ACTA PHARMACOLOGICA SINICA
·
2023
·
IF 8.2
Li Yi-jie,Wu Rui-yu,Liu Run-ping,Wu Kai-yi,Ding Ming-ning,Sun Rong,Gu Yi-qing,Zhou Fei,Wu Jian-zhi,Zheng Qi,Duan Shu-ni,Li Rong-rong,Zhang Yin-hao,Li Fang-hong,Li Xiaojiaoyang
Obesity contributes to the progression of various chronic diseases, and shortens life expectancy. With abundant mitochondria, brown adipose tissue (BAT) dissipates energy through heat to limit weight gain and metabolic dysfunction in obesity. Our previous studies have shown that aurantio-obtusin (AO), a bioactive ingredient in Chinese traditional medicine Cassiae semen significantly improves hepatic lipid metabolism in a steatotic mouse model. In the current study we investigated the effects of AO on lipid metabolism in the BAT of diet-induced obesity mice and in oleic acid and palmitic acid (OAPA)-stimulated primary mature BAT adipocytes. Obese mice were established by feeding a HFHS diet for 4 weeks, and then administered AO (10 mg/kg, i.g.) for another 4 weeks. We showed that AO administration significantly increased the weight of BAT and accelerated energy expenditure to protect the weight increase in the obese mice. Using RNA sequencing and molecular biology analysis we found that AO significantly enhanced mitochondrial metabolism and UCP1 expression by activating PPARα both in vivo and in vitro in the primary BAT adipocytes. Interestingly, AO administration did not improve metabolic dysfunction in the liver and white adipose tissue of obese mice after interscapular BAT excision. We demonstrated that low temperature, a trigger of BAT thermogenesis, was not a decisive factor for AO to stimulate the growth and activation of BATs. This study uncovers a regulatory network of AO in activating BAT-dependent lipid consumption and brings up a new avenue for the pharmaceutical intervention in obesity and related comorbidities.
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高影响因子
Biomaterials Research
·
2023
·Vol. 27
·期 1
·1-22
·
IF 11.3
Shen Shuilin,Zhang Zimeng,Huang Haixiao,Yang Jing,Tao Xinyue,Meng Zhengjie,Ren Hao,Li Xueming
Background Immunogenic cell death (ICD) induced by different cancer treatments has been widely evaluated to recruit immune cells and trigger the specific antitumor immunity. However, cancer associated fibroblasts (CAFs) can hinder the invasion of immune cells and polarize the recruited monocytes to M2-type macrophages, which greatly restrict the efficacy of immunotherapy (IT). Methods In this study, an injectable hydrogel induced by copper (Cu) has been designed to contain antibody of PD-L1 and nitric oxide (NO) donor. The therapeutic efficacy of hydrogel was studied in 4T1 cells and CAFs in vitro and 4T1 tumor-bearing mice in vivo. The immune effects on cytotoxic T lymphocytes, dendritic cells (DCs) and macrophages were analyzed by flow cytometry. Enzyme-linked immunosorbent assay, immunofluorescence and transcriptome analyses were also performed to evaluate the underlying mechanism. Results Due to the absorbance of Cu with the near-infrared laser irradiation, the injectable hydrogel exhibits persistent photothermal effect to kill cancer cells. In addition, the Cu of hydrogel shows the Fenton-like reaction to produce reactive oxygen species as chemodynamic therapy, thereby enhancing cancer treatment and amplifying ICD. More interestingly, we have found that the released NO can significantly increase depletion of CAFs and reduce the proportion of M2-type macrophages in vitro. Furthermore, due to the amplify of ICD, injectable hydrogel can effectively increase the infiltration of immune cells and reverse the immunosuppressive tumor microenvironment (TME) by regulating CAFs to enhance the therapeutic efficacy of anti-PD-L1 in vivo. Conclusions The ion induced self-assembled hydrogel with NO could enhance immunotherapy via amplifying ICD and regulating CAFs. It provides a novel strategy to provoke a robust antitumor immune response for clinical cancer immunotherapy. Graphical Abstract
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 30
·76157-76170
·
IF 5.8
Shao Shujing,Zhang Pengyu,Chen Yang,Zhao Xiaodan
Iron-based heterogeneous catalysts due to the environmental friendliness have been widely studied for activation of peracetic acid (PAA) for abatement of organic contaminants in the water and wastewater treatment. However, the slow reduction from Fe(III) to Fe(II) of the iron-based catalysts as the rate-limiting step results in the low PAA activation efficiency. With regard to the excellent electron-donating capability of the reductive sulfur species, sulfidized nanoscale zerovalent iron is proposed for PAA activation (simplified as the S-nZVI/PAA process) and the tetracycline (TC) abatement efficacy and mechanism of this process are elucidated. The optimal sulfidation ratio (S/Fe) of S-nZVI is 0.07, which exhibits superior performance in PAA activation for TC abatement with the efficiency of 80–100% in the pH range