ACS Central Science
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2024
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IF 12.7
Yujie Wang,Zhengao Di,Minglang Qin,Shenming Qu,Wenbo Zhong,Lingfeng Yuan,Jing Zhang,Julian M. Hibberd,Ziyi Yu
In this study, an innovative approach is presented in the field of engineered plant living materials (EPLMs), leveraging a sophisticated interplay between synthetic biology and engineering. We detail a 3D bioprinting technique for the precise spatial patterning and genetic transformation of the tobacco BY-2 cell line within custom-engineered granular hydrogel scaffolds. Our methodology involves the integration of biocompatible hydrogel microparticles (HMPs) primed for 3D bioprinting with Agrobacterium tumefaciens capable of plant cell transfection, serving as the backbone for the simultaneous growth and transformation of tobacco BY-2 cells. This system facilitates the concurrent growth and genetic modification of tobacco BY-2 cells within our specially designed scaffolds. These scaffolds enable the cells to develop into predefined patterns while remaining conducive to the uptake of exogenous DNA. We showcase the versatility of this technology by fabricating EPLMs with unique structural and functional properties, exemplified by EPLMs exhibiting distinct pigmentation patterns. These patterns are achieved through the integration of the betalain biosynthetic pathway into tobacco BY-2 cells. Overall, our study represents a groundbreaking shift in the convergence of materials science and plant synthetic biology, offering promising avenues for the evolution of sustainable, adaptive, and responsive living material systems.
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高影响因子
Small
·
2025
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IF 12.1
Shaojian Jiang,Yuhang Liu,Ruidong Yang,Lijun Zhang,Wenke Liu,Kai Deng,Hongjie Yu,Hongjing Wang,Liang Wang
Since formaldehyde oxidation reaction (FOR) can release H 2 , it is attractive to construct a bipolar hydrogen production system consisting of FOR and hydrogen evolution reaction (HER). Although copper-based catalysts have attracted much attention due to their low cost and high FOR activity, the performance enhancement mechanism lacks in-depth investigation. Here, an amorphous-crystalline catalyst of amorphous nickel hydroxide-coated copper dendrites on copper foam (Cu@Ni(OH) 2 /CF) is prepared. The modification of Ni(OH) 2 resulted in hydrophilic and aerophobic states on the Cu@Ni(OH) 2 /CF surface, facilitating the transport of liquid-phase species on the electrode surface and accelerating the release of H 2 . The Open circuit potential (OCP) and density functional theory (DFT) calculations indicate that this core–shell structure facilitates the adsorption of HCHO and OH − . In addition, the catalytic mechanism and reaction pathway of FOR are investigated through in situ FTIR and DFT calculations, and the results showed that the modification of Ni(OH) 2 lowered the energy barrier for C─H bond breaking and H─H bond formation. In the HER//FOR system, Pt/C//Cu@Ni(OH) 2 /CF can provide a current density of 0.5 A cm −2 at 0.36 V and achieve efficient and stable H 2 production. This work offers new ideas for designing electrocatalysts for bipolar hydrogen production system assisted with formaldehyde oxidation.
