SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Junjun Shao,Shuai Sun,Da Zhan,Lipeng Pan,Guangzong Min,Xiaobao Li,Kailun Huang,Wei Chen,Likun Yang,Xiang-Yang Liu,Naibo Lin
As a kind of reactive oxygen species, peroxynitrite is related to various diseases closely such as cancer and neurodegenerative diseases. Constructing probes with highly specific ability and a wide linear detection range for peroxynitrite detection is crucial for understanding the pathogenesis of related diseases and optimizing treatments. In this work, we developed a novel luminescent ratiometric fluorescence nanoprobe (PC-CDs) based on carbon dots and phycocyanin. PC-CDs are constructed by amidation reaction between carbon dots and phycocyanin. The nanoprobe we obtained has a good ability of distinguishing peroxynitrite from other reactive oxygen species and interfering substances. Moreover, the linear range of the nanoprobe is 0.5–100 μM and the limit of detection is 0.5 μM when detecting peroxynitrite. In the spiked recovery experiments under phosphate buffered saline (PBS) environment, our nanoprobe has a good recovery performance and the recovery is 99% − 104%, which will be beneficial to the further development of peroxynitrite testing and the research progress of related diseases. Finally, we discuss the quenching mechanism of peroxynitrite for nanoprobe, and found that there is the combination of dynamic and static quenching in the quenching process.
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高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 6
·868-875
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IF 2.613
Feifei Li,Pengfei Fan,Xinbei Chen,Xi Lin,Can Liu,Congcong Hu,Shengyuan Yang,Fubing Xiao
Sensitive detection of doxorubicin (DOX) is critical for clinical theranostics. A novel ratiometric fluorescence strategy based on the inner filter effect (IFE) has been established for the sensitive detection of DOX by designing a ratiometric fluorescence probe. In the presence of DOX, the fluorescence intensity of copper nanoclusters (CuNCs) at 485 nm decreases, and the fluorescence intensity of carbon dots at 560 nm increases. Therefore, DOX can be quantitatively detected by measuring the ratio of the fluorescence intensities at 560 and 485 nm ( F 560 / F 485 ). The F 560 / F 485 ratio exhibits a linear correlation with the DOX concentration in the range from 1.0 × 10 −8 M to 1.0 × 10 −4 M with the detection limit of 3.7 nM. Furthermore, this method was also successfully applied to the analysis of DOX in human plasma samples, affording an effective platform for drug safety management.
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高影响因子
CELL BIOLOGY INTERNATIONAL
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2022
·Vol. 46
·期 6
·933-946
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IF 4.473
Chunliu Tang,Jiefeng Luo,Xianjia Yan,Qiaojuan Huang,Zhenhua Huang,Qi Luo,Yuan Lan,Dingzhi Chen,Baolin Zhang,Menghua Chen,Deyan Kong
Polydopamine nanoparticles are artificial melanin nanoparticles (MNPs) that show strong antioxidant activity. The effects of MNPs on the neuroprotection of mesenchymal stem cells (MSCs) against hypoxic-ischemic injury and the underlying mechanism have not yet been revealed. In this study, an oxygen–glucose deprivation (OGD)-injured neuron model was used to mimic neuronal hypoxic-ischemic injury in vitro. MSCs pretreated with MNPs and then cocultured with OGD-injured neurons were used to investigate the potential effects of MNPs on the neuroprotection of MSCs and to elucidate the underlying mechanism. After coculturing with MNPs-pretreated MSCs, MSCs, and MNPs in a transwell coculture system, the OGD-injured neurons were rescued by 91.24%, 79.32%, and 59.97%, respectively. Further data demonstrated that MNPs enhanced the neuroprotection against hypoxic-ischemic injury of MSCs by scavenging reactive oxygen species and superoxide and attenuating neuronal apoptosis by deactivating caspase-3, downregulating the expression of proapoptotic Bax proteins, and upregulating the expression of antiapoptotic Bcl-2 proteins. These findings suggest that MNPs enhance the neuroprotective effect of MSCs against hypoxic-ischemic injury by inhibiting apoptosis and upregulating antioxidant defense, which could provide some evidence for the potential application of combined MNPs and MSCs in the therapy for ischemic stroke.
