JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Mengfei Li,Hongyu Chen,Dingchuan Peng,Xuehan Lu,Jianping Kong,Shujun Luo,Shiqi Li,Chunlu Tan,Ying Wang
A biodegradable drug delivery system with pH-sensitive property holds vast promise for the improvement of gemcitabine (GEM) in pancreatic cancer cells. Here, a cationic pH-sensitive liposome containing GEM with FU coating (pPSL-FU-GEM, DOTAP: CHEMS : Chol: DSPE-mPEG2000) was designed to improve the bioavailability and efficacy of GEM. After entering the acidic environment of cells, pPSL-FU-GEM could release gemcitabine through an endocytic escape mechanism, preventing it from being degraded by enzymes. The negatively charged fucoidan (FU), which was separated and purified by anion exchange column purification, could target to pancreatic cancer cells and shed, resulting in liposomes uptake by cells. Release studies and intracellular trafficking studies showed that pPSL-FU-GEM released more drug than non-pH-sensitive liposomes in vitro . Meanwhile, pPSL-FU-GEM also showed better anti-proliferative activity than free gemcitabine in pancreatic cancer cells. Overall, we demonstrated the potential of FU-coating pH-sensitive liposomes containing gemcitabine and the drug delivery function in pancreatic cancer cells.
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高影响因子
ACS Nano
·
2023
·
IF 17.1
Xuchun Chen,Ying Cheng,Qi Pan,Lan Wu,Xinyao Hao,Zhiye Bao,Xitan Li,Mingshi Yang,Qiuhua Luo,Heran Li
Chiral nanoparticles (NPs) with nanoscale rough surfaces have enormous application prospects in drug delivery. However, the stereoselective interactions between the chiral NPs and biosurfaces remain challenging and mysterious. Herein, we designed mesoporous silica nanocarriers (l/d/dl-TA-PEI@CMSN) exhibiting the same structural parameters (hydrophilic, electroneutral, spherical NPs, ∼120 nm) but different geometrical chirality as oral nanodrug delivery systems (Nano-DDS) for insoluble drugs nimesulide (NMS) and ibuprofen (IBU) and demonstrated their stereoselective interactions with the intestinal mucosa, that is, l-TA-PEI@CMSN as well as Nano-DDS in the l-configuration displayed apparent superior behaviors in multiple microprocesses associated with oral adsorption, including adhesion, penetration, adsorption, retention and uptake, causing by the stereomatching between the chiral mesostructures of NPs and the inherent chiral topologies of the biosurfaces. As hosting systems, l/d/dl-TA-PEI@CMSN effectively incorporated drugs in amorphous states and helped to overcome the stability, solubility and permeability bottlenecks of drugs. Subsequently, Nano-DDS in the l-configuration (including IBU/l-TA-PEI@CMSN and NMS/d-TA-PEI@CMSN owing to a chiral inversion) showed higher oral delivery efficiency of NMS and IBU evidenced by the larger relative bioavailability (1055.06% and 583.17%, respectively) and stronger anti-inflammatory and analgesic effects. In addition, l/d/dl-TA-PEI@CMSN were stable, nonirritative, biocompatible and biodegradable, benefiting for their clinical applications. These findings provided insights into the rational design of functionalized Nano-DDS and contributed to the further knowledge in the field of chiral pharmaceutical science.
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高影响因子
Biofabrication
·
2023
·Vol. 15
·期 2
·025010
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IF 9
Xin Sun,Jin Yang,Jie Ma,Tianchang Wang,Xue Zhao,Dan Zhu,Wenjie Jin,Kai Zhang,Xuzhou Sun,Yuling Shen,Neng Xie,Fei Yang,Xiushuai Shang,Shuai Li,Xiaojun Zhou,Chuanglong He,Deteng Zhang,Jinwu Wang
The periosteum is a connective tissue membrane adhering to the surface of bone tissue that primarily provides nutrients and regulates osteogenesis during bone development and injury healing. However, building an artificial periosteum with good adhesion properties and satisfactory osteogenesis for bone defect repair remains a challenge, especially using three-dimensional (3D) bioprinting. In this study, dopamine was first grafted onto the molecular chain of gelatin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) to activate the carboxyl group and produce modified gelatin-dopamine (GelDA). Next, a methacrylated gelatin, methacrylated silk fibroin, GelDA, and graphene oxide nanosheet composite bioink loaded with bone marrow mesenchymal stem cells was prepared and used for bioprinting. The physicochemical properties, biocompatibility, and osteogenic roles of the bioink and 3D bioprinted artificial periosteum were then systematically evaluated. The results showed that the developed bioink showed good thermosensitivity and printability and could be used to build 3D bioprinted artificial periosteum with satisfactory cell viability and high adhesion. Finally, the 3D bioprinted artificial periosteum could effectively enhance osteogenesis both in vitro and in vivo. Thus, the developed 3D bioprinted artificial periosteum can prompt new bone formation and provides a promising strategy for bone defect repair.
