MOLECULAR BIOTECHNOLOGY
·
2023
·
IF 2.6
Lv Chen-Guang,Cheng Yao,Zhang Lei,Wu Guo-Gang,Liang Chun-Yan,Tao Zuo,Chen Bo
BC (breast cancer) is the leading cause of cancer death in women. Exosome component 2 (EXOSC2), an RNA exosome component, is elevated in BC tissues and may relate to BC carcinogenesis. In this work, the high EXOSC2 expression was correlated with TNM (Tumor Node Metastasis) stage. Moreover, overexpression of EXOSC2 enhanced tumorigenic capacity of BC cells via facilitating cell proliferation and cell cycle progression, increasing migration and angiogenesis, as well as exacerbating xenograft formation in vivo. Whereas, EXOSC2 knockdown showed anti-cancer effects, including inhibition of cell proliferation and angiogenesis. Mechanistically, EXOSC2 activated the wnt/β-catenin pathway, which was also abolished by EXOSC2 knockdown. In addition, there were m6A methylation modification sites in the mRNA of EXOSC2. WTAP (Wilms tumor 1-associated protein) bound to EXOSC2 mRNA and increased its m6A methylation, resulting in extending the half-life of EXOSC2 mRNA. Luciferase data also confirmed that WTAP enhanced EXOSC2 mRNA stability through binding with the 3′-UTR containing m6A sites. Furthermore, WTAP silencing exhibited cancer-inhibiting effects on cell viability, cell cycle progression and tube formation, which was effectively reversed by EXOSC2 overexpression. In conclusion, our results demonstrate that EXOSC2 promotes the malignant behaviors of BC cells via activating the wnt/β-catenin pathway. In addition, EXOSC2 mediates the function of WTAP which contributes to the m6A modification of EXOSC2. Totally, this study suggested that EXOSC2 mediated the pro-tumor role of WTAP via activating the wnt/β-catenin signal.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 28
·1-11
·
IF 2.8
Xia Junjia,Ma Xing-Hua,Zhang Zhenlu,Nahm Sahn,Zhang Shuling,Ma Xingyi,Guo Feng
In this work, ZnGa 2– x O 4–1.5 x (0.0 ≤ x ≤ 0.15) ceramics were synthesized by a solid-state reaction method. ZnGa 2 O 4 ceramic that is sintered at 1300 °C/3 h exhibits good microwave dielectric properties with ε r of 11.06, Q × f of 74,730 GHz, and τ f of − 69.7 ppm/°C. However, it cannot be fully densified at temperatures lower than 960 °C, even when a large amount of B 2 O 3 is added, which seriously inhibits its application in the LTCC (low temperature cofired ceramic) field. Therefore, adding B 2 O 3 to Ga 2 O 3 -deficient ZnGa 2 O 4 ceramics is proposed to decrease the sintering temperature to below 960 °C. Owing to the dual effect of the B 2 O 3 -rich and ZnO-B 2 O 3 related liquid phases, ceramics with added B 2 O 3 (≥ 20 mol%) were found to sinter well at 950 °C, and good microwave dielectric properties were also achieved. In particular, 25 mol% B 2 O 3 added to ZnGa 1.95 O 3.925 ceramic sintered at 950 °C showed promising microwave dielectric properties for 5 G/6 G technologies without reaction with a silver electrode: ε r = 9.19, Q × f = 37,337 GHz, and τ f = − 58.3 ppm/°C.
