ANALYTICAL CHEMISTRY
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2014
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IF 5.825
Ling-Na Zhang,Hao-Hua Deng,Feng-Lin Lin,Xiong-Wei Xu,Shao-Huang Weng,Ai-Lin Liu,Xin-Hua Lin,Xing-Hua Xia,Wei Chen
A green approach is proposed for in situ growth of porous platinum nanoparticles on graphene oxide (PtNPs/GO). The resulting nanocomposite has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate in the presence of hydrogen peroxide. On the basis of the peroxidase-like activity, we used the PtNPs/GO as a signal transducer to develop a colorimetric assay for the direct detection of cancer cells. By using folic acid as a recognition element, a total of 125 cancer cells (MCF-7) can be distinguished by naked-eye observation. We envision that this nanomaterial could be used as a power tool for a wide range of potential applications in biotechnology and medicine.
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高影响因子
Analytical Methods
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2015
·Vol. 7
·期 22
·9649-9654
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IF 1.821
Shang Zhou,Danqun Huo,Changjun Hou,Mei Yang,Huanbao Fa,Cui Xia,Mei Chen
The monitoring of folate receptor levels in living cancer cells has attracted increasing interest; however, the present fluorescent probes used often suffer from interference from nonspecific adsorption on the surface of non-target cells. In the present study, the probe is readily prepared by using folic acid as a template molecule, a mesoporous silica nanoparticle as an imprinting material, and a CdTe quantum dot as a signal transducer for the recognition of folate receptors. This system is expected to offer specific recognition of folate receptors over other substances based on the high affinity of folic acid and folate receptors. The probe is stable and monodispersed in an aqueous suspension, and has little toxicity to living cells and thus can be utilized for targeted A549 cell imaging. The living cell imaging experiments further demonstrate its value in bioimaging cell-surface folate receptors by virtue of its superior biocompatibility, lower cytotoxicity and membrane permeability.
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Journal of Materials Chemistry B
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2013
·Vol. 2
·期 10
·1379-1389
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IF 0
Gan Tian,Wenyan Yin,Junjiang Jin,Xiao Zhang,Gengmei Xing,Shoujian Li,Zhanjun Gu,Yuliang Zhao
Upconversion nanoparticles (UCNPs) are ideal building blocks for fabricating multifunctional theranostic agents for simultaneous diagnosis and therapy. We develop multifunctional nanoparticles based on NaGdF4:Yb/Er@NaGdF4 core–shell UCNPs, which are not only endowed with upconversion luminescence (UCL), magnetic resonance (MR) and X-ray computed tomography (CT) imaging ability, but also could be applied as nanocarriers for targeted drug delivery. We systematically investigate their upconversion luminescent, magnetic and X-ray attenuation properties. Importantly, for the first time, we report the controlled loading and delivery of a mixture of chemotherapeutic anticancer drugs, camptothecin (CPT) and doxorubicin (DOX), through UCNPs-based nanocarriers considering that the combined use of two or more drugs usually exhibits much better therapeutic efficacy than that of a single drug. By conjugating nanoparticles with folic acid (FA), which targets folate receptors over expressed on various types of cancer cells, we further demonstrate targeted tri-modal UCL/MR/CT cell imaging and drug delivery with UCNPs. Our results suggest the as-prepared nanocomposites are highly versatile and could potentially be used for simultaneous imaging and therapeutic applications.
