CARBON
·
2019
·
IF 7.466
Yi Yu,QingYi Zhu,Feifan Xiang,Yue Hu,Lijie Zhang,Xiangju Xu,Nannan Liu,Shaoming Huang
AuNPs/SWCNT, a hybrid nanomaterial which is single-walled carbon nanotube (SWCNT) modified with gold nanoparticles (AuNPs), has great application prospects for chemical and biological sensors. For the innate nanogap between tiny AuNPs deposited on sidewall of SWCNT, AuNPs/SWCNT is suitable for the fabrication of simple and effective electrical signal switching devices. Here, we develop a novel strategy of applying AuNPs/SWCNT to fabricate nanogap device for DNA hybridization detection. The detection strategy is based on the target DNA mediating the catalytic activity of the AuNPs by desorbing the probe DNA from AuNPs. The signal transduction depends on the growth of tiny AuNPs which is closely related to the AuNPs catalytic activity. Sensing occurs at the tiny AuNPs growing and connecting into a continuous nanowire along the SWCNT, leading to a conductimetric response. The biosensing device can detect target DNA with the detection limit of 1 pM and the ability to discriminate triple and quintuple base pair mismatches.
研究领域:
生物传感器
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2024
·
IF 1.2
Jiamei LI,An YAN,Mingquan WANG,Di LI
Targeted delivery of proteins into desired cell groups is crucial for disease therapy and cellular functionalization. However, current delivery methods face severe side effects and off-target problems, making it difficult to achieve cell-targeted protein delivery. Herein, we developed a user-friendly membrane fusion liposome for cancer cell-targeted protein delivery. Phosphorothioated DNA-mediated membrane fusion was employed as an efficient transmembrane delivery approach. The phosphorothioated DNA was capped with the AS1411 aptamer, which specifically recognizes nucleolar proteins on the cancer cell membrane, enabling a controllable delivery of proteins into targeted cancer cells. This delivery system exhibited commendable biocompatibility and targeted delivery ability, thereby realizing highly effective cancer cell inhibition in vitro. The in vivo results further suggested that the membrane protein-responsive membrane fusion delivery system offers a new avenue for highly biocompatible and targeted protein delivery.
研究领域:
生物化学
·
药物递送系统
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Wanhao Wu,Ying Wang,Yuxing Zhou,Yong Shao,Lihua Zhang,Hua Liu
DNA abasic site (apurinic/apyrimidinic (AP) site) has received much attention due to its involvement in diseases and development of selective genetic drugs. Fluorescence technique has been widely used in identifying the AP sequence environment with a fluorophore being the AP site-targeting probe. However, fluorescence enhancement for signal-on response with no need of labeling procedure still remains a challenge, especially for the AP site flanked by guanine context. Usually, the excited state of the AP site-targeting fluorophore is deactivated by electron transfer with the context guanine, the most oxidizable base in DNA. In this work, we found that 9-dicyanovinyljulolidine (DCVJ) can selectively target the apurinic site (namely, the orphan base is pyrimidine) and its fluorescence is significantly enhanced only when the flanking base is guanine. According to dependence of the fluorescence responses on base’ redox properties and base stacking-determined electronic properties, we proposed that the comparable electron energy levels of the DCVJ excited state and the flanking base are responsible for the observed fluorescence enhancement, although DCVJ has been reported as a molecular rotor-based probe. This turn-on fluorescence probe for the flanking guanine environment of the AP site exhibits a better selectivity in comparison with the previously used turn-off probe.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
MOLECULAR MEDICINE
·
2025
·
IF 6.4
Quanpeng Qiu,Yi Ding,Xiaolong Guo,Jing Han,Jiaqi Zhang,Yaping Liu,Junjun She,Yinnan Chen
Background Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice. Methods We conducted a Circle‐Seq analysis on clinical samples at different stages of colorectal cancer progression to examine the dynamic changes of eccDNA during the progression of colorectal cancer. We used breakpoint-specific PCR to verify candidate eccDNAs identified by Circle‐Seq. The results were further validated using the AOM/DSS-induced colorectal cancer model. Results There was an increase in the abundance of eccDNA with the progression of colorectal cancer. The genes associated with these eccDNA molecules were primarily related to signaling pathways involved in tumor development and metastasis. Our analysis also revealed that eccDNA abundance positively correlates with gene expression, and eccDNA derived from specific genes has potential value for the early diagnosis of tumors. Conclusions This study revealed a connection between eccDNA and colorectal cancer progression and highlights the clinical potential of eccDNA for the early diagnosis of colorectal cancer. Supplementary Information The online version contains supplementary material available at 10.1186/s10020-025-01164-y.
