Food Bioscience
·
2024
·
IF 4.8
Dami Li,Yaoyao Zhang,Xing Qiao,Hira Ijaz,Shangde Sun
Resveratrol (RES) is a natural non-flavonoid polyphenolic antioxidant. The low solubility of RES in water (26 mg/L) and oil (175.51 mg/kg) limited its antioxidant activity and bioavailability. In order to overcome these issues, in this work, a lipophilic resveratrol laurate was efficiently synthesized using RES as starting material in the presence of enzymes. The catalytic activities of enzymes (Lipase CALA, Lipozyme TL 100L, Lipase CALB, Novozym 435, Lipozyme TLIM, and Lipozyme RMIM) were investigated and screened. Moreover, reaction conditions were optimized using response surface methodology. Meanwhile, thermodynamic and kinetic analyses were also conducted to investigate the enzymatic mechanism. Results showed that, Lipozyme RMIM showed the best performance for resveratrol monolaurates regioselective synthesis. Two products, 3-resveratrol monolaurate (3-RC12) and 4′-RC12, were prepared and purified. The optimal reaction conditions for the preparation of 3-RC12, and 4′-RC12 were 1:65 substrate ratio and 11.7 wt% lipase dosage at 75 °C for 33.2 h. Under these conditions, the RES conversion reached a maximum of 98.04 ± 1.76%. The regioselective products were analyzed for antioxidant activity. Notably, the esterification site significantly impacts resveratrol monoesters' antioxidant activity. And 3-RC12 demonstrates superior ABTS and DPPH radical cation scavenging activity, surpassing RES and 4′-RC12. Notably, in yield and antioxidant activity, 3-RC12 is better than 4′-RC12, which make it a potential antioxidant for industrial application.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yanhua Li,Weijun Wang,Yangyong Deng,Junlong Gao,Jiayun Shi,Liqin Cai
The health benefits of fermented fruits have attracted consumers’ attention. High levels of antioxidant ability in the fermented kiwifruit extract were found at the early stage of fermentation. The co-fermention with Lactobacillus paracasei LG0260 and Kluyveromyces marxianus J2853 showed the highest ABTS radical scavenging ability (ABTS ⋅+ -SA) and superoxide dismutase (SOD) activity. Also, the typical antioxidant components of SOD activity, vitamin C concentration and total phenol content were highly correlated with ABTS ⋅+ -SA. Obviously, polyphenols in the fermented kiwifruit extract evolved into monophenols during fermentation. Compared to undigested samples, the activity of ABTS ⋅+ -SA and reducing power capacity (RP-CA) after the final intestinal digestion decreased and ranged 387.44–531.89 VCμg/mL, 650.95–981.63 VCμg/mL, respectively ( P < 0.05). Meanwhile, SOD activity on the 10th day of fermentation were still remained 222.82 U/mL, 206.98 U/mL and 217.23 U/mL, respectively. These results suggested that the fermented kiwifruit extract could exhibit antioxidant activity through tolerance to the digestive environment.
研究领域:
食品科学
·
微生物学
·
营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jianbin Deng,Shiqi Gao,Mengqi Liu,Weiquan Xie,Guang-Yu Pan
To better treat bacteria-infected wounds and promote healing, new wound dressings must be developed. In this study, we obtained PA@Fe by chelating iron trivalent ions (Fe 3+ ) with protocatechualdehyde (PA), which has a catechol structure. Subsequently, we reacted it with ethylene glycol chitosan (GC) via a Schiff base reaction and loaded vancomycin to obtain an antibacterial Gel@Van hydrogel with a photothermal response. The as-prepared Gel@Van hydrogel exhibited good injectability, self-healing, hemostasis, photothermal stability, biocompatibility, and antioxidant and antibacterial properties. Moreover, Gel@Van hydrogel achieved highly synergistic antibacterial efficacy through photothermal and antibiotic sterilization. In a mouse skin-damaged infection model, Gel@Van hydrogel had a strong ability to promote the healing of methicillin-resistant Staphylococcus aureus ( MRSA )-infected wounds, indicating the great potential application value of Gel@Van hydrogel in the field of treating and promoting the healing of infected wounds.
