INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2022
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IF 4.907
Xiaoyong Song,Zihan Zhang,Yanran Dai,Deshou Cun,Baihui Cui,Yuewei Wang,Yaocheng Fan,Haibin Tang,Liang Qiu,Feihua Wang,Dongru Qiu,Wei Liang
Di-(2-ethylhexyl) phthalate (DEHP), the most extensively used phthalate acid esters (PAEs), poses a potential risk to human and environment. A novel bacterial strain , Rhodococcus sp. LW-XY12, with efficient PAE-degrading capability, was isolated from activated sludge. Strain LW-XY12 could degrade 96.91 ± 0.68% of DEHP (500 mg L −1 ) within 32 h. The degradation curves of DEHP (50–1500 mg L −1 ) and PAE mixture (500 mg L −1 each) fitted well with the modified Gomperz kinetics model. DEHP metabolic pathway was reconstructed by using genome annotation and metabolic intermediate analyses. The DEHP metabolic pathway might comprise de-esterification and β-oxidation. Homologous modeling and molecular docking analysis revealed that DEHP and MEHP (mono-(2-ethylhexyl) phthalate) could be bound to putative carboxylesterase (KXC42_04905) via hydrogen bonding and hydrophobic interaction. A conserved catalytic triad (Ser195-Glu319-His412) might act as an active protein pocket and catalyze the hydrolysis of DEHP. Carboxylesterase (KXC42_04905) could hydrolyze ester bond and a possible mechanism underlying ester bond hydrolysis catalyzed by the carboxylesterase was proposed. Protocatechuate 3,4-dioxygenase, 3-oxoadipate CoA-transferase, benzoate 1,2-dioxygenase, catechol 1,2-dioxygenase, and catechol 2,3-dioxygenase transcripts were detected and significantly up-regulated under DEHP induction. This study sheds light on the functional genes associated with DEHP degradation and metabolic mechanism in this versatile bacterium for degradation of PAEs.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2022
·Vol. 29
·期 13
·19913-19917
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IF 5.19
Zhang Jun,Zhong Shu-shu,Zhao Ke-meng,Liu Ze-hua,Dang Zhi,Liu Yu
Steroid arylsulfatase is an important enzyme in human, which plays an important role in dynamic equilibrium of natural estrogens. On the other hand, sulfite can be endogenously produced as a consequence of human body’s metabolism of sulfur-containing amino acids, while its main sources to human are mainly derived from food as it is a widely used additive. Sulfite-sensitivity is a well-known phenomenon to a small proportion of populations. However, its potential adverse effects on healthy individuals have been hardly reported. It was for the first time reported in this study that sulfite could effectively inhibit arylsulfatase, and its IC50 values for the snail- and human urine–derived arylsulfatase were determined to be 71.9 and 142.8 µM, which were lower than the concentration of sulfite in some healthy population. Consequently, it appears that sulfite might disrupt estrogen homeostasis in human, and this deserves further investigation.
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ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Xue Wang,Futing Wen,Liu He,Jiangyu Su,Ping Jiang,Daiping He
Defects engineering as an effective strategy was widely utilized to modulate catalytic performance for heterogeneous catalysts through tuning surface electronic surface and creating more active sites. In this work, porous Co–Mn oxide nanosheets with abundant oxygen vacancies (OVs-CMO NSs) were prepared via a simple “one-stone-two-birds” method. OVs were verified by various characterizations and found more favorable for O 2 absorption and activation to produce more reactive O2•- radical, resulting in an improved oxidase-like activity in initiating 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation , with a lower K m value of 0.00717 mM and a higher V max of 2.086 × 10 −7 M/s. Since heparin (Hep) shows an inhibition effect on OVs-CMO NSs-TMB chromogenic system due to strong absorption, a colorimetric sensing platform for Hep detection was established. The present colorimetric detection shows high sensitivity, good selectivity and reliability for normal goat serum. This work not only provides a high-performance non-precious metal oxidase mimic prepared by simple surface defect engineering, but also builds a sensitive colorimetric method for Hep detection.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 2
·1-12
·
IF 6.408
