JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jie Zhao,Gai Gao,Jing Ding,Wei Liu,Tao Wang,Liang Zhao,Jiangyan Xu,Zhenqiang Zhang,Xiaowei Zhang,Zhishen Xie
The simultaneous enhancement of lipophagy and mitochondrial biogenesis has emerged as a promising strategy for lipid lowering. The transcription factor EB (TFEB) exhibits a dual role, whereby it facilitates the degradation of lipid droplets (LDs) through the process of lipophagy while simultaneously stimulating mitochondrial biogenesis to support the utilization of lipophagy products. The purpose of this study was to explore the effect of astragaloside I (AS I) on hyperlipidemia and elucidate its underlying mechanism. AS I improved serum total cholesterol and triglyceride levels and reduced hepatic steatosis and lipid accumulation in db/db mice. AS I enhanced the fluorescence colocalization of LDs and autophagosomes and promoted the proteins and genes related to the autolysosome. Moreover, AS I increased the expression of mitochondrial biogenesis-related proteins and genes, indicating that AS I promoted lipophagy and mitochondrial biogenesis. Mechanistically, AS I inhibits the protein level of p-TFEB (ser211) expression and promotes TFEB nuclear translocation. The activation of TFEB by AS I was impeded upon the introduction of the mammalian target of rapamycin (mTOR) agonist MHY1485. The inhibition of p-mTOR by AS I and the activation of TFEB were no longer observed after administration of the Akt agonist SC-79, which indicated that AS I activated TFEB to promote lipophagy-dependent on the Akt/mTOR pathway and may be a potentially effective pharmaceutical and food additive for the treatment of hyperlipidemia.
研究领域:
药理学
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代谢学
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分子生物学
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细胞生物学
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内分泌学
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PubMed
高影响因子
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
·
2026
·
IF 4.5
Jiang F,Ma Y,Zhang Y,Shang D,Dong W.
Tryptophan (Trp)-substituted antimicrobial peptides (AMPs) exhibit enhanced interactions with bacterial cell membranes, potentially improving their antimicrobial efficacy. Klebsiella pneumoniae (20.59% of 2054 ICU isolates) is resistant to multiple clinically used antibiotics and presents significant treatment challenges. In the present study, three Trp-modified peptides (I4W, L12W, and I1WL5W) are generated by substituting Ile or Leu residues in temporin-1CEb, a peptide derived from frog skin, with Trp at various sites to assess their antibacterial effects and mechanisms against K . pneumoniae . Compared with L12W, both I4W and I1WL5W display superior antimicrobial activity and lower cytotoxicity. Mechanistic studies reveal that AMPs exert antibacterial and bactericidal effects through bacterial surface charge neutralization, insertion into bacterial cell membranes, increase permeability of both the inner and outer membranes, and disruption of membrane integrity. Notably, I1WL5W exhibit the most potent membrane-disrupting activity. Assessment of the impact of Trp-containing peptides on bacterial biofilms reveals that these peptides not only inhibit exopolysaccharide production and biofilm formation but also degrade preformed biofilms. A murine lung infection model is established to investigate the therapeutic efficacy of I1WL5W against MDRKP 1203-induced lung infection in mice. Compared with the control treatment, treatment with I1WL5W results in reduced bacterial counts and levels of IL-6 and TNF-α in both the blood and lung tissues of MDRKP 1203-infected mice, and treatment with I1WL5W improves lung tissue structure. The present study provides valuable insights for designing Trp-containing peptides with potent antimicrobial properties by facilitating their penetration across bacterial membranes.