研究领域:
医学
·
分子生物学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 17
·9613-9619
·
IF 3.049
Jimin Yang,Lei Gao,Cheng Peng,Wei Zhang
In this work, a novel self-signal DNA electrochemical biosensor was constructed based on tungsten disulfide (WS2) nanosheets combined with poly(indole-6-carboxylic acid) (PIn6COOH) as the sensing interface. The WS2 nanosheets were synthesized via a simple solvent exfoliation method from bulk WS2, and then PIn6COOH was electropolymerized on the WS2 nanosheet-modified carbon paste electrode to obtain a unique nanocomposite. The electropolymerization efficiency was remarkably improved, ascribed to the physical adsorption between WS2 nanosheets and aromatic In6COOH monomers, resulting in an increase of the electrochemical response of PIn6COOH. Owing to the presence of π–π interactions between the conjugated PIn6COOH/WS2 nanocomposite and DNA bases, the probe ssDNA was noncovalently assembled on the nanocomposite substrate. After the hybridization of the probe ssDNA with the target DNA, the formation of the double-helix structure induced the resulting dsDNA to be released from the surface of the conjugated nanocomposite, accompanied with the self-signal regeneration of the nanocomposite (“signal-on”). The constructed PIn6COOH/WS2 nanocomposite was not only employed as an interface for DNA immobilization but also reflected the signal transduction stemming from DNA immobilization and hybridization without any external indicators or complex labeling processes. A detection limit of 2.3 × 10−18 mol L−1 has been estimated and a dynamic range of 1.0 × 10−17 mol L−1 to 1.0 × 10−11 mol L−1 has been shown for the detection of a PIK3CA gene related to lung cancer. Selectivity of the biosensor has been researched in the presence of noncomplementary and base mismatched DNA sequences.
研究领域:
生物传感技术
·
医学诊断
·
纳米材料科学
✓ 已验证
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yingyue Zhu,Yilin Cai,Yibo Zhu,Lixue Zheng,Jianying Ding,Ying Quan,Limei Wang,Bin Qi
The detection of ultralow concentrations of mercury is a currently significant challenge. Here, a novel strategy is proposed: the colorimetric detection of Hg 2+ based on the aggregation of gold nanoparticles (AuNPs) driven by a cationic polymer. In this three-component system, DNA combines electrostatically with phthalic diglycol diacrylate (PDDA) in a solution of AuNPs. In the presence of Hg 2+ , thymine (T)-Hg 2+ -T induced hairpin turns are formed in the DNA strands, which then do not interact with PDDA, enabling the freed PDDA to subsequently facilitate aggregation of the AuNPs. Thus, according to the change in color from wine-red to blue-purple upon AuNPs aggregation, a colorimetric sensor is established to detect Hg 2+ . Under optimal conditions, the color change is clearly seen with the naked eye. A linear range of 0.25–500 nM was obtained by absorption spectroscopy with a detection limit of approximately 0.15 nM. Additionally, the proposed method shows high selectivity toward Hg 2+ in the presence of other heavy metal ions . Real sample analysis was evaluated with the use of lake water and the results suggest good potential for practical application.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Xinglu Jiang,Kangjing Li,Beibei Xie,Jun Zhou
Taq DNA polymerase is the most widely used enzyme for PCR amplification and it contains 832 amino acids but no cysteines. Bovine serum albumin (BSA) is an 832-amino acid protein containing 35 cysteines, and at high temperature BSA can produce protein hydrogels through recombined disulfides and noncovalent bonds. When hydrogels are prepared using a mixture of Taq DNA polymerase and BSA, no disulfide bonds occur between them, and noncovalent bonds are the main forces holding together the Taq DNA polymerase with the hydrogels. During PCR amplification, temperature cycling can destroy the noncovalent bonds, thereby releasing Taq DNA polymerase from the hydrogels to synthesize DNA. The release rate, regulated through the variation of BSA concentration, allows for velocity-controlled amplification of DNA. As the amount of DNA products exceeds that of the Taq DNA polymerase released from hydrogels, a linear amplification replaces the exponential PCR amplification. When the amplification velocity begins to decrease, the time needed for reaching a plateau increase. This provides a broader detection window for the analysis of the end products, which correspond to the difference compared with the initial DNA concentration. The number of primers is continuously reduced with increasing cycles, which further causes a decline in the DNA amplification efficiency. The difference in the initial DNA template may cause a different consumption of primers, which can cause errors in the Ct values in the case of qPCR. Based on the slopes of the linear amplification curve, the Ct values can be calibrated to reduce this error, and a more accurate detection of the initial content of the target DNA can be implemented.
