JOURNAL OF MOLECULAR LIQUIDS
·
2015
·
IF 2.515
Airong Xu,Yajuan Zhang,Zhiyong Li,Jianji Wang
In the present work, seven 1-allyl-3-methylimizodalium carboxylate ionic liquids (ILs), including 1-allyl-3-methylimizodalium formate [Amim][HCOO], acetate [Amim][CH 3 COO], propionate [Amim][CH 3 CH 2 COO], butyrate [Amim][CH 3 (CH 2 ) 2 COO], glycollate [Amim][HOCH 2 COO], lactate [Amim][CH 3 CHOHCOO]) and benzoate [Amim][C 6 H 5 COO], have been synthesized and characterized. Density ( ρ ) and conductivity ( σ ) have been determined experimentally for these ILs at 298.15 K and atmospheric pressure. Then molar volume ( V m ), standard entropy ( S °), lattice energy ( U POT ) and molar conductivity ( Λ ) of the ILs have been derived from the density and conductivity data. Ionicity of the ILs has been estimated using the Walden plot. Meanwhile, how anionic structure of the ILs affects the volumetric and conductivity properties of 1-allyl-3-methylimidazolium carboxylate ILs has been discussed from the analysis of structure–property relationship.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 30
·7063-7075
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IF 3.431
Wang Meng,Jiao Yang,Cheng Chunsheng,Hua Jianhao,Yang Yaling
A novel and sensitive method based on nitrogen-doped carbon quantum dots as a fluorescence probe coupled with magnetic solid-phase extraction (MSPE) purification for analysis of folic acid (FA) in human serum samples has been established for the first time. In the developed system, magnetic nanoparticles coated with hexanoic acid (Fe 3 O 4 @C 6 ) were synthesized by a one-step chemical co-precipitation method with good magnetic properties and dispersibility for sample purification, and it is better to be separated from the sample. High fluorescence nitrogen-doped carbon quantum dots (N-CQDs), simply prepared using a one-step hydrothermal method with nitrilotriacetic acid, could be selectively quenched by FA. Based on this phenomenon, a fluorescence assay was proposed for specific determination of FA. Various operational experiment parameters have been studied and optimized in detail. Under the optimum experimental conditions, the detection limit of the proposed method for FA was evaluated to be 0.5 nM (S/ N = 3), while the relative standard deviation (RSD) was 1.2% ( n = 6). Finally, the proposed method was applied for determination of trace levels of FA from human serum samples and quantitative recoveries were achieved within the range of 95.7–103.5%. All of the results showed that the proposed method had significant application in further research.
Schematic of synthesis of N-CQDs and schematic of suggested mode for analysis of folic acid (FA).
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RSC Advances
·
2016
·Vol. 6
·期 20
·16882-16890
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IF 3.289
Kai Dong,Aiguo Zeng,Maoling Wang,Yalin Dong,Ke Wang,Chenning Guo,Yan Yan,Lu Zhang,Xianpeng Shi,Jianfeng Xing
Prodrugs synthesized by different drugs not only overcome the defects of the original drugs, but also significantly enhance their treatment effects. In this study, a novel prodrug, 3,4,5-tributyryl shikimic acid (TBS), for the treatment of ulcerative colitis (UC) was synthesized by shikimic acid (SA) and butyric acid (BA) through the esterification reaction. Furthermore, the anion exchange resin, Amberlite 717 was employed to load the sodium salt of TBS through a batch process. Then the drug-loaded exchange resin (TBSS-IER) was encapsulated in the coating material, Eudragit S100, to prepare the colon-targeting drug resin microcapsule (TBSS-DRM) through an in-liquid drying method. The morphology and structure of TBSS-IER and TBSS-DRM were characterized by scanning electron microscopy (SEM). The in vitro release study demonstrated the good colon-targeting of TBSS-DRM. In the in vivo study, the TBSS-DRM exhibited a good therapeutic effect on the experimental colitis mouse induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). All results indicated that the prodrug was effective for colitis and the resin microcapsule system had good colon-targeting and could be used for the development of colon-targeting preparations.
