ANALYTICAL SCIENCES
·
2023
·Vol. 39
·期 10
·1669-1679
·
IF 1.6
Lin Xiaorong,Wang Changting,You Liuxia,Fu Fanrui,Liu Qiaoling
In this work, PtCo bimetallic nanoparticles supported on multi-walled carbon nanotubes (PtCo@MWCNTs) nanohybrid was prepared simply and used for the first time as a novel nanozyme in the colorimetric sensing of L-cysteine (L-Cys) and Cu 2+ . Due to its strong enzyme-like catalytic activity, the prepared PtCo@MWCNTs nanohybrid can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to form ox-TMB without H 2 O 2 . Interestingly, the oxidase-like active of PtCo@MWCNTs was effectively suppressed by L-Cys, which could reduce ox-TMB to colorless TMB and lead to a pronounced blue fading, and the absorbance at 652 nm gradually decreased with increasing L-Cys concentration. On the other hand, the nanozyme activity of PtCo@MWCNTs can be recovered due to the complexation between L-Cys and Cu 2+ . Therefore, a colorimetric method based on PtCo@MWCNTs nanozyme was established to detect L-Cys and Cu 2+ . The results show that the assay platform has simple, rapid, sensitive performance and good selectivity. The detection limits for L-Cys and Cu 2+ are 0.041 μM and 0.056 μM, respectively, coupled with the linearities of 0.01 ~ 60.0 μM and 0.05 ~ 80.0 μM. The successful first application of PtCo bimetal-based nanozyme in colorimetric sensing herein opens a new direction for nanozyme and colorimetric analysis, showing great potential applications. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 7
·1-10
·
IF 5.7
Chen Tingting,Qin Yingfeng,Wang Beibei,Lai Rongji,Tan Guohe,Liu Jin-Wen
A novel fluorescent strategy has been developed by using an enzymatic reaction modulated DNA assembly on graphitic carbon nitride nanosheets (CNNS) for the detection of acetylcholinesterase (AChE) activity and its inhibitors. The two-dimensional and ultrathin-layer CNNS-material was successfully synthesized through a chemical oxidation and ultrasound exfoliation method. Because of its excellent adsorption selectivity to ssDNA over dsDNA and superior quenching ability toward the fluorophore labels, CNNS were employed to construct a sensitive fluorescence sensing platform for the detection of AChE activity and inhibition. The detection was based on enzymatic reaction modulated DNA assembly on CNNS, which involved the specific AChE-catalyzed reaction-mediated DNA/Hg 2+ conformational change and subsequent signal transduction and amplification via hybridization chain reaction (HCR). Under the excitation at 485 nm, the fluorescence signal from 500 to 650 nm ( λ max = 518 nm) of the developed sensing system was gradually increased with increasing concentration of AChE. The quantitative determination range of AChE is from 0.02 to 1 mU/mL and the detection limit was 0.006 mU/mL. The developed strategy was successfully applied to the assay of AChE in human serum samples, and can also be used to effectively screen AChE inhibitors, showing great promise providing a robust and effective platform for AChE-related diagnosis, drug screening, and therapy. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RARE METALS
·
2023
·Vol. 42
·期 10
·3443-3454
·
IF 8.8
Zhou Ming-Jie,Zhang Wen-Tao,Li Zhun,Feng Tao,Lan Si,Peng Zhen,Chen Shuang-Qin
Metallic glasses (MGs) are promising heterogeneous catalysts in water remediation, due to their superior efficiency, selectivity, reusability and corrosion resistance. However, few works are focused on the influence of inorganic anions that are abundant in wastewater. Herein, four common inorganic anions were added in a heterogeneous Fenton-like system (Fe–MG/H 2 O 2 ) to study inorganic anions’ influence on MGs’ catalytic performance during methylene blue (MB) degradation. Evidence demonstrated that chloride ions and dihydrogen phosphate ions had an adverse effect on the catalytic performance of Fe–MG, whereas Fe–MG/H 2 O 2 system sustained high efficiency in the presence of sulfate ions and nitrate ions during the Fenton-like process. By studying the structure, surface morphology, and evolution of active species, it was found that inorganic anions had a significant effect on the surface morphology of Fe–MG and the generation of active species. This work will provide essential references for MGs as heterogeneous catalysts in practical applications. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-12