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Nano Research
·
2023
·
IF 9.9
Hu Dejia,Jiang ShangZuo,Xia Tianhao,Xiao Danyang,Li Yan,Hou Yanbing,Zhang Jin Zhong,Pu Ying-Chih
Ti 2 C quantum dots (QDs) with rich surface functional groups have been synthesized using a hydrothermal method, and used to detect tetracycline (Tc) based on enhanced fluorescence. The interaction between the surface functional groups of Ti 2 C QDs and Tc enhanced the fluorescence of Tc at 514 nm, which is used to detect Tc quickly and accurately. Under optimal conditions, the fluorescence intensity was linear to the concentration of Tc in the range of 50.0–30.0 μM, with a detection limit of 21.6 nM. Furthermore, the Tc-Ti 2 C QDs detection system was evaluated for detection of Tc in milk and artificial urine. This study demonstrates a new and simple strategy for Tc detection, which is important for food safety and human health.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 11
·1-9
·
IF 5.7
Yao Yuying,Wang Yeru,Zhu Jingru,Guo Zhiqiang,Li Zongqiang,Li Qianru,Liu Su,Wang Yu,Yu Jinghua,Huang Jiadong
A DNA triangular prism nanomachine (TPN)–based logic device for intracellular AND-gated imaging of adenosine triphosphate (ATP) has been constructed. By using i-motif sequences and ATP-binding aptamers as logic control units, the TPN logic device is qualified to respond to the acidic environment and ATP in cancer cell lysosomes. Once internalized into the lysosome, the specific acidic microenvironment in lysosome causes the i-motif sequence to fold into a tetramer, resulting in compression of DNA tri-prism. Subsequently, the split ATP aptamer located at the tip of the collapsed triangular prism binds stably to ATP, which results in the fluorescent dyes (Cy3 and Cy5) modified at the ends of the split aptamer being in close proximity to each other, allowing Förster Resonance Energy Transfer (FRET) to occur. The FRET signals are excited at a wavelength of 543 nm and can be collected within the emission range of 646–730 nm. This enables the precise imaging of ATP within a cell. We also dynamically operate AND logic gates in living cells by modulating intracellular pH and ATP levels with the help of external drugs. Owing to the AND logic unit on TPN it can simultaneously recognize two targets and give corresponding intelligent logic judgment via imaging signal output. The accuracy of molecular diagnosis of cancer can be improved thus eliminating the false positive signal of single target-based detection. Hence, this space-controlled TPN-based logical sensing platform greatly avoids sensitivity to extracellular targets during the cell entry process, providing a useful tool for high-precision imaging of the cancer cell’s endogenous target ATP.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2023
·Vol. 46
·期 12
·1801-1815
·
IF 3.8
Zhou Zhaoxin,Liu Xinmin,Chen Ranran,Hu Xiude,Guo Qingjie
Anaerobic fluidized bed microbial fuel cell (AFB-MFC) is a technology that combines fluidized bed reactor and microbial fuel cell to treat organic wastewater and generate electricity. The performance and the mechanism of treating m- cresol wastewater in AFB-MFC using carbon brush as biofilm anode were studied. After 48 h of operation, the m- cresol removal efficiency of AFB-MFC, MAR-AFB (fluidized bed bioreactor with acclimated anaerobic sludge), MAR-FB (ordinary fluidized bed reactor with only macroporous adsorptive resin) and AST (traditional anaerobic sludge treatment) were 95.29 ± 0.67%, 85.78 ± 1.81%, 71.24 ± 1.86% and 70.41 ± 0.32% respectively. The maximum output voltage and the maximum power density of AFB-MFC using carbon brush as biofilm anode were 679.7 mV and 166.6 mW/m 2 respectively. The results of high-throughput sequencing analysis indicated the relative abundance of dominant electroactive bacteria, such as Trichococcus , Geobacter , and Pseudomonas , on the anode carbon brushes was higher than that of AST, and also identified such superior m- cresol-degrading bacteria as Bdellovibrio , Thermomonas , Hydrogenophaga , etc. Based on the determination of m- cresol metabolites detected by Gas Chromatography–Mass Spectrometry (GC–MS), the possible biodegradation pathway of m- cresol under anaerobic and aerobic conditions in AFB-MFC was speculated. The results showed that m- cresol was decomposed into formic acid–acetic anhydride and 3-methylpropionic acid under the action of electrochemistry, which is a simple degradation pathway without peripheral metabolism in AFB-MFC.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-19
·
IF 16.6
Song Jianwen,Kang Xiaoying,Wang Lu,Ding Dan,Kong Deling,Li Wen,Qi Ji
The formation of an occlusive thrombus in the blood vessel is the main culprit for numerous life-threatening cardiovascular diseases that represent the leading cause of morbidity and mortality worldwide. Herein, we develop a polymer nanoplatform that integrates long-wavelength second near-infrared (NIR-II) photoacoustic imaging-based thrombosis detection and antithrombotic activity. We design and synthesize a semiconducting homopolymer with strong absorption in the NIR-II region and molecular motion that boosts photothermal conversion and photoacoustic signal. We dope the homopolymer with a thermosensitive nitric oxide donor to formulate a nanoplatform, on which a fibrin-specific ligand is functionalized to ensure selective thrombus targeting. We show that with strong NIR-II light harvesting capability, bright photoacoustic signal and active thrombus accumulation ability, the NIR-II photoacoustic nanoprobes are able to sensitively and selectively delineate thrombi. We find that the nanoplatform also displays rapid and efficient blood clot removal activity with nearly complete blood flow restoration in both carotid thrombosis models and low extremity arterial thrombosis models under NIR-II light trigger by integrating a thrombus-localized photothermal effect and on-demand nitric oxide release. This nanoplatform offers a versatile approach for the diagnosis and treatment of life-threatening diseases caused by various thrombotic disorders.
