MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-10
·
IF 5.705
Khan Md. Zaved H.,Liu Xiaoqiang,Tang Yunfei,Zhu Jinhua,Hu Weiping,Liu Xiuhua
A glassy carbon electrode (GCE) was modified with poly(L-arginine) (P-Arg), reduced graphene oxide (rGO) and gold nanoparticle (AuNP) to obtain an electrode for simultaneous determination of dopamine (DA), serotonin (5-HT) and L-tryptophan (L-Trp) in the presence of ascorbic acid (AA). The modified GCE was prepared via subsequent ‘layer-by-layer’ deposition using an electrochemical technique. The surface morphology of the modified electrode was studied by scanning electron microscopy, and electrochemical characterizations were carried out via cyclic voltammetry and electrochemical impedance spectroscopy. The modified electrode showed excellent electrocatalytic activity toward DA, 5-HT and L-Trp at pH 7.0. Figures of merit for the differential pulse voltammetric reponse are as follows: (a) Response to DA is linear in two intervals, viz. 1.0–50 nM and 1.0–50 μM DA concentration range, the typical working voltage is 202 mV (vs. Ag/AgCl), and the detection limit is 1 nM (at an S/N ratio of 3). For 5-HT, the respective data are 10 to 500 nM and 1.0 to 10 μM, 381 mV, and 30 nM. For L-Trp, the respective data are 10–70 nM and 10–100 μM, 719 mV, and 0.1 μM. The modified GCE is fairly selective. It was successfully applied to the simultaneous determination of DA, 5-HT, and L-Trp in spiked urine samples, and high recovery rates were found.
Schematic presentation of the voltammetric sensor based on a glassy carbon electrode modified with poly(L-arginine), reduced graphene oxide (rGO) and gold nanoparticle (GCE/P-Arg/ErGO/AuNP) for simultaneous determination of dopamine (DA), serotonin (5-HT) and L-tryptophan (L-Trp).
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jie Cao,Jingge Niu,Yizhuo Pang,Tianshu Luo,Jiayi Peng,Xiaohong Hou
Nitrite is a kind of frequently detected inorganic pollutants in water. In order to sensitively and conveniently monitor the nitrite anytime and anywhere, colorimetric sensing method-based nanoenzymes and portably immediate detection devices become urgent. Herein, we utilized L-arginine as a catalyst and a linker to mediate the synthesis of CeO 2 on bimetallic MOFs (CeO 2 @Mn/ZIF-67), with Co and Mn were set as the central metals. Its enzyme-activated performance ( K m : 0.054 mM and V max : 64.00 μMs −1 ) was superior to most reported materials. Nitrite can diazotize with oxTMB, causing a change in the ratio of absorbance at 652 nm to absorbance at 450 nm (A 652 /A 450 ). Thus, a rapid and sensitive platform for the colourimetric detection of nitrite was established. A portable glass tube hydrogel sensor was designed inspired by the principle of disposable alcohol swabs for the first time to achieve the nitrite monitoring. When the glass tube was cut to introduce TMB and nitrite into the nanoenzymes, a noticeable colour change occurred. Attributed to the high activity of the nanoenzymes and the clever design of the detection device, the proposed method overcame the shortcomings of the traditional sensing methods such as long detection time and poor portability.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Jiaqi Hu,Mingyue Wang,Min Wang,Dandan Wang,Yuqiang Xiao,Yue Lan,Lingling Li,Meiling Yang,Minmin Wang,Leijiao Peng,Famin Ke,Xiurong Guo,Die Gao
Considering the efficient utilization of resources and the simultaneous detection of multiple analytes, achieving selective detection of diverse analytes on one fluorescent covalent organic framework (COF) is of great significance. Herein, a carboxyl-carbazole-rich fluorescent COF (COF-DABT) was strategically synthesized using 3,6-diamino carbazole, capable of forming halogen bonds with analytes, and 2,5-bis(4′-formylphenyl) terephthalic acid, rich in carboxyl groups, which interacts with various analytes and adjusts fluorescence performance via its electron-withdrawing properties. The introduction of carbazole imparts fluorescence to COF-DABT through the intramolecular charge transfer (ICT) effect, while enabling selective fluorescence quenching. Specifically, the ICT of carbazole can be disrupted via linear halogen bond interactions with TBBPS, resulting in selectively detection of TBBPS in a fluorescence-off mode. Furthermore, efficient enrichment of TBBPS is facilitated by halogen bonds with carbazole and hydrogen bonds with carboxyl groups on COF-DABT. Selective fluorescence quenching of COF-DABT by Fe 3 + is achieved through chelating effect around carboxyl group, as well as cation-π interaction with carbazole benzene ring. This enables high selectivity detection of Fe 3+ via a fluorescence-off mode. Additionally, Fe 3+ can be reduced by ascorbic acid through REDOX reaction, mitigating interference with TBBPS detection and facilitating fluorescence-off-on mode detection of AA. Besides, rich carboxyl groups on COF-DABT exert a negative regulatory effect on the carbazole ICT mechanism. To counter this, D/L-Arg, with a size complementary to the adjacent carboxyl groups on COF-DABT, is introduced. Hydrogen bond interactions between D/L-Arg and carboxyl groups reduce the negative regulatory effect, allowing fluorescence enhancement for highly selective detection of D/L-Arg. Through rational design and functional optimization of fluorescent COF structures, multi-mode detection methods enable simultaneous detection of different analytes, achieving the goal of "one stone, several birds."
