Molecular Catalysis
·
2025
·
IF 4.9
Hang Zhao,Xiuying Liu,Siyu He,Mingxia Guo,Yuhan Huang,Yifan Niu,Haiqing Jiang,Qian Li
Advanced oxidation processes (AOPs) are effective approaches for the removal of organic contaminants from water. In this study, a combined hydrogen peroxide-bicarbonate (H 2 O 2 -HCO 3 − ) oxidant system was engineered for a Fenton-like process catalyzed by lithium cobalt oxide (LCO). The oxidant system integrates hydrogen peroxide oxidation with bicarbonate's dual functionality as both an H 2 O 2 activator and pH buffer. Efficient contaminant degradation was achieved by the LCO/H 2 O 2 HCO 3 − system under neutral or weakly alkaline conditions, and the solution pH remained stable throughout the reaction, due to the presence of HCO 3 − . A comprehensive investigation into the optimization of reaction parameters governing the removal of a phenothiazine dye was conducted. The mechanism driving the generation of reactive oxygen species (ROS) which are responsible for the degradation of organic contaminants was elucidated. These ROS were identified as superoxide radicals, singlet oxygen, and hydroxyl radicals. The Co(III)/Co(II) redox pair in LCO served as the key active sites for activating the H 2 O 2 HCO 3 − . In addition, a possible pathway of the degradation of the phenothiazine structure was proposed based on ultraviolet-visible spectroscopy and liquid chromatography-mass spectrometry analysis. The LCO/H 2 O 2 -HCO 3 − system shows great promise as a sustainable Fenton-like approach for wastewater treatment, with cost-effectiveness, high efficiency, environmental friendliness, and compatibility with neutral or weakly alkaline conditions.
研究领域:
环境化学
·
材料科学
·
水处理技术
·
催化
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
NEUROCHEMICAL RESEARCH
·
2018
·Vol. 43
·期 7
·1439-1453
·
IF 2.772
Jia Jingjing,Zhang Ting,Chi Jieshan,Liu Xiaoma,Sun Jingjing,Xie Qizhi,Peng Sijia,Li Changyan,Yi Li
CeO 2 nanoparticles (nanoceria) have been used in many studies as a powerful free radical scavenger, and LXW7, a small-molecule peptide, can specifically target the integrin αvβ3, whose neuroprotective effects have also been demonstrated. The objective of this study is to observe the neuroprotective effect and potential mechanism of CeO 2 @PAA-LXW7, a new compound that couples CeO 2 @PAA (nanoceria modified with the functional group of polyacrylic acid) with LXW7 via a series of chemical reactions, in H 2 O 2 -induced NGF-differentiated PC12 cells. We examined the effects of LXW7, CeO 2 @PAA, and CeO 2 @PAA-LXW7 on the viability of primary hippocampal neurons and found that there was no significant difference under control conditions, but increased cellular viability was observed in the case of H 2 O 2 -induced injury. We used H 2 O 2 -induced NGF-differentiated PC12 cells as the classical injury model to investigate the neuroprotective effect of CeO 2 @PAA-LXW7. In this study, LXW7, CeO 2 @PAA, and CeO 2 @PAA-LXW7 inhibit H 2 O 2 -induced oxidative stress by reducing the production of reactive oxygen species (ROS) and regulating Bax/Bcl-2, cleaved caspase-3 and mitochondrial cytochrome C (cyto C) in the apoptotic signaling pathways. We found that the levels of phosphorylation of focal adhesion kinase (FAK) and of signal transducer and activator of transcription 3 (STAT3) increased significantly in H 2 O 2 -induced NGF-differentiated PC12 cells, whereas LXW7, CeO 2 @PAA, and CeO 2 @PAA-LXW7 suppressed the increase to different degrees. Among the abovementioned changes, the inhibitory effect of CeO 2 @PAA-LXW7 on H 2 O 2 -induced changes, including the increases in the levels of p-FAK and p-STAT3, is more obvious than that of LXW7 or CeO 2 @PAA alone. In summary, these results suggest that integrin signaling participates in the regulation of apoptosis via the regulation of ROS and of the apoptosis pathway in H 2 O 2 -induced NGF-differentiated PC12 cells. LXW7, CeO 2 @PAA, and CeO 2 @PAA-LXW7 can play neuroprotective roles by counteracting the oxidative stress and apoptosis induced by H 2 O 2 in NGF-differentiated PC12 cells. CeO 2 @PAA-LXW7 exerting a more powerful synergistic effect via the conjunction of LXW7 and CeO 2 @PAA.