·
IF 3.125
Zhang Xueyan,Wu Fengbo,Men Ke,Huang Rong,Zhou Bailin,Zhang Rui,Zou Rui,Yang Li
As a novel toll-like receptor 9 (TLR9) agonist, synthetic unmethylated cytosine-phosphate-guanine (CpG) oligodeoxynucleotides can stimulate a Th1 immune response and potentially be used as therapeutic agents or vaccine adjuvants for the treatment of cancer. However, some drawbacks of CpG limit their applications, such as rapid elimination by nuclease-mediated degradation and poor cellular uptake. Therefore, repeat high-dose drug administration is required for treatment. In this work, a CpG delivery system based on 3-aminopropyltriethoxysilane (APTES)-modified Fe 3 O 4 nanoparticles (FeNPs) was designed and studied for the first time to achieve better bioactivity of CpG. In our results, we designed FeNP-delivered CpG particles (FeNP/CpG) with a small average size of approximately 50 nm by loading CpG into FeNPs. The FeNP/CpG particle delivery system, with enhanced cell uptake of CpG in bone marrow-derived dendritic cells (BMDCs) in vitro and through intratumoral injection, showed significant antitumor ability by stimulating better humoral and cellular immune responses in C26 colon cancer and 4T1 breast cancer xenograft models in vivo over those of free CpG. Moreover, mice treated by FeNP/CpG particles had delayed tumor growth with an inhibitory rate as high as 94.4%. In addition, approximately 50% of the tumors in the C26 model appeared to regress completely. Similarly, there were lower pulmonary metastases and a 69% tumor inhibitory rate in the 4T1 breast cancer tumor model than those in the untreated controls. In addition to their effectiveness, the easy preparation, safety, and high stability of FeNP/CpG particles also make them an attractive antitumor immunotherapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 14
·3325-3335
·
IF 3.307
Liu Yue,Lu Shan,Zhao Liang,Dong Xin,Zhu Zhenyu,Jin Yongsheng,Chen Haisheng,Lu Feng,Hong Zhanying,Chai Yifeng
Studies have documented the potential antitumor activities of glaucocalyxin A (GLA), an ent-kaurene diterpenoid isolated from Rabdosia japonica . However, the metabolic mechanism underlying the antitumor activity of GLA remains largely unknown. The effects of GLA on the metabolome of human liver cancer cells using GC/MS- and LC/MS-based metabolic profiling have been investigated. An untargeted metabolomics approach in conjunction with orthogonal projection to latent structures–discriminant analysis (OPLS-DA) has been developed to characterize the metabolic modifications induced by GLA treatment in human hepatoma cell line SMMC7721. Results demonstrated that cells cultured in the presence or absence of GLA displayed different metabolic profiles: the treatment induced an increased purine metabolism, pyrimidine metabolism, and sphingolipid metabolism and a decreased amino acid metabolism. At the same time, GLA treatment induced cell apoptosis and cell cycle arrested at G2/M phase in a dose-dependent manner. In addition, two representative apoptosis-inducing cytotoxic agents were selected as positive control drugs to validate the reasonableness and accuracy of our metabolomic investigation on GLA. The study displayed a systemic metabolic alteration induced by GLA treatment, showing the impaired physiological activity of SMMC7721 cells, which also indicated anti-proliferative and apoptotic effects of GLA. In the meantime, GC/MS- and LC/MS-based metabolomics applied to cell culture enhanced our current understanding of the metabolic response to GLA treatment and its mechanism; such an approach could be transferred to study the mechanism of other anticancer drugs.
