Nature Communications
·
2023
·Vol. 14
·期 1
·1-14
·
IF 16.6
Feng Xuezhao,Sun Daxiao,Li Yanchang,Zhang Jinpei,Liu Shiyu,Zhang Dachuan,Zheng Jingxiang,Xi Qing,Liang Haisha,Zhao Wenkang,Li Ying,Xu Mengbo,He Jiayu,Liu Tong,Hasim Ayshamgul,Ma Meisheng,Xu Ping,Mi Na
Autophagosomes are double-membrane vesicles generated intracellularly to encapsulate substrates for lysosomal degradation during autophagy. Phase separated p62 body plays pivotal roles during autophagosome formation, however, the underlying mechanisms are still not fully understood. Here we describe a spatial membrane gathering mode by which p62 body functions in autophagosome formation. Mass spectrometry-based proteomics reveals significant enrichment of vesicle trafficking components within p62 body. Combining cellular experiments and biochemical reconstitution assays, we confirm the gathering of ATG9 and ATG16L1-positive vesicles around p62 body, especially in Atg2ab DKO cells with blocked lipid transfer and vesicle fusion. Interestingly, p62 body also regulates ATG9 and ATG16L vesicle trafficking flux intracellularly. We further determine the lipid contents associated with p62 body via lipidomic profiling. Moreover, with in vitro kinase assay, we uncover the functions of p62 body as a platform to assemble ULK1 complex and invigorate PI3KC3-C1 kinase cascade for PI3P generation. Collectively, our study raises a membrane-based working model for multifaceted p62 body in controlling autophagosome biogenesis, and highlights the interplay between membraneless condensates and membrane vesicles in regulating cellular functions.
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NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
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2023
·
IF 3.6
Zhang Yuying,Zhang Pingping,Zhang Xin,Liu Ye
Honokiol, a bioactive component found in Magnolia officinalis , has shown in protecting against ischemic stroke in animal models. However, its poor water solubility has limited its clinical applications. In this study, we introduced a hydrophilic building block on the aromatic ring of honokiol, resulting in the synthesis of four new compounds (HH-A, -B, -C and -D) with significantly improved water solubility. We then investigated the neuroprotective effects of these compounds in mouse and rat models of transient middle cerebral artery occlusion/reperfusion (tMCAO/R) brain injury. Among the compounds tested, HH-A, also known as (S)-6-((3′,5-diallyl-2,4′-dihydroxy-[1,1′-biphenyl]-3-yl)amino)-6-oxohexane-1,5-diaminium chloride, showed the most promising results. HH-A was found to significantly reduced the infarct volume and brain edema in mice. It also outperformed the other three compounds and honokiol, even surpassing the effects of edaravone dexborneol. Additionally, HH-A demonstrated dose-dependent improvements in body weight, neurological deficits, and infarct volume. Further analysis in tMCAO/R rat model revealed that HH-A treatment led to significant upregulations of Nrf2 and HO-1 in the brain. HH-A also significantly reduced the expression of HNE, and exhibited anti-apoptotic effects by decreasing the expression of Bax and increasing the expression of Bcl-2. This was further supported by a decrease in the number of TUNEL positive cells. Taken together, the neuroprotective effects of HH-A may be attributed to its ability to target the Nrf2/HO-1 signaling pathway, leading to reduced oxidative stress and apoptosis in the brain. These findings suggest that HH-A has potential as a therapeutic agent for the treatment of ischemic stroke.
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CELLULOSE
·
2023
·Vol. 30
·期 18
·11845-11860
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IF 5.7
Yan Biaobiao,Gao Yilei,Fan Bingjie,Zhou Man,Liu Ying,Yu Yuanyuan,Xu Bo,Wang Qiang,Wang Ping
Although textiles decorated with electroactive materials have gained significant development in the field of wearable electronics and portable devices, the main issue is how to better exploit the synergy between conductive media for multi-scenario applications. Herein, we constructed a conductive network of 1D CNTs and 2D gallic acid-modified MXene (MG) alternating assembly of ‘wire-surface’ structure on the cotton fabric surface for wearable multifunctional personal protection. The introduction of gallic acid extended the lateral dimensions of MXene nanosheets, and the presence of CNTs protected MXene from oxidation and improved the binding of nanosheets to cotton fabric, contributing to an enhancement of the conductivity of the assembled fabric. Meanwhile, the conductivity of the assembled fabric remains stable after cyclic bending (1000 cycles) and nature storage (60 days). The assembled fabric could achieve more than 35 dB of electromagnetic interference shielding effectiveness (X-band) and outstanding infrared thermal camouflage performance. Moreover, low-radiation, Joule and solar heating are integrated to respond to the heating needs of complex scenarios and to achieve efficient utilization of energy. Based on its simple and scalable fabrication process, along with durability and comfort, the assembled cotton is expected to be promising candidates for multifunctional personal protective fabric.