研究领域:
神经科学
·
药理学
·
细胞生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today
·
2017
·
IF 21.695
Donghui Wang,Feng Peng,Jinhua Li,Yuqin Qiao,Qianwen Li,Xuanyong Liu
The selective tumor- and bacteria-killing property is promising for biomedical implants in directly contact with tumor tissue but still presents a considerable challenge. In this study, a butyrate-inserted Ni–Ti layered double hydroxide film (LDH/Butyrate) is prepared on the surface of nitinol alloy via a simple hydrothermal treatment. The prepared film can selectively inhibit tumor growth and metastasis and bacterial infection by taking advantage of the overproduced H 2 O 2 in tumor and infection microenvironments: the pro-tumor and pro-infection molecule H 2 O 2 can be consumed by LDH/Butyrate, and cytotoxic butyrate inserted in LDH/Butyrate is subsequently exchanged out. Such a novel endogenous stimuli-responsive platform is expected to prevent tumor overgrowth, metastasis and bacterial infection and will find a promising application in the design of localized drug-eluting systems.
研究领域:
生物医学工程
·
材料科学
·
癌症研究
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 50
·2106106
·
IF 18.808
Jie Shen,Huizhu Yu,Yimeng Shu,Ming Ma,Hangrong Chen
The efficacy of cancer therapy with reactive oxygen species (ROS) as the main therapeutic medium suffers from a deficiency of oxy-substrates, for example, insufficient endogenous hydrogen peroxide (H 2 O 2 ) in chemodynamic therapy (CDT) and inherent hypoxia in photodynamic therapy (PDT). Herein, a smart polyethylene glycol (PEG)-ylated nanosystem CaO 2 @ZIF-Fe/Ce6@PEG (abbreviation as CaZFCP) is constructed to achieve H 2 O 2 /O 2 self-supply and Ca 2+ overloading in tumor cells simultaneously for enhanced CDT/PDT. Under the weakly acidic tumor microenvironment, the activity components inside CaZFCP, that is, CaO 2 nanoparticles, Fe 2+ , and photosensitizer Chlorin e6 (Ce6) are released by the degradation of zeolitic imidazole framework-90 (ZIF-90). Thereinto, CaO 2 nanoparticles are further decomposed to generate H 2 O 2 and O 2 , which alleviates both the insufficient endogenous H 2 O 2 and hypoxia in tumor area, thus enhancing the efficiency of CDT and PDT by producing more hydroxyl radicals and singlet oxygen. Furthermore, Ca 2+ overloading induced by the decomposition of CaO 2 is available for amplifying intracellular oxidative stress, resulting in mitochondrial dysfunction, which further improves the efficacy of combined CDT/PDT. In vitro and in vivo experimental results confirm excellent tumor inhibition effect, which also provides a facile paradigm in ROS-involved cancer therapies.
研究领域:
癌症治疗
·
纳米技术
·
生物医学工程
·
药学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Chan Song,Wei Ding,Weiwen Zhao,Haibo Liu,Jie Wang,Yuewei Yao,Cheng Yao
In the last decades, enzyme mimics have been regarded as strong substitutes to natural enzymes . The construction of biosensors based on these enzyme mimics with competitive catalytic activity and substrate specificity has attracted a lot of research interest. Herein, for the first time, we investigated the capability of nanoscale FeS 2 to serve as enzyme mimics. Then, a facile and effective biosensor is fabricated based on its intrinsic peroxidase-like catalytic activity. In the presence of H 2 O 2 , FeS 2 nanoparticles (NPs) possess high peroxidase-like activity to 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation , which can be ascribed to the generation of hydroxyl radicals (·OH) from the H 2 O 2 decomposition catalyzed by FeS 2 NPs. As for TMB, the resulting Michaelis−Menten constant ( K m ) value of FeS 2 NPs is found to be about 12 times lower than that of natural horseradish peroxidase (HRP), highlighting the superiority of FeS 2 NPs. Based on these intriguing observations, a reliable colorimetric method is then developed for detection of H 2 O 2 and glutathione (GSH) by a simple mix-and-detect strategy. The detection limits of H 2 O 2 and GSH are as low as 0.91 μM and 0.15 μM (3σ/slope), respectively. Moreover, FeS 2 NPs can also catalyse the photoluminescence (PL) substrate terephthalic acid (TA) under the assistance of H 2 O 2 . This work remarkably extends the utilization of FeS 2 NPs in the construction of colorimetric and PL biosensors in the fields of biosensing, environmental monitoring, and medical diagnosis.
