Food and Bioprocess Technology
·
2022
·Vol. 15
·期 1
·190-202
·
IF 5.581
Wang Liling,Wang Yanbin,Qin Yuchuan,Liu Bentong,Zhou Yifeng
This study aimed to develop the extraction method of protein from edible oil for rapid detection. Firstly, aqueous biphasic systems (ABS) based on six hydrophilic ionic liquids (ILs) and three salts were developed and the phase diagram was drawn by turbidimetric point method. The binodal curves were fitted to the Merchuk equation. On this basis, the ABS composed of IL and salt were applied to extract protein from edible oil. The type of IL or salt, IL concentration, salt concentration, oil mass, extraction pH, and temperature on the extraction efficiency of protein from oil were investigated. The results showed that the optimum conditions for the extraction of protein from edible oil with ABS were as follows: 50% (w/v) K 3 PO 4 , 20% (w/v) [Bmim]Cl at 35 ℃, and pH 9.0. Under the optimal conditions, the protein extraction efficiency was almost 100%. Also, the extraction mechanism was studied and the main driving factors of protein extraction may be the hydrophobicity, electrostatic interaction, and salting-out between molecules. Finally, the method was used to detect the commercial edible oils from different sources. The results showed that the ABS could also be used to extract protein from other edible oils. In conclusion, the IL-based ABS method is simple and rapid for protein extraction from edible oil, and will highlight novel possibilities in the large-scale separation and purification of protein from oily solution.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-16
·
IF 9.429
Wang Dan,Chen Ling,Li Chengbi,Long Quanxin,Yang Qing,Huang Ailong,Tang Hua
Background Currently, there are no curative drugs for hepatitis B virus (HBV). Complete elimination of HBV covalently closed circular DNA (cccDNA) is key to the complete cure of hepatitis B virus infection. The CRISPR/Cas9 system can directly destroy HBV cccDNA. However, a CRISPR/Cas9 delivery system with low immunogenicity and high efficiency has not yet been established. Moreover, effective implementation of precise remote spatiotemporal operations in CRISPR/Cas9 is a major limitation.Results In this work, we designed NIR-responsive biomimetic nanoparticles (UCNPs-Cas9@CM), which could effectively deliver Cas9 RNP to achieve effective genome editing for HBV therapy. HBsAg, HBeAg, HBV pgRNA and HBV DNA along with cccDNA in HBV-infected cells were found to be inhibited. These findings were confirmed in HBV-Tg mice, which did not exhibit significant cytotoxicity and minimal off-target DNA damage.Conclusion sThe UCNPs-based biomimetic nanoplatforms achieved the inhibition of HBV replication via CRISPR therapy and it is a potential system for efficient treatment of human HBV diseases.Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 4
·1107-1117
·
IF 3.498
Wei Liulin,Liu Jie,Liu Qian,Chen Xiaomei,Li Zhiqiang,Xu Yidong,Gao Xueyun,Lu Xiaoxiao,Guo Zhian,Zhao Jingchan
In this study, a convenient and sensitive method for the determination of phthalate esters (PAEs) in plastic bottled water was proposed by on-line in-tube solid-phase microextraction (IT-SPME) coupled with high-performance liquid chromatography (HPLC). A hybrid monolithic column was synthesized with octamethacrylate substituted polyhedral oligomeric silsesquioxane (POSS-MA) as cross-linker and N-butyl methacrylate (BMA) as functional monomer, which was utilized as the extraction phase of IT-SPME. A number of important extraction parameters were systematically optimized, such as sampling flow rate, desorption solvent flow rate and volume, sample volume, and sample pH value. Under the optimal experimental conditions, the linear ranges of five PAEs were in the range of 0.1–60 ng mL −1 . The limits of detection were between 0.052 and 0.10 ng mL −1 , and the limits of quantification were less than 0.33 ng mL −1 . In addition, the intra-day and inter-day relative standard deviations of the method were 0.98–3.0% and 2.0–4.3%, respectively. The EFs of the method for five PAEs were more than 538. The proposed method was also successfully applied to analyze trace PAEs in real samples with good recoveries. The recoveries of this method ranged from 84.6 to 111.6% and the relative standard deviations were less than 4.5%. The extraction recoveries of the prepared hybrid monolithic column of the target PAEs did not decrease significantly within 60 cycles. The results showed that the on-line IT-SPME-HPLC method was sensitive, highly efficient, and operationally convenient.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 4