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PHYTOMEDICINE
·
2025
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IF 8.3
Feng Jia,Yongqing Wang,Zhiqiang Chen,Jingxian Jin,Lei Zeng,Li Zhang,Huaijian Tang,Yanyan Wang,Pei Fan
Background 10-Hydroxydec-2-enoic acid (10-HDA), a unique and marker compound in royal jelly, has a wide range of bio-activities. However, its role in regulating inflammation of vascular smooth muscle cell (VSMC), which is essential to a set of vascular diseases, is still unknown. Purpose Our study aimed to investigate whether 10-HDA exerts effect on VSMC inflammation via interacting with toll-like receptor 4 (TLR4), a pivotal inflammatory initiator. Methods A package of proteins, which might participate in TLR4-mediated signaling, influenced by 10-HDA were analyzed in mouse VSMCs with Angiotensin Ⅱ(Ang Ⅱ) or lipopolysaccharide (LPS) stimulation. Accordingly, pro- or anti-inflammatory cytokines, reactive oxygen species (ROS), and anti-oxidants that are closely relevant to inflammatory process were determined. The possible mode for 10-HDA interacting with TLR4 was also characterized. Moreover, involvement of a key miRNA in 10-HDA regulating VSMC inflammation was identified. Results In the presence of Ang Ⅱ, 10-HDA inhibited the TLR4 expression in a dose-dependent manner. In such occasion, 10-HDA hindered the up-regulation of specificity protein 1 (SP1) and serine/threonine-protein phosphatase 6 catalytic subunit (PPP6C), the phosphorylation of extracellular signal-regulated kinase 1/2, TGF-β-activated kinase 1, and nuclear factor-κB p56, as well as the enhancement of myeloid differentiation primary response gene 88. Apart from SP1 and PPP6C, the level change of these proteins by 10-HDA was similar with LPS stimulation. The effect might be resulted from 10-HDA blocking TLR4 through multiple atomic interactions. 10-HDA mitigated the increase of pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin-2 (IL-2), and IL-6, as well as increased the anti-inflammatory cytokine IL-10, in the Ang Ⅱ- or LPS-induced VSMCs. Correspondingly, the level of ROS was attenuated and the anti-oxidants such as glutathione and superoxide dismutase were fortified. The data indicated the anti-inflammatory potential of 10-HDA in VSMCs, which was associated with 10-HDA's capability of relieving oxidative stress. Additionally, the expression of miR-17–5p was saved by 10-HDA from Ang Ⅱ- or LPS-treated VSMCs, which might be relevant to SP1 and PPP6C targeting. Conclusion The present work of 10-HDA, for the first time, revealed its ability to alleviate VSMC inflammation by targeting TLR4 and therefore modulate the downstream inflammatory participants. Our data will cast light on the utilization of 10-HDA in VSMC inflammation-related vascular disorders.
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Science China-Chemistry
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2010
·Vol. 53
·期 7
·1497-1501
·
IF 0
Ma Hong,Nie Xin,Cai JiaYing,Chen Chen,Gao Jin,Miao Hong,Xu Jie
The catalytic oxidation of biomass-derived polyhydroxyl compounds provides an efficient and green route for biomass resource utility. Gold nanocrystals supported on Mg(OH) 2 were synthesized by sol-immobilization method, characterized by XRD, TEM, UV-vis DRS, and tested in the selective oxidation of 1,2-propanediol to lactic acid. The gold particle size and the ratio of Au to 1,2-propanediol strongly influenced the catalytic activity. Over Au/Mg(OH) 2 with the majority of gold particles concentrated in 14–18 nm, excellent catalytic performance with 94.4% conversion and 89.3% selectivity to lactic acid has been achieved under 0.3 MPa O 2 at 60 °C for 6 h.