研究领域:
材料科学
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生物医学工程
·
伤口护理
·
药剂学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xiaoli Guo,Tingze Ren,Hongbo Li,Qingxin Lu,Xin Di
In this work, a natural deep eutectic solvent combined with microwave-assisted extraction (NADES-MAE) was studied and effectively utilized for the simultaneous extraction of the flavonoids from Scutellaria baicalensis. NADES composed of L-proline and urea were selected to achieve the efficient extraction of baicalin, baicalein, and wogonin. Box Behnken design (BBD) was applied to optimize the extraction conditions. The optimum conditions were as follows: L-proline and urea with a molar ratio of 2:1, water content of 40 %, extraction temperature of 110 °C, extraction time of 20 min, and liquid–solid ratio of 40 mL/g. Compared with the traditional extraction method, NADES-MAE displays the merits of higher yield, shorter extraction time, and less organic solvent consumption. In addition, the as-prepared NADES was characterized by FTIR and 1 H NMR, and the extraction mechanism for the flavonoids was investigated by density functional theory. The outcomes of biological activities in vitro showed that the extracts revealed desirable antioxidant activity. After assessing the ecological impact, the cytotoxicity of NADES was investigated and the results displayed that toxicity of the NADES was very low or even negligible with EC 50 > 2000 mg/L. The results indicated that the suggested approach was sensitive, green, and simple, showing good potential for analysis of active components from natural sources.
研究领域:
植物化学
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药理学
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分析化学
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 4
·619
·
IF 5.1
Kaijie Shang,Zhiheng Zhao,Hua Chen,Xiaonan Bian,Xianquan Zhong,Xiaoping Hu,Xue Lin,Lu Wang
The consumption of wampee has traditionally been utilized to alleviate gastrointestinal inflammation and associated disorders; however, its exact mechanism has remained unknown. The aim of this study was to elucidate the therapeutic efficacy and underlying mechanism of wampee polyphenol extract (WPE) in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). The findings revealed that WPE alleviated diverse symptoms of UC, regulated various inflammatory cytokines, and effectively protected the colon tissue structure and barrier integrity, thereby inhibiting LPS translocation. Moreover, WPE restored the richness and diversity of gut microbiota and optimized its structure at the phylum and genus levels, causing a notable improvement in short- chain fatty acid (SCFA) metabolism, particularly acetic acid, propionic acid, and butyric acid. Consequently, WPE was demonstrated to effectively suppress the LPS-induced TLR4-p38 MAPK/NF-κB signaling pathway by modulating gut microbiota and SCFA metabolism. These findings provided a theoretical basis for the use of wampee as a potential functional natural food for UC.
研究领域:
营养学
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胃肠病学
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免疫学
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微生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Quan Zhang,Bang-Xian Yu,Jun Long,Xue-Wu Chen,Fang Huang,Wen-Qi Wu,Yong-Da Liu,Guo-Hua Zeng,Ling-Hong Huang,Xin-Yuan Sun
Kidney stones, which have high prevalence and recurrence rates, often cause severe oxidative damage and inflammation. The ultrasmall hydrodynamic diameter of nanoparticles is crucial for their enrichment in the kidneys to exert biological activity. However, integrating crystallization inhibition and therapeutic functions into a single ultrasmall nanoparticle is challenging. A novel ultrasmall iron (Fe)–gallic acid (Ga) metal–phenolic networks (Fe–Ga MPNs) is developed for treating calcium oxalate (CaOx) nephropathies. These MPNs can specifically adsorb on the high-energy ( 1 ¯ 01 $\bar{1}01$ ) crystal face to inhibit the growth of CaOx monohydrate (COM), promoting the phase transition from highly toxic COM to low-risk CaOx dihydrate. Fe–Ga MPNs have broad-spectrum free radical scavenging abilities, reducing oxidative damage and inhibiting cell apoptosis. They exhibit sensitivity toward kidney damage, accumulating in injured renal tissue, reducing tubule injury and inflammation, improving tubule function, and inhibiting crystal formation. Fe–Ga MPNs also inhibit pro-inflammatory macrophage polarization and upregulate anti-inflammatory and highly phagocytic macrophage polarization. RNA sequencing analysis shows that Fe–Ga MPNs induce transcriptomic changes mainly involving immune regulation and citrate homeostasis pathways. In conclusion, the multifunctional nanonetwork Fe–Ga MPNs, with crystallization inhibition, antioxidant, and immune regulation properties, show great potential in treating CaOx nephropathies.
