Biomed Research International
·
2022
·
IF 3.246
Jie Jiang,Zhenghua Liu,Xiuping Zhou,Fenglan Peng,Zhihui Wang,Feng Li,Ming Li
To investigate the effect and mechanism of resveratrol on the biological behavior of cervical cancer cells (HeLa cells), the apoptosis, migration, and invasion of HeLa cells were detected by flow cytometry, wound healing, and transwell assays. The expression levels of Hedgehog signal pathway proteins (smoothened (SMO), zinc finger transcription factors (Gli1), and sonic hedgehog homolog (Shh)) were detected by quantitative real-time PCR (qPCR) and western blotting. Compared with that control group, resveratrol (RES) significantly induced apoptosis, inhibited the migration and invasion of the HeLa cells. The expression of SMO, Gli1, and Shh were downregulated in the HeLa cells treated with RES. The Hedgehog agonist purmorphamine (PUR) reversed the RES-induced increase of apoptosis and reduction of migration and invasion in the HeLa cells. In conclusion, RES induced the apoptosis and suppressed the migration and invasion of HeLa cells by inhibiting Hedgehog signal pathway.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Lin Bai,Rundong Liu,Ruike Wang,Yongjuan Xin,Zuntao Wu,Yue Ba,Huizhen Zhang,Xuemin Cheng,Guoyu Zhou,Hui Huang
This study examined the neuroprotective properties of resveratrol (Res) and its target sirtuin1 (SIRT1) against lead (Pb)-mediated toxicity and discovered that both resveratrol treatment and SIRT1 overexpression restored blocked autophagic flux as well as reduced β-amyloid (Aβ) contents. Four-week-old male C57BL/6 mice were employed to consumed 0.2% Pb(Ac) 2 solution or deionized water for 3 months followed by 12 months of Res (50 mg/kg BW) or vehicle gavage. In in vitro study, SH-SY5Y cells were pretreated with the SIRT1 activator SRT1720 (2 μM) or the inhibitor EX527 (2 μM) for 2 h, then 25 μM of Pb(Ac) 2 was added and incubated for 48 h. Western blotting, RT-qPCR, enzyme-linked immunosorbent assay (ELISA), and Lyso-Tracker Red Staining were next used to estimate the potential alterations of the autophagic pathway as well as BACE1-mediated amyloid processing in response to Pb exposure, respectively. Our data revealed that Res treatment or SIRT1 activation resisted the induction of autophagy by Pb exposure through inhibition of LC3 and Beclin-1 expression and promoted the degradation of Aβ and Tau phosphorylation. Besides, the SIRT1 activator (SRT1720) downregulated the expression of BACE1, the rate-limiting enzyme for Aβ production, by inhibiting the activation of nuclear factor-κB (NF-κB) in Pb-treated SH-SY5Y cells, which resulted in reduced Aβ production. Collectively, we verified the role of Res-SIRT1-autophagy as well as the SIRT1-NF-κB-BACE1 pathway in Pb-induced neuronal cell injury by in vivo or in vitro models. Our findings further elucidate the important role of SIRT1 and Res in counteracting Pb neurotoxicity, which may provide new interventions and targets for the subsequent treatment of neurodegenerative diseases.
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Journal of Functional Foods
·
2025
·
IF 4
Lifang Feng,Tianyu Zhang,Wei Ye,Daofeng Qu
Resveratrol and rutin, two polyphenolic compounds, can effectively alleviate the symptoms of ulcerative colitis (UC), leading to conflicts in recommending resveratrol- or rutin-rich diets to UC patients, who do not know which type of diet is more effective in alleviating UC symptoms. In this study, administration of resveratrol or rutin alleviated DSS-induced UC mice, including reduction of DAI, serum levels of inflammatory cytokines and oxidant, and improvement of colonic health status. Differentially expressed genes resulting from resveratrol and rutin treatment in DSS-induced UC mice at the genome-wide level from scratch and elucidated the mechanism by which they affected the polarization of intestinal epithelial T cells by promoting Treg cells and inhibiting Th17 cells to alleviate UC symptoms, and rutin was greater than resveratrol. In addition, rutin-rich foods were superior to resveratrol-rich foods in ameliorating UC symptoms in mice. Our findings suggest that UC patients consume rutin-rich foods to better alleviate intestinal inflammation.
