PHYTOMEDICINE
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2026
·
IF 8.3
Yin-Chao Tong,Huan Lin,Yang Yang,Zhi-fu Zhou,Yi-Ning Zhang,Juan Wang,Wu-ren Ma,Yongsheng Wang,Wei-Min Zhang,Su-Zhu Qing
Background Antimicrobial resistance (AMR) infection is attracting increasing attention, especially superbug infections. As a result, finding ways to reduce AMR is essential. For the time being, natural compound therapy for reducing AMR is an ideal choice. Purpose This study aims to investigate the mechanism of magnolol reducing the AMR of E. coli in vitro and vivo . Methods The morphology and function of E. coli under magnolol treatment were assessed using a scanning electron microscope, qPCR, RNA-seq, and other methods. Moreover, the in vivo treatment effects of magnolol combined with antibiotics were evaluated by HE and IHC staining. Results In this study, we found that magnolol reduced the resistance of ESBL E. coli to the fourth-generation cephalosporin in vitro via two main mechanisms. Firstly, magnolol disrupts iron ion metabolism by increasing environmental iron uptake significantly (P ≤ 0.01), leading to a significant increase in intracellular ROS (P ≤ 0.01) and membrane damage. Secondly, magnolol significantly inhibits the relative mRNA expression of blaCTX-M-1 (P ≤ 0.01) and the CTX-M-1 enzyme activity in ESBL E. coli. Furthermore, we find that magnolol can inhibit ESBL E. coli in vivo by significantly reducing the TLR4-NFκB p65 pathway ( P ≤ 0.01). Conclusion In a word, our results indicate that magnolol is a natural antibacterial adjuvant with potent inhibitory activity against bacterial resistance and exerts this activity through multiple pathways, which has particular significance for the further study of the AMR mechanism.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
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IF 12.2
Zhihao Sun,Yuxuan Du,Jinfeng Wang,Ran Yin,Gong Zhang,Jixian Wu,Tianyang Lu,Yusen Chen,Zhan Qu,Hongqiang Ren
The rapid fabrication of artificial intelligence-based hardware worldwide is intensifying the electronic waste (e-waste) crisis, threatening ecosystems and demanding urgent, sustainable solutions for metal recovery. Herein, a glucose-mediated hydrothermal strategy is reported for the efficient reclamation of high-value metals from complex e-waste. Glucose functions as a multifunctional ligand, establishing a specific pathway for metal–organic coordination, enabling selective metal recovery. By precisely regulating reaction kinetics and thermodynamic parameters, the proposed metal–ligand hydrothermal strategy exhibited exceptional recovery of 99.16% for iron (Fe) and 98.67% for silver (Ag), while concurrently converting toxic metals such as lead (Pb) and nickel (Ni) into value-added byproducts without any energy-intensive purification steps. Notably, the process was operated within a closed-loop system. All residual solutions were recycled, eliminating secondary wastewater discharge and enabling the use of the entire components present in the resource. Density functional theory calculations and radial distribution function analysis further revealed the molecular basis of selectivity: Fe(III) preferentially formed bidentate chelates with the aldehyde and hydroxyl groups of glucose, a process complemented by electrostatic interactions that promote rapid nucleation and precipitation. In contrast, other metal ions, Ag(I) and Pb(II), are coordinated primarily via single oxygen sites. Technoeconomic analysis confirmed the economic viability of the process, demonstrating a net profit of $2460 per ton of processed e-waste. This study provides a sustainable and economically compelling waste-to-resource platform guided by molecular-level insights into selective coordination chemistry for advanced e-waste recycling.