A systemic metabolic alteration induced by glaucocalyxin A (GLA) treatment of SMMC-7721 cells
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Communication and Signaling
·
2026
·
IF 11.6
Liu Fang,Han Chao,Xiao Yi
Histone lactylation is a recently identified epigenetic modification that directly couples cellular metabolic state with gene expression. Whether lactate affects host resistance to pathogens via histone lactylation remains unclear. Here, we found that infection with Pseudomonas aeruginosa PA14 significantly increased lactate levels in Caenorhabditis elegans . Exogenous sodium lactate (NAL) also raised lactate levels and reduced worm resistance to the extracellular bacteria P. aeruginosa and Enterococcus faecalis , but had no detectable effect on the intracellular bacteria Salmonella and Listeria monocytogenes . Lowering lactate levels with the lactate dehydrogenase inhibitor Oxamate enhanced worm resistance to infection. Genetic screening showed that the regulatory effects of NAL and Oxamate on immunity were dependent on SKN-1. Similar results were also observed with pyruvate and the pyruvate dehydrogenase kinase inhibitor dichloroacetate (DCA) treatment. Mechanistic studies showed that, among the histone lactylation sites examined, elevated lactate promoted lactylation most strongly at H4K16 (H4K16la). ChIP‑qPCR results showed increased H4K16la enrichment at the skn-1 promoter upon lactate elevation. This led to increased activity of the transcription factor SKN-1, as evidenced by increased SKN-1 nuclear translocation, upregulation of downstream genes ( skn-1 , gst-4 , gst-7 , gst-10 , gcs-1 ), and enhanced GST-4::GFP fluorescence. However, the addition of NAL caused excessive GST-4::GFP fluorescence, indicating SKN-1 overactivation. This also led to suppression of the antimicrobial peptide IRG-1 and increased bacterial accumulation in the gut. In summary, this study provides evidence that lactate suppresses innate immunity in C. elegans , likely through H4K16 lactylation-mediated overactivation of SKN-1. These findings suggest that lactate may act as a negative regulator of innate immunity in an invertebrate model and provide a basis for future studies on targeting lactate metabolism or histone lactylation in infectious diseases.
✓ 已验证
产品关联
DOI
高影响因子
Chinese Journal of Integrative Medicine
·
2026
·
IF 3.1
Zhang Di,Zhuang Zi-feng,Lin Shao-zi,Chen Zen-ni,Chen Hong-ling,Gu Jun-yan,Hao Yu-ting,Li Yi-xuan,Chen Jia-xu,Zhao Guo-ping
Objective To elucidate whether Danggui Sini Decoction (DGSD) relieves sciatica by downregulating transient receptor potential (TRP) channels involved in peripheral sensitization. Methods After conducting chronic constriction injury of sciatic nerve (CCI) operation, 32 SD rats, randomly assigned to the sham, CCI 7d, CCI 14d, and CCI 21d groups ( n =8 per group), were used to observe the course of sciatica. mRNA sequencing of dorsal root ganglia (DRG) was conducted on rats in the sham and CCI 21d groups. Another 32 rats were divided into the sham, CCI, mecobalamin, and DGSD groups, 8 in each group. mRNA sequencing analysis and single-cell RNA-sequencing (scRNA-seq) data analysis were conducted. Behavioural tests were conducted to evaluate the therapeutic effects of DGSD. The levels of TRP vanilloid (TRPV)1–4, TRP ankyrin 1 (TRPA1), TRP melastatin 8 (TRPM8), Ras homolog family member A (RhoA), and phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK) were measured via Western blot, and the serum levels of C-C motif chemokine ligand 2 and 5 (CCL2, CCL5), substance P (SP), and prostaglandin E2 (PGE2) were conducted via ELISA. Results scRNA-seq and mRNA sequencing analysis revealed that inflammatory mediator regulation of TRP channels played a role in the development of sciatica. CCI rats exhibited cold hyperalgesia from day 4 to 21, increased the DRG protein expression levels of TRP channels, including TRPV1–4, TRPA1, TRPM8, RhoA, p-p38MAPK, CCL2, CCL5, SP, and PGE2. Treatment with DGSD decreased the serum levels of CCL2, CCL5, SP, and PGE2, and reduced the levels of RhoA, p-p38MAPK, TRPV1–4, TRPA1, and TRPM8 in DRG ( P <0.05). Conclusion CCI increased the levels of TRP channels and inflammatory factors, inducing cold hyperalgesia, whereas DGSD relieved pain via inhibiting peripheral sensitization to relieve cold hyperalgesia by reducing the inflammatory factors and TRP.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·