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Nano-Micro Letters
·
2023
·Vol. 15
·期 1
·1-20
·
IF 26.6
He Hualing,Qin Yi,Zhu Zhenyu,Jiang Qing,Ouyang Shengnan,Wan Yuhang,Qu Xueru,Xu Jie,Yu Zhicai
The p – n segment thermoelectric aerogel fiber was fabricated through an alternating coaxial wet-spinning strategy.
Resultant alternating p – n segment thermoelectric fiber was electrically connected in series (two p – n pairs with a length of 3 cm) with an outstanding electrical conductivity of 23.76 S m −1 .
Thermoelectric textile-based self-powered fire warning electronics exhibited sensitivity (trigger time within 1.43 s) and repeatable temperature sensing performance for firefighting clothing.
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Food and Bioprocess Technology
·
2023
·
IF 5.6
Liu Xinru,Ma Qianwei,Sun Yongjun,Ju Wenming,Kumrungsee Thanutchaporn,Zhou Zhongkai,Wang Lijuan,Cao Ruge
To take better advantage of buckwheat polyphenols, processings including extrusion, steam explosion, microwave, roasting, superheated steam, ultrasound and enzymolysis treatment were applied to buckwheat bran to release more bioactive polyphenols. Polyphenols were extracted and characterized, and rutin was identified as the predominant compound. To further improve the properties of buckwheat polyphenols, rutin was selected as the target polyphenol and a formation of its ionic cross-linking complex was proposed. Rutin was loaded on chitooligosaccharide as Rutin-COS with tripolyphosphate (TPP) as crosslinking agent, whose molecular structure and properties were investigated. Results showed that all processing significantly increased ( p < 0.05) the content of buckwheat polyphenols, up to 1.83-fold increase. The composition of polyphenols was altered, and their antioxidant (up to 15% increase) and antibacterial activities were significantly enhanced ( p < 0.05). Among all the processings, extrusion and superheated steam showed the best effect. For Rutin-COS complex, the encapsulation efficiency was as high as 74.77% with the mass ratio of rutin and COS at 1:8. The structure of Rutin-COS was stabilized by hydrogen bonding between aromatic rings of rutin and amino and hydroxy groups of COS in the network formed by COS and TPP. Compared to free rutin, Rutin-COS presented better thermal stability and water solubility. The antioxidant and antibacterial activities of Rutin-COS were also significantly improved ( p < 0.05) due to the molecular interaction between rutin and COS. The combination of processing and complexation strategy for buckwheat polyphenols in this work provided new approaches to take better advantages of polyphenols of cereal foods. Graphical Abstract
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FIBERS AND POLYMERS
·
2023
·Vol. 24
·期 12
·4269-4277
·
IF 2.5
Zhao Qiang,Zhang Yunting,Liu Zhenjiang,Ma Huiyan,Li Yan,Gao Xiaoping
TiO 2 /kaolin was first synthesized by using ultrasound-sol–gel method and then deposited onto the surface of cotton fabrics through two dipping and two rolling process. To investigate the properties of this TiO 2 /kaolin-finished cotton fabric, TiO 2 /kaolin/cotton composites were characterized by Fourier transform infrared spectrum and energy dispersive spectrometer, while the surface morphologies of the cotton fabrics were analyzed by field emission scanning electron microscopy. The whiteness, air permeability, breaking strength and elongation of TiO 2 /kaolin/cotton composites were, respectively, measured and the results showed that all these parameters decreased slightly with the appearance of TiO 2 /kaolin. The UPF value of TiO 2 /kaolin/cotton composites was significantly enhanced to 100+ , even if after soaping for 30 times, which might be due to the synergistic effect of kaolin and TiO 2 . In addition, the performance such as hand feel attributes, softness, smoothness and stiffness were measured by PhabrOmeter, respectively. The stiffness of all finished fabrics was obviously better than that of the unfinished cotton. However, the softness and smoothness of finished fabrics were slightly decreased compared to unfinished cotton. Rhodamine B was used as a test contaminant to qualitatively assess the self-cleaning properties of the TiO 2 /kaolin-finished cotton fabric. The removal of Rhodamine B indicated that the TiO 2 /kaolin-finished fabric exhibited excellent photocatalytic properties. This study illustrates the great potential of the low-cost kaolin powder as an efficient finishing agent for the fabric finishing process, and meanwhile provides a novel suggestion for fabric modification.