研究领域:
生物传感
·
环境监测
·
医学诊断
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2019
·Vol. 29
·期 3
·769-777
·
IF 1.913
Luo Kuan,Jiang Xinyu
Nitrogen-sulfur co-doped carbon quantum dots (N, S-CQDs) with good photoluminescence properties were prepared by hydrothermal method using citric acid (CA) and methionine (Met) as precursors. Co-doping with N and S facilitates the electron transfer rate and coordination interaction between N,S-CQDs and Fe(III) ions which acts as a quencher of fluorescence. Based on a simple redox principle, a highly sensitive and selective method for the detection of ascorbic acid (AA) and H 2 O 2 was successfully developed. The calibration curves obtained are linear for the current versus AA and H 2 O 2 concentration over the range 50–500 μM and 10–140 μM, respectively. And the detection limits for AA and H 2 O 2 are 4.2 μM and 1.9 μM, respectively. The quantitative analysis of AA and H 2 O 2 in various juices and H 2 O 2 disinfectant with Fe 3+ /CQDs or Fe 2+ /CQDs gave the recoveries of 87.8%–117.5% and 99.2%–106.4% with relative standard deviations ( RSD ) of 1.6–5.1% and 1.7–3.1%, respectively, showing satisfactory results for the determination of AA and H 2 O 2 in actual application. The proposed strategy may provide a new pathway to developing inexpensive and sensitive way for the detection of various redox reaction–involved system.
研究领域:
分析化学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 9
·1815-1823
·
IF 5.227
Yue Wang,Cheng Cheng,Ruofei Ma,Zhangrun Xu,Yukihiro Ozaki
Visualization of signaling molecules in single living cells is crucial for understanding cellular metabolism and physiology, which can provide valuable insights into early diagnoses and treatments of diseases. Highly sensitive in situ monitoring of intracellular analytes released from single living cells by virtue of label-free nanosensors is urgently needed, which can avoid interferences from molecular labeling. Here, we proposed an ultrasensitive strategy for in situ imaging of intracellular H2O2 in single living cancer cells by surface-enhanced Raman scattering (SERS) spectroscopy with the utilization of label-free Fe3O4@Ag core–satellite nanoparticles (NPs). The Fe3O4@Ag NPs can efficiently and selectively catalyze the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. Additionally, they exhibit excellent SERS activity that allows for in situ monitoring of intracellular H2O2 in living cells through establishing the correlation between the H2O2 level and the SERS intensity of the catalytic oxidation product of TMB. The H2O2 concentration is revealed through the SERS intensity of oxidized TMB with a good linear response in a wide range from 1 fM to 1 mM. Moreover, the intracellular H2O2 level in live cancer cells and imaging of the distribution of H2O2 inside single cells can be achieved by using such a label-free nanosensor based strategy. Our work demonstrates that the label-free Fe3O4@Ag NP-based SERS imaging and quantification strategy is a promising and powerful approach to assess intracellular H2O2 in living cells and allows us to monitor single-cell signaling molecules with nanoscale resolution.