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高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2026
·
IF 2.4
Linxue Yan,Tianyi Wang,Kangrui Sun,Kangnan Cheng,Jiantuan Jia,Yafei Guo,Tianlong Deng
Abstract The isothermal dissolution equilibrium method was employed to investigate the phase equilibrium and phase diagram of the ternary system (Na+, Cs+//Cl––H2O) at 313.15 K. This ternary system features the coexistence of double salts and solid solutions. The solid solution, formulated as ([Cs1–x(Na·H2O)x]Cl), was characterized by wet-residue analysis, X-ray diffraction, scanning electron microscopy, energy-dispersive spectroscopy, and thermogravimetry–differential scanning calorimetry (TG–DSC). Three representative equilibrium compositions of the solid solution and their respective proportions within the crystallization region were determined, and the results were compared with those of the two pure salts (NaCl and CsCl). The physicochemical property diagrams indicate that both density and refractive index increase with rising cesium chloride content. A comparative phase diagram of the ternary system (Na+, Cs+//Cl––H2O) was constructed over the temperature range from 298.15 to 318.15 K. The results show that as temperature increases, the crystallization region of NaCl expands, whereas the crystallization regions of CsCl·2NaCl·2H2O and [Cs1–x(Na·H2O)x]Cl simultaneously contract.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-11
·
IF 6.408
Wang Yue,Zhai Hongguo,Yin Jiaqi,Guo Qi,Zhang Yuhao,Yang Qingqing,Li Falan,Sun Xia,Guo Yemin,Zhang Yanyan
With the development of exonuclease, the exonuclease has been used to construct a variety of aptasensor and to realize the signal amplification. Among them, based on silver nanoparticles (Ag NPs) and exonuclease I (Exo I)-assisted cycle signal amplification strategy, we designed a novel high-sensitivity dual-target electrochemical biosensor to detect Pb 2+ or Hg 2+ in water. In the presence of Hg 2+ , the Hg 2+ was fixed to the aptamer chain by thymine-Hg 2+ -thymine (T-Hg 2+ -T), resulting in the decrease of signal. When Pb 2+ was present, DNA single strand S2 dissociated and was bound to Pb 2+ , which automatically triggered Exo I to selectively cut the single chain from the recognition site to achieve the cyclic amplification of the electrochemical signal. The interaction between aptamer and Exo I was investigated by gel electrophoresis. Under the optimum conditions in the scan range -0.20 to 0.60 V, the biosensor had high sensitivity with a linear range of 100 pg/L to 10.0 mg/L, Pb 2+ or Hg 2+ , and the detection limits were 17.0 pg/L ( R 2 = 0.993) and 12.0 pg/L ( R 2 = 0.993), respectively. The relative standard deviation (RSD) of the sensor was 0.5–2.6%, and the recovery of spiked standard solutions was between 98.3 and 110%. The cycle amplification strategy supported by this enzyme has promising applications in detection of the two metal ions in various fields. Graphical Abstract
研究领域:
分析化学
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环境科学
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生物化学
·
纳米技术
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PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 134
·期 46
·45484
·
IF 1.86
Teng Xue,Erjun Tang,Jian Zhou,Ruitao Han,Shaojie Liu
A coordinating ionic liquid (IL), 1-[(diethyl amine)amine]ethyl-3-methyl imidazolium chloride ([N 3 MIM]Cl), was prepared as an alternative to a simple organic ligand to coordinate to copper(I) bromide (CuBr). We, thereby, obtained a novel catalyst for atom transfer radical polymerization (ATRP) reactions. This catalyst was applied to the ATRP of methyl methacrylate in the IL 1-allyl-3-methyl imidazolium chloride ([AMIM]Cl). The chemical structures of the ILs obtained were confirmed by Fourier transform infrared spectroscopy, mass spectrometry, and 1 H-NMR analyses. The coordination ability of [N 3 MIM]Cl was assessed by cyclic voltammetry, and the redox potential of [N 3 MIM]Cl–CuBr was −0.507 V. The [N 3 MIM]Cl–CuBr complex was expected to be a markedly more active catalyst than the amine DETA–CuBr complex. The coordination mode toward CuBr was also examined. The [N 3 MIM]Cl–CuBr catalyst system showed good controllability in the aforementioned ATRP reaction in [AMIM]Cl. The Cu catalyst was easily separated from the obtained polymer with the coordinating IL as a ligand. Consequently, the coordinating IL overcame the shortcomings of traditional organic ligands, such as poor compatibility with IL media and poor separation of the catalyst from the polymer; this makes it highly promising for applications in the ATRP field. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45484.
研究领域:
高分子化学
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催化化学
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功能材料
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绿色溶剂
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配位化学
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电化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jinyan Gu,Lianzhu Lin,Mouming Zhao
Due to the difficulty in obtaining highly branched rhamnogalacturonan-I (RG-I) type pectin, the relationship between the extent of RG-I branching (EB) of pectin and prebiotic/immunomodulatory activity has not been systematically investigated. Moreover, it is only possible to establish a structure–activity relationship using pectin that is highly purified and accurately characterized. In this study, a homogeneous highly branched RG-I type pectin (LBP-P4, a final product) with dual proliferative effects on Bifidobacterium and macrophage was effectively purified for the first time using enzyme hydrolysis combined with ultrafiltration. The RG-I content and EB of LBP-P4 reached 97.32 and 77.12, respectively. Its two branches were composed of arabinan and arabinogalactan-II, containing → 5)-Araf-(1→, →3)-Araf-(1→, →3,6)-Galp-(1→ and →6)-Galp-(1→ residues). The structure–activity relationship analysis indicated that strong prebiotic/immunomodulatory activity of LBP-P4 was depended on its high EB, which was further confirmed by the molecular docking simulation between low/high branched pectin with β-1,6-galactosidase, α-l-arabinanase, and Toll-like receptor 4 (TLR4).