Li Guofu,Xie Guoqiang,Gong Chong,Chen Di,Chen Xing,Zhang Qian,Dong Hongzhou,Zhang Yingchao,Li Chengjie,Hu Jing,Chen Yingjie,Yu Liyan,Dong Lifeng
Chains of nickel nanoparticles coated with few nitrogen-doped graphitic carbon layers (Ni@NC) are synthesized by hydrogen-assisted pyrolysis of Ni-ZIF. Hydrogen and temperature can play key roles in the formation of oriented Ni@NC nanoparticle chains, and carbon shells can protect Ni nanoparticles from external oxidation and aggregations. Under the optimized potential (0.60 V vs. Ag/AgCl), the Ni@NC7H nanoparticle chains obtained at 700 °C under H 2 /Ar atmosphere (Ni@NC7H) demonstrate outstanding performances, such as high sensitivity of 1.44 mA mM −1 cm −2 (RSD = 1.0%), low detection limit of 0.34 μM (S/N = 3), broad linear range from 1 μM to 1.81 mM, and excellent application potential in artificial sweat and human serum. Therefore, the findings above indicate that this study will provide a general methodology for the synthesis of chains-like core–shell nanoparticle electrocatalysts for non-enzymatic glucose detection. Graphical abstract
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CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Yang Xu,Na Liang,Jiyang Liu,Xianfeng Gong,Pengfei Yan,Shaoping Sun
In this study, cetyl 4-formylbenzoate alkyl and 4-(2-hydroxyethoxy) benzophenonesalicylaldazide modified biotinylated chitosan (CS-BT-HBS-CB) featured with aggregation-induced emission (AIE) characteristic, active tumor-targeting ability and pH-responsive drug release property was designed and synthesized. The polymer was fabricated by introducing hydrophobic segment, tumor targeting ligand, acid-sensitive bond and AIE fluorophore to the backbone of chitosan. Due to its amphiphilicity, the polymer could self-assemble into micelles and encapsulate paclitaxel (PTX) to form PTX-loaded CS-BT-HBS-CB micelles. The mean size of the micelles was 167 nm, which was beneficial to the EPR effect . Moreover, with the help of above functional groups, the micelles exhibited excellent AIE effect, triggered drug release behavior by acidic condition, selective internalization by MCF-7 cells and excellent cellular imaging capability. In vivo studies revealed that the PTX-loaded CS-BT-HBS-CB micelles could enhance the antitumor efficacy with low systemic toxicity. This micellar system would be a potential candidate for cancer therapy and bioimaging.
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Food & Nutrition Research
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2022
·
IF 3.221
Tian Su,Zhen Wang,Zhengyi Zhang,Zhanwu Hou,Xiao Han,Fei Yang,Huadong Liu
Background Resveratrol, a well-known natural compound and nutrient, activates the deacetylation ability of SIRT1, demonstrating p53-dependent apoptosis functions in many diseases. However, the nascent proteomic fluctuation caused by resveratrol is still unclear. Objective In this study, we investigated the effect of resveratrol on the nascent proteome and transcriptome initiated by SIRT1 activation, and we explored the mechanism of resveratrol in HEK 293T cells. Methods Bioorthogonal noncanonical amino acid tagging (BONCAT) is a method used to metabolically label nascent proteins. In this strategy, L-azidohomoalanine (AHA) was used to replace methionine (Met) under different conditions. Taking advantage of the click reaction between AHA and terminal alkyne- and disulfide-functionalized agarose resin (TAD resin), we were able to efficiently separate stimulation responsive proteins from the pre-existing proteome. Resveratrol responsive proteins were identified by Liquid Chromatograph-Mass Spectrometer/Mass Spectrometer (LC-MS/MS). Furthermore, changes in mRNA levels were analyzed by transcriptome sequencing. Results Integrational analysis revealed a resveratrol response in HEK 293T cells and showed that Hsp60 was downregulated at both the nascent protein and mRNA levels. Knockdown of SIRT1 and Hsp60 provides evidence that resveratrol downregulated Hsp60 through SIRT1 and that Hsp60 decreased p53 through the Akt pathway. Conclusions This study revealed dynamic changes in the nascent proteome and transcriptome in response to resveratrol in HEK 293T cells and demonstrated that resveratrol downregulates Hsp60 by activating SIRT1, which may be a possible mechanism by which resveratrol prevents p53-dependent apoptosis by regulating Hsp60.