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JOURNAL OF NANOPARTICLE RESEARCH
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2013
·Vol. 15
·期 12
·1-14
·
IF 2.175
Shu Chang,Li Ruixin,Guo Jin,Ding Li,Zhong Wenying
The objective of this study was to report the drug delivery system that can integrate the functional building blocks for optical pH-sensing, cancer cell imaging and controlled drug release into a single nanoparticle. The CD/SAHA-QDs-CS/FA nanoparticles were prepared by in-situ immobilization of ZnSe/ZnS quantum dots (QDs) in β-cyclodextrin (CD) and chitosan (CS) polymer loaded with suberoylanilide hydroxamic acid (SAHA). Synthetic CD/SAHA-QDs-CS/FA nanoparticles were approximately 100 nm in size and with blue fluorescence. The drug encapsulation efficiency of nanoparticles was 22.36 % and the encapsulated drug was released via a controlled release mechanism after a 9 h plateau was reached. The efficiency of the drug release in tumor microenvironments (pH 5.3 buffer solutions) was higher than that in physiological pH 7.4. In vitro cytotoxicity assay results showed that the blank nanoparticles had no cytotoxicity and therefore can be used as the fluorescence tracer, and the SAHA-encapsulated nanoparticles expressed an anticancer effect. Confocal microscopy and in vivo imaging studies showed that the developed nanoparticles had cytotoxicity in resistant cancer cells and preferentially accumulated in tumors. CD/SAHA-QDs-CS/FA nanoparticles with excellent long-term optical properties have great prospects for the development of targeting tracers and anti-tumor biomedical research.
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高影响因子
ANALYTICAL CHEMISTRY
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2013
·
IF 5.695
Ji-Wei Shen,Cheng-Xiong Yang,Lu-Xi Dong,Hao-Ran Sun,Kai Gao,Xiu-Ping Yan
Rational design and fabrication of multimodal imaging nanoprobes are of great significance for in vivo imaging. Here we report the fabrication of a multishell structured NaYF4:Yb/Tm@NaLuF4@NaYF4@NaGdF4 nanoprobe via a seed-mediated epitaxial growth strategy for upconversion luminescence (UCL), X-ray computed tomography (CT), and magnetic resonance (MR) trimodal imaging. Hexagonal phase NaYF4:Yb/Tm is used as the core to provide UCL, while the shell of NaLuF4 is epitaxially grown on the core not only to provide an optically inert layer for enhancing the UCL but also to serve as a contrast agent for CT. The outermost NaGdF4 shell is fabricated as a thin layer to give the high longitudinal relaxivity (r1) desired for MR imaging. The transition shell layer of NaYF4 not only provides an interface to facilitate the formation of NaGdF4 shell but also inhibits the energy transfer from inner upconversion activator to surface paramagnetic Gd3+ ions. The fabricated multishell structured nanoprobe shows intense near-infrared UCL, high r1 value of 3.76 mM–1 s–1, and in vitro CT contrast effect. The multishell structured nanoprobe offers great potential for in vivo UCL/CT/MR trimodal imaging. Further covalent bonding of folic acid makes the multishell structured nanoprobe promising for in vivo targeted UCL imaging of tumor-bearing mice.
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JOURNAL OF NANOBIOTECHNOLOGY
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2013
·Vol. 11
·期 1
·1-12
·
IF 0
Zhou Zhijun,Zhang Chunlei,Qian Qirong,Ma Jiebing,Huang Peng,zhang Xi,Pan Liyuan,Gao Guo,Fu Hualin,Fu Shen,Song Hua,Zhi Xiao,Ni Jian,Cui Daxiang
Background Gastric cancer is 2th most common cancer in China, and is still the second most common cause of cancer-related death in the world. Successful development of safe and effective nanoprobes for in vivo gastric cancer targeting imaging is a big challenge. This study is aimed to develop folic acid (FA)-conjugated silica coated gold nanoclusters (AuNCs) for targeted dual-modal fluorescent and X-ray computed tomography imaging (CT) of in vivo gastric cancer cells. Method AuNCs were prepared, silica was coated on the surface of AuNCs, then folic acid was covalently anchored on the surface of AuNCs, resultant FA-conjugated AuNCs@SiO 2 nanoprobes were investigated their cytotoxicity by MTT method, and their targeted ability to FR(+) MGC803 cells and FR(−) GES-1 cells. Nude mice model loaded with MGC803 cells were prepared, prepared nanoprobes were injected into nude mice via tail vein, and then were imaged by fluorescent and X-ray computed tomography (CT) imaging. Results FA-conjugated AuNCs@SiO 2 nanoprobes exhibited good biocompatibility, and could target actively the FR(+) MGC-803 cells and in vivo gastric cancer tissues with 5 mm in diameter in nude mice models, exhibited excellent red emitting fluorescence imaging and CT imaging. Conclusion The high-performance FA-conjugated AuNCs@SiO 2 nanoprobes can target in vivo gastric cancer cells, can be used for fluorescent and CT dual-mode imaging, and may own great potential in applications such as targeted dual-mode imaging of in vivo early gastric cancer and other tumors with FR positive expression in near future.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2014
