Applied Materials Today
·
2026
·
IF 7
Siqi Zhan,Chihao Gao,Junyi Yan,Xiaoli Lin,Xu Yan,Yaohui Fan,Zongrui Zhang,Xinyu Wang
Improve the sterile inflammation caused by soft tissue fillers. • Reduce inflammatory mediators and ROS levels. • Promoted cell migration and orderly collagen deposition. The increasing prevalence of soft tissue fillers has given rise to a concern regarding the occurrence of local sterile inflammation induced by the filler material. This study proposes the use of Poly(D,L-lactic acid) (PDLLA) microspheres composite with D-α-Tocopherol acetate (α-TA) and Hydroxyapatite (HAp) to eliminate aseptic inflammation caused by PDLLA degradation. The reduction of aseptic inflammation is achieved by prolonging degradation time, modulating the local microenvironment, and suppressing inflammatory mediators. HAp and α-TA were evenly distributed in PDLLA/HAp(α-TA) microspheres, and those microspheres have a particle size suitable for soft tissue filling (30–70 µm). Utilizing a conventional commercial formulation, PDLLA/HAp(α-TA) is uniformly amalgamated with carboxymethyl cellulose (CMC) to formulate CMC@PDLLA/HAp(α-TA) gel. CMC@PDLLA/HAp(α-TA) exhibits sustained release properties for at least 6 weeks and possesses excellent injectability. Meanwhile, CMC@PDLLA/HAp(α-TA) exhibits excellent biocompatibility and the ability to promote cell migration. Specifically, CMC@PDLLA/HAp(α-TA) significantly reduces inflammatory mediators and reactive ROS levels, while exhibiting prolonged and orderly collagen deposition. In summary, as an injectable soft tissue filler, CMC@PDLLA/HAp(α-TA) offers a novel strategy for the long-term suppression of sterile inflammation. Download: Download high-res image (287KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2026
·
IF 5.7
Junjie Huang,Jiahao Zhu,Huijun Zheng,Enhui Zhu,Chenqi Yang,Liqin Ying,Yucong Zhou,Hao Liu
The neuroprotective effect of DAAOi is associated with increased levels of its substrate, D-Serine. • D-Serine can ameliorate cognitive and behavioral deficits in MCAO rats. • D-Serine may exert its neuroprotective effect by promoting the D-Serine/GluN2A/BDNF signaling pathway. To investigate the mechanisms of D-Amino acid oxidase inhibitor (DAAOi) in ameliorating cerebral ischemia-reperfusion (I/R) injury and the underlying neuroprotective effect of substrate D-Serine. A middle cerebral artery occlusion (MCAO) rat model and an oxygen-glucose deprivation (OGD)-induced HT22 cell model were used to mimic ischemic stroke. The modified Neurological Severity Scores (mNSS) and 2, 3, 5-triphenyltetrazolium chloride staining were applied to assess neurological behavioral scores and cerebral infarct area, respectively. The effects of DAAOi 4H-Furo[3,2-b]pyrrole-5- carboxylic acid (SUN) and D-Serine intervention on cognitive and behavioral functions in MCAO rats were evaluated by using the Y-maze and Novel Object Recognition Test (NORT). Intracerebral stereotaxic injection of Compound SUN and D-Serine (0.21 mg, 1.05 mg, 2.1 mg) significantly reduced cerebral infarct area, indicating that DAAOi might improve cerebral I/R injury by elevating D-Serine levels. Similarly, D-Serine significantly enhanced cognitive and behavioral performance in MCAO rats (mNSS: improved by 2.3 scores; Y-maze: spontaneous alternation rate increased by 14.76%; NORT: recognition index improved by 12.28%). D-Serine pretreatment (10 μM-10 mM) dose-dependently increased the viability of HT22 cells subjected to OGD. In both in vitro and in vivo models, D-Serine significantly increased the protein level of N-methyl-D-aspartate receptor subunit 2A (GluN2A). Additionally, D-Serine promoted the expression of brain-derived neurotrophic factor (BDNF) and postsynaptic density protein-95 but inhibited the apoptosis-related p38 MAPK phosphorylation. This study demonstrated that the neuroprotective effect of DAAOi was at least partly mediated by elevating cerebral D-Serine levels, which regulated the GluN2A/BDNF axis to enhance synaptic plasticity and cognitive function. Download: Download high-res image (323KB) Download: Download full-size image
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Yue Tang,Xin Ju,Yuqi Peng,Zhicheng Xie,Wei Liu,Jiaolong Fu,Jiajia Chen,Liangzhi Li
