthritis & Rheumatology
·
2025
·
IF 10.9
Jin-Fen Ma,Ting Xu,Hao-Xian Zhu,Xiao-Min Chen,Liang Li,Zhi-Bin Zhao,Liwei Lu,Yang Li,Zhe-Xiong Lian,Cai-Yue Gao
Objective Activated T cells play a pivotal pathogenic role in the progression of Sjögren disease (SjD); however, there are currently no targeted therapies specifically designed to address them. This study aims to identify pathogenic CD8 + T cells in SjD and develop targeted therapeutic strategies. Methods Il12b-/-Il2ra-/- mice, a murine model for overlapping primary biliary cholangitis and SjD, were employed in this study. Pathogenic CD8 + T cells were identified through single-cell RNA sequencing and flow cytometry analyses of samples from both patients with SjD and relevant murine models. Shared T cell receptor analysis was conducted to trace the potential precursors of pathogenic CD8 + T cells. The efficacy of PD-1-targeted chimeric antigen receptor (CAR)–T cell therapy was evaluated through the assessment of salivary gland secretory function, immunologic profiles, and histopathological changes in the murine model. Results We identified PD-1 as a comprehensive marker of clonally expanded and activated pathogenic CD8 + T cells in the salivary glands and peripheral tissues. Flow cytometry further confirmed the activation phenotype and cytotoxicity of PD-1 + CD8 + T cells in the salivary glands of patients with SjD. Notably, the number of PD-1 + CD8 + T cells in the labial glands positively correlated with disease activity in patients with SjD. These findings highlight the therapeutic potential of depleting PD-1 + CD8 + T cells in SjD. Furthermore, PD-1-targeted CAR-T cell therapy significantly alleviated SjD symptoms in a murine model. Conclusion We identified the pathogenic role of PD-1 + CD8 + T cells in both patients with SjD and a murine model and demonstrated the efficacy of PD-1-targeted CAR-T cell therapy in SjD model mice. Our findings suggest a promising avenue for developing clinical therapeutic strategies for patients with SjD.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）