ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Quanyu Wang,Zichao Wang,Zhiqian Li,Pu Liu,Zonglin Pan,Zhaoqian Xie,Yongxin Song,Xuegang Zhang,Ruisong Xu
Obtaining multi-mode tactile information with one sensor in deep sea is greatly needed yet still challenging. In this paper, a flexible deep sea tactile sensor is reported, capable of detecting object's conductivity, hardness, pressing pressure and position simultaneously just by one touch. The sensor works by using single-walled carbon nanotubes doped thermoplastic polyurethane elastomer (SWNTs-TPU film) for differentiating object's conductivity based on the work function difference induced electron transfer. A porous anionic aliphatic waterborne polyurethane film containing carboxyl functional groups (C-WPU) is used for generating ionic currents under pressing to determine the pressing pressure and position and recognize the relative hardness of an object. It is found that the magnitude of current signal generated by a metallic object is much larger than that of an insulative object. The signal magnitude increases with the increase in the applied pressure, the pressing area and the film thickness. Furthermore, completely encapsulating the C-WPU film and introducing carboxylic functional groups significantly improve the sensitivity (maximum 1.2 kPa −1 ). The response time and recovery time of sensing signals are 150 and 530 ms respectively. A pressure detection range of 45–625 kPa is achieved, with a pressure detection resolution of 9.2 kPa. The signal magnitude is decreased only by 10.54% when the sensor is placed under a simulated 100 m water depth. Proof of concept demonstration of underwater object discrimination and hardness recognition with this sensor placed on a mechanical finger of a ROV (Remotely Operated Vehicle) is successfully achieved. This work provides a new solution for multi-modal tactile information sensing in deep-sea environment.
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产品关联
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-13
·
IF 4.259
Wang Hetong,Song Laiyu,Ju Wenhui,Wang Xuguang,Dong Lu,Zhang Yining,Ya Ping,Yang Chun,Li Fasheng
PM 2.5 is the main particulate air pollutant whose aerodynamic diameter is less than 2.5 micron. The inflammation of various respiratory diseases are associated with PM 2.5 inhalation. Pro-inflammatory cytokine IL-1β generated from effected cells usually plays a crucial role in many kinds of lung inflammatory reactions. The exacerbation of Th immune responses are identified in some PM 2.5 related diseases. To elucidate the underlying mechanism of PM 2.5 -induced acute lung inflammation, we exposed Balb/c mice to PM 2.5 intratracheally and established a mice model. Acute lung inflammation and increased IL-1β expression was observed after PM 2.5 instillation. Regulatory factors of IL-1β (TLR4/MyD88 signaling pathway and NLRP3 inflammasome) participated in this lung inflammatory response as well. Treatment with compound essential oils (CEOs) substantially attenuated PM 2.5 -induced acute lung inflammation. The decreased IL-1β and Th immune responses after CEOs treatment were significant. PM 2.5 may increase the secretion of IL-1β through TLR4/MyD88 and NLRP3 pathway resulting in murine airway inflammation. CEOs could attenuate the lung inflammation by reducing IL-1β and Th immune responses in this model. This study describes a potentially important mechanism of PM 2.5 -induced acute lung inflammation and that may bring about novel therapies for the inflammatory diseases associated with PM 2.5 inhalation.
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DOI
PubMed
高影响因子
STAR Protocols
·
2023
·
IF 0
Shu-qin Cao,He-Ling Wang,Konstantinos Palikaras,Nektarios Tavernarakis,Evandro Fei Fang
Summary Here, we present an olfactory-dependent chemotaxis assay for evaluating changes in memory-like behavior in both wild-type and Alzheimer’s-disease-like C. elegans models. We describe steps for synchronizing and preparing C. elegans populations and for performing isoamyl alcohol conditioning during starvation and chemotaxis assaying. We then detail counting and quantification procedures. This protocol is applicable to mechanistic exploration and drug screening in neurodegenerative diseases and brain aging.
