METHODS
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2025
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IF 4.3
Wensi Li,Zhichao Tang,Xiang Zhu,Beijuan Wang,Xiuqin Zhang,Ze Ji,Suhang Cheng
In this work, an electrochemiluminescence (ECL) biosensor integrating T7 RNA polymerase amplification and CRISPR/Cas13a-mediated signal enhancement was developed for the ultrasensitive detection of matrix metalloproteinase-2 (MMP-2), a key biomarker associated with chronic inflammatory diseases such as COPD. A peptide nucleic acid (PNA) probe was designed to respond specifically to MMP-2 cleavage, enabling the release of DNA templates for subsequent T7 RNA polymerase-driven transcription amplification. The generated RNA triggers the collateral cleavage activity of CRISPR/Cas13a, resulting in a significant amplification of the ECL signal. The biosensor’s surface was constructed using a AuNPs/Ti 3 C 2 Tx/Ru(II)-PEI nanocomposite, which enhanced signal transduction and stability. Under optimized conditions, the proposed biosensor achieved a detection limit as low as 62.05 fM, demonstrating superior sensitivity compared to conventional methods, as summarized in Table 1 . The platform also exhibited excellent specificity and anti-interference capability, ensuring reliable detection of MMP-2 in complex biological samples. This study provides a simple yet highly efficient strategy for enzymatic biomarker detection, offering great potential for clinical applications in early disease diagnosis and monitoring.
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Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Feiyan He,Jilai Gong,Juan Li,Jun Zhao,Huaiyang Zhou,Yewei Wang,Yanzhao Hu
Nanofiltration (NF) membranes are part of the promising technologies for treating antibiotic wastewater. However, current NF membranes are constrained by the trade-off between the water permeability and selectivity. To address this challenge, a (2-Hydroxypropyl)-β-cyclodextrin (OH-β-CD) incorporated into nitrogen-doped quantum dots (N-GQDs) aqueous phase to form the membrane (N-CD 0.1 ). The integration of the OH-β-CD manipulated the structural properties of selective layer and improved the water permeability. This was attributed to the fact that OH-β-CD not only provided an additional priority water channel, but also regulated the interfacial polymerization process of the membrane by slowing down the diffusion rate of N-GQDs. In addition, the hydrophilicity and electronegativity of the membrane were optimized by OH-β-CD. The results showed that the N-CD 0.1 membrane achieved a high antibiotic rejection (Ciprofloxacin: 98.28 %, Norfloxacin: 97.9 % and Ofloxacin: 97.46 %). The N-CD 0.1 membrane had a remarkable water permeability of 15.82 L⋅m −2 ⋅h −1 ⋅bar −1 , which was 2.05-fold that of the N-GQDs membrane. Furthermore, the N-CD 0.1 membrane exhibited good high-pressure stability. When the operating pressure was 3 ∼ 7 bar, the N-CD 0.1 membrane still had a high Ciprofloxacin rejection (> 97 %). Therefore, by the addition of OH-β-CD, the water permeability of NF membrane could be further improved almost without sacrificing the rejection of Ciprofloxacin, which provided a green and efficient strategy for the fabrication of high-performance NF membranes.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
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IF 6.1
Yang Zhang,Mingcong Chen,Krishna Baral,Cheng Chen,Yang Meng,Manling Zhang,Marady Hun,Hanyong Zhang,Mengchang Xu,Mingyi Zhao
With the extensive use of microplastics (MPs) and its plasticizer, more and more research focus on their combined effects on health risk. In this study, polystyrene (PS) and di(2-ethylhexyl) phthalate (DEHP) were selected as target contaminants, and zebrafish embryos were employed as a biological model to investigate the potential toxicity of co-exposure to microplastics and plastic additives on embryonic and cardiac development. These findings indicated that the simultaneous exposure to PS and DEHP markedly diminished the heart rate, survival rate, and impeded the hatching process. The combined exposure group exhibited elevated levels of oxidative stress and an increased number of apoptotic cells. Fluorescence photography of zebrafish embryos revealed an increase in the distance of SV-BA in combined exposure. Transcriptomics analysis revealed a reduction in the expression of yeats4 , a gene involved in regulating DNA damage response and cell death. Metabolomics analysis revealed that amino acid metabolism plays a critical role in the toxic effects of combined PS and DEHP exposure on zebrafish larvae, while the Apelin signaling pathway, vascular smooth muscle contraction, and calcium ion signaling pathway may be key pathways involved in the cardiac toxic effects of PS and DEHP co-exposure in zebrafish larvae.