研究领域:
医学
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材料科学
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肾脏病学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
JinTao He,Qiong Peng,JingJing Wang,Jing Deng,Wen Li,QinLu Lin,FeiFei Zhong,Xu Xia
Improper storage methods cause food resources to be wasted, and the development of multifunctional intelligent packaging can realize freshness monitoring and extend the shelf life. In this study, an intelligent alizarin/thymol-loaded polycaprolactone/gelatin/zein nanofibrous film was prepared and achieved the dual functions of pH-responsive and antibacterial properties. The film was fabricated using electrospun technology and characterized by SEM, FT-IR, WCA, TGA, DSC, and mechanical property tests, which had good antioxidant properties (81.40 ± 0.74 % free radical scavenging). The film could effectively inhibit the growth of Escherichia coli , Staphylococcus aureus , and Shewanella putrefaciens within 12 h and exhibited light yellow-light purple-dark purple color change at pH 2–12, with good color stability and color responsiveness to ammonia in the environment. In the shrimp application, shelf life was extended by 2 days and freshness could be monitored by the color change. This scientific research opens up new possibilities for realizing multifunctional food packaging alternatives.
研究领域:
食品科学
·
材料科学
·
生物技术
·
包装工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Fanyi Guo,Jianfeng Li,Ziyu Chen,Tianxiao Wang,Ruyu Wang,Tianyao Wang,Yifeng Bian,Yifei Du,Hua Yuan,Yongchu Pan,Jianliang Jin,Huijun Jiang,Feng Han,Jiandong Jiang,Fan Wu,Yuli Wang
The excess production of reactive oxygen species (ROS) will delay tooth extraction socket (TES) healing. In this study, we developed an injectable thermosensitive hydrogel (NBP@BP@CS) used to treat TES healing. The hydrogel formulation incorporated black phosphorus (BP) nanoflakes, recognized for their accelerated alveolar bone regeneration and ROS-scavenging properties, and dl-3-n-butylphthalide (NBP), a vasodilator aimed at enhancing angiogenesis. In vivo investigations strongly demonstrated that NBP@BP@CS improved TES healing due to antioxidation and promotion of alveolar bone regeneration by BP nanoflakes. The sustained release of NBP from the hydrogel promoted neovascularization and vascular remodeling. Our results demonstrated that the designed thermosensitive hydrogel provided great opportunity not only for ROS elimination but also for the promotion of osteogenesis and angiogenesis, reflecting the “three birds with one stone” concept, and has tremendous potential for rapid TES healing.
研究领域:
口腔医学
·
生物材料学
·
组织工程学
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Zhicheng Tong,Yuanzhu Ma,Qiushi Liang,Tao Lei,Hongwei Wu,Xianzhu Zhang,Yishan Chen,Xihao Pan,Xiaozhao Wang,Huimin Li,Junxin Lin,Wei Wei,Chong Teng
Superficial cartilage defects represent the most prevalent type of cartilage injury encountered in clinical settings, posing significant treatment challenges. Here, we fabricated a cartilage extracellular matrix mimic hydrogel (GHC, consisting of Gelatin, Hyaluronic acid, and Chondroitin sulfate) to avoid the exacerbation of cartilage deterioration, which is often driven by the accumulation of reactive oxygen species (ROS) and a pro-inflammatory microenvironment. The GHC hydrogel exhibited multifunctional properties, including in situ formation, tissue adhesiveness, anti-ROS capabilities, and the promotion of chondrogenesis. The enhancement of tissue adhesion was achieved by chemically modifying hyaluronic acid and chondroitin sulfate with o-nitrobenzene, enabling a covalent connection to the cartilage surface upon light irradiation. In vitro characterization revealed that GHC hydrogel facilitated chondrocyte adhesion, migration, and differentiation into cartilage. Additionally, GHC hydrogels demonstrated the ability to scavenge ROS in vitro and inhibit the production of inflammatory factors by chondrocytes. In the animal model of superficial cartilage injury, the hydrogel effectively promoted cartilage ECM regeneration and facilitated the interface integration between the host tissue and the material. These findings suggest that the multifunctional GHC hydrogels hold considerable promise as a strategy for cartilage defect repair. Statement of Significance : Superficial cartilage defects represent the most prevalent type of cartilage injury encountered in the clinic. Previous cartilage tissue engineering materials are only suitable for full-thickness cartilage defects or osteochondral defects. Here, we developed a multifunctional GHC hydrogel composed of gelatin, hyaluronic acid, and chondroitin sulfate, which are natural cartilage extracellular matrix components. The drug-free and cell-free hydrogel not only avoids immune rejection and drug toxicity, but also shows good mechanical properties and biocompatibility. More importantly, the GHC hydrogel could adhere tightly to the superficial cartilage defects and promote cartilage regeneration while protecting against oxidation. This natural ingredients and multifunctional hydrogel is a potential material for repairing superficial cartilage defects.
