PARASITOLOGY RESEARCH
·
2022
·Vol. 121
·期 2
·675-689
·
IF 2.383
Haseeb Muhammad,Huang Jianmei,Lakho Shakeel Ahmed,Yang Zhang,Hasan Muhammad Waqqas,Ehsan Muhammad,Aleem Muhammad Tahir,Memon Muhammad Ali,Ali Haider,Song Xiaokai,Yan Ruofeng,Xu Lixin,Li Xiangrui
Eimeria maxima (E. maxima) are an intracellular apicomplexan protozoan that causes intestinal coccidiosis in chickens. The purpose of this research was to develop a novel delivery approach for recombinant E. maxima (rEm) 14-3-3 antigen to elicit enhanced immunogenic protection using poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NPs) against E. maxima challenge. The morphologies of prepared antigen-loaded NPs (PLGA/CS-rEm14-3-3 NPs) were visualized by a scanning electron microscope. The rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs–immunized chicken-induced changes of serum cytokines, IgY-antibody level, and T-lymphocyte subsets and protective efficacies against E. maxima challenge were evaluated. The results revealed that encapsulated rEm14-3-3 in PLGA and CS NPs presented spherical morphology with a smooth surface. The chickens immunized with only rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs elicited a significant ( p <0.05) higher level of IFN-γ cytokine, stimulated the proportions of CD4 + /CD3 + , CD8 + /CD3 + T-cells, and provoked sera IgY-antibody immune response compared to control groups (PBS, pET-32a, PLGA, and CS). Whereas, PLGA-rEm14-3-3 NP–immunized chicken provoked a higher level of IFN- γ production and IgY-antibody response rather than CS-rEm14-3-3 and bare antigen, relatively. The animal experiment results ratified that PLGA-rEm14-3-3 NP–immunized chicken significantly alleviated the relative body weight gain (%), decreased lesion score, and enhanced oocyst decrease ratio compared to CS-rEm14-3-3 NPs and only rEm14-3-3. The anti-coccidial index of the chicken vaccinated with the PLGA-rEm14-3-3 NPs was (180.1) higher than that of the Cs-rEm14-3-3 NPs (167.4) and bare antigen (165.9). Collectively, our statistics approved that PLGA NPs might be an efficient antigen carrier system (Em14-3-3) to act as a nanosubunit vaccine that can improve protective efficacies in chicken against E. maxima challenge.
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高影响因子
INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Jianlong Huang,Fengjiao Guo,Zhiyong Guo,Jianbang Chen,Bin Dai,Feng Yu
A series of ammonium-containing fluoroiodates, NH4IO2F2 and (NH4)3(IO2F2)3·H2O, with isolated [IO2F2] units have been fabricated by a fluorine-oxygen substitution strategy from NH4IO3. The two compounds crystallize in the orthorhombic system, but in different space groups, noncentrosymmetric Pca21 for NH4IO2F2 and centrosymmetric Pnma for (NH4)3(IO2F2)3·H2O, and show wide band gaps of 4.53 eV for (NH4IO2F2) and 4.55 eV for ((NH4)3(IO2F2)3·H2O). In addition, NH4IO2F2 exhibits a 1.2 × KDP second harmonic generation response, a short ultraviolet cutoff edge in iodates, and a good crystal growth habit. The crystal of NH4IO2F2 with a size of 11 × 5 × 2 mm3 was obtained by the aqueous solution method. The results enrich the structural diversity of iodate and supply a greater understanding of the design of new functional materials based on the fluoroiodates.
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高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 139
·期 4
·51551
·
IF 3.125
You Wu,Yujuan Jin,Jian Huang,Huafeng Tian,Yunxuan Weng
Phenyl terminated hyperbranched polyesters (HBP-Bs) were synthesized based on Boltorn-type hydroxyl terminated HBPEs and characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, gel permeation chromatography, and so forth. The prepared HBP-Bs were used to toughen poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) directly by melt blending. The thermal properties, rheological properties, mechanical properties, and microstructure of the PHBV/HBP-Bs blends were studied in detail. Comparing with pure PHBV, the crystallinity of the PHBV/0.5 phr HBP-Bs blends decreased by 14.10% (from 64.39% to 55.04%), the impact strength increased by 158.30% (from 7.41 kJ·m −2 to 19.14 kJ·m −2 ), and the elongation at break increased by 400.00% (from 0.91% to 4.55%). It can be seen from the scanning electron microscope that the fracture surface of the PHBV/HBP-Bs blends became rough with the increase of HBP-Bs, even with large filamentous structures and tiny holes, which further demonstrated that HBP-Bs acted as an excellent toughening effect on PHBV.