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RSC Advances
·
2025
·Vol. 15
·期 25
·19786-19801
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IF 4.6
Zhijie Liang,Jinzhuai Li,Shuying Luo,Shaorong Li,Kun Zhao,Hongmian Jiang,Yisi Ou,Juan Zhong,Lifeng Luo,Huali Huang,Yingying Li
Resveratrol (RSV), a natural polyphenol with potent antioxidant and anti-inflammatory properties, exhibits significant therapeutic potential for non-alcoholic fatty liver disease (NAFLD) by modulating lipid metabolism, oxidative stress, and inflammatory pathways. Despite its multi-target mechanisms and enhancement of reverse cholesterol transport, clinical translation remains limited by poor bioavailability and inefficient intracellular delivery. Conventional oral administration fails to achieve therapeutic plasma concentrations owing to extensive first-pass metabolism and low solubility. To address these limitations, this study developed galactose (Gal)-modified lipid nanoparticles (Gal-LNPs) to enhance hepatic targeting via asialoglycoprotein receptor-mediated endocytosis. These Gal-LNPs demonstrated significantly improved intracellular RSV delivery (3.49-fold uptake vs. unmodified LNPs). In NAFLD mouse models, Gal-LNP-RSV reduced hepatic lipid accumulation and serum alanine aminotransferase/aspartate aminotransferase levels by 48.3% and 58.7%/49.3%, respectively, outperforming free RSV in both aspects. These findings underscore the potential of Gal-LNPs as a transformative means of overcoming RSV's pharmacokinetic barriers, enabling the precise activation of intracellular targets while restoring metabolic and redox homeostasis. This work provides a robust framework for developing targeted nanotherapeutics against NAFLD, bridging the divide between preclinical efficacy and clinical application.
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HISTOLOGY AND HISTOPATHOLOGY
·
2026
·Vol. 41
·期 3
·453-465
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IF 2.2
Xuefei Fan,Suhuan Chen,Haomiao Liu,Nannan Liu,Mengyan Zhang,Mengmeng Chen,Wuqi Chen,Guangyi Chen,Weilu Gao,Tao Yao,Xiaoyu Chen
Background: Rheumatoid arthritis can affect extra-articular organs such as the liver, and the problem of drug-induced liver injury caused by traditional antirheumatic drugs for improving the condition cannot be ignored. This study aims to investigate the therapeutic effects of resveratrol (Res) on hepatic inflammation and oxidative stress in mice with collagen-induced arthritis (CIA) and to elucidate the relationship between the regulatory mechanisms of the nuclear factor erythroid 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) signaling pathway. Methods: In this study, we used chicken type II collagen in combination with complete Freund's adjuvant to induce arthritis in a mouse model, and Res was administered by tube feeding to detect the serum biochemical liver function and inflammation levels, oxidative stress, and apoptosis in the livers of mice. An in vitro cellular model of liver inflammation and oxidative stress was established by treating mouse primary hepatocytes (MPHs) with tumor necrosis factor-α (TNF-α) and H 2 O 2 . The intrinsic mechanism of Res in attenuating hepatic inflammation and oxidative stress in CIA mice was explored by treating MPHs with an Nrf2 inhibitor and Keap1 overexpression plasmid. Results: Res significantly reduced the levels of inflammation and oxidative stress in liver tissues of CIA mice as well as in MPHs treated with TNF-α and H 2 O 2 and activated the Nrf2/Keap1 signaling pathway. Inflammation and oxidative stress levels in MPHs were exacerbated by the use of Nrf2 inhibitors and Keap1 overexpression, which promoted apoptosis. Conclusion: This study demonstrated the intrinsic mechanism of Res of attenuating hepatic inflammation and oxidative stress in CIA mice through the Nrf2/Keap1 pathway, which provides a new idea for finding hepatoprotective treatments for rheumatoid arthritis.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Liling Fu,Yanan Ren,Conghe Zhang,Wei Yang,Qisheng Tian,Guixiang Zhou,Tian Xu,Changhong Liu,Lei Zheng
