FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Jingnan Zang,Mingmin Qing,Yujie Chi,Yuan Chi
This study focused on understanding the synergistic effects of ultrasound and amino acids (arginine and lysine) in delaying the heat-induced protein aggregation process of liquid egg whites (EW) and enhancing gel characteristics, as well as the impact of the sequence of these treatments. The results indicate that the amino acid-only group had a limited effect in preventing thermal aggregation, but it could enhance the gumminess and chewiness of egg white gels. Compared to the control group CH, the group that first received lysine followed by ultrasound treatment (LU) exhibited superior gel properties, with improvements of approximately 14.35% in hardness, 36.96% in chewiness, 31.77% in gumminess, 15.76% in cohesiveness, and 36.87% in resilience. EW treated with arginine followed by ultrasound (AU) demonstrated greater potential in enhancing gel water-holding capacity, cohesiveness, and elasticity. In contrast, the LU exhibited lower turbidity, particle size, and gel color difference while showing higher gel hardness. The ultrasound treatment applied after amino acids accelerated the unfolding of protein structures, leading to enhanced hydrophobic interactions and disulfide bonds, as well as an increased β-sheet content, resulting in a more regular and ordered gel structure with tighter packing and uniform dispersion. These findings suggest that the combination of arginine and lysine with ultrasound is an effective strategy to delay thermal aggregation during the thermal sterilization process, while also enhancing the textural properties of the gel.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2016
·Vol. 163
·期 14
·B744
·
IF 3.014
Qiao Xia,Xia Lin,Chengcheng Yang,Jiao Ma,Yingzi Fu
Poly(glutathione disulfide)-poly(L-lysine) (PGSSG-PLY) multilayer films prepared by electropolymerization via cyclic voltammetry were constructed as a new chiral interface for recognition of ascorbic acid (AA) and isoascorbic acid (IAA). The morphology of the films was characterized by scanning electron microscopy (SEM), the electrochemical behaviors of the films were studied via chemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Meanwhile, differential pulse voltammetry (DPV) was used to study the interactions between PGSSG-PLY modified glassy carbon electrodes and AA or IAA. A strong interaction with AA was observed on the chiral interface. The results proved that PGSSG film could amplify eletrochemical signal, while PLY film could provide more recognition sites to discriminate AA and IAA enantiomers with satisfying recognition efficiency. The possible formation of the films on the electrode surface was described. And the influences of electropolymerization cycles and pH to the recognition process were explored. Under the optimum conditions, the method showed an acceptable linear response to AA and IAA in a range from 1.0 × 10−7 to 5.0 × 10−3 M with a low detection limit of 3.3 × 10−8 M (S/N = 3). The modified electrodes also displayed good stability and reproducibility.
研究领域:
分析化学
·
生物化学
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Analytical Methods
·
2015
·Vol. 8
·期 2
·453-459
·
IF 1.821
Hong Zhi Zhang,Rong Sheng Li,Ni Wang,Li Qi,Cheng Zhi Huang,Jian Wang
Sensing biomolecules such as heparin sodium and lysine are of great significance. In this work, a “turn-off–on” fluorescence switching of cadmium telluride quantum dots (CdTe QDs) was designed for both heparin sodium and lysine. Even though both heparin sodium and thioglycolic acid (TGA)-capped CdTe QDs are negatively charged, they are capable of forming a self-assembly and even aggregates through hydrogen bonding, depending on the concentration of heparin sodium, which leads to the sensitive fluorescence quenching of CdTe QDs. Thus, a fluorescence ‘turn-on’ analytical method for heparin sodium sensing could be established with a detection range of 0.200–5.000 μg mL−1 and the detection limit was 0.033 μg mL−1, which is applicable to determining heparin sodium in injection samples. What's more, a sensitive and selective “turn-off–on” nanosensor was developed for lysine analysis with the detection range of 2–200 μmol L−1 and the detection limit as low as 0.146 μmol L−1. This approach offers a new, simple, fast and selective method for determining heparin sodium and lysine.