研究领域:
食品科学
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生物化学
·
免疫学
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微生物学
·
药学
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PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.6
Kai Ma,Tie-Qi Xu
Abstract Soft elastomers are widely used in applications requiring low modulus and resilience, yet their chemical recyclability, particularly the recovery of original monomers to counteract property degradation from repeated thermal processing, remains a significant challenge. To create recyclable soft elastomers, we integrated the chemical recyclability of polyesters with dynamic boronic ester crosslinking chemistry. Through sequential ring-opening polymerization (ROP) of α-methyl-δ-valerolactone (αMeVL) and α,α-spirocyclohexenyl-δ-valerolactone (αSpiroCyEVL), followed by post-modification and crosslinking, a series of soft elastomers with an outstanding service temperature range from –50 to 116 °C were obtained. Introducing δ-valerolactone (δVL) into the soft segment further enabled tunable mechanical properties (modulus of 0.22–6.42 MPa, tensile strength of 0.06–6.40 MPa, elongation at break of 536–1587%, tensile toughness of 0.44–22.0 MJ m–3). The soft elastomers derived from δVL are comparable to a commercial soft polyolefin elastomer. Moreover, these soft elastomers not only exhibit physical recyclability but also enable chemical closed-loop recycling via an ‘alcoholysis–depolymerization–repolymerization’ pathway after multiple physical recycling cycles, demonstrating potential for biomedical devices, flexible electronics, and soft robotics.
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高影响因子
ACS Sensors
·
2024
·
IF 8.2
Linge Nian,Wenhua Li,Chunxia Zhang,Lu Li,Guangrui Zhang,Jianxi Xiao
Hepatic fibrosis, the insidious progression of chronic liver scarring leading to life-threatening cirrhosis and hepatocellular carcinoma, necessitates the urgent development of noninvasive and precise diagnostic methodologies. Denatured collagen emerges as a critical biomarker in the pathogenesis of hepatic fibrosis. Herein, we have for the first time developed 3D-printed collagen capture chips for highly specific surface-enhanced Raman scattering (SERS) detection of denatured type I and type IV collagen in blood, facilitating the early diagnosis of hepatic fibrosis. Employing a novel blend of denatured collagen-targeting peptide-modified silver nanoparticle probes (Ag@DCTP) and polyethylene glycol diacrylate (PEGDA), we engineered a robust ink for the 3D fabrication of these collagen capture chips. The chips are further equipped with specialized SERS peptide probes, Ag@ICTP@R1 (S–I) and Ag@IVCTP@R2 (S–IV), tailored for the targeted detection of type I and IV collagen, respectively. The SERS chip platform demonstrated exceptional specificity and sensitivity in capturing and detecting denatured type I and IV collagen, achieving detection limits of 3.5 ng/mL for type I and 3.2 ng/mL for type IV collagen within a 10–400 ng/mL range. When tested on serum samples from hepatic fibrosis mouse models across a spectrum of fibrosis stages (S0–S4), the chips consistently measured denatured type I collagen and detected a progressive increase in type IV collagen concentration, which correlated with the severity of fibrosis. This novel strategy establishes a benchmark for the multiplexed detection of collagen biomarkers, enhancing our capacity to assess the stages of hepatic fibrosis.
研究领域:
生物医学工程
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分析化学
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肝病学
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分子生物学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Yuan Li,Tong Gao,Ziying Wang,Mengyuan Wang,Xiao Liang,Wenxin Gao,Xiaodan Zhang,Mengni Li,Zongsuo Liang
Abstract Functional oligosaccharides have attracted attention as dietary interventions for type 2 diabetes mellitus (T2DM). However, the effects of oligosaccharides derived from Dendrobium officinale (DOO1) in T2DM remain unclear. This study evaluated the effects of DOO1 on metabolic parameters, gut microbiota, short-chain fatty acids (SCFAs) production, intestinal barrier function, and host metabolism in db/db mice. DOO1 significantly improved glucose and lipid metabolic disorders and reduced pancreatic and hepatic injury. These effects were accompanied by changes in gut microbiota composition, characterized by increased abundances of Duncaniella, Muribaculum, and Paramuribaculum, along with elevated fecal SCFA levels. DOO1 improved intestinal barrier function, as indicated by reduced intestinal permeability and inflammatory markers (TNF-α, IL-1β, LPS), and increased expression of Muc2, ZO-1, and Claudin-1. DOO1 also altered host metabolic profiles and regulated gut-derived hormones and appetite-related neuropeptides. Overall, these findings suggest that DOO1 may alleviate T2DM-related metabolic disorders by modulating gut microbiota and host metabolism.