研究领域:
组织工程
·
生物材料
·
骨科
·
生物医学工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Sun Yage,Zhao Xinrui,Zhang Qian,Yang Rong,Liu Wenguang
The hypoxia microenvironment post myocardial infarction (MI) critically disturbs cellular metabolism and inflammation response, leading to scarce bioenergy supplying, prolonged inflammatory phase and high risk of cardiac fibrosis during cardiac restoration. Herein, an injectable hydrogel is prepared by Schiff-base reaction between fructose-1,6-bisphosphate (FBP)-grafted carboxymethyl chitosan (CF) and oxidized dextran (OD), following by loading fucoidan-coated baicalin (BA)-encapsulated zein nanoparticles (BFZ NPs), in which immunoregulatory and metabolism improving functions are integrally included. The grafted FBP serves to enhance glycolysis and provide more bioenergy for cardiomyocytes survival under hypoxia microenvironment, and elevating cellular antioxidant capacity via pentose phosphate pathway. OD with intrinsic anti-inflammatory effect can induce M2 polarization of macrophages to accelerate inflammatory elimination. While facing the possibility of endothelial-to-mesenchymal transition (EndoMT) caused by excessive expressed TGF-β1 secreted from M2 macrophages, BFZ NPs can target endothelia cells and intracellularly release BA to regulate the level of fatty acid oxidation, resulting in retained endothelial features and decreased risk of cardiac fibrosis. After being injecting the hydrogel into rats’ infarcted cardiac, the 28 day-post surgical outcomes demonstrate its benign effects on restoring cardiac functions and attenuating adverse left ventricular remodeling. This study shows a promising measure for MI treatment with immunoregulating and metabolism regulation comprehensively.
研究领域:
生物医学工程
·
心脏病学
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Liling Wang,Yanbin Wang,Yuchuan Qin,Liang He
U.S. pecan nuts are rich in oil and phenolic compounds with important biological functions. At present, the reports about simultaneous extraction of oil and phenolic compounds are limited, and the traditional procedure is complicated and the yield is low. In this study, a new emulsion formed by hydrophobic deep eutectic solvent (DES) and water was used as extractant for synchronous extraction of oil and phenolic components from U.S. pecans, and subsequently the two were recovered from the upper and lower phases based on the incompatibility of DES and water, respectively. The oil and phenolic compounds obtained by the optimization of extraction conditions were 615.29 mg/g and 82.11 mg/g, respectively, which were higher than those obtained by conventional methods. At the same time, the extraction mechanism is discussed through the extraction kinetics and thermodynamics. The extraction process conformed to the Fick’s second law, and it was an endothermic process. After directional recovery by macroporous resin and back-extraction, the effectiveness of the method was proved by the analysis of fatty acid composition of oil, spectral characterization of phenolic compounds and antioxidant activities.
研究领域:
食品科学
·
植物化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Junjun Liu,Naibo Feng,Xinyu Jiang,Xirui Xin,Yubo Hou,Zhende Jiang,Laijin Lu,Weixian Yu,Bai Yang
Periodontitis is a common chronic inflammatory disease and the local accumulation of excessive reactive oxygen species (ROS) could cause oxidative damage to periodontal tissues. Thus, antioxidative therapy is considered to be the promising strategy for treating periodontitis. However, highly efficient antioxidant activity of biomaterials with low cost, ease of synthesis, and good biocompatibility are still highly desired. Here amylopectin from maize (AFM)-derived carbonized polymer dots (AFM-CPDs-240) with excellent antioxidant activity, good water solubility and biosafty is parepared. Detailed characterizations identify that the antioxidant properties and formation mechanism of AFM-CPDs, and antioxidant activity of AFM-CPDs-240 is much higher than Trolox (a hydrophilic derivative of vitamin E) due to the large contents of conjugated C=C and C=O. Besides, in vitro and in vivo experiments suggest AFM-CPDs-240 could effectively alleviate inflammatory response, inhibit alveolar bone resorption and promote bone formation, showing excellent therapeutic effects on periodontitis. Moreover, the label-free proteomics and western blot results demonstrate AFM-CPDs-240 could accelerate the recovery of periodontitis by blocking TLR4/NF-κB (Toll-like receptor 4/ nuclear factor-kappa B) signaling pathway. This study will develop a simple approach to design highly efficient antioxidant carbon dots with good biocompatibility, and also provide a new promising nanoplatform for treating diseases associated with ROS.
研究领域:
牙医学
·
材料科学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）