研究领域:
纳米技术
·
生物医学工程
·
分析化学
·
细胞生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2022
·
IF 10.633
Ying Wang,Chuanjiang Jian,Yiqing Long,Xiaowen Xu,Yang Song,Zongning Yin
Pathological coagulation within an injured artery and the subsequent cardiovascular complications, such as stroke and heart attack, greatly threaten human life. Inspired by the biochemical features of acute arterial thrombosis, such as abundant activated platelets and hydrogen peroxide (H 2 O 2 ), we constructed platelet-targeted theranostic nanoparticles (CyBA/PFM NPs) with H 2 O 2 -triggered photoacoustic contrast enhancement and antithrombotic capabilities. CyBA/PFM NPs were designed to target platelet-rich clots via fucoidan segment within the carrier, which could be activated by H 2 O 2 to produce fluorescent “CyOH” molecules, thus turning on the photoacoustic signal. CyBA/PFM NPs showed obvious amplification of fluorescence following incubation with fresh clots, exhibiting efficient scavenging ability of intracellular reactive oxygen species (ROS). In a FeCl 3 -induced mouse model of carotid thrombosis, CyBA/PFM NPs significantly amplified the photoacoustic contrast in thrombogenic tissues, effectively eliminated ROS within the occlusion site, and suppressed the thrombus formation , accompanied by a normalization of the soluble CD40L level. Given their accurate imaging potential, potent antithrombotic activities and acceptable biosafety, CyBA/PFM NPs hold strong potential as nanoscale theranostics for H 2 O 2 -correlated cardiovascular diseases. Statement of significance In this study, we developed a platelet-targeted and H 2 O 2 -triggered nanosystem self-assembled from phenylboronated fucoidan/maltodextrin polymers and responsive near-infrared probes. The fucoidan segment within the carrier could facilitate the specific delivery of the therapeutic polymers and probes to the platelet-rich arterial thrombus. In a mouse model of FeCl 3 -induced arterial thrombosis, the system could be activated by H 2 O 2 to produce fluorescent “CyOH” molecules, thus turning on the photoacoustic signal and specifically imaging thrombosed tissues. Besides, CyBA/PFM NPs significantly effectively eliminated ROS within the occlusion site and suppressed the thrombus formation. Given their theranostic potential and acceptable biosafety, this system has great potential for H 2 O 2 -correlated cardiovascular diseases.
研究领域:
纳米技术
·
生物医学工程
·
心血管疾病
·
诊疗一体化
·
分子影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2018
·
IF 3.767
Fangyuan Xu,Wei Tang,Saisai Kang,Jinsheng Song,Xinrui Duan
Reactive oxygen species (ROS) are connected to aging and human diseases. The controlled generation of ROS, particularly H 2 O 2 , is necessary to maintain cellular fitness. Fluorescent probe based imaging is the most popular approach for H 2 O 2 detection. The photobleaching of the fluorophores is the significant limitation towards better signal-to-noise ratio and imaging resolution. We design and synthesize a highly sensitive and photo-stable fluorescent probe for detecting H 2 O 2 by using the 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) as an electron acceptor . After reacting with H 2 O 2 , the borate ester group in the probe was transformed into phenol group to form red emitted fluorophore that is highly photo-stable under the irradiation of UV light and excitation laser . A good linear relationship (R 2 = 0.9930) in the range of 1–20 μM of H 2 O 2 was obtained. The limit of detection (3σ/slope) of our probe was 61 nM, which make our probe one of the most sensitive fluorescent probes for H 2 O 2 detection. And the probe doesn't response to common other ROS species. The probe was successfully applied to endogenous intracellular H 2 O 2 imaging in live A549 lung cancer cells. More importantly, the imaging signal just had less than 10% difference in fluorescence intensity after 25 min continuous irradiation of excitation laser. Thus, we believe the proposed TCF-PB is very practical for sensing and imaging of trace amount of H 2 O 2 that endogenously generated in the live cancer cells, which is essential for the study of H 2 O 2 related cell signaling and human diseases.
