JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
·
2021
·Vol. 40
·期 1
·1-18
·
IF 11.161
Ma Sharui,Wang Na,Liu Rui,Zhang Rui,Dang Hui,Wang Yubo,Wang Simeng,Zeng Zekun,Ji Meiju,Hou Peng
Background Mannose, a natural hexose existing in daily food, has been demonstrated to preferentially inhibit the progression of tumors with low expression of phosphate mannose isomerase (PMI). However, its function in thyroid cancer still remains elusive. Methods MTT, colony formation and flow cytometry assays were performed to determine the response of thyroid cancer cells to mannose. Meanwhile, mouse models of subcutaneous xenograft and primary papillary thyroid cancer were established to determine in vivo anti-tumor activity of mannose. The underlying mechanism of mannose selectively killing thyroid cancer cells was clarified by a series of molecular and biochemical experiments. Results Our data demonstrated that mannose selectively suppressed the growth of thyroid cancer cells, and found that enzyme activity of PMI rather than its protein expression was negatively associated with the response of thyroid cancer cells to mannose. Besides, our data showed that zinc ion (Zn 2+ ) chelator TPEN clearly increased the response of mannose-insensitive cells to mannose by inhibiting enzyme activity of PMI, while Zn 2+ supplement could effectively reverse this effect. Further studies found that the expression of zinc transport protein ZIP10, which transport Zn 2+ from extracellular area into cells, was negatively related to the response of thyroid cancer cells to mannose. Knocking down ZIP10 in mannose-insensitive cells significantly inhibited in vitro and in vivo growth of these cells by decreasing intracellular Zn 2+ concentration and enzyme activity of PMI. Moreover, ectopic expression of ZIP10 in mannose-sensitive cells decrease their cellular response to mannose. Mechanistically, mannose exerted its anti-tumor effect by inhibiting cellular glycolysis; however, this effect was highly dependent on expression status of ZIP10. Conclusion The present study demonstrate that mannose selectively kills thyroid cancer cells dependent on enzyme activity of PMI rather than its expression, and provide a mechanistic rationale for exploring clinical use of mannose in thyroid cancer therapy.
✓ 已验证
DOI
PubMed
高影响因子
Nano Research
·
2020
·Vol. 13
·期 4
·1133-1140
·
IF 8.183
Liu Mingyang,Lu Yang,Yu Qilin,Yu Shu-Hong
Owing to the stimulus-responsive and dynamic properties, magnetism-driven assembly of building blocks to form ordered structures is always a marvelous topic. While abundant magnetic assemblies have been developed in ideal physical and chemical conditions, it remains a challenge to realize magnetic assembly in complicated biological systems. Herein, we report a kind of biomacromolecule-modified magnetic nanosheets, which are mainly composed of superparamagnetic graphene oxide (γ-Fe 2 O 3 @GO), the tumor-targeting protein transferrin (TF), and the mitochondrion-targeting peptide (MitP). Such large-size nanosheets (0.5–1 μm), noted as L-Fe 2 O 3 @GO-MitP-TF, can successfully in situ assemble on the surface of tumor cells in a size-dependent and tumor cell-specific way, leading to severe inhibition of nutrient uptake for the tumor cells. More significantly, the nanostructures could efficiently confine the tumor cells, preventing both invasion and metastasis of tumor cells both in vitro and in vivo . Moreover, the 2D assemblies could remarkably disrupt the mitochondria and induce apoptosis, remarkably eradicating tumors under near-infrared (NIR) irradiation. This study sheds light on the development of new nano-systems for efficient cancer therapy and other biomedical applications.
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Zhao Jialin,Zhu Zhong-Zheng,Huang Xing,Hu Xiaojun,Chen Hongxia
A triple recognition voltammetric method for the determination of brain natriuretic peptide (BNP) is described. Gold nanoparticles (AuNPs) and magnetic nanoparticles (MagNPs), sized 26 and 310 nm, respectively, were synthesized and characterized by transmission electron microscopy (TEM), FT-IR, dynamic light scattering (DLS), and Z-potential measurements. Antibody-modified MagNPs and methylene blue–labeled aptamer (Apt-MB)–modified AuNPs were used as an identifier, a signal reporter, and an amplifier, respectively. In the presence of BNP, the magnetic gold nanocomposite is formed through cascade conjugation via specific interaction. It then hybridized with complementary DNA (cDNA) on the interface, thereby amplifying the current signal of Apt-MB and increasing the selectivity of the immunoassay. Results obtained demonstrate the development of a highly selective method with a detection limit of 0.56 pg mL −1 and a linear response over the concentration range 1–10,000 pg mL −1 . The standard deviation of the method is < 6% while the recovery ranged from 92.2 to 104.2%.
