3 Biotech
·
2019
·Vol. 9
·期 7
·1-9
·
IF 1.786
Yan Hong De,Guo Bing Han,Wang Zhao,Qian Jun Qing
The lipase from Aspergillus oryzae was modified with a surfactant and then observed to exhibit high catalytic efficiency and enantioselectivity for the kinetic resolution of ( RS )-1-phenylethanol. The influential factors of the modified-lipase preparation were investigated, including the surfactant source, the organic cosolvent, and the buffer pH. The optimum modification conditions were found with a surfactant of polyoxyethylene sorbitan monopalmitate, an organic cosolvent of tetrahydrofuran and a phosphate buffer of pH 7.0. In the transesterification of ( RS )-1-phenylethanol with vinyl acetate, the surfactant-modified lipase showed excellent enantioselectivity for the R -isomer ( E > 200), giving an enantiomeric excess of higher than 99% for ( R )-1-phenylethyl acetate at 46.8% conversion with the reaction time of 2 h at 30 °C. The enzymatic activity had barely altered after 30 days even at 50 °C when it was saved in a powdered state. The results indicated that the modification strategy was useful and highly efficient, and that modified A. oryzae lipase was a promising biocatalyst in the kinetic resolution of ( RS )-1-phenylethanol.
研究领域:
生物化学
·
酶工程
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Zi-Jian Chen,Xi-Xia Liu,Zhi-Li Xiao,Hui-Jun Fu,Yu-Ping Huang,Shu-Yi Huang,Yu-Dong Shen,Fan He,Xing-Xing Yang,Bruce Hammock,Zhen-Lin Xu
1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene , and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry . In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%–123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography . The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.
研究领域:
环境生物学
·
毒理学
·
分析化学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 21
·18307-18319
·
IF 2.22
Liu Xiongzhang,Luo Hui,Li Yingrui,Chen Fu,Wang Xian,Gong Rongzhou
Titanium boride (TiB 2 ) particles and fibers were synthesized by the carbothermal method with different calcination temperatures and time. Subsequently, the effects of calcination temperatures and time on microstructure and the yield of TiB 2 fibers were investigated. Further, the dielectric and microwave absorption (MA) properties of TiB 2 /paraffin composites within 2–18 GHz were discussed. As a result, the yield of TiB 2 fibers reached a maximum at 1400 °C for 180 min. The content of TiB 2 was directly proportional to permittivity. The sample containing 80 wt % TiB 2 particles and fibers indicated that the minimum reflection loss ( RL min ) attained − 46.5 dB at 11.6 GHz when the thickness was 1.45 mm and the effective absorption bandwidth (EAB) was 4.64 GHz ( RL <–10 dB) with the thickness of 1.25 mm. The related MA mechanisms were also discussed. The excellent stability, simple production procedure, thin absorbing layer, and wide EAB make TiB 2 become an ideal candidate for microwave absorbing materials applied in high temperatures.
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产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
·
2025
·Vol. 80
·期 7
·1274-1282
·
IF 1.1
Guan Zhimin,Xie Yaoyao,Li Ke,An Junling,Wang Ping
A ratiometric electrochemical aptasensor for the sensitive detection of mucin 1 ( MUC1 ) was designed based on a quadruped DNA walker driven by catalytic hairpin assembly ( CHA ). With the tetrahedral DNA nanostructure as the framework, the four legs that extend out acted as the walking chains. MUC1 bound specifically to the aptamer, activating the quadruped DNA walker and initiating the CHA reaction. The activated quadruped DNA walker hybridized with hairpin DNA1 labeled with methylene blue ( MB-HP1 ) immobilized on the electrode surface, and the toehold area of the MB-HP1 probe was exposed to bind with hairpin DNA2 labeled with ferrocene ( Fc-HP2 ), causing the quadruped DNA walker to be released. It continued to move on the electrode surface and bound with another MB-HP1. Eventually, a large number of electrochemical signal molecules were brought close to the electrode surface, while MB moved farther away. Under optimal conditions, the peak current ratio ( I Fc / I MB ) was linearly correlated with the logarithm of MUC1 concentration from 1 fg/mL to 1 ng/mL, with a detection limit as low as 0.56 fg/mL. In addition, the sensing platform demonstrated good performance in the analysis of serum samples.