研究领域:
分子生物学
·
生物技术
·
生物化学
✓ 已验证
DOI
高影响因子
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
·
2015
·
IF 2.321
Jing-Jing Kong,Zeng-Ping Chen,Yao Chen,Xiu-Fang Yan,Ru-Qin Yu
Accurate quantification of genomic DNA is fundamental to many molecular analyses such as diagnostic assays and genotyping. Due to relatively low and varying extraction rates of DNA from cell lysates by genomic DNA extraction kits, it is desired to quantitatively determine DNA contents directly in cell lysates. However, the coexistence of scatterers (e.g., cell debris), absorbers, and intrinsic fluorophores in cell lysates prevents either UV–Vis spectrophotometric methods or fluorometric methods based on fluorescent dyes and ratiometric models to be directly applied to cell lysates. In this contribution, a novel fluorometric method based on an advanced chemometric model which can effectively eliminate the effects of scatterers and absorbers on fluorescence spectra of cell lysates was proposed for the direct quantification of DNA in lysates of CEM cells. Without cumbersome prior DNA extraction, the proposed method achieved quite stable and accurate DNA quantification with recovery rates in the range of 89%–115%, considerably better than that of either the fluorometric method based on the ratiometric model or the UV–Vis spectrophotometric method coupled with DNA extraction. The proposed method has the advantages of rapidness, simplicity, and high precision, and can be developed into a promising alternative for accurate quantification of genomic DNA.
研究领域:
分子生物学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Miao He,Yan Chen,Feifan Yin,Wenting Cheng,Zhongyun Wang,Yang Xiang
Type 2 diabetes is on the rise year by year, and 80 % of patients with type 2 diabetes die from cardiovascular complications. Strict control of blood glucose can significantly reduce the occurrence and development of diabetic microvascular complications, but can not effectively prevent the occurrence of diabetic macrovascular lesions. DNA methyltransferase 1 (DNMT1) plays an important role in developing phenotypic switching and secretion of inflammatory factors in vascular smooth muscle cells(VSMC), leading to the deregulation of vascular function. In this work, a biosensor based on methylation protection was designed. In the presence of the target DNMT 1, the formed methylation sites protect the dsDNA from being digested by the Hpa II restriction enzyme. At the same time, a large number of G-quadruplex are produced by the catalyzed hairpin assembly (CHA) reaction cycle, and the peroxidase-like activity of G-tetraplex and hemin is used to continuously catalyze the color change of substrate ABTS, thus realizing dual signal amplification. The binding of magnetic beads to dsDNA effectively reduces the background signal and enables a highly selective and sensitive analysis of the target DNMT 1 in complex biological samples, with a detection limit as low as 0.22 UmL −1 , showing a good linear relationship in the range of 0.22–10 UmL −1 . A new approach for the study of macrovascular damage is provided in type 2 diabetes.
