TALANTA
·
2025
·
IF 6.1
Xiaodan Qi,Shengnan Zhang,Zhifei Wang,Han Zhang,Lianghui Ma,Lihua Jin,Yehua Shen
The emerging field of nanozymes has introduced unprecedented opportunities and challenges for activated charcoal materials derived from biomass waste. By incorporating specific nanostructures or elements, it is possible to overcome the limitations of traditional activated charcoal, such as insufficient catalytic active sites and poor electron transfer efficiency, thereby unlocking its full potential for various applications. In this study, we successfully synthesized trace Fe-doped activated charcoal (Fe-AC) with a graphene-like structure from biomass waste. The Fe atoms were uniformly dispersed on the activated charcoal support, which possessed a high surface area. This not only significantly increased the number of catalytic active sites but also enhanced electron transfer efficiency, substrate mobility, and collision probability. Compared to pristine activated charcoal, the synthesized Fe-AC exhibited multiple enzyme-mimetic activities, including oxidase-like, peroxidase-like, and catalase-like activities. By leveraging its peroxidase-like activity in conjunction with nitrite-specific diazotization reactions, we developed a portable, smartphone-assisted, on-site ratiometric colorimetric hydrogel sensor for nitrite detection. Utilizing smartphone-based digital imaging, this sensor enabled the quantitative analysis of nitrite at concentrations ranging from 1 to 200 μmol/L, with a detection limit as low as 1 μmol/L. The approximate range of hazardous nitrite concentrations could be easily identified with the naked eye, and the proposed strategy was successfully applied to real sample analysis. This sensor not only maximizes the utilization of waste resources, thereby reducing production costs, but also offers greater economic feasibility and environmental sustainability. Given these advantages, it holds promise for broader applications in various fields.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2025
·
IF 5.4
Jingyu Li,Bing Fang,Yao Wu,Yuhang Sun,Yue Liu,haina gao,Ming Zhang
Egg yolk phospholipids are primarily composed of various fatty acids, lysophosphatidylcholine and lysophosphatidylethanolamine. Existing studies demonstrated the neuroprotective activity of egg yolk phospholipids, however, which products of these compounds after digestion executive the function have not been clarified. The objective of this study was to investigate the neuroprotective effects of different structural components in egg yolk phospholipids based on a DMNQ-induced oxidative damage in HT22 cells. The findings demonstrate that pre-treatment with diverse egg yolk phospholipid components, particularly the polyunsaturated fatty acids, markedly elevated cell viability, superoxide dismutase activity, diminished ROS generation, reduced malondialdehyde levels and elevated mitochondrial membrane potential. Furthermore, RNA-Seq analysis demonstrated that unsaturated fatty acids exert its neuroprotective effects by upregulating genes involved in cell proliferation (JAG2, YPEL3), nervous system development (NTF5), DNA damage repair (H2AX), and other related processes. These findings provide a theoretical basis for future studies on the characteristic structure of egg yolk phospholipids with profound neuroprotective effects.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Ying Luo,Na Li,Shuo Lv,Deyang Li,Yuanyuan Hu,Xiaoyang Liu,Dayong Zhou
The whole oyster and its three independent parts (adductor muscle, visceral mass and mantle) were processed into ready-to-eat (RTE) samples and stored at 40 °C for 8 weeks. During storage, the whole oyster samples showed liquid exudation and progressive darkening. In contrast, the color of the visceral mass sample, mantle sample and whole oyster sample deteriorated more obviously than that of the adductor muscle sample. This may be due to the higher contents of transition metal ions, lipids and reducing sugars in the formers, which promote lipid oxidation, Maillard/Maillard-like reaction, and phenolic oxidation - the main cause of color deterioration. The exuding liquid, containing browning reaction substrates such as lipids, sugars and polyphenols as well as browning reaction products, directly stains the adductor muscle while also indirectly accelerating its browning reaction, ultimately leading to uniform discoloration throughout the whole oyster sample.