of 4.0–10.0. The radical quenching experiments and oxygen release measurements confirm that acetyl(per)oxygen radicals (CH 3 C(O)OO•) are the main radical contributing to TC abatement. The influence of sulfidation on the crystalline structure, hydrophobicity, corrosion potential, and electron transfer resistance of S-nZVI is evaluated. The main sulfur species on the S-nZVI surface are identified as ferrous sulfide (FeS) and ferrous disulfide (FeS 2 ). The analysis by X-ray photoelectron spectroscopy (XPS) and Fe(II) dissolution suggest that the reductive sulfur species can accelerate the conversion from Fe(III) to Fe(II). In summary, the S-nZVI/PAA process exhibits application prospects for the abatement of antibiotics in the aquatic environments. Graphical Abstract
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高影响因子
IONICS
·
2023
·Vol. 29
·期 7
·2947-2958
·
IF 2.8
Guan Pingping,Li Haobo,Zhang Xin,Shi Zhongning,Liu Aimin
Zinc is a commonly used corrosion-resistant coating for steel surface protection. In this study, the ethylene carbonate-zinc chloride (EC-ZnCl 2 ) electrolyte systems are selected for zinc electrodeposition, and their ionic structure and properties were studied. The corrosion resistance of the electrodeposited zinc coatings obtained from 4EC-ZnCl 2 , 3EC-ZnCl 2 , and 2EC-ZnCl 2 systems was analyzed. Raman spectroscopic analysis shows that these EC-ZnCl 2 systems contain [Zn 2 Cl 6 ] 2− species. The relationship between conductivity, viscosity, and density with the temperature of EC-ZnCl 2 systems was provided. Scanning electron microscopy (SEM) and potentiodynamic polarization curve (Tafel) tests showed that when the deposition was carried out at –1.0 V and –1.2 V (vs. Ag/AgCl) for two hours, a uniform zinc coating with good corrosion resistance could be obtained.
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高影响因子
JOURNAL OF POLYMER RESEARCH
·
2023
·Vol. 30
·期 6
·1-8
·
IF 2.8
Yu Dayang,Zhong Jiachun,Pu Zejun,Hou Hongbo,Li Xianyong,Zhu Rongli,Wang Xu,Wu Fang,Zheng Pan,Liu Jingyue
In this study, iso-sorbitol type polycarbonate (PIC) was synthesized with isosorbide (ISB) and diphenyl carbonate (DPC) as reactive monomers. The flexible co-monomers 1,4-butanediol (BDO) and 1,4-cyclohexane dimethanol (CHDM) were introduced to improve its property. Then, two iso-sorbitol type polycarbonate copolymers were obtained: Isosorbitol type polycarbonate copolymer (PIBC) containing 1,4-butanediol (BDO) and isosorbitol type polycarbonate copolymer (PICC) containing 1,4-cyclohexane dimethanol (CHDM). The results of 1 H-NMR and FT-IR demonstrated that the structures of PC, PIC, PIBC, and PICC synthesized by the experiment were consistent with the theoretical structures. The results of the characteristic viscosity test suggested that the copolymer with high relative molecular weight was successfully prepared. The results of DSC and TGA revealed that the introduction of a flexible copolymer increased the relative molecular weight of the copolymer, decreased the glass transition temperature, and exerted little effect on the thermal stability of the copolymer. The mechanical properties test results unveiled that PICC has good mechanical properties. Owing to its excellent mechanical and thermal properties, there is a broad application prospect of the PICC as a green polycarbonate material.
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高影响因子
Carbon Letters
·
2023
·
IF 4.5
Li Ronghua,Hu Xiucai,Shan Shiqiang,Li Yongbo,Cui Wenbin,Liu Lingling
Sulforaphane is a naturally occurring active substance found in vegetables that is known for its potential in preventing and treating cancer. This compound has demonstrated promising effects in inhibiting the growth of various types of cancer, including esophageal, lung, colon, breast, and liver cancer. However, its instability towards pH and heat limits its application in the medical and food industries. To address this challenge, novel drug delivery systems have been developed to improve the stability and efficacy of sulforaphane, making it a more suitable candidate for clinical use in cancer research. In this study, nanocomposite materials were prepared using multi-walled carbon nanotubes (MWCNTs) and chitosan (CS) as base materials, with polydopamine (PDA) acting as a bridge material. The synthesized composite materials were used as drug carriers for the release of sulforaphane. The results of the study showed that the drug loading increased with an increase in the concentration of sulforaphane, indicating that the nanocomposite materials were effective in delivering and releasing the drug. Moreover, a positive correlation was observed between the drug loading and the thickness of the PDA layer. These findings suggest that the use of MWCNTs, CS, and PDA in the development of drug delivery systems can enhance the stability and efficacy of sulforaphane, potentially leading to improved cancer treatment outcomes.
