Frontiers in Immunology
·
2022
·
IF 8.786
Xin Zhao,Wenji Huang,Yanjie Shi,Jiahong Guo,Hehe Xiao,Ning Ji,Jianhua Feng,Huifeng Dang,Jun Zou
PLAAT1 is a member of the PLAAT protein family and plays important roles in tumor suppression, transglutaminase activation and peroxisomal biogenesis. Recently, PLAAT1 has been shown to promote degradation of p53 protein and cellular organelles such as mitochondria, endoplasmic reticulum and lysosome. In this study, we show that PLAAT1 inhibits the production of type I interferon and promotes virus replication in zebrafish. Overexpression of Plaat1 in zebrafish cells suppresses antiviral responses and promotes virus replication. Mechanistically, PLAAT1 interacts with IRF3 and IRF7 to initiate degradation of IRF3 and IRF7, which can be attenuated by 3-methyladenine, an inhibitor of autophagosome. Our study provides novel insights into the functions of PLAAT1 in host immune response to viral infection.
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 33
·16177-16182
·
IF 3.925
Shuyan Xiao,Liang Sun,Jiping Lu,Zhongping Dong
A label-free fluorescent biosensor based on the clenbuterol binding aptamer and the fluorescent intercalator SYBR Green I (SGI) was established for the sensitive and selective detection of clenbuterol. In the absence of clenbuterol, a weak fluorescence signal of SGI was observed. In the presence of clenbuterol, the specific recognition and high-affinity binding of the aptamer with clenbuterol induced a conformational change of the aptamer, and a longer double-stranded DNA was formed. As a result, an enhancement in the fluorescence of SGI could be found. Under optimal conditions, the fluorescence change ratio increased linearly with clenbuterol concentration from 50 nM to 450 nM, with a detection limit of 18.8 nM. In addition, the proposed aptasensor showed good selectivity and was successfully applied for clenbuterol quantification in pork samples with recoveries of 90.37–104.98%.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2018
·Vol. 32
·期 4
·e4231
·
IF 3.581
Xue-Wen Liu,Yang Xiao,Wei-Gen Peng,Li-Jin Zhao,You-Ming Shen,Song-Bai Zhang,Ji-Lin Lu
Two ruthenium complexes containing a new ligand phipz (phipz = 2-(1,10-phenanthroline)-1H-imidazo[4,5-b]phenazine) were designed and synthesized. These complexes were found to inhibit the DNA supercoiled relaxation mediated by topoisomerase I (topo I), cleave DNA under irradiation and bind to calf thymus DNA through intercalative mode. Furthermore, complex 2 shows higher photocleavage activity, topo I inhibition activity and DNA affinity than complex 1 . Additionally, introduction of phenazine unit may be the reason that two complexes exhibit DNA ‘light switch’ behavior. The present work shows that two complexes might be potential as new DNA ‘light switches’, DNA photocleavers and topo I inhibitors.
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Acta Biomaterialia
·
2021
·
IF 8.947
Jing Cao,Yu Sun,Cong Zhang,Xue Wang,Yiqing Zeng,Tao Zhang,Pintong Huang
Sonodynamic therapy (SDT) represents a viable approach to overcoming the limited ability of photodynamic therapy to penetrate biological barriers. However, pancreatic tumors contain a hypoxic microenvironment that limits the efficacy of oxygen-dependent type II SDT, complicating efforts to develop reliable, stable, and hypoxia-tolerant sonosensitizer. Herein, a tablet-like TiO 2 /C nanocomposite with a metal-organic-framework (MOF)-derived carbon structure was designed and found to be hypoxia-tolerant and stable in response to repeated ultrasound irradiation, enabling the TiO 2 /C-mediated generation of large quantities of reactive oxygen species (ROS) and thereby achieving efficacious type I SDT. Importantly, this nanocomposite continued to generate ROS in response to repeated ultrasound irradiation, and was able to induce tumor cell apoptosis via SDT-induced DNA damage in vitro and in vivo . This TiO 2 /C nanocomposite also exhibited good biocompatibility and did not induce any apparent toxicity in vitro and in vivo . Together, these data highlight TiO 2 /C as a valuable nanocomposite capable of facilitating repeated type I SDT, making it a promising tool for the treatment of hypoxic solid pancreatic tumors. Statement of significance In this research, a tablet-like TiO 2 /C nanocomposite with a metal-organic-framework (MOF)-derived carbon structure was designed, which exhibited great stability upon repeated ultrasound irradiation, hypoxic-tolerant ability and good biocompatibility. After ultrasound irradiation, TiO 2 /C could efficiently generate reactive oxygen species in an oxygen-independent manner, which overcame the limitation of pure TiO 2 nanoparticles . Therefore, it was applied to repeated type I sonodynamic therapy of hypoxic pancreatic tumor.