·
IF 3.107
Jian Rong Zhang,Zhong Ling Wang,Fei Qu,Hong Qun Luo,Nian Bing Li
A highly sensitive folic acid (FA) detection method based on the fluorescence quenching of polyethylenimine-capped silver nanoclusters (PEI-AgNCs) was put forward. In the sensing system, FA and PEI-AgNCs were brought into close proximity to each other by electrostatic interaction, and a two-step electron-transfer process, in which the electron was transferred from FA to AgNCs through PEI molecule, led to fluorescence quenching. The fluorescence quenching efficiency of PEI-AgNCs was linearly related to the concentration of FA over the range from 0.1 nM to 2.75 μM. Good linear correlation (R2 = 0.9981) and a detection limit of 0.032 nM were obtained under optimum conditions. Moreover, the proposed method was used for the determination of FA in real samples with satisfactory results, and those coexistent substances could not cause any significant decrease in the fluorescence intensity of AgNCs. Therefore, the proposed research system is of practical significance and application prospects.
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高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 13
·5419-5427
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IF 1.821
Xiaoli Qin,Wenjuan Guo,Huijing Yu,Juan Zhao,Meishan Pei
A simple electrochemical sensor based on a novel composite film consisting of multi-walled carbon nanotubes (MWCNTs), a room temperature ionic liquid (RTIL) of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6), and amino functionalized graphene (GR–CO–NH–CH2–CH2–NH2) was constructed for the detection of kanamycin. Firstly, MWCNTs–BMIMPF6 composites were fabricated on the surface of a glass carbon electrode (GCE). The synergy mechanism between the MWCNTs and RTIL has been discussed. Secondly, GR–CO–NH–CH2–CH2–NH2 was modified on the first film, which could greatly improve the conductivity of the electrode. The structure of the resultant graphene oxide (GO) was confirmed by FTIR and SEM. The properties of the aptasensor were characterized by electrochemical methods. Under the optimum conditions, the electrochemical aptasensor exhibited a wide linear range for kanamycin from 0.001 to 100 μM with a low limit of detection of 0.87 nM (S/N = 3). The as-prepared aptasensor showed high sensitivity, reproducibility and stability. Finally, the proposed electrochemical aptasensor was successfully applied for the detection of kanamycin in a real sample.
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ACS Applied Materials & Interfaces
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2015
·
IF 6.723
Yuwei Liu,Jing Bai,Xiaodan Jia,Xiue Jiang,Zhuo Guo
Recently, the chemo-photothermal synergistic therapy has become a potential method for cancer treatment. Herein, we developed a multifunctional nanomaterial for chemo-photothermal therapeutics based on silica and graphene core/shell structure (SiO2@GN) because of the ability of GN to convert light energy into heat. Serum protein was further modified onto the surface of GN (SiO2@GN-Serum) to improve the solubility and stability of GN-based nanoparticles in physiological conditions. The as-synthesized SiO2@GN-Serum nanoparticles (NPs) have been revealed to have high photothermal conversion efficiency and stability, as well as high storage and release capacity for anticancer drug doxorubicin (SiO2@GN-Serum-Dox). The therapeutic efficacy of SiO2@GN-Serum-Dox has been evaluated in vitro and in vivo for cervical cancer therapy. In vitro cytotoxicity tests demonstrate that SiO2@GN-Serum NPs have excellent biocompatibility. However, SiO2@GN-Serum-Dox NPs show higher cytotoxicity than SiO2@GN-Serum and free Dox under irradiation with NIR laser at 1.0 W/cm2 for 5 min owing to both SiO2@GN-Serum-mediated photothermal ablation and cytotoxicity of light-triggered Dox release. In mouse models, the tumor growth is significantly inhibited by chem-photothermal effect of SiO2@GN-Serum-Dox. Overall, compared with single chemotherapy or photothermal therapy, the combined treatment demonstrates better therapeutic efficacy. Our results suggest a promising GN-based core/shell nanostructure for biomedical applications.