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AMINO ACIDS
·
2016
·Vol. 48
·期 7
·1601-1617
·
IF 3.196
Yu Haining,Guo Zhengzhao,Shen Shengrong,Shan Weiguang
As being a necessary amino acid, taurine plays an important role in the regulation of neuroendocrine functions and nutrition. In this study, effects of taurine on mice gut microbes and metabolism were investigated. BALB/C mice were randomly divided into three experimental groups: The first group was administered saline (CK), the second was administered 165 mg/kg natural taurine (NE) and the third one administered 165 mg/kg synthetic taurine (CS). Gut microbiota composition in mice feces was analyzed by metagenomics technology, and the content of short-chain fatty acids (SCFA) in mice feces was detected by gas chromatography (GC), while the concentrations of lipopolysaccharide (LPS) and superoxide dismutase (SOD) were detected by a LPS ELISA kit and a SOD assay kit, respectively. The results showed that the effect of taurine on gut microbiota could reduce the abundance of Proteobacteria , especially Helicobacter . Moreover, we found that the SCFA content was increased in feces of the NE group while LPS content was decreased in serum of the NE group; the SOD activity in serum and livers of the NE and CS groups were not changed significantly compare to that of the CK group. In conclusion, taurine could regulate the gut micro-ecology, which might be of benefit to health by inhibiting the growth of harmful bacteria, accelerating the production of SCFA and reducing LPS concentration.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
·
IF 2.857
Xin Zhang,Yang Yang,Zufang Wu,Peifang Weng
In order to investigate the modulatory effect of purple sweet potato anthocyanins (PSPAs) on human intestinal microbiota, PSPAs were prepared by column chromatography and their influence on intestinal microbiota was analyzed by monitoring the bacterial populations and analyzing short-chain fatty acid (SCFA) concentrations at different time points. The numbers (log10 cell/mL) of Bifidobacterium and Lactobacillus/Enterococcus spp., Bacteroides-Prevotella, Clostridium histolyticum, and total bacteria after 24 h of culture in anaerobic fermentation broth containing PSPAs were 8.44 ± 0.02, 8.30 ± 0.01, 7.80 ± 0.03, 7.60 ± 0.03, and 9.00 ± 0.02, respectively, compared with 8.21 ± 0.03, 8.12 ± 0.02, 7.95 ± 0.02, 7.77 ± 0.02, and 9.01 ± 0.03, respectively, in the controls. The results showed that PSPAs induced the proliferation of Bifidobacterium and Lactobacillus/Enterococcus spp., inhibited the growth of Bacteroides-Prevotella and Clostridium histolyticum, and did not affect the total bacteria number. Total SCFA concentrations in the cultures with PSPAs were significantly higher than in the controls (P < 0.05). Moreover, during the fermentation, the PSPAs were partially fragmented to phenolic acids, which may exert a better effect on intestinal microecology, suggesting that PSPAs may have prebiotic-like activity by generating SCFAs and modulating the intestinal microbiota, contributing to improvements in human health.
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Food & Function
·
2016
·Vol. 7
·期 4
·1959-1967
·
IF 2.686
Li Zhou,Wei Wang,Jun Huang,Yu Ding,Zhouqiang Pan,Ya Zhao,Renkang Zhang,Bing Hu,Xiaoxiong Zeng
The effects of several parameters on the extraction yield of total polyphenols from grape seeds by pressurized liquid extraction were investigated. The highest recovery of total polyphenols occurred at 80 °C within 5 min, and a single extraction allowed a recovery of more than 97% of total polyphenols. Following the purification with macroporous resin, the effects of grape polyphenols (>94.8%) on human intestinal microbiota were monitored over 36 h incubation by fluorescence in situ hybridization, and short-chain fatty acids (SCFAs) were measured by HPLC. The result showed that the grape polyphenols promoted the changes in the relevant microbial populations and shifted the profiles of SCFAs. Fermentation of grape polyphenols resulted in a significant increase in the numbers of Bifidobacterium spp. and Lactobacillus–Enterococcus group and inhibition in the growth of the Clostridium histolyticum group and the Bacteroides–Prevotella group, with no significant effect on the population of total bacteria. The findings suggest that grape polyphenols have potential prebiotic effects on modulating the gut microbiota composition and generating SCFAs that contribute to the improvements of host health.