研究领域:
分析化学
·
生物医学
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Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Yiyan Song,Ping Zhu,Yuan Wu,Li Tan,Wei Wei,Songqin Liu,Qing Huang,Jin Chen
The emergence of drug-resistant fungal strains remains a severe threat for the public health, which prompts strict restrictions on the uses of antifungal drugs. However, the majority of lipophilic fungistatic agents are poorly water soluble with a low oral adsorption characteristic posing challenges for the precise prescriptions. In this study, a natural antimicrobial cationic peptide of epsilon-poly- l -lysine (EPL) decorated ordered mesoporous silica (SBA-15) was facilely prepared for the efficient loading of antifungal itraconazole (ITZ) drugs. The characterized mesoporous SBA-15/EPL/ITZ composite exhibited remarkable antifungal performance against Aspergillus fumigatus as a model mold, which was attributed to synergistic antifungal activities of ITZ and EPL in the mesopores. Moreover, the in vitro release behaviors of ITZ in the composite nanoexcipients both in simulated gastric fluid and fasted state simulated intestinal fluid were studied. The observed release kinetics of ITZ demonstrated a contributing role of SBA-15/EPL to enhance the solubility of ITZ and thereby may promote its flux across the gastrointestinal epithelium, which is beneficial for the absorption of drugs. Additionally, SBA-15/EPL/ITZ composites showed desirable biocompatibility toward mammalian red blood cells, human cervical cancer cells (Hela) and human embryonic kidney cells (HEK-293T). Furthermore, the pharmacokinetic profiles of obtained nano-formulations were assessed in rats, among which the improved adsorption of SBA-15/EPL/ITZ composites (AUC 0–24h sum : 8381.7 nM·h) was identified compared with that of pure ITZ (525.1 nM·h) and the commercial drug of Sporanox (7516.6 nM·h). Collectively, the prepared SBA-15/EPL/ITZ provides an ecofriendly and integrated nanocomposite with enhanced solubility of lipophilic drugs to combat proliferations of infectious fungi.
研究领域:
药剂学
·
生物材料
·
微生物学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Hang Yu,Qili Zhong,Yunfei Xie,Yahui Guo,Yuliang Cheng,Weirong Yao
A kinetic model for Maillard reaction (MR) model system of d -glucose and l -lysine was established; activation energy ( Ea ) of each step was calculated. Potential generation pathways of furosine and pyrraline were a combination of either 3-deoxyglucosone (3-DG) or methylglyoxal (MG) with l -lysine. Ea value for furosine generated through 3-DG pathway was 81.70 ± 14.01 kJ mol −1 , which was significantly higher than that through MG pathway (52.08 ± 4.48 kJ mol −1 ). As for pyrraline, Ea for the 3-DG pathway (53.45 ± 4.02 kJ mol −1 ) was significantly lower than that through the MG pathway (110.22 ± 18.77 kJ mol −1 ). Results of the kinetic study indicated that furosine was preferred to be generated through the MG pathway since MG is more likely to react with each other and form a furan ring as a precursor of furosine. Pyrraline was more easily to be generated from the 3-DG pathway through cyclization of 1,4-dicarbonyl compounds to pyrrole.
研究领域:
食品科学
·
生物化学
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-9
·
IF 5.479
Liu Mingwang,Zhou Ji,He Yu,Cai Zhaoxia,Ge Yili,Zhou Jiangang,Song Gongwu
Titanium carbide quantum dots functionalized with ε-poly-L-lysine (PLL) were synthesized by sonication cutting and hydrothermal synthesis. The deprotonated Ti 3 C 2 MXene quantum dots (Ti 3 C 2 MQDs) exhibit excitation wavelength-dependent blue photoluminescence with typical excitation/emission peaks at 330/415 nm and a quantum yield of 22% due to strong quantum confinement. The fluorescence of ε-poly-L-lysine protected Ti 3 C 2 MQDs (PLL-protected Ti 3 C 2 MQDs) is reduced via an inner filter effect after the addition of cytochrome c (cyt-c). Response to cyt-c is linear in the 0.2 to 40 μM concentration range and the detection limit is 20.5 nM. In the presence of trypsin, cyt-c is hydrolyzed to small peptides, and the Fe 3+ ion in cyt-c probably is reduced to Fe 2+ with the aid of the digestive enzyme. This results in the restoration of the blue fluorescence of the modified MQDs. Fluorescence increases linearly in the 0.5 to 80 μg mL −1 trypsin concentration range with the detection limit of 0.1 μg mL −1 . The method was successfully applied to the determination of cyt-c and trypsin in spiked serum samples.
Schematic of a method for the fluorometric “turn-off-on” determination of cytochrome c and trypsin based on ε-poly-L-lysine (PLL) protect MXene quantum dots (Ti 3 C 2 MQDs).