·1651-1662
·
IF 4.478
Zhang Wei,Zhang Yu,Liu Xin,Zhang Yue,Liu Yibing,Wang Wei,Su Rui,Sun Ying,Huang Yibing,Song Daqian,Wu Yanhua,Wang Xinghua
A sensing platform with both ratiometric fluorescence and colorimetric responses towards copper(II) ions (Cu 2+ ) and D-penicillamine (D-pen) was constructed based on carbon dots (CDs). o -Phenylenediamine (OPD) was employed as a chromogenic development reagent for reaction with Cu 2+ to generate the oxidation product 2,3-diaminophenazine (oxOPD), which not only emits green fluorescence at 555 nm, but also quenches the blue fluorescence of CDs at 443 nm via the inner filter effect (IFE) and Förster resonance energy transfer (FRET). Additionally, oxOPD exhibits obvious absorption at 420 nm. Since the intense chelation affinity of D-pen to Cu 2+ greatly inhibits the oxidation of OPD, the intensity ratio of fluorescence at 443 nm to that at 555 nm ( F 443 / F 555 ) and the absorbance at 420 nm ( A 420 ) were conveniently employed as spectral response signals to represent the amount of D-pen introduced into the testing system. This dual-signal sensing platform exhibits excellent selectivity and sensitivity towards both Cu 2+ and D-pen, with low detection limits of 0.019 μM and 0.092 μM, respectively. In addition, the low cytotoxicity of the testing reagents involved in the proposed sensing platform facilitates its application for live cell imaging. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 6
·2065-2078
·
IF 4.478
Ou Panpan,Wu Jiawen,Lin Yu,Tan Xuecai,Wu Yeyu,Chen Zhifan,Wei Fucun,Huang Kejing
Chloramphenicol (CAP) is widely used in the food industry and animal husbandry due to its effective antibiotic effect active against gram-positive and gram-negative microorganisms. However, research shows that it can cause serious adverse reactions and side effects in the human body. In order to effectively monitor the residues of CAP, a novel and simple photoelectrochemical (PEC) sensor for sensitive detection of CAP is fabricated based on M-TiO 2 -CdTe QDs/CdS QDs composite. The results show that the prepared M-TiO 2 not only retains the original morphology and structure of MIL-125(Ti), but also exhibits more abundant pore structure and good photoelectrochemical properties. Compared with M-TiO 2 , the as-prepared M-TiO 2 -CdTe QDs/CdS QDs composite exhibits excellent PEC performances including about ninefold enhancement of photocurrent intensity, which is ascribed to the large surface of M-TiO 2 and the introduction of CdTe QDs and CdS QDs. Based on the selective inhibitory effect of CAP in the photocurrent intensity of the M-TiO 2 -CdTe QDs/CdS QDs PEC system, a novel PEC sensor for CAP concentration determination is constructed. The designed PEC sensor demonstrates a linear range from 1 to 140 nmol L −1 with a detection limit of 0.14 nmol L −1 (S/N = 3). Moreover, the method is applied to real milk samples to quantify the CAP residues with spiked recoveries in the range of 96.3–106%, and the possible detection mechanism of the M-TiO 2 -CdTe QDs/CdS QDs PEC system is also discussed. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PARASITOLOGY RESEARCH
·
2022
·Vol. 121
·期 2
·675-689
·
IF 2.383
Haseeb Muhammad,Huang Jianmei,Lakho Shakeel Ahmed,Yang Zhang,Hasan Muhammad Waqqas,Ehsan Muhammad,Aleem Muhammad Tahir,Memon Muhammad Ali,Ali Haider,Song Xiaokai,Yan Ruofeng,Xu Lixin,Li Xiangrui