Schematic representation of triple recognition electrochemical immunosensor based on two functionalized nanoparticles (antibody-modified magnetic nanoparticle (MNP-Ab) and aptamer-modified gold nanoparticle (AuNPs-Apt)) for determination of brain natriuretic peptide (BNP).
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
·
2025
·Vol. 44
·期 1
·330
·
IF 12.8
Liu Dan,Li Runtian,Li Mingzhu,Liang Ying,Wang Zhao,Sun Yang,Ge Pengling
Hepatocellular carcinoma (HCC), often arising from liver fibrosis in nonalcoholic fatty liver disease (NAFLD), remains a leading cause of cancer-related death. Targeting the gut–liver axis offers new therapeutic opportunities to prevent this progression. In this study, colon-targeted chitosan/pectin-based nanoparticles loaded with Astragalus polysaccharide (APs-CS/PT-NPs) were developed to modulate gut microbiota and inhibit liver tumorigenesis. The nanoparticles exhibited robust physicochemical stability and pH-responsive release. In vivo, oral administration of APs-CS/PT-NPs attenuated hepatic steatosis, reduced inflammatory cytokines, and suppressed NAFLD-induced HCC development. 16 S rRNA sequencing revealed restoration of microbial diversity and enhanced production of short-chain fatty acids, especially acetate. Mechanistically, transcriptomic profiling and functional analysis identified acetate as a key mediator, acting via G-protein-coupled receptor 43 (GPR43) to inhibit the NF-κB pathway. These results highlight the therapeutic potential of APs-CS/PT-NPs in modulating the gut–liver axis, rebalancing intestinal microbiota, and suppressing pro-inflammatory signaling. This nanoparticle-based strategy offers a promising food-derived preventive intervention for liver fibrosis–HCC transition. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELL BIOLOGY AND TOXICOLOGY
·
2022
·
IF 6.819
Gao Shuya,Yang Qingchen,Peng Yue,Kong Weixian,Liu Zekun,Li Zhe,Chen Jiawen,Bao Mengmeng,Li Xie,Zhang Yubin,Bian Xiaohong,Jin Liang,Zhang Hanwen,Zhang Yuexin,Sanchis Daniel,Yan Fangrong,Ye Junmei
The sirtuin 6 (SIRT6) participates in regulating glucose and lipid homeostasis. However, the function of SIRT6 in the process of cardiac pathogenesis caused by obesity-associated lipotoxicity remains to be unveiled. This study was designed to elucidate the role of SIRT6 in the pathogenesis of cardiac injury due to nutrition overload-induced obesity and explore the downstream signaling pathways affecting oxidative stress in the heart. In this study, we used Sirt6 cardiac-specific knockout murine models treated with a high-fat diet (HFD) feeding to explore the function and mechanism of SIRT6 in the heart tissue during HFD-induced obesity. We also took advantage of neonatal cardiomyocytes to study the role and downstream molecules of SIRT6 during HFD-induced injury in vitro, in which intracellular oxidative stress and mitochondrial content were assessed. We observed that during HFD-induced obesity, Sirt6 loss-of-function aggravated cardiac injury including left ventricular hypertrophy and lipid accumulation. Our results evidenced that upon increased fatty acid uptake, SIRT6 positively regulated the expression of endonuclease G (ENDOG), which is a mitochondrial-resident molecule that plays an important role in mitochondrial biogenesis and redox homeostasis. Our results also showed that SIRT6 positively regulated superoxide dismutase 2 (SOD2) expression post-transcriptionally via ENDOG. Our study gives a new sight into SIRT6 beneficial role in mitochondrial biogenesis of cardiomyocytes. Our data also show that SIRT6 is required to reduce intracellular oxidative stress in the heart triggered by high-fat diet-induced obesity, involving the control of ENDOG/SOD2.