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Collagen and Leather
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2026
·Vol. 8
·期 1
·8
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IF 10.1
Pu Haoliang,Xiao Hanzhong,Wang Yujia,Huang Xin,Shi Bi
The next-generation on-skin devices are expected to provide identical textural characteristics to skin for accomplishing comfortable long-term wearability. Cattle hide is mainly consisted of type I collagen, which is therefore an ideal collagenous-matrix platform for developing skin-like on-skin devices. However, the raw cattle hide has a high water content that leads to poor wearability. Herein, we developed the skin-like wearable (SW) platform via a brand-new inter-fiber spacer-aided (IFS-aided) drying of cattle hide, where an amphiphilic nonionic surfactant that was capable of thoroughly infiltrating into the 3D hierarchical network of collagen fibers (CFs) was employed to work as the inter-fiber spacer to avoid the sticking of CFs during drying. Moreover, the inter-fiber spacer was easily eluted after drying for obtaining the collagenous matrix with highly dispersed fiber structure. The as-developed SW-platform exhibited ideal wearability by exhibiting full-protein nature, outstanding softness (6.808 mm), exceptional water-vapor permeability (5950 g·m -2 ·d -1 ) and high thermal stability. The hierarchical fiber structure with sufficient inter-fiber space endowed the SW-platform with reversible cross-scale deformations of CFs at both the nanoscale and microscale, which demonstrated its promising application in developing the skin-like and eco-friendly e-skins with outstanding sensing capability and flexible display function. Graphical
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ANALYTICA CHIMICA ACTA
·
2026
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IF 6.1
Yazhou Qin,Xuqing Wang,Mengze Fu,Jikai Mao,Yingsheng He,Jiye Wang
The incorporation of illegal drugs into e-cigarettes poses significant threats to both human health and societal safety. Therefore, the development of accurate and rapid detection methods for illicit drug additives in e-cigarettes is of significant importance. This study integrates deep learning with surface-enhanced Raman spectroscopy (SERS) to develop a highly sensitive detection method for nicotine and four etomidate isomers. First, Raman and SERS detection were performed on five drug standards. Furthermore, density functional theory calculations were employed to analyze the characteristic peak vibration modes of the various substances. Additionally, a liquid-liquid extraction pretreatment method for commercially available e-cigarettes was developed, facilitating the simultaneous detection of nicotine and etomidate isomers. Trace detection was successfully achieved for all five substances. Subsequently, an interpretable deep network model was constructed for the classification and identification of these substances, attaining an accuracy of 97.8%. To further enhance the model's transparency and interpretability, gradient-weighted class activation mapping (Grad-CAM) was utilized to reveal the critical SERS peaks necessary for molecular discrimination. This research provides robust technical support for the rapid on-site screening of illicit drugs in e-cigarettes.
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CARBOHYDRATE POLYMERS
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2026
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IF 13.2
Chenchen Shen,Weiwei Chen,Lin Guo,Yanrui Zou,Shuan Han,Ning Yu,Lin Gan,Jin Huang
Long-range piezoelectric transduction in flexible hydrogels is limited by the “trade-off” of conductive pathways between accelerating charge dissipation and suppressing dipole formation. Here, we introduce a surface-heterojunction strategy converting cellulose nanocrystals (CNCs) into conductive dipolar nanorods by deprotonation of poly(aminophenylboronic acid) (PABA) coating. Boronated ester bonding and hydrogen-bonds at CNCs/PABA interface collectively strengthen dipole moments while inducing n-type doping in PABA and thus enabling charge transport for 18.9-fold conductivity. Integrating molecular-level interfacial alignment of conjugated moieties, CNCs with higher aspect-ratio further promote percolation and reduce threshold by ~14%. The charge transport range in hydrogel is then elongated, which improves its conductivity by 7.0 times and yields robust piezo-electric/resistive dual-mode responsiveness. The hydrogen-bond network also improves adhesion, and imparts intrinsic sensitivity to temperature, salinity, and pH, for distinguishing complex motions and physiological events. This heterojunction-driven co-engineering of dipoles and conductivity offers a generalizable materials concept for advancing high-fidelity soft bioelectronic sensing.
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Biomolecules
·
2025
·Vol. 15
·期 6
·822
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IF 4.8
Xiabin Chen,Yishuang Li,Jianzhuang Yao,Xiaoxuan Li,Hualing Li,Zelin Wu,Qi Hu,Nuo Xu,Tingjun Hou,Jiye Wang,Shurong Hou
6-monoacetylmorphine (6-MAM), a primary active metabolite of heroin that reaches the human brain, plays a crucial role in producing heroin-associated physiological and lethal effects. Therefore, 6-MAM has emerged as a key target for alleviating the adverse consequences of heroin abuse. In this study, the proposed 6-MAM hydrolaseE. coliaminopeptidase N (eAPN) was recombinantly produced, and its biochemical and functional profiles were investigated. eAPN’s biochemical properties, with respect to pH, metal ions, and temperature, and catalytic functions toward peptidase substrates and 6-MAM were thoroughly examined. Extensive experiments reveal that incorporation of an N-terminal His-tag notably affects eAPN’s aminopeptidase activity. This cost-effective recombinant eAPN exhibits favorable thermostability and optimal activity at pH 7.5. Kinetic analysis toward peptidase substrates reveals that eAPN preferentially cleaves peptides following amino acid residues in the order of Ala > Arg >> Met, Gly > Leu > Pro, indicating a preference for small or basic amino acid residues as substrates. Computational and experimental studies have, for the first time, discovered that eAPN is capable of catalyzing the hydrolysis of heroin and 6-MAM, which has shed light on its functional versatility and potential applications. This work elucidates the biochemical properties of eAPN and expands its catalytic functions, thereby laying the groundwork for a deep understanding and further reengineering of eAPN to enhance its activity toward 6-MAM for heroin detoxification.