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Biotechnology for Biofuels and Bioproducts
·
2023
·Vol. 16
·期 1
·1-13
·
IF 0
Zhang Xiaoling,Miao Qin,Tang Bingling,Mijakovic Ivan,Ji Xiao-Jun,Qu Lingbo,Wei Yongjun
Xylo-oligosaccharides (XOS) are considered as a promising type of prebiotics that can be used in foods, feeds, and healthcare products. Xylanases play a key role in the production of XOS from xylan. In this study, we conducted a metagenomic analysis of the fecal microbiota from dairy cows fed with different types of fodders. Despite the diversity in their diets, the main phyla observed in all fecal microbiota were Firmicutes and Bacteroidetes. At the genus level, one group of dairy cows that were fed probiotic fermented herbal mixture-containing fodders displayed decreased abundance of Methanobrevibacter and increased growth of beneficial Akkermansia bacteria. Additionally, this group exhibited a high microbial richness and diversity. Through our analysis, we obtained a comprehensive dataset comprising over 280,000 carbohydrate-active enzyme genes. Among these, we identified a total of 163 potential xylanase genes and subsequently expressed 34 of them in Escherichia coli . Out of the 34 expressed genes, two alkaline xylanases with excellent temperature stability and pH tolerance were obtained. Notably, CDW-xyl-8 exhibited xylanase activity of 96.1 ± 7.5 U/mg protein, with an optimal working temperature of 55 ℃ and optimal pH of 8.0. CDW-xyl-16 displayed an activity of 427.3 ± 9.1 U/mg protein with an optimal pH of 8.5 and an optimal temperature at 40 ℃. Bioinformatic analyses and structural modeling suggest that CDW-xyl-8 belongs to GH10 family xylanase, and CDW-xyl-16 is a GH11 family xylanase. Both enzymes have the ability to hydrolyze beechwood xylan and produce XOS. In conclusion, this metagenomic study provides valuable insights into the fecal microbiota composition of dairy cows fed different fodder types, revealing main microbial groups and demonstrating the abundance of xylanases. Furthermore, the characterization of two novel xylanases highlights their potential application in XOS production.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·
IF 5.8
Dong Lulu,Yao Zhenxing,Sun Shaohua,Wang Mingquan,Jia Ruibao
UV/peroxymonosulfate (UV/PMS) is a promising advanced oxidation technology in water treatment. This study aimed to investigate the impact of UV/PMS on humic acid (HA) and the influence of PMS dosage, pretreatment time, pH pretreatment, nitrate, nitrite, ammonium, and bicarbonate influencing factors on disinfection byproduct (DBP) formation during post-chlorination. With increased PMS dosage or pretreatment time, the UV/PMS treatment significantly reduced ultraviolet absorbance and increased mineralization. It altered the fractional constituent as humic substances were gradually transformed into building blocks and low-molecular-weight acids. However, most DBP formation increased initially and then decreased after subsequent chlorination. Rising nitrate or nitrite concentrations markedly promoted halonitromethane (HNM) formation. The presence of ammonia had a more significant impact on dichloroacetonitrile (DCAN) formation. Bicarbonate in UV/PMS pretreatment increased carbonated disinfection byproduct (C-DBP) formation, whereas it had a negligible impact on nitrogenous disinfection byproduct (N-DBP) formation. The present study revealed the impact of a series of influencing factors on DBP formation in UV/PMS reaction systems, providing comprehensive insights on applying UV/PMS in actual practice.
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CHEMICAL PAPERS
·
2023
·
IF 2.2
Hong Ye,Yu Kanping
Cotton-based clothing fabrics are welcome because they are warm, close-fitting, hygroscopic and breathable. However, surface hydrophilic hydroxyls will make them easy to stick to stains. High-cost preparing of inorganic nanoparticles can favor a building of hydrophobic coatings for them. To cheaply attain surface self-cleaning attributes of cotton-based fabrics, here, all-organic surface coatings were established utilizing surface-fluorinated uninjured polyglycidyl methacrylate (PGMA)/polyvinylidene fluoride (PVDF) co-modifying. High PVDF dosage incurred a large water contact angle (WCA) of treated fabric, and high hydrophilic attribute of pristine fabric was altered into high hydrophobic one. Antifouling attribute was acquired on the surfaces of treated fabrics, and effective ultraviolet (UV) and wearing resistances of the optimal coating/fabric composite with feeding 10 wt% of PVDF were fulfilled. The optimal fabric surface demonstrates a superhydrophobic attribute (WCA at 153.5 ± 1.5°). After UV irradiating for 24 h or surface abrasion for 100 cycles, this surface demonstrates a WCA surpassing 140° with a water sliding angle below 16°. The fiber weaving constitution and surface F assembling are critical for performance optimizing. The current research may give an effective route for large-area producing of self-cleaning cotton-based fabrics.