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高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Rui Jiao,Yanming Wang,Yuxia Pang,Dongjie Yang,Zhixian Li,Hongming Lou,Xueqing Qiu
Metal–organic frameworks (MOFs) have become promising accommodation for enzyme immobilization in recent years. However, the microporous nature of MOFs affects the accessibility of large molecules, resulting in a significant decline in biocatalysis efficiency. Herein, a novel strategy is reported to construct macroporous MOFs by metal competitive coordination and oxidation with induced defect structure using a transition metal (Fe2+) as a functional site. The feasibility of in situ encapsulating β-glucosidase (β-G) within the developed macroporous MOFs endows an enzyme complex (β-G@MOF-Fe) with remarkably enhanced synergistic catalysis ability. The 24 h hydrolysis rate of β-G@MOF-Fe (with respect to cellobiose) is as high as approximately 99.8%, almost 32.2 times that of free β-G (3.1%). Especially, the macromolecular cellulose conversion rate of β-G@MOF-Fe reached 90% at 64 h, while that of β-G@MOFs (most micropores) was only 50%. This improvement resulting from the expansion of pores (significantly increased at 50–100 nm) can provide enough space for the hosted biomacromolecules and accelerate the diffusion rate of reactants. Furthermore, unexpectedly, the constructed β-G@MOF-Fe showed a superior heat resistance of up to 120 °C, attributing to the new strong coordination bond (Fe2+–N) formation through the metal competitive coordination. Therefore, this study offers new insights to solve the problem of the high-temperature macromolecular substrate encountered in the actual reaction.
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高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Tian Zhang,Qiang Zheng,Congkun Xie,Gonglin Fan,Yifan Wang,Yongjun Wu,Yike Fu,Jie Huang,Duncan Q. M. Craig,Xiujun Cai,Xiang Li
As a result of their radiation-free nature and deep-penetration ability, tumor theranostics mediated by ultrasound have become increasingly recognized as a modality with high potential for translation into clinical cancer treatment. The effective integration of ultrasound imaging and sonodynamic therapy (SDT) into one nanoplatform remains an enormous challenge yet to be fully resolved. Here, a novel theranostic system, consisting of rattle-type SiO2 (r-SiO2) loaded with Mn-doped In2S3/InOOH (SMISO), was designed and synthesized to enable an improved ultrasound imaging-guided therapy. With Mn-doped In2S3/InOOH (MISO) and a heterojunction structure, this novel sonosensitizer facilitates the generation of reactive oxygen species (ROS) for SDT. By coupling interfaces between the shell and core in rattle-type SiO2, multiple reflections/scattering are generated, while MISO has high acoustic impedance. By integrating r-SiO2 and MISO, the SMISO composite nanoparticles (NPs) increase the acoustic reflection and provide enhanced contrast for ultrasound imaging. Through the effective accumulation in tumors, which was monitored by B-mode ultrasound imaging in vivo, SMISO composite NPs effectively inhibited tumor growth without adverse side effects under ultrasound irradiation treatment. This work therefore provides a new approach to integrate a novel gas-free ultrasound contrast agent and a semiconductor sonosensitizer for cancer theranostics.
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高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 8
·2392-2403
·
IF 4
Amir Said,Guanyun Zhang,Caiyun Liu,Dexin Wang,Huihui Niu,Yanshu Liu,Guanjie Chen,Chen-Ho Tung,Yifeng Wang
The cycloaddition reaction of CO2 to epoxides is quite promising for CO2 capture and storage as well as the production of value-added fine chemicals. Herein, a novel atomically precise lead-doped titanium-oxide cluster with the formula Ti10Pb2O16(phen)4(Ac)12(DMF)2 (denoted as Ti10Pb2; phen = 1,10-phenanthroline; Ac = acetate; DMF = dimethylformamide) was synthesized through a facile solvothermal process, and is a molecular photocatalyst with surface-anchored main-group metal active sites. Its structure was characterized by single-crystal X-ray diffraction and other complementary techniques. Ti10Pb2 showed high photo-response and charge-separation efficiency under simulated sunlight irradiation. Ti10Pb2 was successfully used in the cycloaddition reaction of CO2 with epoxides under solvent-free conditions. While its catalytic activity due to the Lewis acidity was moderate, simulated solar light irradiation further enhanced the reaction rate, demonstrating the synergistic effect of photocatalysis and Lewis-acid thermocatalysis.