✓ 已验证
DOI
PubMed
高影响因子
HYPERTENSION RESEARCH
·
2019
·Vol. 43
·期 3
·168-177
·
IF 3.217
Bi Hai-Lian,Zhang Yun-Long,Yang Jie,Shu Qing,Yang Xiao-Lei,Yan Xiao,Chen Chen,Li Zhi,Li Hui-Hua
Atrial fibrillation (AF) is the most common human arrhythmia in clinical practice and may be promoted by atrial inflammation and fibrosis. Ubiquitination is an important posttranslational modification process that is reversed by deubiquitinating enzymes (DUBs). DUBs play critical roles in modulating the degradation, activity, trafficking, and recycling of substrates. However, less research has focused on the role of DUBs in AF. Here, we investigated the effect of ubiquitin C-terminal hydrolase 1 (UCHL1), an important DUB, on the development of AF induced by angiotensin II (Ang II). Male wild-type mice were treated with the UCHL1 inhibitor LDN57444 (LDN) at a dose of 40 μg/kg and infused with Ang II (2000 ng/kg/min) for 3 weeks. Our results showed that Ang II-infused wild-type (WT) mice had higher systolic blood pressure and an increased incidence and duration of AF. Conversely, this effect was attenuated in LDN-treated mice. Moreover, the administration of LDN significantly reduced Ang II-induced left atrial dilation, fibrosis, inflammatory cell infiltration, and reactive oxygen species (ROS) production. Mechanistically, LDN treatment inhibited the activation of multiple signaling pathways (the AKT, ERK1/2, HIF-1α, and TGF-β/smad2/3 pathways) and the expression of CX43 protein in atrial tissues compared with that in vehicle-treated control mice. Overall, our study identified UCHL1 as a novel regulator that contributes to Ang II-induced AF and suggests that the administration of LDN may represent a potential therapeutic approach for treating hypertensive AF.
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 17
·期 1
·236
·
IF 15.7
Jia Yuewen,Du Jigang,Lu Yanqiu,Fang Chenyi,Liu Pingwei,Zhang Sui
Voltage-controlled nanofiltration offers a promising approach for precise, on-demand molecular separation. However, its application in organic solvent nanofiltration (OSN) remains underexplored, given the poor solute dissociation in organic solvents. Here, we fabricate conductive and robust conjugated microporous polymer (CMP) membranes to investigate voltage-controlled OSN. Our results show that while solvent permeance slightly decreases with the applied voltage, due to the uneven electron density distribution of solvent molecules, solute rejection is significantly enhanced. This enhancement arises from a dual-barrier mechanism comprising ion migration in the bulk solution and a Donnan-like equilibrium at the feed-membrane interface under an applied voltage. Specifically, in methanol, applying a positive voltage increases the rejection of positively charged methylene blue (MB) from 8.3 % at 0 V to 91.3 % at 5 V. Our study also reveals that the degree of solute dissociation contributes significantly to the effectiveness of voltage-controlled rejection. Lastly, the successful separation of mixed dyes of similar size, together with the enhanced rejection of real pharmaceutical and agrochemical compounds under applied voltage, highlights the potential of in-situ voltage application in OSN for addressing challenging separations.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
·
2022
·Vol. 32
·期 5
·812-818
·
IF 2.464
Zhou Meichen,Yin Shining,Yu Mingming,Wu Aiying,Wang Cong,Lu Jingguang
“Fufang huangliansu” prescription, as a Chinese patent herbal medicine, is widely applied in clinical practice, and its main physiologically active components are berberine hydrochloride and total glucosides of paeony. In this study, a rapid, simple, and accurate quantitative 1 H NMR method was developed and validated to determine the two main components of “fufang huangliansu” prescription, berberine hydrochloride and total glucosides of paeony. Dimethyl terephthalate was selected as an internal standard, and the signals for berberine hydrochloride at δ 8.94 ppm (H-13) and total glucosides of paeony at δ 7.55 ppm (H-3″, 5″) were selected for the construction of calibration curves. Method validation was performed in terms of specificity, precision, accuracy, repeatability, stability, and linearity. The results indicated that the assay was precise and in line with the China Food and Drug Administration guidance. The assay has been successfully applied to determine the content of berberine hydrochloride and total glucosides of paeony in “fufang huangliansu” obtained from two pharmaceutical companies. This finding indicates that this 1 H NMR method has the potential as a reliable method for quality control of Chinese patent medicines containing berberine. Graphical abstract
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 10
·1-10
·
IF 5.833
Chen Rongyuan,Kan Lun,Duan Fenghe,He Linghao,Wang Minghua,Cui Jing,Zhang Zhihong,Zhang Zhonghou