D-Arabitol is a naturally occurring pentitol widely used as a low-calorie sweetener and functional ingredient in food formulations. However, microbial production from biomass-derived D-xylose remains constrained by low product titers, limited conversion yields, and the lack of efficient engineered xylose-utilizing strains. In this study, the osmotolerant yeast Trichosporonoides oedocephalis was engineered to produce D-arabitol from D-xylose through the introduction of a heterologous XI (xylose isomerase)-ArDH (arabitol dehydrogenase) pathway. Fermentation conditions relevant to high-sugar food systems were systematically optimized. Compared with the addition of 0.5 g/L KCl (yielding 17.69 g/L D-arabitol), supplementation with 30 g/L KCl significantly increased D-arabitol production, reaching 35.13 g/L in shake flasks and 43.91 g/L in a 5-L bioreactor. Semi-batch fermentation further increased the final titer to 55.21 g/L with a yield of 0.280 g/g. Kinetic analysis indicated predominantly growth-associated D-arabitol formation under these conditions (Luedeking-Piret coefficient, α = 0.61). Comparative transcriptomic analysis revealed that KCl supplementation induced phase-dependent physiological responses, including enrichment of nucleotide metabolism and coordinated transcriptional regulation of central carbon metabolism during exponential growth, as well as increased expression of sugar transport and protein maintenance pathways during the stationary phase. These changes were accompanied by elevated activities of xylose isomerase and arabitol dehydrogenase, suggesting that salt stress is associated with efficient carbon utilization and the biosynthesis of polyols. Importantly, KCl represents a food-compatible and physiologically relevant process regulator, highlighting the potential of salt-mediated metabolic regulation for food-related polyol fermentations. These findings provide an effective strategy for food-related D-arabitol production from xylose and offer insights into salt-associated metabolic regulation in yeast.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2023
·
IF 7.2
Yibin Yang,Di Gao,Yingqing Ou,Yang Yang,Peng Xiao,Yunhuai Zhang
Currently, developing highly active and low-cost electrocatalytic materials for oxygen evolution reaction (OER) is an enormously grand challenge. Herein, we developed a novel and highly active Mn doped Co 2 Fe layer double hydroxide (LDH) electrocatalyst for OER. We discovered that these electrocatalytic materials can be directly grown on carbon papers to construct high-specific-surface-area electrode, which shows the lowest overpotential of 266 mV at 10 mA cm −2 . Furthermore, after introducing Mn element, DFT + U calculation found that the ∗OOH of Fe-site on Co 2 Fe 0.67 Mn 0.33 LDH could draw more electrons than Co 2 Fe LDH due to the electronegativity differences between Fe-site on Co 2 Fe 0.67 Mn 0.33 LDH and Fe-site on Co 2 Fe LDH, which is reason that the energy level of Fe 3d (e g ) was obviously downshifted by d-d repulsion of Mn 3d and Fe 3d in the neighboring sites of Co 2 Fe 0.67 Mn 0.33 LDH after doped Mn element, which leads to reduce charge-transfer energy from O 2p to Fe 3d (e g ) to promote oxygen evolution processes for OER. Meanwhile, the band gap is also decreased after doped Mn element in Co 2 Fe LDH due to the downshifted e g orbital energy of Fe 3d. This study gives a general avenue to design and developing efficiently active LDH electrocatalysts for OER in the future.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 103
·期 2
·793-806
·
IF 3.34
Yang Haiquan,Hu Jinyuan,Lu Xiao,Wang Fuxiang,Shen Wei,Hu Wei,Wang Lingling,Chen Xianzhong,Liu Long
Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli , for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli cell wall by overexpressing D,D-carboxypeptidase genes dacA or dacB , and investigated the effect on extracellular protein production. Overexpression of dacA or dacB promoted the accumulation of intracellular soluble peptidoglycan, altered cell morphology (shape and size) and led to the formation of transparent globular structures in E. coli cells. Compared with controls (CK), extracellular production of recombinant green fluorescent protein (GFP) was increased by 1.7- and 2.3-fold upon overexpression of dacA and dacB , respectively. Similarly, extracellular production of recombinant amylase and α-galactosidase was increased by 4.5- and 2.8-fold, respectively, upon overexpression of dacA , and by 11.9- and 2.5-fold, respectively, upon overexpression of dacB . Overexpression of dacA or dacB enhanced both the outer and inner membrane permeability of E. coli . This cell wall engineering strategy opens up a new direction for enhancing extracellular protein and chemical production in E. coli .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Theranostics