✓ 已验证
产品关联
DOI
高影响因子
BIOLOGY OF REPRODUCTION
·
2025
·
IF 3
Zhang Yingying,Zhang Eryun,Li Ling,Ren Yongwei,Zhang Jinqiu,Zheng Qiutong,Sun Minya,Zhao Shaojie,Cui Yugui,Gu Ying,Xu Zhice
The risk of multiple placental thrombosis is significantly elevated in preeclampsia, and the vascular endothelium plays a vital role in coagulation processes through the secretion of Integrin alpha 2b, von Willebrand factor, and tissue factor. However, the underlying pathological mechanisms remain unclear. In this study, placental blood vessels were collected from both preeclampsia and normal pregnancies. Protein levels of Integrin alpha 2b were found to be up-regulated in preeclampsia samples compared to controls, indicating enhanced coagulation function in preeclampsia placental tissue. Additionally, higher cholesterol levels of plasma and lower AlkB homolog 1 expression in placental blood vessels of preeclampsia were found, overall DNA N6-methyldeoxyadenosine levels were up-regulated. Interestingly, the DNA N6-methyldeoxyadenosine level at the Integrin alpha 2b promoter was decreased. Cholesterol overload experiments in human umbilical vein endothelial cells demonstrated that cholesterol regulates the protein expression of AlkB homolog 1 and Integrin alpha 2b in a concentration-dependent manner. Furthermore, Integrin alpha 2b protein expression was increased following AlkB homolog 1 knockout, but no changes were observed when cholesterol was incubated with AlkB homolog 1 knockout cells. These findings provide critical insights into the roles of cholesterol levels, AlkB homolog 1, and Integrin alpha 2b in coagulation function within placental blood vessels. This information may contribute to further investigations of potential therapeutic targets and early prevention strategies for placental thrombosis in preeclampsia.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Ling Yang,Hengjia Ni,Chenglong Li,Xiya Zhang,Kai Wen,Yuebin Ke,Huijuan Yang,Weimin Shi,Suxia Zhang,Jianzhong Shen,Zhanhui Wang
Antibody-based sensors are the most widely used for screening antibiotic residues in food samples. However, these sensors always suffer from cross-reacting antibiotics which are able to bind the employed antibodies in a manner similar to the target, thus resulting in inaccurate quantification. In this study, we developed a highly specific and sensitive chemiluminescence aptasensor for sulfamethazine (SMZ) by employing the supernormal selectivity of a new selected aptamer. Four SMZ-specific aptamers ( K d ranging from 79 to 274 nM) were selected in vitro by MB-SELEX with an intentional counter selection to avoid the recognition other sulfonamides, which is impossible to achieve in the corresponding procedure of antibody production. The binding mechanism of aptamers for SMZ was further analyzed and clarified with molecular simulation and docking. Under optimum conditions, the chemiluminescence aptasensor based on the highest affinity aptamer named SA07 provided a limit of detection of 0.92 ng/mL with negligible cross-reactivity with the 27 tested sulfonamides. The developed aptasensor was applied to milk samples, and achieved good accuracy and precision. These results demonstrated that the integration of an aptamer and chemiluminescence in a sensor platform is a promising method for the sensitive, specific, rapid and accurate detection of antibiotic residues of interest in food samples.
✓ 已验证
DOI
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2013
·
IF 3.975
Zhaobin Zhang,Libei Sun,Ying Hu,Jian Jiao,Jianying Hu
Parabens are p -hydroxybenzoic acid esters that have been used extensively as preservatives in foods, cosmetics, drugs and toiletries. These intact esters are commonly detected in human breast cancer tissues and other human samples, thus arousing concern about the involvement of parabens in human breast cancer. In this study, an in vitro nuclear receptor coactivator recruiting assay was developed and used to evaluate the binding activities of parabens, salicylates and benzoates via antagonist competitive binding on the human oestrogen-related receptor γ (ERRγ), which is known as both a diagnostic biomarker and a treatment target of breast cancer. The results showed that all of the test parabens (methyl-, ethyl-, propyl-, butyl- and benzylparaben) possessed clear inverse antagonist activities on ERRγ, with a lowest observed effect level (LOEL) of 10 − 7 M and the 50% relative effective concentrations (REC50) varying from 3.09 × 10 − 7 to 5.88 × 10 − 7 M, whereas the salicylates possessed much lower activities and the benzoates showed no obvious activity. In silico molecular docking analyses showed that parabens fitted well into the active site of ERRγ, with hydrogen bonds forming between the p -hydroxyl group of parabens and the Glu275/Arg316 of ERRγ. As the paraben levels reported in breast cancer tissues are commonly higher than the LOELs observed in this study, parabens may play some role via ERRγ in the carcinogenesis of human breast cancer. In addition, parabens may have significant effects on breast cancer patients who are taking tamoxifen , as ERRγ is regarded as a treatment target for tamoxifen.