研究领域:
医学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Science of Advanced Materials
·
2022
·Vol. 14
·期 5
·911-919
·
IF 1.067
Zhu Chunxia,Ming Shenqiu,Qiu Bin,Zhong Hui
FePt nanoparticles were in this study prepared at varied concentrations to assess their mechanism in regulating DNA injury and inactivation of Hela cells through mitogen-activated protein kinase (MAPK) signal pathway. The cells were divided into following 5 group sets: 1st, 2nd, 3rd,
4th and 5th, which were randomly intervened by following varied concentrations of FePt nanoparticles; 0, 5, 10, 15 and 20 μg/mL. Their action on cellular toxicity, proliferation and inactivation of cells was detected with CCK-8 method, while apoptotic condition was detected with
flow cytometry (FCM). The degree of DNA double chains fracture was detected with neutral single cell gel electrophoresis and immunofluorescence staining. The protein expressions of of EKR1/2, p-ERK1/2, p38 MAPK and p-p38 MAPK in every set were detected with Western Blot assay, while proliferative
activity in the 4th and 5th sets was lower than in the 1st set, and inhibiting action in the 4th set was best. There was no notable difference between 4th and 5th sets, and apoptotic rate in the 1st set was lower than in the other sets. The DNA percentage in the Hela cells was reduced and
Tial length was lengthened along with increased concentration of adopted FePt nanoparticles. The proliferation of Hela cells was restrained by FePt nanoparticles through restraining of the MAPK signal pathway, but growth of Hela cells was not affected, and DNA double-strand break (DSB) was
increased. The FePt nanoparticles could therefore be adopted as one kind of effective anti-tumor drug through increased DNA DSB in tumor cells based on killing effect of tumor cells and good compatibility for normal cells.
研究领域:
纳米技术
·
癌症生物学
·
细胞信号传导
·
毒理学
✓ 已验证
DOI
高影响因子
Matter
·
2025
·
IF 17.5
Yakun Gao,Biao Ma,Gangsheng Chen,Chengtao Xu,Ziyan Kong,Yanjie Chen,Chao Zhao,Duxin Chen,Wenwu Yu,Hong Liu
DNA data storage has emerged as a promising solution to address the challenges of data explosion due to its high storage density and durability. However, traditional storage architectures require substrate replacement and the use of environmentally unfriendly chemicals for multistep operations, hindering seamless and sustainable storage. Here, we report liquid metal (LM)-DNA, an integrated and sustainable DNA storage architecture that leverages a responsive liquid metal as a functionality-adaptive storage medium. LM-DNA allows cascaded storage operations within a single medium, including enzymatic DNA synthesis, hermetic encapsulation through thermal activation, and electrokinetic decapsulation driven by the electrocapillary effect. The green enzymatic DNA data writing and physical data encapsulation/decapsulation avoid toxic chemicals, ensuring a more sustainable route for DNA storage. Furthermore, the fluidity of liquid metal enables biomimetic operations such as file splitting, merging, and reshaping, offering unprecedented flexibility in information management. LM-DNA provides a promising foundation for advancing sustainable and biomimetic information storage technologies.
研究领域:
生物技术
·
材料科学
·
数据存储
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2015
·
IF 2.729
Shuang Ren,Huan Wang,Hongyi Zhang,Liqing Yu,Mingjie Li,Meng Li
Novel mesoporous carbon fibers (MCFs) were fabricated through a self-templating strategy and solution growth process. MCF modified glassy carbon electrode (MCF/GCE) was proposed for the direct electrocatalytic and simultaneous determination of four DNA bases (guanine (G), adenine (A), thymine (T) and cytosine (C)) using differential pulse voltammetry (DPV) measurement. The MCF/GCE exhibits satisfactory electrocatalytic activity by significantly enhancing the oxidation peak currents and lowering the oxidation potentials toward the four bases when compared with those on GCE. Especially, MCF greatly accelerates the electron transfer of pyrimidine base, which have high oxidation potentials and slow electron transfer kinetics. Moreover, MCF/GCE realizes the simultaneous determination of four DNA bases with low detection limits, high sensitivity and wide linear range. More important, the proposed electrode was successfully applied in the direct detection of bases in herring sperm DNA sample with satisfactory results. Besides, the MCF/GCE shows good long-term stability and reproducibility. These superior performances of MCF/GCE toward purine and pyrimidine DNA bases provide a good possibility for applying the MCF/GCE in our further DNA biosensor research.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）