研究领域:
生物分析化学
·
纳米生物技术
·
电化学
·
生物传感器
·
分子诊断学
✓ 已验证
DOI
高影响因子
INORGANIC CHEMISTRY
·
2021
·
IF 5.165
Shizhu Song,Tian Qin,Qi Li,Yanqing Wang,Yanfeng Tang,Lifang Zhang,Xiaojuan Liu
Facile synthesis of cost-effective carbon-supported Co single atoms (Co-SAs) exhibits huge potential applications in energy storage and conversion devices. We here report the implantation of Co-SAs into hollow carbon spheres (Co-SAs-HCS) via a facile wet-chemistry strategy followed by controlled pyrolysis. Electron-rich histidine acted as a Lewis base effectively immobilizing Co2+ (Lewis acid) via the electrostatic effect and hydrogen bonds, thus achieving the scalable synthesis of Co-SAs-HCS. We constructed a series of histidine-Co2+ structure models to elucidate the formation of histidine-Co2+ complexes by analyzing their binding energy. X-ray absorption fine-structure results verify that central Co atoms with four N coordination atoms possess a non-planar Co-N4 structure. Electrochemical results indicate that the as-prepared Co-SAs-HCS catalyst shows a low potential difference (0.809 V) between the oxygen evolution reaction potential at 10 mA cm–2 and the oxygen reduction reaction half-wave potential, outperforming the commercial Pt/C catalysts (0.996 V). Moreover, an assembled Zn-air battery based on Co-SAs-HCS exhibits an unexpected long-term durability. We have demonstrated that non-planar Co-N4-1-O2 sites are the source for highly efficient adsorption and dissociation of O2 molecules and then reduction of the free energy of desorption of the intermediates by density functional theory. Our findings provide a new design insight into the exploration of advanced electrocatalysts, which will be applied in the design of green energy devices in the future.
研究领域:
材料科学
·
电化学
·
能源存储与转换
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DOI
高影响因子
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
·
2023
·Vol. 48
·期 12
·16471-16482
·
IF 2.9
Zainuddin Muhd Izzudin Fikry,Ahmad Abdul Latif,Subramaniam Reasmyraj R.
Carbon dioxide (CO 2 ) separation with membrane technology has become the focus of researchers over the past decades due to its lower energy consumption and cheaper alternative. Mixed matrix membrane (MMM) of poly(4-methyl-1-pentene) (PMP) with nano fumed silica particles was studied to determine the feasibility of using MMM to separate CO 2 from nitrogen (N 2 ) gas. The critical concentration of PMP (7 wt%) was utilized to form the membrane in this study in order to ensure the polymer chain entanglement occurs to form a dense structure. Agglomeration of particle can be observed on the surface with scanning electron microscopy (SEM) imaging with 10 and 15 wt% of nano fumed silica loading in PMP matrix. This observation is further confirmed with energy-dispersive X-ray (EDX) mapping across the cross-sectional area which shows clustered nano fumed silica particle with loading of 10 and 15 wt%. With 7.5 wt% filler loading and at 1 bar, the MMM seems to give the best gas permeance performance with CO 2 permeance of 4.62 GPU (100.54 Barrer) and N 2 permeance of 0.20 GPU (4.31 Barrer) which makes the highest ideal selectivity to be about 23, which was the highest ideal selectivity achieved in this study. Meanwhile, when the pressure was increased from 1 to 7 bar, the CO 2 permeability increased almost linearly followed by a steady decline in CO 2 /N 2 ideal selectivity.