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MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Mengling Lin,Yingjie Guo,Zhiyong Liang,Xuesong Zhao,Jianan Chen,Yilin Wang
Biothiols, generally including glutathione (GSH), cysteine (CySH), and homocysteine (HcySH), are important biomarkers and closely related to many diseases. In this work, a simple method for the spectrophotometric determination of the three biothiols was developed. The proposed method was based on the fact that Fe 3+ could oxidize 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to oxidized TMB (ox-TMB) and induce a blue color solution corresponding to an absorption peak centered at 652 nm. However, the introduction of a biothiol compound could cause the reduction of ox-TMB which resulted in a blue color fading and a decrease of the absorbance. Based on this finding, we developed a method for the determination of biothiols with the help of UV–vis spectroscopy, and the influence of different factors including pH, temperature, and incubation time was studied when glutathione worked as an example. The detection limits for GSH, CySH, and HcySH were 1.04, 0.82, and 2.09 μM, respectively. More importantly, the suggested method was successfully applied for the determination of GSH in human serum samples with the recovery of 97%–112%. This method is simple, fast and without the use of complicated nanomaterials .
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CHINESE CHEMICAL LETTERS
·
2014
·
IF 1.178
Hu-Yan Li,He Li,Bing-Jie Wang,Qun Gu,Zhi-Qiang Jiang,Xue-Dong Wu
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/chitin nanocrystals (CNC) composite scaffolds were synthesized by the salt leaching and thermally induced phase separation (TIPS) technique. The scaffolds have porous structures with macro-pores (100–300 μm in diameters) and micro-pores (10 μm). The surface characteristics of the scaffolds were characterized by X-ray photoelectron spectroscopy (XPS) and static water contact angle measurement, and the mechanical properties were investigated by a compression test. Human adipose-derived stem cells (hADSCs) were seeded onto the PHBV/CNC scaffolds and in vitro cell culture results showed that the composite scaffolds enhanced the hADSCs adhesion, which implies that the material may have potential application in tissue engineering.
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高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2013
·
IF 3.458
Xiaomin Yang,Lin Zhao,Laszlo Almasy,Vasil M. Garamus,Aihua Zou,Regine Willumeit,Saijun Fan
Chemically modified tetracyclines (CMTs) have been reported to strongly inhibit proliferation and metastasis of various cancers, but their efficacy is restricted by poor water solubility. In the present study, a hydrophilic 4-dedimethylamino sancycline (CMT-3) loaded nanostructured lipid carrier (CMT-3/NLC) was produced by high pressure homogenization (HPH). The physical properties of CMT-3/NLC formulations were characterized by dynamic light scattering (DLS), high efficiency liquid chromatography (HPLC), atomic force microscopy (AFM), scanning electron microscopy (SEM), small-angle neutron scattering (SANS), small-angle X-ray scattering (SAXS) and wide-angle X-ray powder diffraction (XRD). The lipid and surfactant ingredients, as well as drug/lipid concentrations (m/m) were optimized to produce stable and sustained NLC formulations. In vitro cytotoxicity of CMT-3/NLC against HeLa cells was evaluated by MTT assay. The diameter of CMT-3/NLC was found to increase from 153.1 ± 3.0 nm to a maximum of 168.5 ± 2.0 nm after 30 days of storage, while the entrapment efficiency remained constant at >90%. CMT-3/NLC demonstrated a burst-sustained release profile in release media with different pH, a property attributed to the 3-dimensional structure of CMT-3/NLC. Cell uptake and localization studies indicated that NLC reached the cytoplasm and could thereby facilitate CMT-3 entry into HeLa cells.