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高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Fei Zhang,Yingyu Zhang,Xianwei Zhang,Qionglin Wang,Na Zhao,Jianying Ren,Man Zhang,Peng Wang,Mengxin Zhang,Wancun Zhang
Abstract Mitochondrial apurinic/apyrimidinic endonuclease 1 (APE1) mislocalization is closely linked to tumor progression and prognosis. Yet, its ubiquitous distribution across mitochondria, cytoplasm, and nucleus makes specific in situ imaging of mitochondrial APE1 highly challenging. Herein, we fabricate a sequentially activated biosensor (C-12S-tFNA) with 12S rRNA-gated spatial regulation of APE1 cleavage site (apurinic/apyrimidinic site, AP site) accessibility, where cytochrome c (cyt c) acts as the targeting motif to drive precise mitochondrial enrichment. In the initial state, the AP site is structurally locked to prevent nonspecific cleavage by extramitochondrial APE1. After entering the mitochondria, mitochondrial 12S rRNA, which is consistently expressed across diverse cell types, hybridizes with the AP-site-containing strand via base complementarity, leading to AP site unlocking. The exposed AP site is subsequently cleaved by mitochondrial APE1, separating the fluorophore-quencher pair and generating a fluorescent signal. Benefiting from the 12S rRNA-gated design, false-positive signals can be effectively suppressed. Experimental findings demonstrate that C-12S-tFNA exhibits high sensitivity (LOD: 0.002 U/mL) and exceptional specificity for mitochondrial APE1. Notably, it distinguishes high-risk from low-risk neuroblastoma (NB) in vivo without the need for surgically resected or biopsied tissue, while exhibiting favorable biocompatibility. Collectively, C-12S-tFNA enables noninvasive, activity-based mitochondrial APE1 imaging and holds promise for improving precision risk stratification in NB.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Lining Su,Guizhen Wang,Linlin Zhao,Yuan Deng,Yujing Guo,Yong Xiao,Huanwen Wang,Chuan Dong,Lifang Fan
Due to the urgent need for detecting trace amounts of 3,3′,4,4′-tetrachlorobiphenyl (PCB77) in the environment, we have developed an efficient and visible-driven photoelectrochemical (PEC) sensing platform based on carbon quantum dots (CQDs) modified titanium dioxide nanorods (TiO 2 NRs), coupling with exonuclease I (Exo I) assisted in target recycling for significant signal amplification. CQDs/TiO 2 NRs with high visible-light absorption ability and electron-hole separation efficiency is used as photoactive substrate for anchoring anti-PCB77 aptamer and its complementary DNA (cDNA). With the addition of PCB77, the specific interaction between PCB77 and its aptamer forces aptamer to separate from the electrode surface, resulting in an increase in photocurrent density. Adding Exo I in the test system, a self-catalytic target cycle was motivated, which significantly increased the PEC signal by more than twice, achieving signal amplification. The relationship between the photocurrent density changes and the concentrations of PCB77 are utilized to achieve quantitative detection of PCB77. The designed PEC sensing platform has good analytical performance with a detection limit as low as 0.33 pg L −1 , high selectivity and stability. Moreover, the PEC sensor is successfully used to evaluate the content of PBC77 in the environment samples. The established sensing platform provides a simple and efficient method for detecting trace amounts of PCB77 in the environment.
研究领域:
环境科学
·
分析化学
·
光电化学
·
纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Zizhao Fu,Jie Zhao,Rui Jiang,Shiliang Mei,Wanlu Zhang,Ruiqian Guo
I-III-VI alloyed quantum dots (QDs) have shown great application potential in many fields. At present, Al-based alloyed QDs have become a kind of ideal material of the color conversion layer of WLEDs because of their blue-emission, abundant raw material content, and the strong stability of Aluminum oxides. In this study, we synthesized blue-emitting CuAlSe 2 /ZnSe QDs and dual-emitting CuAlGaSe/ZnSe QDs by using hot-injection and one-pot method respectively, with a photoluminescence quantum yield (PL QY) of 46.75% and 68.79%. Their spectral tuning effect and the luminescence mechanism were investigated and discussed by changing the elemental molar ratios and nucleation temperature. By changing the Cu/(Al+Ga) and Al/Ga molar ratio, the emission wavelength of CuAlGaSe/ZnSe QDs was adjustable from 456 nm to 631 nm, with the Al and Ga competition mechanism in CuAlGaSe/ZnSe was also proposed. Finally, a single-component dual-emission CuAlGaSe/ZnSe WLED with a color rendering index (Ra) of 91.4 was prepared. This work demonstrated the superior fluorescence performance of multi-component QDs and the potential application of Al-based QDs in WLEDs.
研究领域:
材料科学
·
纳米技术
·
光电子学
·
固态照明
·
光谱学
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量子点
·
合金半导体纳米晶体
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高影响因子
危险品化学品经营许可证（带存储）