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Scientific Reports
·
2016
·Vol. 6
·期 1
·1-11
·
IF 5.228
Dan Jiang,Fang Zhen,Chin Siew-xian,Tian Xiao-fei,Su Tong-chao
Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium and achieved the highest production rate and yield of hydrogen. Highest H 2 yield of 2.02 mol H 2 /mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °C, while the composition of medium was (g/L): 15.66 glucose, 6.04 yeast extract, 4 tryptone, 3 K 2 HPO 4 , 3 KH 2 PO 4 , 0.05 L-cysteine, 0.05 MgSO 4 ·7H 2 O, 0.1 MnSO 4 ·H 2 O and 0.3 FeSO 4 ·7H 2 O, which was very different from that for cell growth. Sugarcane bagasse and Jatropha hulls were selected as typical tropical biomass wastes to produce sugars via a two-step acid hydrolysis for hydrogen production. Under the optimized fermentation conditions, H 2 yield (mol H 2 /mol-total reducing sugar) was 2.15 for glucose, 2.06 for bagasse hydrolysate and 1.95 for Jatropha hull hydrolysate in a 3L fermenter for 24 h at 35 °C, with H 2 purity of 49.7–64.34%. The results provide useful information and basic data for practical use of tropical plant wastes to produce hydrogen.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Yang Yufang,Shen Dongjun,Long Yijuan,Xie Zhixiong,Zheng Huzhi
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for the intrinsic peroxidase-like activity of ficin and designed experiments to examine its effectiveness in a variety of scenarios. Ficin can transform peroxidase substrates to colored products in the existence of H 2 O 2 . Our results also indicate that the active sites of peroxidase-like activity of ficin are different from that of protease, which reveals that one enzyme may catalyze more than one kind of substrate to perform different types of reactions. On the basis of these findings, H 2 O 2 releasing from MCF-7 cells was detected successfully. Our findings support a wider application of ficin in biochemistry and open up the possibility of utilizing ficin as enzymatic mimics in biotechnology and environmental monitoring.
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POWDER TECHNOLOGY
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2017
·
IF 2.942
Liang Shen,Jinbo Zhu,Lingyun Liu,Huaifa Wang
In order to improve the flotation recovery of fine kaolinite , mixed dodecylamine chloride/fatty acid was used as collector in kaolinite flotation process. The performance of dodecylamine chloride/fatty acid mixture collector on kaolinite flotation was investigated using flotation test and computational method. Surface tension measurement, contact angle measurement and image analysis were also used to study the flotation behavior of dodecylamine chloride and mixture collectors. The flotation results showed that much higher kaolinite flotation recovery was obtained in the presence of dodecylamine chloride/octanoic acid mixture compared with that in the presence of dodecylamine chloride alone. Surface tension measurements indicated that the mixed dodecylamine chloride/octanoic acid collector was more efficient at decreasing the air–water interfacial tension. Molecular dynamics simulation showed that the octanoic acid molecules were interleaved among dodecylamine chloride ions and co-adsorbed at the kaolinite (001) surface. The relative concentration of water molecules near kaolinite (001) surface further decreased in the presence of dodecylamine chloride/octanoic acid compared with that in the presence of dodecylamine chloride alone, which indicated a much stronger hydrophobicity of the kaolinite (001) surface.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
·
IF 3.154
Hongliang Zeng,Cancan Huang,Shan Lin,Mingjing Zheng,Chuanjie Chen,Baodong Zheng,Yi Zhang