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高影响因子
Acta Biomaterialia
·
2023
·
IF 9.7
Dawei Jiang,Luqi Pan,Xiao Yang,Zhixiao Ji,Cheng Zheng,Zhizhen Meng,Bin Liang,Weian Zhang,Jinfei Chen,Changcan Shi
The newly attractive peroxynitrite (ONOO − ) therapy can prominently enhance antibacterial therapeutic efficacy. However, it is a great challenge but urgently needed to generate ONOO − with adjustable release rate and dosage in order to satisfy personalized treatments for different disease types and severities. Herein, PSNO@IR780 nanoparticles are fabricated via co-assembly of an amphiphilic PEG- b -PAASNO block copolymer grafted with abundant nitric oxide (NO) donor units and IR780 as a photothermal and photodynamic agent. Photo-controllable burst generation of ONOO − from PSNO@IR780 nanoparticles could be realized based on synergistic reactions of rapid NO release induced by increased local temperature and efficiently produced superoxide anion radical (O 2 •− ) from IR780. The maximum ONOO − release dosage is up to 6.73 ± 0.07 µM and release rate is up to 98.1 ± 1.38 nM/s. Furthermore, the ONOO − release behavior can be precisely manipulated by varying sample concentrations, irradiated durations, output power densities, and laser switches, respectively. Ultra-efficiently generated ONOO − from biocompatible PSNO@IR780 nanoparticles significantly elevated broad spectrum antibacterial efficiency through damaging bacterial membranes . Thus, PSNO@IR780 nanoparticles may present a new insight into preparation of burst and controllable generating ONOO − materials, and provide new opportunities for antibacterial therapy. Statement of significance 1. Polymeric NO donor (PEG- b -PAASNO) grafted with abundant NO donor units was synthesized. 2. PSNO@IR780 nanoparticles were prepared by co-assembly of IR780 and amphiphilic PEG- b -PAASNOpolymer. 3. The maximum ONOO − release dosage from PSNO@IR780 nanoparticles was 6.73 ± 0.08 µM. 4. The fastest ONOO − release rate from PSNO@IR780 nanoparticles was 98.1 ± 1.4 nM/s. 5. Ultra-efficiently generated ONOO − significantly elevated antibacterial efficiency via damaging bac-terial membranes.
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高影响因子
Food Analytical Methods
·
2023
·Vol. 16
·期 4
·689-698
·
IF 2.9
Chu Qiqi,Wu Xinze,Chen Huaixia,Dang Xueping,Liu Xiaolan
A Cu 7 Zn 2 cluster functionalized copolymer monolithic column was prepared and applied as the adsorbent of pipette tip micro-solid phase microextraction of eight phthalate esters (PAEs). The physical morphology, chemical composition, thermal stability, and specific surface area of the monolithic column were characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, thermogravimetric analysis, and N 2 adsorption and desorption experiment. Cu 7 Zn 2 cluster acted as the adsorption site effectively improved the adsorption selectivity and capacity of the copolymer monolith for PAEs. The adsorption mechanism of Cu 7 Zn 2 copolymer monolith for 8 PAEs was deduced to be mainly the H-bond interaction. And, the chemical doping and copolymerization of Cu 7 Zn 2 cluster made the composite monolithic column more stable with a long service life. Under the optimized extraction conditions, a micro-solid phase extraction-high performance liquid chromatography method with ultraviolet detector (μ-SPE-HPLC-UV) was developed for the determination of 8 PAEs in edible oil. Wide linear ranges of 0.5–1000 μg/kg with correlation coefficients ( R 2 ) above 0.996 were obtained. The limits of detection (LODs) were in the range of 0.1–0.9 μg/kg. The proposed method was applied for the detection of 8 PAEs in edible oil with satisfactory recoveries between 83.5~100.2%, and the relative standard deviations were less than 8.1%. The results indicated that the Cu 7 Zn 2 functionalized copolymerized monolithic column was an ideal enrichment medium for the detection of PAEs in complex samples.
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高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 8
·2335-2344
·
IF 4
Jie Jin,Shanshan Wu,Jing Wang,Yunqi Xu,Shouhu Xuan,Qunling Fang
In this work, a MXene@AgPd/polydopamine (PDA) nanosheet with excellent photothermal conversion efficiency was successfully synthesized by a simple redox-oxidative polymerization method. Interestingly, AgPd bimetallic nanocrystals sandwiched between a MXene nanosheet and PDA layer have cage-like nanostructure, which is favorable for high catalytic efficiency and antibacterial performance. Importantly, the MXene@AgPd/PDA nanosheet exhibits good catalytic activity for the reduction of 4-nitrophenol (1.2 min−1 mg−1) and the catalytic dynamics can be improved by about 1.2 times under NIR (near-infrared light, 808 nm, and 2.5 W cm−2) irradiation. As the PDA shell is well protected, the MXene@AgPd/PDA nanosheet retained more than 90% catalytic activity after 6 cycles. In addition, due to the presence of the Ag component, the MXene@AgPd/PDA nanosheet exhibited good antibacterial activity against both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Under near-infrared light irradiation, its antibacterial activity was further enhanced due to the NIR photothermal effect.