研究领域:
膜技术
·
气体分离
·
高分子科学
·
纳米复合材料
·
环境工程
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DOI
高影响因子
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2018
·Vol. 96
·期 12
·2586-2592
·
IF 1.265
Renjie Deng,Kuiyi You,Fangfang Zhao,Pingle Liu,He'an Luo
In this work, a simple method for the highly selective preparation of valuable dinitronaphthalene from 1-nitronaphthalene employing NO 2 as a nitrating agent has been developed. The results demonstrated that HY zeolite as an eco-friendly and stable catalyst exhibits good catalytic performances. The total selectivity to valuable dinitronaphthalene compounds including 1,5-, 1,4-, and 1,3-dinitronaphthalene can reach 87.6 % in our present nitration reaction. Meanwhile, the physico-chemical properties of catalysts were characterized by XRD, FTIR, TG/DTG, BET, and NH 3 -TPD, and the probable nitration reaction mechanism was also suggested in this paper. The present nitration process seems to be a mild, eco-friendly, and economical route for the preparation of valuable dinitronaphthalene compounds.
研究领域:
化学工程
·
催化
·
环境化学
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DOI
高影响因子
RUSSIAN JOURNAL OF COORDINATION CHEMISTRY
·
2014
·Vol. 40
·期 10
·764-767
·
IF 0.629
Li G. B.,Wang S. X.,Su W. Y.,Pan R. K.,Liu K. D.,Liu S. G.
A new cationic complex, [Co(Bpbp) 2 ](ClO 4 ) 2 · DMF · H 2 O, was synthesized by treatment Co(ClO 4 ) 2 · 6H 2 O with 2,6- bis (1-phenylbenzimidazol-2-yl)pyridine (Bpbp) in DMF solvent at room temperature. Its structure was characterized by element analysis, IR, and X-ray single crystal structure analyses (CIF file CCDC no. 970498). For complex, crystal system, triclinic, space group, \(P\bar \imath\) , a = 11.585(13), b = 13.939(16), c = 19.236(2) \(\mathop {\AA}\limits^\prime\) , α = 97.938(2)°, β = 102.814(2)°, γ = 90.556(2)°, V = 2861.1(6) Ǻ 3 , Z = 2. The Co 2+ ion octahedral geometry is coordinated by six nitrogen atoms from two ligand (Bpbp). The antiproliferative activities of the complex were screened by MTT assay against HepG2, Huh7, Eca109 and Eca9706 cancer cell. The complex exhibits inhibition on Eca9706 and Eca109 cancer cell but no HepG2 and Huh7 cancer cell.
研究领域:
无机化学
·
药物化学
·
肿瘤学
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DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2025
·
IF 4.7
Tao Wang,Chao Xiao,Qiao Yu,Zhilin Chen,Shuming Peng
Development of a stable catalyst resistant to steam-induced sintering (10 vol%−15 vol% H 2 O, 450–550 °C) is challenging for catalytic combustion/oxidation of methane. We use the silicalite-1's internal defects to trap PdO clusters to construct a stable catalyst (PdO/silicalite-1). Silicalite-1 with sufficient amounts of silanol defects and ion exchange at alkaline condition are critical for this defect-trapping strategy. PdO/silicalite-1 shows long-term super-stability with respect to the temperatures and steam concentrations. The conversion remained stable over 200 h at 500 °C (10 vol% H 2 O) and declined slightly during 380 h at 550 °C (10 vol% H 2 O). PdO/silicalite-1's catalytic activity deteriorated slightly after hydrothermal aging at 750 °C or thermal aging at 850 °C. Electron microscopy reveals two growth modes for PdO clusters within silicalite-1 (PdO in ) and on the external surface (PdO surf ). PdO surf clusters agglomerate into PdO particles of 5–15 nm directly, but PdO in clusters first fuse into strip-shaped PdO in clusters, then migrate outwards and further agglomerate into PdO nanoparticles .