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DOI
PubMed
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2022
·
IF 3.322
Wei Liu,Zhen Lu,Shan Yuan,Xinglin Jiang,Mo Xian
N–N bonded compounds are a relatively small but valuable group of natural products with a variety of important biological activities, including anti-inflammatory, anti-tumor, anti-bacterial and anti-oxidant activities. Currently, the synthesis of natural products containing N–N bonds is mainly based on chemical synthesis, but the chemical synthesis of N–N bonds may have safety issues and raw material sustainability problems. A variety of N–N bond biosynthetic mechanisms exist in nature, including comproportionation, rearrangement, and radical recombination reactions, which provide ideas for the biosynthesis of many N–N bond compounds. But at this stage, only a few N–N bond synthases have been reported, and difficulties exist in the biosynthesis of N–N bond-containing compounds. The new N–N-bond synthase PAI2, which was found to catalyze the synthesis of piperazinic acid, expands the family of N–N-bond synthases and provides better catalytic activity and different enzymatic properties for the substrate L-N 5 -hydroxyornithine than the known enzyme KtzT derived from the piperazinic acid biosynthetic gene cluster of Kutzneria sp. 744. Moreover, PAI2 was found to have the ability to catalyze the formation of C–N bonds in Pro .
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DOI
PubMed
高影响因子
Aging-US
·
2021
·
IF 5.682
Longlong Gong,Zhengzhi Zou,Lei Liu,Shuang Guo,Da Xing
Ameliorating hyperglycemia and insulin resistance are major therapeutic strategies for type 2 diabetes. Previous studies have indicated that photobiomodulation therapy (PBMT) attenuates metabolic abnormalities in insulin-resistant adipose cells and tissues. However, it remains unclear whether PBMT ameliorates glucose metabolism in skeletal muscle in type 2 diabetes models. Here we showed that PBMT reduced blood glucose and insulin resistance, and reversed metabolic abnormalities in skeletal muscle in two diabetic mouse models. PBMT accelerated adenosine triphosphate (ATP) and reactive oxygen species (ROS) generation by elevating cytochrome c oxidase (C c O) activity. ROS-induced activation of phosphatase and tensin homolog (PTEN)/ protein kinase B (AKT) signaling after PBMT promoted glucose transporter GLUT4 translocation and glycogen synthase (GS) activation, accelerating glucose uptake and glycogen synthesis in skeletal muscle. C c O subunit III deficiency, ROS elimination, and AKT inhibition suppressed the PBMT effects of glucose metabolism in skeletal muscle. This study indicated amelioration of glucose metabolism after PBMT in diabetic mouse models and revealed the metabolic regulatory effects and mechanisms of PBMT on skeletal muscle.
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Bing Wang,Lei Zhang,Guo Yin,Jue Wang,Ping Wang,Tiejie Wang,Jiangwei Tian,Xie-an Yu,Huachao Chen
Cystatin C (Cys C) has been considered as a novel biomarker of kidney disease, which is thought to be a better indicator of glomerular filtration rate than creatinine (Scr) in the prediction of acute kidney injury (AKI). Hence, there is strong need to develop a precise, rapid and simple detection method for Cys C. Here we reported a Arg-liposome-amplified colorimetric immunoassay for the detection of Cys C to predict AKI. Cys C antibodies are conjugated on the surface of magnetic beads (MBs) and arginine (Arg)-loaded liposomes to form Ab1-MBs and Ab2-Arg-liposomes, respectively. When Ab1-MBs captured Cys C, Ab2-Arg-liposomes are added and incubated to form the immuno-sandwich complex. After magnetic separation, the surfactant Triton ×100 is added to damage the liposomes, leading to the release of Arg which can induce the gold nanoparticles aggregation. Therefore, the discoloration can be used for visual and quantitative detection of Cys C. Notably, the method has a linear relation in the range of 10–100 μg/L for Cys C with a limit of detection 4.32 μg/L, which is lower than some of the previous reports. In addition, the AKI mice serum samples were tested by the developed method, which were in good agreement with ELISA results. More intriguingly, the results of cisplatin induced acute kidney injury in mice showed that the method could be used to evaluate the protective effect of astragalus membranaceus (AM) on AKI by detecting Cys C in serum, providing a new strategy for screening renal protective drugs. Accordingly, a rapid and highly sensitive Cys C detection system was established with great potential for clinical diagnostics.