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Nature Communications
·
2026
·
IF 18.1
Dai Ziliang,Zhang Lili,Yu Juan,Zhu Enqing,Wang Rui,Meng Jiao,Ullah Muhammad Wajid,Khan Khalid Ali,He Wen,Fan Yimin,Wang Zhiguo
Sustainable structural fibers are highly desirable because many conventional high-performance fibers are neither renewable nor biodegradable. Bamboo original fibers, which possess a natural hollow vascular bundle architecture, represent a promising biomass-derived reinforcement but are difficult to isolate without damaging their structure. Here, we develop a mild “top-down” strategy to efficiently dissociate intact bamboo original fibers (B p OFs) from pretreated natural bamboo using a deep eutectic solvent (ChCl/OA-DES) treatment at 110 °C for 1 h. The treatment selectively weakens parenchyma cell cohesion (elastic modulus reduced from 6.24 to 3.14 GPa) while preserving fiber cell stiffness (15.04 to 15.92 GPa), enabling rapid separation of vascular bundles through subsequent CH₃COOH/H₂O₂ delignification and simple roller compaction. Controlled hydrogen-bond reconstruction during drying enhances mechanical performance, yielding fibers with an apparent tensile strength of 1947 ± 217 MPa while retaining the native hollow structure. Analysis of the load-bearing cell wall further reveals an intrinsic cell-wall stress of 2636 ± 197 MPa, indicating highly efficient stress transfer within the cellulose framework. The preserved tubular architecture provides low density, flexibility, and favorable interfacial interactions with polymer matrices. When incorporated into epoxy resin, B p OFs enhance bending strength and impact resistance, demonstrating their potential as lightweight, renewable reinforcement for high-performance composites.
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Chemical and Biological Technologies in Agriculture
·
2023
·Vol. 10
·期 1
·1-16
·
IF 6.6
Zhong Ximing,Lan Ruopeng,Su Guofeng,Hao Li,Xu Hua,Zhou Hongjun,Zhou Xinhua
Background The growing escalation of soil salinization is tremendously threatening the global food security and the development of sustainable agriculture. To address the worldwide predicament caused by salt stress toward crops, combining nanotechnology with the merits of plant hormone may become an efficient and effective approach. Results In this work, a sustained release agent system (BR@MSN) was developed by loading brassinolide (BR) to mesoporous silica nanoparticles (MSN) to enhance the salt stress resistance of cucumber seeds and seedlings. The obtained BR@MSN agent was about 120 nm. As an endogenous plant hormone, promotion in crop growth was found at low BR concentration. Due to the sustained release property, BR@MSN avoided excessive BR exposure to seeds and seedlings to cause inhibitory effects. After the soil application of BR@MSN, the promotion effect from BR combined with the regulation enhancement from MSN nanocarrier improved the seed germination rate by 11.76% under saline environment. Compared with the same BR concentration (2.0 mg/L), BR@MSN increased the seed germination rate even by 1324.29%. In addition, remarkable wettability on foliar surfaces was found, and the foliar application of BR@MSN significantly enhanced the salt stress resistance of cucumber seedlings by alleviating the accumulation of reactive oxygen species (ROS) and increasing the cell viability along with the improvement in superoxide dismutase (SOD) activity (234.11%), the decrease in malondialdehyde (MDA) content (61.30%), and the increase in chlorophyll content (110.88%). Conclusions The newly developed BR@MSN agents could effectively enhance the salt stress resistance of crop seeds and seedlings, and their applications significantly improved the seed germination rate and seedling growth. The remarkable efficacy makes this BR@MSN agent system potential in agricultural field for enhancing the salt stress resistance of crops and facilitating the development of sustainable agriculture. Graphical Abstract
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JOURNAL OF POLYMER RESEARCH
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2023
·Vol. 30
·期 12
·1-12
·
IF 2.8
Huang Baoli,Ma Meng,Wang Yueyin,Chen Si,Shi Yanqin,He Huiwen,Wang Xu
Rational design morphology of flame retardant is crucial to achieving a balance between satisfactory flame retardancy and superior mechanical properties. However, there are few studies focused on the morphology of flame retardants, and how to achieve the aim of satisfactory flame retardancy yet low mechanical properties loss is still a challenge. Herein, aluminum diethylphosphinate ( ADP) with different morphologies were obtained through hydrothermal synthesis method via adjusting the releasing rate of Al 3+ , molar ratio (OH − : Al 3+ ), and changing the type of acid. The morphology of ADP was successfully regulated from random (ADP-1), rod-like (ADP-2) to flower-like (ADP-3). The formation mechanism of different morphology was discussed in detail. The TG results indicated that all ADP have high thermal stability. Therefore, ADP as flame retardant was used for modification of TPU. Compared to neat TPU, TPU/ADP composites present lower initial decomposition temperature and higher char residual. With addition of 30% ADP-2 the LOI value increased from 22.5 to 28.0%, achieved the UL-94 V-2 rating, respectively. THR was 25.6% lower than that of virgin TPU, exhibiting higher fire safety performance. Meanwhile the tensile strength of TPU composite with ADP-2 (10.8 ± 0.4) is higher than that of the composites with ADP-1 (8.7 ± 0.9), and ADP-3 (7.9 ± 0.5) at content up to 30%. This work provides a promising strategy to tune the morphology of flame retardant and compromise the contradiction of poor mechanical properties with high fire safety of polymer composite.