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Journal of Future Foods
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2025
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IF 7.2
Pengfei Dong,Shuang Wu,Youfa Wang,Ning Yu,Vijaya Raghavan,Jin Wang
Sesame allergy has emerged as a public health issue due to its persistent occurrence, severe symptomatic manifestations, and widespread incidence globally. Among sesame allergens, 2S albumins (Ses i 2) is one of the widely recognized allergens, and its precise detection is urgently needed to prevent sesame-related allergies. In this work, a composite structure was designed and synthesized by combining metal ion self-assembled metal organic frameworks (MOFs) and imine constructed covalent organic frameworks (COFs) (represented by MOF@COF), which constructing a high-sensitivity sensing system for identification and detection of Ses i 2. A novel hybrid material, comprising a strategically assembled combination of UIO-66-NH 2 and TA-COF, was crafted to yield a functionalized UIO-66-NH 2 @TA-COF composite, demonstrating remarkable electrochemical performance. As a result, a significant quantity of aptamer strands can adhere to the UIO-66-NH 2 @TA-COF surface resulting from robust π-π stacking interactions. With the change in the relative concentration of Ses i 2 from 5 to 650 nM, the UIO-66-NH 2 @TA-COF sensor exhibited a low detection limit of 0.13 nM, which demonstrates its extremely high sensitivity. In addition, the UIO-66-NH 2 @TA-COF sensor exhibited excellent reproducibility, stability and specific recognition ability to Ses i 2. Therefore, this innovative sensor exhibits great potential for real-world applications in food safety.
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BIOSENSORS & BIOELECTRONICS
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2025
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IF 10.5
Hang Ao,Jinshan Xiong,Wencheng Xiao,Wenrui Hu,Jie Wu,Huangxian Ju
Owing to the continuous mutations of the coronavirus, the sensitive and accurate detection of the SARS-CoV-2 nucleocapsid protein is still necessary in clinical diagnosis. Aim to the challenge of sensitivity in chemiluminescence (CL) imaging detection and the need for high-throughput clinical screening, this work used imidazole (Im) as an enhancer for hemin-DNA/luminol/H 2 O 2 CL system to propose a CL imaging method for SARS-CoV-2 nucleocapsid protein detection. In this system Im could reduce the hemin-DNA catalyzed oxidation energy of luminol by H 2 O 2 to promote the CL emission through the accelerated breaking of O-O bond of dianionic cyclic peroxide. The enhancement mechanism was demonstrated with theoretical calculations and several characterization techniques. By combining the proximity-induced initiator release with immunological recognition, an initiator-triggered DNA nanomachine was designed to achieve the release of abundant primers for activating hemin-DNA switch. This signal amplification strategy led to strong CL emission for highly sensitive imaging detection of the target. The Im-enhanced hemin-DNA/luminol/H 2 O 2 CL imaging assay exhibited a linear detection range over 4 orders of magnitude with a detection limit down to 2.75 pg/mL for SARS-CoV-2 nucleocapsid protein, exhibiting promising potential of both Im sensitization and the proposed DNA nanomachine to improve the sensitivity of high-throughput CL biosensing for the early diagnosis and clinical screening of the coronavirus and other diseases.
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APPLIED CATALYSIS A-GENERAL
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2025
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IF 4.8
Ruxing Zhao,Qingrun Meng,Guanchao Wang,Lin Xu,Weifeng Chu,Junjie Li
Intermolecular dehydration of 2-propanol (IPA) to di-isopropyl ether (DIPE) serves as an important process to produce the valuable chemical, however, developing advanced solid acid catalysts with high DIPE selectivity faces the great challenge of inhibiting the intramolecular dehydration reaction. In this context, we developed a highly selective intermolecular dehydration MFI nanosheet catalyst by synergistically modifying its diffusion and acid properties. We found that the DIPE selectivity over nanosheet ZSM-5 catalysts were obviously higher than that over the conventional ZSM-5 catalyst, benefited from the enhanced diffusion property over nanosheet catalysts that inhibited the further conversion of DIPE. However, the DIPE selectivity were still lower than 70 % over those catalysts attributed to their high acid strength that facilitated the intramolecular dehydration reaction. Thus, various heteroatoms (Fe, Ga, and B) substituted MFI zeolite nanosheet catalysts were prepared and employed in the dehydration reaction, Fe-MFI with a moderate acid strength exhibited the highest DIPE selectivity among those catalysts. Besides, the effects of acid amounts (Si/Fe ratio) and diffusion properties (thickness of b-axis) on the intermolecular dehydration selectivity and activities over nanosheet Fe-MFI catalysts were systematically investigated. An optimal Fe-MFI nanosheet catalyst with the b-axis thickness of ∼20 nm and Si/Fe ratio of ∼40 presented the stable high DIPE selectivity of ∼91 % in 360 h of time on stream, meanwhile the IPA conversions kept almost unchanged.