·
2018
·
IF 8.537
Zhenyuan Bian,Jin Yan,Simeng Wang,Yijie Li,Yi Guo,Bohan Ma,Hao Guo,Zhijie Lei,Chun Yin,Yi Zhou,Min Liu,Kaishan Tao,Peng Hou,Wangxiao He
Peptides are a rapidly growing class of therapeutics with many advantages over conventional small molecule drugs. Dextrorotary (D)-peptides, with increased enzymatic stability and prolonged plasma half-life in comparison with natural L-peptides, are considered to have great potential as recognition molecules and therapeutic agents. However, the in vivo efficacy of current therapeutic D-peptides is hindered by their inefficient cellular uptake in diseased tissues. Methods: To overcome physiological and cellular barriers to D-peptides, we designed a gold-based ultra-small nanocarrier coupled with polylysine (PLL) and a receptor-targeted peptide to deliver therapeutic D-peptides. Using a D-peptide p53 activator (DPA) as a proof of concept, we synthesized, functionalized and characterized gold- and DPA-based nanoparticles termed AuNP-DPA. Results: AuNP-DPA were effectively enriched in tumor sites and subsequently internalized by cancer cells, thereby suppressing tumor growth via reactivating p53 signaling. More importantly, through a series of in vivo experiments, AuNP-DPA showed excellent biosafety without the common side effects that hinder p53 therapies in clinic trials. Conclusion: The present study not only sheds light on the development of AuNP-DPA as a novel class of antitumor agents for drugging the p53 pathway in vivo, but also supplies a new strategy to use D-peptides as intracellular PPI inhibitors for cancer-targeted therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2022
·
IF 3.318
Hua Li,Yang Wang,Ru Ding,Chen Chen,Zhixuan Tian,Hongnan Yin,Lieming Ding
A sensitive and reliable method was developed to determine befotertinib (D-0316) and its metabolite D-0865 from human plasma by LC-MS/MS. The samples were prepared by simple protein precipitation and 2 µL of the supernatant were chromatographed on a C18 analytical column (ACE Excel 2 Super C 18 , 50 × 2.1 mm). Elution was performed with mobile phase A (10 mM ammonium acetate in water containing 1 % formic acid) and mobile phase B (acetonitrile containing 1 % formic acid) under a gradient program in a total run time of 4 min. Triple Quadruple 5500 equipped with Turbo Ion Spray source and multiple reaction monitoring (MRM) were used for the analysis detection. The transitions were m / z 568.3 → 72.1 m / z (befotertinib), m / z 554.2 → 497.2 (D-0865), and m / z 455.2 → 164.9 (verapamil, internal standard). According to the Chinese Pharmacopeia Commission and ICH Harmonised Guideline for Bioanalytical Method Validation, this method was validated within the spectrum of its accuracy, precision, selectivity, linearity, recovery, matrix effect, and stability. This LC-MS/MS method was successfully applied for the quantitation of befotertinib and its metabolite D-0865 in human plasma during the pharmacokinetics study of befotertinib in non-small cell lung cancer (NSCLC) patients.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2022
·
IF 3.858
Jianjiong Li,Shanshan Yu,Yingang Wang,Peiyuan Yao,Qiaqing Wu,Dunming Zhu
(S)-2-Aminobutane, d-alanine, and d-homoalanine are important intermediates for the production of various active pharmaceutical ingredients and food additives. The preparation of these small chiral amine or amino acids with high water solubility still demands searching for efficient methods. In this work, we identified an ω-transaminase (ω-TA) from Sinirhodobacter hungdaonensis (ShdTA) that catalyzed the kinetic resolution of racemic 2-aminobutane at a concentration of 800 mM using pyruvate as the amino acceptor, leading to the simultaneous isolation of enantiopure (S)-2-aminobutane and d-alanine in 46% and 90% yield, respectively. In addition, (S)-2-aminobutane (98% ee) and d-homoalanine (99% ee) were isolated in 45% and 93% yield, respectively, in the kinetic resolution of racemic 2-aminobutane at a concentration of 400 mM coupled with deamination of l-threonine by threonine deaminase. We thus developed a biocatalytic process for the practical synthesis of these valuable small chiral amine and d-amino acids.