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APPLIED BIOCHEMISTRY AND MICROBIOLOGY
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2025
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IF 1.1
Wu S. J.,Chen F.,Ma X. J.,Sun S. Y.,Yan H. D.,Li T. T.,Qiu L. Q.
( R )-citronellal is a key intermediate in the synthesis of L-menthol, one of the world’s three most important spices. It can be obtained by asymmetric reduction of ( E / Z )-citral using old yellow enzymes (OYEs), but catalysis by the wild-type enzyme suffers from poor enantioselectivity as well as low efficient conversion. A protein-directed evolution technique was used to enhance the activity and enantioselectivity of Ct OYE from Chroococcidiopsis thermalis . Upon the sequence alignment and molecular docking results, two amino acid residues at positions 103 and 351 were selected as saturation mutation sites. Finally, three single substitution variants of Ct OYE-W103E, Ct OYE-W103M, Ct OYE-W103P with improved enantioselectivity ( ee > 99%) and moderate conversion (>71%), and one double substitution variant Ct OYE-Y351Q/W103E with high enantioselectivity ( ee > 99%) and high conversion (83.2%) for reduction of ( E / Z )-citral were obtained.
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JOURNAL OF COMPOSITE MATERIALS
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2026
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IF 2.9
Abuzar Es’haghi Oskui,Elyas Haddadi
The influence of tailoring the carrier-fluid chemistry of shear-thickening fluids (STFs) on the dynamic response of three-dimensional (3D) E-glass fabric laminates is investigated. Silica-based STFs (50 wt%) were formulated in polyethylene glycol (PEG-400), and the PEG was further modified using diacid chemistry (oxalic and glutaric) to tune chain interactions and hydrogen-bonding density. Fabrics were impregnated via deep coating and the add-on was quantified. Rheology, surface morphology (SEM), yarn pull-out response, and low-velocity drop-weight compression were evaluated for 1-, 3-, 5-, and 7-ply stacks. Post-critical viscosity was increased by PEG modification, and larger apparent particle agglomerates were observed within yarn interstices relative to the unmodified STF, indicating strengthened fabric–fluid–particle interlocking. During yarn pull-out, inter-yarn friction and energy absorption were increased; relative to untreated E-glass, energy absorption gains of ≈25.95%, 31.08%, and 61.38% were obtained for pure-STF, oxalic-modified, and glutaric-modified STF composites, respectively. Under compressive impact, STF impregnation reduced the compressive peak force (CPF) and extended the force-duration window; CPF ratios of ∼0.85, 0.76, and 0.65 (relative to untreated fabric) were measured for pure-, oxalic-, and glutaric-modified STFs, with further improvements as ply count increased, while peak-force time and impulse duration were systematically shifted to later times. To interpret the cushioning dynamics, a compact regression model combining a Gaussian peak term (capturing peak force and timing) with linear components was established; material-specific parameterizations were shown to reproduce measured force–time histories and to enable prediction across ply counts. Overall, scalable improvements in energy dissipation, inter-yarn friction, and impact cushioning were achieved through modest PEG-chemistry modifications, enabling tunable protection for diverse engineering and armor applications.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Xianghua Wang,Maorun Fu,Xiaoqian Qu,Jingjing Liu,Jianwen Bu,Shaoran Feng,Handong Zhao,Wenxiao Jiao,Fei Sun
(E)-2-Hexenal is one of the most prevalent and abundant volatile substances in fruits. Many researchers have attempted fumigation with (E)-2-hexenal to control fungal diseases with poor or no results; the key is the inability to maintain an effective concentration for a long time. Therefore, a new starch-based antifungal coating with (E)-2-hexenal microcapsules was prepared. The obtained results showed that the microcapsule embedding rate reached 78.95% by orthogonal optimization experiments and that the (E)-2-hexenal-based coating was effective at reducing the lesion diameter of apple fruit during storage at 25 °C after Penicillium expansum infection, and in other fruits; it also achieved a good antifungal effect. Moreover, the activities of the main defense-related enzymes and the expression of key genes involved in the salicylic acid (SA) signaling pathway, such as CHI, GLU, PAL , NPR1 , and PR1 , were enhanced by (E)-2-hexenal-based coating treatment. These findings suggest that the (E)-2-hexenal-based coating has the dual effect of direct antifungal and induction of apple system resistance. E2H-based coating is a promising approach to control postharvest Penicillium expansum in fruit.