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高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2022
·
IF 4.198
Xiang Liu,Yuhan Wang,Huitao Zheng,Junyan Huang,Zhengquan Su,Limin Zhao,Hua Cao
Here, we report a facile and metal-free method for the construction of dihydrooxazine derivatives via a formal (3 + 3) annulation reaction of naphthols and 1,3,5-triazinanes. The 1,3,5-triazinanes were utilized as a formal three-atom synthon (C–N–C) for cycloaddition. In addition, dihydrothiazine and tetrahydrobenzoquinazoline derivatives could also be produced in good yields by this strategy under catalyst-free and additive-free conditions.
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高影响因子
ANALYST
·
2019
·Vol. 145
·期 3
·1025-1032
·
IF 4.019
Hui Xu,Zijie Feng,Yingxiang Du
Recently, ionic liquids have been widely used for chiral separation. However, several papers have reported the use of ionic liquids as the sole chiral selector. In this work, a novel capillary electrophoresis method using the ionic liquid 3-methyl-1-(3-sulfopropyl)-1H-imidazol-3-ium lactobionate (MSI-LA) as a chiral selector was developed for the enantioseparation of propranolol, metoprolol, bisoprolol, esmolol, atenolol, sotalol, terbutaline and clenbuterol. Method optimization was conducted and the optimized method (40 mM borax buffer, v/v 30% methanol, pH 8.5, 160 mM MSI-LA; +10 kV voltage; capillary temperature, 15 °C; 50 mbar/5 s injection; detection wavelength, 230 nm) was successful for the baseline separation of the eight basic drugs. The LA (lactobionic acid) system was compared. The possible mechanisms of enantioseparation were investigated using molecular modeling. Both the cation and anion of MSI-LA provided interactions between the chiral selector and analytes, and finally enhanced chiral recognition capability compared with LA.
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高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Guiyin Li,Bo Wang,Le Zhao,Xiaohang Shi,Guanxiong Wu,Wei Chen,Lixian Sun,Jintao Liang,Zhide Zhou
Glypican-3 (GPC3) protein is considered as an effective and specific biomarker for the accurate diagnosis of hepatocellular carcinoma (HCC), and there is a critical demand to construct strategies with shorter time, lower cost noise and higher sensitivity. In this study, a biosensor for label-free detection of GPC3 was prepared by modifying GPC3 aptamer (GPC3 Apt ) on the surface of Si-based light addressable potentiometric sensor (LAPS) chip modified by reduced graphene oxide/polyetherimide/gold nanoparticles (rGO/PEI/AuNPs). Among them, GPC3 Apt with a low equilibrium dissociation constant was used as a biorecognition molecule to recognize and capture GPC3. The rGO/PEI/AuNPs decorated on the LAPS surface could enlarge the surface area for GPC3 Apt immobilization and provide more active sites for GPC3 Apt to bind effectively with the target GPC3. When a bias voltage was provided to the LAPS system, the forming GPC3-GPC3 Apt complexs on the sensing interface of LAPS would cause the potential of the sensitive membrane surface to change and generate a corresponding offset response in photocurrent-voltage (I-V) curves. The potential shift and GPC3 concentration showed a good linear range from 0.1 to 100.0 μg/mL with a detection limit of 40.0 ng/mL (S/ N = 3) and a sensitivity of 1.33638 mV/μg/mL. In addition, the LAPS detection system also has good specificity and stability, which can be used for the detection of GPC3 in spiked serum samples.
研究领域:
生物传感器
·
癌症诊断
·
纳米技术
·
分析化学
·
生物医学工程
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高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Hui Sun,Baoyang Lu,Xuemin Duan,Jingkun Xu,Liqi Dong,Xiaofei Zhu,Kaixin Zhang,Dufen Hu,Shouli Ming
The copolymerization of 2’-aminomethyl-3, 4-ethylenedioxythiophene (EDOT-MeNH 2 ) and 3, 4-ethylenedioxythiophene (EDOT) was successfully performed electrochemically in acetonitrile (CH 3 CN) containing tetrabutylammonium hexafluorophosphate (Bu 4 NPF 6 ) as the supporting electrolyte by direct anodic oxidation of the monomer mixtures. A series of experiments with different monomer feed ratios were carried out to investigate the influence of monomer feed ratio on the electrochemical copolymerization and the overall properties of the copolymer films. As-formed novel copolymers owned both the advantages of poly(2’-aminomethyl-3, 4-ethylenedioxythiophene) (PEDOT-MeNH 2 ) and poly(3, 4-ethylenedioxythiophene) (PEDOT), i.e ., good electrochemical behavior, excellent electrochemical stability, and good thermal stability. Spectroelectrochemistry analysis of copolymer with the feed ratio of 1:1 reflected λ max due to the π–π* transition and polaron band formation was found to be 620 nm and the band gap ( E g ) was calculated as 1.67 eV. The structure, thermogravimetry and morphology of the copolymers were investigated by infrared spectroscopy, thermal analysis, and scanning electron microscopy, respectively.