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Food and Bioprocess Technology
·
2023
·Vol. 16
·期 12
·3003-3018
·
IF 5.6
Cheng Shasha,Dong Xue,Wang Haitao,Song Yukun,Tan Mingqian
Carbon-based antibacterial materials have attracted considerable attention to preserve food and prolong the shelf life. In this study, four types of carbon dots (CDs) based on glucose and biogenic amines including spermine (Spe), putrescine (Put), spermidine (Spd) and histamine (His) were synthesized by microwave synthesis. The nanosized, amorphous and nearly spherical CDs had excellent water solubility. The four positive charge CDs containing carbon, nitrogen and oxygen elements could emit blue fluorescent. Among these CDs, Spe-CDs displayed the best antibacterial activity for S. aureus , and the values of minimum inhibitory concentration and minimum bactericidal concentration were 0.04 and 0.08 mg/mL, respectively. The exposure of Spe-CDs was able to disrupt the cell wall and membrane of S. aureus , which resulted in the shrinkage and disintegration of S. aureus observed in cell morphology. The excellent antibacterial activity of Spe-CDs was attributed to its smaller size, higher surface positive charge and higher ROS production, compared to Put-CDs, Spd-CDs and His-CDs. The preservation effect of Spe-CDs on sausage was explored during 4 °C storage, which could effectively inhibit the spoilage of sausage and prolong their shelf-life. The Spe-CDs might have the potential to be a food antibacterial preservation agent.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2023
·Vol. 148
·期 14
·7259-7267
·
IF 4.4
Tian Wenshuang,Xiao Yang,Zhou Beiting,Qin Guangzhao,Zheng Xiong
As a new type of green solvent, deep eutectic solvents (DESs) are regarded as substitutes for ionic liquids in various applications, so the study of thermophysical properties of DESs is of great importance for their applications. In this work, DESs containing triethylene glycol as hydrogen bonding donor and choline chloride (ChCl) as hydrogen bond acceptor were synthesized in a series of molar ratios, and the thermal conductivity, viscosity, and density were investigated systematically. The addition of ChCl suppresses the thermal conductivity of DESs, but increases the viscosity and density, and the mechanism of the dependence of the thermophysical properties on the molar fraction is analyzed. The increase in the temperature always causes a decrease in the viscosity and density, while the thermal conductivity and temperature show a parabola dependence. Besides, the increase in the pressure results in the enhancement of thermal conductivity. Moreover, the thermophysical properties of DESs are fitted into correlations, and the AADs and MDs from fitted values and experimental data are 0.39% and 2.14% for thermal conductivity, 1.34% and 2.49% for viscosity, and 0.04% and 0.11% for density, respectively.
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Biomass Conversion and Biorefinery
·
2023
·
IF 4
Zhu Linchao,Wang Lin,Yuan Peiqing,Xu Xinru,Yang Jingyi
Volatile fatty acids (VFAs) such as acetic acid have a wide range of applications with high demand. Since acetic acid is well soluble in water, treating its wastewater presents a technical challenge. In this work, new hydrophobic deep eutectic solvents (DESs) were synthesized by using methyltrioctylammonium chloride (TOMAC) as hydrogen bond acceptor (HBA) and acetic acid as hydrogen bond donor (HBD). The properties of DESs were characterized by DSC, TG, and FT-IR. As HBA, TOMAC was used for in situ generation of DES to extract acetic acid from aqueous solution. The extraction efficiency reached 65.28% when TOMAC/acetic acid was 7:3 (molar ratio), which is better than that of the traditional acetic acid extractants, such as tributyl phosphate (60.46%). When metal ions such as Ca 2+ , Mg 2+ , and Fe 3+ coexist in solution, the salt precipitation effect not only prevents the emulsification phenomenon but also improves the acetic acid extraction effect. For volatile fatty acids such as propionic acid, butyric acid, and valeric acid, the extraction efficiency of TOMAC was good, reaching 84.96%, 92.04%, and 95.81%, respectively. The method of using TOMAC to extract acetic acid from aqueous solutions by forming DES in situ provides a new way for the treatment of VFAs wastewater. Graphical Abstract
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 18
·7571-7582
·
IF 4.5
Yang Yang,Lu Jie,Ma Zhichun,Zhao Yi,Wang Shuang,Ge Kai,Yang Yongfang
Two dimensional (2D) CuTCPP/B–TiO 2 composites were successfully synthesized by addition of B–TiO 2 NPs into the system for preparation of 2D CuTCPP nanosheets under hydrothermal condition. 2D CuTCPP nanosheets with large specific surface area can not only act as supportor to decrease the aggregation of B–TiO 2 and improve the adsorption capacity of methylene blue (MB) but also provide more transfer pathway to effectively facilitate interfacial charge transfer and separation of photogenerated electrons and holes, thus leading to a superior efficiency for photodegradation of MB. The photocatalytic degradation rate constant of 2D CuTCPP/B–TiO 2 composites is 0.0486 min −1 , which is about 15.2, 5.92 and 4.23 times that of P25 (k = 0.0032 min −1 ), 2D CuTCPP nanosheets ((k = 0.0082 min −1 ) and B–TiO 2 NPs (k = 0.0115 min −1 ). This study demonstrates that the combination of B–TiO 2 NPs and 2D CuTCPP is a viable strategy to improve the photocatalytic efficiency for degradation of MB.