Eimeria maxima (E. maxima) are an intracellular apicomplexan protozoan that causes intestinal coccidiosis in chickens. The purpose of this research was to develop a novel delivery approach for recombinant E. maxima (rEm) 14-3-3 antigen to elicit enhanced immunogenic protection using poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NPs) against E. maxima challenge. The morphologies of prepared antigen-loaded NPs (PLGA/CS-rEm14-3-3 NPs) were visualized by a scanning electron microscope. The rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs–immunized chicken-induced changes of serum cytokines, IgY-antibody level, and T-lymphocyte subsets and protective efficacies against E. maxima challenge were evaluated. The results revealed that encapsulated rEm14-3-3 in PLGA and CS NPs presented spherical morphology with a smooth surface. The chickens immunized with only rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs elicited a significant ( p <0.05) higher level of IFN-γ cytokine, stimulated the proportions of CD4 + /CD3 + , CD8 + /CD3 + T-cells, and provoked sera IgY-antibody immune response compared to control groups (PBS, pET-32a, PLGA, and CS). Whereas, PLGA-rEm14-3-3 NP–immunized chicken provoked a higher level of IFN- γ production and IgY-antibody response rather than CS-rEm14-3-3 and bare antigen, relatively. The animal experiment results ratified that PLGA-rEm14-3-3 NP–immunized chicken significantly alleviated the relative body weight gain (%), decreased lesion score, and enhanced oocyst decrease ratio compared to CS-rEm14-3-3 NPs and only rEm14-3-3. The anti-coccidial index of the chicken vaccinated with the PLGA-rEm14-3-3 NPs was (180.1) higher than that of the Cs-rEm14-3-3 NPs (167.4) and bare antigen (165.9). Collectively, our statistics approved that PLGA NPs might be an efficient antigen carrier system (Em14-3-3) to act as a nanosubunit vaccine that can improve protective efficacies in chicken against E. maxima challenge.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 7
·4510-4522
·
IF 2.779
Jin Dan,Yang Xiaolong,Wei Ying
Recently, cobalt-nickel nanoparticles with ferromagnetic properties are often used in wave absorption materials and sensors. In this paper, after surface treatment of carbon fiber, a simple solvothermal method was used to grow cobalt-nickel alloy particles on the surface of carbon fiber, which was expressed as CoNi alloy/carbon fibers composite. The surface treatment process of carbon fiber, the structures, morphology, chemical, and electromagnetic parameters of CoNi alloy/CFs composites were analyzed in detail. In the composite material, more loss mechanisms are introduced to balance impedance matching, thus achieving designable electromagnetic parameters, ultra-thin thickness, low density, wide absorption frequency band, and strong electromagnetic absorption capacity. The effects of permittivity and permeability on the absorbing properties of the composites were compared under different CoNi alloy content (15–45%). The absorption frequency of composites moves to high-frequency and shows better performance, with the minimum RL of − 55.4 dB at 17.1 GHz and the effective absorption bandwidth is 4.1 GHz (thickness 1.2 mm). Accordingly, as-prepared CoNi alloy/CFs composites could be used as electromagnetic wave absorbing materials with great application prospects.
✓ 已验证
产品关联
DOI
高影响因子
IONICS
·
2022
·Vol. 28
·期 5
·2349-2362
·
IF 2.961
Han Jia-Jun,Yan Qi-ruo,Chen Zhao-wen,Wang Zhen,Chen Chao
Polyaniline (PANI) has been widely used for the energy storage applications either as a conducting agent or directly as an electroactive material due to the tunable pseudocapacitive performance owing to its various oxidation states. Nanocomposites including PANI, PANI/graphene oxide (PANI/GO), and PANI/GO/Cr-MOF were synthesized via a novel in situ chemical oxidative polymerization method including two oxidants. The structure and morphology of composites were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM) measurements. Furthermore, PANI, PANI/GO, and PANI/GO/Cr-MOF were assembled as positive electrode materials into a button-type supercapacitor for electrochemical performance testing; the electrochemical performances of the composites were characterized by cyclic voltammetry (CV), galvanostatic charge–discharge (GCD), and electrochemical impedance spectroscopy (EIS) techniques in detail. The ASC (asymmetric supercapacitors) possessed an extended potential window (0.8 V), an extraordinary specific capacitance (243.125 F/g at 0.5 A/g, 243 F/g at 1 A/g, and 242.5 F/g at 2 A/g, and these data fully demonstrate that this material has excellent rate performance), a remarkable cycling property (90.72% capacitance retention after 5000 cycles), and a satisfactory average energy and power density (21.56 wh/kg and 3.6 kW/kg).