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高影响因子
Journal of Radiation Research and Applied Sciences
·
2020
·
IF 1.804
Xue-Wen Liu,Ning-Yi Liu,Yuan-Qing Deng,Shan Wang,Ting Liu,Yu-Cai Tang,Yuan-Dao Chen,Ji-Lin Lu
ABSTRACT In recent years, ruthenium (II) complexes have attracted attention due to high DNA topoisomerase I inhibitory activities and good anticancer activities. Herein, an asymmetric ligand 2-(5-(1, 10-phenanthroline))-1 H-4,5-diphenyl-imidazole (pdpi) and its ruthenium complexes were synthesized. DNA-binding mode of two complexes was determined as intercalation by using UV–vis spectra, emission spectra, viscosity, and molecular docking experiments. DNA photocleavage experimental results show that two ruthenium complexes effectively cleave plasmid DNA by producing singlet oxygen. Furthermore, they both displayed good topo I inhibition activities. The cytotoxic activities results show that complex 2 displays better antitumor activities against Eca-109 cells and A549 cells (IC 50 = 25.97 ± 3.33 μM and 28.83 ± 2.49 μM, respectively) compared to complex 1 .
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Laser & Photonics Reviews
·
2025
·
IF 10
Ziyi Niu,Pengfei Zhang,Wenbo Chen,Junyang Xia,Zhili Zhang,Jinwei Liu,Yanyan Li,Bitao Liu,Lei Zhao
Near-infrared (NIR) mechanoluminescence (ML) holds significant promise for in vivo biomechanical detection and imaging due to its self-powered excitation and self-recovery capabilities. However, its broader application remains constrained by the limited availability of suitable ML materials and the narrow spectral range of NIR emission. In this study, we report the Y 3 Ga 5 O 12 (YGO): Cr 3+ , Nd 3+ , Yb 3+ /polydimethylsiloxane (PDMS) NIR-ML composite with broadband NIR emission tunable from 600 to 1100 nm, covering both the NIR-I and NIR-II windows. The ML of composite device is driven by an interfacial triboelectric effect arising from the mechanical interaction between the inorganic particles and the polymer matrix. Comparative studies on photon penetration through biological tissues reveal that co-doping with Nd 3+ and Yb 3+ markedly enhances tissue penetration depth. Specifically, the luminescence decay rate in biological tissues is reduced by 20.8% relative to Cr 3+ single doping, indicating improved optical persistence and signal transmission. These findings demonstrate the potential of full-spectrum NIR-ML devices for high-resolution, deep-tissue biomechanical sensing and imaging. This work provides a generalizable design strategy for the development of broadband NIR-ML materials tailored for in vivo mechanical diagnostics.