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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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2025
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IF 3.7
Qian Zhu,Kangning Wei,Kaihua Pan,Gang Hu,Weihao Zhu,Yanni Huang,Changchang Wang,Qian Li,Mingliang Zhang,Jiguo Qiu,Qing Hong
Isoprocarb (IPC), a widely used carbamate insecticide, causes environmental contamination and poses risks to humans and ecosystems. Rhodococcus sp. D-6, capable of utilizing IPC as the sole growth substrate, was isolated by our lab. Strain D-6 initiates IPC degradation through ester bond hydrolysis by the hydrolase IpcH, yielding 2-isopropylphenol (IPP). In this study, the catabolic pathway of IPP in strain D-6 was elucidated, and the ipp gene cluster responsible for its degradation was predicted by transcriptomic analysis. Key genes, including a novel two-component IPP monooxygenase gene ippA1A2 and a 2-isopropylhydroquinone dioxygenase gene ippB, are responsible for the successive conversion of IPP, resulting in the ring cleavage of IPP. IppA1, a group D flavin-dependent monooxygenase, along with its reductase component IppA2, hydroxylates IPP to 2-isopropylhydroquinone using reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) as cofactors. The optimal molar ratio of IppA1 to IppA2 was 3:1. The apparent Km and kcat values of IppA1 for IPP were 60.52 ± 1.27 µM and 70.07 ± 0.85 s−1, respectively. IppB, an extradiol dioxygenase, catalyzes 2-isopropylhydroquinone ring cleavage to generate 2-isopropyl-4-hydroxymuconic semialdehyde. The apparent Km and kcat values of IppB for 2-isopropylhydroquinone were 29.07 ± 1.54 μM and 147.65 ± 1.92 s−1, respectively. This study provides critical insights into the molecular mechanisms of IPP degradation, contributing to a comprehensive understanding of IPC degradation.
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JOURNAL OF MOLECULAR STRUCTURE
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2024
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IF 4
Meng Ye,Hongying Hou,Xianxi Liu,Zhaowei Sun,Xiaohua Yu,Ju Rong
With the development of the consumer society, low cost and high performance supercapacitors are being pursued. To realize the practical supercapacitors, developing the cheap and highly capacitive electrode is the most essential. However, it is a harsh challenge to achieve this target based on single-component electrode. Herein, a series of inexpensive ternary-metal-sulfides, 2MnS/Cu 2 S/ x ZnS/C ( x = 0, 0.5, 1, 2, 3) are synthesized by hydrothermal reaction and the subsequent calcination with expired manganese/copper gluconates as the raw materials for the electrode in the pseudocapacitors. Furthermore, the effect of ZnS's content on the electrochemical properties of the ternary composites is also investigated. Specially, the optimal 2MnS/Cu 2 S/xZnS/C( x = 1) can output a specific capacitance of 1608 F/g at 1 A/g in the three-electrode system, while the 2MnS/Cu 2 S/xZnS/C||AC( x = 1) asymmetric supercapacitor can deliver a specific capacitance of 194 F/g at 1 A/g with the desirable energy density (800 W/kg) and power density (70.9 Wh/kg), higher than those of other samples. Especially, even after 2000 cycles at 5.0 A/g, the 2MnS/Cu 2 S/xZnS/C||AC( x = 1) asymmetric supercapacitor also delivers the reversible specific capacitance of 103 F/g with high capacitance retention of 97.0 %, indicating high cycling stability. The outstanding performances of the optimal 2MnS/Cu 2 S/xZnS/C( x = 1) may be attributed to the bridge-effect of ZnS component, which makes up for the low conductivity of MnS component and the low capacitance of Cu 2 S component, thus achieving the best synergistic effect of ternary metal sulfide 2MnS/Cu 2 S/xZnS/C( x = 1) .