A novel label-free surface plasmon resonance (SPR) aptasensor has been constructed for the detection of N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots (Nb 2 C-SH QDs) as the bioplatform for anchoring N-gene-targeted aptamer. In the presence of SARS-CoV-2 N-gene, the immobilized aptamer strands changed their conformation to specifically bind with N-gene. It thus increased the contact area or enlarged the distance between aptamer and the SPR chip, resulting in a change of the SPR signal irradiated by the laser (He-Ne) with the wavelength ( λ ) of 633 nm. Nb 2 C QDs were derived from Nb 2 C MXene nanosheets via a solvothermal method, followed by functionalization with octadecanethiol through a self-assembling method. Subsequently, the gold chip for SPR measurements was modified with Nb 2 C-SH QDs via covalent binding of the Au-S bond also by self-assembling interaction. Nb 2 C-SH QDs not only resulted in high bioaffinity toward aptamer but also enhanced the SPR response. Thus, the Nb 2 C-SH QD-based SPR aptasensor had low limit of detection (LOD) of 4.9 pg mL −1 toward N-gene within the concentration range 0.05 to 100 ng mL −1 . The sensor also showed excellent selectivity in the presence of various respiratory viruses and proteins in human serum and high stability. Moreover, the Nb 2 C-SH QD-based SPR aptasensor displayed a vast practical application for the qualitative analysis of N-gene from different samples, including seawater, seafood, and human serum. Thus, this work can provide a deep insight into the construction of the aptasensor for detecting SARS-CoV-2 in complex environments. Graphical abstract
A novel label-free surface plasmon resonance aptasensor has been constructed to detect sensitively and selectively the N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots as the scaffold to anchor the N-gene-targeted aptamer.
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2018
·Vol. 9
·期 1
·1-12
·
IF 12.353
Tian Li,Su Mengke,Yu Fanfan,Xu Yue,Li Xiaoyun,Li Lei,Liu Honglin,Tan Weihong
Liquid interfacial plasmonic platform is emerging for new sensors, catalysis, and tunable optical devices, but also promises an alternative for practical applications of surface-enhanced Raman spectroscopy (SERS). Here we show that vigorous mixing of chloroform with citrate-capped gold nanorod sols triggers the rapid self-assembly of three-dimensional plasmonic arrays at the chloroform/water ( O / W ) interface and produces a self-healing metal liquid-like brilliant golden droplet. The O phase itself generates stable SERS fingerprints and is a good homogeneous internal standard for quantitative analysis. This platform presents reversible O / W encasing in a common cuvette determined just by surface wettability of the container. Both O -in- W and W -in- O platforms exhibit excellent SERS sensitivity and reproducibility for different analytes by the use of a portable Raman device. It paves the way toward a practical and quantitative liquid-state SERS analyzer, likened to a simple UV–Vis spectrometer, that is far superior to typical solid substrate-based or nanoparticle sol-based analysis.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 9
·1661-1668
·
IF 3.741
Wang Lin,Mei Ling,Liu Xiang,Shi Jiankai,Li Yahong,Gu Ning,Cui Rongjing
A nanocomposite consisting of helical carbon nanotubes, polyallylamine hydrochloride and CdSe quantum dots was synthesized and used for the electrochemiluminescent determination of dopamine in presence of peroxodisulfate. The nanocomposite was characterized by transmission electron microscopy, UV-absorption and photoluminescence spectroscopy. The electrochemical process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimized conditions, the ECL intensity decreases linearly with the concentration of dopamine in the 1.0 × 10 -9 mol · L -1 to 2.0 × 10 -5 mol · L -1 range, with a 0.2 × 10 -9 mol · L -1 detection limit. The method represents a rapid, simple and sensitive protocol for the determination of dopamine.
A glassy carbon electrode was modified with a nanocomposite prepared from helical carbon nanotubes, polyallylamine hydrochloride and CdSe quantum dots, and used for electrochemiluminescent determination of of dopamine.
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
·
2015
·Vol. 24
·期 3
·1237-1242
·
IF 0.998
Jiang Wei,Chen Xiaolong,Wu Juan,Xu Shanshan,Tian Renbing
We have developed Fe 3 O 4 @ZnS-based fluorescent magnetic composites as targeted drug delivery carriers via a facile route. The results indicated that the composites exhibited both magnetic and fluorescent properties. Fe 3 O 4 @ZnS possessed high saturation magnetization (68.25 emu/g) at room temperature. Ultraviolet light can be easily obtained by exposing the microspheres to different excitation wavelengths. The drug loading studies showed that Fe 3 O 4 @ZnS-based fluorescent magnetic composites had an excellent drug loading performance. These traits made the composites better for the application of medical imaging and magnetic targeted drug delivery.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）