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Food Bioscience
·
2024
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IF 4.8
Jiajing Liao,Jin Qian,Lijuan Rao,Suqin Lin,Chen Wang,Linqian Xu,Bing Yuan,Jiayi Yuan,Yin Wan,Guiming Fu
Hyperuricemia is a metabolic disease caused by excessive production of uric acid or abnormal excretion of uric acid. Although most of the uric acid is excreted in the kidneys, the elimination of uric acid in the intestines still plays an important role. 3,5-dicaffeoylquinic acid (3,5-diCQA) is widely found in Chrysanthemum and Artemisia species, where it inhibits the activity of xanthine oxidase (XOD), a key enzyme in uric acid production. Therefore, a Caco-2 cell model was utilized to explore the mechanisms of 3,5-diCQA affecting uric acid excretion under high uric acid conditions, focusing on aspects such as intestinal uric acid transport, the intestinal barrier, and the MAPK signaling pathway. The results showed that 3,5-diCQA could reduce the oxidative stress and apoptosis induced by high uric acid, repair the intestinal barrier, upregulate the expression of ABCG2 and MRP4, promote the excretion of uric acid and inhibit the activation of MAPK signaling pathway. In addition, 3,5-diCQA could also regulate the expression of ABCG2 and MRP4 via the MAPK signaling pathway. These findings provide scientific evidence for dietary recommendations for individuals with hyperuricemia, suggesting that foods or supplements containing 3,5-diCQA may help manage uric acid levels.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Kegang Wu,Tong Zhang,Xianghua Chai,Pingping Wang,Xuejuan Duan,Huiying Hu
This study investigated the antibacterial activity and mechanism of action of 2-methoxycinnamaldehyde (MCA) against Salmonella enteritidis and evaluated the efficacy of MCA as a food disinfectant. The minimum inhibitory concentration (MIC) of MCA against S. enteritidis was 0.16 mg/mL. The acquired antimicrobial resistance toward MCA was weak, with low hemolytic activity and good in vitro stability. S. enteritidis exposed to MCA exhibited the formation of both holes and grooves on the cell surface, alterations in the structure and conformation of membrane proteins, leakage of nucleic acids, production of reactive oxygen species, membrane lipid peroxidation, binding to the groove of DNA, and destruction of protein secondary structures. After treatments with 1 MIC, 2 MIC, and 4 MIC of MCA for 20 min, S. enteritidis strain in pork slices was significantly inhibited ( p < 0.05), and the reduction amounts ranged from 1.31 to 4.98 log CFU/g. Additionally, the treated pork contained low levels of residual disinfectants and demonstrated high sensory acceptability. This study provides new insights into the antibacterial mechanism of MCA, which has broad application potential as a plant-derived antibacterial washing disinfectant to prevent foodborne pathogens.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
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IF 8.1
Boliang Li,Binfei Li,Liping Du,Teng Lu,Guolin Shao,Zhaomin Li,Mengyuan Zhang,Junhao Zhang
High carbon emissions and low efficiency in thermal utilization are pivotal challenges in heavy oil development. Addressing the urgent need to enhance heavy oil production, this paper introduces an innovative approach in which aerogel and flue gas serve as supplementary additives in steam thermal recovery. To investigate the mechanisms behind the improved heat transfer and oil recovery facilitated by the combination of aerogel and flue gas, condensation heat transfer experiments and 2-D visual oil displacement experiments were carried out. The condensation heat transfer coefficient, oil displacement performance, and heat utilization efficiency were analyzed and compared. The results showed the porous structure of the aerogel enhances its thermal insulation properties by adsorbing flue gas, thereby reducing the steam-to-cold object condensation heat transfer coefficient by 60.6%. The cementation between aerogel nanoparticles (NPs) retained in the formation and rock particles formed an insulating layer that impedes the upward propagation of steam heat. Combined with efficacy of flue gas foam in improving fluid flow, this promoted the horizontal expansion of the steam chamber, resulting in an 8.2% and 3.4% increase in the steam chamber’s extent compared to using steam and a combination of steam with aerogels, respectively. Moreover, the profile control and thermal insulation impacted following the NPs, lowering the water cut in the produced fluid and enhancing the oil recovery by 7.9%. Upon the application of flue gas, this enhancement in recovery extended to 9.3%.
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LANGMUIR
·
2025
·
IF 3.9
Xihong Ding,Demeng Qian,Hui Wang,Qian Hu,Lin Zhu,Hui Chen,Tengda Xu,Aohan Li,Wei Hong
This study investigates the role of 2-aminoethanethiol hydroiodate (2-AET·HI) additives in enhancing the phase stability and photovoltaic performance of CsPbI3 films for inorganic perovskite solar cells. The incorporation of 2-AET·HI into the precursor solution results in a more uniform and compact film morphology. X-ray diffraction (XRD) analysis reveals that the 2-AET·HI-treated CsPbI3 films retain their black α-phase, even after 300 h at 25% RH and ∼25 °C, whereas the control samples show a rapid transition to the nonphotoactive δ-phase. This improvement in phase stability is reflected in the enhanced photovoltaic performance, with the 2-AET·HI-modified devices exhibiting a power conversion efficiency (PCE) of 16.7%, compared to 15.4% for the control devices. Furthermore, the 2-AET·HI-modified devices display superior long-term stability, retaining 83% of their initial PCE after 192 h in ambient conditions, in stark contrast to the unmodified devices, which degrade rapidly.