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Baogang Xie,Yalan Liu,Huiqin Zou,Yong Son,Huiyun Wang,Haipeng Wang,Jianghua Shao
d -Glucaric acid (GA) derivatives exhibit anti-cancerogenic properties in vivo in apples, but quantitative information about these derivatives is limited. Hydrophilic interaction-based HPLC with ultraviolet detection or mass spectrometry was developed to quantify GA and/or d -glucaro-1,4-lacton (1,4-GL) in apples. Although the formation of 1,4-GL from GA could be the prerequisite to exert biological effects in vivo , only a small portion of GA (<5%) was identified and converted to 1,4-GL in the rat stomach. The 1,4-GL content in apples ranged from 0.3 mg/g to 0.9 mg/g, and this amount can substantiate health claims associated with apples. The amount of 1,4-GL was 1.5 times higher in Gala and the ratio of 1,4-GL to GA was lower in Green Delicious apples than those in the other varieties. Our findings suggested that the variety and maturity of apples at harvest are factors that determine 1,4-GL content.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Gels
·
2024
·Vol. 10
·期 12
·808
·
IF 5
Feng Li,Ruoxi Yang,Jian Xu,Guohai Xu,Ye Wu
The concentration of small molecules reflects the normality of physiological processes in the human body, making the development of simple and efficient detection equipment essential. In this work, inspired by a facile strategy in point-of-care detection, two devices were fabricated to detect small molecules via photocurrent measurement. A linear response of the photocurrent against the concentration of the small molecules was found. In the first device, metal ions were introduced into gel substrates made by xanthan gum to enhance photocurrent response. N-phenyl-2-naphthylamine was measured when iron or manganese ions were used. L-Phenylalanine was measured when the gel was modified by samarium, iron, cerium, or ytterbium ions. L-(+)-Arabinose was detected via the gels modified by iron or holmium ions. D-(+)-Mannose was detected when the gel was modified by ytterbium, manganese, chromium, or sodium ions. L-Methionine was detected in the gels modified by samarium, zinc, or chromium ions. The second device was based on a paper sheet. A sugar-like molecule was first synthesized, which was then used to modify the paper. The detection was possible since the photocurrent showed a linear trend against the concentration of D-Trehalose. A linear fit was conducted to derive the sensitivity, whose value was found to be 5542.4. This work offers a novel, simple, and environmentally sustainable platform that is potentially useful in remote areas lacking medical devices.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Yun-Yun Quan,Tingting Pan,Zhongda Zhang,Shihua Wang,Guiyun Wang,Lichao Yu,Ye Wang,Xu-Feng Zang,Fangjun Zhang,Xiaoxia Ye,Xuebo Pan,Zu-Sheng Huang
Bacterial infections are closely correlated with the genesis and progression of cancer, and the elimination of cancer-related bacteria may improve the efficacy of cancer treatment. However, the combinatorial therapy that utilizes two or more chemodrugs will increase potential adverse effects. Image-guided photodynamic therapy is a highly precise and potential therapy to treat tumor and microbial infections. Herein, four donor–acceptor–π-bridge–acceptor (D–A–π–A) featured near-infrared (NIR) aggregation-induced emission luminogens (AIEgens) ( TQTPy , TPQTPy , TQTC , and TPQTC ) with type I and type II reaction oxygen species (ROS) generation capabilities are synthesized. Notably, TQTPy shows mitochondria targeted capacity, the best ROS production efficiency, long-term tumor retention capacity, and more importantly, the three-in-one fluorescence imaging guided therapy against both tumor and microbial infections. Both in vitro and in vivo results validate that TQTPy performs well in practical biomedical application in terms of NIR-fluorescence imaging-guided photodynamic cancer diagnosis and treatment. Moreover, the amphiphilic and positively charged TQTPy is able to specific and ultrafast discrimination and elimination of Gram-positive (G+) Staphylococcus aureus from Gram-negative (G−) Escherichia coli and normal cells. This investigation provides an instructive way for the construction of three-in-one treatment for image-guided photodynamic cancer therapy and bacteria elimination.
研究领域:
癌症研究
·
光动力疗法
·
材料科学
·
生物医学工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Yulin Fan,Fang Fu,Langqiu Chen,Jiping Li,Jing Zhang,Guochao Zhang,Jingyi Liao
Based on reconstructing conventional alkyl β - d -glucopyranosides, alkyltri(oxyethyl) β - d -glucopyranosides ( 4a ~ 4h , n = 6 ~ 16) were prepared by introducing a tri(oxyethyl) group ((–OCH 2 CH 2 –) 3 ) as a hydrophilic spacer to improve effectively the related water solubility. Their foaming performance, emulsifying property, hygroscopicity, solubility, and thermotropic phase transitions were further investigated. The experimental results showed that the phase transition temperature (T iso ) increased with increasing their alkyl chain length. Such β - d -glucopyranosides ( 4b ~ 4h , n = 7 ~ 16) showed a smectic focal conic-shaped texture. Their water solubility, surface tension, CMC value, and hygroscopicity decreased with increasing their alkyl chain length. Meanwhile, 4e (n = 10) had the best foamability. In the rapeseed oil/water system, 4f (n = 12) had excellent emulsifying property. Furthermore, 4 (n = 8, 10, 12) and SDS or CTAC had the synergistic effect because their experimental CMC value was smaller than their ideal CMC value (CMC id ).
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）