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DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2022
·
IF 4.446
Lankai Su,Kun Chen,Shu Bai,Linling Yu,Yan Sun
Environmental-friendly treatment of poly (ethylene terephthalate) (PET) is important for the circular plastic economy, and enzymatic degradation provided a promising approach. However, enzyme binding toward the PET surface is a rate-limiting factor in PET hydrolysis . Thus, a 16-residue peptide (αSP) from the C-terminal sequence of α-synuclein was used to fuse to the C-terminal of a variant (ICCG) of the leaf-branch compost cutinase , and supposed to serve as an anchor peptide for PET degradation. The fusion enzyme (ICCG-αSP) was characterized to keep the same secondary and tertiary structures as ICCG, but enhanced enzyme binding onto bis(2-hydroxyethyl) terephthalate and PET, respectively. Moreover, αSP-fusion enhanced enzyme expression by over 50%, and increased the catalytic efficiency of p -nitrophenyl butyrate by 20% without compromising the enzyme stability . For PET degradation, ICCG-αSP was 3.89 times more active than ICCG by 3-h reaction at 50 °C, and exhibited 1.05–1.45 times higher degradation activity than ICCG in 4-d reaction at 50–70 °C, and pH 7.0 to pH 9.0, respectively. Furthermore, αSP-fusion lowered the optimal long-term degradation temperature from 65 °C to 50 °C at pH 8.0. Therefore, αSP-fusion was effective to increase the hydrolytic efficiency of PET hydrolases by increasing the degradation efficiency and decreasing the degradation temperature.
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DOI
高影响因子
MICROBIOLOGICAL RESEARCH
·
2023
·
IF 6.7
Xiao-Yu Sun,Jie Deng,Chenhui Zhang,Sin-Yee Fung,Kam-Leung Siu,Ying-Ying Cheng,Liumei Ye,Jiaoxia Qin,Ke Wang,Jiu-Xin Qu,Wenying Gao,Fuxiang Wang,Dong-Yan Jin,Liang Yang
C-di-GMP is a bacterial second messenger implicated in the regulation of many key functions including antibiotic tolerance and biofilm formation. Our understanding of how c-di-GMP exerts its action via receptors to modulate different biological functions is still limited. Here we used a c-di-GMP affinity pull-down assay coupled to LC-MS/MS to identify c-di-GMP-binding proteins in the opportunistic pathogen Stenotrophomonas maltophilia. This analysis identified Smlt3238 (SodA), a protein of the superoxide dismutase family, as a c-di-GMP-binding protein. Microscale thermophoresis showed that purified SodA protein bound c-di-GMP with an estimated dissociation constant (Kd) value of 141.5 μM. Using various in vivo and in vitro experiments, we demonstrated that c-di-GMP modulates the enzyme activity of SodA directly. Circular dichroism experiments revealed that SodA protein gradually altered its basic structure with increasing levels of c-di-GMP. Phenotypic experiments conducted in the presence of a range of intracellular c-di-GMP levels showed that SodA function is modulated by c-di-GMP. The findings thus identify a novel c-di-GMP binding protein that governs oxidative stress tolerance in S. maltophilia .
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Xiaolei Wu,Hanqing Zhao,Junmin Xu,Zhuangfei Zhang,Weixia Sheng,Shuge Dai,Tingting Xu,Sen Zhang,Xinchang Wang,Ye Wang,Xinjian Li
Searching for low-cost, high capacity, stable and well-designed cathode materials with good rate capability and long-life cycling performances is our long-term goal for rechargeable lithium/sodium batteries . In this work, a simple and scalable strategy is developed to synthesize metal-organic frameworks (MOFs) derived Fe 7 S 8 /C composites by sulfurizing Fe-MIL-88A MOFs. The synthesized Fe 7 S 8 /C composites are of rod-like morphology with the well-dispersed Fe 7 S 8 nanoparticles in porous carbon matrix. Such a structure could offer distinct advantages for Fe 7 S 8 -based electrode materials for both lithium and sodium batteries . When evaluated as a cathode material for rechargeable Fe 7 S 8 /Li batteries , the Fe 7 S 8 /C composites exhibit a long and flat discharge plateau around 1.5 V and delivers a high specific discharge capacity of 473 mA h g −1 at 0.1 A g −1 after 100 cycles with excellent rate capability and long-life cycling performance at high current density (~301 mA h g −1 after 1000 cycles at 1.0 A g −1 ). Moreover, the Fe 7 S 8 /C composites could also exhibit high specific capacities, good rate performance and high cycling stability as an anode material for sodium ion batteries. Our findings demonstrate that Fe 7 S 8 /C composites with rod-like morphology are promising electrode materials for lithium/sodium batteries.