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Nano Research
·
2023
·Vol. 16
·期 11
·12727-12737
·
IF 9.9
Liu Hongbin,Sun Qing,Cheng Jun,Zhang Hongqiang,Xu Xiao,Li Yuanyuan,Zeng Zhen,Zhao Yue,Li Deping,Lu Jingyu,Ci Lijie
Due to the increasing demand and wide applications of lithium-ion batteries, higher requirements have been placed on the energy density and safety. Polymer solid-state electrolytes have gained significant popularity due to their excellent interface compatibility and safety. However, their applications have been greatly restricted by the high crystallinity at room temperature, which hinders the transport of lithium ions. Herein, we utilize inorganic tubular fillers with abundant lone-pair atoms to reduce the crystallinity of the polyethylene oxide (PEO) solid-state electrolyte membrane and improve its ionic conductivity at room temperature, enabling stable operation of the battery. The tubular lone-pair-rich inorganic fillers play a key role in providing avenues for both internal and external charge transportation. The surface lone-pair electrons facilitate the dissociation and transport of lithium ions, while the internally tubular electron-rich layer attracts ions into the cavities, further enhancing the ion transport. After 100 cycles at room temperature, the lithium battery loaded with this solid-state electrolyte membrane delivers a specific capacity of 141.6 mAh·g −1 , which is 51.3% higher compared to the membrane without the fillers.
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Chinese Medicine
·
2023
·Vol. 18
·期 1
·1-16
·
IF 4.9
Wang Xiaohu,Shen Chaozhuang,Wang Xingwen,Tang Jin,Wu Zijing,Huang Yunzhe,Shao Wenxin,Geng Kuo,Xie Haitang,Pu Zhichen
Background According to the Chinese Pharmacopoeia, the fruit of Schisandra chinensis (Turcz.) Baill. (SC) is an important traditional Chinese medicine that can be used to treat diarrhea. Despite the increasing research on the anti-inflammatory and anti-oxidant aspects of SC, the studies on the anti-ulcerative colitis of Schisandrin (SCH), the main constituent of SC, are relatively few.Methods The mice used in the study were randomly distributed into 6 groups: control, model, 5-ASA, and SCH (20, 40, 80 mg/kg/d). The mice in the model group were administered 3% (w/v) dextran sulfate sodium (DSS) through drinking water for 7 days, and the various parameters of disease activity index (DAI) such as body weight loss, stool consistency, and gross blood were measured. ELISA was used to detect inflammatory factors, and bioinformatics combined with transcriptome analysis was done to screen and verify relevant targets. 16S rDNA high-throughput sequencing was used to analyze the composition of the gut microbiota(GM), while mass spectrometry was done to analyze the changes in the content of bile acids (BAs) in the intestine.Results Mice treated with SCH experienced significant weight gain, effectively alleviating the severity of colitis, and decreasing the levels of inflammatory factors such as TNF-α, IL-1β, IL-18, IL-6, and other related proteins (NLRP3, Caspase-1, SGK1) in UC mice. Furthermore, the analysis of GM and BAs in mice revealed that SCH increased the relative abundance of Lactobacilli spp, reduced the relative abundance of Bacteroides, and promoted the conversion of primary BAs to secondary BAs. These effects contributed to a significant improvement in the DSS-induced GM imbalance and the maintenance of intestinal homeostasis.Conclusion It seems that there is a close relationship between the SCH mechanism and the regulation of SGK1/NLRP3 pathway and the restoration of GM balance. Therefore, it can be concluded that SCH could be a potential drug for the treatment of UC.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-18
·
IF 16.6
Zhong Jing,He Xiaofang,Gao Xinxin,Liu Qiaohong,Zhao Yu,Hong Ying,Zhu Weize,Yan Juan,Li Yifan,Li Yan,Zheng Ningning,Bao Yiyang,Wang Hao,Ma Junli,Huang Wenjin,Liu Zekun,Lyu Yuanzhi,Ke Xisong,Jia Wei,Xie Cen,Hu Yiyang,Sheng Lili,Li Houkai
Nonalcoholic fatty liver disease (NAFLD) is usually characterized with disrupted bile acid (BA) homeostasis. However, the exact role of certain BA in NAFLD is poorly understood. Here we show levels of serum hyodeoxycholic acid (HDCA) decrease in both NAFLD patients and mice, as well as in liver and intestinal contents of NAFLD mice compared to their healthy counterparts. Serum HDCA is also inversely correlated with NAFLD severity. Dietary HDCA supplementation ameliorates diet-induced NAFLD in male wild type mice by activating fatty acid oxidation in hepatic peroxisome proliferator-activated receptor α (PPARα)-dependent way because the anti-NAFLD effect of HDCA is abolished in hepatocyte-specific Pparα knockout mice. Mechanistically, HDCA facilitates nuclear localization of PPARα by directly interacting with RAN protein. This interaction disrupts the formation of RAN/CRM1/PPARα nucleus-cytoplasm shuttling heterotrimer. Our results demonstrate the therapeutic potential of HDCA for NAFLD and provide new insights of BAs on regulating fatty acid metabolism.
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