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JOURNAL OF PLANT PATHOLOGY
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2024
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IF 2.2
Reymick Okwong Oketch,Duan Bin,Feng Zhao,Zhang Yong Hua,Ouyang Qiuli,Kaaya Archileo N.,Tao Nengguo
Management of Penicillium digitatum requires use of highly effective fungicides that are not prone to drug resistance and are safe to humans and the environment. This study examined the fungicidal potential of combinations of cuminaldehyde (CA) + trans-2-hexen-1-al (TH) and CA + Citral. Both CA + TH and CA + Citral exhibited enhanced fungitoxicity against P. digitatum than CA, citral or TH alone. The minimum inhibitory concentration (MIC) of CA + Citral consisted of 0.13 ml/L CA and 0.3 ml/L citral, representing a synergistic interaction. MIC of CA + TH consisted of 0.12 ml/L CA and 0.15 ml/L TH, representing an additive interaction. The minimum fungicidal concentration (MFC) of CA + Citral and of CA + TH consisted of 0.26 ml/L CA and 0.6 ml/L citral, and 0.18 ml/L CA and 0.24 ml/L TH, respectively. 10×MFC CA + Citral in Tween-80 (WCI) delayed onset of hyphal growth in fruits inoculated with P. digitatum to day 5 compared to day 4 in the 10×MFC CA + TH in Tween-80 (WCT) treatment, and day 3 for the control samples. Daily green mold disease incidence and disease severity were much lower in WCI and WCT treatments than in the 10×MFC CA in Tween-80 (WCA) treatment and in control samples. Our results show that WCI was much fungitoxic against P. digitatum than WCT and WCA, its effect being comparable to that of imazalil. Additionally, at 25 ± 2 °C and 85–90% relative humidity, both WCI and WCT markedly preserved fruit quality by day 6 of treatment. WCI and WCT are thus, safe, cheap and highly effective alternatives to chemical fungicides being used to control postharvest green mold in citrus fruits.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
·
IF 5.2
Zixuan Wang,Yingxiao Sun,Peijie Dong,Jinhu Wang,Lijie Wang,Aili Zhao,Guangmin Qu,Hang Li,Kannapathirage Dilini Maheshika Gunarathne,Wen Zhang,Yao Chen,Xin Meng
Trauma healing is the process of healing after the body has been subjected to an external force and the skin and other tissues have become dissected or defective, showing the synergistic effect of various processes. Therefore, the investigation of innovative wound dressings has significant research and clinical implications. In this study, we constructed a zinc based metal–organic framework (MOF) and loaded with antimicrobial peptide LL37 to prepare LL37@ZPF-2 (ZPF = zeolite pyrimidine backbone), which was subsequently integrated with Poloxamer 407 to fabricate LL37@ZPF-2 thermosensitive hydrogel. Our study showed that in-situ packaging method can achieve encapsulation rate of 98 % and 15 % of drug loading for LL37. LL37@ZPF-2 demonstrated a higher inhibitory potency against S.aureus compared to E.coli . The Poloxamer 407-gel exhibits thermo-responsive sol–to–gel phase transition behaviors with a phase transition temperature (Tsol/gel) of ∼ 28.01℃, making it an appropriate material for wound healing. The composite hydrogel has excellent biocompatibility and hemocompatibility. A full-thickness skin defect model was built to confirm that LL37@ZPF-2 thermosensitive hydrogel dressing could inhibit bacterial growth, reduce the risk of wound infection, and stimulate angiogenesis and collagen deposition, resulting in a wound healing rate of 94.4 % on day 7 and complete healing on day 10. Our findings demonstrate that the novel thermosensitive LL37@ZPF-2 hydrogel confers good antibacterial activity, promoting cell migration and infected-wound healing properties, providing a promising platform for wound healing.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Peng-Fei Qiu,Xiu-Niang Tan,Zai-Yin Huang,Yan Zhou