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产品关联
DOI
高影响因子
DRUG DELIVERY
·
2025
·
IF 8.1
Mengjie Rui,Lei Fang,Mengfei Jia,Wenyan Liang,Yinfeng Li,Yaoshun Ruan,Chunlai Feng
Apolipoprotein A-I (ApoA-I) mimetic peptides have garnered attention as potential anticancer agents owing to their role in cholesterol metabolism and ability to interact with the SR-BI receptor. However, their tendency to form lipid-bound structures in circulation limits their tumor-targeting therapeutic potential and raises the risk of off-target effects. In this study, we engineered a stimuli-responsive ApoA-I mimetic peptide by incorporating reactive oxygen species (ROS) -responsive amino acid derivatives into its sequence. Under normal physiological conditions, the peptide adopted a disordered conformation, minimizing nonspecific interactions. In contrast, the exposure to the tumor microenvironment, which is characterized by low pH and elevated ROS, could trigger a conformational transition to a structured α-helical state, thereby enhancing its membrane-disruptive and tumor-targeting capabilities. Molecular dynamics simulations predicted a rapid increase in α-helical content for the peptide candidate 5A under tumor-like conditions. These predictions were experimentally validated using circular dichroism spectroscopy, liposome leakage assays, and transmission electron microscopy, which demonstrated that peptide 5A effectively interacted with lipid membranes only upon activation in a tumor-like environment. In vitro cytotoxicity assays further confirmed the selective anticancer activity of peptide 5A under acidic conditions, while in vivo imaging and tumor inhibition studies in breast cancer models revealed significant tumor accumulation and a tumor growth inhibition rate of up to 71.43% at a 6 mg/kg dose. Our results demonstrated the potential of stimuli-responsive ApoA-I mimetic peptides for targeted cancer therapy, offering a promising strategy to enhance therapeutic efficacy while minimizing systemic toxicity.
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Jiajia Wang,Li Li,Ruyi Wei,Renfeng Dong
Here, we report the first PbS quantum dot (QD)-based micromotors with NIR-I light-driven photocatalytic propulsion and NIR-II fluorescence. Under the irradiation of NIR-I light (808 nm), PbS QD-doped cuprous oxide (Cu2O@PbS) micromotors can display efficient propulsion in a variety of biocompatible fuels such as malic acid, glucose, and urea. Among them, the Cu2O@PbS micromotors exhibit the best propulsion performance in a very low concentration of malic acid, with an average speed as high as 11.86 μm/s. The enhanced NIR-I photocatalytic activity of Cu2O@PbS micromotors benefits from the doping of NIR-I PbS QDs that can be excited by NIR-I light and exhibit high electron transport efficiency. The doped PbS QDs can effectively increase the absorption efficiency of the micromotors in the NIR-I region while also inhibiting the recombination of photogenerated electron–hole pairs. Interestingly, due to the presence of NIR PbS QDs, the Cu2O@PbS micromotors demonstrate prominent and stable NIR-II fluorescence (emission wavelength: 1100 nm), which offer promising potential for visualization of their position in vivo. In comparison to other photocatalytic micromotors, the simple fabrication strategy, excellent NIR-II fluorescence, together with the NIR-I light-dependent propulsion behavior of the current Cu2O@PbS micromotors, thus pave the way for further development of advanced smart “robots” for intelligent biomedical applications.
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高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jun Li,Yao-Yao Fan,Jie Wen,Jing Zhang,Zhi-Qi Zhang
The HIV-Ι trans-activation responsive (TAR) RNA–trans-activator of transcription (Tat) protein complex is crucial for the efficient transcription of the integrated human immunodeficiency virus-I genome and is an established therapeutic target for AIDS diagnosis and treatment. Developing a sensitive strategy for the TAR RNA-binding ligand assay could provide antiviral leads with a radically new mechanism for the treatment of AIDS. Herein, a new TAR RNA-binding ligand assay platform was established using a signal amplification strategy that combines aggregation-induced emission (AIE) with a metal-enhanced fluorescence (MEF) concept. The tetraphenylethylene (TPE) derivative was labeled on the Tat peptide as a fluorescent molecule, while the TAR RNA was immobilized on the surface of the Fe3O4@Au@Ag@SiO2 nanoparticles (NPs) to specifically bind the TPE-Tat peptide. The TPE-Tat peptide was weakly emissive itself while emitting strongly in the NP–TAR–TPE-Tat complex by the AIE and MEF signal amplification effect. It was confirmed by known Tat peptide competitors that this system could be applied to the screening and detection of TAR RNA-binding ligands because they could replace the TPE-Tat peptide from the complex and make the system fluorescence decrease. When this system was adopted to test four candidate ligands, it was found that bisantrene had a favorable TAR RNA-binding ability. The proposed AIE–MEF strategy not only provides a sensitive and reliable method for the TAR RNA-binding ligand assay but also can avoid the influence of ligands on fluorescent detection in the conventional displacement assay.