研究领域:
材料科学
·
分析化学
·
生物传感器
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PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 6
·855
·
IF 5.561
Xiuping Li,Wenhui Wang,Shouyin Wang,Yuqing Shen,Jinfeng Pan,Xiuping Dong,Shengjie Li
This study aimed to investigate the presence ofL-lysine (Lys) on the solubility and structures of myofibrillar proteins (MFPs) at different ionic strengths. Porcine MFPs were incubated at 4 °C with various levels of ionic strengths (0.15, 0.3, or 0.6 M NaCl) with or without the presence of 20 or 40 mM Lys. After 24 h of incubation, MFP solubility and turbidity were determined, and the particle size distribution, circular dichroism spectra, and intrinsic tryptophan fluorescence of MFP were analyzed to obtain their secondary and tertiary structure. Results showed that the solubilization effects of Lys on MFPs are dependent on the ionic strength. Particularly, the presence of Lys could improve MFP solubility at 0.3 M, which resembles salt-reducing processing conditions. Concomitantly, the secondary and tertiary structures were observed to change as a result of the varying ionic strengths and the addition of Lys, including myofibril swelling, dissociation of myosin filaments, uncoiling of α-helix, and unfolding of the tertiary structure. The possible mechanisms underlying the solubilization effects of Lys on MFPs at low ionic strengths are discussed from the perspective of protein structural changes.Keywords:myofibrillar proteins;L-lysine;solubility;conformation;low salt
研究领域:
食品科学
·
蛋白质化学
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高影响因子
JOURNAL OF DAIRY SCIENCE
·
2022
·
IF 4.225
Shuangyan Ding,Hailiang Hu,Xianglin Yue,Kaiwen Feng,Xiaoyu Gao,Qiuling Dong,Mingqi Yang,Ugur Tamer,Ganhui Huang,Jinsheng Zhang
ABSTRACT Salmonella , as a common foodborne pathogen in dairy products, poses a great threat to human health. We studied a new detection method based on quantum dots (QD). A fluorescent biosensor with multiple fluorescent signal amplification based on a streptavidin (SA) biotin system and the polyamino linear polymer poly- l -lysine (PLL) were established to detect Salmonella in milk. First, Salmonella was captured on a black 96-well plate with paired Salmonella mAb to form a double-antibody sandwich. Second, SA was immobilized on biotin-modified mAb by SA-biotin specific bond. Then, the biotin-modified polylysine (BT-PLL) was bound on SA and specifically bonded again through the SA-biotin system. Finally, water-soluble CdSe/ZnS QD-labeled SA was added to a black 96-well plate for covalent coupling with BT-PLL. The fluorescent signal was amplified in a dendritic manner by the layer-by-layer overlap of SA and biotin and the covalent coupling of biotinylated PLL. Under optimal conditions, the detection limit was 4.9 × 10 3 cfu/mL in PBS. The detection limit was 10 times better than that of the conventional sandwich ELISA. In addition, the proposed biosensor was well specific and could be used for detecting Salmonella in milk samples.
研究领域:
食品科学
·
生物技术
·
分析化学
·
微生物学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Feng Gao,Jinghuan Fu,Siyuan Song,Yuhang Yao,Zeyu Guo,Xingyu Wang,Fei Jin,Ying Wang,Gongxun Bai
Surface and interfacial regulation is critical for breathable wearable materials that require stable humidity-responsive electrical output together with auxiliary thermal functionality. Herein, we report a lysine-stabilized MXene/polyvinyl alcohol (PVA) electrospun nanofibrous membrane in which Ti₃C₂Tₓ MXene is incorporated into a porous and vapor-permeable fiber network. This design promotes interfacial compatibility between MXene and PVA, improves its dispersion within the polymer matrix, and preserves a breathable porous architecture that favors water-vapor transport and interfacial moisture uptake. The 2 mg/mL MXene-Lys/PVA exhibits a strong humidity-dependent electrical response, delivering a resistance response of 10,665 over 40–90% RH, and stable operation during repeated cycling and two months of storage. The sensing behavior is mainly associated with water-adsorption-mediated modulation of charge transport within the hydrated polymer/MXene interfacial network, while an additional low-frequency interfacial process emerges under high-humidity conditions. Beyond humidity sensing, the composite largely retains the vapor-transport capability of the porous PVA matrix and simultaneously provides effective photothermal conversion under light irradiation. A garment-mounted PET-ITO prototype further enables real-time wireless transmission of activity-associated humidity-responsive signals. These results show that L -lysine-assisted MXene integration in electrospun porous membranes provides a composition-tunable platform for humidity-responsive electrical readout and photothermal heating demonstrations. Download: Download high-res image (187KB) Download: Download full-size image
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BIOCHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 3.6
Wei Tang,Haojie Qian,Xinyue Wang,Jiajia Wang,Jiaming Zhang,Caoxing Huang,Bo Fan,Yucai He
Amino acid-based deep eutectic solvents (DES) have emerged as a quintessential class of alkaline DES, known for exceptional delignification in lignocellulose pretreatment under mild conditions, thereby enhanced the economic feasibility of biorefinery. This study introduces an innovative ternary DES comprised of cetyltrimethylammonium bromide (CTAB), ethanolamine (EA), and lysine (Lys), engineered for efficient pretreatment of poplar sawdust (PS). Results show that increased Lys concentration progressively reduces pH, conductivity, and polarity, while significantly enhancing hydrogen bond donor and acceptor capacities. The amino and carboxyl groups in Lys synergistically establish a robust three-dimensional hydrogen-bonded network. Specifically, DES pretreatment dramatically improves PS fractionation: compared with the Lys-free control, Lys introduction elevates lignin removal from 42.2% to 87.2%, and xylan removal from 78.5% to 99.9%, respectively. Meanwhile, cellulose digestibility increases from 65.5% to 96.0%, and ethanol yield rises from 56.3% to 89.9%. Structural characterization unveiled that Lys remarkably promoted selective cleavage of lignin linkages, thereby dismantling the lignin framework. This reorganization reduces lignin’s specific surface area, enhances cellulose exposure, and boosts enzymatic hydrolysis. Notably, the DES exhibits excellent recyclability. These findings highlight the great potential of alkaline DES for sustainable biorefining and valorization of forestry biomass residues.