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Journal of Materials Research and Technology-JMR&T
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2022
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IF 6.267
Chao Wang,Dayong He,Hui Zhao,Ce Wang,Kaizhong Wang
Levofloxacin is an emerging contaminant that threatens the aquatic organisms and human health. Bi 2 WO 6 -based heterojunction materials exhibit positive photocatalytic degradation activity under visible light with highly stable hierarchical nanostructures for levofloxacin remediation. However, recycling in practical applications is challenging. Herein, a hybrid nanofibrous film comprising BiOBr nanoplates on a layered Bi 2 WO 6 nanofiber was successfully synthesized using electrospinning, a solvothermal method, and successive ionic layer adsorption and reaction (SILAR). The size and quantity of BiOBr nanoplates grown on the fibers are controlled by adjusting the number of SILAR cycles. The BiOBr nanostructure obtained on the surface of the nanofibers controlled the photocatalytic activity of the sample. The resulting film exhibited superior degradation efficiency toward levofloxacin under visible-light irradiation due to its solid heterostructure, large specific surface area, high porosity, and enhanced visible-light response. The degradation efficiency of levofloxacin reached 95.25% after the system was exposed to visible light for 120 min, and five cyclic experiments demonstrated its excellent stability and reuesability. The Bi 2 WO 6 /BiOBr/polyacrylonitrile nanofibrous film provides a new strategy for developing high-tech equipment and other applications in environmental remediation.
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ANALYTICAL CHEMISTRY
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2022
·
IF 8.008
Xuwen Gao,Huimin Zhao,Dongyang Wang,Yuqi Xu,Bin Zhang,Guizheng Zou
Singlet oxygen (1O2) is an important reactive oxygen species (ROS) that is intensively involved in natural photochemical and photobiological processes. Herein, selectively lighting up 1O2 is achieved in the aggregation-induced emission (AIE) of electrochemiluminescence (ECL) from the Zn2+-mediated AIE assembly of Au nanoclusters (Zn2+-AIE-AuNCs). Zn2+-AIE-AuNCs can exhibit efficient AIE ECL and photoluminescence (PL) along with 1O2 generation in energy and charge transfer routes, respectively. The AIE ECL of the Zn2+-AIE-AuNCs/tripropylamine (TEA) system in carbonate buffer is located around 703 nm with the dimeric aggregate of 1O2 as an emitter because electrochemically oxidizing coexisted Zn2+-AIE-AuNCs and TEA in carbonate buffer would promote the oxygen vacancy (Ov) of Zn2+-AIE-AuNCs, which could selectively enable the generation of emissive singlet oxygen in the energy transfer route by effectively transferring the energy from excited singlet Zn2+-AIE-AuNCs to the triplet ground state of dissolved oxygen (3O2). No emissive 1O2 is detected via electrochemically oxidizing the Zn2+-AIE-AuNCs in the case without either carbonate buffer or TEA, and the Zn2+-AIE-AuNCs/TEA system can only exhibit AIE ECL around 485 nm with Zn2+-AIE-AuNCs as the emitter in carbonate-free buffers. Photoexciting Zn2+-AIE-AuNCs merely brings out band-gap-engineered AIE PL around ∼485 nm with Zn2+-AIE-AuNCs as the emitter, which manifests that the 1O2 generated in the charge transfer route via photoexciting Zn2+-AIE-AuNCs is un-emissive. This work not only proposes an effective strategy for AIE with 1O2 as an emitter but also opens a promising way to selectively light up 1O2.