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Nature Protocols
·
2023
·
IF 14.8
Li Hao,Zhu Lele,Fan Ruixiao,Li Zheng,Liu Yifan,Shaheen Aaqib,Nie Fang,Li Can,Liu Xuqin,Li Yuanyuan,Liu Wenjuan,Yang Yingying,Guo Tutu,Zhu Yu,Bu Mengchen,Li Chenglin,Liang Huihui,Bai Shenglong,Ma Feifei,Guo Guanghui,Zhang Zhen,Huang Jinling,Zhou Yun,Song Chun-Peng
Breeding new and sustainable crop cultivars of high yields and desirable traits has been a major challenge for ensuring food security for the growing global human population. For polyploid crops such as wheat, introducing genetic variation from wild relatives of its subgenomes is a key strategy to improve the quality of their breeding pools. Over the past decades, considerable progress has been made in speed breeding, genome sequencing, high-throughput phenotyping and genomics-assisted breeding, which now allows us to realize whole-genome introgression from wild relatives to modern crops. Here, we present a standardized protocol to rapidly introgress the entire genome of Aegilops tauschii , the progenitor of the D subgenome of bread wheat, into elite wheat backgrounds. This protocol integrates multiple modern high-throughput technologies and includes three major phases: development of synthetic octaploid wheat, generation of hexaploid A. tauschii –wheat introgression lines (A-WIs) and homozygosis of the generated A-WIs. Our approach readily generates stable introgression lines in 2 y, thus greatly accelerating the generation of A-WIs and the introduction of desirable genes from A. tauschii to wheat cultivars. These A-WIs are valuable for wheat-breeding programs and functional gene discovery. The current protocol can be easily modified and used for introgressing the genomes of wild relatives to other polyploid crops.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-10
·
IF 16.6
Ai Liqing,Lin Weikang,Cao Chunyan,Li Pengyu,Wang Xuejiao,Lv Dong,Li Xin,Yang Zhengbao,Yao Xi
The rapid-developing soft robots and wearable devices require flexible conductive materials to maintain electric functions over a large range of deformations. Considerable efforts are made to develop stretchable conductive materials; little attention is paid to the frequent failures of integrated circuits caused by the interface mismatch of soft substrates and rigid silicon-based microelectronics. Here, we present a stretchable solder with good weldability that can strongly bond with electronic components, benefiting from the hierarchical assemblies of liquid metal particles, small-molecule modulators, and non-covalently crosslinked polymer matrix. Our self-solder shows high conductivity (>2×10 5 S m −1 ), extreme stretchability (~1000%, and >600% with chip-integrated), and high toughness (~20 MJ m −3 ). Additionally, the dynamic interactions within our solder’s surface and interior enable a range of unique features, including ease of integration, component substitution, and circuit recyclability. With all these features, we demonstrated an application as thermoforming technology for three-dimensional (3D) conformable electronics, showing potential in reducing the complexity of microchip interfacing, as well as scalable fabrication of chip-integrated stretchable circuits and 3D electronics.
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NATURE MATERIALS
·
2023
·
IF 41.2
Huang Xiaoyan,Zhang Shuai,Zhang Pei,Zhu Yujie,Xie Jiaye,Yang Mingcong,Han Lu,Hu Jun,Li Qi,He Jinliang
Dielectric polymers are ubiquitous as electrical insulation in electronic devices and electrical systems. Electrical degradation of dielectric polymers tends to initiate catastrophic failure of numerous devices and systems, but its detection and early warning remain challenging. Here we report a general material strategy that signals the electrical degradation of dielectric polymers by autonomously presenting a visually discernible warning in the form of a pronounced colour change. This colour change is induced by the chromogenic response of molecular indicators blended with the polymer, which are chemically activated by the oxygen radicals generated in situ during the electrical degradation of the polymer. We unveil that the structural degradation and electrical properties of the dielectric polymer are quantitatively correlated with the colour difference. Such a chromogenic process is autonomous without the need of human intervention or other external energy, thus offering the convenience to lower or even eliminate the risk of dielectric failure.
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危险品化学品经营许可证（带存储）