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Cancer Nanotechnology
·
2023
·Vol. 14
·期 1
·1-16
·
IF 5.7
Bai Zhiqiang,Zhao Lu,Feng Haidi,Xin Zhihui,Wang Chaoyu,Liu Zhixiong,Tian Maozhong,Zhang Haifei,Bai Yunfeng,Feng Feng
Background Ti 3 C 2 is a type of transition metal carbides and nitrides (MXenes) with high light-to-heat conversion efficiency property, which has been widely used in cancer treatment recently. In fact, active targeting delivery of MXenes nanomaterials with targeting molecule could enhance the therapeutic efficacy. However, targeted therapy of MXenes has not been further studied in the past. Aptamers (Apt) with excellent affinity and high specificity properties have been widely used as targeting tools. Predictably, the incorporation of Apt into Ti 3 C 2 nanomaterials will offer an unprecedented opportunity in the research fields of cancer targeted therapy. Results Transmembrane glycoprotein mucin 1 (MUC1) is overexpressed on the surface of MCF-7 cells, and MUC1 Apt (Apt-M) could target MCF-7 cells with high affinity and specificity. Here, a smart targeting nanotherapeutic system Ti 3 C 2 /Apt-M was fabricated, which could specifically recognize and enter in MCF-7 cells. Benefitting from the desirable targeted performance of Apt-M, MCF-7 cells completed the ingestion process of Ti 3 C 2 /Apt-Mf nanosheets within 4 h, and Apt-M facilitated the entry of the Ti 3 C 2 /Apt-Mf nanosheets into MCF-7 cells. Besides, Ti 3 C 2 /Apt-M nanosheets exhibited the potential as an outstanding photothermal agent (PTA) because of the photothermal performance inherited from wrapped Ti 3 C 2 nanosheets. As demonstrated, upon 808 nm laser irradiation, the Ti 3 C 2 /Apt-M nanotherapeutic system displayed a satisfactory antitumor effect by targeted photothermal therapy both in vitro and in vivo. Conclusion This study provides a new idea for the development of MXenes nanotherapeutic system with high active targeting performance. Graphical Abstract
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Microbial Cell Factories
·
2023
·Vol. 22
·期 1
·1-11
·
IF 6.4
Lin Yu,Chen Wen-wen,Ding Bo,Guo Man,Liang Meng,Pang Hao,Wei Yu-tuo,Huang Ri-bo,Du Li-qin
Background Icaritin is an aglycone of flavonoid glycosides from Herba Epimedii. It has good performance in the treatment of hepatocellular carcinoma in clinical trials. However, the natural icaritin content of Herba Epimedii is very low. At present, the icaritin is mainly prepared from flavonoid glycosides by α-L-rhamnosidases and β-glucosidases in two-step catalysis process. However, one-pot icaritin production required reported enzymes to be immobilized or bifunctional enzymes to hydrolyze substrate with long reaction time, which caused complicated operations and high costs. To improve the production efficiency and reduce costs, we explored α-L-rhamnosidase SPRHA2 and β-glucosidase PBGL to directly hydrolyze icariin to icaritin in one-pot, and developed the whole-cell catalytic method for efficient icaritin production.Results The SPRHA2 and PBGL were expressed in Escherichia coli, respectively. One-pot production of icaritin was achieved by co-catalysis of SPRHA2 and PBGL. Moreover, whole-cell catalysis was developed for icariin hydrolysis. The mixture of SPRHA2 cells and PBGL cells transformed 200 g/L icariin into 103.69 g/L icaritin (yield 95.23%) in 4 h in whole-cell catalysis under the optimized reaction conditions. In order to further increase the production efficiency and simplify operations, we also constructed recombinant E. coli strains that co-expressed SPRHA2 and PBGL. Crude icariin extracts were also efficiently hydrolyzed by the whole-cell catalytic system.Conclusion sCompared to previous reports on icaritin production, in this study, whole-cell catalysis showed higher production efficiency of icaritin. This study provides promising approach for industrial production of icaritin in the future.
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高影响因子
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