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JOURNAL OF CONTROLLED RELEASE
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2021
·
IF 9.776
Shanshan Zhang,Sajid Asghar,Chenqi Zhu,Junxiu Ye,Ling Lin,Liu Xu,Ziyi Hu,Zhipeng Chen,Feng Shao,Yanyu Xiao
Targeting a single molecule or a single pathway and poor drug delivery to the brain hamper the therapy of Alzheimer's disease (AD) based on abnormal metabolism of amyloid-β (Aβ). To solve these problems, we designed and synthesized a multi - strategy peptide (MOP), an ingenious apolipoprotein E mimetic peptide, which could reduce Aβ deposition via inhibiting Aβ aggregation and at the same time accelerate Aβ clearance. Meanwhile, MOP could be self-assembled into different nanostructure , thus we constructed a multifunctional delivery system (APND-3) based on MOP self-assembled nanorods (aspect ratios of 3) that was a favorable morphology to enhance the permeation across the blood brain barrier (BBB) to address the poor delivery to brain issues. Besides, the drug delivery system introduces polydopamine (PDA) and COG1410 ligand as a shell to keep the favorable morphology of core and enhance the BBB targeting efficiency. As a result, the delivery system significantly enhances the delivery of MOP to the brain, thus reducing Aβ deposition, mitigating the memory deficits, and ameliorating neurologic damage in AD model mice. Our findings suggest that our drug and carrier integrated multifunctional delivery system has the potential for AD treatment.
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CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 18
·期 5
·e2100128
·
IF 2.408
Shu-Hua Zhang,Jiewei Yang,Hao Ma,Yu Yang,Guo-Feng Zhou,Xue Zhao,Rui Xu,Dan Nie,Guo-Gang Zhang,Jun-Jie Shan,Cheng-Bin Cui,Chang-Wei Li
Five new peptaibols, longibramides A–E ( 1 – 5 ) with 11 amino acid residues, were isolated from a fungus Trichoderma longibrachiatum Rifai DMG-3-1-1, which was isolated from a mushroom Clitocybe nebularis (Batsch) P. Kumm collected from coniferous forest in the subboreal area of northeast China. The structures of longibramides A–E were determined by their spectroscopic data (NMR and MS-MS spectra), their absolute configurations were determined by X-ray diffractions and Marfey's analyses. The X-ray diffractions of longibramides A, B, and the similar CD spectra of A–E showed that they all had α -helix conformations. Longibramides B and E showed moderate cytotoxicities against BV2 and MCF-7 cells and also showed some inhibitory effects against methicillin-resistant Staphylococcus aureus MRSA T144. L- trans- Hyp was not commonly found in natural peptaibols, which was the 6 th or 10 th amino acid residue in longibramides C–E. The X-ray diffractions of longibramides A and B afforded the accuracy conformations of their secondary structures, which maybe help to interpret the structure-activity relationships of the family of peptaibols in the future. Graphical
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JOURNAL OF PHARMACEUTICAL SCIENCES
·
2020
·
IF 2.997
Zipeng Huang,Xinru Chen,Ying Li,Junqiu Zhai,Yan Ma
Curcumin (CUR) has attracted wide research interests due to its abundant bioactivities and potential advantages in cancer treatment. But the poor water solubility, instability, and quick metabolization and elimination after oral administration severely restrict the efficacy and further clinical application of CUR. Derivation is an approach often used to improve the druggability of active ingredients, so the study aim to prepare a CUR derivate with better stability, satisfactory pharmacokinetics , and inherent self-assembled ability in contrast with CUR. The derivate was designed and evaluated in vitro and in vivo . Vitamin E (VE) was used to perform the esterification reaction with CUR, and the cytotoxicity of derivative CUR-VE ester on MCF-7 tumor cells was similar to CUR. Besides the better stability in simulated gastric and intestinal fluid, plasma and liver homogenate, the self-assembly CUR-VE nanoparticles were fabricated by feasible and controllable nanoprecipitation method. The Transmission Electron Microscope (TEM) showed CUR-VE NPs were spherical with an average particle size of 172.9 nm , and drug loading was up to 93%. CUR-VE NPs exhibited a sustained-release behavior and fitted to Fick's diffusion mechanism. Differential Scanning Calorimeter (DSC), X-ray Powder Diffractometer (XRPD) and Fourier Transform Infrared Spectrometer (FTIR) declared no crystalline substances were formed, and the self-assembly process of CUR-VE relied on driving forces including van der Waals forces , hydrogen bonding forces, intermolecular forces . In pharmacokinetics, C max and AUC 0-∞ of CUR-VE NPs by the route of oral administration were (104.69 ± 40.72) ng/mL and (3496.92 ± 1088.93) ng/mL∗h, which were about three times and 18 times more compared with CUR. The eliminated half-time of CUR-VE extended to 28 h ascribed to the outstanding stability and surface PEGylation of NPs. It prompted that appropriately PEGylated NPs via oral administration was beneficial to prolong systemic circulation similar to intravenous PEGylated NPs. In summary, the study provides a convenient way to fabricate the self-assembled CUR-VE NPs qualified with high drug loading, satisfactory stability and desired pharmacokinetics. CUR-VE has the potent advantage to be an ideal substitute for CUR in the future of healthcare and clinical application.
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