In this study, n-butyric acid-modified high-amylose corn starch (NBA-HACS) with varying degrees of substitution (DS ≈ 0.1, 0.2, 0.3) was synthesized via carbodiimide-mediated esterification and employed to fabricate Pickering emulsions for resveratrol encapsulation and delivery. Structural characterization confirmed successful grafting of n-butyric acid onto the C-6 hydroxyl groups of native high-amylose corn starch (NA-HACS), enhancing particle hydrophobicity. The contact angle increased from 24.6° (NA-HACS) to 86.3° (NBA-HACS), indicating a pronounced enhancement in particle dual wettability. NBA-HACS3 formed stable Pickering emulsions at 10% (w/v) concentration and oil-to-water ratios of 4:6 and 5:5, maintaining structural integrity across pH 1–11 and remaining stable after 90 days of storage. NBA-HACS3 achieved high resveratrol encapsulation efficiency (90.3%) and exhibited excellent retention under thermal (37 °C, 35 days) and UV conditions (11 h ). In vitro digestion studies revealed that NBA-HACS3 emulsions enhanced resveratrol bioaccessibility to 38%, five times higher than the control. This resulted from efficient interfacial encapsulation enabled by increased hydrophobicity, enhanced acid resistance preserving droplet integrity under gastric conditions, and improved micellar solubilization during intestinal digestion. This establishes an effective modification strategy for enhancing starch-based particle emulsifying performance, offering a promising food-grade delivery system for hydrophobic bioactives in functional food applications.
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Biomaterials Advances
·
2026
·
IF 6.7
Ling Peng,Yusong Pan,Jun Tao,Cuilian Wei,Qianqian Liu,Run Huang,Xiuling Lin,Rui Yue
To address core issues in spinal cord injury repair such as oxidative stress, excessive inflammation and impaired nerve regeneration, this study developed a 3D-printed multifunctional PCP-Res-NGF scaffold. The scaffold achieves stable loading of resveratrol and nerve growth factor by sequentially coating chitosan and polydopamine on the polylactic acid skeleton. Experiments show that the scaffold has pH/ROS dual-responsive drug release characteristics, fitting the S-type Weibull model in simulated injury environments and following a first-order kinetic model under physiological conditions; it exhibits significant scavenging capabilities against DPPH, ·OH, and H 2 O 2 , effectively reducing intracellular ROS levels, an effect attributed to the radical quenching properties of polydopamine and the dual action of resveratrol. By inhibiting the NLRP3 inflammasome, the scaffold shifts lipopolysaccharide-stimulated macrophages from the pro-inflammatory M1 phenotype toward the anti-inflammatory M2 phenotype, as evidenced by reduced iNOS expression (from 19.62 to 3.43) and increased ARG-1 expression (from 2.06 to 6.04). Furthermore, the scaffold promotes the differentiation of injured PC12 cells into MAP2-positive neurons. In the PCP-Res-NGF (LPS-PR) group, the neurite length reached 119.59 μm, representing a 13.26% increase compared to the LPS-only control group. This scaffold provides a promising therapeutic platform for spinal cord injury repair through synergistic antioxidant, anti-inflammatory and neurodifferentiation-promoting effects.