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Biomaterials Science
·
2023
·Vol. 11
·期 19
·6504-6523
·
IF 6.6
Shuying Zhao,Meiqi Fang,Yan Li,Fujun Wang,Hao Li,Lu Wang
Polypropylene (PP) sling implantation is the most commonly performed procedure for women with stress urinary incontinence (SUI). However, concerns have arisen regarding complications caused by slings, including the common issue of erosion, which can be attributed to various factors such as the body's response and bacterial contamination. To address these concerns, we have developed a rectangular mesh self-locking edge sling with a large pore size and lightweight design. Promising results have been obtained from preliminary in vivo mechanical reliability tests, including uniaxial tensile tests. In comparative in vitro fixed load tensile tests and simulated Tension-free Vaginal Tape (TVT) and Transobturator Vaginal Tape inside-out (TVT-O) technique tests using commercial slings, our sling demonstrated less transverse wrinkling. Both slings achieved an effective porosity of over 45% under the TVT technique. However, the commercial sling experienced a significant reduction in effective porosity during the TVT-O technique, whereas our sling maintained a stable effective porosity with minimal wrinkling. Furthermore, we successfully developed cationic hydration rejection-driven antibacterial-anti-fouling coatings on the surface of our sling by grafting hyperbranched poly-lysine (HBPL) mediated by polynorepinephrine. The HBPL coating imparts a positive charge and hydrophilicity to the sling, resulting in elevated bactericidal activity and reducing protein adhesion. An optimal grafting concentration of 20 mg mL−1 was selected, confirming the stability and biocompatibility of the sling coating. This coating is expected to reduce the likelihood of postoperative erosion. Overall, our research represents significant advancements in improving the safety and performance of PP slings for stress urinary incontinence, potentially leading to a reduction in complications following surgery.
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 4.446
Chenyang Qi,Yipin Zhang,Jing Tu
Nanomaterial-based antimicrobial strategies have emerged as powerful tools to combat microbial resistance, avoiding side effects of misusing antibiotics. In this work, the zinc oxide-based nanohybrid (designated as [email protected] ε-PL) as broad-spectrum antibacterial agents were fabricated in a facile two-step process. To be specific, ZnO was coated with near-infrared (NIR)-photoresponsive polydopamine (PDA), and then coupled with the antimicrobial peptide (AMP) (ε-poly- L -lysine, ε-PL). The as-prepared [email protected] ε-PL can effectively contact and capture bacteria, and then exhibit an excellent photothermal effect, i.e . , the temperature rises rapidly to 53.2 °C (100 μg/ml) under 808 nm NIR light irradiation for 10 min. The synergistic effect of ε-PL, ZnO and photothermal agent PDA contributes to the excellent antibacterial efficiency of [email protected] ε-PL (<100 μg/ml) with 808 nm irradiation, that is, 99.70% and 100.00% against Gram-negative bacteria ( Escherichia coli , E. coli ) and Gram-positive bacteria ( Staphyloccocus aureus , S. aureus ), respectively. Further experiments indicated the [email protected] ε-PL ultimately lead to bacterial death by disrupting cell integrity, leaking of intracellular components (e.g., nucleic acids). Moreover, [email protected] ε-PL can effectively remove 70.84% of S. aureus biofilms. Overall, NIR-responsive [email protected] ε-PL has great potential in the field of infection treatment.
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