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高影响因子
ANALYTICAL LETTERS
·
2020
·
IF 1.467
Wanxin Dong,Xinxin Mo,Yu Wang,Qin Lei,He Li
In this work, a novel flexible photoelectrochemical immunosensor is developed to determine cardiac troponin I (cTnI) in serum. The flower-like ZnIn2S4 nanospheres (ZIS), as signal amplification material, were successfully synthesized by a solvothermal method. The ZIS and Bi2Se3 were modified on the indium tin oxide- polyethylene terephthalate (ITO-PET) electrode, which effectively accelerates the electronic transition and improved the photocurrent conversion efficiency (signal on). However, the binding of antigen–antibody proteins blocks the electron transfer, resulting in a greatly reduced photocurrent (signal off). By adding the second antibody modified by CdSe quantum dots (CdSe QDs-Ab2), the photocurrent is increased (signal on). The constructed immunosensor has a linear range from 0.08 to 40 ng/mL with a detection limit of 0.026 ng/mL, which shows excellent selectivity, high sensitivity, and good stability. The photoelectrochemical immunosensor has broad application prospects for the clinical examination of myocardial infarction.
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Inorganic Chemistry Frontiers
·
2023
·Vol. 11
·期 1
·156-163
·
IF 7
Jiajing Wu,Jing-Li Qi,Yue Guo,Shufang Yan,Wenlong Liu,Sheng-Ping Guo
Hybrid copper halides have been rapidly developed because of their high photoluminescence quantum yield (PLQY), good water resistance, and variable structures; nevertheless, the controlled synthesis and interconversion of multiple hybrid copper halides containing different coordination geometries are rarely reported. In this study, using ethyltriphenylphosphonium as the templating cation [EtPh3P]+, three stable hybrid copper bromides with diverse inorganic anion skeletons, (EtPh3P)CuBr2 (112), (EtPh3P)2Cu2Br4 (224), and (EtPh3P)2Cu4Br6 (246), were synthesized by carefully tuning the feeding ratio of CuBr and (EtPh3P)Br. The emission peaks were located at 536, 546, and 580 nm with optical absorption edges of 3.64, 2.91, and 2.82 eV, respectively, showing a gradual redshift as the inorganic units change from a monomer (112) to dimer (224) and then to a tetramer (246). More interestingly, multiple solvents, including methanol (MeOH), ethanol (EtOH), isopropanol (IPA), acetic acid, ethylene glycol, and water with heat, can induce the spontaneous conversion of 112 and 224 to 246, which is attributed to the affinity of the organic salt with the solvent. Additionally, the crystal structure can transform from 246 into 112 (224) or can undergo a reversible dynamic conversion between 112 and 224 using the crystallization method. This work not only provides a strategy to adjust the inorganic units of the crystals but also to achieve multi-state structural transitions, making them viable candidates for next-generation multifunctional optoelectronic devices.
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CHEMISTRY OF MATERIALS
·
2024
·
IF 7.2
Ranran Gu,Kai Han,Jiance Jin,Hao Zhang,Zhiguo Xia
Processing luminescent metal halide microcrystals into counterpart nanocrystals (NCs) can rationally enhance the resolution in X-ray scintillation imaging. It remains a challenge for the controlled synthesis of the hybrid NC luminescence materials, which is important for the fabrication of the organic films. Herein, we design the synthesis of copper(I)-based halide Cu6I8(bu-ted)2 (Cu6I8C20H42N4) (bu-ted: 1-butyl-1,4-diazabicyclo [2.2.2] octan-1-ium) NCs via a surfactant-assisted method utilizing surface tension to limit the crystal size. Cu6I8(bu-ted)2 NCs with prominent near-to-unity photoluminescence quantum yield results in ultrahigh light output that is calculated to be 480% of a commercial Lu3Al5O12:Ce3+ scintillator with a low detection limit of 32 nGy/s. X-ray imaging with a spatial resolution of 17 lp mm–1 is demonstrated based on the fabrication of large-area Cu6I8(bu-ted)2 composite scintillation screens. This study provides a foundation method to nanocrystallize the copper(I)-based hybrid halide scintillators for prominent X-ray imaging.
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