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PHYTOMEDICINE
·
2026
·
IF 11.3
Quanyong Wu,Boya Ouyang,Wanning Ma,Yuxuan Zhao,Jingyimei Liang,Yi Yuan,Hui Cao,Jesus Simal-Gandara,Shaoping Nie,Jianbo Xiao
Background Resveratrol is a polyphenolic compound with reported anti-aging properties that exhibit complex and context-dependent biological effects, while its potential multigenerational influence remains unclear. Objectives To investigate the stage-specific effects of resveratrol on development and lifespan in Drosophila melanogaster and to determine whether resveratrol-associated longevity benefits can extend across generations through metabolic and epigenetic regulation. Methods Drosophila melanogaster were exposed to 50 μg/mL resveratrol (ER50) either during embryonic development or during adulthood. Developmental timing, lifespan, hormonal and nutrient-sensing signaling pathways, stress-response and autophagy pathways, mTOR signaling, metabolomic profiles, histone modifications, histone-modifying enzyme expression, and multigenerational lifespan effects were evaluated. Results Embryonic exposure to ER50 accelerated pupation and adult emergence but shortened adult lifespan. These effects were accompanied by activation of the juvenile hormone–insulin/IGF signaling axis and impaired antioxidant capacity. In contrast, adult ER50 supplementation extended lifespan in female flies and was associated with enhanced stress-response and autophagy pathways together with suppression of mTOR signaling. Mechanistically, longevity-promoting effects in adults coincided with increased glycolysis and pentose phosphate pathway activity, citrate accumulation, and remodeling of one-carbon metabolism, indicating broad reprogramming of energy and redox metabolism. These metabolic changes were accompanied by increased H3K9 acetylation (H3K9ac), upregulation of the histone acetyltransferase-related factor nej , and downregulation of Ash1 , suggesting that resveratrol promotes lifespan extension through coordinated regulation of metabolic state and chromatin remodeling. Notably, maternal adult exposure to ER50 was associated with lifespan extension in female offspring across two subsequent generations, together with persistent metabolic alterations and chromatin changes. Conclusions Resveratrol exhibits stage- and sex-dependent effects in Drosophila and functions as a metabolic–epigenetic modulator. Adult supplementation is associated with extended female lifespan and multigenerational longevity effects, potentially mediated through coordinated regulation of energy metabolism and chromatin homeostasis. Download: Download high-res image (181KB) Download: Download full-size image
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MOLECULES
·
2023
·Vol. 28
·期 2
·737
·
IF 4.6
Wenling Fan,Jiali Wu,Meiqi Gao,Xiaotong Zhang,Wenjing Zhu
The aim of this study was to improve the solubility, bioavailability, and stability of resveratrol (RES-SD) Solid Dispersion inPolygonum cuspidatumextract (PCE) by hot melt extrusion (HME). In addition, the role of the auxiliary substances in PCE was also studied. The solid dispersion ofPolygonum cuspidatumextract was prepared by hot-melt extrusion. The optimum formula was selected by single factor design and orthogonal test. The optimum formula was barrel temperature 140 °C, screw rotation speed 40 rpm/min, and the ratio ofPolygonum cuspidatumextract to HPMCAS was 1:2. The dissolution test showed that PCE-SD increased the dissolution of RES from 46.75 ± 0.47% to 130.06 ± 0.12%. The pharmacokinetics curve of rats showed that PCE-SD increased AUC0-t of RES from 111,471.22 ± 11.4% to 160,458.968 ± 15.7%, indicating an approximately 1.44-fold increase in absorption. In addition, the rotation speed of PCE-SD screw is less than that of RES-SD screw. The bioavailability of PCE-SD was slightly better than that of RES-SD. PCE-SD is more hygroscopic than RES-SD. PCE-SD increased the solubility and oral bioavailability of RES. The auxiliary substances in Polygonum cuspidatum extract have influence on its preparation technology, stability, and bioavailability.Keywords:resveratrol;polygonum cuspidatum extract;hot-melt extrusion;solid dispersion;attendant substances
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Junfeng Zheng,Rui Zhao,Adam A. Uliana,Yanyan Liu,Damien de Donnea,Xin Zhang,Daliang Xu,Qieyuan Gao,Pengrui Jin,Yanlin Liu,Alexander Volodine,Junyong Zhu,Bart Van der Bruggen
A precise separation of organic dyes and inorganic salts is a prerequisite for zero liquid discharge of saline textile wastewater. The development of loose nanofiltration (LNF) membranes with customized nanoscale pores and high water permeance is expected to address this challenge. Herein, a new LNF membrane is reported, fabricated via a scalable interfacial polymerization (IP) approach between resveratrol and trimesoyl chloride (TMC) on a Kevlar substrate. The optimal resveratrol/TMC membrane exhibited an ultra-high pure water permeance (121.1 L −1 m −2 h −1 bar −1 ), high dye rejection (99.4% for 200 ppm of Congo Red), and low salt rejection (4.2% for NaCl and 9.8% for Na 2 SO 4 ). The chemical compositions, surface properties, and morphologies of the LNF membranes were characterized to elucidate their structure–property-performance relationships. The homogeneous diffusion of resveratrol to the water/hexane interface and the appropriate stoichiometry of the two monomers contribute to the formation of an ultra-thin (35–45 nm) and hydrophilic composite membrane, which allows the membrane to attain a high water permeance. The LNF membrane additionally maintains excellent anti-fouling properties during filtration of organic dye solutions, primarily as a result of the high hydrophilicity, negative charge density, and smoothness of the membrane surface. Importantly, the novel LNF membrane also retains stability after washing with ethanol and acetone, owing to the cross-linked polyphenol ester and the rigid phenyl backbone of the membrane surface. These results reveal the huge potential of applying resveratrol-based LNF membranes for the treatment of saline textile wastewater.