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2019
·
IF 3.743
Gang Zheng,Meizhu Zheng,Ben Yang,Hui Fu,Yongqing Li
Breast cancer is the leading cause of cancer mortality in women worldwide. To overcome the toxic side effects and multidrug resistance (MDR) during doxorubicin (DOX) chemotherapy, an arginine-glycine-aspartic (RGD) tripeptide modified, pH-sensitive solid lipid nanoparticles (SLNs) is employed in this study. In this study, a RGD conjugated, pH sensitive lipid was synthesized using glycerin monostearate (GMS) and adipic acid dihydrazide (HZ) as lipid materials and named RGD-HZ-GMS. RGD-HZ-GMS was applied to encapsulate DOX to construct a RGD modified, DOX loaded SLNs (RGD-DOX-SLNs). To evaluate the anticancer effect of RGD-DOX-SLNs, breast cancer cell line (MCF-7 cells) and DOX resistant cell line (MCF-7/ADR cells) were used. in vivo tumor suspension and toxicity effects were evaluated on mice bearing MCF-7/ADR cells breast cancer model. RGD-DOX-SLNs had a uniformly spherical shape. The mean particle size and zeta potential of the RGD-DOX-SLNs was 96.3 nm and 35.6 mV, respectively. RGD-DOX-SLNs showed 5.58 fold higher area under the plasma concentration – time curve (AUC) compared with DOX solution. Terminal half life (T 1/2 ) and peak concentration (C max ) of RGD-DOX-SLNs was 10.85 h and 39.12 ± 2.71 L/kg/h. in vitro and in vivo antitumor results indicate that RGD-DOX-SLNs might be a promising novel lipid carrier which could improve breast cancer therapy.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
·
2020
·Vol. 53
·期 1
·133-150
·
IF 1.451
Dahai Gu,Jingying Zhao,Samwel Mchele Limbu,Yunan Liang,Junming Deng,Baoliang Bi,Lingfu Kong,Hui Yan,Xiaowen Wang,Qing Hu,Yu Gao,Dan Jia,Hua Rong
Arginine (Arg) is an important essential amino acid required for optimum fish growth. However, its role in plant-rich diets on growth and the required level in juvenile Hemibagrus wyckioides are currently unknown. This study evaluated the effects of Arg supplementation in plant-rich diets on growth, feed utilization, whole body composition, blood biochemical indicators and gene expressions of the target of rapamycin (TOR) signaling pathway in juvenile H. wyckioides . A total of 450 fish (6.40 ± 0.06 g) were randomized into six equal groups and fed on diets with graded Arg levels: 0 (control), 3, 6, 9, 12 and 15 g/kg of dry feed for eight weeks. The results revealed that specific growth rate (SGR) and feed conversion ratio of juvenile H. wyckioides were significantly improved and reduced, respectively in fish fed on 9 g/kg of Arg supplementation ( p < .05). The optimum amount of dietary Arg content in plant-rich diets for H. wyckioides was 63.77 g/kg diet based on SGR. Feeding fish with dietary Arg supplementation ranging from 9 to 12 g/kg improved significantly ( p < .05) whole body protein contents and decreased lipid deposition. The mRNA levels of TOR, eukaryotic translation initiation factor 4E (4EBP) and ribosomal protein S6 kinase 1 (S6K1) in muscle and liver were significantly affected by increasing dietary Arg levels. Meanwhile, the mRNA relative expression levels of TOR and S6K1 in the liver and muscle increased significantly as Arg supplementation increased. On the other hand, the mRNA relative expression levels of 4EBP was significantly higher in fish fed on the 9 g/kg Arg diet compared to those fed on the control and 15 g/kg Arg diets. In conclusion, our current results indicate that dietary Arg supplementation in plant-rich diets, might activate the TOR signaling pathway, which promotes protein synthesis and reduces lipid accumulation of H. wyckioides , resulting in improved growth.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）