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SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Shuzhen Dou,Zhongshun Wang,Qiye Chen,Nan Lu
The high sensitivity of surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) analysis depends on the enhancement of the signal intensity and the reduction of the background. Here, high-density Si nanotips were prepared as SALDI-MS substrate by taking template-free reactive ion etching for sensitive detection . The high-density Si nanotips act as tiny antennas to concentrate the laser energy and generate photo-induced electrons on a small area, which promotes desorption and ionization of molecule, enhancing the signal intensity. Additionally, the high stability of Si nanotips under laser irradiation ensures low background interference. The applicability of the substrate was demonstrated by the detection of various small molecules, e.g., fatty acids, amino acids , environmental pollutants and drugs. In addition, this substrate can be successfully applied for detecting trace small molecules in complex sample, such as 10 −9 M verapamil in serum and 10 −12 M crystal violet in lake water. Moreover, the banned fungicides left on the fish skin was also detected. The substrate is compatible with imaging, and the endogenous and exogenous substances in fingerprint were visualized by MS imaging. These results demonstrate the easily prepared high-density Si nanotips SALDI-MS substrate is of high detection sensitivity and great value toward large-scale practical applications.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2022
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IF 6.51
Yu Zou,Quan Zhou,Yinan Zhao,Defu Zhi,Huiying Chen,Rui Wang,Benzhi Ju,Shubiao Zhang
Ionizable lipids are the leading vectors for gene therapy. Understanding the effects of molecular structure on efficient gene delivery is one of the most important challenges for maximizing the utility of such lipid vectors. We synthesized an array of pH-responsive and ionizable lipids to investigate the relationship between lipid structure and activity. The optimized lipid (EDM) has double tertiary amines in the headgroup and an ester linker. EDM exhibited efficient DNA and siRNA delivery to, and gene silencing of, A549 cells. EDM has a p K a value of 6.67, which enabled it to quickly escape from the endosome after entering the cell; the ester linkages rapidly degraded and enabled gene release into the cytoplasm. EDM also delivered IGF-1R siRNA to inhibit tumor growth and induce cancer cell apoptosis by efficient inhibition of IGF-1R expression in mice. Our study on the structure–activity relationships of ionizable lipids will facilitate clinical applications.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Menglan Gao,Zhibin Jiang,Xiaobin Liao,Huan Qi,Lei Zhao,Chao Chen,Danielle Westerman
Aliphatic amines, common constituents that contribute to dissolved organic nitrogen (DON), can quickly react with ozone due to the lone electron pair on the nitrogen atom and this may produce carcinogen N -Nitrosodimethylamine (NDMA). 3-(Dimethylamino)-1-propylamine (DMAPA) was chosen as a representative to elucidate the NDMA formation characteristics, kinetic rates, reaction pathways, and influencing factors during ozonation in this study. The results demonstrated that NDMA generated directly from DMAPA during ozonation. Moreover, the NDMA yields increased with ozone dosages. The NDMA molar yield increased and then decreased when the pH raised from 5 to 9, achieving the maximum value at pH 8. Hydroxyl radical (∙OH) played a promotional role in NDMA formation, and its scavenger dramatically cut down its yields. Low levels of Br − facilitated NDMA formation, while the value significantly reduced when Br − was up to 1 mM. The NDMA amount was slightly raised by NO 2 − , but it was inhibited by NH 4 + and NO 3 − . Moreover, it was also depressed by co-existing components in actual lake water. Based on the result of the Gaussian calculation, the LC-MS/MS and GC–MS analysis, four possible transformation pathways were proposed. The radical recombination was verified to be the primary pathway for ozone promoting NDMA formation from DMAPA.
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Bioactive Materials
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2022
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IF 16.874
Zihe Zhai,Wei Ouyang,Yuejun Yao,Yuqi Zhang,Haolan Zhang,Feng Xu,Changyou Gao
Acute lung injury (ALI) is associated with excessive inflammatory response, leading to acute respiratory distress syndrome (ARDS) without timely treatment. A fewer effective drugs are available currently to treat the ALI/ARDS. Herein, a therapeutic nanoplatform with reactive oxygen species (ROS)-responsiveness was developed for the regulation of inflammation. Dexamethasone acetate (Dex) was encapsulated into poly(thioketal) polymers to form polymeric nanoparticles (NPs) ( [email protected] ). The NPs were composed of poly(1,4-phenyleneacetonedimethylene thioketal) (PPADT) and polythioketal urethane (PTKU), in which the thioketal bonds could be cleaved by the high level of ROS at the ALI site. The [email protected] could accumulate in the pulmonary inflammatory sites and release the encapsulated payloads rapidly, leading to the decreased ROS level, less generation of pro-inflammatory cytokines, and reduced lung injury and mortality of mice. RNA sequencing (RNA-seq) analysis showed that the therapeutic efficacy of the NPs was associated with the modulation of many immune and inflammation-linked pathways. These findings provide a newly developed nanoplatform for the efficient treatment of ALI/ARDS.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 4