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高影响因子
Advanced Therapeutics
·
2023
·Vol. 6
·期 4
·2200304
·
IF 4.6
Dan Zhao,Min Wu,Yanan Tian,Lu Gao,Fu Wang
With the advantages of ferroptosis therapy (FT) in solving the apoptotic resistance of tumors, a smart strategy based on the combination of glucose oxidase (GOx) and ferroptosis-inducing nanoagents is proposed to boost the FT. However, most of the nanoagents remain limited in complex synthesis, poor targeting specificity, and unclarified biological mechanisms. Herein, a multifunctional nanoplatform based on fluorescent apoferritin-gold nanoclusters combined with GOx (AFt-AuNCs@GOx) is designed for tumor-targeted imaging and FT. The results show that AFt-AuNCs@GOx catalyze the generation of toxic ·OH from H 2 O 2 supplied by GOx. Consequently, AFt-AuNCs@GOx induce the enhanced ferroptosis effect in 4T1 cells. Additionally, the transcriptome assay of 4T1 cells identifies the differential expression genes related to ferroptosis process after AFt-AuNCs@GOx treatment. Moreover, with the merit of intrinsic fluorescence, the biodistribution both in vitro and in vivo of AFt-AuNCs@GOx can be effectively visualized. In vivo evidences reveal that AFt-AuNCs@GOx have the desired efficacy of FT and excellent biosafety. Overall, the AFt-AuNCs@GOx nanoplatform offer remarkable advantages in simple architecture, fluorescent imaging, precise targeting, and tumor inhibition. The exploration of the ferroptosis effect and biological mechanisms of AFt-AuNCs@GOx, as well as its application in tumor therapy, can introduce a new insight into the field of ferroptotic nanomedicine.
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高影响因子
Colloid and Interface Science Communications
·
2023
·
IF 4.5
Chengyang Ban,Hanzhu Shi,Qiong Ding,Bin Wu,Ru Xia,Jiasheng Qian,Ming Cao
In this study, Fe/ABTS/PVP nanocomplexes (named FAPs) as photothermal-reinforced catalytic antibacterial agents were prepared using the coordination-induced assembly of Fe (III), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and polyvinylpyrrolidone (PVP). The redox pairs of Fe(II)/Fe(III) in FAPs can catalyze the H 2 O 2 to hydroxyl radicals (•OH) through Fenton reactions, thereby killing effect on bacteria. The photothermal effect of ABTS •+ in FAPs stimulated by near infrared (NIR) irradiation can further improve the catalytic effect, thereby generating more •OH and achieving better antibacterial activity. What's more, the reduction reaction between glutathione (GSH) and ABTS •+ results in the depletion of the GSH level, the redox pair of Fe(II)/Fe(III) is also beneficial in the consumption of GSH. This dual depletion of GSH further expands the intracellular oxidative stress. Thus, the integrated function of photothermal effect, catalytic property, and dual depletion of GSH by FAPs provides a new strategy for fighting against bacterial infections.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 3
·1391
·
IF 4.6
Yunyi Liang,Yonghong Luo,Yang Wang,Tianyang Fei,Lili Dai,Daihui Zhang,Hongzhi Ma,Liping Cai,Changlei Xia
Soy protein isolate (SPI) is an attractive natural material for preparing wood adhesives that has found broad application. However, poor mechanical properties and unfavorable water resistance of wood composites with SPI adhesive bonds limit its more extensive utilization. The combination of lysine (Lys) with a small molecular structure as a curing agent for modified soy-based wood adhesive allows Lys to penetrate wood pores easily and can result in better mechanical strength of soy protein-based composites, leading to the formation of strong chemical bonds between the amino acid and wood interface. Scanning electron microscopy (SEM) results showed that the degree of penetration of the S/G/L-9% adhesive into the wood was significantly increased, the voids, such as ducts of wood at the bonding interface, were filled, and the interfacial bonding ability of the plywood was enhanced. Compared with the pure SPI adhesive, the corresponding wood breakage rate was boosted to 84%. The wet shear strength of the modified SPI adhesive was 0.64 MPa. When Lys and glycerol epoxy resin (GER) were added, the wet shear strength of plywood prepared by the S/G/L-9% adhesive reached 1.22 MPa, which increased by 29.8% compared with only GER (0.94 MPa). Furthermore, the resultant SPI adhesive displayed excellent thermostability. Water resistance of S/G/L-9% adhesive was further enhanced with respect to pure SPI and S/GER adhesives through curing with 9% Lys. In addition, this work provides a new and feasible strategy for the development and application of manufacturing low-cost, and renewable biobased adhesives with excellent mechanical properties, a promising alternative to traditional formaldehyde-free adhesives in the wood industry.
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高影响因子
危险品化学品经营许可证（带存储）