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高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 8
·1667-1676
·
IF 4.726
Jiadi Sun,Ye Zhu,Long Meng,Wei Wei,Yang Li,Xiaoya Liu,Yufeng Zheng
We report a potential example of using surface functionalization to provide magnesium alloys (Mg–Ca) with controlled release and corrosion resistance properties. A key feature of this approach is to treat the Mg–Ca surfaces with nanoparticles via electrodeposition that can stably load and controllably release bioactive agents or drugs. These photo-cross-linkable and nano-scale particles were prepared by the self-assembly of an amphiphilic poly(γ-glutamic acid)-g-7-amino-4-methylcoumarin (γ-PGA-g-AMC) with encapsulation of a vitamin; moreover, the size and morphology of the resulting particles were studied. Fluorescence microscopy analysis indicated that Vm was effectively incorporated into the γ-PGA-g-AMC particles. Scanning electron microscopy (SEM) images showed that the colloidal particles could be uniformly electrodeposited on the Mg–Ca alloys. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analyses confirmed the successful anchoring of the particles. After the surface electrodeposition of self-assembled colloidal particles, the in vitro degradation results show that deposition of the particles was found to reduce the degradation rate of the magnesium alloys; moreover, the vitamin was controllably released for up to 20 days. Furthermore, the Mg–Ca substrate functionalized with colloidal particles containing a vitamin significantly promoted the attachment, proliferation and spread of NIH-3T3 normal cells. The entire strategy may be used in various medical devices to create coatings for improved biomedical performance.
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ACS Applied Materials & Interfaces
·
2014
·
IF 5.9
Yanyu Huang,Yi Luo,Wenjie Zheng,Tianfeng Chen
Radiotherapy displays curative potential for cervical cancer management, but radioresistance occurs during long-term therapy. To overcome this limitation, tumor-targeted nanotechnology has been proposed to enhance the radiosensitivity of solid tumors. Herein, we used biocompatible bovine serum albumin nanoparticles (BSANPs) as carriers of organic selenocompound (PSeD) with folate (FA) as the targeting ligand to fabricate a cancer-targeted nanosystem. The combination of PSeD and BSANPs endowed the nanosystem with higher light absorption and reactive oxygen species (ROS) generation owing to their properties of surface plasmon resonance (SPR) effect, heavy metal effect, high refractive index and nanoparticulate interfacial effect. The combined treatment drastically increased the ROS overproduction, VEGF/VEGFR2 inactivation and inhibition of XRCC-1-mediated repair of DNA damage, thus triggering G2/M phase arrest and apoptosis. Taken together, our findings demonstrate the utility of FA-BSANPs as a promising radiosensitizer to improve cancer radiotherapy.
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BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Weiping Wang,Ya-Chun Lu,Hong Huang,Ai-Jun Wang,Jian-Rong Chen,Jiu-Ju Feng
In this report, N, S-codoped fluorescent carbon nanodots (NSCDs) were prepared by a facile, simple, low-cost, and green thermal treatment of ammonium persulfate , glucose, and ethylenediamine . The as-prepared NSCDs displayed bright blue emission with a relatively high fluorescent quantum yield of 21.6%, good water solubility, uniform morphology, and excellent chemical stability, compared to pure CDs. The fluorescence of NSCDs can be significantly quenched by methotrexate (MTX) via fluorescence resonance energy transfer (FRET) between NSCDs and MTX, which was used for highly selective and sensitive detection of MTX with a wide linear range up to 50.0 μM and a low detection limit of 0.33 nM ( S / N =3). Moreover, this method was explored for practical detection of MTX in human serum with satisfied results.
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