研究领域:
材料科学
·
高分子化学
·
电化学
·
导电聚合物
·
有机电子学
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高影响因子
CERAMICS INTERNATIONAL
·
2020
·Vol. 46
·期 10
·16524-16532
·
IF 3.83
Muhammad Yousaf,Asma Noor,Shuai Xu,Majid Niaz Akhtar,Baoyuan Wang
Yttrium iron garnet (YIG) nanoparticles (NPs) doped with rare earth (RE) metal ions (Y 2.5 Sm 0.5 Fe 5 O 12 , Y 2.5 Nd 0.5 Fe 5 O 12 ) were successfully synthesized by sol-gel auto combustion approach. The cubic crystalline structure and morphology of the prepared garnet ferrite NPs were analyzed by X-ray diffractometer (XRD) and field emission scanning electron microscopy (FESEM). The cubic crystalline garnet phase of the synthesized YIG, Sm-YIG and Nd-YIG samples was successfully achieved at 950 °C sintering temperature . The force constant and absorption bands were estimated by using Fourier transform infrared spectroscopy (FTIR). The doping effect of RE metal ions on the chemical states of YIG were examined by x-ray photoelectron microscopy (XPS). The valence band (from 12.63 eV to 13.22 eV), conduction band (from 10.89 eV to 11.34 eV) edges and optical bandgap values of RE doped YIG samples were calculated using UV–Vis spectroscopy and ultraviolet photo electron spectroscopy (UPS). The magnetic analysis of the prepared NPs was studied using vibrating sample magnetometer (VSM). The XPS analysis of RE doped YIG samples exhibit the existence of RE (Sm +3 , Nd +3 ) contents on the surface of YIG ferrite by decreasing the oxygen lattice in garnet structure. The optical bandgap (from 1.74 eV to 1.88 eV) explains the semiconducting nature of the synthesized NPs. The UPS results confirm the valence band position of YIG doped samples. The saturation magnetization and remanence of RE doped garnet ferrite samples increased from 13.45 to 18.83 emu/g and 4.06–6.53 emu/g, respectively.
研究领域:
材料科学
·
纳米科技
·
固体物理
·
无机化学
·
功能材料
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高影响因子
CARBOHYDRATE POLYMERS
·
2013
·
IF 3.479
Bingjie Wang,Jun Li,Jianqiang Zhang,Huyan Li,Peng Chen,Qun Gu,Zongbao Wang
Acetylated chitin nanocrystals were prepared through surface modification, and biodegradable poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/chitin nanocrystals films were produced via solution-casting method. Transmission electron microscopy observations and X-ray diffraction profiles revealed that the rod-like morphology and crystal structure of chitin nanocrystals were maintained. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy results showed that the hydroxyl groups were partly replaced by the acetyl groups on the surface of chitin nanocrystals. The hydrophobic performance of the acetylated chitin nanocrystals was significantly increased according to the contact angle measurements. Differential scanning calorimeter results indicated that the influence of chitin nanocrystals on the crystallization behaviors of PHBV matrix was changed from suppression to assistance after the surface modification. Tensile test showed that the tensile strength and Young's modulus of PHBV/acetylated chitin nanocrystals composites were improved by 44% and 67%, comparing to the improvement of 24% and 43% for PHBV/chitin nanocrystals composites with the addition of 5.0 wt.% nanocrystals into PHBV.
研究领域:
材料科学
·
生物医学工程
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高影响因子
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