The low conductivity of many traditional metal–organic-framework (MOF)-based electrode limits their developments in the field of electrochemical energy storage and still of great challenge. The controllable preparation of various kinds of nanomaterials using thiol-functionalized MOF shows great prospects. In this work, a thiol-functionalized metal–organic framework sheet structure ( Co-MOF/NF ) on nickel foam was successfully prepared by in situ interfacial growth synthesis, which was transformed into its derivatives S-doped β-Co(OH) 2 nanoflowers Co-x/NF ( x = 1, 2 and 6) in different concentrations of KOH solutions through ion etching/exchange reaction. The pristine thiol-functionalized Co-MOF/NF and its derivatives Co-x/NF ( x = 1, 2 and 6) nanoflower-like arrays could be used as positive electrode materials for effective supercapacitors. Among them, the transformation of the nanoflower-like Co-1/NF electrode exhibits excellent electrochemical properties with high areal capacitance (1925 ± 23 mF/cm 2 at 1 mA/cm 2 ), good rate performance, excellent conductivity and decent cycling stability. The Co-1//AC ASC device provides a high energy density of 0.176 mWh/cm 2 (92.6 Wh/kg) at a power density of 0.745 mW/cm 2 (392.1 W/kg). And this Co-1//AC ASC device exhibits a good cycling stability and practical application in energy storage field. This study provides a new strategy for the pristine thiol-functionalized MOF and its conversion nanostructures for energy storage applications.
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高影响因子
Biomarker Research
·
2024
·Vol. 12
·期 1
·1-16
·
IF 9.5
Wang Zhen,Liu Zhongwen,Zhou Pan,Niu Xiaona,Sun Zhengdao,He Huan,Zhu Zunmin
Background Megakaryocytic differentiation is a complicated process regulated by a series of transcription factors in a context- and stage-dependent manner. Recent studies have suggested that krüppel-like transcription factor 2 (KLF2) is involved in the control of embryonic erythroid precursor cell differentiation and maturation. However, the function and mechanism of KLF2 in regulating megakaryocytic differentiation remain unclear. Methods The expression patterns of krüppel-like transcription factors (KLFs) during megakaryocytic differentiation were identified from public databases. Phorbol 12-myristate 13-acetate (PMA) treatment of the myeloid-erythroid-leukemic cell lines K562 and HEL were used as cellular megakaryocytic differentiation models. A lentiviral transduction system was utilized to achieve the goal of amplifying or reducing KLF2. The expression of KLF2 was examined using real-time PCR and western blot. The impact of KLF2 on the megakaryocytic differentiation of K562 cells was examined by flow cytometry, Giemsa staining, Phalloidin staining and western blot. RNA-sequencing (RNA-seq) and chromatin immunoprecipitation-sequencing (ChIP-seq) technologies were used to identify the KLF2-regulated targets. Results KLF2 is increased in the maturation process of megakaryocytes. KLF2 overexpression accelerated the PMA-induced megakaryocytic differentiation, as reflected by an increased percentage of CD41/CD61 cells, an increased number of polyploid cells, and an elevated expression of P21 and P27. KLF2 knockdown exhibited the opposite results, indicating that KLF2 knockdown suppressed the megakaryocytic differentiation. Further, combination of the RNA-seq and ChIP-seq results suggested that chimerin 1 (CHN1) and potassium voltage-gated channel subfamily Q member 5 (KCNQ5) may be target genes regulated of KLF2. Both CHN1 and KCNQ5 knockdown could block the megakaryocytic differentiation to some content. Conclusion This study implicated a regulatory role of KLF2 in megakaryocytic differentiation, which may suggest KLF2 as a target for illness with abnormal megakaryocytic differentiation.
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