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Materials Today Advances
·
2022
·
IF 9.918
Zhiru Zou,Shan Wen,Yingqiao Li,Jinyu An,Qian Wu,Le Tong,Xifan Mei,He Tian,Chao Wu
Stroke is a central nervous system disease with a high incidence at present. It can cause pathological changes such as oxidative stress, inflammation, neuronal apoptosis, and lead to disturbance of consciousness, disability, and even death. Due to the existence of the blood-brain barrier (BBB), most of the drugs cannot reach the lesions and exert their efficacy fully. Therefore, the purpose of this research was to design a lactoferrin functionalized hollow mesoporous manganese doped silica nanoparticles (LHMMSN) for delivery of insoluble drug resveratrol (RES). The system could help insoluble drugs pass through the BBB and target inflammatory sites to exert the antioxidant stress, anti-inflammatory and neuroprotective effects. The RES was adsorbed into LHMMSN with a particle size of about 140 nm by adsorption method, and the drug loading reached 39.32 ± 2.46%. In vitro and in vivo experiments showed that LHMMSN-RES could increase the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), reduce brain reactive oxygen species (ROS) and malondialdehyde (MDA) content, increase the expression of anti-inflammatory factors (IL-10) and anti-apoptotic factors (bcl-2) in brain tissue, and reduce brain tissue pro-inflammatory factors (TNF-α, IL-1β, IL-6) and pro-apoptotic factors (BAX, Cleaved caspase-3) expression. In summary, LHMMSN-RES can reduce inflammation and protect nerve cells by inhibiting oxidative stress, thereby promoting the recovery of motor function in middle cerebral artery occlusion (MCAO) model rats.
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Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 25
·4840-4855
·
IF 7.571
Yujie Tan,Jing Feng,Yu Xiao,Chongyun Bao
Host-modulation therapy is generally accepted as a novel promising method for diabetic periodontitis (DP) treatment and screening an appropriate drug model is the key to success. Resveratrol (RSV), because of its viable antioxidative and anti-inflammatory properties and its ability to control glucose metabolism, is considered a potential candidate. However, poor water solubility, rapid decomposition and short serum half-life period significantly limit its application. Therefore, in this study, we designed a RSV-grafted mesoporous silica nanoparticle (MSN-RSV) drug carrier system to enhance RSV's stability effectively and prolong its duration. Further analyses have verified the indispensable role of MSNs in improving the bioavailability of RSV, which could result in a more favorable therapeutic efficacy in DP related to regulating the polarization of the macrophage. The reason for this could be explained by activating the SIRT1/AMPK signaling pathway and inhibiting the NF-κB signaling pathway. This study also focused on the auxiliary effect of MSN-RSV on alleviating insulin resistance (IR) and controlling glucose metabolism. In brief, the study has provided a potential alternative strategy for DP therapy. It is also helpful for future intensive research topics like the immunoregulatory mechanisms in the bidirectional relationship between diabetes and periodontitis.
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