✓ 已验证
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2020
·
IF 3.72
Guanhua Liu,Mei Yang,Jianyu Fu
Terpenes and their derivatives are vital components of tea aroma. Their constitution and quantity are highly important criteria for the sensory evaluation of teas. Biologically, terpenes are involved in chemical resistance of tea plant against biotic and/or abiotic stresses . The goal of this study is to identify volatile terpenes of tea plants implicated in defense against herbivores and to identify terpene synthase ( TPS ) genes for their biosynthesis . Upon herbivory by tea geometrid ( Ectropis obliqua Prout), tea plants were found to emit two sesquiterpenes, ( E , E )-α-farnesene and ( E )-nerolidol, which were undetectable in intact tea plants. The induced emission of ( E , E )-α-farnesene and ( E )-nerolidol suggests that they function in either direct or indirect defense of tea plants against the tea geometrid. Candidate TPS genes were identified from the transcriptomes of tea plants infested by tea geometrids. Two dedicated sesquiterpene synthases, Cs AFR and Cs NES2, were identified. Cs AFR belongs to the TPS-b clade and can catalyze the formation of ( E , E )-α-farnesene from ( E , E )-FPP. Cs NES2 belongs to the TPS-g clade and can synthesize ( E )-nerolidol using ( E , E )-FPP. The two genes were also both dramatically upregulated by herbivore damage. In summary, we showed that two novel sesquiterpene synthase genes CsAFR and CsNSE2 are inducible by herbivory and responsible for the elevated emission of herbivore-induced ( E , E )-α-farnesene and ( E )-nerolidol, which are implicated in tea plant defense against herbivores.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Miao Tang,Xiao Zhang,Dongli Sun,Yao Yao,Yunchun Zhao,Weidong Fei,Caihong Zheng
The great challenge in developing safe medications for placenta-derived diseases is to reduce or eliminate fetal drug exposure while still providing the necessary therapeutic effect. Rapid advances in nanotechnology have brought opportunities for the therapy of placenta-derived disease through accumulating the drug in the placenta while reducing its placental penetration. Among various nanocarriers, liposomes are regarded as an ideal type of carrier for placental drug delivery due to their biosafety and biodegradability. However, their placental retention effect with different particle sizes has not been studied. This research aimed to explore a suitable size of liposomes for placenta drug delivery. Cy 5 dye was chosen as a model molecule for tracing the distribution of three different-sized liposomes (∼80 nm , 200 nm, and 500 nm) in ICR pregnant mice. The stability, cytotoxicity, and cellular uptake study of Cy 5-loaded liposomes were performed. The in vivo fluorescence studies on ICR pregnant mice suggested that the particle size of liposomes was positively correlated with the degree of liposome aggregation in the placenta. The ratio of fluorescence in the placenta and fetus section (P/F value) was proposed to evaluate the placental retention effect of different-sized liposomes. The results showed that the liposomes with 500 nm had the highest P/F value and thus exhibited the strongest placental retention effect and the weakest placental penetration ability. Moreover, liquid chromatography-mass spectrometry analysis confirmed the reliability of the fluorescence section analysis in exploring the placental retention effect of nanovehicles. In general, this study introduced a simple and intuitive method to evaluate the placental retention effect of nanoplatforms and defined a suitable size of liposomes for placenta-derived disease drug delivery.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BIOLOGICAL CHEMISTRY
·
2013
·Vol. 288
·期 50
·35671-35682
·
IF 4.651
Jie Li,Ke Yao,Xiaoning Yu,Xinran Dong,Lishe Gan,Chenqi Luo,Yalin Wu
Lipofuscin accumulation in retinal pigment epithelial (RPE) cells of the eye implicates the etiologies of Stargardt disease and age-related macular degeneration, a leading cause of blindness in the elderly. Here, we have identified a previously unknown RPE lipofuscin component. By one- and two-dimensional NMR techniques and mass spectrometry, we confirmed that this compound is a new type of pyridinium bisretinoid presenting an unusual structure, in which two polyenic side chains are attached to adjacent carbons of a pyridinium ring. This pigment is a light-induced isomer of isoA2E, rather than A2E, referred to as iisoA2E. This pigment is a fluorescent lipofuscin compound with absorbance maxima at ∼430 and 352 nm detected in human, pig, mouse, and bovine eyes. Formation of iisoA2E was found in reaction mixtures of all-trans-retinal and ethanolamine. Excess intracellular accumulation of this adduct in RPE cells in vitro leads to a significant loss of cell viability and caused membrane damage. Phospholipase D-mediated phosphodiester cleavage of the A2PE series generated isoA2E and iisoA2E, in addition to A2E, thus corroborating the presence of isoA2PE and iisoA2PE that may serve as biosynthetic precursors of isoA2E and iisoA2E.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2020