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DOI
高影响因子
HELVETICA CHIMICA ACTA
·
2012
·Vol. 95
·期 2
·320-326
·
IF 1.478
Lianbao Ye,Yuanxin Tian,Zhonghuang Li,Jiajie Zhang,Shuguang Wu
Since deregulation of the tyrosine-kinase receptor c-Met is implicated in several human cancers and is an attractive target for small-molecule-drug discovery, we report herein the synthesis of 2,3,4,5-tetrahydro-8-[1-(quinolin-6-ylmethyl)-1 H -1,2,3-triazolo[4,5- b ]pyrazin-6-yl]-1 H -pyrido[4,3- b ]indoles 4a – 4c and 2,3,4,5-tetrahydro-8-[3-(quinolin-6-ylmethyl)-1,2,4-triazolo[4,3- b ]pyridazin-6-yl]-1 H -pyrido[4,3-b]indoles 5a – 5c . These indole derivatives demonstrated inhibition of c-Met kinase activity. Concurrently, five key intermediates were synthesized. These compounds could be prepared in good yields.
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Yanyan Kong,Jiaxiang Sun,Ligang Gai,Xiaojuan Ma,Jianhua Zhou
Aqueous rechargeable batteries (ARBs) based on migration of two types of shuttle ions not only enrich the battery families but also make it more available for ARBs with operation potential higher than 1.2 V. In this paper, we report on electrochemical properties of ARBs in configuration of NaTi 2 (PO 4 ) 3 /C||LiMn 2 O 4 , i.e. NTP/C||LMO. NTP/C was prepared through a simple and scalable co-precipitation post annealing treatment method and featured with hierarchical structure, high specific surface area, and abundant mesopores. The electrochemical properties of individual anode and cathode were examined in 1 mol L −1 Li 2 SO 4 , 1 mol L −1 Na 2 SO 4 , and 0.5 mol L −1 Li 2 SO 4 /0.5 mol L −1 Na 2 SO 4 mixed electrolyte, respectively. It is found that NTP/C exhibits superior operation in 1 mol L −1 Na 2 SO 4 and inferior operation in 1 mol L −1 Li 2 SO 4 while the situation is contrary for LMO. However, NTP/C||LMO full cells with Li + /Na + -mixed electrolyte exhibit enhanced electrochemical performance, presenting an anode-dependant electrochemical behavior. When operating at 1C, NTP/C||LMO with Li + /Na + -mixed electrolyte can deliver a discharge specific capacity ( C dis ) of 104.6 mA h g −1 based on the weight of NTP/C, and a specific energy ( E cell ) of 59.5 W h kg −1 with corresponding specific power of 76.8 W kg −1 based on the total weight of active electrode materials. After 220 cycles at 1C, the C dis and E cell remain 75.7 mA h g −1 and 43.0 W h kg −1 , respectively.
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高影响因子
SOLID STATE IONICS
·
2017
·
IF 2.354
Zhi Zhou,Yiran Li,Mao Xia,Nan Zhou
In this paper, Li 1–x Ti 2 (PO 4 ) 3 /C composites (x = 0, 0.03, 0.05, 0.07) were synthesized by sol-gel approach, and the influence of Li vacancy on electrochemical performance of LiTi 2 (PO 4 ) 3 /C anode for aqueous rechargeable lithium battery was studied. Li vacancy hardly influences the crystal structure and morphology of the composite. Electrochemical measurement results demonstrated that Li 1–x Ti 2 (PO 4 ) 3 /C exhibited better performance compared with pristine LiTi 2 (PO 4 ) 3 /C, and Li 0.95 Ti 2 (PO 4 ) 3 /C has the best properties. Li 0.95 Ti 2 (PO 4 ) 3 /C delivered the discharge capacity of 104.3, 92.6, and 71.8 mAh g − 1 at 0.2, 5, and 20 C (1 C = 150 mA g − 1 ), 4.1, 27.3, and 34.4 mAh g − 1 larger than that of Li 0.95 Ti 2 (PO 4 ) 3 /C at corresponding rates, respectively. After 1000 cycles at 5 C, the discharge capacity retention for Li 0.95 Ti 2 (PO 4 ) 3 /C can reach 75.3%, 15.4% larger than that for LiTi 2 (PO 4 ) 3 /C. Our study reveals that Li vacancy is an efficient method to enhance the electrochemical properties of LiTi 2 (PO 4 ) 3 -based materials.
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高影响因子
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