Lotus seed resistant starch, known as resistant starch type 3 (LRS3), was orally administered to mice to investigate its effects on the gut microbiota, short-chain fatty acids (SCFAs) production, and mineral absorption. The results showed that mice fed LRS3 displayed a lower level of gut bacterial diversity than other groups. The numbers of starch-utilizing and butyrate-producing bacteria, such as Lactobacillus and Bifidobacterium and Lachnospiraceae, Ruminococcaceae, and Clostridium, respectively, in mice increased after the administration of medium and high doses of LRS3, while those of Rikenellaceae and Porphyromonadaceae decreased. Furthermore, SCFAs and lactic acid in mice feces were affected by LRS3, and lactate was fermented to butyrate by gut microbiota. LRS3 enhanced the intestinal absorption of calcium, magnesium, and iron, and this was dependent on the type and concentration of SCFAs, especially butyrate. Thus, LRS3 promoted the production of SCFAs and mineral absorption by regulating gut microbiota in mice.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Chen Bao,Hongliang Zeng,Yi Zhang,Longtao Zhang,Xu Lu,Zebin Guo,Song Miao,Baodong Zheng
In order to investigate the effects of different preparation methods on Semen coicis resistant starch, the structural properties and prebiotic effects of autoclaving treatment–purified S. coicis resistant starch (GP-SRS3), ultrasonic-autoclaving treatment–purified S. coicis resistant starch (UP-SRS3), enzyme-autoclaving treatment–purified S. coicis resistant starch (EP-SRS3) and microwave-moisture treatment–purified S. coicis resistant starch (MP-SRS3) were studied compared to high-amylose maize starch (HAMS). Fourier transform infrared spectroscopy and Solid-state 13 C NMR spectroscopy showed all of SRS3 displayed B-type crystallite structure, furthermore, MP-SRS3 and UP-SRS3 showed higher degree of ordered structure and degree of double helical structure compared to other samples. Field emission scanning electron microscopy showed MP-SRS3 displayed deeply layered strips and UP-SRS3 exhibited some cavities. Moreover, S. coicis resistant starch could promote the growth of Bifidobacteria adolescentis better compared to high-amylose maize starch, among these samples. MP-SRS3 exhibited best prebiotic effect, which was possible attributed to its rough surface and double helix structure. B. adolescentis displayed a higher survival rate to the simulated gastrointestinal tract environment in SRS3 medium than that in high-amylose maize starch medium, especially MP-SRS3 and UP-SRS3, which partly contributed to its strips and cavities. Overall, MP-SRS3 could be regarded as a potential prebiotic to promote the growth of B. adolescentis for its structural trait.
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Journal of Environmental Sciences
·
2024
·
IF 5.9
Xiao Liu,Xiaolong Zhang,Chunyu Geng,Qinglan Hao,Jie Chang,Xin Hu,Yongwang Li,Botao Teng
Fischer-Tropsch synthesis (FTS) wastewater retaining low-carbon alcohols and acids are organic pollutants as a limiting factor for FTS industrialization. In this work, the structure-capacity relationships between alcohol-acid adsorption and surface species on graphene were reported, shedding light into their intricate interactions. The graphene oxide (GO) and reduced graphene oxide (rGO) were synthesized via improved Hummers method with flake graphite (G). The physicochemical properties of samples were characterized via SEM, XRD, XPS, FT-IR, and Raman. The alcohol-acid adsorption behaviors and adsorption quantities on G, GO, and rGO were measured via theoretical and experimental method. It was revealed that the presence of COOH, C=O and C O species on graphene occupy the adsorption sites and increase the interactions of water with graphene, which are unfavorable for alcohol-acid adsorption. The equilibrium adsorption quantities of alcohols and acids grow in pace with carbon number. The monolayer adsorption occurs on graphene was verified via model fitting. rGO has the highest FTS modeling wastewater adsorption quantity (110 mg/g) due to the reduction of oxygen species. These novel findings provide a foundation for the alcohol-acid wastewater treatment, as well as the design and development of high-performance carbon-based adsorbent materials.
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