·
IF 8.247
Junying Zhao,Dongjiang Chen,Bismark Boateng,Guangfeng Zeng,Yupei Han,Cheng Zhen,John B. Goodenough,Weidong He
Separator-lithium anode interface featured with uniform lithium-ion plating/stripping is of paramount importance for the safe operation of batteries. Lithium anode paired with traditional polyolefin separator is considerably challenged with uncontrolled formation of Li dendrites, giving rise to undesirable rate capacity and cycle life. In this work, through an electrophoretic deposition technique, hexafluoropropylene separator embedded with atomic interlamellar lithium-channel phyllosilicate clay is realized. With large absorption with electrolyte, the separator with interlamellar spacing of 1.31 nm provides abundant active sites for Li + and enables fast transfer of lithium ions, giving rise to an ionic conductivity of 5.66 × 10 −4 S cm −1 at ambient temperature. The parallel interlayers unify the direction of Li + flows, which leads to the uniform deposition of Li + on the anode and effectively eliminates lithium dendrite issues in Li + plating/stripping processes. The cell assembled with the robust polymer/clay separator is more advantageous than state-of-the-art in the literature, delivering a reversible capacity of 152 mAh g −1 with a 98.5% capacity retention after 300 cycles at 0.5C.
✓ 已验证
DOI
高影响因子
Frontiers of Environmental Science & Engineering
·
2019
·Vol. 14
·期 2
·1-11
·
IF 3.883
Jiang Wenchao,Tang Ping,Liu Zhen,He Huan,Sui Qian,Lyu Shuguang
Hydroxyl radicals (HO•) show low reactivity with perchlorinated hydrocarbons, such as carbon tetrachloride (CT), in conventional Fenton reactions, therefore, the generation of reductive radicals has attracted increasing attention. This study investigated the enhancement of CT degradation by the synergistic effects of hydroxylamine (HA) and formic acid (FA) (initial [CT] = 0.13 mmol/L) in a Fe (II) activated calcium peroxide (CP) Fenton process. CT degradation increased from 56.6% to 99.9% with the addition of 0.78 mmol/L HA to the CP/Fe(II)/FA/CT process in a molar ratio of 12/6/12/1. The results also showed that the presence of HA enhanced the regeneration of Fe(II) from Fe(III), and the production of HO• increased one-fold when employing benzoic acid as the HO• probe. Additionally, FA slightly improves the production of HO•. A study of the mechanism confirmed that the carbon dioxide radical (CO 2 • − ), a strong reductant generated by the reaction between FA and HO•, was the dominant radical responsible for CT degradation. Almost complete CT dechlorination was achieved in the process. The presence of humic acid and chloride ion slightly decreased CT removal, while high doses of bicarbonate and high pH inhibited CT degradation. This study helps us to better understand the synergistic roles of FA and HA for HO• and CO 2 • − generation and the removal of perchlorinated hydrocarbons in modified Fenton systems.
✓ 已验证
DOI
高影响因子
Nano Research
·
2020
·Vol. 13
·期 2
·412-418
·
IF 8.183
Yang Kai,Jia Langlang,Liu Xinhua,Wang Zijian,Wang Yan,Li Yiwei,Chen Haibiao,Wu Billy,Yang Luyi,Pan Feng
Graphite as a positive electrode material of dual ion batteries (DIBs) has attracted tremendous attentions for its advantages including low lost, high working voltage and high energy density. However, very few literatures regarding to the real-time observation of anion intercalation behavior and surface evolution of graphite in DIBs have been reported. Herein, we use in situ atomic force microscope (AFM) to directly observe the intercalation/de-intercalation processes of PF 6 − in graphite in real time. First, by measuring the change in the distance between graphene layers during intercalation, we found that PF 6 − intercalates in one of every three graphite layers and the intercalation speed is measured to be 2 µm·min −1 . Second, graphite will wrinkle and suffer structural damages at high voltages, along with severe electrolyte decomposition on the surface. These findings provide useful information for further optimizing the capacity and the stability of graphite anode in DIBs.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）