研究领域:
生物化学
·
细胞生物学
·
癌症研究
·
分析化学
·
成像科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Mengmeng Sun,Qiushu Chen,Yingying Ren,Yong Zhuo,Shengyu Xu,Hanbing Rao,De Wu,Bin Feng,Yanying Wang
The effectiveness of chemokinetic therapy nanozymes is severely constrained because of the low H 2 O 2 levels in the tumor microenvironment. Unlike other self-produced H 2 O 2 nanozymes, the N -CNTs-encapsulated CoNi alloy (CoNiCoNC) with glucose oxidase and lactate oxidase activities has two ways to produce H 2 O 2 . It can facilitate the transformation of glucose and lactic acid into H 2 O 2 simultaneously. First, the H 2 O 2 generation pathway is favorable for aggravating energy metabolism. Second, some produced H 2 O 2 can be decomposed by CoNiCoNC to H 2 O and O 2 with the 4e − pathway to alleviate the TME hypoxia. Third, H 2 O 2 can be catalyzed to form OH to enhance reactive oxygen species (ROS) content. Through proteomic analysis, nanozymes substantially impact the metabolic pathways of cancer cells because of their aggravating energy metabolism. The high levels of ROS can cause mitochondrial lipid peroxidation and cellular ferroptosis. Consequently, the two-way H 2 O 2 -selective nanoenzymatic platform realizes the synergistic effect of starvation therapy and chemokinetics.
研究领域:
癌症治疗
·
纳米技术
·
生物化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-11
·
IF 3.8
Qiao Xilin,Cao Shuyu,Chen Shuaiyu,Guo Yan,Chen Nipi,Zheng Ying,Jin Bo
Oxidative stress induced endothelial dysfunction plays a particularly important role in promoting the development of cardiovascular diseases (CVDs). Salvianolic acid A (SalA) is a water-soluble component of traditional Chinese medicine Salvia miltiorrhiza Bunge with anti-oxidant potency. This study aims to explore the regulatory effect of SalA on oxidative injury using an in vitro model of H 2 O 2 -induced injury in human umbilical vein endothelial cells (HUVECs). In the study, we determined cell viability, the activities of Lactate dehydrogenase (LDH) and Superoxide dismutase (SOD), cell proliferation rate and intracellular reactive oxygen species (ROS). Flow cytometry was used to detect cell apoptosis. Western-blotting was used to evaluate the expression of cell senescence, apoptosis, autophagy and pyroptosis protein factors. The expression level of miRNA was determined by qRT-PCR. Compared with H 2 O 2 -induced HUVECs, SalA promoted cell viability and cell proliferation rate; decreased LDH and ROS levels; and increased SOD activity. SalA also significantly attenuated endothelial senescence, inhibited cell apoptosis, reversed the increase of LC3 II/I ratio and NLRP3 accumulation. Furthermore, miR-204-5p was regulated by SalA. Importantly, miR-204-5p inhibitor had similar effect to that of SalA on H 2 O 2 -induced HUVECs. Our results indicated that SalA could alleviate H 2 O 2 -induced oxidative injury by downregulating miR-204-5p in HUVECs.
研究领域:
药理学
·
心血管科学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EES Catalysis
·
2025
·
IF 8.1
Jie Yang,Lifang Chen,Xuya Zhu,Wenwen Shi,Mengxue Huang,Chang Liu,Ruimin Ding,Lin Gan,Xi Yin
H2O2 -related electrochemical reactions, including two-electron oxygen reduction reaction (2e-ORR), H2O2 oxidation reaction (HPOR), and H2O2 reduction reaction (HPRR), have received significant attention for the electrosynthesis H2O2 and energy storage. Understanding the complex structure-activity relationships among 2e-ORR/ HPOR/HPRR and their connections is crucial for further developing highly efficient catalysts and working systems. Herein, we unveil these intricacies by employing model Co-N-C catalysts with well-defined active site configuration (Co-N4-pyrrolic and Co-N4- pyridinic ) in a combined experimental and computational approach. We report the higher 2e-ORR/HPOR but lower HPRR activity of CoN4-pyrrolic site than the CoN4-pyridinic site based on their reaction free energy landscapes remodeled considering the chemisorption steps of O2 and H2O2 . The results reveal that the binding free energy of *OOH (ΔG*OOH ) can only be utilized as a reliable descriptor for 2e-ORR/HPOR activity, but not indicative of HPRR activity, regardless of the scaling relationship of the common reaction intermediates ( *OOH or *OH). The HPRR activity of CoN 4 sites strongly depends on the H2O2 adsorption strength and configuration. These findings provide valuable insights into the design of catalysts for H2O2 -related electrochemical energy conversion and storage systems.
研究领域:
电化学
·
催化
·
材料科学
·
能量转换与存储
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）