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高影响因子
DALTON TRANSACTIONS
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2022
·Vol. 51
·期 20
·7950-7956
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IF 4.569
Liuyong Chen,Jun Yang,Wenqian Yang,Jiahui Xian,Guangqin Li
Photochemical CO2 conversion into carbon fuel is a promising route to explore renewable energy and relieve climate change. However, it is still a key challenge to achieve high selectivity to CO and simultaneously achieve high conversion efficiency in photochemical CO2 reduction. Herein, we demonstrate the effect of Ni metal centers as catalytic active sites for the photocatalytic conversion of CO2 to CO by designing and constructing Ni metal–organic framework (Ni-MOF) materials. In pure CO2, Ni-MOF catalyst exhibits outstanding performance for visible-light-driven reductive CO2 deoxygenation with a high CO evolution rate of 19.13 μmol h−1 (per 1 mg of catalyst) and CO selectivity of 91.4%, which exceeds those of most reported systems. Upon using isostructural Co-MOF as the catalyst to replace Ni-MOF, a moderate performance towards CO2 photoreduction and low CO selectivity (40.1%) were observed, implying that the performance of CO2 photoreduction and CO selectivity are dependent on unsaturated metal centers.
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RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2022
·Vol. 36
·期 13
·e9314
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IF 2.586
Dongping Rao,Baichun Wang,Huizhen Zhong,Yinghua Yan,Chuan-Fan Ding
Rationale Cervical cancer is one of the most common malignant tumors in women, and it is essential to explore potential biomarkers such as glycopeptides closely related to cancer in physiological samples of cervical cancer patients. Sample pretreatment is required before direct detection using mass spectrometry because there are certain limitations. Meanwhile, it is still highly desired to promote the functionalization and application of metal–organic framework (MOF)-derived materials. Methods Using a post-synthesis modification method, a novel type of boric acid-functionalized MOF probe (designated as UiO-66@PEI@Au@B(OH) 2 ) is prepared for recognition of glycopeptides. The results are obtained using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nano-liquid chromarography–tandem mass spectrometry. Results The UiO-66@PEI@Au@B(OH) 2 probe exhibits a low detection limit (0.6 fmol μL −1 ), an excellent recovery rate, comparatively good reusability and selectivity (HRP digests:BSA digests = 1:500). When UiO-66@PEI@Au@B(OH) 2 is used to selectively capture glycopeptides from the serum of a healthy person and a cervical cancer patient, 101 glycopeptides corresponding to 54 glycoproteins and 108 glycopeptides corresponding to 57 glycoproteins are detected, respectively. Conclusions The successful preparation of UiO-66@PEI@Au@B(OH) 2 provides a path for the investigation of the functionalization of MOF-derived materials. The excellent performance of UiO-66@PEI@Au@B(OH) 2 not only demonstrates the huge potential of functionalized MOFs in the glycoproteome, but also opens up new phases of the application of MOF-based materials.
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ADVANCED POWDER TECHNOLOGY
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2022
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IF 4.969
Yan Wang,Kangkang Miao,Weixing Zhao,Hongbo Jiang,Liangliang Liu,Dengwei Hu,Bin Cui,Yaping Li,Yue Sun
In this study, nanoparticle-assembled Bi 25 FeO 40 materials were synthesized by a novel and simple process, and their microstructure was regulated by different methods of adding Bi 3+ and Fe 3+ . The novel nanoparticle-assembled tetrakaidekahedron Bi 25 FeO 40 materials exhibited more oxygen vacancy and larger specific surface area than irregular or cubic-like Bi 25 FeO 40 microcrystals and revealed better photo-Fenton catalytic oxidation activity toward degrading Rhodamine B (RhB) pollutants. These novel materials activated H 2 O 2 in visible light to produce non-free radicals 1 O 2 and hole (h + ) active oxidative species that dominated the catalytic degradation of RhB. Complete degradation of RhB was achieved after 1 h. The possible reaction mechanisms of the photo-Fenton catalytic system are discussed in detail by radical scavenging tests, X-ray phosphorescence analysis, and electron paramagnetic resonance spectroscopy . The results provided a way to rationally explore and construct photo-Fenton catalysts for wastewater treatment .