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Materials Science and Engineering B-Advanced Functional Solid-State Materials
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2024
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IF 3.9
Congzhi Hu,Yuye Li,Tingting Du,Youyong Pang,Yongping Liu,Botian Liu,Ling Li,Huidan Lu,Bin Huang
In this paper, a new supramolecular Z-scheme heterojunction photocatalyst, designated as Ag/Ag 3 PO 4 /Perylene diimide organic molecules after secondary acid-base self-assembly (PDI-2), was synthesized through precipitation and photoreduction techniques. Ag/Ag 3 PO 4 /60 %PDI-2 (APP-6) exhibited the best photocatalytic activity due to the synergistic effect of Z-scheme heterojunction and surface plasmon resonance (SPR) effect of Ag nanoparticles (Ag NPs). Under visible light (λ > 420 nm), the degradation efficiency of APP-6 for Rhodamine B (RhB) and Bisphenol A (BPA) was 99.7 % and 90.7 %, respectively. Additionally, PDI-2 was transformed into smaller nanoclusters, which increased the specific surface area of the photocatalyst, and reduced the charge transport distance. Moreover, APP-6 exhibited the smallest electrochemical impedance, the largest photocurrent, the smallest fluorescence lifetime. Free radical trapping experiments showed that •O 2 − was the main reactive group and a possible photocatalytic mechanism was proposed by combining the Ultraviolet–visible diffuse reflectance spectrophotometer (UV–vis DRS) and the Mott-Schottky plots.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Shuang Qi,Tingwei Zhang,Chaofeng Zhang,Bo Jiang,Caoxing Huang,Qiang Yong,Yongcan Jin
As the most important phenolic biopolymer in nature, lignin shows promising application potentialities in various bioactivities in vivo and in vitro, mainly including antioxidant, anti-inflammatory, hypolipidemic, and antidiabetic control. In this work, several carbon-based solid acids were synthesized to catalyze the fragmentation of organosolv lignin (OL). The generated lignin fragments, with controllable molecular weight and functional groups, were further evaluated for their application in the prevention and treatment of type 2 diabetes mellitus (T2DM). The results suggested that the urea-doped catalyst (SUPC) showed a more excellent catalytic performance in producing diethyl ether insoluble lignin (DEIL) and diethyl ether soluble lignin (DESL). In addition, the lignin fragments have a good therapeutic effect on the cell model of T2DM. Compared with the insulin resistance model, DEIL obtained by catalytic depolymerization of OL with SUPC could improve the glucose consumption of insulin-resistant cells. Moreover, low-concentration samples (50 μg/mL) can promote glucose consumption (19.7 mM) more than the traditional drug rosiglitazone (17.5 mM). This work demonstrates the prospect of depolymerized lignin for the prevention and treatment of T2DM and provides a new application field for lignin degradation products.
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JOURNAL OF MEDICINAL CHEMISTRY
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2024
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IF 6.8
Wen Shuai,Huan Xiao,Panpan Yang,Yiwen Zhang,Faqian Bu,Yongya Wu,Qiu Sun,Guan Wang,Liang Ouyang
The RAS-RAF-MEK-ERK signaling cascade is abnormally activated in various tumors, playing a crucial role in mediating tumor progression. As the key component at the terminal stage of this cascade, ERK1/2 emerges as a potential antitumor target and offers a promising therapeutic strategy for tumors harboring BRAF or RAS mutations. Here, we identified 36c with a (thiophen-3-yl)aminopyrimidine scaffold as a potent ERK1/2 inhibitor through structure-guided optimization for hit 18. In preclinical studies, 36c showed powerful ERK1/2 inhibitory activities (ERK1/2 IC50 = 0.11/0.08 nM) and potent antitumor efficacy both in vitro and in vivo against triple-negative breast cancer and colorectal cancer models harboring BRAF and RAS mutations. 36c could directly inhibit ERK1/2, significantly block the phosphorylation expression of their downstream substrates p90RSK and c-Myc, and induce cell apoptosis and incomplete autophagy-related cell death. Taken together, this work provides a promising ERK1/2 lead compound for multiple tumor-treatment drug discovery.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
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IF 5.2
Zixuan Wang,Yingxiao Sun,Peijie Dong,Jinhu Wang,Lijie Wang,Aili Zhao,Guangmin Qu,Hang Li,Kannapathirage Dilini Maheshika Gunarathne,Wen Zhang,Yao Chen,Xin Meng
Trauma healing is the process of healing after the body has been subjected to an external force and the skin and other tissues have become dissected or defective, showing the synergistic effect of various processes. Therefore, the investigation of innovative wound dressings has significant research and clinical implications. In this study, we constructed a zinc based metal–organic framework (MOF) and loaded with antimicrobial peptide LL37 to prepare LL37@ZPF-2 (ZPF = zeolite pyrimidine backbone), which was subsequently integrated with Poloxamer 407 to fabricate LL37@ZPF-2 thermosensitive hydrogel. Our study showed that in-situ packaging method can achieve encapsulation rate of 98 % and 15 % of drug loading for LL37. LL37@ZPF-2 demonstrated a higher inhibitory potency against S.aureus compared to E.coli . The Poloxamer 407-gel exhibits thermo-responsive sol–to–gel phase transition behaviors with a phase transition temperature (Tsol/gel) of ∼ 28.01℃, making it an appropriate material for wound healing. The composite hydrogel has excellent biocompatibility and hemocompatibility. A full-thickness skin defect model was built to confirm that LL37@ZPF-2 thermosensitive hydrogel dressing could inhibit bacterial growth, reduce the risk of wound infection, and stimulate angiogenesis and collagen deposition, resulting in a wound healing rate of 94.4 % on day 7 and complete healing on day 10. Our findings demonstrate that the novel thermosensitive LL37@ZPF-2 hydrogel confers good antibacterial activity, promoting cell migration and infected-wound healing properties, providing a promising platform for wound healing.
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