·2018
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IF 6.208
Yan Zhang,Pei Ma,Zhiguang Duan,Yannan Liu,Yu Mi,Daidi Fan
Lung adenocarcinoma (LAC) is a common lung cancer with a high malignancy that urgently needs to be treated with effective drugs. Ginsenoside Rh4 exhibits outstanding antitumor activities. However, few studies reported its effects on growth, metastasis and molecular mechanisms in LAC. Here, Rh4 is certified to show a strong anti-LAC efficiency in vitro and in vivo. Results of flow cytometry and Western blot are obtained to exhibited that Rh4 markedly restrained cellular proliferation and colony formation by arresting the cell cycle in the G1 phase. Results from a wound healing assay and transwell assays demonstrated that Rh4 is active in the antimigration and anti-invasion of LAC. The analysis of Western blot, immunofluorescence and RT-qPCR confirmed that Rh4 reverses the epithelial–mesenchymal transition (EMT) through upregulating the gene expression of E-cadherin and downregulating that of snail, N-cadherin and vimentin. In vivo results from immunohistochemistry show consistent trends with cellular studies. Furthermore, Rh4 suppresses the Janus kinases2/signal transducer and activator of the transcription3 (JAK2/STAT3) signaling pathway stimulated by TGF-β1. Silencing the STAT3 signal or co-treating with AG490 both enhanced the EMT attenuation caused by Rh4, which revealed that Rh4 suppressed EMT via inhibiting the JAK2/STAT3 signaling pathway. These findings explore the capacity and mechanism of Rh4 on the antimetastasis of LAC, providing evidence for Rh4 to LAC therapy.Keywords:ginsenoside Rh4;lung adenocarcinoma;metastasis;cell cycle;JAK/STAT signaling
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
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IF 5.518
Moumita Maiti,Manas Sarkar,Soumen Maiti,Donghong Liu
This work explores noble metal coupled carbon-based nanohybrid materials specifically for their antibacterial and photo-disinfection capabilities from a broader perspective of health to clean. Using gold (Au) nanoparticles (NPs) on the surface of two different shaped zinc oxide-graphene (ZnO-rGO) nanohybrids such as flake and spherical, this experiment aims to manage a pathogen-free environment while filtering and recycling carcinogenic dye-containing wastewater. The shape-tailored ZnO-rGO-Au hybrid generate a substantial amount of reactive oxygen species ; ROSs: hydroxyl radical (•OH), superoxide (•O 2 - ), singlet oxygen, and ( 1 O 2 ) and focal electrostatic interactions on (gram-negative Escherichia coli and gram-positive Staphylococcus aureus ) bacterial cell membranes . Redox cycling and strong potential binding tendency rapture the structural organization of cells that is clearly reflected in growth kinetics, (3(4, 5-dimethylthiazol-2-yl)−2), 5-diphenyl tetrazolium bromide (MTT), inner permeable/ protein/ malondialdehyde assay, and microscopical images after the morphology dependent physical as well as the chemical effects of nanocomposites . The minimum bactericidal concentration (MBC) or minimum inhibitory concentration (MIC) studies show the Au/rGO-ZnO system to be the very potent antibacterial agent , and that only 0.18 mg/ml represents significant bactericidal activity while their toxicity towards mammalian cells is very low (>2 mg/ml). Furthermore, Au NPs alter the bandgap energy of Au/rGO-ZnO hybrids, causing the formation of ROSs at different pH levels or dosages of the catalyst, which results in excellent photocatalytic activity (96% degradation of Rhodamine B when using 0.75 mg catalyst). Based on a Pseudo-first-order kinetic model , a likely mechanistic pathway towards exceptional dye decomposition has also been identified.
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SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Dongqing Liu,Xinyu Bai,Jing Sun,Danyang Zhao,Chenghui Hong,Nengqin Jia
Based on hollow In 2 O 3 /In 2 S 3 material, an innovative molecularly imprinted photoelectrochemical (PEC) sensor was developed for sensitive detection of glutathione (GSH). Using nicotinamide as a functional monomer , the GSH template molecule was imprinted on hollow In 2 O 3 /In 2 S 3 material with a large specific surface area, which has better selectivity to GSH. The cavernous structure of the material facilitates the enhanced absorption of visible light due to light scattering and reflection. The heterojunction structure between In 2 O 3 and In 2 S 3 also promotes the rapid separation of electron-hole pairs, which significantly enhance the photocurrent response. The constructed sensor exhibits the detection range from 1 μmol L −1 to100 μmol L −1 with a detection limit of 0.82 μmol L −1 (S/N = 3). Meanwhile, the PEC sensor has excellent stability and selectivity, which provides an innovative idea for the actual detection of GSH.
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