IF 4.3
Hu Xingru,Pang Long,Wu Mingkai,Wang Chaohai,Li Jiansheng
Metal–organic framework-derived carbon materials have shown extensive application in the sensitive extraction of polycyclic aromatic hydrocarbons (PAHs), but more active sites for its adsorption were still a tireless pursuit. In this study, ZIF-nanoleaf-derived carbon (NLCs) was synthesized and developed as a solid-phase microextraction (SPME) fiber (NLCs-F). The extraction performance was compared with ZIF-dodecahedron-derived carbon (DHCs) coated fiber (DHCs-F), which was prepared by only changing the ratio of the reactants. The unique morphology of NLCs provided abundant adsorption active sites for the selected PAHs, while the large average aperture facilitated selective extraction of high molecular weight analytes. Additionally, the high carbon content enhanced the strong enrichment capability for hydrophobic PAHs. Hence, the prepared NLCs-F coupled with GC–MS showed a good correlation coefficient (0.9975) in a wide linear range, low limits of detection (0.3–1.8 ng L −1 ), satisfactory repeatability, and reproducibility, which made it apply in the enrichment of PAHs in actual tea and coffee samples. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-13
·
IF 16.6
Wang Xingkun,Xu Liangliang,Li Cheng,Zhang Canhui,Yao Hanxu,Xu Ren,Cui Peixin,Zheng Xusheng,Gu Meng,Lee Jinwoo,Jiang Heqing,Huang Minghua
Dual atom catalysts, bridging single atom and metal/alloy nanoparticle catalysts, offer more opportunities to enhance the kinetics and multifunctional performance of oxygen reduction/evolution and hydrogen evolution reactions. However, the rational design of efficient multifunctional dual atom catalysts remains a blind area and is challenging. In this study, we achieved controllable regulation from Co nanoparticles to CoN 4 single atoms to Co 2 N 5 dual atoms using an atomization and sintering strategy via an N-stripping and thermal-migrating process. More importantly, this strategy could be extended to the fabrication of 22 distinct dual atom catalysts. In particular, the Co 2 N 5 dual atom with tailored spin states could achieve ideally balanced adsorption/desorption of intermediates, thus realizing superior multifunctional activity. In addition, it endows Zn-air batteries with long-term stability for 800 h, allows water splitting to continuously operate for 1000 h, and can enable solar-powered water splitting systems with uninterrupted large-scale hydrogen production throughout day and night. This universal and scalable strategy provides opportunities for the controlled design of efficient multifunctional dual atom catalysts in energy conversion technologies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RARE METALS
·
2023
·Vol. 42
·期 12
·4015-4028
·
IF 8.8
Ma Xiao-Fang,Xiao He,Jing Yan-Ying,Gao Yang,He Ying-Luo,Zhao Man,Jia Jian-Feng,Wu Hai-Shun
Ru-based electrocatalysts have demonstrated excellent electrocatalytic performance toward hydrogen evolution reaction (HER). However, designing Ru nanoparticles/nanoclusters (Ru NPs/NCs) with abundant accessible active sites is challenging. Herein, a selective facile hydrothermal in situ polymerization strategy is described to accurately control the anchoring point of Ru in hollow mesoporous carbon spheres (HMCs) (outer surface, HMCs@Ru; cavity, Ru@HMCs; and pore channels, Ru/S-HMCs). The HER mechanisms of the three catalysts over a wide pH range were revealed by analyzing the loading position, content, dispersion, particle size, and valence state of Ru. HMCs@Ru shows excellent area activity, with overpotentials of 64.3, 2.8, and 78.1 mV at 10 mA·cm −2 in 0.5 mol⋅L −1 H 2 SO 4 , 1 mol⋅L −1 KOH, and 0.5 mol⋅L −1 phosphate buffer saline (PBS) solutions, respectively. Ru/S-HMCs exhibit high mass activity, with values 43.3, 2.9, and 12.7 times those of Pt/C in alkaline, acidic, and neutral media, respectively, at − 50 mV (vs. reversible hydrogen electrode (RHE)). After 2000 cyclic voltammetry cycles, the performances of HMCs@Ru and Ru/S-HMCs hardly decrease, indicating good stability. The excellent electrocatalytic activity can be attributed to two main factors: (1) the confinement effect, which not only promotes high Ru dispersion but also prevents the leaching of active metal under harsh conditions, and (2) their excellent structural characteristics. The catalyst structure features a large hollow located in the center (~ 160 nm in diameter) and an ultrathin monolayer of mesopores (~ 6 nm) uniformly distributed on the hollow shells, ensuring fast mass diffusion and electron transfer kinetics, as well as complete accessibility of catalytic sites. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