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CHEMOSPHERE
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2022
·
IF 8.943
Ting Chen,Zhiliang Zhu,Yue Wang,Hua Zhang,Yanling Qiu,Daqiang Yin
Facilitating reactive oxygen species (ROS) generation is an effective way to promote the heterogeneous catalytic efficiency for organics removal. However, the metal leaching in metal-based catalysts and the low activity of non-metallic materials restrict ROS production. In this work, the purpose was achieved by loading a small amount of spinel CuFe 2 O 4 onto porous carbon nitride substrate. The synthesized CuFe 2 O 4 @O–CN composite first to activate peroxymonosulfate (PMS), which produce a plenty of ROS (•OH, SO 4 • − and 1 O 2 ) for organics removal, leading to highly oxidation for diverse organics. Through the comparative analysis of the surface composition before and after reaction, we found that the interface multi-electron transfer routs, including surface Cu(II)/Cu(I), Fe(III)/Fe(II) and their cross interaction, participated in the redox cycle, giving rise to the rapid and massive production of ROS, so that DMPO and TEMP were instantly oxidized in electron paramagnetic resonance (ESR) detection. Importantly, the carrier of porous O–CN, which acted as the electron transfer mediator, not only favors PMS adsorption via surface –OH, but also facilitates the conversion between different metal species. As a result, the CuFe 2 O 4 @O–CN/PMS system can remove 99.1% BPA and achieve 52.6% mineralization under optimized conditions. Thus, this study not only sheds light on the tailored design of heterogeneous catalyst for organics removal and elucidates the interfacial catalytic mechanisms for PMS activation .
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Datong Wu,Cong Ma,Ting Wan,Pengfen Zhu,Yong Kong
In most cases, the recognition efficiency of chiral electroanalysis relies on the chiral carbon center, whereas its medium is just used to transfer the electron. Differently, in this study, an ionic helical polymer with right- or left-handed configuration was prepared via an acid-base interaction between the ionic polymer and enantiopure 1,2-diphenylethane-1,2-diamine. The structure and absolute handedness of the helical polymers were well characterized by the circular dichroism spectrum, gel permeation chromatography , and Fourier transform infrared spectroscopy . The pyridinium moiety of the helical polymer contributes to the electron transfer when it was directly modified on the surface of the glassy carbon electrode as an electrochemical enantioselector for chiral electroanalysis. Results indicated that different configurations of amino acids can be recognized in the responses of different electric signals, namely peak current or peak potential. The peak current ratio between L- and d -isomer can be up to 7.5 and 23.2 for tryptophan and tyrosine , respectively. Most importantly, the reversal of the electric signal can be observed toward L- and d -isomer under the different testing conditions such as pH and metal ion content, because of two stereogenic centers (the chiral inducer and the stereogenic axis) in the helical polymer. Overall, we believe that the present study offers a great promise for the synthesis and application of the ionic helical polymers accompanying with multiple stereogenic centers.
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RENEWABLE ENERGY
·
2022
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IF 8.634
Liu Yang,Mengyao Guo,Yiwen Qian,Deliang Xu,Mortaza Gholizadeh,Karnowo,Hong Zhang,Xun Hu,Shu Zhang
This study aims to examine the capability of nascent glucose-derived char on absorbing and solidifying N-containing gas species from the pyrolysis of fiberboard . A specially designed double-bed quartz reactor was used to carry out the experimental work. The N chemical forms and carbon structure in chars were characterized using XPS and Raman techniques, respectively. The results clearly indicate that the glucose char could effectively retain N-containing volatiles, especially at low temperatures due to its high content in O functional groups. The occurring forms of N inherently existing in fiberboard-derived char showed different transforming patterns from those in the glucose chars where the N compounds (e.g., amide-N) were externally obtained by intensive volatile-char interactions. The in-situ DRIFT analysis showed that the amino groups released from fiberboard may react with the aldehyde groups containing in the glucose char and form N-doped aromatic rings . Additionally, although the increase in carrier gas (thus decreasing the volatile-char interaction time) did not affect the N retention, the carbon structure revealed by Raman analysis dramatically altered.
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危险品化学品经营许可证（带存储）