✓ 已验证
产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2022
·Vol. 85
·期 4
·365-371
·
IF 2.213
Mei Ling,Tang Hao,Wang Shuai,Guo Yong,Liang Xiaojing
Sub-2-µm sea-urchin-like TiO 2 microspheres were prepared through the one-step solvothermal method. Results of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) show that there are a lot of nanorods coming out from the center of the TiO 2 microsphere, making it look like a sea urchin. The morphology and structure of these microspheres are very suitable for chromatographic packing. During the separation of inorganic anions on this new stationary phase, the retention mechanism is electrostatic interaction, and the pH of the mobile phase plays an important role in retention behaviors. With this new stationary phase for separation of organic anions and nucleobases, Lewis acid–base interaction is more outstanding, which can cause peak broadening and tail for larger conjugated structure compounds. The separation of aromatic hydrocarbons on this new stationary phase demonstrates that Lewis acid–base interaction has a favorable influence on the separation of electron-rich aromatic hydrocarbons under normal phase chromatographic conditions. Graphical abstract
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-11
·
IF 6.408
Jia Shicong,Tang Ruizhi,Zhang Shuai,Gao Zheng,Gong Bolin,Ma Shujuan,Ou Junjie
A kind of core–shell composite microsphere (CM) with nano-on-micro structure was synthesized via grafting amine-modified nanodiamonds onto the surface of monodisperse nonporous polymeric microsphere. In this way, the agglomeration of nanodiamond particles in the solution was avoided. After modification with pyrogallol groups, CM could chelate titanium ions (Ti 4+ ) and thus be utilized as immobilized metal affinity chromatography (IMAC) sorbent to enrich phosphopeptides from biological samples. The resulting Ti 4+ -CM exhibited high enrichment efficiency and specificity to phosphopeptides. A total of 106 of unique phosphopeptides mapped to 29 phosphoproteins were clearly identified from 5 µL of a milk digest after enrichment. Owing to the strong chelation between Ti 4+ and pyrogallol ligands, the Ti 4+ is not released from the sorbent after completion of the enrichment process. As a result, the Ti 4+ -CM sorbent could be reused, and no significant loss of enrichment efficiency occurred even on the fourth run employing a β-casein digest as the sample. The strategy adopted presents a new way to prepare a high-performance reusable IMAC sorbent. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science China-Materials
·
2022
·Vol. 65
·期 6
·1453-1462
·
IF 8.64
Sun Qiuhong,Zhu Kai,Ji Xiangli,Chen Dandan,Han Cheng,Li Ting-Ting,Hu Yue,Huang Shaoming,Qian Jinjie
The design and preparation of non-noble metal catalysts with high catalytic activity and robust stability are important in the research of metal-air batteries and fuel cells. Here, a three-dimensional (3D) hierarchically ordered porous carbon nanomaterial was conveniently synthesized with zeolite-imidazole framework (ZIF-8) carbonization using the silica-template method and carbon nanotube (CNT) growth. The addition of an iron source endows the porous mFeNC-CNT with Fe-based nanoparticles and abundant atomically dispersive Fe-N x sites from its nitrogen-incorporated graphitic carbon matrix. As a result, the 3D porous structure reduces the charge transport resistance, and the iron and nitrogen codoped carbon exhibits excellent catalytic activity for oxygen reduction reaction (ORR) similar to that of commercial Pt/C. Meanwhile, the interwoven CNTs obtained under urea catalysis further shorten the ion and electron diffusion pathway. Experimental and theoretical analyses revealed that the optimized mFeNC-CNT has a high ORR activity with a half-wave potential of 0.908 V and a large open-circuit voltage (1.556 V) when applied on zinc-air batteries. This work provides a promising strategy for the rational design and facile synthesis of high-performing non-noble metal-based electrocatalysts for energy storage, conversion, and transport applications.
✓ 已验证
产品关联
DOI
高影响因子
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
·
2022
·Vol. 47
·期 6
·7123-7134
·
IF 2.807
Wang Pengjie,Xiong Liping,He Zhongyi,Xu Xin,Hu Jianqiang,Chen Qi,Zhang Renhui,Pu Jibin,Guo Lei
The corrosion inhibition effect and synergistic mechanism of novel imidazoline derivative (SMIF) and benzimidazole (BMZ) in 3.5 wt% simulated concrete solution were studied experimentally and theoretically. The complex inhibitors have the most outstanding inhibition efficiency when SMIF concentration is 0.1 g/L and BMZ is 0.9 g/L, the corrosion inhibition efficiencies of weight loss, Electrochemical Impedance Spectroscopy and Potentiodynamic polarization curve (Tafel) were 88.81%, 86.27% and 90.22%, respectively. Electrochemistry analysis results proved that the corrosion inhibition mechanism of the inhibitors in the corrosion solution was mainly the formation of a protective film on the surface of carbon steel. FT-IR, Energy Dispersive Spectroscopy and X-ray Photoelectron Spectroscopy all proved the existence of the protective film. Molecular dynamics proved that the composite inhibitor molecules could be stable on the surface of carbon steel and when consist of complex inhibitor was 0.1 g/L SMIF and 0.9 g/L BMZ the stable adsorption morphology were the highest. This work confirmed that SMIF and BMZ inhibitors have a good synergistic effect, greatly improving the inhibition efficiency and reducing the cost of corrosion inhibition, and has a certain guiding significance for synergistic inhibition.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）