STRAHLENTHERAPIE UND ONKOLOGIE
·
2023
·
IF 3.1
Huang Hui,Xue Jie,Xie Mei-Lin,Xie Tao
Purpose Osthole possesses anti-tumor activities. However, whether osthole can have a radiosensitization effect on hepatic cancer remains unclear. Here, an HCC-LM3 cells-inoculated subcutaneous transplanted tumor was adopted to explore the effect of osthole. Methods The tumor-bearing mice were treated with 100 mg/kg osthole for 12 days, 4 Gy irradiation twice, or their combination. The tumor volume and weight, lactic acid content, glycolytic enzyme activities, and protein expression of glycogen synthase kinase 3β (GSK-3β), p‑GSK-3β, mammalian target of rapamycin (mTOR), p‑mTOR, AMP-activated protein kinase (AMPK), p‑AMPK, glucose transporter 1/3, and pyruvate kinase M2 were determined. The GSK-3β-overexpressed HCC-LM3 or SK-Hep‑1 cell models were also adopted to verify the effects of osthole on expression of these proteins. Results The tumor volume and weight, lactic acid content, and glycolytic enzyme activities in tumor tissues were lower in the osthole + radiation group than in the radiation group. Moreover, osthole could reverse the radiation-induced increments of p‑GSK-3β/GSK-3β and p‑mTOR/mTOR protein ratios and the expression of glucose transporter 1/3 and pyruvate kinase M2 proteins in tumor tissues, and increase the protein ratio of p‑AMPK/AMPK. The effects of osthole on these glycolysis-related proteins were also observed in GSK-3β-overexpressed HCC-LM3 or SK-Hep‑1 cell models. Conclusion Osthole has a radiosensitizing effect on subcutaneous transplanted hepatocellular carcinoma, and its mechanism may be related to inhibition of GSK-3β/AMPK/mTOR pathway-controlled glycolysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Fiber Materials
·
2023
·
IF 16.1
Cui Yifan,He Xinyang,Liu Wendi,Zhu Suiyuan,Zhou Man,Wang Qiang
Stretchable thermoelectric-based self-powered sensors have attracted widespread attention for wearable electronic devices. However, the development of thermoelectric materials with wearable comfort, green, and multimodal synergy remains challenging. In this paper, we prepared a poly(3,4-ethylenedioxythiophene)/multi-walled carbon nanotube (PEDOT/MWCNT)-based thermoelectric fabric for self-powered strain–temperature dual-parameter sensing via spraying and in situ bio-polymerization. Compared with ferric chloride (FeCl 3 ), used in chemical polymerization, the PEDOT thermoelectric fabric prepared by enzymatic polymerization is not doped with metal ions, making the thermoelectric performance of flexible wearable fabrics more stable. In addition, the energy-filtration effect of PEDOT and MWCNT efficiently enhanced the thermoelectric performance of the fabric. The fabric has over 320% elongation potential and excellent breathability while exhibiting excellent wearability. Moreover, the fabric-based sensor had a wide strain range (1–100%) and temperature detection limit (1 °C). In addition, fabric-based sensors were tested by sewing them directly onto clothing or attachment accessories, and showed a rapid response to changes in human joint bending and microenvironmental temperature differences. Moreover, the sensor could be integrated into an intelligent firefighting suit, to continuously and synergistically monitor health abnormalities in firefighter's body movement and temperature thresholds in the micro-environmental temperature of the suit. The developed self-powered dual-parameter wearable sensor shows fascinating potential for applications in human health monitoring, human–computer interaction devices, and intelligent robotics. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）