研究领域:
催化化学
·
材料科学
·
环境化学
·
纳米材料
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产品关联
DOI
高影响因子
FUEL
·
2025
·
IF 7.5
Yanxin Zhang,Xuesi Hong,Yuzhu Shi,Duo Xu,Yueqin Song,Xiaolong Zhou,Jin An Wang
The present study investigated a novel bimetallic Pt-Mo catalyst encapsulated within Silicalite-1 zeolite for propane dehydrogenation (PDH). The catalyst was synthesized via in situ hydrothermal crystallization, followed by direct high-temperature H 2 reduction without conventional calcination, leading to uniform encapsulation of Pt nanoparticles within the zeolite framework and simultaneous removal of the template and organic ligands. PDH performance was evaluated in a continuous-flow fixed-bed microreactor. Comprehensive characterizations (XPS, CO-DRIFTS, NH 3 -TPD, Raman, etc.) reveal that Mo incorporation in 0.4Pt@S-1 catalyst significantly enhances propane conversion while maintaining high propylene selectivity. Moreover, the bimetallic 0.4PtXMo@S-1 catalyst with 0.1 % Mo doping achieved an outstanding performance at 550 °C and a WHSV of 5.49 h −1 (propane conversion: 43 %, propylene selectivity: 97 %, propylene formation rate: 16.27 mol C3H6 ·g Pt -1 ·h −1 ). The prepared catalyst possessed favorable stability and regeneration performance, showing a deactivation rate of only 0.001454 h −1 after four reaction-regeneration cycles. This enhancement is attributed to synergistic effects arising from Mo incorporation, which promotes Pt nanoparticle dispersion, modulates Brønsted /Lewis acidity, and facilitates interfacial electron transfer between Pt and Mo species. These findings highlight the role of Pt-Mo interactions within zeolite frameworks, providing new insights for designing efficient and stable PDH catalysts.
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产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 7.3
Bei Zhang,Yi-Yi Yang,Zheng-Jiong Zhao,Run-Duo Liu,Ling-Ling Feng,Mei-Yan Jiang,Yijun Yuan,Shuheng Huang,Zhe Li,Quan Wang,Hai-Bin Luo,Yinuo Wu
Phosphodiesterase 1 (PDE1) is a subfamily of PDE super enzyme families that can hydrolyze cyclic adenosine monophosphate and cyclic guanosine monophosphate simultaneously. Currently, the number of PDE1 inhibitors is relatively few, significantly limiting their application. Herein, a novel series of quinolin-2(1H)-ones were designed rationally, leading to compound 10c with an IC50 of 15 nM against PDE1C, high selectivity across other PDEs, and remarkable safety properties. Furthermore, we used the lead compound 10c as a chemical tool to explore whether PDE1 could work as a novel potential target for the treatment of inflammatory bowel disease (IBD), a disease which is a chronic, relapsing disorder of the gastrointestinal tract inflammation lacking effective treatment. Our results showed that administration of 10c exerted significant anti-IBD effects in the dextran sodium sulfate-induced mice model and alleviated the inflammatory response, indicating that PDE1 could work as a potent target for IBD.
研究领域:
药理学
·
药物化学
·
炎症性疾病
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DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2017
·Vol. 33
·期 2
·229-234
·
IF 1.228
Cunling YE,Chang LIU,Shen WANG,Zhike WANG
Based on the tunability of ionic liquids (ILs) according to the specific requirement of an application, 1-dodecylimidazolium chloride with amphiphilic structures was chemically fabricated on the surface of filter papers (DIL-FPs) for the first time. After synthesis, DIL-FPs was characterized by scanning electronic microscopy, energy dispersive X-ray spectroscopy and Fourier-transform infrared spectroscopy. DIL-FPs was used as a novel thin-film microextraction (TFME) phase for the preconcentration of amphiphilic bisphenol A from plant oil samples. The related extraction variables were studied in a spiked sunflower seed oil. Under the optimal conditions, the linear range was 5.0 – 1000 μg L−1 with a correlation coefficient of 0.9976. The limit of detection (S/N = 3) and the enrichment factor of the proposed method were 2.7 μg L−1 and 118, respectively. The intra-day precision and inter-day precision for six repeated determinations were 2.3 and 4.9%, respectively. These plant oil samples used in this work were free of bisphenol A contaminations. The recovery study carried out in different plant oil samples and mean recoveries ranged from 77.16 to 97.10%. The developed DIL-FPs extraction film phase followed by HPLC-UV provides a potential pretreatment strategy for the analysis of weak organic acid compounds in plant oil samples.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）